89 results about "Bilirubin" patented technology

The invention provides an adsorbent capable of simultaneously adsorbing bilirubin and cytokines and a preparation method and application thereof, and the preparation method comprises the following steps: carrying out suspension polymerization reaction on a styrene monomer and a polyvinyl cross-linking agent in a dispersion medium under the action of a continuous multi-component pore-foaming agent and an initiator to prepare polystyrene-based macroporous resin; carrying out chloromethylation reaction on the polystyrene-based macroporous resin to prepare chlorine balls; carrying out post-crosslinking reaction on the chloromethylated beads to prepare the adsorbent capable of adsorbing bilirubin and cytokines at the same time; the continuous multi-component pore-foaming agent comprises a first pore-foaming agent, a second pore-foaming agent and a third pore-foaming agent of which the solubility parameters are gradually increased in sequence; the temperature of the suspension polymerization reaction is adjusted according to the mass fraction of the polyvinyl cross-linking agent in the monomer mixture and the solubility parameter of the continuous multi-component pore-foaming agent, and the monomer mixture is composed of styrene monomers and the polyvinyl cross-linking agent. The bilirubin and cell factors can be adsorbed at the same time, and the safety of blood purification is improved.

The invention discloses a heme oxygenase mutant and application of the heme oxygenase mutant in preparation of bilirubin through multienzyme coupling, and belongs to the field of biological catalysis engineering. The forward mutant of the heme oxygenase is obtained through a directed evolution technology, and heme can be efficiently catalyzed to synthesize biliverdin; the bilirubin is synthesized by further introducing biliverdin reductase, coupling cytochrome P450 reductase and formate dehydrogenase, and coupling the two core enzymes with foreign assistant enzymes to construct recombinant escherichia coli to catalyze heme to synthesize bilirubin. In the system, NADPH does not need to be exogenously added, the reaction time is about 4 h, the yield of bilirubin reaches 2.10 g / L, accumulation of intermediate products is not observed in the catalysis process, and the highest yield and the shortest reaction time in bilirubin synthesis reported at home and abroad at present are achieved. Compared with the traditional pig bile extraction, the method disclosed by the invention breaks through the resource limitation, is simple to operate and high in yield, and realizes efficient and green synthesis of bilirubin.

The invention belongs to the technical field of medical apparatus and instruments, and discloses a mechanical intelligence-based incubator closed blue light irradiation data management system, which comprises a state cluster data module for collecting multi-source heterogeneous data, correcting clock skew between an incubator and a preset biosensor through a space-time alignment protocol, and sending the corrected clock skew to a server; dynamically adjusting the sampling rate of the multi-source heterogeneous data based on the infant physiological data in the multi-source heterogeneous data after time alignment, and outputting a state cluster data packet with a timestamp; the layered extraction module is used for constructing a three-level feature processing assembly line based on the state cluster data packet, and comprises a base layer for calculating a base statistical magnitude through a sliding window; the logic layer generates a temperature and light effect index and a sealing safety coefficient according to the basic statistics and in combination with the multi-source heterogeneous data; the decision-making layer establishes a treatment efficiency prediction matrix based on a temperature-light effect index and a sealing safety coefficient, and predicts a correlation trend of blue light irradiation and bilirubin metabolism; the safety and the curative effect stability of infant treatment are improved.

