32 results about "Creatininase" patented technology

The present invention generally relates to the treatment of uremic toxins in vivo using uremic toxin-treating enzymes, and / or cells capable of producing uremic toxin-treating enzymes or otherwise reacting with uremic toxins. Non-limiting examples of cases where the treatment of uremic toxins is desired include renal disease or dysfunction, gout, subjects receiving chemotherapy, or the like. In one aspect, the treatment includes an oral delivery composition able to reduce the blood concentration of one or more non-protein nitrogen compounds in vivo. The composition, in some cases, may comprise one, two, or more uremic toxin-treating enzymes, such as urease, uricase or creatininase. The oral delivery composition may be able to deliver the uremic toxin-treating enzymes, substantially undigested, to the intestines, where the enzymes can interact with uremic toxins transported to the intestines from the bloodstream. In another aspect, the treatment includes an oral delivery composition comprising a cell able to reduce the concentration of one or more uremic toxins in vivo. In some cases, the cell may be designed to overexpress one, two, or more uremic toxin-treating enzymes, such as urease, uricase or creatininase, for example, by transfecting the cell with a corresponding gene. In some embodiments, a species able to react with or otherwise sequester by-products of the uremic toxin-treating enzyme reactions may be included with the oral delivery composition. For example, if the by-product is ammonium, the species may be a sorbent able to adsorb ammonium, an enzyme able to react with the ammonium, or the like.

The invention relates to a kit and a method for assaying creatinine. The kit includes a first reagent group and a second reagent group. The first reagent group includes creatinase, sarcosine oxidase, chromogen and catalase; the second reagent group includes creatininase, 4-amino antipyrine and peroxidase. The chromogen is selected from N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline or a sodium salt thereof, and N-ethyl-N-(2-hydroxyl-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and the method can reduce interference during the detection of the creatinine due to the calcium dobesilate and / or etamsylate that exist in a sample.

The invention relates to a composition for eliminating the interference of calcium dobesilate medicine on creatinine enzymatic detection, comprising: R1 reagent, R2 reagent and polymer oxidant; the polymer oxidant is polymer peroxyacid, polymer One or more of selenium oxides, polymer chlorosulfides, N-imides, water-soluble tetrazole, and Dess Martin oxidants. The polymer oxidant in the composition disclosed in the present invention can interact with the calcium dobesilate in the sample to be tested, so that the calcium dobesilate loses the effect of interfering with the detection of creatinine by enzymatic method, and improves the detection by sarcosine oxidase method Accuracy, thus solving the problem of serious negative interference of high concentration of calcium dobesilate in the patient's blood to the test results of enzymatic creatinine during the period when the patient is taking calcium dobesilate.

The invention relates to a use method for a creatinine content detection kit, which includes the following steps: adding creatinine of different concentrations to a buffer solution with the pH value of 7.3 and containing 100 mM of MHEPES, 150 mM of sodium chloride, 5 mM of potassium chloride and 1.3 mM of calcium chloride; obtaining and metering 150 muL sample solution through a capillary tube, inserting the tube in the kit and measuring through a capillary tube. The method provided by the invention has high selectivity; creatininase as proteinase has high specificity to a reactant, only catalyzes the hydrolysis reaction of creatinine, and has no catalytic activity to other oligomers; as three high selectivity enzymes are related during the detection, the selectivity in the method is increased exponentially, the final response of the kit is fluorescent response, and compared with other methods for detecting color change, the method adopting the fluorescent response has the advantage that the inherent characteristic of fluorescent response endows the method with higher sensitivity and anti-interference capability.

The invention discloses a creatinine detection reagent which is characterized by comprising a diluent and a reaction reagent, wherein the diluent is composed of buffer, surfactant, preservative, anti-bilirubin interference agent and vitamin C oxidase; and the reaction reagent is composed of buffer, creatinase, sarcosine oxidase, peroxidase, N-ethyl-N-(3-propylsulfo)-3-methylaniline, creatininase, 4-aminoantipyrine, preservative and freeze-drying protective agent. The detection reagent disclosed by the invention has favorable sensitivity, accuracy, precision and linearity, and can completely satisfy the clinical examination requirements.

The present invention relates to a creatinine determination kit and a creatinine determination method. The kit comprises a reagent group 1 and a reagent group 2, wherein the reagent group 1 comprises creatinase, sarcosine oxidase, chromogen and catalase, and the reagent group 2 comprises creatininase, 4-aminoantipyrine and peroxidase, wherein the chromogen is N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline or a sodium salt thereof. According to the present invention, with the kit and the method, the interference caused by calcium dobesilate and / or etamsylate existing in the sample can be reduced during the creatinine determination.