69 results about "Bead array" patented technology

Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

This invention provides high unit density arrays of microparticles and methods of assembling such arrays. The microparticles in the arrays may be functionalized with chemical or biological entities specific to a given target analyte. The high unit density arrays of this invention are formed on chips which may be combined to form multichip arrays according to the methods described herein. The chips and / or multichip arrays of this invention are useful for chemical and biological assays.

The invention provides a method belonging to the field of cytometric bead array with high sensitivity for testing platelet specific antibody based on platelet basic experimental study. Platelet lysate is used to incubate the microsphere which is coated with platelet membrane glycoprotein monoclonal antibody, then goat-anti-human IgG polyclonal antibody labeled with phycoerythrin is added, which is analyzed in cytometric bead array. If autoantibodies exist on the surface of platelet, 'microsphere-platelet membrane glycoprotein monoclonal antibody-platelet membrane glycoprotein autoantibodies-goat-anti-human IgG polyclonal antibody labeled with phycoerythrin' complex structure is formed, the fluorescence intensity of the test microsphere is enhanced. The invention is easy to operate, and has mature technology, high sensitivity for testing platelet autoantibodies, which is good for basic experimental study of platelet antibody.

Methods of using nuclear magnetic resonance (NMR) to detect the interaction of target substances with probes present at locations of a microarray are disclosed, and in particular methods of detecting the interaction of target substances present in fluids with an array comprising one or more probes present or locatable, e.g. in the case of a bead array, on a substrate at one or more locations. The methods are based on detecting the changes in the NMR signal arising from spin-carrying molecules present in a fluid in the vicinity of the probes that occur when target substances interact with probes in the array.

A large-power LED street lamp power supply circuit system is characterized in that the large-power LED street lamp power supply circuit system comprises an LED power supply controller unit, an LED power supply unit, an LED street lamp bead array unit, a wireless communication module and a temperature sensor. An adjusting method is an intelligent control method based on an expert rule algorithm of an LED life mechanism model, and an LED array temperature prediction model unit and an expert rule unit of the LED life mechanism model. The system and the method have the advantages that reasonable illumination is realized, electric energy is saved, safe working of LED street lamps is ensured, and the failure of the LED and the relatively short service lifetime, which are caused by overheating, are prevented.

The invention relates to a high-power LED road lamp including a metallic heat radiation base panel, a reflecting, a LED lamp bead array and LED electric power drive circuit; the heat radiation base panel has a arch column casing, the LED lamp bead array is arranged on circular arc surface of the arch column casing; the reflecting panel has a reflecting section matching with arch column casing circular arc surface of the heat radiation base panel; the reflecting section mainly comprises panel bodies array with quadrangular frustum casing shape, and lamp bead holes are arranged at center of each panel body with quadrangular frustum casing shape; each LED lamp bead of the LED lamp bead array is in the lamp bead hole with quadrangular frustum casing shape corresponding with the reflecting section of the reflecting panel. In use, according to the invention, light reflected by the reflecting section of the reflecting panel forms a rectangular light beam to project on road, which achieves effect of uniformly irradiating reflection ray on road; the whole configuration of the reflecting section of the reflecting panel is crown face, which is favor for extending illumination area of road lamps.

Hemagglutination assays and hemagglutination inhibition assays were introduced in medical and virology practice more than 60 years ago. Since then, these assays have become important tools for measuring concentrations and strengths of viral cultures, the efficacy of the anti-viral immunization, and for studying the neutralizing capacity of virus-specific antibodies. The present invention comprises an improved hemagglutination inhibition assay (HAI), with at least about a 10-fold increase in sensitivity versus the traditional the HAI, to provide more accurate measurements of components in, for example, fluids from the in vitro MIMIC® system when assessing the effects of anti-viral vaccines (e.g., for seasonal influenza).

The invention provides a novel LED lamp capable of distributing light at multiple beam angles. The novel LED lamp capable of distributing the light at the multiple beam angles comprises a tubular lamp body composed of a rear cover, a lamp cup, a middle lamp body ring, an inner lamp body ring and an outer lamp body ring. The lamp cup and the rear cover are connected through a heat sink. A light source plate is installed below the heat sink. The novel LED lamp capable of distributing the light at the multiple beam angles is characterized in that a plurality of LED lamp beads are distributed on the same circumference of the light source plate in an annular array mode, a rotatable adjusting face cover is arranged on the outer lamp body ring, a lens sheet is installed in the adjusting face cover, a plurality of sets of lenses with different focal lengths are arranged on the same circumference, corresponding to the LED lamp bead array, of the lens sheet, and the same set of lenses with the same focal length can coincide with the LED lamp beads through rotation of the face cover. The novel LED lamp capable of distributing the light at the multiple beam angles has the advantages of being simple in structure, convenient to adjust, adjustable in light-emitting angle, and capable of meeting the needs for a large angle, a mediate angle and a small angle. The novel LED lamp capable of distributing the light at the multiple beam angles further has the advantages that various LED lamps or LED lamps with appointed angles can be designed according to the actual needs of users, and the demand of the users are met to the maximum extent.

