187 results about "Bilirubin" patented technology

The invention discloses a heme oxygenase mutant and bilirubin efficiently synthesized by a multienzyme coupling system of the heme oxygenase mutant, and belongs to the field of biological catalysis engineering. The invention provides a heme oxygenase mutant which is used for catalyzing heme to synthesize biliverdin. Coupling biliverdin reductase to catalyze biliverdin to synthesize bilirubin; cytochrome P450 reductase is introduced to provide electrons; removing competitive binding of the by-product to the substrate heme by carbon monoxide dehydrogenase; formate dehydrogenase is used for in-situ regeneration of coenzyme, and bilirubin is efficiently synthesized through a one-pot method. According to the heme oxygenase mutant, the two core enzymes are coupled with three external assistant enzymes, recombinant escherichia coli is constructed, heme is catalyzed, exogenous NADPH does not need to be added, and the yield of bilirubin within 4 h reaches 1611 mg / L and reaches the bilirubin synthesis level which is reported at present and has the highest yield and the shortest time. According to the technology, resource limitation is relieved, the synthesis method is easy to operate and high in yield, and efficient and green synthesis of bilirubin is achieved.

The invention provides an adsorbent capable of simultaneously adsorbing bilirubin and cytokines and a preparation method and application thereof, and the preparation method comprises the following steps: carrying out suspension polymerization reaction on a styrene monomer and a polyvinyl cross-linking agent in a dispersion medium under the action of a continuous multi-component pore-foaming agent and an initiator to prepare polystyrene-based macroporous resin; carrying out chloromethylation reaction on the polystyrene-based macroporous resin to prepare chlorine balls; carrying out post-crosslinking reaction on the chloromethylated beads to prepare the adsorbent capable of adsorbing bilirubin and cytokines at the same time; the continuous multi-component pore-foaming agent comprises a first pore-foaming agent, a second pore-foaming agent and a third pore-foaming agent of which the solubility parameters are gradually increased in sequence; the temperature of the suspension polymerization reaction is adjusted according to the mass fraction of the polyvinyl cross-linking agent in the monomer mixture and the solubility parameter of the continuous multi-component pore-foaming agent, and the monomer mixture is composed of styrene monomers and the polyvinyl cross-linking agent. The bilirubin and cell factors can be adsorbed at the same time, and the safety of blood purification is improved.

Wearable devices for measuring health indicators such as bilirubin are provided. The device includes a skin-facing side including a circuit board. The circuit board includes a spectrometer integrated circuit, at least one light emitting diode (LED) configured to direct light at the subject's skin where the LED is operably connected to a power source, at least one photodetector configured to receive light reflected from the subject's skin, and a microcontroller configured to perform spectral subtraction of said reflected light.

The invention belongs to but is not limited to the technical field of biomedicine, and discloses application of golden amide alcohol ester to preparation of a medicine for treating cholestasis. The in-vitro cell level verifies that the golden amide alcohol ester has relatively strong activity of activating FXR and CAR. The golden amide alcohol ester can relieve the cholestasis symptom of a cholestasis model mouse. The golden amide alcohol ester can obviously reduce the content of total bile acid and bilirubin in cells, relieve cholestasis and relieve hepatocyte damage. The golden amide alcohol ester can regulate and control the protein level of transporters and metabolic enzymes related to bile acid and bilirubin through double excitation of FXR and CAR, relieve cholestasis and achieve the purposes of benefiting gallbladder and removing jaundice.

The invention discloses a heme oxygenase mutant and application of the heme oxygenase mutant in preparation of bilirubin through multienzyme coupling, and belongs to the field of biological catalysis engineering. The forward mutant of the heme oxygenase is obtained through a directed evolution technology, and heme can be efficiently catalyzed to synthesize biliverdin; the bilirubin is synthesized by further introducing biliverdin reductase, coupling cytochrome P450 reductase and formate dehydrogenase, and coupling the two core enzymes with foreign assistant enzymes to construct recombinant escherichia coli to catalyze heme to synthesize bilirubin. In the system, NADPH does not need to be exogenously added, the reaction time is about 4 h, the yield of bilirubin reaches 2.10 g / L, accumulation of intermediate products is not observed in the catalysis process, and the highest yield and the shortest reaction time in bilirubin synthesis reported at home and abroad at present are achieved. Compared with the traditional pig bile extraction, the method disclosed by the invention breaks through the resource limitation, is simple to operate and high in yield, and realizes efficient and green synthesis of bilirubin.

