1709 results about "Albumin" patented technology

The invention provides an anti-human serum albumin nano antibody and application thereof, and belongs to the technical field of antibodies. The invention provides an anti-human serum albumin nano antibody HSA-32 with relatively high affinity, and the anti-human serum albumin nano antibody HSA-32 is subjected to humanized transformation, so that three obtained humanized anti-human serum albumin nano antibodies can be used for reducing the immunogenicity of the HSA-32 and improving the immunogenicity of the HSA-32; and high specificity and high affinity of combination with the human serum albumin and stability and solubility of the antibody are maintained. The anti-human serum albumin nano antibody can be used for separation, purification and detection of human serum albumin and separation and purification of polypeptide, protein or antibody drugs fused with the anti-human serum albumin nano antibody. Meanwhile, the anti-human serum albumin nano-antibody can effectively prolong the half-life period of polypeptide, protein and antibody drugs fused with the anti-human serum albumin nano-antibody, and can be used for developing long-acting polypeptide, protein or antibody drugs.

The present invention discloses a human serum albumin-targeting nanobody and its application. A variety of human serum albumin-targeting nanobodies that can be used to construct long-acting fusion protein drugs and their immune screening methods are disclosed. The antibody is composed only of the heavy chain variable region and can be fused and expressed with therapeutic proteins or polypeptide drugs such as interleukin, interferon, and tumor necrosis factor in an Escherichia coli expression system. The fusion protein has the activity of specifically binding to human serum albumin, has a relatively high affinity, can effectively extend the drug half-life, and does not affect the biological activity of the recombinant protein drug at the same time.

The invention discloses a bovine serum albumin combined nano antibody and a preparation method and application thereof, the amino acid sequence of the bovine serum albumin combined nano antibody Nb1 is shown as SEQ.ID No.1, the nano antibody Nb not only has high affinity to bovine serum albumin, but also has good binding activity to both human serum albumin and mouse serum albumin, and the nano antibody Nb has high affinity to human serum albumin and mouse serum albumin. And the antibody has a relatively good broad-spectrum application range, and can be widely applied to the aspects of drug half-life period enhancement, tumor targeted therapy and imaging, immunodetection or diagnosis, recombinant albumin purification or enrichment and the like.

Fusion proteins are provided that consist of essential elements such as one or more proteins / polypeptides capable of transporting into the endoplasmic reticulum (ER) and fragments or variants of interleukin 18 (IL-18) or IL-18. The proteins / polypeptides capable of transporting into the ER include, but are not limited to, immune globular proteins (e.g., Fc regions, VHH antibodies, and scFv), and globular proteins (e.g., serum albumin). In some embodiments, the IL-18 may be a pro-IL-18 similar to a natural precursor cytokine, wherein IL-18 is fused to the C-terminus of a propeptide or a propeptide variant thereof. In some embodiments, the fusion protein may further comprise a cleavable peptide linker.

Provided herein are albumin binding proteins and methods of making and using thereof.

The invention discloses a preparation method and application of a cell culture liver based on a microcarrier. The method comprises the steps of obtaining liver seed cells, preparing the microcarrier, culturing cells in vitro based on the microcarrier, preparing the cell culture liver and the like. The preparation method of the cell culture liver based on the microcarrier is provided for the first time, and the preparation scheme that a cell-microcarrier compound serves as a structural unit and is assembled into the cell culture liver after being mixed with an edible material is adopted, so that on one hand, simulation of a real liver lobule structure can be achieved, and on the other hand, simulation of the liver lobule structure can be achieved; the whole structure relation of liver lobules and connective tissues in liver tissues can be simulated, full reduction of animal liver appearance and structural characteristics is achieved, edible materials used in the preparation process are low in price and good in biocompatibility, and the preparation method is suitable for large-scale industrial production. Liver seed cells can be subjected to efficient amplification and albumin synthesis through three-dimensional culture on a microcarrier prepared on the basis of edible materials, so that the cell culture liver is close to a real animal liver in the aspect of nutrition.

