122 results about "Negative reaction" patented technology

The method of reducing loaded metal catalyst with low temperature plasma includes the steps of: 1) dissolving active metal salt component in deionized water or distilled water to form solution with metal component content of 0.01-20 wt%, filling solution into catalyst carrier, letting stand at room temperature for 8-24 hr and drying at 40-110 deg.c for 2-10 hr; and 2) setting the catalyst between two electrodes of plasma apparatus, introducing inert gas, air or oxygen as plasma discharge gas into discharge tube of pressure of 50-10000 Pa, and applying DC or AC voltage of 100-20000 V across the electrodes to process for 5-200 min. The low temperature plasma contains great amount of electrons, has powerful reducing capacity to reduce metal ion into metal simple substance and has no negative reaction appearing at high temperature. The process is simple, short in reduction time, without need of chemical reductant and environment friendly.

A chargeable alkali metal-sulfur liquid flow battery comprises a positive electrode chamber portion, a diaphragm and a negative electrode chamber portion, wherein the positive electrode chamber portion comprises a positive electrode reaction chamber and a liquid storage tank communicated with a positive electrode chamber pipeline. The positive reaction chamber comprises a positive electrode collector and positive electrode sizing, and the positive electrode sizing used for a positive electrode circulates between the positive electrode reaction chamber and the liquid storage tank. The negative electrode chamber portion comprises a negative electrode reaction chamber comprising a negative electrode, a negative electrode collector and negative electrode electrolyte, and the diaphragm is a single-ion conductor diaphragm and is arranged between the positive electrode reaction chamber and the negative reaction chamber. Only one working ion rather than any other substances such as non-working ions is guaranteed to conduct between the positive electrode and the negative electrode. The positive electrode sizing is composed of positive electrode electrolyte and positive active matters mixed in the positive electrode electrolyte, and the positive active matters are one or more kinds of MxSy (M=Li or Na; 0(x< / =2; 0(y< / =12).

The present invention provides one kind of gene, recombinant plasmid and polypeptide of EB virus resisting tumor polypeptide. The coding inducing polypeptide gene and coliform group gene are connected via operation to obtain polynucleotide sequence expressing recombinant antitumor polypeptide. The nucleotide sequence of the coding inducing polypeptide is inserted into coliform group gene by means of double strand oligomeric nucleotide point mutation technology to form the said recombinant plasmid. The obtained recombinant plasmid is transfected into engineering colibacillus TG1 to obtain engineering bacillus cell capable of generating recombinant antitumor polypeptide; and through mass proliferation, centrifugal precipitation and other steps, recombinant antitumor polypeptide is obtained. The antitumor polypeptide has specific targeting property, high efficiency of killing solid tumor, no attack to health cell, low toxicity and less negative reaction.

Microarray devices fabricated using microfluidic reagent distribution techniques are provided. As described herein, the invention encompasses microfluidic microarray assemblies (MMA) and subassemblies and methods for their manufacture and use. In one embodiment first and second channel plates are provided which may be sealed to a test chip in consecutive steps. Each channel plate includes microfluidic channels configured in a predetermined reagent distribution pattern. For example, the first channel plate may have a radial (linear) reagent distribution pattern and the second channel plate may have a spiral (curved) reagent distribution pattern, or vice versa. In one embodiment the first channel plate is connected to the test chip and at least one first reagent is distributed on the test chip in a first predetermined reagent pattern. The first reagent is then immobilized on the test chip. Next, the first channel plate is removed, the second channel plate is connected to the test chip and at least one second reagent is distributed on the test chip in a second predetermined reagent pattern. The first and second reagent patterns intersect to define a plurality of microarray test positions on the test chip. In one embodiment, the first reagent may comprise a plurality of separate probes each distributed to selected test position(s) of the microarray and the second reagent may comprise a plurality of test samples each distributed to selected test position(s) of the microarray. Positive or negative reactions between the probes (or other first reagent) and test samples (or other second reagent) may then be detected at the microarray test positions. For example, hybridization between selected nucleic acid probes and selected nucleic acid samples may be detected at particular test positions. The invention thus provides an efficient means to fabricate high density multi-probe, multi-sample microarrays. Preferably the test chips and channel plates are circular and centrifugal force is used to achieve fluid flow through the microfluidic channels, such as by rotating the MMA in a disc spinner.