A method of evaluating disposition and / or effect of a candidate compound in an in vitro culture and / or suspension to predict in vivo disposition and / or effect of the candidate compound, which can include the steps of providing a cell culture and / or suspension; exposing a candidate compound to the culture and / or suspension; exposing the culture and / or suspension to a media providing an in vivo relevant extracellular environment, such as a media comprising a component (such as a protein and / or other component, such as lipoproteins, bile acids, and endogenous compounds such as bilirubin) at a physiologic concentration or a concentration having characteristics, such as binding characteristics, similar to the physiological concentration; wherein any combination of any of the exposing steps can occur in any order or simultaneously; and evaluating in vitro disposition and / or effect of the compound to predict in vivo disposition and / or effect of the candidate compound.

The invention discloses hydrogel for repairing volumetric muscle defects and a preparation method of the hydrogel, and the hydrogel is prepared from oxidized dextran, an alcohol-polyamino acid-bilirubin polymer and 3, 3 '-dithio-bis (propionyl hydrazide) according to the weight ratio of (50-75): (4-20): 1. The alcohol-polyamino acid-bilirubin polymer is prepared from an alcohol-polyamino acid polymer and bilirubin in a weight ratio of (6-25): 1. The hydrogel prepared by the invention not only has good characteristics of pH / ROS responsiveness, relatively strong tissue adhesion, antibacterial property, oxidation resistance and relatively low cytotoxicity, but also can remarkably promote cell migration, proliferation, new angiogenesis and myoblast differentiation, promote recovery of muscle structures and motor functions after volumetric muscle defects, and improve the recovery rate of muscle tissues and motor functions after the volumetric muscle defects. The material is expected to become a novel bioactive material capable of promoting volume muscle defect repair, and has a good application prospect in clinical treatment of large-volume tissue defect regeneration.

The invention discloses a method for extracting bilirubin, cholic acid and chenodeoxycholic acid from chicken bile. The method comprises the steps of pretreatment, chicken bile treatment, hydrolysis reduction, chromatographic separation and the like. The method comprises the following steps: slicing frozen chicken gallbladder, heating, unfreezing and melting to denature protein into solid, then cooling, performing three-phase centrifugal degreasing and deproteinization, and adding three enzymes including biliverdin reductase, coenzyme I and glucose dehydrogenase to reduce free biliverdin and combined biliverdin, so as to obtain the biliverdin-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase-free biliverdin-glucose dehydrogenase. The method comprises the following steps: adding cholic acid and chenodeoxycholic acid into a container, adding gluconic acid hydrolase and bile salt hydrolase to hydrolyze biliverdin and bile acid, adding trypsin to hydrolyze impure protein, performing chromatography by using macroporous silica gel, and separating bilirubin, cholic acid and chenodeoxycholic acid by using different molecular space structures to obtain high-purity bilirubin, cholic acid and chenodeoxycholic acid. The method has the advantages of simple process, few types of used solvents, low toxicity, and facilitation of environmental protection and industrial production.

The invention provides a combined use of a hyaluronic acid-bilirubin conjugate and honokiol in preparation of a medicine for treating osteoarthritis. The invention provides a honokiol-loaded nanoparticle, which is characterized in that a hyaluronic acid-bilirubin conjugate and honokiol are used as raw materials to prepare a nanoparticle HKL-PNPs, and then the nanoparticle HKL-PNPs is modified by a mitochondrial targeting peptide to obtain the honokiol-loaded nanoparticle HKL-MPNPs. The invention also provides a bionic nanoparticle for treating bone joints. The bionic nanoparticle is prepared by wrapping the honokiol-loaded nanoparticle with an engineered cartilage cell membrane (ECM). According to the invention, cartilage cells with high expression of IL-1R2 are obtained through a gene editing method for the first time, and mitochondrial targeted nanoparticle cores loaded with Sirtuin-3 agonists are coated by cell membranes of the cells, so that the bionic nano preparation is obtained and is used for treating OA. The prepared bionic nano preparation not only can neutralize IL-1beta mediated inflammatory reaction, but also can recover Sirtuin-3 mediated mitochondrial function imbalance, so that the result of synergistic treatment of OA is achieved.

