47 results about "Complementary pair" patented technology

A sequence transmission method and an apparatus are provided, which may be applied to a downlink synchronization scenario, a random access scenario, a sensing scenario, a radar scenario, an integrated sensing and communication scenario, or the like, to increase sequence design diversity, and improve sequence design flexibility and target detection accuracy. The method includes: A transmit end apparatus determines N first sequences, and sends the N first sequences. An nth first sequence in the N first sequences is determined based on an nth second sequence in N second sequences, formula (I), am is a prime number, M is a positive integer greater than 1, and n=0,1, . . . , N−1. Each second sequence is a sequence in a Golay complementary pair GCP. The N second sequences include formula (II) first sub-sequence sets, each first sub-sequence set includes am second sub-sequence sets, each second sub-sequence set includes formula (III) second sequences, m=0,1, . . . , M−1, and a−1=1.

The invention relates to a nano groove array and a manufacturing method and application thereof, and the manufacturing method of the nano groove array comprises the following steps: taking single-stranded DNA (ssDNA) as a DNA brick, and carrying out self-assembly by adopting a complementary pairing principle of the ssDNA to obtain the nano groove array with a base and a side wall; wherein the width and the height of the nano groove array are fixed, the number of grooves is determined, and the nano groove array extends along a single direction; in the nano groove array, the width of the side wall and the width of the groove are equivalent to the diameter of two double-helix DNA (dsDNA). Compared with the prior art, the number of the grooves of the nano groove array is controllable, the width of the grooves is 4 nm, the period of the groove array is 8 nm, and the self-assembly defect can be greatly reduced.

The invention discloses a fluorescence coding probe and a single cell immunoblotting method. The nucleic acid tag comprises oligonucleotide chains f1, f2... fn, n is greater than 2, each oligonucleotide chain contains a fluorophore and a quenching group, partial sequences of the next oligonucleotide chain and the previous oligonucleotide chain are complementarily paired, and the quenching group of the next oligonucleotide chain quenches the fluorophore of the previous oligonucleotide chain. Also disclosed is a quenching tag comprising a complementary strand capable of complementary pairing with an oligonucleotide strand in a nucleic acid tag. The invention discloses a fluorescent coding probe comprising a nucleic acid label, a preparation method of the fluorescent coding probe and application of the fluorescent coding probe in a multi-target single-cell immunoblotting technology, an immunofluorescence technology and an immunohistochemical staining technology. According to the fluorescence-coded multi-target single-cell immunoblotting method disclosed by the invention, multi-target protein detection can be carried out by utilizing limited fluorescence channels, the signal loss is small, and the expansibility is high.

A spatio-temporal frequency multiplexing (STFM) scheme for radio frequency (RF) scanning is disclosed, in which complementary sequence pairs (or "Golay pairs") are transmitted at different times using multiple frequencies. Transmission and reception of the sequences can occur on multiple transmit (Tx) and / or receive (Rx) radio sectors to scan the distance, azimuth, elevation, and (optionally) velocity of an object in an entire area.

The invention discloses an exosome metabolic fingerprint extraction and surface protein quantification integrated detection method based on a porous magnetic nano assembly material. The preparation method comprises the following steps: firstly, preparing a porous magnetic nano assembly material Fe3O4 (at) SiO2-Apt-Au, and modifying the surface of a SiO2 shell layer with an exosome surface protein aptamer; au nanoparticles serve as an LDI-MS mass label, the surface of the Au nanoparticles is connected with an antisense strand of the aptamer, and the Au nanoparticles are connected with the Fe3O4 (at) SiO2 magnetic nanoparticles through complementary pairing with a base of the aptamer. In exosome metabolism detection, the nano assembly material can play the roles of exosome capture and LDI-MS auxiliary enhancement of a matrix; a strand displacement reaction site and an LDI-MS mass tag are provided; by combining LDI-MS as a downstream analysis tool, high-sensitivity, high-resolution, low-sample-consumption, high-throughput, rapid and low-cost exosome metabolic fingerprint extraction and surface protein quantitative analysis can be realized.