The invention provides a lithium double-fluid flow battery, which comprises a porous positive current collector, a positive reaction cavity, a diaphragm, a metal lithium negative plate, a negative reaction cavity, a positive suspension and a negative electrolyte, wherein a gap with a certain distance exists between the diaphragm of the battery and negative metal lithium; the gap is fully filled with the negative electrolyte which continuously or intermittently flows; and a solid-liquid composite dual-diaphragm structure is formed by the diaphragm and the negative electrolyte. Through continuous or intermittent washing by the electrolyte, the problem of potential safety hazards caused by lithium dendrites generated by a metal lithium negative electrode in charge-discharge processes is solved; and meanwhile, the lithium double-fluid flow battery plays roles in purifying the negative electrolyte, cleaning the diaphragm and adjusting the concentration of the positive suspension.

The present invention relates to one kind of broad-spectrum cancer treating Chinese medicine capsule and its preparation process. It is prepared with trichosanthes root, edible tulip bulb, zedoary, pinellia tuber, grisaema tuber and other Chinese medicinal materials. It has the functions of nourishing Yin, promoting blood circulation, warming Yang, promoting urination, dredging meridian and dissipating mass. The animal experiment and the clinical application show that the Chinese medicine has obvious curative effect, no negative reaction and no toxic side effect.

The invention discloses a high-voltage battery containing an aqueous electrolyte and a preparation method of the high-voltage battery. The battery uses an appropriate diaphragm to lead positive reaction and negative reaction to generate in different environments, so that the positive reaction has low reduction potential, the negative reaction has high oxidation potential, and the battery has high working voltage. The battery mainly comprises a positive electrode, a negative electrode, a positive electrolyte, a negative electrolyte and the diaphragm. By reasonably selecting the positive electrode, the negative electrode, the diaphragm and the electrolytes corresponding to the positive electrode and the negative electrode, the battery having the working voltage higher than 2.0V can be obtained.

The invention discloses an oil-tea camellia husk polysaccharide which has the following characteristics: a. physicochemical properties: the polysaccharide is brown powder with small odor and is easily-soluble in water and not soluble in organic solvents such as chloroform and ether, the polysaccharide is orange if reacting with phenol-sulfuric acid, shows a negative reaction with Fehling reagent and shows a negative reaction with iodine-potassium iodide; b. composition of the polysaccharide: the content of the polysaccharide is 30 percent to 50 percent; the distribution range of molecular weight is 7,000 to 200,000,0Da; the polysaccharide is prepared by the following six kinds of monosaccharide: glucose, fucose, arabinose, galactose, seminose and rhamnose. The oil-tea camellia husk polysaccharide is used as free radical scavenger or used for preparing medicaments with anti-tumor function for curing tumors.

The present invention relates to a kind of Chinese medicine with functions of nourishing vital energy, warming Yang and invigorating spleen and its preparation process. The medicine is prepared with ten kinds of Chinese medicinal materials, including ginseng, prepared rhizome of rehmannia, white atractylodes rhizome, astragalus root, tuckahoe, white peony root, angelica, etc. The Chinese medicine with functions of nourishing vital energy, warming Yang and invigorating spleen is used in treating vital energy and blood deficiency caused by radiotherapy and chemotherapy of tumor patient, can reduce the toxic side effect of radiotherapy and chemotherapy and raise immunity, and has no obvious negative reaction.

The invention discloses a CO2 packaged low-temperature low-dose irradiation refreshing method for edible mushrooms, comprising the following steps of: step I, deairing an edible mushroom film and charging CO2 for packaging; step II, precooling the edible mushroom packaged in the step I to be 0-4 DEG C; and step III, adopting 60Co r rays or 5-10MeV electron beam rays to perform irradiation to the edible mushroom in the step II, wherein the irradiation absorbed dose is 0.1-1.0kGy, and the inequality of irradiation dose is less than 1.25. In the invention, the comprehensive irradiation refreshing method maintaining the original color, fragrance, taste and state of the edible mushrooms is adopted, when the irradiated edible mushrooms are stored at normal temperature (10-25 DEG C), the original color, fragrance, taste and state are maintained for 4 days, and when the irradiated edible mushrooms are stored at 0-4 DEG C, the original color, fragrance, taste and state are maintained for 14 days. The packaging filled with CO2 is adopted, CO2 can effectively inhibit breathing of the edible mushrooms, irradiation is performed when the edible mushrooms are precooled to be 0-4 DEG C, at 0-4 DEG C, the breathing of the edible mushrooms can be reduced, growth reproduction of harmful microorganisms polluting the edible mushrooms is inhibited, and negative reaction of edible mushrooms, caused by irradiation, at the temperature can be reduced.

