19 results about "Complementary pair" patented technology

This application relates to the field of lithium-ion battery technology, and particularly to modified binders and their preparation methods, and secondary batteries. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; polyurethane, wherein nitrogen-containing heterocyclic groups are grafted into the molecular chain of the polyurethane, and these nitrogen-containing heterocyclic groups contain hydrogen bond acceptor groups and hydrogen bond donor groups; and a crosslinking agent, which contains hydrogen bond donor groups, hydrogen bond acceptor groups, and positively charged groups. The carboxyl-containing polymer binder provides basic bonding properties; the polyurethane provides flexibility and elasticity. Based on this, the polyurethane and the crosslinking agent form a complementary pairing pattern similar to hydrogen bonds in DNA and RNA, allowing the crosslinking agent to accurately recognize the nitrogen-containing heterocyclic groups on the polyurethane, resulting in high bonding strength. The crosslinking agent and the carboxyl-containing polymer binder exhibit electrostatic adsorption. The modified binder possesses high flexibility, elasticity, and adhesive properties, effectively mitigating the impact caused by the volume expansion of silicon-based anode materials.

Provided is an oligonucleotide having excellent stability and capable of inducing ADAR editing activity in a cell. The oligonucleotide comprises a first oligonucleotide and a second oligonucleotide linked to the 5'side thereof, and induces site-specific editing against a target RNA. The first oligonucleotide is composed of a target-corresponding nucleotide residue, an oligonucleotide having 3 to 6 residues complementary to the target RNA on the 5'side thereof, and an oligonucleotide having 10 to 24 residues complementary to the target RNA on the 3 'side thereof. And at least one of a phosphorus-containing linking group connecting the nucleoside residues at the first position and the second position and a phosphorus-containing linking group connecting the nucleoside residues at the fourth position and the fifth position is an alkyl phosphonic acid residue, an alkyl phosphate residue or a substituted phosphamide residue. The second oligonucleotide has a nucleotide residue corresponding to the target RNA deleted at the 3'terminal, or has a nucleotide residue that does not form a complementary pair with the target RNA, the number of residues being 2 to 10, and the nucleotide residues other than the 3 'terminal being complementary to the target RNA.

The application discloses a kind of lncRNA subcellular localization prediction method based on lightGBM, comprising, first, the nucleotide of the front section multiple of known lncRNA sequence is intercepted as sequence sample one;Then by based on single strand multiclass position-specific three nucleotide bias and reverse complementary kmer, sequence sample one is respectively characterized coding, the combination of two kinds of feature coding is vector;Use lightGMB as learning algorithm;Using 5-fold cross validation optimization reverse complementary kmer and LightGBM's hyperparameter;Most intercept unknown lncRNA sequence front end multiple bases as sequence sample two, and its single strand multiclass position-specific three nucleotide bias and the combination of optimized reverse complementary kmer feature coding is input in trained lightGBM, will obtain its localization subcellular type, the patent of the application can be according to long-chain non-coding RNA sequence prediction in cytoplasm, nucleus, ribosome, cytosol, exosome five subcellular positions, the application realizes simply, and prediction precision is high.

The invention provides a preparation and detection method of a liquid gate graphene transistor for detecting a colorectal cancer marker piR-54265, and the method adopts a one-step method to fix a PolyA15T5-single-stranded DNA biomolecular probe on the surface of a gold gate of the liquid gate graphene transistor to realize detection of a colorectal cancer target molecule piR-54265. The fixed probe and a target piR-54265 in an electrolyte solution can be subjected to base complementary pairing hybridization, due to the fact that a phosphoric acid framework of the piR-54265 is negatively charged and graphene has the bipolar characteristic, when the probe and the target are hybridized, the potential on the surface of a grid electrode changes, and by fixing the voltage of a drain electrode and the voltage of the grid electrode relative to a source electrode, the target piR-54265 can be obtained. The channel current can change in real time along with the change of the concentration of the added piR-54265, real-time detection without marks and amplification is realized, the lowest detection limit reaches 18.072 aM, the fastest detection time can reach 15 minutes, and the sensitivity is very high. Clinical sample detection is realized, clinical serum samples of colorectal cancer patients and healthy people can be effectively distinguished, and clinical application value is achieved.

