18results about How to "Simplify the experimental steps" patented technology

The invention provides a stretchable in-situ soaking double-network hydrogel electrolyte as well as a preparation method and application thereof. The method comprises the following steps: preparing a uniformly mixed solution of a first monomer, a second monomer, an initiator, a cross-linking agent and an accelerator; adjusting the pH value of the mixed solution, and uniformly stirring to obtain a precursor solution; removing bubbles in the precursor solution, and carrying out free radical polymerization at a preset temperature to form hydrogel; and cutting the hydrogel, dropwise adding a trace amount of a salt solution containing zinc ions to two sides of the cut hydrogel, and clamping the hydrogel between two pole pieces for in-situ soaking to obtain the hydrogel electrolyte. By using the independent hydrogel electrolyte, side reaction on the surface of the zinc negative electrode, growth of dendritic crystals and electrode corrosion can be inhibited, and meanwhile, the electrochemical performance of the zinc ion battery is improved.

The application belongs to the technical field of medicine, and particularly relates to a method for embedding micro-organoids or cells with agarose. The method for embedding micro-organoids or cells with agarose comprises the following steps: 1) fixing micro-organoids or cells with a fixing solution; 2) washing the fixed organoids or cells, removing supernatant after centrifugation, and obtaining organoid or cell precipitate; 3) pre-embedding organoids or cells with agarose; 4) taking out the agarose pre-embedded organoids or cells after demolding, and transferring the agarose pre-embedded organoids or cells to an embedding box, and sequentially performing ethanol gradient dehydration, transparent treatment and paraffin wax immersion treatment on the sample according to a specific procedure; and 5) paraffin embedding. Compared with the prior art, the application has the following beneficial effects: improving organoid / cell density; by agarose embedding, reducing the relative dispersion volume of organoids / cells, significantly improving the density of the sample in the slice, facilitating observation and detection, and having good detection effect.

This application discloses an in-situ permeability testing system for underground coal gasification, including a core holder comprising a cylinder, a seepage ring, an axial pressure loading component, a gas pipeline, a sealing assembly, a confining pressure sleeve, a core heating element, a temperature and flow rate measurement assembly, an inlet gas pressure measurement assembly, an outlet gas pressure measurement assembly, and a permeability calculation assembly. The system can calculate the permeability of the core using the core holder outlet temperature, outlet flow rate, inlet gas pressure, and outlet gas pressure, combined with the core's dimensional parameters and the gas viscosity at the core holder outlet temperature. This system can simulate the high-temperature, high-pressure, and high-stress environment of deep underground coal gasification and directly perform in-situ core permeability testing, better ensuring the accuracy and reliability of the test results. This application also discloses an in-situ permeability testing method for underground coal gasification.

The utility model relates to a whole membrane multi-antibody incubation box used for Western Blot. A box cover is rotatably arranged on a box body, a plurality of partition plates are arranged in the box body, the inner space of the box body is divided into a plurality of cells through the partition plates, the left ends and the right ends of the partition plates are lightly pressed on the two sides of the upper surface of the box body, and the partition plates can slide on the upper surface of the box body. Scale marks are arranged at the bottom of the box body, so that the partition plates can partition neatly. Liquid-leakage-proof sealing silica gel strips are arranged on the upper surface of the box body and the periphery of the partition plate, so that the sealing performance of each cell is ensured, and antibody leakage is avoided; a cover-in-cover is rotatably arranged in the middle of the box cover. The partition plate is limited through the box cover and the sealing silica gel strip, so that the partition plate does not incline laterally; the cover-in-cover is arranged, so that the antibody is not interfered by external factors, and the incubation condition of the antibody can be clearly observed. Operation is easy and convenient, the application range is wide, exquisite and convenient, and cost can be saved.

This invention discloses a method for predicting gate switch oscillation in GaN-HEMT devices, belonging to the field of semiconductors and power devices. The invention trains a BPNN network model to obtain a preliminary mapping relationship between parasitic capacitance and gate-source voltage based on the BPNN algorithm model. Then, a Cascode-based two-port network function for GaN HEMT is established. The parasitic capacitance value of the GaN HEMT device under all S-parameters is obtained experimentally. This extracted parasitic capacitance value is then input into the BPNN network model as a dataset to obtain Vo. GS_m V under all S parameters DS C GS C GD The relationship between the surface and the curve is shown. Simulation results show that the standard error of the prediction method of the present invention is smaller than that of ANN and PSO, proving that the detection method for GaN-HEMT gate switch oscillation based on the BPNN algorithm model of the present invention has high prediction accuracy.

