32 results about "Complementary pair" patented technology

A sequence transmission method and an apparatus are provided, which may be applied to a downlink synchronization scenario, a random access scenario, a sensing scenario, a radar scenario, an integrated sensing and communication scenario, or the like, to increase sequence design diversity, and improve sequence design flexibility and target detection accuracy. The method includes: A transmit end apparatus determines N first sequences, and sends the N first sequences. An nth first sequence in the N first sequences is determined based on an nth second sequence in N second sequences, formula (I), am is a prime number, M is a positive integer greater than 1, and n=0,1, . . . , N−1. Each second sequence is a sequence in a Golay complementary pair GCP. The N second sequences include formula (II) first sub-sequence sets, each first sub-sequence set includes am second sub-sequence sets, each second sub-sequence set includes formula (III) second sequences, m=0,1, . . . , M−1, and a−1=1.

The invention relates to a nano groove array and a manufacturing method and application thereof, and the manufacturing method of the nano groove array comprises the following steps: taking single-stranded DNA (ssDNA) as a DNA brick, and carrying out self-assembly by adopting a complementary pairing principle of the ssDNA to obtain the nano groove array with a base and a side wall; wherein the width and the height of the nano groove array are fixed, the number of grooves is determined, and the nano groove array extends along a single direction; in the nano groove array, the width of the side wall and the width of the groove are equivalent to the diameter of two double-helix DNA (dsDNA). Compared with the prior art, the number of the grooves of the nano groove array is controllable, the width of the grooves is 4 nm, the period of the groove array is 8 nm, and the self-assembly defect can be greatly reduced.

This application relates to the field of lithium-ion battery technology, and particularly to modified binders and their preparation methods, and secondary batteries. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; polyurethane, wherein nitrogen-containing heterocyclic groups are grafted into the molecular chain of the polyurethane, and these nitrogen-containing heterocyclic groups contain hydrogen bond acceptor groups and hydrogen bond donor groups; and a crosslinking agent, which contains hydrogen bond donor groups, hydrogen bond acceptor groups, and positively charged groups. The carboxyl-containing polymer binder provides basic bonding properties; the polyurethane provides flexibility and elasticity. Based on this, the polyurethane and the crosslinking agent form a complementary pairing pattern similar to hydrogen bonds in DNA and RNA, allowing the crosslinking agent to accurately recognize the nitrogen-containing heterocyclic groups on the polyurethane, resulting in high bonding strength. The crosslinking agent and the carboxyl-containing polymer binder exhibit electrostatic adsorption. The modified binder possesses high flexibility, elasticity, and adhesive properties, effectively mitigating the impact caused by the volume expansion of silicon-based anode materials.

Provided is an oligonucleotide having excellent stability and capable of inducing ADAR editing activity in a cell. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'side thereof, and induces site-specific editing against a target RNA. The first oligonucleotide is composed of a target-corresponding nucleotide residue, an oligonucleotide having 3 to 6 residues complementary to the target RNA on the 5'side thereof, and an oligonucleotide having 10 to 24 residues complementary to the target RNA on the 3 'side thereof. And at least one of a phosphorus-containing linking group connecting the nucleoside residues at the first position and the second position and a phosphorus-containing linking group connecting the nucleoside residues at the fourth position and the fifth position is an alkyl phosphonic acid residue, an alkyl phosphate residue or a substituted phosphamide residue. The second oligonucleotide has a nucleotide residue corresponding to the target RNA deleted at the 3'terminal, or has a nucleotide residue that does not form a complementary pair with the target RNA, the number of residues being 2 to 10, and the nucleotide residues other than the 3 'terminal being complementary to the target RNA.

