70 results about "Complementary pair" patented technology

According to the invention, a structural domain reaction substrate sequence consisting of Exon1 (E1), P1 and Exon2 (E2) sequences of ribozyme is mutated, and E1, P1 and E2 sequences are mutated under the condition of maintaining the structural stability, so that the Azoarcus group I intron ribozyme still has enzyme activity and can maintain the capability of forming circular RNA (Ribose Nucleic Acid). The invention discloses a flexible vector construction method for preparing circular RNA (Ribonucleic Acid) without limitation of a substrate sequence, which comprises the following steps: determining a target to-be-cyclized site sequence NNUNNNN, and segmenting the target to-be-cyclized site sequence NNUNNNN into E1: NNU and E2: NNNN; with E1 and E2 sequences as references, designing IGS sequences to respectively form complementary pairing with E1 and E2; and 5'and 3 'homologous arms, IRES, CDS and other elements are respectively added. The method provided by the invention can be used for preparing the circular RNA for any target sequence, has no residual sequence, and has relatively high cyclization efficiency. The FlexCirc cyclization system designed on the basis of Azoarcus group I intron ribozyme can form the circular RNA, the cyclization substrate sequence has the characteristic of flexible design, and the cyclization efficiency can realize a relatively high cyclization proportion.

A sequence transmission method and an apparatus are provided, which may be applied to a downlink synchronization scenario, a random access scenario, a sensing scenario, a radar scenario, an integrated sensing and communication scenario, or the like, to increase sequence design diversity, and improve sequence design flexibility and target detection accuracy. The method includes: A transmit end apparatus determines N first sequences, and sends the N first sequences. An nth first sequence in the N first sequences is determined based on an nth second sequence in N second sequences, formula (I), am is a prime number, M is a positive integer greater than 1, and n=0,1, . . . , N−1. Each second sequence is a sequence in a Golay complementary pair GCP. The N second sequences include formula (II) first sub-sequence sets, each first sub-sequence set includes am second sub-sequence sets, each second sub-sequence set includes formula (III) second sequences, m=0,1, . . . , M−1, and a−1=1.

The invention relates to the field of DNA nanotechnology, and discloses a DNA origami structure based on functionalized magnetic nanoparticles, a preparation method and application, and the preparation method comprises the following steps: step 1, constructing a DNA origami structure with a cavity through a self-assembly method; the short chains in the DNA origami structure comprise a first short chain, a second short chain and a third short chain; 2, connecting a sulfydryl-functionalized first sequence to the first short chain, and chelating ferrous ions on the first sequence to obtain a sulfydryl-modified DNA origami structure; 3, ferrous ions chelated on the sulfydryl modified DNA origami structure are reduced, and a magnetic functionalized DNA origami structure internally loaded with Fe2O3 particles is obtained; and 4, connecting an antibody to a second short chain in the DNA origami structure through base complementary pairing to obtain the required DNA origami structure. According to the invention, DNA origami is combined with magnetic nanoparticles, efficient and controllable biological magnetic beads are obtained through dual immobilization and antibody modification, and a traditional application scene of DNA origami is broken through.

The invention relates to a nano groove array and a manufacturing method and application thereof, and the manufacturing method of the nano groove array comprises the following steps: taking single-stranded DNA (ssDNA) as a DNA brick, and carrying out self-assembly by adopting a complementary pairing principle of the ssDNA to obtain the nano groove array with a base and a side wall; wherein the width and the height of the nano groove array are fixed, the number of grooves is determined, and the nano groove array extends along a single direction; in the nano groove array, the width of the side wall and the width of the groove are equivalent to the diameter of two double-helix DNA (dsDNA). Compared with the prior art, the number of the grooves of the nano groove array is controllable, the width of the grooves is 4 nm, the period of the groove array is 8 nm, and the self-assembly defect can be greatly reduced.