The invention discloses a preservation reagent, a preservation method and a detection method for fat-soluble vitamins D2, D3, A and E in peripheral blood. The preservation reagent comprises the following components: 1-15% of a PBS (Phosphate Buffer Solution) (v / v), 80-95% of a sodium chloride solution (v / v), 0.5-2.5% of Tris-HCl (v / v) and 0.5-2.5% of a stabilizer (w / v). According to the preservation reagent and the preservation method, fat-soluble vitamins D2, D3, A and E in the human whole blood sample can be stably preserved for 28 days at the temperature of 2-8 DEG C under the dark condition, preservation and transportation of the human whole blood sample are facilitated, and detection of the fat-soluble vitamins is facilitated. The precision and accuracy of detection can be improved, and interference of triglyceride, bilirubin and the like on detection of fat-soluble vitamins can be avoided.

The invention belongs to the technical field of medical apparatus and instruments, and discloses a mechanical intelligence-based incubator closed blue light irradiation data management system, which comprises a state cluster data module for collecting multi-source heterogeneous data, correcting clock skew between an incubator and a preset biosensor through a space-time alignment protocol, and sending the corrected clock skew to a server; dynamically adjusting the sampling rate of the multi-source heterogeneous data based on the infant physiological data in the multi-source heterogeneous data after time alignment, and outputting a state cluster data packet with a timestamp; the layered extraction module is used for constructing a three-level feature processing assembly line based on the state cluster data packet, and comprises a base layer for calculating a base statistical magnitude through a sliding window; the logic layer generates a temperature and light effect index and a sealing safety coefficient according to the basic statistics and in combination with the multi-source heterogeneous data; the decision-making layer establishes a treatment efficiency prediction matrix based on a temperature-light effect index and a sealing safety coefficient, and predicts a correlation trend of blue light irradiation and bilirubin metabolism; the safety and the curative effect stability of infant treatment are improved.

The invention provides a bilirubin adsorbing material as well as a preparation method and application thereof, and belongs to the technical field of blood perfusion materials. The bilirubin adsorption material is mainly prepared from chitosan quaternary ammonium salt, microfibrillated cellulose, a surfactant and a cross-linking agent, wherein the surfactant is one or more of lauryl methacrylate, Span 80 and Span 85. The structure of the bilirubin adsorption material is that a hydrophilic polysaccharide chain is stabilized in a pore wall shell, and a hydrophobic chain of a surfactant is repelled and assembled to a pore wall core. When the bilirubin adsorption material adsorbs albumin-bilirubin, the adsorption effect is better than that of MFC / QCS frozen gel, and the adsorption capacity and the adsorption rate are enhanced along with the increase of the concentration of albumin.

The invention relates to the technical field of blood purification materials, in particular to a zirconium-titanium bimetal MOF hollow microsphere adsorbent and a preparation method and application thereof. The adsorbent is based on a Zr / Ti bimetallic node and a phosphonic acid-carboxylic acid mixed ligand, a composite MOF hollow microsphere structure with high stability and high specific surface area is constructed through a solvothermal and polymerization synergistic reaction in a water-in-oil suspension system, a phosphonic acid group and Zr are strongly coordinated to improve the stability of a framework, and the composite MOF hollow microsphere structure has a high specific surface area. The carboxylic acid group keeps the hydrophilicity of the pore channel; meanwhile, a cross-linked network of acrylamide and N, N '-methylene bisacrylamide penetrates through crystal gaps in the balling process, so that the material has excellent mechanical strength and fluid stability; and a sulphobetaine amphoteric polymer brush layer is constructed on the surface of the MOF through endogenous initiation point modification and surface in-situ controllable free radical polymerization technologies, so that protein adsorption and platelet adhesion are effectively inhibited, the blood compatibility is remarkably improved, and meanwhile, the channel accessibility is maintained to promote rapid diffusion and adsorption of bilirubin.

The invention discloses calculus bovis combining in-vitro cultivation and in-vivo cultivation and a preparation method. The preparation method specifically comprises the following steps: (1) preparing an escherichia coli mixed bacteria solution; (2) preparing composite mucoprotein paste; and (3) carrying out in-vivo cultivation of the calculus bovis. Bacteria are preserved in the composite mucoprotein paste, and the composite mucoprotein paste has viscosity and is used as condensation nuclei for inducing calculus bovis condensation. Compared with the traditional crystal nucleus, the scheme can better protect the activity of the strain, the effect of protecting and slowly releasing the strain is achieved by using an embedding method, and strain inactivation caused by direct contact of the strain with ox bile is avoided. The ox bile simulation liquid with the best escherichia coli amplification effect is used as a bacterial liquid amplification culture medium injected into the ox gall bladder; after the mixed solution is injected, the 10% calcium chloride solution is injected again, so that a sticky bezoar bed is formed; the bilirubin content of the prepared bezoar is greater than or equal to 30%, and the cholic acid content is greater than or Each cattle cattle yellow is greater than 8g of calculus bovis; the success rate of calculus bovis preparation is greater than 90%.

