838 results about "Hormone" patented technology

The invention discloses a fresh commodity after-ripening regulation and control planning method oriented to maturity grading, and relates to the technical field of food science and biologication.The method comprises the steps that physiological parameters of fresh commodities are collected in real time based on a multi-modal sensor, and the physiological parameters comprise the epidermis pigment content, the ethylene release rate, the fruit hardness and the respiration intensity; the collected physiological parameters are input into a multi-objective optimization model based on deep learning, the model fuses a metabolic kinetic equation and an LSTM time sequence prediction algorithm, and a regulation and control parameter combination adaptive to the current mature stage is output; according to the regulation and control parameter combination, the temperature, the relative humidity and the O2 / CO2 concentration gradient of the controlled atmosphere storage equipment are synchronously regulated through a distributed control system, and a plant hormone antagonist is injected through an ultrasonic atomization device; and constructing a supply chain collaborative network based on a block chain technology, binding a grading result with logistics path planning, and adjusting a temperature control strategy and a shelf life countdown parameter of the cold chain transport vehicle in real time through an edge computing node.

The invention relates to the technical field of blood pressure dynamic monitoring systems, and provides an artificial intelligence-based blood pressure dynamic monitoring and risk assessment system, which comprises a patient terminal, a doctor terminal, a data center terminal, a local anomaly detection terminal and a strategy generation terminal, the patient terminal is used for collecting blood pressure, body position change and endocrine hormone fluctuation data of a patient in real time; the data center terminal is used for fusing, storing and processing the data uploaded by the patient terminal and performing overall risk assessment; the local anomaly detection terminal is used for collecting local blood flow rate and pulse wave conduction velocity data and carrying out local anomaly detection index analysis; the strategy generation terminal is used for generating strategy information according to the overall risk assessment result and the local anomaly detection index; and the doctor terminal is used for receiving and displaying the overall risk assessment result and the local anomaly detection index, and receiving and executing the strategy information. According to the invention, the comprehensiveness and timeliness of hypertension risk prediction and intervention are improved.

The invention relates to the technical field of biology, in particular to application of a rice OsPBL8 gene in enhancing resistance of rice to magnaporthe oryzae. The rice OsPBL8 gene is a rice endogenous gene participating in the processes of plant growth and development, abiotic stress response, hormone signal channel regulation and control and the like. The invention provides application of the OsPBL8 gene in the field of disease resistance, the OsPBL8 gene is overexpressed through gene editing, and the expression quantity of receptor-like cytoplasm kinase OsPBL8 in plants is increased, so that a plurality of signal channels are regulated and controlled, and the germ resistance of the plants is synergistically improved. By adjusting key hub nodes of plant targeted immune signal transduction, more lasting broad-spectrum disease resistance can be provided, good universality is achieved, disease resistance and growth can be balanced, good practicability is achieved, and improvement of disease resistance of various rice varieties is facilitated.

The invention belongs to the technical field of biological agents, and particularly relates to ginsenoside variant flavobacterium DG-16 and application thereof in relieving continuous cropping obstacles of angelica sinensis and improving the quality of the angelica sinensis. According to the ginsenoside variant flavobacterium DG-16 disclosed by the invention, the growth of angelica sinensis is promoted by increasing the contents of jasmonic acid and cytokinin hormones, reducing the contents of gibberellin and salicylic acid hormones and promoting the circulation and utilization of nitrogen; in addition, the flavobacterium ginsenoside variant DG-16 can significantly increase the chlorophyll content, is beneficial to photosynthesis of the angelica sinensis, effectively changes the physiological and biochemical states of the angelica sinensis, can resist root rot of the angelica sinensis so as to relieve successive cropping obstacles and improve the quality, and has a significant effect.

The invention relates to the field of medical image processing, and discloses an AI auxiliary screening method based on a mammary gland molybdenum target image, and the method comprises the steps: obtaining time sequence image data containing a historical image and current hormone cycle phase information; predicting a basic evolution state representing the long-term trend of the organization through a long-term evolution network; predicting a physiological modulation vector representing a short-term physiological change through a physiological cycle network; generating an anti-fact image in combination with the basic evolution state and the physiological modulation vector; and carrying out pixel-level subtraction on the current real mammary gland molybdenum target image and the anti-fact image to generate an evolution deviation graph. The system comprises a data acquisition and registration module, a basic evolution state prediction module, a physiological modulation vector prediction module, an anti-fact image generation module and an evolution deviation graph generation module, and is used for executing the method. According to the method, physiological change and pathological evolution are effectively separated through decoupling modeling, and the accuracy of early abnormal evolution recognition is improved.