The invention belongs to the cross technical field of nanomedicine, tumor treatment and immunotherapy, and particularly provides tumor mitochondria-targeted copper death selective induction nanoparticles, which are prepared by the following preparation method: S1, specifically coupling TPY and MHI through condensation reaction to obtain MTPY; s2, MTPY and copper ions are subjected to complexation, and an MTPY-Cu complex is obtained; and S3, the MTPY-Cu complex and albumin are subjected to self-assembly, and the MTPY-Cu-coated Alb nanoparticles are formed. The dosage of copper is only 1 / 1000 of that of free Cu < 2 + >, and equivalent immune regulation and killing effects can be achieved; primary tumors and distant tumor focuses can be inhibited, and lung metastasis is reduced; immune memory can be induced, and relapse can be prevented; the traditional Chinese medicine composition is combined with cis-platinum to remarkably enhance the curative effect and reverse the induced immunosuppression; the invention has the advantages of high biological safety and no obvious liver and kidney toxicity or hemolytic reaction.

The invention discloses a cat serum albumin combined nano antibody as well as a preparation method and application thereof, and relates to the technical field of nano antibody engineering. Wherein the amino acid sequence of the cat serum albumin binding nano antibody Nb1 is shown as SEQ.ID NO.1, and the nano antibody Nb1 not only has higher affinity to cat serum albumin, but also has better binding activity to mouse serum albumin; the polypeptide can be widely applied to the aspects of drug half-life period enhancement, tumor targeted therapy and imaging, immunodetection or diagnosis, recombinant albumin purification or enrichment and the like in the pet treatment process.

Provided herein are albumin binding proteins and methods of making and using thereof.

The invention provides a soil conditioner as well as a preparation method and application thereof. The soil conditioner provided by the invention is prepared from the following raw material components in parts by weight: 35 to 45 parts of straw biochar, 55 to 65 parts of biological excrement, 0.01 to 0.5 part of cellulose degradation compound bacteria, 1 to 5 parts of nano silicon dioxide and 3.5 to 4.5 parts of bovine serum albumin. The soil conditioner provided by the invention is good in composting quality, high in composting efficiency, environment-friendly and suitable for industrial production.

The invention discloses a method for enriching whey protein main monomers from cow milk. According to the method, whey protein subjected to membrane separation is taken as a raw material, interaction between main monomers of the whey protein and other milk proteins is weakened by adopting a pH conversion technology and an ultrasonic technology, mineral substances, lactose, bovine serum albumin and residual casein are removed through ion exchange chromatography, the main monomers of the whey protein are enriched, and the high-purity whey protein is obtained. And drying the alpha-lactalbumin and the beta-lactoglobulin to obtain a milk protein product rich in two main monomers of the whey protein, and the total content of the two monomers is up to 85% or above. According to the technology, cow milk serves as a raw material, efficient enrichment of two main monomers of whey protein can be achieved only through membrane separation, pH conversion, ultrasonic assistance and ion exchange chromatography, only pH needs to be adjusted in the separation process, other chemical reagents are not added, and the process is environmentally friendly, simple, easy to implement and suitable for large-scale processing; the prepared milk protein base material rich in the whey protein main monomer can be used as a main material and a base material of infant food, and is high in nutritional value and wide in application prospect.

The invention belongs to the technical field of nuclear medicine molecular diagnosis and treatment, and discloses a modified CAIX targeting cyclic peptide, a nuclide marker and application of the modified CAIX targeting cyclic peptide in tumor diagnosis and treatment. According to the CAIX targeted cyclic peptide, a rigid bifunctional chelating agent RESCA is introduced to replace a traditional chelating agent, and a sulfonated or alkylated amino acid connector is combined, so that the enzymolysis resistance and in-vivo stability of the probe are remarkably improved. By optimizing the structure of the connector, the lipophilicity is reduced, liver and gall metabolism and non-specific uptake are reduced, and the high uptake rate of tumor target tissues is maintained. The therapeutic nuclide marker of the cyclopeptide can be used for intratumoral irradiation therapy using DOTA or NOTA in combination with an alkylated amino acid linker or an albumin ligand. Experiments show that the marker has high radiochemical purity, excellent pharmacokinetic characteristics and low kidney retention, and is suitable for precise diagnosis and targeted therapy of CAIX high expression tumors such as kidney cancer, colorectal cancer and brain glioma.

Provided herein are albumin binding proteins and methods of making and using thereof.

