558results about How to "Simplify the experimental steps" patented technology

The invention relates to an integrated micro-fluidic chip for screening drug compatibility, which comprises a fluid flow passage layer, a microvalve control channel layer and a glass substrate layer, wherein the fluid flow passage layer and the microvalve control channel layer are thermally bonded through PDMS to form a fluid flow passage unit, the fluid flow passage unit and the glass substrate layer form a whole through plasma bonding, the fluid flow passage unit comprises two functional areas, namely, a medicine mixing generation area and an array cell culture area. The invention further provides a preparation method and an application of the integrated micro-fluidic chip for screening drug compatibility; an SU-8 negative photoresist technology, an AZ-XT50 positive photoresist technology, a multi-layer PDMS thermal bonding technology and a plasma bonding technology are adopted, and the micro-fluidic chip integrating functions of automatic medicine mixing and generating, cell culture, excitation, parallel analysis on compatibility results of different drugs, real-time observation and result detection is prepared, multiple experiment parameters can be obtained through once operation, and the integrated micro-fluidic chip is applied to the research on the high-flux medicine compatibility relationship.

The invention provides a micro fluidic chip for sorting and whole genome amplification of a single cell. The micro fluidic chip comprises a four-layer structure, wherein the four layers are stacked together in order and mutually sealed, and the structure comprises the following layers from top to bottom: a top cover, a micro valve control layer, a valve plate layer and a channel layer; and the bottom of the micro fluidic chip is externally provided with a magnet. The invention also provides a system for screening and identifying cells as well as amplification and analysis of a single cell, and the system is matched with the chip. The micro fluidic chip and the corresponding detection system can be used for sorting target cells from a large amount of cells quickly, simply and cheaply, and amplifying and analyzing whole genome DNA of a single cell therein.

The invention provides a method for preparing a multi-sample deoxyribonucleic acid (DNA) mixed sequencing library, which comprises the following steps of: converting a DNA chain fragment of a sample into a DNA fragment which is hundreds of basic groups long; connecting upper Y-shaped joints at both ends of the DNA fragment; performing polymerase chain reaction (PCR) amplification of the DNA fragment connected with the upper Y-shaped joints; and mixing DNAs of various samples according to the arrangement of a computer sequencing channel and the difference between label sequences, wherein the Y-shaped joints comprise label sequences for distinguishing different samples; and a first primer for the PCR amplification and a second primer for the PCR amplification, besides sequences which are complementary to the Y-shaped joints, also comprise sequences which are complementary to two surface primers in a sequencing platform of the second generation respectively, so that the DNA fragments obtained by the amplification can hybridize with the surface primers complementarily in the sequencing process to start the process of sequencing while synthesizing.

The invention discloses a hexagonal boron nitride two-dimensional ultrathin nanometer sheet as well as a preparation method and application thereof and belongs to the technical field of nanometer materials. According to the invention, metal boride (such as calcium boride, lanthanum boride, magnesium boride, titanium boride and the like) is adopted as a boron resource; ammonium salt (such as ammonium chloride, ammonium bromide, ammonium nitrate and the like) is adopted as a nitrogen source; and the hexagonal boron nitride two-dimensional ultrathin nanometer sheet with the thickness of 0.5-4.0nm is obtained through the reaction under a mild condition (at the temperature of 500-600 DEG C). The invention aims to realize the macro-quantity preparation of the hexagonal boron nitride two-dimensional ultrathin nanometer sheet at the mild temperature by adopting cheaper raw materials; the preparation method has the advantages of saving the energy, simplifying the experimental step and greatly reducing the product cost; and due to the high heat conductivity, heat stability and chemical stability, the hexagonal boron nitride two-dimensional ultrathin nanometer sheet can be applied to the fields of heat dissipation materials, polymer filling materials, catalyst carriers and the like.

The invention relates to an analytical method for researching protein structure or protein-protein interaction. The method comprises the following steps: performing crosslinking and enzymolysis on a protein composite in a cell by using a crosslinking agent with reactive groups on two sides and a breakable group, and taking a part of the enzymatic hydrolysate for a derivatization reaction for mass spectrometry; after breaking the crosslinking agent by means of a chemical method for the other part of the enzymatic hydrolysate, enriching peptide sections with an enriching material, and performing mass spectrometry on the enzymatic hydrolysate without the enriched peptide sections; determining the crosslinked peptide sections according to a library searching result so as to establish a peptide section library; finding out a candidate peptide section from the peptide section library according to N-terminal amino acid information of the crosslinked peptide section determined in the mass spectrogram of the crosslinked peptide section; and determining the crosslinked peptide section sequence by combining the mass spectrogram m/z of the crosslinked peptide section and the characteristic ions of the peptide section so as to obtain the protein structure and protein-protein interaction information. The method has the advantage of being simple to operate, and is applied to structural analysis of proteins and analysis of protein-protein composite interaction.