The invention provides an application of a lactobacillus paracasei IOB413 metagen in prevention of helicobacter pylori infection, and relates to the technical field of microorganisms. The lactobacillus paracasei IOB413 metaplasm can competitively occupy gastric epithelial cell adhesion sites to directly block the initial colonization of helicobacter pylori, so that infection preposed interception is realized, and the lactobacillus paracasei IOB413 metaplasm is suitable for early prevention of high-incidence people. The compound has dual mechanisms of site blocking and urease inhibition, and can cooperate with antibiotics to improve the eradication rate and reduce the drug resistance. Animal experiments show that the traditional Chinese medicine composition can reduce gastric mucosa proinflammatory factors in a dose-dependent manner, improve the activity of antioxidant enzymes, reduce lipid peroxidation products, relieve mucosa lesion, promote repair and reduce the risk of complications; moreover, gastrointestinal hormone disorder can be reversed, the digestive function is maintained, and related discomfort is relieved.

The invention relates to a compound according to general formula (I), which acts as a bradykinin (BK) B2 receptor antagonist; to a pharmaceutical composition containing one or more of the compound(s) of the invention; to a combination preparation containing at least one compound of the invention and at least one further active pharmaceutical ingredient; and to said compound(s) for use as in a method of treating a skin disorder; eye disease; ear disease; mouth, throat and respiratory disease; gastrointestinal disease; liver, gallbladder and pancreatic disease; urinary tract and kidney disease; disease of male genitale organs and female genitale organs; disease of the hormone system; metabolic disease; cardiovascular disease; blood disease; lymphatic disease; disorder of the central nervous system; brain disorder; musculoskeletal system disease; allergy disorder; pain; infectious disease; inflammatory disorder; injury; immunology disorder; cancer; hereditary disease; or edema.

The present invention provides a production method of a thienopyrimidine derivative or a salt thereof which has a gonadotropin releasing hormone (GnRH) antagonistic action with high quality in high yield. The present invention provides a method of producing a thienopyrimidine derivative, which comprises reacting 6-(4-aminophenyl)-1-(2,6-difluorobenzyl)-5-dimethylaminomethyl-3-(6-methoxypyridazin-3-yl) thieno[2,3-d]pyrimidine-2,4 (1H,3H)-dione or salt thereof, 1,1′-carbonyldiimidazole or a salt thereof and methoxyamine or a salt thereof, and the like.

The invention discloses a composition containing D-chiro-inositol as well as a preparation method and application of the composition. The composition comprises D-chiro-inositol, Myo-inositol and microcapsule powder, wherein the wall material of the microcapsule powder consists of anhydride modified edible protein and a polysaccharide compound; the edible protein is selected from at least one of spirulina protein, quinoa protein, whey protein and soybean protein; the polysaccharide compound comprises at least one of fucoidin and larch arabinogalactan. Active components are also embedded in the microcapsule powder, and comprise at least one of Omega-3, coenzyme Q10, astaxanthin and vitamin. According to the composition, the stability and bioavailability of fat-soluble active ingredients are improved through microencapsulation, and the composition has a synergistic effect with components such as D-chiro-inositol and Myoinositol, so that insulin resistance is effectively improved, female hormone balance is adjusted, and polycystic ovarian syndrome is improved. The process is simple, is suitable for large-scale production, and can be widely applied to dietary supplements or functional foods.

The invention provides a cyprinus carpio sex determining gene ccMD and application thereof in cyprinus carpio sex determination or sex control, the full length of the cyprinus carpio sex determining gene ccMD is as shown in SEQ ID NO.1, the CDS sequence is as shown in SEQ ID NO.2, and the protein coded by the gene is as shown in SEQ ID NO.3. The cyprinus carpio sex determining gene ccMD can be applied to cyprinus carpio sex determination or sex control. Specific primers are designed according to the gene sequence characteristics, and XX, XY and YY carps can be rapidly and accurately identified. The gene is taken as a target gene, a gene editing technology and a gene transfer technology are adopted, hormone treatment is not needed, and a genetically male carp and a physiologically female carp or a genetically female carp and a physiologically male carp can be rapidly obtained.