The present invention relates to the preparation of a cell slide assembly for a CBA kit for detecting MOG-IgG, the CBA detection kit and its application. The preparation method of the cell slide assembly is as follows: S1: construction of MOG plasmid; S2: overexpression of MOG gene Lentiviral packaging; S3: Construction of a cell line (MOG-293T cell line) overexpressing the MOG gene; S4: Preparation of cell slide components. The kit includes cell slide assembly, anti-human IgG-FITC secondary antibody, blocking solution, PBS and mounting medium; the kit can be used for detection of MOG autoimmune IgG antibody in clinical samples. The kit of the invention has the advantages of simple operation, strong detection specificity, high sensitivity, good repeatability of experimental results, can be popularized and applied, and can be used for detection of MOG autoimmune IgG antibody in clinical samples.

The invention provides an LED display screen module with a lens and a waterproof function. The LED display screen module comprises a bottom shell, a circuit driving board arranged on the front of the bottom shell, an LED lamp bead array arranged on the circuit driving board, and a face cover arranged on the circuit driving board. The face cover is integrally covered on the front of the circuit driving circuit, four sides of the face cover are bent to wrap the four sides of the bottom shell, and waterproof rings are arranged between the bottom shell and the face cover. The bottom shell, the face cover and the waterproof rings can stop moisture from entering the interior of the LED display screen module from a display face and the four sides of the module, and enable the module to be simpler in production process, reliable in quality and high in weather resistance; the displaying angle of the module is changed and light blending effect is improved by the lens.

A method of providing a craft kit involves providing a kit including a frame with a window and a collection of beads presenting a plurality of colors and a pre-selected, fixed number of beads of each color. After providing the craft kit an image is received from a user. A bead template is generated by assigning a bead color to each of a plurality of bead positions on the template document to create a representation of the image using only beads from the collection of beads in the craft kit, and including a bead color indicator in each of said positions on the template document corresponding to the assigned bead color. Once the bead template document is created, it is transferred to the user to enable assembly of a bead array on the frame presenting the representation of the image.

In a capillary bead array, a method for retaining stably in a capillary an agent, enzyme, and fluorescent labeling agent necessary for a chemical reaction performed therein by functional particles and eluting the agent or the like in the capillary is provided. Further, a method for adsorbing and separating various target molecules contained in a sample on the functional particles based on physicochemical properties of the target molecules and eluting them is provided. By adding a solution having a temperature and composition according to the kind of a capsule shell material and an agent corresponding to core substance of a microcapsule into a capillary arrayed with the microcapsule together with probe beads, it is possible to induce the release of the agent and fill the capillary with a solution of the agent. Thus, a composition of the agent eluted in the capillary and timing of the elution can be controlled.

The invention discloses a method for detecting human myocardial troponin T through cytometric bead array. The method comprises the following three steps of: activating a bead, coupling a monoclonal antibody and detecting the human myocardial troponin T, specifically, firstly, activating the beads; secondly, preparing the monoclonal antibody of the carboxylation bead coupled mouse anti human myocardial troponin T; thirdly, capturing the human myocardial troponin T in a specimen; and finally, adding multiple antigenic and fluorescein isothiocyanate labeled donkey anti goat intravenous gamma globulin G of goat anti human myocardial troponin T, and detecting the fluorescence intensity of fluorescein isothiocyanate (FITC) on the bead on a flow cytometry to indirectly detect the content of the human myocardial troponin T in the specimen. The monoclonal antibody coupled bead, the polyclonal antibody of the goat anti human myocardial troponin T, and the fluorescein isothiocyanate labeled donkey anti goat intravenous gamma globulin (IgG) can be prepared into a commodity kit, which can detect the human myocardial troponin T at any time. The method is simple and feasible; and by the method, the human myocardial troponin T can be quickly detected with convenience and the basis for disease diagnosis is provided.