The application discloses an intelligent response type DNA nano device and a construction method and application thereof, and belongs to the field of DNA nanotechnology; the intelligent response type DNA nano device comprises a plurality of different DNA tetrahedron basic units and extension chains and miRNA response chains arranged on the DNA tetrahedron basic units; wherein the DNA tetrahedron basic units are connected by base complementary pairing between the extension chains and the miRNA response chains to form a multimer structure; the miRNA response chain comprises a base sequence capable of specific complementary pairing with a target miRNA to be detected, and the extension chain and the miRNA response chain are separated, so that specific configuration transformation of the nano device is induced. Through programmable assembly of the DNA tetrahedron and miRNA-induced configuration dynamic response, high specificity, high sensitivity, multi-channel parallel detection of a plurality of miRNAs are realized, and problems such as insufficient specificity, low sensitivity and poor targeting of a traditional miRNA detection method are overcome, so that the application has a wide application prospect in the fields of biomedical research and clinical diagnosis.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The invention provides a preparation and detection method of a liquid gate graphene transistor for detecting a colorectal cancer marker piR-54265, and the method adopts a one-step method to fix a PolyA15T5-single-stranded DNA biomolecular probe on the surface of a gold gate of the liquid gate graphene transistor to realize detection of a colorectal cancer target molecule piR-54265. The fixed probe and a target piR-54265 in an electrolyte solution can be subjected to base complementary pairing hybridization, due to the fact that a phosphoric acid framework of the piR-54265 is negatively charged and graphene has the bipolar characteristic, when the probe and the target are hybridized, the potential on the surface of a grid electrode changes, and by fixing the voltage of a drain electrode and the voltage of the grid electrode relative to a source electrode, the target piR-54265 can be obtained. The channel current can change in real time along with the change of the concentration of the added piR-54265, real-time detection without marks and amplification is realized, the lowest detection limit reaches 18.072 aM, the fastest detection time can reach 15 minutes, and the sensitivity is very high. Clinical sample detection is realized, clinical serum samples of colorectal cancer patients and healthy people can be effectively distinguished, and clinical application value is achieved.

One aspect of the utility model is a multi-phase conversion circuit comprising a primary side switching circuit. The primary side switching circuit includes a plurality of complementary pairs of transistors. The plurality of transistor complementary pairs are coupled in parallel. The multi-phase conversion circuit also comprises a resonant tank which is coupled to the primary side switching circuit and comprises a plurality of capacitors, a plurality of resonant inductors and a plurality of transformers. The multi-phase conversion circuit also includes a secondary side switching circuit coupled to the resonant tank, and the secondary side switching circuit is of a full bridge architecture. Each of a plurality of complementary transistor pairs of the primary side switching circuit includes a first transistor and a second transistor that are complementary. The first transistor and the second transistor are coupled in series. A node is arranged between the first transistor and the second transistor of each complementary pair of transistors. The node is coupled to a corresponding one of the plurality of resonant inductors of the resonant tank.

A STA receives information indicating a distribution bandwidth and a set of distributed resource units (DRUs) from a plurality of DRU allocations for the distribution bandwidth. The STA determines a first DRU long training field (LTF) sequence associated with a first DRU of the set of DRUs. The first DRU LTF includes a first component and a second component which are a first complementary sequence based on a Golay complementary pair (GCP). The STA determines a second DRU LTF sequence associated with a second DRU of the set, including a third component and at least a fourth component, which are a second complementary sequence based on the GCP. The STA transmits, to an AP, a frame including a physical layer (PHY) preamble including the first DRU LTF and the second DRU LTF, which are associated with a third DRU having a size based on the first and the second DRUs.

A method for bonding a first substrate to a second substrate, where the first substrate has a set of alignment marks, and the second substrate has a set of complementary alignment marks. The set of alignment marks on the first substrate and the set of complementary alignment marks on the second substrate are utilized to determine a bonding overlay error between the first and second substrates prior to bonding. Corrections are then implemented during bonding of the first substrate to the second substrate based on the determined bonding overlay error.

The application provides an electrochemical DNA biosensor for detecting human papilloma virus 16 subtype (HPV-16), which utilizes base complementary pairing to specifically combine different single-stranded DNAs, and when HPV-16 is added, a DNA strand displacement reaction can be triggered to increase the electrochemical impedance (EIS) response. Within a certain range, as the concentration of HPV-16 increases, the EIS response signal also increases. The electrochemical DNA biosensor provided by the application has the advantages of simple preparation process, convenient operation, low detection limit of 33.8 pM and fast detection speed.