Provided is an oligonucleotide capable of inducing the editing activity of ADAR in a cell and having excellent stability. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'-side of the first oligonucleotide, and is capable of inducing the site-specific editing of target RNA. The first oligonucleotide includes: a target-corresponding nucleotide residue; an oligonucleotide that is located on the 5'-side of the target-corresponding nucleotide residue, is complementary to the target RNA, and is composed of 3 to 6 residues; and an oligonucleotide that is located on the 3'-side of the target-corresponding nucleotide residue, is complementary to the target RNA, and is composed of 10 to 24 residues. At least one of a phosphorus-containing linking group that links a first nucleoside residue to a second nucleoside residue and a phosphorus-containing linking group that links a fourth nucleoside residue to a fifth nucleoside residue is an alkylphosphonic acid residue, an alkyl phosphate residue or a substituted phosphoramide residue, wherein the nucleoside residues are numbered from the target-corresponding nucleotide in 3' direction. The second oligonucleotide has a nucleotide residue in which a nucleotide residue corresponding to the target RNA is deleted at the 3'-end or does not form a complementary pair with the target RNA. The second oligonucleotide is composed of 2 to 10 residues, wherein a nucleotide residue other than a nucleotide residue located at the 3'-end is complementary to the target RNA.

The invention discloses a fluorescence coding probe and a single cell immunoblotting method. The nucleic acid tag comprises oligonucleotide chains f1, f2... fn, n is greater than 2, each oligonucleotide chain contains a fluorophore and a quenching group, partial sequences of the next oligonucleotide chain and the previous oligonucleotide chain are complementarily paired, and the quenching group of the next oligonucleotide chain quenches the fluorophore of the previous oligonucleotide chain. Also disclosed is a quenching tag comprising a complementary strand capable of complementary pairing with an oligonucleotide strand in a nucleic acid tag. The invention discloses a fluorescent coding probe comprising a nucleic acid label, a preparation method of the fluorescent coding probe and application of the fluorescent coding probe in a multi-target single-cell immunoblotting technology, an immunofluorescence technology and an immunohistochemical staining technology. According to the fluorescence-coded multi-target single-cell immunoblotting method disclosed by the invention, multi-target protein detection can be carried out by utilizing limited fluorescence channels, the signal loss is small, and the expansibility is high.

The present invention relates to the technical field of molecular biology, and in particular, to a sgRNA sequencing linker and use thereof. The sgRNA sequencing 3' linker sequentially comprises the following sections from a 5'-end to a 3'-end: a first non-random section, a first random section, a second non-random section, a loop-forming DNA section, and a third non-random section, wherein the first non-random section is used for being linked to the 3'-end of the sgRNA; the first random section comprises 3 to 12 basic groups; the second non-random section is reversely complementary to the third non-random section so as to form a neck ring structure in conjuncture with the loop-forming DNA section; the third non-random section is used as a primer for sgRNA reverse transcription and replication; the loop-forming DNA section is composed of a first loop-forming section and a second loop-forming section from the 5'-end to the 3'-end; the third non-random section and the second loop-forming section can be combined with the first sequencing linker primer sequence in a complementary pairing mode.

A spatio-temporal frequency multiplexing (STFM) scheme for radio frequency (RF) scanning is disclosed, in which complementary sequence pairs (or "Golay pairs") are transmitted at different times using multiple frequencies. Transmission and reception of the sequences can occur on multiple transmit (Tx) and / or receive (Rx) radio sectors to scan the distance, azimuth, elevation, and (optionally) velocity of an object in an entire area.

The invention discloses an exosome metabolic fingerprint extraction and surface protein quantification integrated detection method based on a porous magnetic nano assembly material. The preparation method comprises the following steps: firstly, preparing a porous magnetic nano assembly material Fe3O4 (at) SiO2-Apt-Au, and modifying the surface of a SiO2 shell layer with an exosome surface protein aptamer; au nanoparticles serve as an LDI-MS mass label, the surface of the Au nanoparticles is connected with an antisense strand of the aptamer, and the Au nanoparticles are connected with the Fe3O4 (at) SiO2 magnetic nanoparticles through complementary pairing with a base of the aptamer. In exosome metabolism detection, the nano assembly material can play the roles of exosome capture and LDI-MS auxiliary enhancement of a matrix; a strand displacement reaction site and an LDI-MS mass tag are provided; by combining LDI-MS as a downstream analysis tool, high-sensitivity, high-resolution, low-sample-consumption, high-throughput, rapid and low-cost exosome metabolic fingerprint extraction and surface protein quantitative analysis can be realized.

