297 results about "Hemoglobin hb" patented technology

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The invention discloses a wound healing state evaluation method and system based on artificial intelligence, and belongs to the field of wound healing evaluation. The method comprises the following steps: integrating medical record data and wound physiological parameters, and extracting relevance between a medication history and an infection type to form a multi-dimensional pathological factor; hemoglobin oxygenation, blood flow and metabolic heat are subjected to cross-period correlation analysis to construct dynamic physiological time-space information, pathological factors and the physiological time-space information are fused into a heterogeneous graph, nodes comprise drug intensity quantized values, infection risk levels and microcirculation fluctuation values, and edge weights are determined by the time sequence matching degree of drug doses and blood flow changes; based on the node weight and the edge association relationship, the treatment time and the physiological parameter acquisition period are aligned to generate a spatio-temporal evolution matrix, the spatial distribution of the infection risk level and the metabolic heat value characteristics are combined, the dynamic association of the drug action intensity and the microcirculation fluctuation is analyzed, and wound healing evaluation information is generated. The accuracy of wound healing state evaluation can be improved.

The invention discloses a non-invasive wearable vascular arteriosclerosis assessment system and method based on multi-mode pulse wave signal fusion, and belongs to the field of vascular function assessment. The system comprises a distributed acquisition front end, a butterfly-shaped flexible substrate, an integrated double-accelerometer, a four-wavelength LED (530nm / 660nm superficial blood vessel measurement), a 810nm / 940nm deep artery measurement device, a double-photodiode, a double-impedance electrode and a pressure sensor. The central integrated control end processes a multi-mode signal and realizes pressure-LED current closed-loop control; the upper computer terminal extracts the multi-modal pulse wave time difference of the limbs and the neck, the local and regional pulse wave conduction velocity and the blood oxygen hemoglobin change parameters induced by the standardized posture, the dimension is reduced to 30 through principal component analysis, then the parameters are input into the random forest regression model, and the vascular elasticity index is output. Signal stability is improved through flexible fitting, single-source limitation is solved through multi-modal fusion, signal quality is guaranteed through closed-loop control, sensitivity is enhanced through dynamic physiological excitation, and accuracy and universality of arteriosclerosis assessment are improved.

The invention discloses an endoscope image processing method based on oxyhemoglobin saturation and multiple central wavelengths, and relates to the field of medical image processing.The method comprises the steps that a first initial image signal, a second initial image signal, a third initial image signal and a fourth initial image signal are obtained; calculating oxyhemoglobin concentration information of the target part according to the second initial image signal and the third initial image signal; calculating deoxidized hemoglobin concentration information of the target part according to the second initial image signal and the fourth initial image signal; calculating the oxyhemoglobin saturation of the target part according to the oxyhemoglobin concentration information and the deoxidized hemoglobin concentration information; performing brightness enhancement on the second initial image signal based on the oxyhemoglobin saturation to obtain a second enhanced image signal; and generating a target image according to the second enhanced image signal, the first initial image signal and the third initial image signal, wherein the target image is used for identifying the bleeding position of the target part.

The invention relates to a cognitive state classification method based on EEG-fNIRS spatio-temporal fusion features, which comprises the following steps of: setting an experiment according to a mental calculation experiment normal form, and synchronously acquiring EEG data and fNIRS data of a tested mental calculation task; preprocessing the two collected data to obtain electroencephalogram signal data and hemoglobin concentration change data; eEG signal data and hemoglobin concentration change data are taken, data enhancement is carried out through a time window, then the data are sent to a space-time fusion network, EEG features, oxyhemoglobin HBO features and deoxyhemoglobin HBR features are obtained, fusion features are obtained through fusion, and an EEG classification result, an fNIRS classification result and a fusion feature classification result are obtained. According to the method, the characteristics of the EEG signal and the fNIRS signal are effectively combined, the overfitting problem in modal deep learning is relieved, and cognitive state classification can be accurately carried out.