The invention provides a bilirubin adsorbing material as well as a preparation method and application thereof, and belongs to the technical field of blood perfusion materials. The bilirubin adsorption material is mainly prepared from chitosan quaternary ammonium salt, microfibrillated cellulose, a surfactant and a cross-linking agent, wherein the surfactant is one or more of lauryl methacrylate, Span 80 and Span 85. The structure of the bilirubin adsorption material is that a hydrophilic polysaccharide chain is stabilized in a pore wall shell, and a hydrophobic chain of a surfactant is repelled and assembled to a pore wall core. When the bilirubin adsorption material adsorbs albumin-bilirubin, the adsorption effect is better than that of MFC / QCS frozen gel, and the adsorption capacity and the adsorption rate are enhanced along with the increase of the concentration of albumin.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention relates to the technical field of biomedical detection, and discloses a portable urine component detection system and method based on a fluorescence sensor array. The system comprises a fluorescence sensor array, different fluorescence probes are used for detecting different trace components in urine, and fluorescence color gradient changes are generated through a probe-target object reaction, so that bilirubin, glucose and albumin in the urine are detected. The detection system comprises a micro-fluidic chip which integrates a capillary driving channel and a colorimetric reaction area and is used for sample transmission and reaction area positioning; an ultraviolet excitation light source and a multi-angle optical sensor are arranged in the camera obscura type color acquisition module, and the camera obscura type color acquisition module eliminates ambient light interference and captures fluorescence chromaticity signals; and the intelligent data processing system is integrated with an intelligent analysis end of the smart phone, establishes a quantitative relationship between chromaticity parameters (RGB and hue saturation) and the concentration of a target object through a machine learning model, and outputs a detection result in combination with an ambient light correction algorithm.

An eyeshade structure for bilirubin detection includes a body, a first detection module, and a second detection module. The body includes a bottom plate and a side wall. The side wall is connected to the bottom plate and forms an accommodating space with the bottom plate. The first detection module is arranged on the bottom plate. The second detection module is arranged on the bottom plate and is adjacent to the first detection module. The first detection module and the second detection module each include a circuit board, a light-emitting element, an inner photographing element, and an outer photographing element. The circuit board is connected to the bottom plate. The light-emitting element is electrically connected to the circuit board. The inner photographing element is electrically connected to the circuit board. The outer photographing element is electrically connected to the circuit board.

The invention relates to the technical field of medical monitoring equipment, in particular to an intelligent drainage monitoring system and method after a hepatotomy based on multi-modal sensing, and the system comprises a sensing data collection module, a signal processing module, a data fusion analysis module and an abnormity recognition early warning module. The sensing data acquisition module comprehensively acquires pressure, component and diffusion state data of drainage liquid by utilizing an optical fiber pressure sensor array, a micro spectral analysis device and a biological impedance sensor, the signal processing module converts sensing data into digital signals, and the data fusion analysis module performs fusion analysis on the drainage liquid based on the signals. According to the system, the content of bilirubin and hemoglobin in drainage liquid and the diffusion state of the bilirubin and hemoglobin in the abdominal cavity are recognized and comprehensively analyzed, the abnormity recognition and early warning module compares an analysis result with a preset normal index, the abnormal condition is determined, an early warning signal is generated, the intelligence and accuracy of drainage management are effectively improved, and the working efficiency is improved. Postoperative complications can be reduced, and the rehabilitation quality of a patient can be improved.

The application relates to the technical field of biological pharmacy, and discloses a method for preparing high-bionic bovine bezoar by using a 3D printing technology and application. The preparation raw material of the high-bionic bovine bezoar comprises the following components: fermented bovine bile, saturated calcium hydroxide, hydroxypropyl methyl cellulose, bilirubin, cholic acid, deoxycholic acid and taurine; wherein the fermented bovine bile is obtained by fermenting bovine bile by using escherichia coli. The high-bionic bovine bezoar prepared by using the preparation raw material to perform 3D printing can be comparable to natural bovine bezoar in terms of components, effects and appearance, the main pharmaceutical components can be adjusted according to actual requirements, the production cost of the bovine bezoar can be greatly reduced, and the demand of people for the bovine bezoar can be met.

This invention provides a process for extracting biliverdin hydrochloride through bio-fermentation and a method for synthesizing bilirubin. By studying the complex system of biliverdin obtained through microbial bacterial fermentation, a process for separating and purifying biliverdin was established, and the obtained biliverdin was further used to synthesize bilirubin. This process is simple to operate, uses common resins, employs solvents with low toxicity that are easily recovered and reused, utilizes low-energy membrane separation equipment for concentration, and uses conventional reaction reagents as reducing agents. The process has low operating costs, is environmentally friendly, and yields high-purity and stable-yield biliverdin and bilirubin products, demonstrating excellent prospects for industrial application.