This application relates to the field of lithium-ion battery technology, and particularly to modified binders and their preparation methods, and secondary batteries. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; polyurethane, wherein nitrogen-containing heterocyclic groups are grafted into the molecular chain of the polyurethane, and these nitrogen-containing heterocyclic groups contain hydrogen bond acceptor groups and hydrogen bond donor groups; and a crosslinking agent, which contains hydrogen bond donor groups, hydrogen bond acceptor groups, and positively charged groups. The carboxyl-containing polymer binder provides basic bonding properties; the polyurethane provides flexibility and elasticity. Based on this, the polyurethane and the crosslinking agent form a complementary pairing pattern similar to hydrogen bonds in DNA and RNA, allowing the crosslinking agent to accurately recognize the nitrogen-containing heterocyclic groups on the polyurethane, resulting in high bonding strength. The crosslinking agent and the carboxyl-containing polymer binder exhibit electrostatic adsorption. The modified binder possesses high flexibility, elasticity, and adhesive properties, effectively mitigating the impact caused by the volume expansion of silicon-based anode materials.

Reduced delay and improved threshold tracking over PVT in a comparator input circuit and comparator are provided by circuits that include an inverter stage having a first complementary pair of transistors connected in a push-pull configuration. An input of the inverter stage is coupled to the gates of the first complementary pair of transistors, and an output of the inverter is coupled to the drains of the first complementary pair of transistors. The circuits also include one or more first degeneration transistors coupled between a source of one of the first complementary pair of transistors and a power supply rail of the circuit, and a reference circuit with an output coupled to one or more gates of the first degeneration transistors. The reference circuit controls the one or more first degeneration transistors to cause the inverter stage to have a threshold voltage equal to a threshold control voltage.

Provided is an oligonucleotide having excellent stability and capable of inducing ADAR editing activity in a cell. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'side thereof, and induces site-specific editing against a target RNA. The first oligonucleotide is composed of a target-corresponding nucleotide residue, an oligonucleotide having 3 to 6 residues complementary to the target RNA on the 5'side thereof, and an oligonucleotide having 10 to 24 residues complementary to the target RNA on the 3 'side thereof. And at least one of a phosphorus-containing linking group connecting the nucleoside residues at the first position and the second position and a phosphorus-containing linking group connecting the nucleoside residues at the fourth position and the fifth position is an alkyl phosphonic acid residue, an alkyl phosphate residue or a substituted phosphamide residue. The second oligonucleotide has a nucleotide residue corresponding to the target RNA deleted at the 3'terminal, or has a nucleotide residue that does not form a complementary pair with the target RNA, the number of residues being 2 to 10, and the nucleotide residues other than the 3 'terminal being complementary to the target RNA.

The invention provides a photoelectrochemical biosensor for detecting exosome lncRNA as well as a construction method and application of the photoelectrochemical biosensor. Specifically, an ITO / COF / TiO2 electrode prepared from photoelectric active material COF nanospheres and TiO2 nanospheres generates initial light current under light excitation in a visible light range. Then the electrode is assembled step by step, porous active sites of the TiO2 nanospheres on the surface of the electrode are sealed by pDNA of which the 3'end is modified with a phosphate group, and the phosphate group can ensure that the pDNA is not influenced by subsequent TdT. Then, target lncRNA competes for pDNA on the electrode through base complementary pairing to expose a site, and Tyr can be adsorbed on the surface of the electrode through the pores of the TiO2 nanospheres. TdT catalyzes dCTP, poly (C) is generated in situ on the surface of the electrode by taking a phenolic hydroxyl group of Tyr as a polymerization site, NaBH4 is used for reducing AgNO3 to generate DNA-AgNCs, and the DNA-AgNCs strengthens a photocurrent signal through an SPR (Surface Plasmon Resonance) effect. The sensor has high selectivity and high sensitivity for detecting the content of lncRNA in human plasma exosome lysate, and has huge application potential for biomarker detection in early diagnosis of lung cancer.