The utility model discloses a bilirubin adsorption column saturation detector, which relates to the technical field of bilirubin adsorption treatment and comprises a light-emitting device and a detection device, the light-emitting device is provided with a first light-emitting end and a second light-emitting end, and the detection device is provided with a first detection end and a second detection end. The first light-emitting end is constructed to enable light to penetrate through a plasma input tube of the bilirubin adsorption column and irradiate the first detection end, the first detection end is used for detecting the illumination intensity of the light penetrating through the plasma input tube, and the second light-emitting end is constructed to enable light to penetrate through a plasma output tube of the bilirubin adsorption column and irradiate the second detection end; the second detection end is used for detecting the illumination intensity of the light passing through the plasma output tube. The detector can more accurately detect the saturation of the adsorption column in real time in the adsorption treatment process of the adsorption column.

The invention relates to the technical field of biomedical detection, and discloses a portable urine component detection system and method based on a fluorescence sensor array. The system comprises a fluorescence sensor array, different fluorescence probes are used for detecting different trace components in urine, and fluorescence color gradient changes are generated through a probe-target object reaction, so that bilirubin, glucose and albumin in the urine are detected. The detection system comprises a micro-fluidic chip which integrates a capillary driving channel and a colorimetric reaction area and is used for sample transmission and reaction area positioning; an ultraviolet excitation light source and a multi-angle optical sensor are arranged in the camera obscura type color acquisition module, and the camera obscura type color acquisition module eliminates ambient light interference and captures fluorescence chromaticity signals; and the intelligent data processing system is integrated with an intelligent analysis end of the smart phone, establishes a quantitative relationship between chromaticity parameters (RGB and hue saturation) and the concentration of a target object through a machine learning model, and outputs a detection result in combination with an ambient light correction algorithm.

An eyeshade structure for bilirubin detection includes a body, a first detection module, and a second detection module. The body includes a bottom plate and a side wall. The side wall is connected to the bottom plate and forms an accommodating space with the bottom plate. The first detection module is arranged on the bottom plate. The second detection module is arranged on the bottom plate and is adjacent to the first detection module. The first detection module and the second detection module each include a circuit board, a light-emitting element, an inner photographing element, and an outer photographing element. The circuit board is connected to the bottom plate. The light-emitting element is electrically connected to the circuit board. The inner photographing element is electrically connected to the circuit board. The outer photographing element is electrically connected to the circuit board.

The invention relates to the technical field of medical monitoring equipment, in particular to an intelligent drainage monitoring system and method after a hepatotomy based on multi-modal sensing, and the system comprises a sensing data collection module, a signal processing module, a data fusion analysis module and an abnormity recognition early warning module. The sensing data acquisition module comprehensively acquires pressure, component and diffusion state data of drainage liquid by utilizing an optical fiber pressure sensor array, a micro spectral analysis device and a biological impedance sensor, the signal processing module converts sensing data into digital signals, and the data fusion analysis module performs fusion analysis on the drainage liquid based on the signals. According to the system, the content of bilirubin and hemoglobin in drainage liquid and the diffusion state of the bilirubin and hemoglobin in the abdominal cavity are recognized and comprehensively analyzed, the abnormity recognition and early warning module compares an analysis result with a preset normal index, the abnormal condition is determined, an early warning signal is generated, the intelligence and accuracy of drainage management are effectively improved, and the working efficiency is improved. Postoperative complications can be reduced, and the rehabilitation quality of a patient can be improved.

The application relates to the technical field of biological pharmacy, and discloses a method for preparing high-bionic bovine bezoar by using a 3D printing technology and application. The preparation raw material of the high-bionic bovine bezoar comprises the following components: fermented bovine bile, saturated calcium hydroxide, hydroxypropyl methyl cellulose, bilirubin, cholic acid, deoxycholic acid and taurine; wherein the fermented bovine bile is obtained by fermenting bovine bile by using escherichia coli. The high-bionic bovine bezoar prepared by using the preparation raw material to perform 3D printing can be comparable to natural bovine bezoar in terms of components, effects and appearance, the main pharmaceutical components can be adjusted according to actual requirements, the production cost of the bovine bezoar can be greatly reduced, and the demand of people for the bovine bezoar can be met.