The present invention relates to one kind of infantile lung-invigorating asthma-relieving ointment and its preparation process. The present invention features the materials for the ointment including ginseng, roasted astragalus root, tuckahoe, white atractylodes rhizome, ledebouriella root and other ten kinds of Chinese medicinal materials in certain weight proportion. The medicine of the present invention has good patient compliance, good taste, low cost, high curative effect, no negative reaction and toxic side effect, convenient taking and less recurrence.

The invention provides an organic matter detection device and method for an anion proton counter-transference reaction mass spectrometry. The organic matter detection device comprises a gas discharge anion source, a reaction tube, an anion mass spectrometry detection system, a discharge power source, an ion extraction power source and an ion drift power source, wherein the reaction tube is positioned between the gas discharge anion source and the anion mass spectrometry detection system; the gas discharge anion source consists of a discharge cathode, a discharge anode, a discharge gasket and an extraction gasket, the discharge cathode is positioned at the top end of the gas discharge anion source, the discharge gasket is positioned between the discharge cathode and the discharge anode, and the extraction gasket is positioned between the discharge anode and the reaction tube; a discharge gas inlet is formed in the discharge cathode, and a discharge gas pumping hole is formed in the extraction gasket; the reaction tube consists of a sample introduction electrode, a tail electrode, a plurality of ring electrodes and ring gaskets, the negative pole of the discharge power source is connected in series with a resistor and connected with the discharge cathode, and the positive pole of the discharge power source is connected with the discharge anode. Through electric field extraction and guiding of negative reaction ions, such as OH<-1>, the organic matter detection device and method for the anion proton counter-transference reaction mass spectrometry are successfully realized.

The invention relates to a method for evaluating water quality health risk with a chromium ion as a standard toxic substance, comprising the following steps: a toxicity test is conducted on biological fish, if dead fish occurs in 96h, Cr<6+> concentration is used for representing water quality toxicity, and if dead fish does not occur, a luminescent bacteria toxicity test is conducted; if the luminescent inhibition rate is higher than 40%, the Cr<6+> concentration is used for representing the water quality toxicity, and if the luminescent inhibition rate is lower than 40%, an SOS / umu test is conducted; if a test result is positive reaction, the Cr<6+> concentration is used for representing the water quality toxicity, and if a test result is negative reaction, a broad bean root tip micronucleus test is conducted; and if a micronucleus permillage ratio is more than 1.5, the Cr<6+> concentration is used for representing the water quality toxicity, and if the micronucleus permillage ratio is less than 1.5, a unicellular gel electrophoresis test is conducted and the Cr<6+> concentration is used for representing the water quality toxicity. In the invention, the steps are closely related to each other so that both the low pollutant content and the high pollutant content in water quality can be detected, the detection time is shorter, the result is accurate and easy to unify, and quantification evaluation of the water quality toxicity can be realized.

The invention relates to a carcass and carcass product innocent treatment method. A fermented body composed of bacillus natto, saccharomycetes and bacillus subtilis is added into a compost material, so that the compost material has strong fermentative capability and ability of inhibiting pathogenic microorganism growth; the built compost system can quickly ferment and quickly degrade animal tissues, greatly ascends the compost temperature within a short period of time and kills pathogenic bacteria and viruses. Meanwhile, the ability of the fermented body for inhibiting the pathogenic microorganism growth also further facilitates killing of the pathogenic microorganisms, so that the targets of improving the degradation efficiency and the compost treatment safety are achieved. Through test, the treatment period of the method disclosed by the invention does not exceed 60 days at most; the compost temperature can be up to over 60 DEG C at the third day of static composting, and can be up to 72 DEG C; the pathogenic microorganisms such as escherichia coli and salmonella enteritidis are not detected after a compost sampling test; the detection result of the viruses such as swine fevers, blue-eared diseases and 2-type porcine circovirus is negative reaction.

The invention discloses an airbag type pressure protection device for eliminating excessive water hammer pressure in long-distance water supply projects. The exterior of the device is composed of a metal airtight shell, and the interior is composed of an air bag type liner. The metal airtight shell is equipped with an air pressure detection instrument, and a connecting flange and a small section of steel pipe are installed at both ends. The device can be directly installed in the pipeline, and the front and rear can be set. Butterfly valves are used for inspection and maintenance. The device of the present invention is low in cost and easy to install and maintain. On the premise of meeting other adjustment and maintenance requirements, the compression and expansion characteristics of the airbag are used to form a large negative reflection boundary in the pipeline to reflect water hammer waves and change The propagation characteristics of the water hammer wave greatly relieve the water hammer pressure when the valve is adjusted to ensure the stable and safe operation of the water supply system, especially for long-distance gravity flow water supply projects, the effect is more significant.