The invention relates to the technical field of bilirubin extraction, in particular to a bilirubin extraction-based heating and cooling tank for biological pharmacy, which comprises a tank body, a stirring mechanism is arranged in the tank body, one end of the top of the tank body is communicated with an exhaust pipe, and a plurality of uniformly distributed exhaust holes are formed in the top end of the exhaust pipe. A shielding seat is fixedly mounted at the top of the exhaust pipe, the exhaust pipe is fixedly sleeved with a collecting box, a defoaming mechanism and a pushing mechanism are arranged in the exhaust pipe, and a defoaming agent spraying mechanism is arranged in the collecting box. The device has the beneficial effects that the defoaming mechanism is arranged in the exhaust pipe, when the foam amount exceeds the processing capacity of a defoaming paddle and is sprayed out from the exhaust pipe, the two defoaming plates in the defoaming mechanism pierce and eliminate the foam, and in the process of extracting bilirubin through pig bile in biological pharmacy, the defoaming effect is good. And the problem of bilirubin loss caused by the fact that foam sprayed out of the exhaust pipe carries part of bilirubin is avoided.

Disclosed herein are improved pluripotent stem cell-derived multi-zonal liver organoids, having two or more hepatocyte subpopulations, and methods of producing the same, by coculturing a first human liver organoid (HLO) with a bilirubin-treated second HLO, and contacting the co-cultured HLOs with bilirubin for a period of time sufficient for the co-cultured HLOs to self-assemble and fuse into interconnected dual organoids. The first HLO can be an ascorbateenriched HLO, such as a functional L-gulonolactone oxidase (GULO)-expressing HLO. In particular embodiments, the hepatocyte subpopulations can include zone 1 and zone 3 hepatocyte subpopulations, or zone 1, zone 2, and zone 3 hepatocyte subpopulations. Also disclosed are methods of studying or treating a liver-related disease or disorder using the described multi-zonal liver organoids.

The invention belongs to the technical field of biochemical pharmacy, and particularly relates to a compound bezoar composition, decoction piece type compound bezoar and a preparation method of the decoction piece type compound bezoar. The compound calculus bovis composition provided by the invention comprises 94-96% by mass of main agents and 8 kinds of calculus bovis intermediates, the mass content of the auxiliary agent is 4-6%, and the auxiliary agent is six commercially available finished product combined cholic acid. The bilirubin content of the bezoar intermediate used in the invention is increased by more than 30% and the yield is increased by more than 25% compared with the existing biochemical reaction process of oxgall. The DPPH free radical scavenging rate of the compound bezoar composition reaches 89.6% + / -2.1% and is increased by 25.7-36.9% compared with 58.3% + / -3.5% of a product prepared from ox bile, and through t inspection, p is smaller than 0.01 (n is equal to 5). Free cholic acid intervention is strictly prohibited, lithocholic acid addition is completely eradicated, and the composition has the advantages of being high in bilirubin content, high in oxidation resistance and high in biological activity and adapts to the physiological characteristics of the human body.

This invention discloses a single-herb bezoar preparation based on an ancient prescription; an enteric-coated bezoar soft capsule and its preparation method. The enteric-coated bezoar soft capsule consists of a bezoar dispersion and an enteric-coated soft capsule shell. A sand mill is used to prepare bezoar powder into micron-sized particles with a particle size ≤100μm. The micron-sized bezoar powder is fully dispersed in a dispersion medium, thereby improving the solubility of bezoar. The single-herb bezoar preparation can improve the problem of inconsistent bezoar content in compound bezoar preparations. The process of preparing the ≤100μm micron-sized bezoar dispersion uses non-metallic grinding equipment, avoiding the catalytic reaction of metal ions during the preparation of micron-sized powder. Low-temperature grinding is also used to avoid denaturation of the heat-sensitive active substances in bezoar, better preserving its biological activity. The enteric-coated formulation prevents the drug from being released in the stomach until it reaches the small intestine, avoiding the precipitation of bilirubin and other bile acids in the acidic environment of the stomach, which would otherwise lead to insoluble solids and poor solubility.