The invention discloses a health-care food for delaying ovarian aging, which belongs to the technical field of health-care foods and comprises a composition for improving the levels of anti-mullerian test tube hormone and superoxide dismutase and functional auxiliary materials, the composition for improving the anti-muller test tube hormone and superoxide dismutase level is prepared from the following components in parts by weight: 2 to 5 parts of pyrroloquinoline quinone PQQ, 30 to 80 parts of cranberry powder and 40 to 100 parts of an isoflavone extract, and the content of the pyrroloquinoline quinone PQQ is 2 to 5 parts, the content of the cranberry powder is 30 to 80 parts, and the content of the isoflavone extract is 40 to 100 parts. The pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract in a specific formula have the effect of synergistically improving the levels of anti-muller test tube hormone and superoxide dismutase, and the health food prepared from the pyrroloquinoline quinone PQQ, the cranberry powder and the isoflavone extract can improve sleep, improve urinary tract infection of middle-aged women, delay ovarian aging and the like.

Disclosed are devices and methods for providing local administration of a sex hormone composition in a mammalian body to promote bone remodeling. The device includes an implantable structure configured for placement at a target intraosseous and / or periosteal site in the mammalian body and an effective amount of a sex hormone composition associated with the implantable structure. The sex hormone composition comprises both an estrogen and an androgen.

The present invention relates to methods of treating pleasant sensation loss in a human subject by administering a SIRT6 activator. The invention also provides methods of treating a pleasant sensation loss in a human female subject by administering a SIRT6 activator, wherein the female subject can be recognized by, for example, the presence of two X chromosomes or circulating a female hormone.

The invention discloses a pharmaceutical composition for delaying oocyte senescence and application thereof, and relates to the technical field of biological medicine, and the pharmaceutical composition comprises alpha-ketoglutaric acid, a bicarbonate buffer solution, an energy metabolism substrate, polyvinyl alcohol, hormone, a growth factor and insulin-transferrin-selenium. The pharmaceutical composition for delaying oocyte aging disclosed by the invention is beneficial to improving the maturation rate of oocytes, reducing the cytoplasm fragmentation rate and the cleavage fragmentation rate, and improving the cleavage rate and the blastocyst rate; according to the invention, the content of beta-galactosidase is reduced, the function of mitochondria in the aging process after ovulation of the mature oocyte is improved, the membrane potential of the mitochondria in the aging process after ovulation of the mature oocyte is stabilized, the activity of the mitochondria is improved, and the normalization of RNA methylation modification in the aging process after ovulation of the mature oocyte is promoted.

A method and apparatus for treatment of heart failure by increasing secretion of endogenous naturetic hormones ANP and BNP such as by stimulation of the heart atria. Heart pacing is done at an atrial contraction rate that is increased and can be higher than the ventricular contraction rate. Pacing may include mechanical distension of the right atrial appendage. An implantable device is used to periodically cyclically stretch the walls of the appendage with an implanted balloon.

The invention relates to the technical field of medical instrument detection, in particular to gestational hypertension related hormone rapid detection equipment which comprises a gestational hypertension monitor body, and a cleaning module is connected to the outer portion of the gestational hypertension monitor body in a hooked mode; the cleaning module comprises a mounting structure, a driving mechanism connected with the mounting structure and a water spraying mechanism fixed to the mounting structure and used in cooperation with the driving mechanism. The driving mechanism comprises a connecting shaft connected with the mounting structure, a sliding seat arranged outside the mounting structure in a sliding manner, and a swinging structure mounted between the connecting shaft and the sliding seat; the water spraying mechanism comprises a liquid storage tank fixed to the outer surface of the mounting structure, a water delivery pump and a pressure adjusting pipe, the water delivery pump communicates with the liquid storage tank and the pressure adjusting pipe through water delivery pipes, and an arc-shaped abutting seat abutting against the water delivery pump is mounted on the outer surface of the sliding seat. The device has the advantages of high efficiency, automation, excellent cleaning effect, high adjusting flexibility, compact and stable structure, energy conservation, environment friendliness, easiness in maintenance and operation and the like.

Disclosed are compounds that are thyroid stimulating hormone receptor antagonists, and methods useful for preventing or treating a thyroid disease.