The invention relates to the technical field of biomedicine, and discloses a human metapneumovirus culture method which comprises the following steps: a culture medium is prepared and put into a culture bottle, and the culture medium comprises a DMEM culture medium, glucose, sodium bicarbonate, an HEPES buffer solution, chondroitin sulfate and hyaluronic acid; host cells are selected; preparing a culture solution, wherein the culture solution comprises L-glutamine, bovine serum albumin, antibiotics and N-acetylcysteine; and inoculating the human metapneumovirus into the culture medium for adsorption and infection. The chondroitin sulfate and the hyaluronic acid are added into the culture medium, the chondroitin sulfate and the hyaluronic acid can be combined with glycosaminoglycans on the surfaces of the host cells to form a compound, interaction sites of viruses and the host cells are increased, then virus receptor expression on the surfaces of the host cells is enhanced, the cell membrane environment is optimized, and therefore the anti-tumor effect is achieved. Therefore, the attachment and entry efficiency of the hMPV is improved, and the rapid proliferation of the virus is obviously promoted.

The invention relates to the technical field of food, and provides a pea complete protein component and a preparation and identification method thereof.The preparation method of the complete protein comprises the steps that pea kernel powder and water are mixed and then subjected to first solid-liquid separation, and first clear liquid is obtained; adjusting the pH value of the first clear liquid to 5.8-6.2 to obtain a protein mixed liquid; adding lactic acid bacteria into the protein mixed solution, and standing for 1-3 hours at the temperature of 10 DEG C or below for layering; removing a bottom layer material of the layered material to obtain a residual material, and carrying out second solid-liquid separation on the residual material to collect a precipitate; performing first drying on the precipitate to obtain protein isolate; mixing the separated protein and water, and performing third solid-liquid separation to obtain a second clear liquid; and separating the second clear liquid through an ultrafiltration membrane, and performing secondary drying on the obtained percolate to obtain albumin, namely the complete protein. The method disclosed by the invention is green and environment-friendly, and the extracted complete protein is good in balance and meets nutritional requirements.

The invention relates to the technical field of stem cell injections, in particular to a human umbilical cord mesenchymal stem cell injection and a preparation method thereof. The cell cryoprotectant comprises the following components: a cell cryoprotectant and P6-generation human umbilical cord mesenchymal stem cells. By selecting P6-generation human umbilical cord mesenchymal stem cells, the capacity of secreting hepatocyte growth factors (HGF) can be improved; the cell cryoprotectant contains 6-9wt% of dimethyl sulfoxide and a certain amount of human serum albumin, can better protect cells and maintain the motility rate and biological functions of the cells, and is more beneficial to preparation of cryopreserved human umbilical cord mesenchymal stem cell injection. The P6-generation human umbilical cord mesenchymal stem cells are obtained by culturing a culture medium containing fetal calf serum, and the ability of the injection to repair liver injury can be improved.

The invention belongs to the technical field of biological detection, and discloses a kit and a method for quantitatively detecting residual quantity of host cell protein of pichia pastoris in order to solve the problems of limit of quantitation and low detection sensitivity of an existing detection method. According to the present invention, the empty plasmid without the recombinant human serum albumin gene is introduced into the pichia pastoris, and purification is performed by combining the Protein A chromatographic column and the host cell protein coupling affinity column, such that the prepared polyclonal antibody can be effectively used for the quantitative detection of the residual amount of the pichia pastoris host cell protein in the biological product; the kit formed by the polyclonal antibody has good accuracy and repeatability on the quantitative detection of the host cell protein residues in the pichia pastoris source biological products, the quantification limit is 0.2 ng / mL, and the kit provided by the invention can meet the quantitative detection of the host cell protein residues in all the pichia pastoris source biological products.

The present invention relates to a nanobody or antigen-binding fragment thereof that specifically binds to serum albumin, a derivative comprising the nanobody or the antigen-binding fragment thereof, a nucleic acid encoding the antibody or antigen-binding fragment thereof, a host cell comprising same, and related uses. Further, the present invention relates to therapeutic use of the antibody or the antigen-binding fragment thereof or the derivative.

The embodiment of the invention provides breast cancer targeting nanoparticles as well as a preparation method and application thereof. According to the embodiment of the invention, the breast cancer targeting nanoparticles are synthesized by preparing the pH sensitive polymer, carrying out co-coupling modification on the pH response polymer and the folic acid albumin nano-carrier and utilizing a controlled desolvation method; the breast cancer targeting nanoparticle is combined with a nano preparation passive targeting strategy, a tumor microenvironment stimulation response strategy and a folic acid active targeting strategy, the effects of an EPR effect, PEG shedding, charge overturning, folic acid ligand-receptor combination and the like are utilized, precise targeting and specific response of tumors are achieved, and targeted accumulation of drugs in a tumor microenvironment is comprehensively improved. Therefore, the breast cancer targeting nanoparticles prepared by the embodiment have the advantages of high targeting property, drug controlled release, improvement of solubility and absorptivity of indissolvable drugs and the like, so that the curative effect of the drugs can be enhanced, the toxic and side effects can be reduced, and the breast cancer targeting nanoparticles have wide application prospects in the field of anti-tumor treatment.