The invention discloses a probe, a primer, a detection reagent kit and a detection method for detecting hereditary hearing impairment on the basis of a floating-bead array system. The probe used for mutation of detecting hereditary hearing impairment genes is coupled and mixed with microbeads of different codes to acquire a floating-bead array; the specific primer is used for performing multiple PCR (polymerase chain reaction) amplification, products of the floating-bead array is subjected to hybridization with the floating-bead array, streptavidin R-phycoerythrin is used for color developing, and detecting results are read through a floating-bead array reading instrument. By the arrangement of the probe and the primer for detecting hereditary hearing impairment on the basis of the floating-bead array system, 20 mutation sites of the four major hereditary hearing impairment genes can be detected simultaneously, detection time can be greatly shortened, and detecting efficiency and accuracy can be improved.

Methods for proteomic screening on random protein-bead arrays by mass spec is described. Photocleavable mass tags are utilized to code a protein library (bait molecules) displayed on beads randomly arrayed in an array substrate. A library of probes (prey) can be mixed with the protein-bead array to query the array. Because mass spec can detect multiple mass tags, it is possible to rapidly identify all of the interactions resulting from this mixing.

The invention discloses an LED panel light structure. The LED panel light structure comprises a panel cover. The panel cover is square inside and outside and is chamfered, a heat dissipation rack is connected to the bottom surface of the panel cover, a dam body is defined by the heat dissipation rack along the square bottom surface of the panel cover, a square frame is formed by the inner side faces of the dam body, four rows of LED light bars are arranged along the four side faces forming the square frame, and LED light bead arrays are arranged on the LED light bars, an optical diffusion plate, a refraction plate and a reflection plate are sequentially arranged in the heat dissipation rack defining the dam body from top to bottom, a circle of step groove is formed in the lower edges of the inner side faces of the dam body defined by the heat dissipation rack, an elastic sealing sponge pad is arranged in the step groove, and the lower end of the dam body defined by the heat dissipation rack is covered with a ceramic cover plate. The four rows of LED light bars are connected with an LED protection circuit. According to the LED panel light structure, the lower end face of a heat dissipater is connected with the ceramic cover plate, the heat dissipater conducts heat to the ceramic cover plate, and thus the purpose of rapid heat dissipation is achieved; besides, a dual filter circuit is adopted, and thus LEDs can be effectively protected against damage caused by instant high-voltage breakdown.

The invention discloses a wheel disc game lamp. The wheel disc game lamp is characterized in that the game lamp which is controlled by a program and has a wheel disc swinging simulation function is provided, a multilayer round circuit board is arranged inside the game lamp, a plurality of LED lamp beads in annular distribution are arranged around the circuit board and near the lamp shade wall, after setting the wheel disc function and specifying a certain layer of lamp bead array, a user can trigger the LED lamp beads to cyclically illuminate in turns through shaking the game lamp, the wheel disc rotating effect is simulated, correspondingly, silk-screen, film pasting or engraved patterns corresponding to each LED lamp bead are arranged on a lamp shade for marking corresponding meanings in games, and the patterns can be conveniently replaced for adapting to different game rules.

The present invention comprises rugged, inexpensive, reliable, and sensitive laboratory assays of antibody-based viral neutralization activity and antibody-based viral adherence inhibition activity. The assays use inactivated, fluorescently-labeled virus, allowing the tests to be performed without extensive safety precautions. The interaction of the labeled virus with target cells is monitored using flow cytometric methods. A preferred embodiment uses simple and inexpensive flow cytometry methodologies and equipment, such as bead array readers used as simplified flow cytometers. The assays are rapid, taking no longer than a few hours and are readily conducted by a trained technician. The assays are sensitive because they use labeled viruses at low concentrations and determine neutralizing and blocking capacity of sera and antibody at low concentrations. The methods are appropriate for high-throughput screening of large panels of samples.

A capillary beads-array attractive to users is provided by immobilizing amphiphilic molecules on the surface of beads in uniform orientation and arraying the beads in a capillary formed on a soft resin. A monomolecular layer of the amphiphilic molecules is formed on the bead surface by putting beads having hydrophilic portions on the surface in water phase, dropping amphiphilic molecules containing a hydrophilic portion and a hydrophobic portion onto the water phase, pouring an oil lighter than water to form a monomolecular film of the amphiphilic molecules at an oil / water interface, capturing a single bead with the tip of a bead picker, lifting up the tip of the bead picker to allow the bead to reach the oil / water interface, and allowing to form a covalent bond between a functional group in the hydrophilic portion on the bead surface and a functional group in the hydrophilic portion of the amphiphilic molecules.