The invention relates to the field of pituitary tumor monitoring, and discloses a pituitary tumor hormone level dynamic monitoring device, which comprises a multi-modal data acquisition and integration module, a detection analysis module, a data processing and analysis module, a clinical decision support module and a remote interaction and management module, the multi-modal data acquisition and integration module is electrically connected with the laboratory data interface, the multi-modal data acquisition and integration module is in communication connection with the minimally invasive implantable sensor and the dynamic monitoring sensor, and the detection and analysis module comprises a hypersensitive detection module and a hormone dynamic analysis module. And the data processing and analysis module comprises an edge calculation module and a cloud big data analysis module. The hormone level is accurately monitored through multi-modal acquisition and super-sensitive detection, data is efficiently processed through edge and cloud computing, decision is optimized through the clinical decision module, space-time limitation is broken through the remote interaction module, and quality improvement and efficiency improvement of medical services are assisted.

The invention provides a method for inducing test tube tuberous roots under dahlia pinnata tissue culture conditions, which belongs to the technical field of plant tissue culture and comprises the following steps: step 1, obtaining sterile explants; step 2, primary culture: removing a color-changing part on the wound surface of the to-be-inoculated explant obtained in the step 1, and then inoculating the to-be-inoculated explant into a primary culture medium for culture to obtain a sterile test-tube plantlet; step 3, subculture multiplication culture: cutting the seedlings into cluster blocks, and inoculating the cluster blocks into a subculture multiplication culture medium to obtain multiplication test-tube seedlings; step 4, rooting culture; performing illumination culture in a rooting culture medium for 4 weeks to obtain rooted test-tube plantlets; 5, test tube root tuber induction: inoculating the rooted test tube plantlet into a root tuber induction culture medium for illumination culture for 4 weeks to obtain dahlia pinnata test tube sterile root tubers; through segmented induction and addition of different exogenous hormones, the breeding efficiency of dahlia pinnata can be effectively improved, and the breeding period is shortened.

The invention relates to a breast cancer dynamic risk assessment method based on an adaptive model, and aims to improve the accuracy of early screening and risk prediction of breast cancer. The method comprises the following steps that firstly, clinical data of the unresected side mammary gland of an estrogen receptor positive breast cancer patient are obtained, and the data comprise the estrogen receptor state, the hormone treatment duration and mammary gland density classification; selecting an imaging detection mode according to the mammary gland density grading and the estrogen receptor state, and obtaining a calcification focus image of the target mammary gland; performing multi-parameter quantitative analysis on the image, and extracting parameters such as hydroxyapatite crystal proportion, calcification edge sharpness index and the like; and carrying out risk value calculation by using the trained adaptive risk assessment model, comparing the risk value with a preset risk grading threshold, and finally generating an assessment report based on a risk grade. According to the method, accurate dynamic risk assessment can be provided for the breast cancer patient, and personalized health management and intervention decision are supported.

The invention relates to the technical field of biology, and provides a genetic marker for children nephrotic syndrome genetic risk assessment. The invention relates to genetic detection and application of hormone sensitive nephrotic syndrome (pSSNS) of children. Nine risk sites, including new sites of 1q23.1, 1p36.13, 5p13.2, 10q21.3, 10q24.1 and the like, highly related to diseases are found by integrating whole genome association research (GWAS) data, combining Meta analysis and a conjugate false discovery rate (conjFDR) method and taking genetic information of IgA nephropathy as assistance. Research results show that genes near the loci have differential expression in pSSNS and IgAN patients, which prompts that the genes play an important role in the occurrence and development of diseases. The invention provides a molecular detection method based on the risk site, which can be used for risk assessment, auxiliary diagnosis and prognosis prediction of children's nephropathy. Meanwhile, the invention provides potential application values of the loci and related genes thereof in individualized medication and targeted therapy.

