5 results about "Platelet antibody" patented technology

The invention discloses a platelet antibody detection kit and application thereof. Belongs to the technical field of detection kits. The kit comprises a microcolumn gel card, an enzyme-labeled secondary antibody, an enzyme-labeled secondary antibody diluent, a 20x washing solution, a developing solution, a fresh platelet treatment solution, a platelet buffer solution, a platelet preparation solution, a freeze-dried platelet reconstitution fluid, freeze-dried platelets and a negative control. The platelet antibody detection kit disclosed by the invention has the beneficial effects that the platelet antibody detection kit disclosed by the invention enables cross-matching of platelets to be possible; according to the invention, a micro-column gel immunoassay technology is combined with an enzyme immunolabeling technology, so that the limitation that only agglutination reaction can be carried out in a micro-column gel method is solved; according to the reaction card washing equipment disclosed by the invention, semi-automatic operation of micro-column gel method detection is realized; in the plasma, triglyceride is less than or equal to 5mg / mL, bilirubin is less than or equal to 20mg / dL, and hemoglobin is less than or equal to 5mg / mL, so that the result is not influenced; the kit has the advantages of good stability, long effective period, high sensitivity, strong specificity and simple operation.

The application discloses a platelet antibody detection and cross matching blocking chromatography kit and a detection method thereof, wherein the kit comprises a U-shaped microwell plate, a chromatography medium for separating platelet immune complexes from non-cell particle components under the action of centrifugal force, freeze-dried platelets, a platelet diluent, a platelet fluorescent staining solution comprising fluoresceinized platelet antibodies specifically labeled for platelets, biotin-labeled anti-human globulin for specifically binding with antibodies bound to the surface of the platelets, avidinized fluorescein for coupling with the biotin-labeled anti-human globulin through a biotin-avidin specific binding system, negative control serum / plasma and positive control serum / plasma. The application combines the blocking chromatography technology with a high-sensitivity flow immunofluorescence method to construct an efficient and accurate platelet antibody detection and cross matching system, which is high in specificity and sensitivity, simple in operation and stable in detection results.

The application discloses an anti-platelet GPIb-IX antibody and application thereof, and belongs to the technical field of biological medicines. The application selects the starting signal protein GPIb-IX of arterial thrombosis as a target, and prepares an anti-human GPIb-IX humanized antibody. Specifically, human platelets are used as antigens to immunize mice, and a mouse-derived anti-human GPIb-IX antibody is obtained through screening and preparation, a humanized anti-GPIb-IX antibody ATHA is constructed by mutating a human-derived sequence, the heavy chain variable region of the ATHA comprises a VH-CDR3 shown in SEQ ID NO. 1, and the light chain variable region comprises a VL-CDR3 shown in SEQ ID NO. 2. The Fab segment of the ATHA can effectively inhibit platelet activation mediated by the GPIb-IX receptor, inhibit thrombosis in the body without accompanying side effects such as platelet reduction and bleeding, and has the characteristics of high specificity, good safety and small side effects. Meanwhile, the ATHA can remove platelets. Therefore, the ATHA-Fab has clinical value as a new anti-thrombotic drug, and can be used for treating diseases related to arterial thrombosis. The ATHA can remove platelets, and can be used for treating diseases with increased platelet quantity or removing platelets in the body.

The utility model belongs to the technical field of platelet antibody detection devices, and discloses an intelligent platelet antibody detection device which comprises a rack, a sample carrier, a driving assembly and a detection module, and the rack forms a main body supporting structure of the device; the sample carrying frame is arranged on the rack and comprises a bottom frame piece and a carrying piece; first convex lugs are fixed to the four corners of the bottom frame part, dampers are vertically fixed to the tops of the first convex lugs, buffer springs are connected to the outer surfaces of the dampers in a sleeving mode, mounting parts are fixed to the tops of the dampers, and locking nuts are arranged on the outer surfaces of the tops of the mounting parts in a threaded sleeving mode. According to the utility model, quick separation and fixation of the carrying piece and the underframe piece are realized, and carrying pieces with different specifications can be replaced without disassembling the whole component. In the moving process of the sample carrier, the buffer spring and the damper can effectively absorb vibration generated by transmission, and the liquid sample in the sample test tube is prevented from shaking left and right.

The utility model discloses a kind of platelet antibody detection with impurity removal device, including collection mechanism, liquid outlet mechanism and impurity removal mechanism, the collection mechanism includes tube body, with the liquid inlet pipe of the tube body connection and intercommunication, with the wall joint of the tube body inner disc, liquid outlet mechanism, the liquid outlet mechanism includes the slope surface being opened in the top of the disc, is inserted in the plugboard of the slope surface bottom and with the disc plug connection.The utility model in, when carrying out impurity removal operation, pull out plugboard, make the opening on plugboard come to the top of notch, then sample flows into the notch inside close to tube body top end from slope surface position, then tube body is horizontally placed, so that sample flows into another notch along arc-shaped groove, then repeat the step, until sample completely flows out, in this process, every time sample passes a notch, impurity in sample will be intercepted, thereby complete the purification of sample, ensure the accuracy of detection result.