The invention provides a construction method and application of a blood sinus networked liver organ. According to the method, pluripotent stem cells are co-differentiated into HAND1 < + > mesoderm and endoderm, a front intestine rear endoderm containing KDR < + > mesoderm progenitor cells is generated, a liver endoderm with CD34 < + > / CD31 < + > endothelial progenitor cells is generated, and the blood sinus networked liver organ is formed. The liver organ has a functional blood sinus endothelial network penetrating through the liver cluster, and has the characteristic functions of low-density lipoprotein uptake, human serum albumin removal, mediated secretion of blood coagulation factor 8 and the like. The method provides a new-generation highly bionic research tool for liver disease mechanism analysis, drug screening and regeneration repair.

The invention discloses a nutritional composition for promoting neurodevelopment and application, and provides a nutritional composition for promoting neurodevelopment, which comprises the following necessary components: a sialylated substance component comprising one or more of N-acetylneuraminic acid, sialylated oligosaccharide, sialylated protein and sialylated lipid; the whey protein component comprises alpha-lactalbumin; and a peptide component from casein, including a hydrolysate of casein; the nutritional composition comprises a sialylated substance component, a whey protein component and a casein-derived peptide component, and the mass ratio of the sialylated substance component, the whey protein component and the casein-derived peptide component in the nutritional composition is (0.5-10): (300-1): (100-0.5). The nutritional composition provided by the invention can promote brain development and cognitive functions, and can significantly promote neurodevelopment by scientifically matching the sialylated substance component, the whey protein component and the peptide component from casein.

The invention belongs to the technical field of biological medicines, and particularly relates to application of poecilobdella manillensis in preparation of a medicine for treating renal injury. The invention provides a new application of poecilobdella manillensis in preparation of a medicine for treating renal injury, serum biochemical index detection proves that the poecilobdella manillensis can effectively reduce serum creatinine and blood urea nitrogen levels, increase serum albumin and total protein content, remarkably improve glomerular filtration function and protein metabolism function, and improve renal function. And the improvement degree of the traditional Chinese medicine composition is superior to that of a traditional Chinese medicine contrast. Although the curative effect is slightly poorer than that of a chemical drug group, the safety advantage is remarkable. The poecilobdella manillensis can comprehensively regulate renal function metabolism indexes, relieve kidney injury and avoid common side effects of chemical drugs, provides a new choice for clinical treatment of kidney injury, and is especially suitable for chronic kidney disease patients needing long-term treatment.

The invention provides a composite quality control liquid for an electrochemical multi-parameter detector. The composite quality control liquid comprises a buffer volume, a quality control substance and a stabilizer, the stabilizing agent comprises 5 to 50 g / L of bovine serum albumin, 0.1 to 10 g / L of EDTA-2Na (Ethylene Diamine Tetraacetic Acid), 10 to 100 g / L of a viscosity regulator, 0.3 to 25 g / L of a preservative and 5 to 20 g / L of a surfactant; the preparation method comprises the following steps: adding components of the stabilizer into a buffer system, preparing a preparation solution, preparing mother liquor of each quality control substance, proportionally mixing glucose mother liquor, uric acid mother liquor, beta-hydroxybutyric acid mother liquor and cholesterol sodium sulfate and triglyceride composite mother liquor according to the target concentration of the quality control solution, and fixing the volume by using the preparation solution to obtain the quality control liquid. And fully and uniformly mixing to obtain the composite quality control liquid. The composite quality control liquid can ensure that uric acid is not degraded, cholesterol triglyceride is not separated out, the formula is mild, and active substances in a detection system are not influenced.