Provided are determining methods of human papillomavirus (HPV) genotypes with a high sensitivity. The method includes performing two-step PCRs on an HPV L1 gene in a sample to be analyzed as a biotin-labeled, single-stranded L1 gene, performing a hybridization reaction on the biotin-labeled, single-stranded L1 gene with a HPV genotype detection probe, reacting the hybridization reaction product with fluorescent substance combined with streptavidine, and measuring a fluorescent substance level to identify the HPV genotype. The detection method has high sensitivity enough to detect an extremely small amount of HPV in the sample. In addition, the high specificity exhibited by the detection method enables accurate diagnosis specific to HPV type.

The invention relates to a surface-enhanced Raman spectrum and a micromachining technique, in particular to a preparation method for a surface-enhanced Raman spectrum active substrate which has very high uniformity and is better enhanced. The preparation method comprises the steps of spreading single-layer polystyrene nano micro-bead arrays on a water surface; preparing spaced single-layer polystyrene nano micro-bead arrays by using a reaction plasma etching technique; and preparing the surface-enhanced Raman spectrum active substrate. The obtained surface-enhanced Raman spectrum active substrate has high uniformity and high strength at the same time. Periodic metal nano structures are arranged on a smooth silicon or glass surface. The preparation method is mainly characterized in that metal nano spacing can be adjusted and controlled and a high-activity and high-uniformity substrate can be obtained. By adopting the preparation method, the defects of the surface-enhanced Raman substrate prepared by adopting a conventional template method can be overcome and the high-quality surface-enhanced Raman substrate can be obtained by using the existing commercial materials at the same time.

Provided is a convenient process of manufacturing a two-dimensional bead array on a chip by disposing a plurality of beads immobilized with a biomolecular probe in a bead array container in a predetermined order. The bead array chip is manufactured by repeating the following operations: a container having a plurality of first channels disposed in parallel with each other and a second channel crossing therewith is retained in a container retaining portion and a capillary is moved through the second channel of the container vertically. The capillary is moved downward to suck and retain, in an end thereof, one bead stored in a storing portion of a bead storing plate and then, moved upward to the position of a desired first channel. Under this state, pure water is fed to the first passage from a water feed system and a water stream is generated by sucking the pure water by a suction pump. The bead retained in the end of the capillary is transferred by the water stream and is then, blocked by a dam disposed in the first channel, in which the bead is retained.

Within an inside of a bead chip forming a reactor flow pass therein, rinsing liquid flow passes are also provided in one body. For the purpose of promoting reaction upon a bead array, a turbulence generator or an flow separation stop guide is provided within the reactor flow pass, in which the beads are received. The bead chip is made of a PDMS (Polydimethylsiloxane: (C2H6SiO)n), thereby enabling the reactor flow pass and the rinsing liquid flow passes to be formed freely in the configurations thereof.

The invention discloses a micro ribonucleic acid detection method based on a microfluidic bead array chip and a DNA polymerase-mediated primer extension technology. According to the detection method, a micro ribonucleic acid capture probe modified by biotin is fixed to the surface of an avidin-modified microsphere through a biotin-avidin bonding method to form a functionalized microsphere; the functionalized microsphere is fixed in a micro chamber corresponding to a detection zone of a microfluidic chip so as to form the microfluidic bead array detection chip; then a sample solution containing micro ribonucleic acid is allowed to flow into the microfluidic bead array detection chip, then to flow into a DNA polymerase solution and a biotinylation extension substrate, and finally to flow into a quantum dot labeled by the avidin; and surface fluorescence of the microsphere after the micro ribonucleic acid flows into is calculated to perform qualitative operation and quantification. The method can detect the micro ribonucleic acid with high sensitivity and high specificity and achieves quantification analysis of specificity and sensitivity of the micro ribonucleic acid in a total RNA sample.

It is an object to provide a method for accurately identifying the order of probe beads, namely, the types of probes bound to the probe beads, by readily and precisely detecting the locations of all the beads in a capillary bead array using a fluorescence reading apparatus. It is also an object to significantly improve the reliability of results of biochemical or immunological inspection in which a capillary bead array is used. Probe beads 102 are arranged in a capillary 101. A solution that includes fluorescence-labeled target biomolecules is introduced into the capillary 101. A fluorescence image that includes the fluorescence spot 108 of a probe bead 105 that captured the biomolecules is obtained by the fluorescence reading apparatus. A solution that includes fluorescent material for staining all the beads is introduced into the capillary 101. A fluorescence image that includes the fluorescence spot area 114 of all the probe beads 113 is obtained by the fluorescence reading apparatus. The location and the order of the probe bead 105 that has captured the biomolecules is accurately determined by overlaying the fluorescence image that includes the fluorescence spot 108 of the probe bead that has captured the biomolecules and the fluorescence image that includes the fluorescence spot area 114 of all the probe beads 113.