One aspect of the utility model is a multi-phase conversion circuit comprising a primary side switching circuit. The primary side switching circuit includes a plurality of complementary pairs of transistors. The plurality of transistor complementary pairs are coupled in parallel. The multi-phase conversion circuit also comprises a resonant tank which is coupled to the primary side switching circuit and comprises a plurality of capacitors, a plurality of resonant inductors and a plurality of transformers. The multi-phase conversion circuit also includes a secondary side switching circuit coupled to the resonant tank, and the secondary side switching circuit is of a full bridge architecture. Each of a plurality of complementary transistor pairs of the primary side switching circuit includes a first transistor and a second transistor that are complementary. The first transistor and the second transistor are coupled in series. A node is arranged between the first transistor and the second transistor of each complementary pair of transistors. The node is coupled to a corresponding one of the plurality of resonant inductors of the resonant tank.

A STA receives information indicating a distribution bandwidth and a set of distributed resource units (DRUs) from a plurality of DRU allocations for the distribution bandwidth. The STA determines a first DRU long training field (LTF) sequence associated with a first DRU of the set of DRUs. The first DRU LTF includes a first component and a second component which are a first complementary sequence based on a Golay complementary pair (GCP). The STA determines a second DRU LTF sequence associated with a second DRU of the set, including a third component and at least a fourth component, which are a second complementary sequence based on the GCP. The STA transmits, to an AP, a frame including a physical layer (PHY) preamble including the first DRU LTF and the second DRU LTF, which are associated with a third DRU having a size based on the first and the second DRUs.

The application provides an electrochemical DNA biosensor for detecting human papilloma virus 16 subtype (HPV-16), which utilizes base complementary pairing to specifically combine different single-stranded DNAs, and when HPV-16 is added, a DNA strand displacement reaction can be triggered to increase the electrochemical impedance (EIS) response. Within a certain range, as the concentration of HPV-16 increases, the EIS response signal also increases. The electrochemical DNA biosensor provided by the application has the advantages of simple preparation process, convenient operation, low detection limit of 33.8 pM and fast detection speed.

The invention discloses a preparation method and application of an electrochemical biosensor based on a self-protection palindromic DNA (deoxyribonucleic acid) nanosphere. The electrochemical biosensor comprises an HP-trigger, a target miRNA (micro ribonucleic acid) and a self-protection DNA palindromic nanosphere core structure. The DNA nanosphere is of a three-dimensional spherical structure formed by four single-stranded DNAs through complementary base pairing, the three single strands of SEP-Sa, SEP-Sb and SEP-Sc contain 12 nt palindromic cohesive ends and 15 nt single-stranded arms, the single strand of HP-Sc contains 35 nt prolonged hairpin structures, the structural stability is enhanced through a self-protection mechanism of a palindromic sequence, and structural dissociation triggered by target miRNA is achieved. The target miRNA is combined with an HP-Sc hairpin structure to trigger conformational change, release complementary chains and dissociate palindromic nanospheres, and exposed DNA sites are combined with MB to generate electrochemical signals, so that a novel tool is provided for early diagnosis of tumors.

The present application relates to the field of interface circuits, and provides an interface circuit and a chip, and an electronic device, which can enhance the ESD design window by means of circuit design. The interface circuit comprises a control circuit, a previous-stage driving circuit, and a subsequent-stage driving circuit. The control circuit is connected to the previous-stage driving circuit. The previous-stage driving circuit comprises a first output end, a second output end, a third output end, and a fourth output end. The subsequent-stage driving circuit comprises a second P-type transistor, a first P-type transistor, a first N-type transistor and a second N-type transistor which are connected between a high-level power supply line and a low-level power supply line in a complementary-symmetric manner, and gates of the four transistors are respectively connected to the first output end, the second output end, the third output end and the fourth output end. Under the control of a high pulse voltage on the high-level power supply line, the control circuit controls the second output end and the third output end of the previous-stage driving circuit to float, the first output end to output a high level, and the fourth output end to output a low level.