The invention discloses a composite microecological preparation as well as a preparation method and application thereof. The compound microecological preparation disclosed by the invention comprises lactobacillus casei, lactobacillus plantarum and saccharomyces cerevisiae. The application effect of the composite microecological preparation in cow breeding is remarkable, the optimal addition amount of the composite microecological preparation is 100 mL / cow / day, the production performance of lactating cows can be improved, the milk yield is improved by 3.30 kg / cow / day, the milk yield of 4% standard milk is improved by 3.78 kg / cow / day, the total protein is improved by 15.9%, the albumin is improved by 14.7%, the globulin is improved by 16.5%, and the somatic cell number is reduced by 52.6%; and pollutant emission can be reduced, including 37.2% of ammonia emission reduction and 28.7% of carbon dioxide emission reduction.

Disclosed in present invention are a novel cleavable linker compound as shown in formula (1) for a prodrug delivery system, and a pharmaceutically acceptable salt, solvate, polymorph or isomer thereof. Further disclosed are a prodrug compound as shown in formula (J) and formula (L) formed by covalently bonding an albumin or antibody to a camptothecin derivative by means of a novel linker compound, and a pharmaceutically acceptable salt, solvate, polymorph or isomer thereof. Further disclosed are a synthesis method for the compound, and a preparation method for the compound in an enzyme release prodrug system. Further disclosed are a use of the compound in preparation of an anti-tumor drug, and a use of the compound in preparation of an albumin-bound anti-tumor drug or antibody-drug conjugate. The present invention innovatively proposes a structure in which a trigger fragment in the compound is linked to the novel linker by means of p-hydroxyl or para-amino, an active drug is linked to the novel linker by means of the hydroxyl part of the active drug, and a modified fragment is linked to the novel linker by means of an amino group.

Provided are an anti-albumin antibody or an antigen-binding fragment thereof and the use thereof, and an anti-albumin nanobody with improved affinity or an antigen-binding fragment thereof. The anti-albumin antibodies or antigen-binding fragments thereof can bind to albumins from different species with high affinity. In addition, the anti-albumin antibody can be linked to a bioactive effector molecule to form a fusion construct without affecting the activity of the bioactive effector molecule.

The invention relates to a double-framework self-developing embolism system based on iodine modified PNIPAM gel and cross-linked albumin microspheres, which simulates a brick wall-concrete effect through a microsphere-gel composite structure and synergistically improves the embolism strength. A novel TACE treatment system is designed and constructed, multiple effects of tracing, embolism, hypoxia enhanced chemotherapy and tumor microenvironment improvement are achieved through the self-developing temperature-sensitive gel-drug carrying microspheres, and the anti-tumor effect is cooperatively improved.

The present disclosure provides, among other things, single domain antibodies that bind to human serum albumin. Pharmaceutical compositions comprising the single domain antibodies disclosed herein are provided.

The invention discloses a screening method of allergen substances in a carthamin yellow injection, a verification method of the allergen substances in the carthamin yellow injection, a detection method of the allergen substances in the carthamin yellow injection and a production process of the carthamin yellow injection. The screening method of the allergen substances in the carthamin yellow injection comprises the following steps: S1, extracting components in the carthamin yellow injection; s2, separating and screening components in the extract by adopting a high performance liquid chromatography-ultraviolet detection-mass spectrum and human serum albumin combined detection technology; and S3, screening out a substance which can be combined with the HSA, and determining the substance as a sensitization hapten substance which has sensitization potential. The invention aims to detect that the 6-hydroxykaempferol-3-rutin-6-glucoside has HAS binding characteristic, hapten attribute and sensitization potential, and can provide basis for process improvement of carthamin yellow injection and reduce the sensitization risk in clinical application.

The invention discloses a double-channel gold-copper-doped graphene electrochemical immunosensor as well as a preparation method and application thereof. The double-channel gold-copper-doped graphene electrochemical immunosensor comprises a gold / gold-copper co-doped vertical graphene electrode, and bovine serum albumin and an antibody which are incubated on the gold / gold-copper co-doped vertical graphene electrode, the gold / gold-copper co-doped vertical graphene electrode comprises a gold-copper co-doped vertical graphene electrode and gold nanoparticles loaded on the surface of the gold-copper co-doped vertical graphene electrode, and the gold-copper co-doped vertical graphene electrode comprises a substrate and a gold-copper co-doped vertical graphene nanosheet grown on the surface of the substrate. The sensor capable of sensitively detecting the concentration of the carcinoembryonic antigen secreted by the tumor cells is prepared, an immunoreaction detection system is built, a reliable industrial signal amplification platform is provided for early diagnosis of tumors, and a new strategy is developed for research of tumor cytology.