The invention relates to a method for accurately and rapidly detecting antigens and antibodies in human serum in a trace mode. The method comprises the steps of preparing trace (1ml) known anti-erythrocyte antigen and antibody on a trace detection plate by utilizing an immunohematology principle, adding trace (1ml) unknown erythrocytes to be detected, and detecting the antigens on the surface of the erythrocyte membrane; or adding trace (1ml) unknown serum to be detected into the erythrocytes with the known trace (1ml) erythrocyte antigen, and detecting the antigens and antibodies on the surface of the erythrocyte membrane in the human blood. The reaction identifier is collected by the erythrocytes, the reaction with the collected erythrocytes is a positive reaction, the reaction without the collected erythrocytes is a negative reaction, the method is combined with a centrifugal machine, a spectrophotometer, a microscope and computer programs, and the erythrocyte antigens and the antibodies in the serum can be rapidly, accurately and automatically detected. According to the trace detection plate kit, the safety, high efficiency and accuracy of the clinical erythrocyte zygosity can be greatly improved, the detection cost is reduced, and the development of clinical hematology application is promoted.

The invention belongs to the field of batteries, and discloses a flow battery. The flow battery comprises a positive current collector, a negative current collector, a diaphragm, a positive reaction zone, a negative reaction zone, a positive reaction solution flowing through the positive reaction zone, a negative reaction solution reacting in the negative reaction zone, a positive solution storage tank, a first power supply promoting the positive reaction solution to flow through the positive reaction zone, and a second power supply promoting the negative reaction solution to flow through the negative reaction zone; the positive reaction solution comprises a positive electrolyte, and a positive active material and a positive conductive agent suspending in the positive electrolyte; the positive active material comprises a Zn<2+>-deintercalated functional material; the negative reaction solution comprises a negative electrolyte; and the work ion of each of the positive electrolyte and the negative electrolyte is Zn<2+>. The flow battery has the advantages of high energy density and low mechanical energy consumption rate; and the electrolytes have the advantages of non-toxicity, small corrosivity, abundant raw material reserves, low price and battery cost saving.

A fowl-kidney-form infectious bronchitis HN99 virus has excellent immunogenicity. The diameter of coronal virus particle is 90-105 nm, it has membrane and is radiant with 18 nm vomer protrusion, it has no interference for NDV La Sota virus multiplying and no agglutination for 1% fowl red blood cell; regression flow experiment leads to respiratory tract symptom and renal boss. Ill fowl virus isolate has positive reaction with HN99 positive blood serum, has negative reaction with NDV La Sota strain, IBDV B87 strain and AIV H9N2 strains; HN99 positive blood serum has positive reaction with T strains, Hubei strain, Tianjin strain and Shandong strain and has negative reaction with other viruses. It has exogenous pathogenic pollution.

The invention belongs to the related field of secondary battery preparation technology, and discloses a preparation method of a composite metal anode for a secondary battery. The preparation method comprises the following steps: forming a plurality of hoes in an array type through a photosensitive film on a thin film made of a current collector; depositing a metal electrode material internally corresponding to each hole; continuously filling a composite electrolyte solution on the surface of the metal electrode material, and enabling the surface of the metal electrode to be covered with the composite electrolyte in a solid state finally, wherein the side face of the composite electrolyte is fixed by the photosensitive film. The invention also discloses a corresponding product prepared through the technology. Through the preparation method and the product, the metal anode for the secondary battery with a protective film and a fixing structure at the same time can be prepared in convenient operation and high efficiency manners, and negative reaction and dendritic crystal production of the metal anode and the electrolyte can be inhibited at the same time, so that the harmful effects such as volume expansion and low energy density caused by the protective film can also be obviously reduced, and the composite metal anode is particularly suitable for application occasions of industrial large-scale production.

The invention relates to the gene mutation relevant to the paralysis agitans and the detecting method, comprising: extracting DNA sample; designing primer; expanding with PCR; analyzing nucleic acid by means of dHPLC technology; testing the gene order of the sample which is dHPLC positive reaction to definite the genetype; carrying out beta-actin comparison for the sample of repeated extanding negative reaction to eliminate extron deletion mutation. The parkin gene mutation is in the codon promoter of the parkin gene, the second basic group in the exon1, ATG mutated to ACG; on the intersection point of thefifth introne and sixth extron in the exon6, AG mutated to AC; in the sixth extron, the 727th basic group, G mutated to A; in the exon9, the 1016th basic group, C mutated to T; in the position of ring finger intermediate region, in the Znf C6HC zinc region, GCG mutated to CGTC. By applying the gene mutation and the detection method, the detection for the mutation site of the parkin fene is high-effective and low-cost, and the diagnose to the potential PD patient can be made in an early stage.