The application discloses an intelligent response type DNA nano device and a construction method and application thereof, and belongs to the field of DNA nanotechnology; the intelligent response type DNA nano device comprises a plurality of different DNA tetrahedron basic units and extension chains and miRNA response chains arranged on the DNA tetrahedron basic units; wherein the DNA tetrahedron basic units are connected by base complementary pairing between the extension chains and the miRNA response chains to form a multimer structure; the miRNA response chain comprises a base sequence capable of specific complementary pairing with a target miRNA to be detected, and the extension chain and the miRNA response chain are separated, so that specific configuration transformation of the nano device is induced. Through programmable assembly of the DNA tetrahedron and miRNA-induced configuration dynamic response, high specificity, high sensitivity, multi-channel parallel detection of a plurality of miRNAs are realized, and problems such as insufficient specificity, low sensitivity and poor targeting of a traditional miRNA detection method are overcome, so that the application has a wide application prospect in the fields of biomedical research and clinical diagnosis.

The invention discloses an LAMP (Loop-Mediated Isothermal Amplification) visual biosensor for diagnosing coagulase negative staphylococcus. The LAMP visual biosensor comprises (1) a coagulase negative staphylococcus aptamer sequence; (2) optimizing reaction conditions of the coagulase negative staphylococcus aptamer molecular beacon fluorescent biosensor; and (3) detecting coagulase negative staphylococcus. And coupling the aptamer and the magnetic bead modified with the complementary chain through complementary base pairing to form a magnetic bead-complementary chain-aptamer compound. When the target exists, the aptamer identifies the target and dissociates from the complementary chain, and signal normalization is completed through magnetic separation. And performing amplification by using the magnetically separated supernatant as an LAMP template to realize signal amplification. In the LAMP amplification process, pyrophosphoric acid generated by decomposition of the dNTP is combined with Mg < 2 + >, and the concentration of Mg < 2 + > is reduced, so that hydroxynaphthol blue is triggered to develop, and visual output of signals is realized. The Ct value is generated based on LAMP amplification, and quantitative detection can be achieved.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The invention provides a preparation and detection method of a liquid gate graphene transistor for detecting a colorectal cancer marker piR-54265, and the method adopts a one-step method to fix a PolyA15T5-single-stranded DNA biomolecular probe on the surface of a gold gate of the liquid gate graphene transistor to realize detection of a colorectal cancer target molecule piR-54265. The fixed probe and a target piR-54265 in an electrolyte solution can be subjected to base complementary pairing hybridization, due to the fact that a phosphoric acid framework of the piR-54265 is negatively charged and graphene has the bipolar characteristic, when the probe and the target are hybridized, the potential on the surface of a grid electrode changes, and by fixing the voltage of a drain electrode and the voltage of the grid electrode relative to a source electrode, the target piR-54265 can be obtained. The channel current can change in real time along with the change of the concentration of the added piR-54265, real-time detection without marks and amplification is realized, the lowest detection limit reaches 18.072 aM, the fastest detection time can reach 15 minutes, and the sensitivity is very high. Clinical sample detection is realized, clinical serum samples of colorectal cancer patients and healthy people can be effectively distinguished, and clinical application value is achieved.

One aspect of the utility model is a multi-phase conversion circuit comprising a primary side switching circuit. The primary side switching circuit includes a plurality of complementary pairs of transistors. The plurality of transistor complementary pairs are coupled in parallel. The multi-phase conversion circuit also comprises a resonant tank which is coupled to the primary side switching circuit and comprises a plurality of capacitors, a plurality of resonant inductors and a plurality of transformers. The multi-phase conversion circuit also includes a secondary side switching circuit coupled to the resonant tank, and the secondary side switching circuit is of a full bridge architecture. Each of a plurality of complementary transistor pairs of the primary side switching circuit includes a first transistor and a second transistor that are complementary. The first transistor and the second transistor are coupled in series. A node is arranged between the first transistor and the second transistor of each complementary pair of transistors. The node is coupled to a corresponding one of the plurality of resonant inductors of the resonant tank.