This application relates to the field of lithium-ion battery technology, and particularly to modified binders and their preparation methods, and secondary batteries. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; polyurethane, wherein nitrogen-containing heterocyclic groups are grafted into the molecular chain of the polyurethane, and these nitrogen-containing heterocyclic groups contain hydrogen bond acceptor groups and hydrogen bond donor groups; and a crosslinking agent, which contains hydrogen bond donor groups, hydrogen bond acceptor groups, and positively charged groups. The carboxyl-containing polymer binder provides basic bonding properties; the polyurethane provides flexibility and elasticity. Based on this, the polyurethane and the crosslinking agent form a complementary pairing pattern similar to hydrogen bonds in DNA and RNA, allowing the crosslinking agent to accurately recognize the nitrogen-containing heterocyclic groups on the polyurethane, resulting in high bonding strength. The crosslinking agent and the carboxyl-containing polymer binder exhibit electrostatic adsorption. The modified binder possesses high flexibility, elasticity, and adhesive properties, effectively mitigating the impact caused by the volume expansion of silicon-based anode materials.

Reduced delay and improved threshold tracking over PVT in a comparator input circuit and comparator are provided by circuits that include an inverter stage having a first complementary pair of transistors connected in a push-pull configuration. An input of the inverter stage is coupled to the gates of the first complementary pair of transistors, and an output of the inverter is coupled to the drains of the first complementary pair of transistors. The circuits also include one or more first degeneration transistors coupled between a source of one of the first complementary pair of transistors and a power supply rail of the circuit, and a reference circuit with an output coupled to one or more gates of the first degeneration transistors. The reference circuit controls the one or more first degeneration transistors to cause the inverter stage to have a threshold voltage equal to a threshold control voltage.

Provided is an oligonucleotide having excellent stability and capable of inducing ADAR editing activity in a cell. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'side thereof, and induces site-specific editing against a target RNA. The first oligonucleotide is composed of a target-corresponding nucleotide residue, an oligonucleotide having 3 to 6 residues complementary to the target RNA on the 5'side thereof, and an oligonucleotide having 10 to 24 residues complementary to the target RNA on the 3 'side thereof. And at least one of a phosphorus-containing linking group connecting the nucleoside residues at the first position and the second position and a phosphorus-containing linking group connecting the nucleoside residues at the fourth position and the fifth position is an alkyl phosphonic acid residue, an alkyl phosphate residue or a substituted phosphamide residue. The second oligonucleotide has a nucleotide residue corresponding to the target RNA deleted at the 3'terminal, or has a nucleotide residue that does not form a complementary pair with the target RNA, the number of residues being 2 to 10, and the nucleotide residues other than the 3 'terminal being complementary to the target RNA.

The invention provides a photoelectrochemical biosensor for detecting exosome lncRNA as well as a construction method and application of the photoelectrochemical biosensor. Specifically, an ITO / COF / TiO2 electrode prepared from photoelectric active material COF nanospheres and TiO2 nanospheres generates initial light current under light excitation in a visible light range. Then the electrode is assembled step by step, porous active sites of the TiO2 nanospheres on the surface of the electrode are sealed by pDNA of which the 3'end is modified with a phosphate group, and the phosphate group can ensure that the pDNA is not influenced by subsequent TdT. Then, target lncRNA competes for pDNA on the electrode through base complementary pairing to expose a site, and Tyr can be adsorbed on the surface of the electrode through the pores of the TiO2 nanospheres. TdT catalyzes dCTP, poly (C) is generated in situ on the surface of the electrode by taking a phenolic hydroxyl group of Tyr as a polymerization site, NaBH4 is used for reducing AgNO3 to generate DNA-AgNCs, and the DNA-AgNCs strengthens a photocurrent signal through an SPR (Surface Plasmon Resonance) effect. The sensor has high selectivity and high sensitivity for detecting the content of lncRNA in human plasma exosome lysate, and has huge application potential for biomarker detection in early diagnosis of lung cancer.

