13 results about "Platelet antibody" patented technology

The invention relates to the technical field of biological detection, and provides a kit and a method for rapidly detecting a platelet antibody by utilizing a phytolectin solid-phase adsorption method. The detection kit provided by the invention comprises a reaction plate which is coated with phytolectin and adsorbs platelets. According to the method, the platelet of multiple persons is adsorbed by utilizing the non-specific adsorption capacity of the phytolectin, and the adsorbed platelet is directly freeze-dried to the bottom of a microwell plate. During use, a detection result can be obtained only by adding detected plasma / serum into the reaction plate for incubation and then adding an anti-human globule reagent and indicating red blood cells, so that intermediate links of detection are reduced, and standardization and automation of detection are facilitated.

The invention discloses a platelet antibody detection kit and application thereof. Belongs to the technical field of detection kits. The kit comprises a microcolumn gel card, an enzyme-labeled secondary antibody, an enzyme-labeled secondary antibody diluent, a 20x washing solution, a developing solution, a fresh platelet treatment solution, a platelet buffer solution, a platelet preparation solution, a freeze-dried platelet reconstitution fluid, freeze-dried platelets and a negative control. The platelet antibody detection kit disclosed by the invention has the beneficial effects that the platelet antibody detection kit disclosed by the invention enables cross-matching of platelets to be possible; according to the invention, a micro-column gel immunoassay technology is combined with an enzyme immunolabeling technology, so that the limitation that only agglutination reaction can be carried out in a micro-column gel method is solved; according to the reaction card washing equipment disclosed by the invention, semi-automatic operation of micro-column gel method detection is realized; in the plasma, triglyceride is less than or equal to 5mg / mL, bilirubin is less than or equal to 20mg / dL, and hemoglobin is less than or equal to 5mg / mL, so that the result is not influenced; the kit has the advantages of good stability, long effective period, high sensitivity, strong specificity and simple operation.

The present invention relates to a fully automatic platelet antibody detection analyzer and a method for using the same. The analyzer comprises a control system, a circuit board, a base, a pipette tip storage module, a sample storage module, a gel card storage puncture module, a test waste discard module, a centrifugal incubation module, a reagent storage module, a card clamping manipulator module, a detection module, a beam module and a sample loading arm slide rail module. The method for using the analyzer comprises the following steps: placing a pipette tip, a platelet suspension, a diluent, a fluorescent reagent, a sample tube and a gel card into corresponding positions, and setting detection sample parameters; puncturing a sealing film of the gel card; adding the platelet suspension, the diluent, the fluorescent reagent and a plasma sample into the gel card; after incubating and centrifuging the gel card, taking photos for interpretation and detecting the fluorescence intensity, and providing qualitative and quantitative detection results. The gel card is discarded after the detection is completed, and this process is repeated until all the detections are completed. The present invention is helpful for clinical diagnosis of platelet immune diseases and improving the efficacy of platelet transfusion.

The present invention discloses a fully automatic platelet antibody detection device, which includes an outer housing, a sample adding arm module, an outer ring turntable device, a centrifuge, an incubation module, a heat dissipation module, a sample carrier, a reagent carrier, a reading camera, a scanning head, a scanning head support plate, and a driving device fixing seat. The driving device fixing seat is fixed on the bottom plate of the outer housing, and a driving device is installed on the driving device fixing seat. The outer ring turntable device is installed on the driving device. The outer ring turntable device is of a hollow structure. The centrifuge is installed inside the hollow structure of the outer ring turntable device. The incubation module and the heat dissipation module are arranged inside the centrifuge. The sample carrier and the reagent carrier are fixed circumferentially on the outer ring turntable device. The fully automatic platelet antibody detection device provided by the present invention can realize the complete automation of processes such as reagent dispensing, sample dispensing, oscillating and mixing, incubation, centrifugation, washing, and reading in platelet antibody detection, greatly reducing the labor intensity of experimental personnel and improving the detection efficiency.

