6results about How to "Simplify the experimental steps" patented technology

The application belongs to the technical field of medicine, and particularly relates to a method for embedding micro-organoids or cells with agarose. The method for embedding micro-organoids or cells with agarose comprises the following steps: 1) fixing micro-organoids or cells with a fixing solution; 2) washing the fixed organoids or cells, removing supernatant after centrifugation, and obtaining organoid or cell precipitate; 3) pre-embedding organoids or cells with agarose; 4) taking out the agarose pre-embedded organoids or cells after demolding, and transferring the agarose pre-embedded organoids or cells to an embedding box, and sequentially performing ethanol gradient dehydration, transparent treatment and paraffin wax immersion treatment on the sample according to a specific procedure; and 5) paraffin embedding. Compared with the prior art, the application has the following beneficial effects: improving organoid / cell density; by agarose embedding, reducing the relative dispersion volume of organoids / cells, significantly improving the density of the sample in the slice, facilitating observation and detection, and having good detection effect.

This application discloses an in-situ permeability testing system for underground coal gasification, including a core holder comprising a cylinder, a seepage ring, an axial pressure loading component, a gas pipeline, a sealing assembly, a confining pressure sleeve, a core heating element, a temperature and flow rate measurement assembly, an inlet gas pressure measurement assembly, an outlet gas pressure measurement assembly, and a permeability calculation assembly. The system can calculate the permeability of the core using the core holder outlet temperature, outlet flow rate, inlet gas pressure, and outlet gas pressure, combined with the core's dimensional parameters and the gas viscosity at the core holder outlet temperature. This system can simulate the high-temperature, high-pressure, and high-stress environment of deep underground coal gasification and directly perform in-situ core permeability testing, better ensuring the accuracy and reliability of the test results. This application also discloses an in-situ permeability testing method for underground coal gasification.

This invention discloses a method for predicting gate switch oscillation in GaN-HEMT devices, belonging to the field of semiconductors and power devices. The invention trains a BPNN network model to obtain a preliminary mapping relationship between parasitic capacitance and gate-source voltage based on the BPNN algorithm model. Then, a Cascode-based two-port network function for GaN HEMT is established. The parasitic capacitance value of the GaN HEMT device under all S-parameters is obtained experimentally. This extracted parasitic capacitance value is then input into the BPNN network model as a dataset to obtain Vo. GS_m V under all S parameters DS C GS C GD The relationship between the surface and the curve is shown. Simulation results show that the standard error of the prediction method of the present invention is smaller than that of ANN and PSO, proving that the detection method for GaN-HEMT gate switch oscillation based on the BPNN algorithm model of the present invention has high prediction accuracy.

This invention provides a siloxane ligand-protected Ag 40 Nanoclusters, their synthesis methods, and applications. This invention relates to Ag protected by siloxane ligands. 40 Nanoclusters are composed of RC≡C ‑ and (Ph 4 Si 4 O 8 ) 4‑ Ag protected by both ligands 40 Nanoclusters, with the molecular formula {Ag} 40 [(RC≡C) 8 (Ph 4 Si 4 O 8 ) 6 The silver nanoclusters synthesized in this invention have precise atoms and novel structures; the synthesis method is simple, easy to operate, mild under mild conditions, and the raw materials are readily available, making it potential for industrial and commercial production; the obtained silver nanoclusters exhibit superior fluorescence performance at low temperatures and have a long fluorescence lifetime, making them practically valuable in the field of fluorescent probes and worthy of market promotion.

The application discloses a kind of experimental device and experimental method of the effect of gas-producing bacteria on plant growth, including experiment cabin and control system, experiment cabin is divided into upper plant culture area and lower microbial culture area by horizontally arranged partition, planting substrate is placed at the top of partition, a plurality of air holes are provided on the partition, environmental simulation control system is provided in plant culture area, sterilization system and environmental control system are provided in microbial culture area, environmental simulation control system, sterilization system, environmental control system are electrically connected with control system.The technical scheme of the application integrates microbial culture part and plant planting part, gas produced by gas-producing bacteria can directly act on plants, without the need for intermediate collection link, which simplifies the experimental steps, avoids errors generated during transfer, and improves the accuracy of experimental research.

This invention belongs to the field of biotechnology applications and discloses a gene containing a kinesin domain that is associated with the height trait of the first fruiting branch of cotton. GhFFBPH And its applications. This invention provides the cDNA sequence (SEQ ID NO.1) and genome sequence (SEQ ID NO.2) of this gene in tetraploid upland cotton TM-1, and the gene... GhFFBPH The gene contains a non-synonymous SNP located at 353 bp in the coding region, where the base changes from A to T, and the corresponding amino acid changes from Gln to Leu. The gene provided in this invention was obtained from cotton variety resequencing and genome-wide association analysis, and is significantly associated with the height trait of the first fruiting branch in cotton. Two haplotypes of this gene are used to distinguish the height phenotype of the first fruiting branch: the AA haplotype corresponds to the tall phenotype, and the TT haplotype corresponds to the short phenotype. This invention... GhFFBPH Genes have significant research value and application prospects in improving the height of the first fruiting branch of cotton and in breeding new varieties of cotton with high first fruiting branch height.