The invention relates to the field of molecular biology and provides Oligo dT primer, wherein Oligo dT primer is a single-stranded DNA molecule containing continuous dT sequences and recognition sites for cutting. The invention further provides a method for constructing a cDNA library on the basis of the Oligo dT primer. The Oligo dT primer is excellent in applicability, can be applied to various different library schemes, and can further accurately locate the initiation site of mRNA molecule in poly A tail. The method for constructing the cDNA library can ensure that all mRNA molecules in a sample can show specificity in the constructed cDNA library.

The invention relates to a method for preparing mesoporous gamma-Al2O3 by a dynamic hydrothermal method, belonging to the field of preparation of inorganic porous materials. The invention aims to solve the problems that in the prior art, reaction steps are multiple, experimental conditions are difficult to control, products are incompletely crystallized, the reaction time is too long, and a static reaction does not facilitate full contact among reaction materials. The method comprises the following steps of: adding inorganic aluminum salt and a precipitating agent into surfactant solution respectively, and uniformly mixing the inorganic aluminum salt with the precipitating agent; continuously stirring the mixed solution, and performing hydrothermal treatment; and washing and calcinating obtained products after finishing the reaction, and thus obtaining the mesoporous gamma-Al2O3. The method has the advantages that: the reaction is performed more fully, the formation of alumina precursors can be simply controlled, the formation of mesoporous alumina can be effectively controlled, the crystallization time is shortened to be less than 24 hours, the preparation period is short, and the method is suitable for large-scale continuous production.

The invention provides a preparation method of a catalytic material mixed by copper and cerium oxide. The preparation method comprises the following steps of: (a) dissolving copper salt in a solvent, and continuously mixing the copper salt for 0.5-1 hour, wherein the concentration of Cu ions is 0.00872-0.17492 mol/mL; (b) adding mesoporous cerium oxide powder into a copper salt solution obtained by the step (a) according to the liquid-to-solid ratio of 10:1 to 6:1, stilling the copper salt solution for 24-48 hours after the mesoporous cerium oxide powder is dissolved, and meanwhile, aperiodically stirring the copper salt solution; (c) drying the mixed solution obtained by the step (b) at 110-120 DEG C for 10-12 hours, grinding the dried resultant, and calcining the resultant at 200-300 DEG C for 2-3 hours, thus obtaining the catalytic material mixed by the copper and the cerium oxide. The preparation method has the advantages of simplicity in operation and experimental procedure, low cost of raw materials, and no environmental pollution, and the prepared catalytic material has the advantages of large specific surface area and pore volume, high low-temperature activity and favorable thermal stability. The prepared catalytic material can be widely applied to optical CO oxidation sensors, motor vehicle exhaust gas cleaning and purification systems and the like, and is prone to industrial production.

The invention relates to an in-situ infrared spectrum cell which comprises a closed cell body, wherein an infrared injecting port and an infrared ejecting port are oppositely formed in the cell body and are used for enabling infrared light to be transmitted in and penetrate through the cell body; a lighting port and a sample setting port are oppositely formed in the cell body further; the lighting port is used for enabling the light to be transmitted into the cell body and irradiated to a sample to be detected; a packaging structural body which is used for sealing the sample setting port is further arranged at the sample setting port. The packaging structural body further comprises a heating element and a temperature measuring element, wherein the heating element is used for directly heating the sample to be detected, and the temperature measuring element is used for collecting the temperature of the sample to be detected.