The present invention belongs to the field of medicine technology, and is especially one kind of heart failure treating Chinese medicine. The Chinese medicine is prepared with notoginseng, kudzu vine root, sealwort, ginseng, Chuanxiong rhizome and other 17 kinds of Chinese medicinal materials, and may be prepared into different pharmaceutically acceptable preparation forms, such as medicine powder, water bolus, honeyed bolus and capsule. The Chinese medicine, clinical application for years shows, has the functions of promoting blood circulation, invigorating vital energy, strengthening Yang and warming heart and kidney. It has high curative effect on angina pectoris, coronary heart disease, dilating heart disease and other heart troubles, no toxic side effect, no negative reaction.

The invention relates to a nucleotide sequence, a specific primer and a method for identifying physalis angulata. The nucleotide sequence for identifying physalis angulata disclosed by the invention is shown as SEQ ID NO.1. The specific primer for identifying physalis angulata has an upstream sequence ST3KZF shown as SEQ ID NO.2 and a downstream sequence ST3KZR shown as SEQ ID NO.3. The physalis angulata is identified by a conventional PCR method by utilizing the specific primer ST3KZF / ST3KZR. The sample amount is small, and the whole operation can be finished by virtue of a small amount of samples; the accuracy and sensitivity are high, the ST3KZF / ST3KZR refers to a specific amplification primer of the physalis angulata, and if the samples refer to other physalis plants, a negative reaction presents. The method is simple and convenient, the PCR technology is adopted for detecting, the time consumption is low, and the operation can be finished within 3-5 hours.

The invention discloses a longitudinal-rib-above-arranged steel-concrete-steel-shell-combining-beam cable-stayed bridge. The longitudinal-rib-above-arranged steel-concrete-steel-shell-combining-beam cable-stayed bridge comprises a main cable-stayed-bridge beam, the main cable-stayed-bridge beam is composed of a main span, side spans arranged on the two sides of the main span and transition sections arranged between the main span and the side spans; main towers are arranged on the two sides in the length direction of the main cable-stayed-bridge beam at intervals, wherein the main towers and the main cable-stayed-bridge beam are perpendicularly arranged; the tops of all the main towers are fixedly connected with the main cable-stayed-bridge beam through a plurality of stay cables, the main span is of a steel-concrete-combination whole steel box beam structure, and the side spans are of steel-concrete-combination-steel-shell-concrete-beam structures. According to the longitudinal-rib-above-arranged steel-concrete-steel-shell-combining-beam cable-stayed bridge, a longitudinal-rib-above-arranged steel-concrete-combination whole steel box beam is used for the main span, longitudinal-rib-above-arranged steel shell concrete beams are used in the side spans, bran-new steel concrete transition section modes are designed for the side spans, the large spanning capacity and the vertical rigidity are achieved, the construction speed is high, it is avoided that negative reaction force of the side spans appear, and the service life of the bridge face is prolonged.

The present invention discloses the application of Ixeris Sonchifolia Hance flavone compounds in preparing medicine for treating coronary heart disease, and the extraction process of Ixeris Sonchifolia Hance flavone compounds and five monomer compounds. Ixeris Sonchifolia Hance flavone compounds applied in medicine for treating coronary heart disease has the features of low toxic side effect, determined curative effect, safety and reliability. The medicine has no negative reactions of bleeding, allergy, causing hypertension, etc.

A device for the accelerated aging of wine consisting of a low direct-current voltage applied to inert electrodes is described herein. The device improves the taste of many wines by accelerating the natural aging process which is primarily a free-radical-induced reaction involving the polymerization and precipitation of polyphenols such as tannins, thus reducing the astringency and improving the taste of many full bodied wines. The device can be miniaturized for use with single servings or scaled up to be applied to large commercial batches. The device has the added feature of being able to reduce the sulfite content of some wines, reducing foul tastes caused by free sulfur dioxide and possibly reducing negative reactions to sulfite for some users of wine.

