44results about How to "Realize highly sensitive detection" patented technology

The invention discloses a preparation method and application of a framework nucleic acid synergistic enhanced click chemistry and DNAzyme catalytic electrode. According to the present invention, the azide group modified split G-quadruplex fragment A is assembled on the double-stranded DNA (dsDNA) edge of the DNA tetrahedron through the Hoogsteen hydrogen bond, such that the reasonable spatial distribution of the reactants is achieved, and the click chemical reaction efficiency on the interface is improved; under the catalytic condition of Cu (I), the alkyne group modified split G-quadruplex fragment B and the fragment A assembled on the tetrahedral scaffold form a complete G-quadruplex through a click chemical reaction. And further, the compound is compounded with Hemin, so that the formed G-quadruplex / hemin compound has peroxidase-like activity. Meanwhile, the nucleic acid framework with the strong electronegativity characteristic further enhances the response current of catalytic oxidation TMB, and sensitive detection of Cu < 2 + > and alkaline phosphatase is achieved.

The invention is suitable for the field of gas sensor detection, and provides a mixed potential type ammonia gas sensor and a preparation method thereof. The mixed potential type ammonia gas sensor comprises a layered nickel silicate sensitive electrode, a reference electrode and a solid electrolyte, the layered nickel silicate sensitive electrode and the reference electrode are positioned on the surface of the solid electrolyte; the layered nickel silicate sensitive electrode is prepared from three kinds of layered nickel silicate electrode slurry with different morphologies. According to the invention, the layered nickel silicate is used as the sensitive electrode, so that the response value to ammonia gas is obviously improved at room temperature; compared with an existing most advanced mixed potential type ammonia gas sensor, the high-temperature mixed potential type ammonia gas sensor has the advantage that the low-concentration response to ammonia gas can be remarkably improved at room temperature compared with other high-temperature mixed potential type ammonia gas sensors.

The application belongs to the field of optical fiber sensing, and particularly relates to a high-sensitivity optical fiber SPR traditional Chinese medicine active ingredient sensor and a manufacturing and using method thereof; the sensor comprises a core offset light injection area, a mode modulation and compression area and a coarse core light receiving area which are sequentially fused, the mode modulation and compression area is in three modes of a groove type of graded-index fiber, a taper spiral type of core offset fiber and a flat bottom partial sphere type of core offset fiber, gold films and target proteins are arranged on the mode modulation and compression area from inside to outside, an optical signal is injected into the mode compression and modulation area from the core offset light injection area, after modulation, the optical signal mode is concentrated and the light incidence angle is effectively modulated, the sensor sensitivity is greatly improved, transmission light contacts the gold films to generate an SPR effect, the target proteins are specifically combined with traditional Chinese medicine active ingredients, an SPR resonance valley moves, and specific detection of traditional Chinese medicine active ingredients is realized. The application solves the problems of low sensitivity and difficulty in specific detection of the current optical fiber SPR traditional Chinese medicine active ingredient sensor.

The application relates to a kind of cascade low-temperature single-photon detectors based on torsion semimetal materials, comprising: substrate, cascade structure and electrode;Wherein, the cascade structure is arranged on the substrate;The electrode is arranged on the cascade structure;The cascade structure is formed by the alternating stacking of dielectric layer and Dirac semimetal material;The outermost layer of the cascade structure from bottom to top and from top to bottom is the dielectric layer;The electrode includes a top gate electrode arranged at the top of the cascade structure and a plurality of source / drain metal contact electrodes arranged on the Dirac semimetal material.The beneficial effects of the application are: the Fermi level of the Dirac semimetal material used in the application is adjustable, the internal carrier concentration is controlled by the gate region, and the single-photon energy can excite to generate a photovoltage signal.