The present application belongs to the technical field of biosensor, and particularly relates to a hydrogel confined light excitation ratio electrochemical sensing method for detecting AFB1; the present application introduces a hydrogel as a confined recognition site of a target AFB1, and based on base complementary pairing between an aptamer and a complementary strand, a double-stranded DNA structure is obtained to adsorb MB; when the target AFB1 exists, the MB is combined with the aptamer to be released, based on electrostatic repulsion, DNA chains are distributed on the surface of the hydrogel, and the MB is distributed in the pore diameter of the hydrogel, so that the MB signal is enhanced. With K3[Fe(CN)6] as a reference probe and the MB as a response probe, sensitive analysis of the AFB1 is realized, the detection range is 0.001-1000 ng / mL ‑1 , and the detection limit is 0.18 pg / mL.

This invention discloses a metal nanoparticle reagent kit, its preparation method, and its application in the detection of eczema biomarkers. The metal nanoparticle reagent kit utilizes a chemical self-assembly method to deposit metal nanoparticles onto the surface of the kit's well plates. ADNA is modified onto the surface of the metal nanoparticles through Ag-S bonds. Subsequently, through a complementary pairing process between the ADNA and cDNA chains, a cDNA chain with a Cy3 terminus at one end is brought close to the metal nanoparticles to synthesize a nucleic acid aptamer sensor. The metal nanoparticle reagent kit can specifically recognize and rapidly capture eczema biomarkers using nucleic acid aptamers. Combined with surface-enhanced Raman spectroscopy, it achieves rapid, sensitive, and accurate qualitative and quantitative detection of eczema biomarkers. Simultaneously, the internally encapsulated internal standard molecule signal can dynamically correct the Raman signal in the metal nanoparticle reagent kit.

According to the cyclization assembly of the linear RNA and the self-cyclization method of the RNA chain, cyclization primitives are connected to the two ends of an RNA sequence to be cyclized, the 3'end and the 5 'end of the RNA sequence are made to be very close in space by means of complementary pairing interaction of bases between the cyclization primitives, and cyclization of target RNA is completed by connecting the 3' end and the 5 'end of the RNA sequence through RNA ligase. Compared with linear RNA, the cyclized RNA prepared by the method has higher stability.

The invention relates to the technical field of lithium ion batteries, in particular to a modified binder, a preparation method thereof and a secondary battery. The modified binder comprises the following raw material components: a carboxyl-containing polymer binder; a nitrogen-containing heterocyclic group is grafted in a molecular chain of the polyurethane, and the nitrogen-containing heterocyclic group contains a hydrogen bond acceptor group and a hydrogen bond donor group; the cross-linking agent contains a hydrogen bond donor group, a hydrogen bond acceptor group and a positive charge group. The carboxyl-containing polymer binder can provide basic bonding performance; and the polyurethane can exert the flexibility and elasticity. On the basis, the polyurethane and the cross-linking agent form a hydrogen bond complementary pairing mode similar to DNA and RNA, so that the cross-linking agent can accurately recognize nitrogen-containing heterocyclic ring groups on the polyurethane, and the bonding strength is high. And the cross-linking agent and the carboxyl-containing polymer binder generate an electrostatic adsorption effect. The modified binder has high flexibility, elasticity and bonding performance, and effectively relieves impact caused by volume expansion of the silicon-based negative electrode material.

Reduced delay and improved threshold tracking over PVT in a comparator input circuit and comparator are provided by circuits that include an inverter stage having a first complementary pair of transistors connected in a push-pull configuration. An input of the inverter stage is coupled to the gates of the first complementary pair of transistors, and an output of the inverter is coupled to the drains of the first complementary pair of transistors. The circuits also include one or more first degeneration transistors coupled between a source of one of the first complementary pair of transistors and a power supply rail of the circuit, and a reference circuit with an output coupled to one or more gates of the first degeneration transistors. The reference circuit controls the one or more first degeneration transistors to cause the inverter stage to have a threshold voltage equal to a threshold control voltage.

The application discloses a nucleic acid multicolor fluorescent probe and application thereof in preparation and in-situ real-time observation of sequential activation of a P53-mediated cell apoptosis pathway. A P53 fixed chain, a Bax fixed chain and a Cyt c fixed chain are modified on the surface of S-AuNSs@SiO2 through an amide bond, and a complementary chain modified with different fluorescent groups, an aptamer chain are connected through a complementary pairing rule to construct a multicolor fluorescent probe. In the presence of a target, DNA double strands are forced to be opened, and a fluorescent group is released from the system to restore quenched fluorescence. Due to 20 symmetrical 'hot spots', a stronger local electric field can be generated, and the probe based on S-AuNSs shows better optical performance. The S-AuNSs@SiO2-P of the application has high integration, is easy to obtain, and realizes in-situ real-time observation of sequential activation of a P53-mediated apoptosis pathway in living cells, and has application prospects in mechanism research, drug discovery, dynamic monitoring of an apoptosis process and the like.