The invention discloses a cassia seed antibacterial liquid for eyes and a preparation method thereof. The semen cassiae antibacterial liquid for the eyes is prepared from the following components in percentage by mass: 2.0 to 3.0 percent of semen cassiae extract, 2.0 to 3.0 percent of magnolia flower leaf extract, 2.0 to 3.0 percent of flos chrysanthemi indici extract, 2.0 to 3.0 percent of radix gentianae extract, 2.0 to 3.0 percent of flos eriocauli extract, 1.0 to 2.0 percent of radix ginseng extract, 2.0 to 3.0 percent of rhizoma polygonati extract, 1.0 to 2.0 percent of radix codonopsis extract and the balance of water. The traditional Chinese medicine composition has the beneficial effects that various eyesight-improving traditional Chinese medicine components are adopted, the traditional Chinese medicine composition does not contain any artificial hormone and antibiotic, has no toxic or side effect, has the effects of clearing liver and improving eyesight, resisting bacteria and inflammation, and improving retina microcirculation and blood flow volume, and is matched with chlorhexidine acetate, so that the traditional Chinese medicine composition has the effects of clearing liver and improving eyesight, resisting bacteria and inflammation, and improving retina microcirculation and blood flow volume. The eye drops have the advantages that the eye drops have strong inhibition effects on candida albicans, escherichia coli and staphylococcus aureus, the components of the eye drops do not contain any irritant component, irritation influence on eyes is avoided, the preparation method is simple, and the eye drops can be easily applied to large-scale production and processing processes.

The invention discloses a silent pituitary ACTH cytoma identification method. The method comprises the following steps: determining a pituitary neuroendocrine tumor through imaging examination; a serum marker SAA (serum amyloid protein A) index is obtained through blood detection; constructing a silent pituitary ACTH cytoma recognition model by combining serum marker SAA indexes, age, gender, clinical symptoms and various hormone variable characteristics of pituitary; and identifying and judging the silent pituitary ACTH cytoma according to the identification model. The serum SAA is used as an index for identifying the silent pituitary ACTH cytoma, and the serum SAA is used as a mature serum detection index in clinical application, so that a doctor can accurately identify a high-risk subtype of the silent pituitary ACTH cytoma which is a pituitary neuroendocrine tumor, and can adjust an operation strategy according to factors such as invasiveness and recurrence risk of the tumor; therefore, the operation effect is improved, and patient prognosis is improved.

The invention belongs to the technical field of biological medicine, and particularly relates to a reagent for improving ovarian development and a preparation method and application thereof. The reagent for improving ovarian development is prepared by mixing the following raw materials in parts by weight: 20-30 parts of N-acetylcysteine; 5 to 10 parts of tranexamic acid; 3 to 8 parts of creatine; and 3000 parts of water. According to the reagent, the mRNA expression of PKD1P6 and follicle stimulating hormone receptors (FSHR) in ovarian tissues is up-regulated, the activity and functions of cumulus granular cells are enhanced, and apoptosis of the cumulus granular cells is inhibited, so that the effects of effectively promoting follicle development and inhibiting the apoptosis of the cumulus granular cells are achieved.

The invention relates to the technical field of medical treatment, and particularly discloses dihydroartemisinin-loaded nano-hydrogel, which is prepared from the following components: 5 to 20 percent of gelatin methacryloyl, 5 to 15 percent of nano-hydroxyapatite and 0.5 to 2 percent of dihydroartemisinin, according to the invention, through the constructed Ge MA-nHA-DHA functionalized hydrogel system, the local concentration and bioavailability of dihydroartemisinin are remarkably improved, and the repair and regeneration of bone tissues are promoted. Research results show that the system can effectively inhibit inflammatory response, promote proliferation and differentiation of osteoblasts, improve the structure and function of bone tissues, slow down further collapse of femoral heads and finally improve the living quality of patients. In addition, the biological material shows good biocompatibility and appropriate drug release characteristics when applied in vivo, and a novel effective means is provided for treatment of steroid-induced femoral head necrosis.

The invention discloses application of a SmCYP82D47 gene in improvement of eggplant bacterial wilt resistance, and belongs to the technical field of molecular biology. 1, and the nucleotide sequence of the gene is as shown in SEQ ID NO. 2. On the basis of positioning an eggplant bacterial wilt resistance linkage site by GWAS, the eggplant bacterial wilt resistance candidate gene SmCYP82D47 is screened through bioinformatics analysis such as gene sequence analysis and haplotype analysis; the tissue expression specificity, hormone response expression characteristics and the like of candidate genes are analyzed; by silencing candidate genes in a resistant material S027 and overexpressing the candidate genes in a susceptible material S054, the disease resistance conditions of a silent plant and a wild type plant as well as the wild type plant and an overexpressed plant are observed, so that the accuracy of a positioning result can be verified, and the effect of the candidate genes in regulating and controlling the bacterial wilt resistance of the eggplant is determined; important theoretical basis and technical means can be provided for eggplant disease-resistant molecular breeding.