The invention discloses a preparation method and application of an electrochemical biosensor based on a self-protection palindromic DNA (deoxyribonucleic acid) nanosphere. The electrochemical biosensor comprises an HP-trigger, a target miRNA (micro ribonucleic acid) and a self-protection DNA palindromic nanosphere core structure. The DNA nanosphere is of a three-dimensional spherical structure formed by four single-stranded DNAs through complementary base pairing, the three single strands of SEP-Sa, SEP-Sb and SEP-Sc contain 12 nt palindromic cohesive ends and 15 nt single-stranded arms, the single strand of HP-Sc contains 35 nt prolonged hairpin structures, the structural stability is enhanced through a self-protection mechanism of a palindromic sequence, and structural dissociation triggered by target miRNA is achieved. The target miRNA is combined with an HP-Sc hairpin structure to trigger conformational change, release complementary chains and dissociate palindromic nanospheres, and exposed DNA sites are combined with MB to generate electrochemical signals, so that a novel tool is provided for early diagnosis of tumors.

The present application relates to the field of interface circuits, and provides an interface circuit and a chip, and an electronic device, which can enhance the ESD design window by means of circuit design. The interface circuit comprises a control circuit, a previous-stage driving circuit, and a subsequent-stage driving circuit. The control circuit is connected to the previous-stage driving circuit. The previous-stage driving circuit comprises a first output end, a second output end, a third output end, and a fourth output end. The subsequent-stage driving circuit comprises a second P-type transistor, a first P-type transistor, a first N-type transistor and a second N-type transistor which are connected between a high-level power supply line and a low-level power supply line in a complementary-symmetric manner, and gates of the four transistors are respectively connected to the first output end, the second output end, the third output end and the fourth output end. Under the control of a high pulse voltage on the high-level power supply line, the control circuit controls the second output end and the third output end of the previous-stage driving circuit to float, the first output end to output a high level, and the fourth output end to output a low level.

The present application belongs to the technical field of biosensor, and particularly relates to a hydrogel confined light excitation ratio electrochemical sensing method for detecting AFB1; the present application introduces a hydrogel as a confined recognition site of a target AFB1, and based on base complementary pairing between an aptamer and a complementary strand, a double-stranded DNA structure is obtained to adsorb MB; when the target AFB1 exists, the MB is combined with the aptamer to be released, based on electrostatic repulsion, DNA chains are distributed on the surface of the hydrogel, and the MB is distributed in the pore diameter of the hydrogel, so that the MB signal is enhanced. With K3[Fe(CN)6] as a reference probe and the MB as a response probe, sensitive analysis of the AFB1 is realized, the detection range is 0.001-1000 ng / mL ‑1 , and the detection limit is 0.18 pg / mL.

The application discloses a panel inner primer compatibility screening method and system and a storage medium. The method comprises the following steps: combining a plurality of primer pools into a primer pool to be sequenced, and sequencing the primer pool according to primer length and compatibility degree; and performing primer comparison in a multi-process sliding window comparison mode. In order to speed up the comparison process, a hash algorithm is added. The complementary pairing length, the pairing region GC content, the free energy and the annealing temperature required for complementary pairing are used as the compatibility evaluation factors, the weighted scores of various factors are calculated, the primer dimer information is recorded, and the result is output. Compared with the existing primer compatibility screening method, as the scale of the primer pool increases, the time and memory required for comparison will increase exponentially, and it is too dependent on manual comparison by researchers. The application can accurately output high-risk primer pairs in a short time. The application provides another way for manufacturing a virus panel.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

According to the cyclization assembly of the linear RNA and the self-cyclization method of the RNA chain, cyclization primitives are connected to the two ends of an RNA sequence to be cyclized, the 3'end and the 5 'end of the RNA sequence are made to be very close in space by means of complementary pairing interaction of bases between the cyclization primitives, and cyclization of target RNA is completed by connecting the 3' end and the 5 'end of the RNA sequence through RNA ligase. Compared with linear RNA, the cyclized RNA prepared by the method has higher stability.