The invention relates to a high-temperature and high-pressure visual seepage experimental device and method for real sandstone. The device comprises a real sandstone high-temperature and high-pressuremicroscopic visual model arranged in a high-temperature and high-pressure kettle; the high-temperature and high-pressure kettle is connected with a pressure system for pressurizing the real sandstonehigh-temperature and high-pressure microscopic visual model; the high-temperature and high-pressure kettle is provided with a microscope; the real sandstone high-temperature and high-pressure microscopic visual model comprises a rock sheet; the lower surface of the rock sheet is provided with first glass; the first glass and the rock sheet are connected through an adhesive; two holes are formed in the first glass; a distance between a model fluid inlet and a model fluid outlet is greater than the length of the rock sheet; the upper surface of the rock sheet is provided with second glass; andthe side walls of the rock sheet are respectively provided with a box dam with an inlet guide groove and a box dam with an outlet guide groove along the width direction of the rock sheet. Because therock sheet is a real reservoir sample, the rock sheet has real reaction performance, and a corresponding visual seepage experiment can be carried out under high temperature and high pressure. The device has the advantages of simple structure, flexible operation and high efficiency.

The invention discloses a flame-retardant additive, a preparation method therefor and an application of the flame-retardant additive. In view of that phosphorus-containing flame retardants in the prior art have many performance disadvantages and the problem of high manufacturing and service costs, the invention provides a novel phosphorus-containing flame retardant. The flame retardant is used asa flame-retardant additive in an electrolyte of a non-aqueous electrolytte secondary battery. The invention simultaneously provides the preparation method for the flame retardant. The phosphorus-containing flame retardant prepared by the method not only can effectively improve safety of batteries, but also has a specific positive-negative pole membrane forming function, and thus, comprehensive properties such as high-/low-temperature, cycling and storage properties of the secondary battery can be remarkably improved. The preparation method for the flame retardant, provided by the invention, has the advantages that the raw material cost is low, the preparation process is simple in step, the operation is safe, the product purity is high, and the environmental pollution is light.

The invention provides a novel chitosan carbanilate-carbamido derivative synthetic method, the method employs chitosan and phenyl isocyanate with different groups to react, and then the hydroxy and amino on chitosan can be completely conversed to the chitosan carbanilate-carbamido derivative of carbamate and carbamido. According to the invention, a coating process is employed to prepare the derivative to a chiral stationary phase, and high performance liquid chromatography is used for resolution of various enantiomers, and the chiral stationary phase has high chiral identification capability.

The invention provides a micro-fluidic chip based on a magnetic fluorescence composite particle. The micro-fluidic chip comprises a chip bottom-layer plate, a chip channel layer and a chip upper-layer cover plate which are sequentially arranged from bottom to top, wherein the chip channel layer comprises a sample introduction area, a sample introduction channel, an optical detection area, a waste liquid channel and a waste liquid storage area which are sequentially connected; a capture recognition reagent is fixed to the lower side of the optical detection area; mark recognition reagent-magnetic fluorescence composite particles are pre-fixed on the inner upper sides of the sample introduction area, the sample introduction channel or the optical detection area. The invention also provides an optical detection device. The micro-fluidic chip provided by the invention can be used for sample trace substance analysis through magnetism enrichment and separation reaction and a fluorescence optical detection technology, meets the requirements on quick, sensitive and convenient detection of low-concentration substances in a micro-sample, solves the problems of complicated and expensive instrument equipment, inconvenience, slow detection speed and the like of an existing method, and has a favorable application value in the POCT (Point-of-Care Testing) detection field.

The invention discloses a hydrothermal method for preparing triclinic-phase FeVO4 micro particles. The method comprises the following steps of: mixing ferric nitrate nonahydrate, ammonium metavanadate and nitric acid solution in a molar ratio of 1:1:10, adjusting the pH value to 4 or 7 by using 14 weight percent of aqueous ammonia, transferring the solution to a stainless steel self-pressure kettle of which a lining is made of polytetrafluoroethylene, treating the solution for 2 or 6 hours at the constant temperature of 180 DEG C, filtering the solution, washing and drying the filtrate, and heating the filtrate to 600 DEG C from room temperature at the speed of 1 DEG C per minute under air atmosphere and keeping the temperature for 6 hours, wherein when the pH is 4 or 7 and the hydrothermal treatment is performed for 6 hours, the obtained product is a triclinic-phase FeVO4 mesoporous micro bar, and when the pH is 4 and the hydrothermal treatment is performed for 2 hours, the obtained product is a triclinic-phase FeVO4 imperforate micro block. The method is convenient to operate, the raw materials are cheap, and the topography, crystalline phase structure and specific surface area of the target product particles are controllable.