The application discloses a membrane sample injection ion funnel ionization source device and belongs to the technical field of mass spectrometry analyzers. The device comprises an ionization source cavity, the top of the ionization source cavity is provided with a vacuum ultraviolet lamp, and the bottom of the ionization source cavity is provided with a differential electrode; an ion repulsion electrode is arranged below the vacuum ultraviolet lamp, the ion repulsion electrode is arranged in parallel with the differential electrode, the ion repulsion electrode and the differential electrode are connected through insulating columns, the insulating columns are at least 3 and are perpendicular to the differential electrode, a direct current transmission electrode group is arranged on the inner side of the insulating columns, a tubular membrane is arranged on the outer side of the insulating columns, and the tubular membrane is arranged in position correspondence with the direct current transmission motor group; and an ion funnel device is arranged below the direct current transmission electrode group. The application proposes a membrane sample injection ion funnel ionization source based on vacuum ultraviolet photoionization technology, and combines membrane sample injection enrichment technology and ion funnel ion focusing technology, so that the detection sensitivity of the instrument is greatly improved.

The invention provides a liquid crystal biosensor for high-sensitivity visual detection of a protein marker based on functional nucleic acid regulated CRISPR-Cas12a and a preparation and detection method of the liquid crystal biosensor. The sensor comprises a ready-to-use detection reagent and a nucleic acid molecule functionalized liquid crystal detection interface, the ready-to-use detection reagent comprises the following elements or components: a Cas enzyme-crRNA compound with shearing activity and an activation sequence locking system based on a nucleic acid aptamer-activation sequence-two-dimensional material, the nucleic acid molecule functionalized liquid crystal detection interface is a liquid crystal interface modified by DNA molecules or RNA molecules, and the nucleic acid aptamer specifically recognizes target protein. The sensor is simple and convenient to operate, does not need too much manpower, is high in sensitivity, and can realize semi-quantitative or quantitative detection.

The invention discloses a label-free electrochemical aptamer sensor for detecting ochratoxin A as well as a construction method and application of the label-free electrochemical aptamer sensor. The sensor comprises a three-electrode system of a modified working electrode, a reference electrode and a counter electrode, and exonuclease I and potassium ferricyanide are contained in electrolyte; the modified working electrode is obtained by sequentially covalently immobilizing cDNA and an ochratoxin A aptamer on a VMSF modified electrode. According to the invention, potassium ferricyanide and an ochratoxin A aptamer are respectively used as an electrochemical signal molecule and a specific recognition element; a label-free electrochemical aptamer sensor is constructed by combining a signal amplification strategy of exonuclease I assisted target circulation, is used for detecting ochratoxin A in samples such as wheat samples and wine and has the characteristics of high sensitivity, wide linear range, simplicity in operation and the like.

The invention discloses a crRNA target spot and a CRISPR-Cas13a system for detecting a Hendea virus. The CRISPR-Cas13a system comprises Cas13a protein and crRNA, or a complex formed by the Cas13a protein and the crRNA; the crRNA comprises an anchoring sequence used for being combined with Cas13a protein and a guide sequence targeting a Hendea virus target sequence; the Hendea virus target sequence is located at the 632 to 659 sites of a Hendea virus genome. Experiments prove that the crRNA disclosed by the invention can realize high-sensitivity and high-specificity detection on Hendea virus nucleic acid by activating Cas13a, and the sensitivity reaches one copy (1copy / [mu] L); the method has an important application value.

The invention discloses a preparation method and application of an electrochemical biosensor based on a self-protection palindromic DNA (deoxyribonucleic acid) nanosphere. The electrochemical biosensor comprises an HP-trigger, a target miRNA (micro ribonucleic acid) and a self-protection DNA palindromic nanosphere core structure. The DNA nanosphere is of a three-dimensional spherical structure formed by four single-stranded DNAs through complementary base pairing, the three single strands of SEP-Sa, SEP-Sb and SEP-Sc contain 12 nt palindromic cohesive ends and 15 nt single-stranded arms, the single strand of HP-Sc contains 35 nt prolonged hairpin structures, the structural stability is enhanced through a self-protection mechanism of a palindromic sequence, and structural dissociation triggered by target miRNA is achieved. The target miRNA is combined with an HP-Sc hairpin structure to trigger conformational change, release complementary chains and dissociate palindromic nanospheres, and exposed DNA sites are combined with MB to generate electrochemical signals, so that a novel tool is provided for early diagnosis of tumors.

