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36 results about "Platelet antibody" patented technology

If you have platelet antibodies in your blood, it means your immune system is mistakenly creating antibodies to attack the platelets in your blood. People with idiopathic thrombocytopenic purpura or systemic lupus erythematosus may have platelet antibodies.

Flow micro-sphere method for detecting plastocyte specificity immune body

InactiveCN101246173AIncreased sensitivityContribute to basic experimental researchMaterial analysisHuman plateletMicrosphere
The invention provides a method belonging to the field of cytometric bead array with high sensitivity for testing platelet specific antibody based on platelet basic experimental study. Platelet lysate is used to incubate the microsphere which is coated with platelet membrane glycoprotein monoclonal antibody, then goat-anti-human IgG polyclonal antibody labeled with phycoerythrin is added, which is analyzed in cytometric bead array. If autoantibodies exist on the surface of platelet, 'microsphere-platelet membrane glycoprotein monoclonal antibody-platelet membrane glycoprotein autoantibodies-goat-anti-human IgG polyclonal antibody labeled with phycoerythrin' complex structure is formed, the fluorescence intensity of the test microsphere is enhanced. The invention is easy to operate, and has mature technology, high sensitivity for testing platelet autoantibodies, which is good for basic experimental study of platelet antibody.
Owner:侯明 +2

Immunofluorescence chromatography test strip for detecting platelet antibody and detection method of platelet antibody

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.
Owner:SUZHOU GUOKE MEDICAL TECH DEV CO LTD

Platelet antibody detection kit and use method thereof

The invention provides a platelet antibody detection kit and a use method thereof. The platelet antibody detection kit comprises a detection plate (1), six gel tubes (2) and an opening sealing film (3), wherein the six gel tubes (2) are arranged at the top end of the detection plate (1) in a left and right arranging way; the opening sealing film (3) is arranged above the detection plate (1), and covers the top ends of the six gel tubes (2); each of six gel tubes (2) contains an anti-human globulin reagent and sephadex; the first gel tube and the sixth gel tube are respectively a positive contrast tube and a negative contrast tube, and the second gel tube, the third gel tube and the fourth gel tube are reaction tubes. The platelet antibody detection kit has the advantages that anti-human IgG and IgG sensitized erythrocyte are used as indication systems to replace anti-human IgG-coated aldehydated erythrocyte, the detection result is clear and accurate, and the repeatability is strong; the effect that detection result can be accurately judged by the naked eye and automatic robot at the same time, and the detection result is easily standardized.
Owner:北京乐普诊断科技股份有限公司

Platelet antibody detection method based on nano material and platelet preservation method

The invention relates to a platelet antibody detection method based on a nano material and a platelet preservation method. In the invention, nano composite particles prepared by wrapping PLGA with platelet membrane protein are applied to platelet antibody detection for the first time, which proves that the platelet antibody can be accurately detected by taking human-derived serum containing an anti-platelet antibody as a detection object, and good sensitivity is possessed. In the invention, improvement of clinical diagnosis of platelet immune abnormal diseases is facilitated, matched plateletsare provided for patients, a clinical infusion effect of the platelets is improved, clinical blood transfusion quality is increased, and precious blood resources are saved for the society at the sametime.
Owner:SHANGHAI BLOOD CENT

Cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia

InactiveCN107422111AIncreased sensitivityContribute to clinical trial researchDisease diagnosisHuman plateletMicrosphere
The invention belongs to blood platelet clinical test and research and relates to a cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia. The cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia is high in sensitivity and specificity. The method comprises encapsulating beads through anti-human platelet membrane glycoprotein monoclonal antibodies, incubating platelet lysate and the encapsulated beads, and then adding in phycoerythrin marked goat antihuman immune globulin polyclonal antibodies to perform flow cytometry. If autoantibodies exist on the surface of platelets, a bead-platelet membrane glycoprotein monoclonal antibody-platelet membrane glycoprotein autoantibody-phycoerythrin marked goat antihuman immune globulin polyclonal antibody composite structure can be formed and embodied as increase of fluorescence intensity of detecting beads. The cytometric bead method for detecting blood platelet autoantibodies of immune thrombocytopenia is convenient to operate, mature in technology, high in sensitivity of blood platelet autoantibody detection and conductive to fundamental experimental research on platelet antibodies.
Owner:冀学斌