The invention relates to the technical field of blood purification, and discloses a blood adsorption device for removing bilirubin, comprising an upper end hemodialyzer and a lower end hemodialyzer connected to the upper end hemodialyzer through a connecting sleeve, wherein the liquid outlet of the upper end hemodialyzer is connected to a bilirubin adsorber through an adsorption circulation tube; by integrating the upper end hemodialyzer, the lower end hemodialyzer and the bilirubin adsorber together, while removing bilirubin, the blood is dialyzed and purified, thereby further reducing the burden on the kidneys; and only one puncture is required to simultaneously remove bilirubin and dialyze the blood, thereby reducing the pain of the patient; in addition, a mechanism for monitoring and controlling the reflux thereof is added during the adsorption and dialysis process, so that the concentration of bilirubin and other substances in the blood can be automatically monitored, and the blood can automatically reflux or pass according to the monitoring data, thereby greatly improving the intelligence and adsorption effect of the device.

The invention discloses a compound applied to a hemolytic agent and a composition thereof, which are characterized in that a compound which has weak reducibility and can be combined with hemachrome oxygenase to limit enzymatic activity is introduced into a formula of the hemolytic agent, so that the combination ratio of saponin and hemachrome oxygenase is reduced; according to the present invention, the dependence degree of the heme-biliverdin-bilirubin oxidation reduction system on the saponin is limited, the compatibility of the hemolytic agent formula on different batches of commercial saponin products is improved, and the weak reducibility of the compound avoids the oxidation problem of the saponin on the premise of no influence on the disulfide bond of the antibody, such that the heme-biliverdin-bilirubin oxidation reduction system can be easily obtained. Finally, the effects of reducing batch-to-batch clinical deviation of the hemolytic agent and improving the long-term stability of the hemolytic agent are achieved.

A point-of-care testing (POCT) system comprising an analyzer, a measurement cartridge, and a calibration cartridge for calibrating the measurement cartridge is described. The measurement cartridge comprises at least one electrochemical sensor for measuring the one or more properties of the blood sample, and the calibration cartridge comprises a similar at least one electrochemical sensor and at least one sealed blister containing calibration fluid for calibrating the measurement cartridge. Examples of properties of the blood sample may be pH, blood gases, electrolytes, and metabolites like glucose and creatinine. The measurement cartridge may also comprise an optical chamber for measuring for example, bilirubin and hemoglobin species, for which the analyzer comprises stored calibration algorithms. The optical chamber may be disposed in any location in the measurement cartridge, for receiving a portion of the blood sample.

The present invention relates to a pharmaceutical composition for preventing or treating inflammatory diseases comprising a compound of chemical formula 1, and to a cosmetic composition comprising the compound. By comprising the compound of chemical formula 1, the present invention can protect cells and reduce inflammation by removing reactive oxygen species and suppressing inflammatory cytokines in a non-toxic manner.

The invention discloses a protein / polysaccharide composite dialysis membrane based on in-situ loaded lanthanum carbonate and application of the protein / polysaccharide composite dialysis membrane, a lanthanum carbonate loaded protein / polysaccharide composite aggregate is used for modifying a porous filter membrane, and the dialysis membrane with a lanthanum carbonate loaded protein / polysaccharide self-assembled membrane as a compact skin layer and the porous filter membrane as a supporting layer is formed. By integrating a membrane separation mechanism of hemodialysis and an adsorption principle of blood perfusion, the skin layer of the dialysis membrane has molecular sieve separation and chemical adsorption functions, efficient removal of molecular uremic toxins (phosphorus, creatine liver, bilirubin, p-cresol sulfate and indol sulfate) with different sizes is synergistically realized, macromolecular proteins in blood are effectively intercepted by pore size screening, and the dialysis membrane has the advantages of simple structure, low cost and high efficiency. According to the present invention, by using the adsorption effect of the functional group on the surface of the membrane, the high removal rate on the protein-bound urotoxin is achieved, the high removal effect on phosphorus is particularly achieved, the blood compatibility is considered, the large-scale preparation is easily achieved, and the new idea is provided for the development of the high-performance blood purification material.

The invention discloses a human bilirubin content detection method and device, a storage medium and a server. The human bilirubin content detection method comprises the following steps: acquiring face video data; face blood filling degree caused by blood pulsation in face video data is identified to determine spectrums with different wavelengths, and a target dynamic spectrum is extracted from the spectrums; performing model training based on the target dynamic spectrum by using a partial least squares regression algorithm to obtain a bilirubin detection model; receiving a to-be-detected face video configured by a to-be-detected user, inputting the to-be-detected face video into the bilirubin detection model, and predicting to obtain the human bilirubin content of the to-be-detected user; the technical problem that the intelligent level is not high due to the fact that the corresponding spectrum cannot be extracted through analysis of the face video is solved.