The invention relates to the technical field of biological materials, in particular to light-operated DNA hydrogel and a preparation and application technology thereof. The light-operated DNA hydrogel comprises L-DNA, Y-DNA and C-DNA carrying DNA frame fluorescent dots, and the L-DNA carrying the DNA frame fluorescent dots is formed by complementary pairing of an extension chain of the DNA frame fluorescent dots and a suspension chain of the L-DNA; the C-DNA is a short chain composed of 6-12 basic groups, and the C-DNA is modified with a black hole quenching agent; the sticky tail ends of the C-DNA and the Y-DNA are complementary to form a sealing end; the light-operated DNA hydrogel is of a net structure formed by hybridizing a plurality of L-DNAs carrying DNA framework fluorescent dots and a plurality of Y-DNAs dissociated from C-DNA chains. The light-operated DNA hydrogel can be used for realizing multi-dimensional and programmable light-operated information writing and reading.

The invention discloses a sequence transmission method and device, which can improve sensing or detection performance. The method comprises: a transmitting end device determines N first sequences, the nth first sequence in the N first sequences being determined according to the nth second sequence in N second sequences, and the nth second sequence being determined according to the nth element in a first extended sequence. Wherein n is equal to 0, 1,..., N-1, and N is a positive integer greater than 1. A first solution of the equal power sum can be determined according to the first extension sequence, and the degree of the first solution of the equal power sum is larger than or equal to 1. The sending end device sends N1 first sequences in the N first sequences, each second sequence in N1 second sequences corresponding to the N1 first sequences is one sequence in Gray Complementary Pairs (GCP), and N1 is smaller than or equal to N. And the receiving end device receives the transmitted first signal of the N1 first sequences, and processes the first signal according to the N1 first sequences or the N1 second sequences corresponding to the N1 first sequences.

A sequence transmission method and an apparatus are provided. An example method includes: A transmit end apparatus determines N first sequences, where an nth first sequence in the N first sequences is determined based on an nth second sequence in N second sequences, the nth second sequence is determined based on an nth element in a first extension sequence, n=0, 1, . . . , N−1, N is a positive integer greater than 1, a first solution of equal sums of powers is determinable based on the first extension sequence, and a degree of the first solution of the equal sums of powers is greater than or equal to 1. The transmit end apparatus sends N1 first sequences in the N first sequences, where each of N1 second sequences corresponding to the N1 first sequences is a sequence in a Golay complementary pair GCP, and N1 is less than or equal to N.

The invention discloses a nucleic acid aptamer for specifically targeting L-tryptophan, which is characterized in that the structure of the L-tryptophan aptamer is redesigned and optimized through secondary structure prediction and simulation, and a cDNA (complementary deoxyribonucleic acid) sequence partially complementary with the L-tryptophan aptamer is provided. An L-tryptophan aptamer and a cDNA sequence respectively modify a quenching group and a fluorophore, and when bases of the two are complementary and paired to form a double-chain DNA structure, fluorescence is quenched. After an L-tryptophan target in a to-be-detected sample is recognized, competitive binding occurs between the aptamer and L-tryptophan, so that cDNA falls off from double-stranded DNA, and a fluorescence signal is recovered. The concentration of the L-tryptophan in the to-be-detected sample can be detected by associating the fluorescence signal with the concentration of the L-tryptophan. The newly designed L-tryptophan aptamer sequence has the characteristics of high affinity, high specificity, simplicity and convenience in synthesis, small difference between batches and the like, and the designed detection application has the advantages of simplicity and convenience in operation, high sensitivity, strong specificity and the like.

The invention relates to the technical field of lithium ion batteries, in particular to a modified binder, a preparation method thereof and a secondary battery. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; a nitrogen-containing heterocyclic group is grafted in a molecular chain of the polyurethane, and the nitrogen-containing heterocyclic group contains a hydrogen bond acceptor group and a hydrogen bond donor group; the cross-linking agent contains a hydrogen bond donor group, a hydrogen bond acceptor group and a positive charge group. The carboxyl-containing polymer binder can provide basic bonding performance; and the polyurethane can exert the flexibility and elasticity. On the basis, the polyurethane and the cross-linking agent form a hydrogen bond complementary pairing mode similar to DNA and RNA, so that the cross-linking agent can accurately recognize nitrogen-containing heterocyclic ring groups on the polyurethane, and the bonding strength is high. And the cross-linking agent and the carboxyl-containing polymer binder generate an electrostatic adsorption effect. The modified binder has high flexibility, elasticity and bonding performance, and effectively relieves impact caused by volume expansion of the silicon-based negative electrode material.