The invention provides a lead ion sensor based on a composite liquid gate transistor as well as a preparation method and application of the lead ion sensor, and belongs to the technical field of biosensors. The transistor lead ion sensor comprises a substrate, a source electrode, a drain electrode and a grid electrode, wherein the source electrode and the drain electrode are arranged on the substrate. A glassy carbon electrode is used as a grid electrode, the surface of the glassy carbon electrode is modified with a layer of nitrogen-doped graphene to serve as a base, gold nanoparticles are introduced to widen the conductivity of the glassy carbon electrode, meanwhile, the surface of a substrate material is modified with probe glutathione, the probe glutathione can be complexed with lead ions in a solution, and then the double-electric-layer characteristic between a transistor and a sample solution is changed; by measuring the Dirac point change of the device, the purpose of quantitative analysis is achieved. According to the lead ion concentration detection method, along with the change of the concentration of the target object, the lowest detection limit can reach 10 <-11 > M, the lowest response time reaches 100 s, and the sensor has high sensitivity, selectivity and stability.

The invention discloses a tumor serum exosome specific molecular marker of renal clear cell carcinoma as well as a kit and a detection reagent of the tumor serum exosome specific molecular marker. The specific molecular marker is CD147, and the specific molecular marker also comprises CD63. The expression of the CD147 in tumor cells of the renal clear cell carcinoma is obviously increased, and the content of the CD147 on serum exosomes of patients with the renal clear cell carcinoma is obviously higher than that of healthy people, bladder cancer patients and prostate cancer patients. Therefore, the compound can be used as a specific biomarker of the renal clear cell carcinoma.

The utility model provides a chromatographic column and ionization source interface for GC-IMS (gas chromatography-ion mobility spectrometry) coupling technique, which comprises a chromatographic column, an ion migration tube and a GC-IMS interface for connecting the chromatographic column and the ion migration tube, one end of the GC-IMS interface is a screw sleeved on the chromatographic column, and the other end of the GC-IMS interface is a nut fixed on the ion migration tube; the ion migration tube comprises an ionization source, a reaction area, an ion gate, a migration area and a migration tube air outlet, and the migration tube air outlet is located on the side face of the first electrode of the ion migration tube; the chromatographic column is horizontally sealed and fixed on the axis of the ion migration tube, is axially connected with the ion migration tube through a GC-IMS interface and penetrates through the ionization source, a gas outlet of the chromatographic column is positioned in the reaction region and is positioned between the ionization source and the ion gate, and eluate of the chromatographic column enters the reaction region under the driving of carrier gas for ionization analysis. And meanwhile, the eluate is quickly blown out of the ion migration tube under the action of one-way drift gas. According to the technical scheme of the utility model, the problems of GC-IMS flow matching and sample residue are solved, and the GC-IMS response time resolution is improved.

The application discloses a kind of track traffic defect detection methods based on multi-source perception and causal decoupling. By synchronously acquiring high-resolution RGB image and structural micro-vibration signal of track inspection scene, four-dimensional perception data field containing component dynamic mechanical response characteristics is constructed, and dynamic mechanical response field representing the stiffness of structure at each spatial position is generated;Instance segmentation is performed on the static topography component in the RGB image and the four-dimensional data field, and a topological relationship graph is constructed with components as nodes and functional coupling relationships as edges;According to the topological relationship graph, double abnormal reasoning at node level and edge level is performed, and combined with generative deduction based on causal decoupling and cross-modal topological consistency verification, the final comprehensive defect judgment result is generated.The application improves the recognition accuracy and robustness of track traffic defect detection on subtle defects such as micro-looseness and functional disorder.