The invention relates to a self-assembly preparation method of a sub-10-nanometer spacing electrode pair, which comprises the following steps: by taking DNA two-dimensional rectangular origami as a DNA template, respectively extending two groups of single-stranded DNA, namely extension chains, on the surface of the DNA template, respectively taking the two groups of extension chains as two connecting sites, respectively modifying gold nanorods by adopting two pairing chains to obtain gold nanorods modified by the two pairing chains, the two pairing chains are sulfydryl modified single-stranded DNA matched with the connecting sites respectively, and accurate directional arrangement of the gold nanorods on a DNA template is achieved by means of the complementary base pairing principle of DNA. Compared with the prior art, the method has the advantages that the distance between the electrode pairs is stabilized at the sub-10 nanometer scale, complicated photoetching equipment is not needed, the process flow is simple, the cost is relatively low, and a technical basis for batch preparation is provided for the development of the sub-10 nanometer electrode pairs of nanometer electronic devices.

Provided is an oligonucleotide capable of inducing the editing activity of ADAR in a cell and having excellent stability. The oligonucleotide includes a first oligonucleotide and a second oligonucleotide linked to the 5' side of the first oligonucleotide, and is capable of inducing the site-specific editing in the target RNA. The first oligonucleotide is composed of a target-corresponding nucleoside residue corresponding to an adenosine residue in the target RNA, an oligonucleotide containing 3 to 6 residues, linked to the 5' side of the target-corresponding nucleoside residue, and having a base sequence complementary to the target RNA and an oligonucleotide containing 10 to 24 residues, linked to the 3' side of the target-corresponding nucleoside residue, and having a base sequence complementary to the target RNA, and at least one phosphorus-containing linking group selected from the group consisting of a phosphorus-containing linking group linking a 1st nucleoside residue and a 2nd nucleoside residue and a phosphorus-containing linking group linking a 4th nucleoside residue and a 5th nucleoside residue, counting in the 3' direction from the target-corresponding nucleoside residue, is at least one selected from the group consisting of an alkylphosphonic residue, an alkyl phosphate residue, and a substituted phosphoramide residue. At the 3' end of the second oligonucleotide, a nucleoside residue corresponding to a nucleoside residue of the target RNA is deleted, or, at the 3' end, the second oligonucleotide has a nucleoside residue that does not form a complementary pair with the nucleoside residue of the target RNA, and contains 2 to 10 residues, and nucleoside residues at least other than at the 3' end are complementary to the target RNA.

An aspect of the present disclosure features a multi-phase converting circuit, including a primary side switch circuit. The primary side switch circuit includes multiple transistor complementary pairs. The multiple transistor complementary pairs are parallel-connected. The multi-phase converting circuit also includes resonant tank coupled to the primary side switch circuit and including multiple capacitors, multiple resonant inductors and multiple transformers. The multi-phase converting circuit also includes a secondary side switch circuit. The secondary side switch circuit is formed by a full bridge structure. Each transistor complementary pair includes a first transistor and a second transistor. The first transistor and the second transistor are serial-connected. Each transistor complementary pair includes a node between the first transistor and the second transistor. The node is coupled to respective one of the resonant inductors.

A wireless communication method and related apparatus are provided in embodiments of the present disclosure. A first node obtains a first / 2 BPSK Golay sequence based on a first Golay sequence. The first / 2 BPSK Golay sequence is a sequence of a first / 2 BPSK Golay complementary pair (GCP), the first Golay sequence is a sequence of a first GCP, and the first / 2 BPSK GCP is a GCP or a quasi GCP. The first node transmits a reference signal based on the first / 2 BPSK Golay sequence. The first / 2 BPSK Golay sequence combines the / 2 BPSK technique and the Golay sequence, hence good auto-correlation property of the first / 2 BPSK Golay sequence is guaranteed, making it possible for the first node to transmit the reference signal with low peak to average power ration (PAPR).