The invention relates to the field of biological detection, and particularly discloses a method and a kit for determining platelet antibodies and cross-matching types by a chemiluminescence method. The detection method comprises the following steps: firstly, adding tool platelets / donor platelets into a reaction hole, adding a sample to be detected and magnetic bead particles coated with platelet antibodies into the reaction hole, and incubating to obtain an immune complex; mixing the immune complex with an acridinium ester labeled anti-human IgG antibody, and incubating to obtain a reaction complex; and mixing the reaction compound with a pre-excitation liquid and an excitation liquid of acridinium ester in sequence, carrying out a chemiluminescence reaction, and calculating the concentration of the platelet antibody in the sample to be detected. The kit not only can be used for effectively screening platelet antibodies in a to-be-detected plasma sample, but also can be used for carrying out cross-matching detection before platelet infusion, so that a more accurate basis is provided for evaluating the platelet infusion effect and formulating a treatment scheme.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

The application discloses an anti-platelet GPIb-IX antibody and application thereof, and belongs to the technical field of biological medicines. The application selects the starting signal protein GPIb-IX of arterial thrombosis as a target, and prepares an anti-human GPIb-IX humanized antibody. Specifically, human platelets are used as antigens to immunize mice, and a mouse-derived anti-human GPIb-IX antibody is obtained through screening and preparation, a humanized anti-GPIb-IX antibody ATHA is constructed by mutating a human-derived sequence, the heavy chain variable region of the ATHA comprises a VH-CDR3 shown in SEQ ID NO. 1, and the light chain variable region comprises a VL-CDR3 shown in SEQ ID NO. 2. The Fab segment of the ATHA can effectively inhibit platelet activation mediated by the GPIb-IX receptor, inhibit thrombosis in the body without accompanying side effects such as platelet reduction and bleeding, and has the characteristics of high specificity, good safety and small side effects. Meanwhile, the ATHA can remove platelets. Therefore, the ATHA-Fab has clinical value as a new anti-thrombotic drug, and can be used for treating diseases related to arterial thrombosis. The ATHA can remove platelets, and can be used for treating diseases with increased platelet quantity or removing platelets in the body.

The utility model relates to a washable in-vitro diagnostic reagent card, which belongs to the technical field of medical instruments and comprises a first clamping plate, a plurality of reagent cylinders, a second clamping plate and a plurality of blocking cylinders, a supporting plate is vertically fixed at the top side end of the first clamping plate, and a plurality of through inserting and fixing holes are formed in the supporting plate and the first clamping plate at intervals; the plurality of reagent cylinders are respectively inserted and fixed in the plurality of inserting and fixing holes, and the bottom ends of the reagent cylinders penetrate out of the corresponding inserting and fixing holes; the second clamping plate is positioned below the first clamping plate in parallel; the bottom ends of the blocking cylinders are all closed ends, and the closed ends of the blocking cylinders are fixed to the top side ends of the second clamping plates correspondingly; and the extending ends of the plurality of reagent cylinders are detachably inserted into the plurality of blocking cylinders respectively. According to the in-vitro diagnostic reagent card, a traditional integrated in-vitro diagnostic reagent card is designed into the first clamping plate and the second clamping plate which can be separated, and the reagent cylinder is of a hollow structure after being separated from the blocking cylinder, so that the reagent cylinder can be washed by using washing liquid from the upper part and the lower part, and the requirement of repeatedly washing the reagent cylinder in platelet antibody detection is met.

The invention discloses a peripheral blood sample stabilizing solution for inhibiting platelet activation through a multi-target synergistic effect and also discloses a composite anticoagulant formula containing the stabilizing solution. The composite anticoagulant formula comprises three parts, namely a stabilizing solution, a platelet antibody detection reagent and a fixing agent. The platelet can be protected or platelet activation can be reversibly inhibited, on one hand, the detection time of the platelet resting state can be prolonged, on the other hand, the activation, agglutination and adhesion functions of the platelet are reserved, and convenient conditions are provided for research and detection of in-vitro stimulation of the platelet.