The application discloses a kind of amoxicillin concentration detection methods based on optical fiber, using device includes: broadband light source, single-mode optical fiber, polarization controller, flow cell, TFBG-SPR sensor and spectrum analyzer.First by broadband light source to emit the light of wavelength range between 1420~1620nm, the polarization state of light is adjusted to P state by polarization controller, then through polydopamine, polyaniline and beta-lactamase layer-by-layer modified sensing probe, finally form spectrum on spectrum analyzer.Bio-identification film based on beta-lactamase modified on the surface of optical fiber will be combined with amoxicillin molecule to cause the change of refractive index, and the drift of transmission spectrum is shown on spectrum analyzer, the relationship between different amoxicillin solution concentration and transmission spectrum drift is compared, to realize the rapid, accurate, trace detection of amoxicillin concentration.The application provides a new method for the measurement of amoxicillin and other beta-lactam antibiotics concentration, which has simple structure, high accuracy and fast response, and has very important potential application value in environmental pollution and food health monitoring.

The invention belongs to the technical field of electrochemical sensors, and particularly relates to an electrochemical sensor based on cobaltosic oxide-vinegar residue derived N-doped porous carbon and application of the electrochemical sensor in dopamine detection. A specific response unit of the electrochemical sensor is a working electrode, and the working electrode is a cobaltosic oxide-vinegar residue derived N-doped porous carbon modified electrode. The cobaltosic oxide-vinegar residue derived N-doped porous carbon is obtained by in-situ growth of Co3O4 derived from a metal organic framework material ZIF-67 on a wrinkled porous self-doped nitrogen carbon-based material derived from industrial solid waste vinegar residue. The electrochemical sensor constructed by utilizing the cobaltosic oxide-vinegar residue derived N-doped porous carbon modified electrode shows good dopamine electrocatalytic activity, has a wide linear response range and good reproducibility and stability, can be used for analyzing an actual biological sample, and has a wide application prospect. And high-sensitivity real-time monitoring of dopamine can be realized.

The invention belongs to the technical field of new functional materials and biosensing detection, and particularly relates to a cascaded nano-bioreactor based on mesoporous silica and application, the cascaded nano-bioreactor based on mesoporous silica takes mesoporous silica as a carrier, and AChE, CHO and HRP are encapsulated in the mesoporous silica. AChE, CHO and HRP are fixed in mesoporous silica at the same time to construct a cascade catalysis platform, the cascade catalysis platform is used for colorimetric fluorescence dual-mode sensing detection of organophosphorus pesticides, and the cascade catalysis platform has the advantages of being easy to operate, free of precise equipment, good in detection performance, good in long-term stability and the like. The invention not only provides a new tool for high-sensitivity and convenient monitoring of pesticide residues, but also inspires the development of a high-efficiency multienzyme sensing platform, and has a wide application prospect in field analysis of the pesticide residues.

The present invention belongs to the technical field of lactic acid detection, and relates to a method for preparing a biosensor for real-time detection of saliva lactic acid and its application. A high-performance MnFe@NCNTs composite material was prepared by thermal cracking of MnFe PBA material, which greatly improved the electrochemical sensing performance. The sensor printed chip prepared based on the composite material showed excellent electrocatalytic properties and electron transfer capabilities. The preparation process of the detection chip is simple and controllable, and it is easy to achieve large-scale and productized production. The biosensor based on the chip can realize the rapid identification and dynamic response of lactic acid in real saliva samples, filling the gap of real-time detection products of saliva lactic acid on the market, and greatly improving the detection efficiency of lactic acid indicators.

The invention discloses a tungsten-based oxide composite material and a preparation method thereof, a trihydroxy dibutanone gas sensor and application, the tungsten-based oxide composite material is tungstate-tungsten oxide, and tungstate and tungsten oxide form a heterostructure; the tungsten oxide is W18O49; the tungstate is MWO4, and M is selected from at least one of Fe, Co, Ni, Cu and Sn. The successful design of the material and the preparation of the sensor provide a new thought for the detection of 3H-2B, and the epitaxial growth method can also be popularized to the fields of catalysis, energy and the like, and has extremely high reference value.