A STA receives information indicating a distribution bandwidth and a set of distributed resource units (DRUs) from a plurality of DRU allocations for the distribution bandwidth. The STA determines a first DRU long training field (LTF) sequence associated with a first DRU of the set of DRUs. The first DRU LTF includes a first component and a second component which are a first complementary sequence based on a Golay complementary pair (GCP). The STA determines a second DRU LTF sequence associated with a second DRU of the set, including a third component and at least a fourth component, which are a second complementary sequence based on the GCP. The STA transmits, to an AP, a frame including a physical layer (PHY) preamble including the first DRU LTF and the second DRU LTF, which are associated with a third DRU having a size based on the first and the second DRUs.

The invention provides a novel BiFC fluorescent complementary pair or recombinant fluorescent protein and application thereof. The self-assembly capacity of the fluorescence complementary pair or recombinant fluorescent protein is lower than that of a VN173 / VC155 fluorescence complementary pair, background fluorescence signals are remarkably reduced compared with that of the VN173 / VC155 fluorescence complementary pair, the specificity of BiFC analysis is improved, meanwhile, fluorescence signals are stronger than that of a VN210 / VC210 fluorescence complementary pair, fluorescence signals are remarkably increased compared with that of the VN210 / VC210 fluorescence complementary pair, and the sensitivity of BiFC analysis is improved.

A method for bonding a first substrate to a second substrate, where the first substrate has a set of alignment marks, and the second substrate has a set of complementary alignment marks. The set of alignment marks on the first substrate and the set of complementary alignment marks on the second substrate are utilized to determine a bonding overlay error between the first and second substrates prior to bonding. Corrections are then implemented during bonding of the first substrate to the second substrate based on the determined bonding overlay error.

The application provides an electrochemical DNA biosensor for detecting human papilloma virus 16 subtype (HPV-16), which utilizes base complementary pairing to specifically combine different single-stranded DNAs, and when HPV-16 is added, a DNA strand displacement reaction can be triggered to increase the electrochemical impedance (EIS) response. Within a certain range, as the concentration of HPV-16 increases, the EIS response signal also increases. The electrochemical DNA biosensor provided by the application has the advantages of simple preparation process, convenient operation, low detection limit of 33.8 pM and fast detection speed.

A sequence transmission method and device can be applied to scenes such as downlink synchronization, random access, perception, radar or communication perception integration, and can increase the design diversity of sequences and improve the flexibility of sequence design and the accuracy of target detection. The method comprises the following steps: a sending end device determines N first sequences and sends the N first sequences; wherein the nth first sequence in the N first sequences is determined according to the nth second sequence in the N second sequences, and the formula (I) is shown in the specification, am is a prime number, M is a positive integer greater than 1, and n is equal to 0, 1,..., N-1. Each second sequence is a sequence in a Gray Complementary Pair (GCP). The N second sequences comprise first sub-sequence sets shown in the formula (II), each first sub-sequence set comprises am second sub-sequence sets, each second sub-sequence set comprises second sequences shown in the formula (III), m = 0, 1,..., M-1, a-1 = 1. In each first subsequence set, any two adjacent second subsequence sets are the same, or second sequences with the same indexes in any two adjacent second subsequence sets form the GCP.

The invention discloses a preparation method and application of an electrochemical biosensor based on a self-protection palindromic DNA (deoxyribonucleic acid) nanosphere. The electrochemical biosensor comprises an HP-trigger, a target miRNA (micro ribonucleic acid) and a self-protection DNA palindromic nanosphere core structure. The DNA nanosphere is of a three-dimensional spherical structure formed by four single-stranded DNAs through complementary base pairing, the three single strands of SEP-Sa, SEP-Sb and SEP-Sc contain 12 nt palindromic cohesive ends and 15 nt single-stranded arms, the single strand of HP-Sc contains 35 nt prolonged hairpin structures, the structural stability is enhanced through a self-protection mechanism of a palindromic sequence, and structural dissociation triggered by target miRNA is achieved. The target miRNA is combined with an HP-Sc hairpin structure to trigger conformational change, release complementary chains and dissociate palindromic nanospheres, and exposed DNA sites are combined with MB to generate electrochemical signals, so that a novel tool is provided for early diagnosis of tumors.