The utility model belongs to the technical field of platelet antibody detection devices, and discloses an intelligent platelet antibody detection device which comprises a rack, a sample carrier, a driving assembly and a detection module, and the rack forms a main body supporting structure of the device; the sample carrying frame is arranged on the rack and comprises a bottom frame piece and a carrying piece; first convex lugs are fixed to the four corners of the bottom frame part, dampers are vertically fixed to the tops of the first convex lugs, buffer springs are connected to the outer surfaces of the dampers in a sleeving mode, mounting parts are fixed to the tops of the dampers, and locking nuts are arranged on the outer surfaces of the tops of the mounting parts in a threaded sleeving mode. According to the utility model, quick separation and fixation of the carrying piece and the underframe piece are realized, and carrying pieces with different specifications can be replaced without disassembling the whole component. In the moving process of the sample carrier, the buffer spring and the damper can effectively absorb vibration generated by transmission, and the liquid sample in the sample test tube is prevented from shaking left and right.

The utility model discloses a kind of platelet antibody detection with impurity removal device, including collection mechanism, liquid outlet mechanism and impurity removal mechanism, the collection mechanism includes tube body, with the liquid inlet pipe of the tube body connection and intercommunication, with the wall joint of the tube body inner disc, liquid outlet mechanism, the liquid outlet mechanism includes the slope surface being opened in the top of the disc, is inserted in the plugboard of the slope surface bottom and with the disc plug connection.The utility model in, when carrying out impurity removal operation, pull out plugboard, make the opening on plugboard come to the top of notch, then sample flows into the notch inside close to tube body top end from slope surface position, then tube body is horizontally placed, so that sample flows into another notch along arc-shaped groove, then repeat the step, until sample completely flows out, in this process, every time sample passes a notch, impurity in sample will be intercepted, thereby complete the purification of sample, ensure the accuracy of detection result.

The invention discloses an anti-platelet antibody detection kit based on magnetic particle chemiluminescence and application, and relates to the technical field of blood detection. Comprising a reagent M, which comprises magnetic particles coated with recombinant antigen proteins GPIIb / IIIa, GPIa / IIa, GPIb / IX and HLA antigen at the same time, and a magnetic particle protection solution containing Ca < 2 + >; the R1 reagent comprises a buffer solution; the R2 reagent comprises an anti-human IgG antibody marked by an enzyme or a luminescence agent; wherein the magnetic particles are treated by the following steps: adding EDC, NHS and NH2-PEG6000-COOH into the magnetic particles, and carrying out oscillatory reaction for more than 4 hours; according to the kit, magnetic particle immobilization coating is combined with a chemiluminescence immunoassay signal amplification system for detection, multiple synchronous or independent detection of multiple antigens is achieved, the detection range is wide, and the kit has good sensitivity, specificity and stability and good detection repeatability.

The invention discloses an anti-platelet GPIb-IX antibody and application thereof, and belongs to the technical field of biological medicine. According to the invention, an initial signal egg GPIb-IX formed by arterial thrombosis is selected as a target spot to prepare the anti-human GPIb-IX humanized antibody. Specifically, human platelets are used as antigens to immunize mice, a mouse anti-human GPIb-IX antibody is obtained through screening and preparation, an adult sequence is mutated to construct the anti-GPIb-IX humanized antibody ATHA, a heavy chain variable region of the antibody comprises VH-CDR3 as shown in SEQ ID NO.1, and a light chain variable region of the antibody comprises VL-CDR3 as shown in SEQ ID NO.2. The Fab segment of the ATHA can effectively inhibit GPIb-IX receptor mediated platelet activation, thrombus formation is inhibited in vivo, meanwhile, the side effects of thrombocytopenia and bleeding are avoided, and the ATHA has the advantages of being high in specificity, good in safety and small in side effect. Meanwhile, ATHA can remove platelets. Therefore, ATHA-Fab has a clinical value of becoming a new antithrombotic drug, and can be used for treating diseases related to arterial thrombosis. ATHA can clear platelets, and can be used for treating platelet number increase diseases or clearing platelets in vivo.