The present invention discloses a method for simultaneously detecting Pb 2+ 、Cd 2+ The invention discloses a fluorescent aptamer sensor and its preparation and detection method, wherein the magnetic beads MBs and the substrate chain S-DNA are formed into MBs@S-DNA through biotin and streptavidin, the E-DNA and the lead ion Apt on the S-DNA are subjected to base complementary pairing to form E-DNA@Apt, and finally the S-DNA in MBs@S-DNA is combined with the E-DNA and the cadmium aptamer Apt in the E-DNA@Apt through partial base complementary pairing to finally form a Y-shaped DNA secondary structure, thus preparing a fluorescent aptamer sensor. In the fluorescent aptamer sensor of the present invention, MBs is used to reduce the background signal, and Pb 2+ substrate chain, E‑DNA and Cd 2+ The introduction of aptamers ensures the specificity of the sensor and prevents cross-reaction. FAM and Cy5 are used as signal indicators to construct the MBs@S-DNA / E-DNA / Apt fluorescent aptamer sensor to simultaneously detect Pb 2+ and Cd 2+ purpose.

A linear RNA cyclization component and a method for RNA chain cyclization. Ring forming primitives are linked to two ends of an RNA sequence to be undergone ring forming, and depending on the base complementary pairing interaction between the ring forming primitives, the 3' and 5' ends of the RNA sequence are extremely close in space, and are linked by means of an RNA ligase to complete cyclization of a target RNA. The cyclized RNA prepared by the method has higher stability than a linear RNA.

Reduced delay and improved threshold tracking over PVT in a comparator input circuit and comparator are provided by circuits that include an inverter stage having a first complementary pair of transistors connected in a push-pull configuration. An input of the inverter stage is coupled to the gates of the first complementary pair of transistors, and an output of the inverter is coupled to the drains of the first complementary pair of transistors. The circuits also include one or more first degeneration transistors coupled between a source of one of the first complementary pair of transistors and a power supply rail of the circuit, and a reference circuit with an output coupled to one or more gates of the first degeneration transistors. The reference circuit controls the one or more first degeneration transistors to cause the inverter stage to have a threshold voltage equal to a threshold control voltage.

The application discloses a nucleic acid multicolor fluorescent probe and application thereof in preparation and in-situ real-time observation of sequential activation of a P53-mediated cell apoptosis pathway. A P53 fixed chain, a Bax fixed chain and a Cyt c fixed chain are modified on the surface of S-AuNSs@SiO2 through an amide bond, and a complementary chain modified with different fluorescent groups, an aptamer chain are connected through a complementary pairing rule to construct a multicolor fluorescent probe. In the presence of a target, DNA double strands are forced to be opened, and a fluorescent group is released from the system to restore quenched fluorescence. Due to 20 symmetrical 'hot spots', a stronger local electric field can be generated, and the probe based on S-AuNSs shows better optical performance. The S-AuNSs@SiO2-P of the application has high integration, is easy to obtain, and realizes in-situ real-time observation of sequential activation of a P53-mediated apoptosis pathway in living cells, and has application prospects in mechanism research, drug discovery, dynamic monitoring of an apoptosis process and the like.