The invention discloses a cTnI electrochemical detection kit based on a nano material and SI-RAFT, a use method and application. A nano material MoS2 / CuS-Au is used as a substrate material, Apt1 is automatically assembled on an electrode through an Au-S bond, and cTnI is specifically recognized and captured. Then, the Apt2 on the Apt2-CPAD is connected to cTnI through specific recognition, a sandwich structure of Apt1-cTnI-Apt2 is formed, and then a large number of monomers with aldehyde groups are grafted to the electrode through SI-RAFT polymerization. And then Ag < + > is reduced into AgNPs by means of a silver-ammonia solution, and the AgNPs are deposited on the surface of the electrode in situ. And finally, recording the response value of the oxidation current by using the SWV to realize high-sensitivity detection of the target object. Under the optimal condition, the current intensity and the logarithm of the cTnI concentration show a good linear relationship, and the detection limit is as low as 10.83 fg.mL <-1 >. Experimental results show that the method has good selectivity and stability, is simple to operate and low in cost, and is expected to be popularized in detection and clinical application of other target objects.

The invention relates to the technical field of visual inspection, in particular to a quality inspection system for repairing surface defects of a gel finished product based on image recognition, which comprises a deformation induction imaging module for applying controllable micro-pressure deformation to a gel sample according to the projected area and hardness grade control pressure of the gel sample, capturing a dynamic sequence image of the deformation process, and outputting the dynamic sequence image; surface microcracks are detected; the characteristic analysis module is used for extracting response characteristics of a defect area from the dynamic image sequence, including distinguishing free impurities from fixed impurities according to a relative displacement track, positioning bubble defects by identifying a stress birefringence ring and detecting an opening behavior of surface microcracks in a deformation process; calculating a relaxation time constant ratio and a stress concentration factor of the defect area and the normal area, and evaluating the interface bonding strength; the repair matching module is used for determining a repair feasibility grade according to the interface bonding strength and carrying out self-adaptive correction on the process parameters; and then detecting a verification module, and comparing the variation of the stress concentration factors before and after repair to judge a repair result.

The invention discloses a biological-nano composite dissolved oxygen sensor, a preparation method thereof, and a dissolved oxygen detection system and method.The biological-nano composite dissolved oxygen sensor comprises silicon dioxide nanoparticles, the outer walls of the silicon dioxide nanoparticles are of a porous structure, and oxygen-sensitive protein is adsorbed in through holes in the outer walls of the silicon dioxide nanoparticles; a donor dye CY3 and an acceptor dye CY5 are loaded on the outer wall of the silicon dioxide nanoparticle, a hydrophilic anti-biological-pollution coating is arranged on the outer side of the silicon dioxide nanoparticle, the donor dye CY3, the acceptor dye CY5, the silicon dioxide nanoparticle and the oxygen-sensitive protein are all wrapped in the hydrophilic anti-biological-pollution coating, and the hydrophilic anti-biological-pollution coating is coated with the donor dye CY3, the acceptor dye CY5, the silicon dioxide nanoparticle and the oxygen-sensitive protein. The sensor, the detection system and the detection method can realize real-time, high-sensitivity, high-precision and low-cost detection of the dissolved oxygen of the water vapor system of the thermal equipment.

The invention relates to an electrochemical aptamer sensor and a method for detecting ochratoxin A. The electrochemical aptamer sensor comprises: a working electrode; the invention relates to a CRISPR / Cas12a reaction system. The invention also comprises a method for detecting ochratoxin A by using the electrochemical aptamer sensor. The method comprises the following steps: extracting, filtering and diluting a sample to obtain a sample solution for detection; the sample solution is added into a CRISPR / Cas12a reaction system; enabling a reaction system to be in contact with an MB-labeled single-stranded DNA modified gold electrode; and detecting the current signal change on the surface of the electrode by adopting an electrochemical method, and comparing and analyzing the detection result with the standard curve to obtain the concentration of the ochratoxin A in the to-be-detected object. According to the method, high-sensitivity detection of the micromolecular mycotoxin is realized, the detection process is simple to operate, the reaction time is short, no complex instrument is needed, and the method has good specificity and anti-interference capability and can be suitable for quantitative analysis of the ochratoxin A in a real grain sample.

