The invention belongs to the technical field of platelet antibody detection, and in particular relates to an anti-sieve cell suitable for platelet antibody detection, its preparation method and application. The anti-screening cells were prepared by the following method: 1) 3 portions of O-type platelets that had been screened to confirm the antigen profile were mixed in equal volumes, washed with platelet cleaning solution, centrifuged, the supernatant was discarded, and the platelet precipitate was retained; 2) Resuspend the platelet pellet from step 1) in platelet loading solution and adjust the cell concentration to (1.0‑2.0)×10 10 cells / ml, shaking; 3) After shaking, use platelet cleaning solution to wash again. After cleaning, use platelet storage solution to resuspend the pellet, and adjust the cell concentration to (2.0‑5.0)×10 7 cells / ml to obtain anti-sieve cells suitable for platelet antibody detection. The platelet anti-sieve cells prepared by the method can be applied to platelet antibody detection, have a long storage time, are convenient to use, and have good antigen preservation effect.