The invention discloses a platelet antibody detection kit and application thereof. Belongs to the technical field of detection kits. The kit comprises a microcolumn gel card, an enzyme-labeled secondary antibody, an enzyme-labeled secondary antibody diluent, a 20x washing solution, a developing solution, a fresh platelet treatment solution, a platelet buffer solution, a platelet preparation solution, a freeze-dried platelet reconstitution fluid, freeze-dried platelets and a negative control. The platelet antibody detection kit disclosed by the invention has the beneficial effects that the platelet antibody detection kit disclosed by the invention enables cross-matching of platelets to be possible; according to the invention, a micro-column gel immunoassay technology is combined with an enzyme immunolabeling technology, so that the limitation that only agglutination reaction can be carried out in a micro-column gel method is solved; according to the reaction card washing equipment disclosed by the invention, semi-automatic operation of micro-column gel method detection is realized; in the plasma, triglyceride is less than or equal to 5mg / mL, bilirubin is less than or equal to 20mg / dL, and hemoglobin is less than or equal to 5mg / mL, so that the result is not influenced; the kit has the advantages of good stability, long effective period, high sensitivity, strong specificity and simple operation.

A composition includes a compound of Formula 1, a solvate thereof, or a salt thereof. A method for treating an inflammatory disease includes administering the compound to a subject in need thereof. The compound of Formula 1 can protect cells and reduce inflammation by removing reactive oxygen species and suppressing inflammatory cytokines in a non-toxic manner.

The application belongs to the field of biological detection, and particularly relates to a technology and a reagent for detecting NAG by a VRA-NAG substrate method. The linear range of the NAG detection reagent provided by the application can reach (0, 500] U / L; within the range, when the anti-acid is less than or equal to 340 micromoles / L, urea is less than or equal to 42.9 millimoles / L, uric acid is less than or equal to 1.4 millimoles / L, albumin is less than or equal to 100 milligrams / dL, bilirubin is less than or equal to 342 micromoles / L, lactic acid is less than or equal to 6.6 millimoles / L, glucose is less than or equal to 55 millimoles / L, triglyceride is less than or equal to 37 millimoles / L, and oxalic acid is less than or equal to 81 micromoles / L, these components have no obvious interference on the test. Moreover, the relative standard deviation caused by the accelerated aging of the detection reagent for 10 days is not more than ±10%. Compared with the prior art, the kit provided by the application has high sensitivity, wide linear range, strong specificity and good stability, and is conducive to early detection and early treatment of kidney diseases in clinical practice.

The invention discloses a method for culturing calculus bovis by utilizing flora, which is characterized in that cattle gall bladders obtained by slaughtering are used as reaction containers, so that the animal welfare problem caused by living body surgery is completely avoided. A plurality of key steps of bilirubin generation, cholesterol conversion, matrix formation and the like are synergistically completed by adopting functional complementary synthetic flora, and the components of the generated product are closer to a complex system of natural calculus bovis. The breeding is carried out in a closed bioreactor with controllable parameters, uncontrollable factors such as individual cattle difference and feeding environment are eliminated, and the product quality is highly stable and uniform. Automatic monitoring and feedback control are achieved in the whole cultivation process, and technological parameters are accurate and adjustable. The used strains are all non-pathogenic or food-grade safe strains, and the whole process is sterile, free of exogenous pathogen pollution and antibiotic residue risks, and high in biological safety.

Disclosed herein are modified cyclodextrin compounds (e.g., compounds of Formula I, I-a, Lal, Lb, Lbl, Lc, or Lcl) and methods of their use (e.g. methods of sequestering bilirubin by administering said compounds).