A biomarker detection system based on gas generation comprises a closed container, a capillary tube and a scale mark, a magnetic bead-nucleic acid aptamer compound is used for specific recognition and capture of target protein, a platinum nanoparticle-nucleic acid aptamer compound catalyzes hydrogen peroxide to generate oxygen, a gas pressure difference is formed to push a liquid column to move, and the target protein is detected through the scale mark. The concentration of the target protein is measured by reading the length of a liquid column in a capillary tube with naked eyes, and quantitative detection is realized. The detection system improves the detection sensitivity and speed, reduces the operation complexity and cost, has high expansibility, can detect various biomarkers, and is suitable for diagnosis of various diseases such as tumors and the like. The system has the advantages of high sensitivity, rapidness, low cost and wide application prospect.

The application discloses a kind of multi-parameter wearable smart optical fiber sensing systems, the system includes: fluorescent sensing patch, for detecting target by fluorescence reaction, including biosensor, biosensor is used to react with target and generate fluorescence signal;Data glove, for real-time monitoring finger joint bending state and realizing gesture recognition, including glove and the multiple fiber bending sensors of joint part of being attached to glove, fiber bending sensor reflects finger bending degree in real time by light intensity change, and exports multidimensional light intensity signal;Wearable fiber detection meter is connected with fluorescent sensing patch, data glove, for collecting and analyzing fluorescence signal and bending signal.The multi-parameter wearable optical fiber intelligent sensing system has multi-channel, full-automatic intelligent detection and analysis capability, can simultaneously provide fluorescence, gesture bending and environmental temperature and humidity and other multi-physical and chemical parameters Real-time monitoring and analysis services provide powerful technical support for the application of each field.

The present invention provides a V2CT-based x The sensor material of MXene / In2O3 and its preparation method and application in detecting Cl2 belong to the technical field of sensor devices. The present invention mixes V2AlC with an etchant and sequentially performs ultrasonication, heating, washing and drying to obtain a first material; mixes the first material with dimethyl sulfoxide and sequentially performs stirring, washing and drying to obtain a second material; mixes the second material with a sodium hydroxide solution and sequentially performs stirring, centrifugal precipitation, washing and drying to obtain V2CT x MXene; the V2CT x MXene composite nano-In2O3 to obtain V2CT x MXene / In2O3 sensor material, resulting V2CT x The MXene / In2O3 sensor material has a high specific surface area, broadens the reaction mechanism, improves the sensitivity of the sensor, and achieves highly sensitive detection of low-concentration Cl2 gas.

This patent is based on amino-modified nano-mesoporous silicon material encapsulating methylene blue (MB), and uses a homemade negatively charged indium tin oxide (ITO) electrochemical cell to design a universal heavy metal ion electrochemical sensor. The main principle is as follows: using an aptamer that has a good effect on heavy metal ions, when it is present, it triggers the detachment of the aptamer and the nano-mesoporous silicon composite material, so that MB is released. The released MB signal molecules are captured by the negatively charged ITO electrode, so that a large number of signal molecules are gathered on the electrode surface to realize the detection of heavy metal ions. We verified the universality of the heavy metal ion electrochemical sensor designed in this patent for 6 different heavy metal ions, and then tested it for different concentrations of Cd 2+ An analytical study was conducted, and the sensor was applied to the analysis and detection of cadmium rice, providing new ideas for the development of heavy metal ion analysis research.

