Creatinine content determination method and creatinine diagnosis kit
A technology for diagnostic kits and assay methods, which is applied in the field of medical examination and assay, and can solve the problems of reduced test accuracy, inconvenient test, slow time, etc.
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Embodiment 1
[0078] Embodiment one (single agent)
[0079] The creatinine diagnostic kit of the present embodiment comprises:
[0080] Buffer 80mmol / l
[0081] Adenosine triphosphate 2mmol / l
[0082] Phosphoenolpyruvate 1mmol / l
[0083] Reduced coenzyme 0.2mmol / l
[0084] Creatinase 6000U / l
[0085] Creatine kinase 6000U / l
[0086] Pyruvate kinase 8000U / l
[0087] Lactate dehydrogenase 20000U / l
[0088] Stabilizer 50% (total volume)
[0089] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is negative reaction.
[0090] After the sample and reagents are added and allowed to mix, the following reactions occur:
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[0093]
[0094]
[0095]
[0096] The final reactant is placed under a biochemical analysis instrument, and the decrease in absorbance at ...
Embodiment 2
[0098] Embodiment two (two doses)
[0099] The creatinine diagnostic reagent of the present embodiment has:
[0100] Reagent I
[0101] Buffer 100mmol / l
[0102] Adenosine triphosphate 4mmol / l
[0103] Phosphoenolpyruvate 2mmol / l
[0104] Reduced coenzyme 0.25mmol / l
[0105] Creatine kinase 8000U / l
[0106] Pyruvate kinase 12000U / l
[0107] Lactate dehydrogenase 25000U / l
[0108] Stabilizer 50% (total volume)
[0109] Reagent II
[0110] Buffer 100mmol / l
[0111] Creatinase 8000U / l
[0112] Stabilizer 50% (total volume)
[0113] When measuring creatinine content, the temperature is controlled at 30°C, the reaction time is 15 minutes, the main wavelength of the test is 340nm, the secondary wavelength of the test is above 405nm, the volume ratio of the creatinine sample to the reagent is 1 / 25, and the reaction direction is negative reaction.
[0114] The specific measurement steps are:
[0115]
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[0119]
[0120]The final rea...
Embodiment 3
[0122] Embodiment three (three doses)
[0123] The creatinine diagnostic reagent of the present embodiment is three doses, has:
[0124] Reagent I
[0125] Buffer 120mmol / l
[0126] Adenosine triphosphate 6mmol / l
[0127] Phosphoenolpyruvate 3mmol / l
[0128] Reduced coenzyme 0.3mmol / l
[0129] Stabilizer 50% (total volume)
[0130] Reagent II
[0131] Buffer 120mmol / l
[0132] Creatine kinase 10000U / l
[0133] Pyruvate kinase 16000U / l
[0134] Lactate dehydrogenase 30000U / l
[0135] Stabilizer 50% (total volume)
[0136] Reagent III
[0137] Buffer 120mmol / l
[0138] Creatinase 10000U / l
[0139] Stabilizer 50% (total volume)
[0140] Set on the automatic biochemical analyzer: temperature 25°C, reaction time 20 minutes, test main wavelength 340nm, test sub-wavelength above 405nm, the volume ratio of the creatinine sample to the reagent to be tested is 1 / 25, and the reaction direction is negative reaction.
[0141] The specific measurement steps are:
[0142] ...
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