The present application relates to the field of interface circuits, and provides an interface circuit and a chip, and an electronic device, which can enhance the ESD design window by means of circuit design. The interface circuit comprises a control circuit, a previous-stage driving circuit, and a subsequent-stage driving circuit. The control circuit is connected to the previous-stage driving circuit. The previous-stage driving circuit comprises a first output end, a second output end, a third output end, and a fourth output end. The subsequent-stage driving circuit comprises a second P-type transistor, a first P-type transistor, a first N-type transistor and a second N-type transistor which are connected between a high-level power supply line and a low-level power supply line in a complementary-symmetric manner, and gates of the four transistors are respectively connected to the first output end, the second output end, the third output end and the fourth output end. Under the control of a high pulse voltage on the high-level power supply line, the control circuit controls the second output end and the third output end of the previous-stage driving circuit to float, the first output end to output a high level, and the fourth output end to output a low level.

The present application belongs to the technical field of biosensor, and particularly relates to a hydrogel confined light excitation ratio electrochemical sensing method for detecting AFB1; the present application introduces a hydrogel as a confined recognition site of a target AFB1, and based on base complementary pairing between an aptamer and a complementary strand, a double-stranded DNA structure is obtained to adsorb MB; when the target AFB1 exists, the MB is combined with the aptamer to be released, based on electrostatic repulsion, DNA chains are distributed on the surface of the hydrogel, and the MB is distributed in the pore diameter of the hydrogel, so that the MB signal is enhanced. With K3[Fe(CN)6] as a reference probe and the MB as a response probe, sensitive analysis of the AFB1 is realized, the detection range is 0.001-1000 ng / mL ‑1 , and the detection limit is 0.18 pg / mL.

The invention discloses a stimuli-responsive hepatic cell targeted light-controlled nano-carrier UACPG and a preparation method and application thereof, the preparation method comprises the following steps: extending the 3'end of sgRNA to synthesize sgRNAL, the extending part is an ASO base complementary pairing sequence, and then preparing Cas9 / sgRNA / ASO through DNA-RNA hybridization; the preparation method comprises the following steps: preparing Nd < 3 + >-sensitized core-shell UCNPs nanoparticles which take NaYF4: Tm / Yb / Ca as a core and NaYF4: Nd / Yb as a shell; dBCO group modification is carried out on the UAC nano particles, Cas9 / sgRNA / ASO is efficiently coupled through click chemistry, and UAC nano particles are synthesized; finally, the surfaces of the UAC nano particles are coated with galactosylated polyacetamide (PVAm-Gal), and the UACPG nano particles are obtained. Safe, efficient and high-targeting delivery of Cas9 / sgRNA / ASO in vivo and in vitro is realized; and the traceless release of ASO and Cas9 / sgRNA is realized under the irradiation of NIR, and the purposes of blocking virus replication, supplementing in a cccDNA pool and removing the existing cccDNA are achieved by targeting HBV pgRNA and HBV cccDNA respectively.

The application discloses a panel inner primer compatibility screening method and system and a storage medium. The method comprises the following steps: combining a plurality of primer pools into a primer pool to be sequenced, and sequencing the primer pool according to primer length and compatibility degree; and performing primer comparison in a multi-process sliding window comparison mode. In order to speed up the comparison process, a hash algorithm is added. The complementary pairing length, the pairing region GC content, the free energy and the annealing temperature required for complementary pairing are used as the compatibility evaluation factors, the weighted scores of various factors are calculated, the primer dimer information is recorded, and the result is output. Compared with the existing primer compatibility screening method, as the scale of the primer pool increases, the time and memory required for comparison will increase exponentially, and it is too dependent on manual comparison by researchers. The application can accurately output high-risk primer pairs in a short time. The application provides another way for manufacturing a virus panel.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

According to the cyclization assembly of the linear RNA and the self-cyclization method of the RNA chain, cyclization primitives are connected to the two ends of an RNA sequence to be cyclized, the 3'end and the 5 'end of the RNA sequence are made to be very close in space by means of complementary pairing interaction of bases between the cyclization primitives, and cyclization of target RNA is completed by connecting the 3' end and the 5 'end of the RNA sequence through RNA ligase. Compared with linear RNA, the cyclized RNA prepared by the method has higher stability.