The invention discloses a method for reducing gold and platinum noble metal nanoparticles through silver nanowires, which is characterized in that gold and platinum noble metal nanoparticles are replaced on the silver nanowires through simple replacement reaction, and the original micromorphology of the silver nanowires is maintained. By controlling the amount, the adding mode and the adding condition of the added noble metal acid, selective replacement of the silver nanowires can be achieved, and the growth size and distribution of the nanoparticles can be controlled. The prepared AgNWs-Au and AgNWs-Pt with the root nodule-like structures are uniform in form and controllable in growth, the preparation process is simple and rapid, the reproducibility is high, and the obtained sample is stable in property. The development requirement of a material subject on a multifunctional composite material is met to a certain extent, and the composite material has a wide application prospect in the fields of electro-catalysis and sensing.

The utility model discloses continuous equipment for preparing an expanded graphite material from a negative electrode of a waste lithium ion battery. The equipment comprises a pickling device, a strong oxidation impurity removal device and a centrifugal device which are sequentially communicated through a pipeline, the grabbing device comprises a track and a mechanical claw which is arranged on the track and can move along the track and move up and down, the track is arranged above the centrifugal device, the drying device and the high-temperature expansion device which are sequentially arranged, and the mechanical claw is used for conveying device materials below the track. The waste graphite negative electrode is safely converted into graphite oxide through the pickling device, the strong oxidation impurity removal device and the centrifugal device, then the graphite oxide is sequentially moved to the drying device from the centrifugal device through the grabbing device, and finally the graphite oxide is converted into high-value porous expanded graphite in the high-temperature expansion device. High-value utilization of low-carbon, efficient, green and sustainable retired lithium ion battery negative electrodes is achieved, and the method is suitable for continuous large-scale production of expanded graphite materials.

The present invention relates to the field of molecular biology detection technology, and in particular to a primer combination, a kit and a method for detecting human neutrophil antigen genotyping. The present invention designs a forward primer, a reverse primer and an extension primer for all 11 SNP sites of the human granulocyte antigen HNA 1-5 system, and the sequence is shown in SEQ ID No: 1-22. The method for detecting human neutrophil antigen genotyping using the primer combination can achieve batch detection of 96 or 384 samples per batch, and high-throughput detection will bring great convenience to related clinical diagnosis and scientific research work, greatly save experimental time, and the detection method is accurate and stable.

The invention discloses a travertine pore determination method and system based on a polarizing microscope and a scanning electron microscope. The method comprises the following steps: collecting and preparing a travertine sample, and dividing lithofacies types of the travertine sample; determining the micro-pore distribution position of the travertine sample through a polarizing microscope; microscopic pore characteristics of the travertine sample are observed through a scanning electron microscope; and determining the components of the travertine sample through an energy disperse spectroscopy, and dividing pore types. According to the method, the time consumption for finding the travertine micro-pores is effectively reduced, the pore type division efficiency is improved, the experiment efficiency is improved, and the observation time is saved.

This invention provides a siloxane ligand-protected Ag 40 Nanoclusters, their synthesis methods, and applications. This invention relates to Ag protected by siloxane ligands. 40 Nanoclusters are composed of RC≡C ‑ and (Ph 4 Si 4 O 8 ) 4‑ Ag protected by both ligands 40 Nanoclusters, with the molecular formula {Ag} 40 [(RC≡C) 8 (Ph 4 Si 4 O 8 ) 6 The silver nanoclusters synthesized in this invention have precise atoms and novel structures; the synthesis method is simple, easy to operate, mild under mild conditions, and the raw materials are readily available, making it potential for industrial and commercial production; the obtained silver nanoclusters exhibit superior fluorescence performance at low temperatures and have a long fluorescence lifetime, making them practically valuable in the field of fluorescent probes and worthy of market promotion.

The present invention discloses an electrochemiluminescent immunosensor of TiO2 nanoflowers loaded with CdTe quantum dots and its preparation method and application. The CdTe / TiO2 NFs composite material is prepared and used as an electrochemiluminescent signal marker. The surface and interior of TiO2NFs can load a large amount of CdTe QDs, and at the same time can catalyze the CdTe QDs / S2O8 2‑ The co-reaction process of the system significantly improves the electrochemiluminescence intensity of CdTe / TiO2 NFs. The magnetic material Fe3O4@SiO2 NPs is used as a magnetic separation carrier, which has the advantages of superparamagnetism, good biocompatibility, and easy biomodification. The present invention constructs an electrochemiluminescence immunosensor based on the interaction between antigen and antibody. The sensor has the characteristics of high sensitivity, strong specificity, good stability, low detection limit, wide linear range, and simple operation.

The utility model relates to the technical field of experiment detection centrifugal tubes, in particular to a sleeve type centrifugal tube which comprises an outer tube, an inner hose is arranged in the outer tube, and the outer tube and the inner hose are connected with each other through a fixing assembly; the inner hose comprises a hose body and a conical part, the hose body and the conical part are connected through a connecting assembly, and the connecting assembly is used for connecting the hose body and the conical part in a sealed mode. When the device is used, through the outer tube and the inner hose which are arranged in a nested mode, the inner hose can be directly taken out by clamping a hanging lug through tweezers after centrifugation is conducted, then a specimen in a conical part can be rapidly taken out after the conical part is taken down by damaging a connecting assembly, and the experiment steps are greatly simplified; compared with an existing centrifugal tube, the caliber of the bottom of the conical part is smaller, so that cell culture with a small cell quantity can be conveniently collected and obtained, the cell culture can be stored for a long time, and the quantity of cultured organoids can be reserved to the maximum extent.