The invention provides a method for measuring organic matter in soil, which uses a sand bath instead of an oil bath for heating, so that the measurement process is safer; experimental steps are simplified, work efficiency is improved, and experimental consumption costs are reduced. The method for measuring organic matter in soil provided by the invention has a relative standard deviation of 2.86%, and better data reliability and accuracy; the relative standard deviation of the existing method is 3.56%. The invention provides a method for measuring organic matter in soil, and the measuring accuracy is improved by 19.94%.

The invention relates to preparation processes of bambuterol hydrochloride and an intermediate thereof. The bambuterol hydrochloride is synthesized by taking 3,5-dihydroxyacetophenone as a starting raw material and undergoing esterifying, brominating, reducing, condensing and salting reactions. Compared with other processes, the process has the advantages that: the technical problems of industrial production are solved, each step of reaction is simple, and conditions are mild; and the process is a preparation process with the lowest cost at present, and is successfully applied to industrial production.

The invention discloses a method for analyzing propolis chemical components through pyrolysis gas chromatography-mass spectrometry. The method comprises the steps that: propolis powder is placed in a pyrolysis device; the pyrolysis device is placed at a feeding port of a gas chromatograph; when the temperature of the pyrolysis device reaches 400-600 DEG C, feeding is carried out; with a method in which a linear temperature increasing retention index is combined with a mass spectrometry standard spectral library, the chemical components of the propolis are qualified; and the propolis chemical components are subjected to quantitative analysis through calculating areas of the peaks in the gas chromatography spectrum. Solid sample is directly fed into pyrolysis gas chromatography, and no solvent is adopted, such that operation is simple and environment-friendly. The pyrolysis gas chromatography-mass spectrometry method is developed for carrying out qualitative and quantitative detections upon the pyrolysis sample, such that experiment time can be effectively saved, experiment steps can be simplified, and propolis quality can be rapidly evaluated. The detection technology can be popularized to a pyrolysis quality detection field, and has an important significance upon rapid evaluation of pyrolysis quality.

The invention relates to a coding sequence of anti-Cry1Ac scFv and an immuno-PCR detection method for detecting Cry1Ac through the phage scFv. The scFv are obtained through elutriation in a natural phage antibody base according to the method. The method is characterized in that the specificity of the scFv is determined by a CDR region sequence in a heavy chain and light chain variable region sequence, immuno-PCR results indicate that a fluorescence detection system can be used for quantitative detection of the Cry1Ac, and the detection range is 0.2-100ng/mL. The method is fast, simple and high in flexibility; a step for marking reporter DNA molecules during an existing immuno-PCR process is avoided, the experimental operation is simplified, and potential practical values are provided.

The invention discloses a medlar sterile seedling and a method for obtaining the induced healing of the medlar sterile seedling, belonging to the technical field of plant tissue culture. The method comprises the following steps of drying fruits; disinfecting seeds: carrying out the seed disinfection by using sterile distilled water which contains 70 percent by weight of ethanol and 0.1 percent by weight of TritonX-100 (polyethylene glycol octylphenol ether); germinating seeds: firstly arranging the seeds into a refrigerator at 4 DEG C, standing for 2 days, and then removing the seeds to a culture room at the temperature of 25+/-2 DEG C with the daily photoperiod of 14 hours to culture for 20+/-2 days; healing subculture: arranging the seed on a subculture medium, culturing under at 25+/-2 DEG C in the culture room with the daily photoperiod of 16 hours, and replacing the culture medium every two weeks. According to the method disclosed by the invention, both the medlar sterile seedling and the medlar induced callus are obtained, the step of carrying out physical trauma on the medlar callus in the inducing process of the medlar callus is simplified and the period of obtaining the medlar callus is shortened.

The invention aims to provide a method for measuring transmission loss of an asymmetric pipeline silencer. The method comprises the following steps: a microphone is arranged in one fan-shaped area in an inlet and outlet pipeline, and incident sound pressure and transmission sound pressure of the area are measured; and the prior step is repeated according to the number of partition boards to respectively measure the incident sound pressure and the transmission sound pressure in each fan-shaped area in the inlet and outlet pipeline, and the transmission loss is computed according to the formula disclosed in the specification. According to the method disclosed by the invention, the precision within an inlet and outlet plane wave cut-off frequency range of the conventional measurement method can be met, the silencing performance of the silencer after the frequency is higher than the inlet and outlet plane wave cut-off frequency can be precisely detected, and the problem that the acoustic performance of a large-caliber asymmetric silencer at high frequency can not be accurately forecast is solved.