The invention relates to the technical field of biological materials, in particular to light-operated DNA hydrogel and a preparation and application technology thereof. The light-operated DNA hydrogel comprises L-DNA, Y-DNA and C-DNA carrying DNA frame fluorescent dots, and the L-DNA carrying the DNA frame fluorescent dots is formed by complementary pairing of an extension chain of the DNA frame fluorescent dots and a suspension chain of the L-DNA; the C-DNA is a short chain composed of 6-12 basic groups, and the C-DNA is modified with a black hole quenching agent; the sticky tail ends of the C-DNA and the Y-DNA are complementary to form a sealing end; the light-operated DNA hydrogel is of a net structure formed by hybridizing a plurality of L-DNAs carrying DNA framework fluorescent dots and a plurality of Y-DNAs dissociated from C-DNA chains. The light-operated DNA hydrogel can be used for realizing multi-dimensional and programmable light-operated information writing and reading.

The invention discloses a sequence transmission method and device, which can improve sensing or detection performance. The method comprises: a transmitting end device determines N first sequences, the nth first sequence in the N first sequences being determined according to the nth second sequence in N second sequences, and the nth second sequence being determined according to the nth element in a first extended sequence. Wherein n is equal to 0, 1,..., N-1, and N is a positive integer greater than 1. A first solution of the equal power sum can be determined according to the first extension sequence, and the degree of the first solution of the equal power sum is larger than or equal to 1. The sending end device sends N1 first sequences in the N first sequences, each second sequence in N1 second sequences corresponding to the N1 first sequences is one sequence in Gray Complementary Pairs (GCP), and N1 is smaller than or equal to N. And the receiving end device receives the transmitted first signal of the N1 first sequences, and processes the first signal according to the N1 first sequences or the N1 second sequences corresponding to the N1 first sequences.

A sequence transmission method and an apparatus are provided. An example method includes: A transmit end apparatus determines N first sequences, where an nth first sequence in the N first sequences is determined based on an nth second sequence in N second sequences, the nth second sequence is determined based on an nth element in a first extension sequence, n=0, 1, . . . , N−1, N is a positive integer greater than 1, a first solution of equal sums of powers is determinable based on the first extension sequence, and a degree of the first solution of the equal sums of powers is greater than or equal to 1. The transmit end apparatus sends N1 first sequences in the N first sequences, where each of N1 second sequences corresponding to the N1 first sequences is a sequence in a Golay complementary pair GCP, and N1 is less than or equal to N.

The invention discloses a nucleic acid aptamer for specifically targeting L-tryptophan, which is characterized in that the structure of the L-tryptophan aptamer is redesigned and optimized through secondary structure prediction and simulation, and a cDNA (complementary deoxyribonucleic acid) sequence partially complementary with the L-tryptophan aptamer is provided. An L-tryptophan aptamer and a cDNA sequence respectively modify a quenching group and a fluorophore, and when bases of the two are complementary and paired to form a double-chain DNA structure, fluorescence is quenched. After an L-tryptophan target in a to-be-detected sample is recognized, competitive binding occurs between the aptamer and L-tryptophan, so that cDNA falls off from double-stranded DNA, and a fluorescence signal is recovered. The concentration of the L-tryptophan in the to-be-detected sample can be detected by associating the fluorescence signal with the concentration of the L-tryptophan. The newly designed L-tryptophan aptamer sequence has the characteristics of high affinity, high specificity, simplicity and convenience in synthesis, small difference between batches and the like, and the designed detection application has the advantages of simplicity and convenience in operation, high sensitivity, strong specificity and the like.