Full automatic platelet antibody detecting device

The invention discloses a fully automatic platelet antibody detecting device. The fully automatic platelet antibody detecting device comprises an outer shell body, a sample loading arm module, an outer ring rotating disc device, a centrifuge, an incubation module, a heat dissipation module, a sample carrier, a reagent carrier, a reading camera, a scanning head, a scanning head supporting plate anda driving device fixing seat. The driving device fixing seat is fixed to the bottom plate of the outer shell body, a driving device is installed on the driving device fixing seat, the outer ring rotating disc device is installed on the driving device, the outer ring rotating disc is in a hollow structure, the centrifuge is installed in the hollow structure of the outer ring rotating disc, the incubation module and the heat dissipation module are arranged in the centrifuge, the sample carrier and the reagent carrier are fixed in thecircumferential direction of the outer ring rotating disc. According to the fully automatic platelet antibody detecting device, full automation of processes such asreagent distribution, sample distribution, oscillating mixing, incubation, centrifugation, washingand interpretation can be achieved, labor intensity of experimenters is greatly reduced, and the inspecting efficiency is improved.
Owner:深圳市爱康生物科技股份有限公司

Water-soluble adhesive rapidly separating method for detecting surfactant molecules of platelet membrane

InactiveCN106840823AAvoid cumbersome experimental proceduresEasy to operatePreparing sample for investigationAdhesiveFluorescence
The invention discloses a water-soluble adhesive rapidly separating method for detecting surfactant molecules of s platelet membrane, comprising steps of: mixing a platelet to be detected and a fluorescently-labeled antibody, and adding same to a water-soluble adhesive chromatography medium to obtain a mixed liquid; then performing centrifugal layering on ttheo mixed liquid and discarding an upper layer; and after adding a buffer solution for resuspending, obtaining a result. By means of combining said water-soluble adhesive chromatography and low-speed centrifugation, the method provided by the invention may separate the antibodies that are not combined with the platelet and other soluble protein molecules in a reaction liquid from the platelet-antibody compounds, which avoids tedious experiment procedures of multiple washing required during the operation process of a conventional method, greatly simplifies operation steps, saves operation time, and improves detection efficiency.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Immunofluorescence chromatography test strip and detection method for platelet antibody detection

The invention discloses an immunofluorescence chromatography test strip for detecting a platelet antibody and a detection method of the platelet antibody. The immunofluorescence chromatography test strip comprises a reaction film, a sample pad, an adsorption pad and a bottom plate, wherein the reaction film is placed on the bottom plate; a detection belt and a quality control belt are fixed onto the reaction film; the sample pad is overlapped with part of one side of the reaction film and placed on the reaction film and the bottom plate; the absorption pad is overlapped with part of the other side of the reaction film and placed on the reaction film and bottom plate; a sampling point is arranged on each of two sides of the sample pad; sample and fluorescence labeled secondary antibodies are dropped and washed; and after the reaction is done, the reaction result is indicated through fluorescein isothiocyanate labeled secondary antibodies. Due to the mode, according to the immunofluorescence chromatography test strip for detecting the platelet antibody and the detection method disclosed by the invention, the detection process is simple, efficient, accurate, low in cost and practical; semi-quantitative or quantitative detection can be realized; safe, efficient and scientific platelet transfusion in clinic can be guaranteed; and the precious platelet resources also can be saved.
Owner:SUZHOU GUOKE MEDICAL TECH DEV CO LTD

Method for detecting platelet antibody specificity by using flow cytometry and detection kit

PendingCN111175488AQuick checkThe result is accurateMaterial analysisPlatelet-specific antibodyPlatelet antigen Zw
The invention provides a method for detecting platelet antibody specificity by using flow cytometry. The method comprises the following steps: (1) preparing platelet antigen specific fluorescent microspheres; (2) preparing a detection antibody; (3) preparing a platelet reference substance; (4) detecting a reaction system; and (5) detecting a sample to be detected. The invention also aims at providing a detection kit for detecting the specificity of the platelet antibody by applying the flow cytometry. The kit comprises platelet antigen specific fluorescent microspheres obtained by coating thesurfaces of different fluorescent microspheres with different anti-platelet specific glycoprotein antibodies, a microsphere buffer solution, a platelet reference substance, a platelet lysate, a cleaning solution, calibration microspheres and a detection antibody. The invention discloses a platelet specific antibody detection method. In the prior art, the platelet antibody detection mainly aims atthe platelet antibody of the detected person; compared with the prior art, the method has the advantages that the problem of free platelet antibodies in blood plasma or blood serum is solved, the typeof the platelet specific antigen (HPA) of the antibody existing in a detected person can be further determined, and the method is small in sample use amount, more accurate in result, simple in step,easy to operate and higher in efficiency.
Owner:天津美瑞特医疗科技有限公司