The application discloses an intelligent response type DNA nano device and a construction method and application thereof, and belongs to the field of DNA nanotechnology; the intelligent response type DNA nano device comprises a plurality of different DNA tetrahedron basic units and extension chains and miRNA response chains arranged on the DNA tetrahedron basic units; wherein the DNA tetrahedron basic units are connected by base complementary pairing between the extension chains and the miRNA response chains to form a multimer structure; the miRNA response chain comprises a base sequence capable of specific complementary pairing with a target miRNA to be detected, and the extension chain and the miRNA response chain are separated, so that specific configuration transformation of the nano device is induced. Through programmable assembly of the DNA tetrahedron and miRNA-induced configuration dynamic response, high specificity, high sensitivity, multi-channel parallel detection of a plurality of miRNAs are realized, and problems such as insufficient specificity, low sensitivity and poor targeting of a traditional miRNA detection method are overcome, so that the application has a wide application prospect in the fields of biomedical research and clinical diagnosis.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) visual biosensor for diagnosing coagulase negative staphylococcus. The LAMP visual biosensor comprises (1) a coagulase negative staphylococcus aptamer sequence; (2) optimizing reaction conditions of the coagulase negative staphylococcus aptamer molecular beacon fluorescent biosensor; and (3) detecting coagulase negative staphylococcus. And coupling the aptamer and the magnetic bead modified with the complementary chain through complementary base pairing to form a magnetic bead-complementary chain-aptamer compound. When the target exists, the aptamer identifies the target and dissociates from the complementary chain, and signal normalization is completed through magnetic separation. And performing amplification by using the magnetically separated supernatant as an LAMP template to realize signal amplification. In the LAMP amplification process, pyrophosphoric acid generated by decomposition of the dNTP is combined with Mg < 2 + >, and the concentration of Mg < 2 + > is reduced, so that hydroxynaphthol blue is triggered to develop, and visual output of signals is realized. The Ct value is generated based on LAMP amplification, and quantitative detection can be achieved.

A method of target enrichment or depletion from a sample with an analyte is described. A probe has one of a left-handed PNA pair and a targeting moiety, in which the left-handed PNA pair are a complementary pair of PNAs that are chiral and have a cyclic backbone modification that induces a left-handed helical structure. A capture surface has the other of the left-handed PNA pair; and the left-handed PNA pair bind to hybridize the probe to the capture surface, which may be a magnetic bead.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The invention provides a preparation and detection method of a liquid gate graphene transistor for detecting a colorectal cancer marker piR-54265, and the method adopts a one-step method to fix a PolyA15T5-single-stranded DNA biomolecular probe on the surface of a gold gate of the liquid gate graphene transistor to realize detection of a colorectal cancer target molecule piR-54265. The fixed probe and a target piR-54265 in an electrolyte solution can be subjected to base complementary pairing hybridization, due to the fact that a phosphoric acid framework of the piR-54265 is negatively charged and graphene has the bipolar characteristic, when the probe and the target are hybridized, the potential on the surface of a grid electrode changes, and by fixing the voltage of a drain electrode and the voltage of the grid electrode relative to a source electrode, the target piR-54265 can be obtained. The channel current can change in real time along with the change of the concentration of the added piR-54265, real-time detection without marks and amplification is realized, the lowest detection limit reaches 18.072 aM, the fastest detection time can reach 15 minutes, and the sensitivity is very high. Clinical sample detection is realized, clinical serum samples of colorectal cancer patients and healthy people can be effectively distinguished, and clinical application value is achieved.

A semiconductor device includes a column select circuit coupled to a complementary pair of bitlines. The column select circuit includes a pair of p-type transistors each coupled in series with a respective one of the complementary pair of bitlines and configured to provide a voltage on the respective one of the complementary pair of bitlines to a respective one of a complementary pair of local input / output (LIO) lines in response to a column select signal. The semiconductor device further includes a LIO circuit comprising a pair of transistors cross-coupled between the complementary pair of LIO lines. The pair of transistors is configured to transition the complementary pair of LIO lines to complementary values based on values provided on the complementary pair of bitlines.