The application discloses a kind of experimental device and experimental method of the effect of gas-producing bacteria on plant growth, including experiment cabin and control system, experiment cabin is divided into upper plant culture area and lower microbial culture area by horizontally arranged partition, planting substrate is placed at the top of partition, a plurality of air holes are provided on the partition, environmental simulation control system is provided in plant culture area, sterilization system and environmental control system are provided in microbial culture area, environmental simulation control system, sterilization system, environmental control system are electrically connected with control system.The technical scheme of the application integrates microbial culture part and plant planting part, gas produced by gas-producing bacteria can directly act on plants, without the need for intermediate collection link, which simplifies the experimental steps, avoids errors generated during transfer, and improves the accuracy of experimental research.

The invention relates to a double-promoter expression vector and a protein expression detection method thereof, and belongs to the technical field of molecular biology and cell engineering. On the basis of an eukaryotic expression vector, a prokaryotic cell recognized T5 promoter, a ribosome binding site (RBS) and a Kozak sequence GCCACC are added, so that the same vector can express target protein in two different types of cells. In addition, the invention also provides a matched protein expression detection method, the prokaryotic expression product is quickly verified by using the protein detection card, so that the expression condition of the target protein in mammalian cells is predicted, and the experiment cost and time consumption are obviously reduced. By simplifying the production process of the recombinant protein, the experimental efficiency is effectively improved, and a more convenient and efficient protein expression and detection solution can be provided for scientific researchers and biological medicine enterprises.

The invention relates to a microwave technology-based detection device and method for metallization transformation of a substance under high voltage, the detection device is composed of a microwave generator, a DAC detection unit and a parameter detection device, and the DAC detection unit is connected with the microwave generator and the parameter detection device through microwave transmission lines to realize signal transmission. The DAC detection unit is mainly composed of a microstrip line set, an upper diamond and a lower diamond, and the position with the optimal experiment effect is found by changing the width of the lower microstrip line in the DAC detection unit to measure an S parameter response simulation curve. The mode of combining the microstrip antenna and the diamond anvil cell is adopted to detect the metallization transformation process of the substance under high pressure, the detection device is simple and reliable in structure, low in power consumption and convenient and easy to use, the detection method is low in experiment cost, and experiment steps are simple and easy to operate; the gasket and the to-be-tested sample are convenient to replace, and the device can be used for researching the high-pressure scientific material metallization or material non-gold transformation process.

The invention provides a kit for integrally detecting aging cells and apoptotic cells as well as a detection method and application of the kit. The kit comprises an aging inducer, an apoptosis inducer, an integrated buffer solution, a beta-galactosidase specific fluorescent substrate and an apoptotic cell fluorescent staining solution. The kit disclosed by the invention contains an integrated buffer solution capable of fully meeting the detection requirements of senescence and apoptosis cell populations and transition cell populations, so that the experimental steps can be simplified to the greatest extent, and the experimental cost is saved. Results provided by the kit are visual, data are accurate, the detection accuracy and sensitivity are remarkably improved, and a powerful tool is provided for integrally analyzing and detecting dynamic evolution of the aging process and the apoptosis process through a streaming technology.

This invention belongs to the field of biotechnology applications and discloses a gene containing a kinesin domain that is associated with the height trait of the first fruiting branch of cotton. GhFFBPH And its applications. This invention provides the cDNA sequence (SEQ ID NO.1) and genome sequence (SEQ ID NO.2) of this gene in tetraploid upland cotton TM-1, and the gene... GhFFBPH The gene contains a non-synonymous SNP located at 353 bp in the coding region, where the base changes from A to T, and the corresponding amino acid changes from Gln to Leu. The gene provided in this invention was obtained from cotton variety resequencing and genome-wide association analysis, and is significantly associated with the height trait of the first fruiting branch in cotton. Two haplotypes of this gene are used to distinguish the height phenotype of the first fruiting branch: the AA haplotype corresponds to the tall phenotype, and the TT haplotype corresponds to the short phenotype. This invention... GhFFBPH Genes have significant research value and application prospects in improving the height of the first fruiting branch of cotton and in breeding new varieties of cotton with high first fruiting branch height.

The invention discloses a novel lactose intolerance determination kit and application thereof.According to the kit, urine is adopted for sample detection, the steps are simple and convenient, the detection efficiency is high, the kit can be used for large-scale screening of people, and it is proved through contrast experiments that the self-developed kit is excellent in detection performance and high in sensitivity. And the method has a wide application prospect in screening of people with lactose intolerance.