Sensitivity is one of important parameters for evaluating the analysis performance of the sensor. A colorimetric analysis method of a GR-5DNAzymes mediated CHA-HCR cascade amplification strategy is developed by utilizing specific binding of GR-5DNAzymes and Pb < 2 + >, so that the sensitivity of the method is improved, and the method is used for detecting Pb < 2 + >. The hemin / G quadruplex DNA enzyme is used as a signal generation element to construct a colorimetric sensing technology for high-sensitivity detection of Pb < 2 + >. According to the method, GR-5DNAzymes and Pb < 2 + > are specifically combined, a hairpin H1 is catalytically cracked, CHA circular reaction is started to form a bivalent initiation chain compound, two ends of the compound simultaneously initiate HCR reaction to form longer and more long-chain products with G quadruplex DNAzymes fragments, TMB is catalytically oxidized under the induction of H2O2 to generate blue substances, and finally the blue substances are converted into stable yellow substances under the termination action of H2SO4. According to the method, the next cascade reaction can be quickly started through a bivalent trigger chain compound formed by CHA, the detection limit is 16.71 pM, the signal amplification effect is enhanced, and some references can be provided for other multi-trigger cascade signal amplification strategies.

The invention discloses a droplet microcavity device capable of generating a micro bar code and a food detection method, the device comprises a multicolor droplet arranged on a sample, the sample is located in an emission light path of a pulse laser, an optical lens is arranged at an emission end of the pulse laser, a lens is arranged on the other side of the sample, the lens receives emission light of the sample, and the emission light of the sample is emitted by the optical lens. An optical filter and a spectrograph are sequentially arranged on the output side of the lens; the method comprises the following steps: selecting one of multi-color liquid drops, carrying out specific marking treatment, and adding into a food sample; the pulse laser emits pulse laser which is focused by the optical lens to irradiate the food sample, and emitted light of the liquid drops enters the spectrograph through the lens and the optical filter; the emission spectrum of the typical echo wall mode laser of the liquid drop is obtained; monitoring the laser signal change condition of the liquid drop in the spectrum; and determining the bit code of the liquid drop. According to the invention, the change of spectral characteristics and corresponding codes is caused by liquid drops, food detection is realized, and the detection is convenient and sensitive.

The invention discloses application of polyhydroxy cation quaternary ammonium salt magnetic beads constructed by amino epoxy ring opening in pathogenic aerosol PCR (polymerase chain reaction) enrichment detection, epoxy quaternary ammonium salt modified ferroferric oxide magnetic beads are used for enriching pathogenic microorganisms, and the application method comprises the following steps: preparing the epoxy quaternary ammonium salt modified ferroferric oxide magnetic beads into a solution with the concentration of 0.01-1mg / mL; the magnetic bead solution is added into a cyclone cup matched with a wet-wall cyclone aerosol sampler, a cyclone sampling device of the aerosol sampler is started, a detection device is started for collection, and the collected sample is pathogen aerosol. The air pathogen aerosol is efficiently adsorbed and desorbed through the polyhydroxy cation quaternary ammonium salt magnetic beads, efficient enrichment of the pathogen aerosol is achieved, then PCR high-sensitivity detection is achieved, and the method is of great importance to prevention and control of pathogen propagation.

The application relates to the field of flexible functional materials and human-computer interaction technology, and discloses a preparation method of a trehalose-enhanced anti-freezing conductive hydrogel fiber and application of the trehalose-enhanced anti-freezing conductive hydrogel fiber in intelligent gloves. The hydrogel is an acrylamide / polyvinyl alcohol double-network structure, and trehalose and lithium chloride are introduced. The trehalose forms a reversible hydrogen bond network with polymer chains through multiple hydroxyl groups, and the lithium chloride exists in the form of a hydrated ion in network pores to form an ion conductive path. The hydrogel forms a continuous linear fiber structure, the cross-sectional diameter is 0.3-2.5 mm, the flexibility is maintained at an environment of-20 DEG C, the conductivity retention rate is greater than or equal to 80%, the elongation at break is greater than or equal to 1000%, and the mechanical hysteresis is less than or equal to 5%. The application solves the problems of low-temperature failure and difficulty in fiberization of existing conductive hydrogels, and can be applied to flexible strain sensors, intelligent gloves and remote mechanical control systems.