The invention discloses an LED patch type ceiling lamp. The ceiling lamp is wholly circular, and comprises a lamp shell, a lamp cover, an LED module and a heat dissipating frame; the lamp shell is an arc cover surface, and is provided with a hole in the middle part; the lamp cover is an arc surface lamp cover; an arc surface end of the lamp cover is positioned in the hole of the arc cover surface; an LED bead array is arranged on the LED module; LED beads are patch type beads; the heat dissipating frame takes the shape of a circular concave surface; the LED module is fixed in the circular concave surface of the heat dissipating frame through a screw; a circular hole is formed in the bottom surface of the circular concave surface of the heat dissipating frame; an exhaust fan is arranged on the back surface of the heat dissipating frame along the edge of the circular hole; the heat dissipating frame and the lamp shell are in buckle connection; a lap of ventilation holes is arranged on the lamp shell arc surface; a smoke induction module, a gas induction module and an infrared induction module are arranged at the lower end of a circuit board of the LED module, and are connected with a processor; and the processor is connected with a bluetooth module, a warning device and a rechargeable battery. The LED patch type ceiling lamp can reduce or eliminate dangers.

The invention discloses a naked-eye three-dimensional LED display screen and a three-dimensional imaging playing system. The naked-eye three-dimensional LED display screen comprises a PCB which is provided with first foreground LED beads, second foreground LED beads and background LED beads, the first foreground LED beads and the background LED beads are arranged to form multiple first LED bead columns, the second foreground LED beads and the background LED beads are arranged to form multiple second LED bead columns, and the first LED bead columns and the second LED bead columns are arranged on the PCB to form an LED bead array; the surface of each first LED bead column and the surface of each second LED bead column are each provided with a transparent resin layer unit, the cross sections of the transparent resin layer units are symmetrical in the direction perpendicular to the LED bead array, and the transparent resin layer units are arranged to form a transparent resin layer to cover the LED bead array. Accordingly, videos of the left foreground, the right foreground and the background are displayed in an overlapped mode by setting a light field difference and the prismatic transparent resin layer, and then naked-eye three-dimensional viewing is achieved.

The invention relates to bead stringing equipment, in particular to bead stringing equipment for ornament processing. The bead stringing equipment for ornament processing realizes that beads can be placed without depending on hands and can be stringed. The bead stringing equipment for ornament processing comprises an installation frame, an installation cover, an arrangement device, a collection hopper, a discharging device and the like. The installation cover is installed on the installation frame, the arrangement device for arranging beads in a rotating mode is arranged between the installation cover and the installation frame, the collection hopper is installed above the installation cover, and the discharging device for discharging the beads in a pulling mode is arranged between the collection hopper and the installation frame. According to the bead stringing equipment, beads can be arrayed through the arrangement device, so that beads can be stringed better, the beads can be conveniently placed on the arrangement device through the discharging device, the bead arraying speed is increased, and the bead stringing equipment is also convenient to use.

The invention discloses a cochlear implant magnet and a device. The cochlear implant magnet comprises an inner magnet and an outer magnet, wherein the inner magnet comprises an inner magnet upper cover, an inner magnetic bead array, an inner magnetic bead holder and an inner magnet lower cover arranged from top to bottom; the inner magnetic bead array comprises a plurality of inner magnetic beads with N and S poles; the inner magnetic bead is ball-shaped and is arranged in the inner magnetic bead holder; the inner magnetic bead array and the inner magnetic bead holder are in box-shaped space formed when the inner magnet upper cover and the inner magnet lower cover are covered, the inner part has no extra space, and the inner magnetic bead array is held in the inner magnetic bead holder. The magnet can rotate flexibly on the magnetic bead holder with a self-lubrication function according to changes of the direction of the outer magnetic field, the N pole and the S pole of the implant magnet can be switched freely in the space direction, and the cochlear implant does not need to be taken out in the case of inspection in a strong magnetic field such as MRI.

The invention discloses an LED display, a display device and a display system. The LED display comprises an LED lamp bead array composed of a number of LED lamp beads. The LED lamp bead array comprises left image LED lamp bead arrays and right image LED lamp bead arrays. The left image LED lamp bead arrays and the right image LED lamp bead arrays are arranged at intervals according to a preset arrangement rule. Each wavelength of RGB three primary colors corresponding to the left image LED lamp bead arrays and each wavelength of RGB three primary colors corresponding to the right image LED lamp bead arrays are not the same. According to the invention, without increasing the cost of matching 3D glasses, the technical problem of poor display performance of the existing 3D display technology is solved.