This invention discloses a DNA structure for targeting and regulating EGFR dimerization on the cell membrane surface. The DNA structure is a double-stranded DNA bridge constructed using nucleic acid self-assembly. The double-stranded DNA bridge includes aptamer probes A and B, and a complementary strand C, which is complementary to aptamer probes A and B. This invention utilizes the specific recognition and binding of aptamer probes A and B to the EGFR receptor on the cell membrane, each exposing a DNA sequence. When complementary strand C is present, the exposed sequences on aptamer probes A and B can self-assemble with complementary strand C through base pairing principles, forming a double-stranded DNA bridge. By using double-stranded DNA bridges of different lengths, the distance for EGFR dimerization can be controlled. This achieves an effective spatial distance for inhibiting receptor dimerization, ultimately inhibiting cellular behavior. This provides a new approach for effectively regulating the occurrence and development of cancer and has significant implications for targeted cancer therapy.

One aspect of the utility model is a multi-phase conversion circuit comprising a primary side switching circuit. The primary side switching circuit includes a plurality of complementary pairs of transistors. The plurality of transistor complementary pairs are coupled in parallel. The multi-phase conversion circuit also comprises a resonant tank which is coupled to the primary side switching circuit and comprises a plurality of capacitors, a plurality of resonant inductors and a plurality of transformers. The multi-phase conversion circuit also includes a secondary side switching circuit coupled to the resonant tank, and the secondary side switching circuit is of a full bridge architecture. Each of a plurality of complementary transistor pairs of the primary side switching circuit includes a first transistor and a second transistor that are complementary. The first transistor and the second transistor are coupled in series. A node is arranged between the first transistor and the second transistor of each complementary pair of transistors. The node is coupled to a corresponding one of the plurality of resonant inductors of the resonant tank.

A STA receives information indicating a distribution bandwidth and a set of distributed resource units (DRUs) from a plurality of DRU allocations for the distribution bandwidth. The STA determines a first DRU long training field (LTF) sequence associated with a first DRU of the set of DRUs. The first DRU LTF includes a first component and a second component which are a first complementary sequence based on a Golay complementary pair (GCP). The STA determines a second DRU LTF sequence associated with a second DRU of the set, including a third component and at least a fourth component, which are a second complementary sequence based on the GCP. The STA transmits, to an AP, a frame including a physical layer (PHY) preamble including the first DRU LTF and the second DRU LTF, which are associated with a third DRU having a size based on the first and the second DRUs.

The invention provides a novel BiFC fluorescent complementary pair or recombinant fluorescent protein and application thereof. The self-assembly capacity of the fluorescence complementary pair or recombinant fluorescent protein is lower than that of a VN173 / VC155 fluorescence complementary pair, background fluorescence signals are remarkably reduced compared with that of the VN173 / VC155 fluorescence complementary pair, the specificity of BiFC analysis is improved, meanwhile, fluorescence signals are stronger than that of a VN210 / VC210 fluorescence complementary pair, fluorescence signals are remarkably increased compared with that of the VN210 / VC210 fluorescence complementary pair, and the sensitivity of BiFC analysis is improved.

A method for bonding a first substrate to a second substrate, where the first substrate has a set of alignment marks, and the second substrate has a set of complementary alignment marks. The set of alignment marks on the first substrate and the set of complementary alignment marks on the second substrate are utilized to determine a bonding overlay error between the first and second substrates prior to bonding. Corrections are then implemented during bonding of the first substrate to the second substrate based on the determined bonding overlay error.

The application provides an electrochemical DNA biosensor for detecting human papilloma virus 16 subtype (HPV-16), which utilizes base complementary pairing to specifically combine different single-stranded DNAs, and when HPV-16 is added, a DNA strand displacement reaction can be triggered to increase the electrochemical impedance (EIS) response. Within a certain range, as the concentration of HPV-16 increases, the EIS response signal also increases. The electrochemical DNA biosensor provided by the application has the advantages of simple preparation process, convenient operation, low detection limit of 33.8 pM and fast detection speed.

Provided is a blocking sequence, a kit thereof, and a method for using same. The blocking sequence comprises a blocking sequence 1, a blocking sequence 2, a blocking sequence 3, and a blocking sequence 4. The blocking sequence 1 is complementary to a sequencing joint P5 and used for blocking the sequencing joint P5 at the 5' terminal of a first chain when a capture library is prepared in a target area. The blocking sequence 2 is complementary to an Rspl sequence and used for blocking the Rspl sequence in a 5'-to-3' direction on the first chain when the capture library is prepared in the target area. The blocking sequence 3 is partially complementary to an Rsp2' sequence and used for blocking the Rsp2' sequence in a 5'-to-3' direction on a second chain when the capture library is prepared in the target area. The blocking sequence 4 is complementary to a sequencing joint P7' and used for blocking the sequencing joint P7' at the 5' terminal of the second chain when the capture library is prepared in the target area. The blocking sequence can promote blocking and effective complementary pairing with joint sequences, improve hybridization specificity, and significantly reduce the cross-contamination rate.