The application discloses a three-dimensional DNA nano-pattern surface-enhanced Raman substrate and a preparation method thereof. The three-dimensional DNA nano-pattern is built by a DNA brick LEGO type pairing method, and metal nanoparticles are closely and orderly inlaid in the hole structure of the DNA nano-pattern. The DNA nano-pattern is built by a plurality of DNA bricks through the LEGO type pairing method, and the structure is stable and firm. When the nanoparticles are inlaid on the pattern surface through base complementary pairing with the DNA pattern, the strong repulsive force between the nanoparticles can be overcome, and the nanoparticles with a larger size can be closely and firmly inlaid on the nano-pattern surface. The three-dimensional hole sizes are different due to the different sequences of the DNA bricks, and the fixing of the nanoparticles with different diameters can be realized. The longitudinal and transverse positions of the preset DNA nano-pattern hole on the pattern surface are adopted to realize the orderly arrangement of the nanoparticles. The SERS substrate with closely contacted and orderly arranged nanoparticles is obtained through the above method. The SERS substrate provided by the application can generate high-intensity and dense and uniform Raman hot spots, and can realize high-sensitivity and high-reproducibility Raman spectrum detection.

The invention relates to a self-assembly preparation method of a sub-10-nanometer spacing electrode pair, which comprises the following steps: by taking DNA two-dimensional rectangular origami as a DNA template, respectively extending two groups of single-stranded DNA, namely extension chains, on the surface of the DNA template, respectively taking the two groups of extension chains as two connecting sites, respectively modifying gold nanorods by adopting two pairing chains to obtain gold nanorods modified by the two pairing chains, the two pairing chains are sulfydryl modified single-stranded DNA matched with the connecting sites respectively, and accurate directional arrangement of the gold nanorods on a DNA template is achieved by means of the complementary base pairing principle of DNA. Compared with the prior art, the method has the advantages that the distance between the electrode pairs is stabilized at the sub-10 nanometer scale, complicated photoetching equipment is not needed, the process flow is simple, the cost is relatively low, and a technical basis for batch preparation is provided for the development of the sub-10 nanometer electrode pairs of nanometer electronic devices.

Provided is an oligonucleotide capable of inducing the editing activity of ADAR in a cell and having excellent stability. The oligonucleotide includes a first oligonucleotide and a second oligonucleotide linked to the 5' side of the first oligonucleotide, and is capable of inducing the site-specific editing in the target RNA. The first oligonucleotide is composed of a target-corresponding nucleoside residue corresponding to an adenosine residue in the target RNA, an oligonucleotide containing 3 to 6 residues, linked to the 5' side of the target-corresponding nucleoside residue, and having a base sequence complementary to the target RNA and an oligonucleotide containing 10 to 24 residues, linked to the 3' side of the target-corresponding nucleoside residue, and having a base sequence complementary to the target RNA, and at least one phosphorus-containing linking group selected from the group consisting of a phosphorus-containing linking group linking a 1st nucleoside residue and a 2nd nucleoside residue and a phosphorus-containing linking group linking a 4th nucleoside residue and a 5th nucleoside residue, counting in the 3' direction from the target-corresponding nucleoside residue, is at least one selected from the group consisting of an alkylphosphonic residue, an alkyl phosphate residue, and a substituted phosphoramide residue. At the 3' end of the second oligonucleotide, a nucleoside residue corresponding to a nucleoside residue of the target RNA is deleted, or, at the 3' end, the second oligonucleotide has a nucleoside residue that does not form a complementary pair with the nucleoside residue of the target RNA, and contains 2 to 10 residues, and nucleoside residues at least other than at the 3' end are complementary to the target RNA.

The invention relates to a method for activating CRISPR-Cas enzyme through hot start and application of the method in nucleic acid detection, and belongs to the technical field of gene detection. The invention provides a method for controlling the enzyme activity of Cas in a CRISPR-Cas system through temperature, which comprises the following steps: adding single-stranded DNA (deoxyribonucleic acid) into the CRISPR-Cas system, inhibiting the enzyme activity of the Cas under the condition of temperature 1, and activating the enzyme activity of the Cas under the condition of temperature 2; under the condition of the temperature 1, the single-chain DNA is complementarily paired with a hand and / or spacer region base in the crRNA to form closed crRNA; the closed crRNA is thermally denatured into a single chain under the condition of the temperature 2. The Cas enzyme activity can be started by controlling the temperature, and accurate time sequence regulation and control of amplification in a single tube and CRISPR reaction are achieved. Operation is simple and equipment dependence is low. The cost is low; and the design difficulty is low.