The application belongs to the technical field of medical equipment, and provides a blood treatment system including a blood treatment assembly, an optical detection assembly and a control module. The blood treatment assembly uses a treatment liquid to treat blood of a target object to obtain waste liquid and treated blood, and the treated blood is transfused back to the target object. The optical detection assembly generates irradiation light of at least three wavelengths, wherein the irradiation light of the first wavelength is used for detecting bilirubin or free hemoglobin, the irradiation light of the second wavelength is used for detecting intracellular hemoglobin, and the irradiation light of the third wavelength is used for detecting the waste liquid. The optical detection assembly collects light information of emergent light of the at least three wavelengths. The control module obtains light change information corresponding to the at least three wavelengths according to the light information of the emergent light of the at least three wavelengths and the light information of the irradiation light of the at least three wavelengths. Whether the light change information corresponding to the at least three wavelengths satisfies a preset alarm condition is judged to determine whether to perform a hemolysis alarm or a blood leakage alarm, so that detection is distinguished.

The present application belongs to the technical field of medical apparatus and instruments, and discloses a warm box closed blue light irradiation data management system based on mechanical intelligence, which comprises: a state cluster data module, which collects multi-source heterogeneous data, corrects the clock deviation between the warm box and the preset biological sensor through a space-time alignment protocol, dynamically adjusts the multi-source heterogeneous data sampling rate based on the infant physiological data in the multi-source heterogeneous data after time alignment, and outputs a state cluster data packet with a time stamp; a hierarchical extraction module, which constructs a three-level feature processing pipeline based on the state cluster data packet, including a basic layer that calculates basic statistics through a sliding window; a logic layer that generates a temperature-light effect index and a sealing safety coefficient according to the basic statistics and in combination with the multi-source heterogeneous data; and a decision layer that establishes a treatment efficiency prediction matrix based on the temperature-light effect index and the sealing safety coefficient, and predicts the associated trend of blue light irradiation and bilirubin metabolism; the safety and curative effect stability of infant treatment are improved.

The invention discloses an image processing method and device for judging suspension red blood cell hemolysis. The method comprises the following steps: acquiring a blood image set; the blood image set comprises a plurality of blood images and corresponding label information; acquiring a blood parameter information set; the blood parameter information set comprises a plurality of blood parameters and corresponding label information; the blood parameters comprise hemoglobin concentration, reticulocyte concentration, indirect bilirubin concentration, lactic dehydrogenase concentration and globin concentration; utilizing the blood image set and the blood parameter information set to construct a blood hemolysis composite detection model; based on the blood hemolysis composite detection model, processing the collected blood image and blood parameters to obtain hemolysis detection result information; the hemolysis detection result information is used for representing erythrocyte hemolysis information of the blood corresponding to the collected blood image and blood parameters.

This invention discloses a bilirubin-modified dendritic nanomedicine carrier, its preparation method, and its applications, belonging to the field of pharmaceutical formulation technology. The bilirubin-modified dendritic nanomedicine carrier is synthesized by an amidation reaction between dendritic macromolecules and bilirubin. It exhibits relatively small particle size, good water solubility, high stability, and a large number of amine functional groups on its surface, making it suitable for delivering small-molecule hydrophobic drugs. This invention also discloses a nanomedicine composed of the aforementioned bilirubin-modified dendritic nanomedicine carrier and a small-molecule hydrophobic drug, with the small-molecule hydrophobic drug loaded within the hydrophobic cavity of the bilirubin-modified dendritic nanomedicine carrier. This nanomedicine exhibits extremely low cytotoxicity, good biocompatibility, and high antioxidant properties, showing broad application prospects in the preparation of drugs for the treatment and / or prevention of retinal vascular diseases.

Techniques are disclosed for measuring a concentration of an analyte in a subject based on reflectance spectra obtained from the subject. The spectra are obtained while and / or before and / or after photoreactions are induced in the subject, where the photoreactions change the reflectivity of the subject. The rate of change of the reflectivity is correlated with the concentration of the analyte.