A sequence transmission method and device can be applied to scenes such as downlink synchronization, random access, perception, radar or communication perception integration, and can increase the design diversity of sequences and improve the flexibility of sequence design and the accuracy of target detection. The method comprises the following steps: a sending end device determines N first sequences and sends the N first sequences; wherein the nth first sequence in the N first sequences is determined according to the nth second sequence in the N second sequences, and the formula (I) is shown in the specification, am is a prime number, M is a positive integer greater than 1, and n is equal to 0, 1,..., N-1. Each second sequence is a sequence in a Gray Complementary Pair (GCP). The N second sequences comprise first sub-sequence sets shown in the formula (II), each first sub-sequence set comprises am second sub-sequence sets, each second sub-sequence set comprises second sequences shown in the formula (III), m = 0, 1,..., M-1, a-1 = 1. In each first subsequence set, any two adjacent second subsequence sets are the same, or second sequences with the same indexes in any two adjacent second subsequence sets form the GCP.

The present application provides a method and device for splitting a DNA sequence, the method comprising: determining a search region in a DNA sequence according to a preset oligonucleotide length; intercepting a DNA sequence in the search region according to a preset sequence length to obtain a DNA subsequence group; scoring the DNA subsequence group through a deep learning model, and determining the DNA subsequence with the highest score from the DNA subsequence group as a target sequence according to the scoring result; adjusting the number of bases of the target sequence according to the principle of similar DNA denaturation temperature to obtain a base complementary pairing region between single-stranded oligonucleotides when the DNA sequence is split; splitting the DNA sequence according to the base complementary pairing region to obtain a single-stranded oligonucleotide. Through the method provided by the present application, the base complementary pairing region with the highest score can be obtained from the DNA sequence, and the DNA sequence can be split, thereby improving the assembly efficiency of PCA assembly.

The invention discloses a preparation method and application of an electrochemical biosensor based on a self-protection palindromic DNA (deoxyribonucleic acid) nanosphere. The electrochemical biosensor comprises an HP-trigger, a target miRNA (micro ribonucleic acid) and a self-protection DNA palindromic nanosphere core structure. The DNA nanosphere is of a three-dimensional spherical structure formed by four single-stranded DNAs through complementary base pairing, the three single strands of SEP-Sa, SEP-Sb and SEP-Sc contain 12 nt palindromic cohesive ends and 15 nt single-stranded arms, the single strand of HP-Sc contains 35 nt prolonged hairpin structures, the structural stability is enhanced through a self-protection mechanism of a palindromic sequence, and structural dissociation triggered by target miRNA is achieved. The target miRNA is combined with an HP-Sc hairpin structure to trigger conformational change, release complementary chains and dissociate palindromic nanospheres, and exposed DNA sites are combined with MB to generate electrochemical signals, so that a novel tool is provided for early diagnosis of tumors.

The present application relates to the field of interface circuits, and provides an interface circuit and a chip, and an electronic device, which can enhance the ESD design window by means of circuit design. The interface circuit comprises a control circuit, a previous-stage driving circuit, and a subsequent-stage driving circuit. The control circuit is connected to the previous-stage driving circuit. The previous-stage driving circuit comprises a first output end, a second output end, a third output end, and a fourth output end. The subsequent-stage driving circuit comprises a second P-type transistor, a first P-type transistor, a first N-type transistor and a second N-type transistor which are connected between a high-level power supply line and a low-level power supply line in a complementary-symmetric manner, and gates of the four transistors are respectively connected to the first output end, the second output end, the third output end and the fourth output end. Under the control of a high pulse voltage on the high-level power supply line, the control circuit controls the second output end and the third output end of the previous-stage driving circuit to float, the first output end to output a high level, and the fourth output end to output a low level.