The application discloses application of glycyrrhetinic acid as a feed additive to improve the egg laying rate of laying hens. In the laying hen diet of the application, only glycyrrhetinic acid is added, and no chemical synthetic drugs and hormone substances are added, so that the safety of chicken and egg products is guaranteed from the source. The application fully considers the feed cost in application and feed preparation problems, selects glycyrrhetinic acid with low cost, fully plays the medicinal value of traditional Chinese medicinal materials, and the glycyrrhetinic acid is a natural plant extract, has no toxic side effects on laying hens, meanwhile, the preparation and purification method of the glycyrrhetinic acid is simple, the market price is low, and the glycyrrhetinic acid has the advantages of large-scale application.

The invention discloses a lactobacillus acidophilus FMM01 with the effects of improving the contractility of pelvic floor muscles and repairing mucous membranes and application of the lactobacillus acidophilus FMM01, the lactobacillus acidophilus FMM01 is preserved in the China Center for Type Culture Collection, the preservation date is January 9, 2024, and the preservation number is CCTCC NO: M2024052. The lactobacillus acidophilus FMM01 has the advantages of self-adaption, high acid resistance, strong mucous membrane adhesiveness and no irritation to vaginal mucosa. The lyophilized powder is high in activity and excellent in stability; experimental results show that the lactobacillus acidophilus FMM01 can effectively inhibit bacteria, and especially can effectively inhibit enterobacter cloacae, vibrio parahaemolyticus and / or candida albicans. Besides, the lactobacillus acidophilus FMM01 can improve vaginal relaxation, improve pelvic floor muscle contractility and / or recover vaginal mucosa functions, can promote recovery of healthy florae after mixed vaginitis antibiotic / hormone treatment, improves the treatment effect of mixed vaginitis, and remarkably reduces the recurrence rate of mixed vaginitis.

This invention discloses a traditional Chinese medicine composition for treating male infertility due to liver stagnation and kidney deficiency, its preparation method, and its application, belonging to the fields of traditional Chinese medicine pharmaceutical technology and andrological reproductive health technology. The composition is formulated with Bupleurum chinense, Tribulus terrestris, Cynomorium songaricum, Cuscuta chinensis, Cornus officinalis, and Epimedium brevicornu in specific weight proportions, following the principles of soothing the liver and relieving stagnation, tonifying the kidney and replenishing essence, and unblocking the meridians to treat impotence. This invention employs an alcohol-water dual-phase fractional extraction process, and can be prepared into various dosage forms such as granules, capsules, and tablets. Pharmacological experiments have confirmed that the composition of this invention can increase sperm density and progressively motile sperm rate, improve penile tissue nitric oxide synthase (NOS) activity, regulate reproductive hormones, and reduce testicular oxidative damage; serum pharmacochemistry and metabolomics have clarified the active components entering the bloodstream and their pathways of action. This invention features a refined formulation, abundant medicinal resources, and simple preparation, making it suitable for large-scale production and clinical application in treating male infertility due to liver stagnation and kidney deficiency.

The method comprises the following steps: (1) peeling seeds, disinfecting the peeled seeds with 75% ethanol and 10% NaClO, and inoculating the disinfected seeds to a hormone-free MS culture medium; (2) after the seeds germinate, cutting a hypocotyl which grows robustly, and cutting the hypocotyl into sections of 1cm as explants; (3) inoculating the hypocotyl to an induction medium (MS + 1.5 mg / L 6-BA + 1.5 mg / L 2, 4-D + 0.5 mg / L NAA + 30 g / L cane sugar + 7 g / L agar with pH of 6.2), and culturing for 30 days under the conditions of 25 + / -2 DEG C and illumination for 12 h / d to obtain a white green callus with a good state, and the induction rate reaches 71.1%; and (4) transferring the callus to a proliferation culture medium (MS + 1.5 mg / L 6-BA + 0.5 mg / L NAA + 30g / L sucrose + 7g / L agar, pH being 6.2), and continuously culturing for 30 days to realize expansion proliferation of the callus. The method is easy and convenient to operate and good in repeatability, and an effective way is provided for rapid propagation, germplasm preservation and secondary metabolite production of the rheum himalayana.