The invention relates to the cross technical field of molecular biology and parasitology, and particularly discloses a giardia living body marking method based on fluorescent protein UnaG, which comprises the following steps: firstly, optimizing and synthesizing a UnaG gene sequence according to the codon use preference of giardia; then, constructing a marker plasmid pGT-UnaG-neo on a double expression framework containing an alpha2-tubulin promoter and a gdh promoter, and introducing the plasmid into the giardia giardia trophozoite through electroporation. And carrying out resistance screening in a culture medium containing geneticin to obtain an insect strain capable of stably integrating and expressing UnaG. Through bilirubin induction, efficient maturation and green fluorescence emission of the UnaG protein in giardia cytoplasm are realized. The stably transferred strain obtained by the method has no significant difference from a wild strain in the aspects of growth curve and survival rate, and UnaG and bilirubin have no obvious toxic effect. The limitation that traditional fluorescent protein is inactivated under the anaerobic condition is broken through, and a reliable tool is provided for dynamic imaging, functional research and drug screening of giardia and other anaerobic protozoa.

The invention belongs to the technical field of biological medicines, and relates to application of (+)-Sparteine (SPA) or pharmaceutically acceptable salts thereof in preparation of medicines for preventing, relieving or treating hepatic fibrosis. The invention proves that (+)-Sparteine can activate natural killer (NK) cells in a chronic inflammatory fibrosis environment and enhance adhesion and cytotoxic effects of the (+)-Sparteine on activated hepatic stellate cells (HSCs), so that serum biochemical indexes (AST, ALT and total bilirubin) of mice with hepatic fibrosis are remarkably improved, and collagen deposition of hepatic tissues is reduced. The invention provides a new strategy and a molecular target for the treatment of hepatic fibrosis.

1. The name of the design product: bilirubin adsorption column. 2. The use of the design product: for adsorbing bilirubin and bile acid in blood plasma. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application discloses a kind of polyether sulfone microspheres, preparation method and application, comprising the following steps: step 1: vinyl imidazole bromide salt, acrylic acid and azobisdimethylvaleronitrile are dissolved in solvent, stirring is carried out under nitrogen protection condition, fully reacts, precipitates, and is filtered to obtain polymer;Step 2: the polymer obtained in step 1 is dissolved in solvent to obtain polymer solution;Step 3: the polymer solution obtained in step 2 and polyether sulfone solution are blended, and polyether sulfone microspheres are obtained by electrospinning;The polyether sulfone microspheres obtained by the application have a diameter of 500-800 μm, have a loose porous structure, have a large specific surface area, and have good bilirubin adsorption effect.

The invention relates to the technical field of blood perfusion adsorbents, in particular to an MOF-based adsorbent, a preparation method thereof and application of the MOF-based adsorbent in blood perfusion. The preparation method comprises the following steps: adding MIL-101 (Fe)-NHH spherical particles into a polar solvent to prepare an MIL-101 (Fe)-NHH suspension; the preparation method comprises the following steps: respectively weighing carboxyl terminal short-chain polyethylene glycol, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide and N-hydroxysuccinimide, and adding into N, N-dimethylformamide to prepare a PEG-NHS (Polyethylene Glycol-N-Hydroxysuccinimide) active ester solution; the preparation method comprises the following steps: dropwise adding a PEG-NHS active ester solution into an MIL-101 (Fe)-NHS suspension under a stirring condition, reacting the obtained mixed solution at 60-120 DEG C for 6-24 hours under the protection of inert gas, cooling the obtained reaction solution to room temperature, centrifuging to obtain a precipitate, and sequentially washing, carrying out ultrasonic treatment and drying to obtain the MOF-based adsorbent. The MOF-based adsorbent prepared by the invention is an efficient bilirubin adsorbent with high specific surface area, adjustable aperture environment, good blood compatibility and green and safe preparation process.

The invention relates to the technical field of biology, and particularly discloses a construction method and application of a recombinant Escherichia coli strain with high yield of bilirubin, and the construction method of the strain comprises the following steps: knocking out a heme precursor efflux gene in Escherichia coli, co-expressing the following modules in the Escherichia coli, introducing a gene gdha for enhancing coenzyme factor circulation, and carrying out expression to obtain the recombinant Escherichia coli strain with high yield of bilirubin. According to the method disclosed by the invention, heme precursor exclusion genes are knocked out in escherichia coli, meanwhile, heme oxygenase and biliverdin reductase are modularly assembled by adopting a protein covalent coupling technology, and the yield of bilirubin is increased by increasing synthesis of coenzyme.