The invention belongs to the field of medicine, and discloses application of bilirubin in preparation of medicine for treating ischemic cerebrovascular diseases, and the bilirubin can improve cognitive impairment caused by the ischemic cerebrovascular diseases; the severity of demyelination caused by ischemic cerebrovascular diseases is improved, the area of cerebral infarction is reduced, and the severity of neurological impairment caused by ischemic cerebrovascular diseases is relieved; inflammation, caused by ischemic cerebrovascular diseases, in blood and brain tissues is relieved, and activation of B cells and mediated immune response are inhibited; the oxidative stress of B cells is improved; the microglial cell activation caused by ischemic cerebrovascular diseases is relieved; the neuroinflammation reaction caused by ischemic cerebrovascular diseases is relieved. Good clinical application values are realized.

This invention provides a process for extracting biliverdin hydrochloride through bio-fermentation and a method for synthesizing bilirubin. By studying the complex system of biliverdin obtained through microbial bacterial fermentation, a process for separating and purifying biliverdin was established, and the obtained biliverdin was further used to synthesize bilirubin. This process is simple to operate, uses common resins, employs solvents with low toxicity that are easily recovered and reused, utilizes low-energy membrane separation equipment for concentration, and uses conventional reaction reagents as reducing agents. The process has low operating costs, is environmentally friendly, and yields high-purity and stable-yield biliverdin and bilirubin products, demonstrating excellent prospects for industrial application.

The invention relates to the technical field of bilirubin extraction, in particular to a bilirubin extraction-based heating and cooling tank for biological pharmacy, which comprises a tank body, a stirring mechanism is arranged in the tank body, one end of the top of the tank body is communicated with an exhaust pipe, and a plurality of uniformly distributed exhaust holes are formed in the top end of the exhaust pipe. A shielding seat is fixedly mounted at the top of the exhaust pipe, the exhaust pipe is fixedly sleeved with a collecting box, a defoaming mechanism and a pushing mechanism are arranged in the exhaust pipe, and a defoaming agent spraying mechanism is arranged in the collecting box. The device has the beneficial effects that the defoaming mechanism is arranged in the exhaust pipe, when the foam amount exceeds the processing capacity of a defoaming paddle and is sprayed out from the exhaust pipe, the two defoaming plates in the defoaming mechanism pierce and eliminate the foam, and in the process of extracting bilirubin through pig bile in biological pharmacy, the defoaming effect is good. And the problem of bilirubin loss caused by the fact that foam sprayed out of the exhaust pipe carries part of bilirubin is avoided.

Disclosed herein are improved pluripotent stem cell-derived multi-zonal liver organoids, having two or more hepatocyte subpopulations, and methods of producing the same, by coculturing a first human liver organoid (HLO) with a bilirubin-treated second HLO, and contacting the co-cultured HLOs with bilirubin for a period of time sufficient for the co-cultured HLOs to self-assemble and fuse into interconnected dual organoids. The first HLO can be an ascorbateenriched HLO, such as a functional L-gulonolactone oxidase (GULO)-expressing HLO. In particular embodiments, the hepatocyte subpopulations can include zone 1 and zone 3 hepatocyte subpopulations, or zone 1, zone 2, and zone 3 hepatocyte subpopulations. Also disclosed are methods of studying or treating a liver-related disease or disorder using the described multi-zonal liver organoids.

The utility model discloses a chloroform recovery device for bilirubin production, which comprises a heating tank, the upper end of the heating tank is provided with a cover, the upper end of the cover is fixedly connected with a feed pipe, and the cover is provided with a recovery component for recovering chloroform; according to the utility model, the thermometer is arranged and is used for detecting the temperature in the heating tank and transmitting the temperature to the controller, and the controller is used for controlling the start and stop of the electric heating plate, so that the temperature in the heating tank is higher than the boiling point of chloroform and lower than the boiling point of water; chloroform in the device is changed into steam, the chloroform steam is injected into a condenser through a second air outlet pipe, a connecting ring and an air inlet pipe, the condenser performs condensation, the condenser injects chloroform liquid into a storage block through a liquid outlet pipe, the storage block recovers chloroform, and the chloroform is convenient to reuse.