The device comprises an organic matter detection device and an organic matter detection method of a bipolar proton transfer reaction mass spectrometer. The organic matter detection device mainly comprises a common discharge ion source, two sets of positive and negative reaction tubes, positive and negative transition chambers and positive and negative mass spectrometers, and a set of common vacuum pump group. The organic matter detection method comprises the steps of: achieving the extraction of positive and negative reaction ions of H3O<+> and OH<-> by means of the discharge ion source, and realizing double efficient ionization of a matter to be detected; and accurately judging molecular weight of the matter to be detected according to a difference value (2) of charge-to-mass ratios of product ions ([MH]<+>, [M H] <->), and realizing accurate recognition and online highly-sensitive detection of the matter to be detected. Compared with a proton transfer reaction mass spectrometer and a proton counter-transfer reaction mass spectrometer, the set of the organic matter detection device is formed by the common discharge iron source, the vacuum pump group and the like, the comparability of obtained results is higher, and the cost is lower. The organic matter detection device and the organic matter detection method can realize the positive and negative ion cooperative recognition and highly-sensitive online detection of the organic matter of the bipolar proton transfer reaction mass spectrometer.

The invention relates to a nucleotide sequence and a molecular probe for identifying dendrobium officinale and applications of the molecular probe. The nucleotide sequence for identifying the dendrobium officinale is as shown in SEQ ID NO.1. An upstream nucleotide molecular probe SHF for identifying the dendrobium officinale is as shown in SEQ ID NO.2 and a downstream nucleotide molecular probe SHR is as shown in SEQ ID NO.3. The invention also provides the applications of the nucleotide molecular probe SHF / SHR in identification of the dendrobium officinale. In the applications of the nucleotide sequence and the molecular probe disclosed by the invention, few samples are used; the overall operation can be finished with use of few samples; the accuracy and the sensitivity are high; the SHF / SHR is a specific molecular probe for the dendrobium officinale, and a negative reaction is performed if the dendrobe is of another type; the method is simple; detection is carried out through a PCR (Polymerase Chain Reaction) technology and can be finished in a short time, particularly in half a day.

The invention relates to a novel steel truss beam composite structure. A steel-concrete combined bridge surface subsection is arranged at the side span or at the middle span of a main truss according to the requirements, and an all-steel beam structure subsection can be arranged at the middle span or at the side span of the main truss according to the requirements. In the invention, the all-steel truss beam subsection is adopted at the section with the deadweight required to be reduced, and the steel-concrete combined bridge surface subsection is adopted when the deadweight is required to be increased or the rigidness of the bridge beam is required to be increased. The characteristics of strong integrity and great rigidity of the steel truss beam-concrete combination are fully utilized in the invention, and problems of rigidity of the all-steel truss beam and negative reaction of the side span are solved. Therefore, the invention has the advantages that the stress rationality of double-purpose continual steel truss beams of wide-span highways and railways can be guaranteed, the material performance of steel and concrete is fully utilized, the cross section of each rod is reduced, and the requirements for the steel beams and thick sheets are reduced, so the steel material can be purchased easily. So the reduction of the whole project cost is realized.

The invention discloses a method for establishing a protein-protein interaction network by utilizing text data. The method is characterized by comprising the following steps: (1) establishing a protein set; (2) recording the probability values of interactions of every two proteins in the protein set; (3) establishing an initial network structure according to the probability values; (4) repeatedly selecting protein, giving feedback values of positive or negative reactions, iterating discontinuously on the initial network structure to obtain a final network structure. According to the method, the probability graph of a reaction network is established through reinforcement learning in the mode of repeatedly selecting and interacting on the basis of positive feedback, negative feedback and prohibited feedback; the probability graph is combined seamlessly with biological knowledge and biological data.

The invention provides a battery leakage detection method. The method includes following steps: step 1, injecting electrolyte and a tracer agent into a battery, and completing encapsulation of the battery; step 2, putting the battery obtained in the S1 into a closed container with a mass spectrometer, vacuumizing the container, holding pressure, filling gas, and repeating in this way; step 3, starting the mass spectrometer, detecting gas extracted from the closed container, and judging whether the battery leaks or not according to whether the tracer agent is present or not, wherein the traceragent is a substance containing at least one of elements 15N, 13C, 14C, 17O, 18O, 2H and 3H, does not corrode pole pieces, current collectors, pole lugs and an encapsulation material and does not react with the electrolyte or is not in negative reaction affecting performance of the electrolyte. By the method, defective batteries can be screened out conveniently and quickly to avoid potential safety hazards caused by the fact that the defective batteries having risk of electrolyte leakage go to clients.