Traditional Chinese medicine composition for inhibiting and removing platelet antibody and curing idiopathic thrombocytopenic purpura

The invention provides a traditional Chinese medicine composition for inhibiting and removing aplatelet antibody and curing idiopathic thrombocytopenic purpura, wherein honeysuckle capable of clearing away heat and toxic materials and good at removing blood poisonis is taken as a monarch drug in a prescription, and is used to remove the platelet antibody in the blood. Radix rehmanniae, cortex moutan, red peony root and ligusticum wallichii are taken as an adjuvant drug, and are used to cool blood, clear heat, improve the internal environment of blood heat of a human body, and prevent and control the internal base for the generation of a blood platelet; lithospermum, madder, nodus nelumbinis rhizomatis charcoal and radix notoginseng powder are used to cool blood, remove blood stasis, stop bleeding and improve the signs and symptoms of bleeding; divaricate saposhnikovia root is used to dispel wind and clear heat, and is compatible with the honeysuckle to avoid relapse of the disease, caused by the reason that wind heat enters the interior. Liquorice is a conductant drug, relaxes tension, detoxifies, and coordinates the drug actions of the prescription. The various medicines in the prescription are combined, the prescription is simple but complete, is in great accuracy and hits the pathogenesis, and the various medicines play the roles of detoxifying, cooling blood, dispersing blood stasis, removing ecchymosis and nourishing spleen and kidney together, so that the bleeding of an ITP patient is reduced, and the various clinical symptoms are further alleviated.
Owner:SHANDONG PROVINCIAL HOSPITAL

Cleaning solution for washing blood platelet antibody detection kit microporous plate

The invention discloses a cleaning solution for washing a blood platelet antibody detection kit microporous plate. The cleaning solution mainly comprises sodium dihydrogen phosphate, disodium hydrogenphosphate, Tween 20 and an appropriate amount of purified water and is characterized in that the pH of the cleaning solution is 6.5-7.5 at 25 DEG C, and each 200mL of purified water contains 0.2-0.8gof sodium dihydrogen phosphate, 1.0-2.5g of disodium hydrogen phosphate, 0.5-1.5mL of Tween 20 and 7-9g of sodium chloride; the cleaning solution is used for washing the microporous plate after beingdiluted by 25 times through the purified water. Compared with a conventional cleaning solution, the cleaning solution has the advantages that negative cell button lunar halo can be eliminated evidently, and the cleaning solution is simple to prepare and low in cost; the shelf life of the unopened cleaning solution at room temperature of 10-30 DEG C is four months; the shelf life of the opened cleaning solution is two months.
Owner:江苏中济万泰生物医药有限公司

Internet-based full-automatic platelet antibody detection system and use method thereof

The invention discloses an internet-based full-automatic platelet antibody detection system and a use method thereof, and belongs to the technical field of medical instruments. A full-automatic platelet antibody detection system based on the Internet comprises a machine body, a control system is arranged in the machine body, a partition plate is arranged in the machine body and divides the machine body into an upper-layer space and a lower-layer space, a centrifugal machine is arranged in the lower-layer space, and a rotating shaft connected with the centrifugal machine is arranged in the upper-layer space. The rotating shaft is provided with a plurality of detection parts which are distributed circumferentially, each detection part comprises two hanging basket assemblies which are symmetrically arranged on the rotating shaft, and the rotating shaft is internally provided with a balance rod; the device can be suitable for inspection of different numbers of detection cards at the same time, balance of the symmetrical hanging basket assemblies on the two sides can be rapidly adjusted, operation is very convenient, the detection work efficiency is improved, practicability is high, the hanging baskets for containing the detection cards are always horizontally placed before and after use, and rapid taking and placing of the detection cards are facilitated.
Owner:付小斌
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