The invention discloses a method for biological high-density synthesis of retinaldehyde and a synthetic strain thereof. The synthetic strain is obtained by expressing ATP citrate lyase ACL, acetyl-CoA synthase ACS, choline kinase CK, inositol polyphosphate kinase IPK and vitreoscilla hemoglobin VHb in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified bacterial strain PP-B2C. The retinaldehyde production performance of the recombinant strain is verified on the basis of a precursor supply pathway, an IUP pathway and a dissolved oxygen level, and the retinaldehyde production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of retinaldehyde can be better promoted, and the retinaldehyde can be efficiently synthesized by the engineering strain by utilizing glucose and methanol through amplification fermentation of a 5L fermentation tank, so that the recombinant pichia pastoris is beneficial to industrial production.

The invention belongs to the technical field of biology, and particularly discloses a high-density fermentation method and application of recombinant spider silk protein, and the high-density fermentation method comprises the following steps: firstly, transforming an expression vector carrying spider silk protein genes into modified pichia pastoris cells to obtain a strain; the activated strain is subjected to expanding culture, high-density fermentation culture and purification, and recombinant spider silk protein is obtained; wherein the transformation means of the pichia pastoris cell is to integrate Vitreoscilla hemoglobin Vhb gene into the pichia pastoris cell by utilizing a gene recombination technology, and the expression of the Vitreoscilla hemoglobin Vhb gene is improved by carrying out metabolic engineering transformation on the pichia pastoris cell for expression and optimizing the regulation and control of staged fermentation parameters, so that the expression efficiency of the Vitreoscilla hemoglobin Vhb gene is improved. The problems that in existing recombinant protein fermentation, oxygen dependence is high, product degradation is serious, purity is low, and large-scale stability is poor are solved, the yield and purity of recombinant spider silk protein are guaranteed, and large-scale production of the recombinant spider silk protein is facilitated.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

The invention relates to a tryptophan high-yield strain as well as a preparation method and application thereof. The strain comprises overexpression of Vhb protein in an indole synthetic pathway enhanced strain. Heterologous hemoglobin Vhb is introduced into the strain to promote C-H functionalization reaction on the C3 position of indole, and an indole synthesis path is enhanced, so that the yield of tryptophan is increased.

The invention provides multifunctional bionic hydrogel as well as preparation and application thereof in traumatic brain injury treatment, and belongs to the field of biomedical materials. The preparation method comprises the following steps: combining polydopamine modified hemoglobin nanoparticles with curcumin-based carbon quantum dots with anti-inflammatory activity, and embedding into bionic hyaluronic acid-collagen double-network hydrogel, so as to prepare the multifunctional bionic hydrogel. The hydrogel can effectively relieve neuron hypoxia, reduce apoptosis, promote the polarity of microglial cells to be converted into anti-inflammatory phenotype, and significantly down-regulate the expression of S100A8, thereby inhibiting pathological neural immune crosstalk. In a traumatic brain injury model, hydrogel implantation improves the pathological microenvironment, enhances endogenous nerve regeneration, and ultimately results in significant neurological function and cognitive recovery. The multifunctional bionic hydrogel prepared by the invention has a good application prospect in preparation of materials for preventing and / or treating traumatic brain injury.

The invention discloses a hand motion FNIRS signal classification method based on t distribution optimization feature extraction, and the method specifically comprises the steps: employing a portable functional near-infrared collection device to collect an optical density signal of a forehead cortex brain region during the motion execution period during the operation of the device; after pretreatment, the concentration of oxyhemoglobin HBO and the concentration of deoxidized hemoglobin HBR are calculated according to the corrected Beer-Lambert law; according to motion execution and resting state segmentation data, time domain features and time-frequency domain features of oxyhemoglobin concentration data signals of each channel of each experiment test are extracted, and a first feature matrix is formed; performing kernel density estimation and kurtosis and skewness test on the first feature matrix to obtain a second feature matrix, performing t distribution on each corresponding feature satisfying normal distribution in the second feature matrix of the motion execution and resting states, and optimizing feature selection on the basis to obtain an optimized third feature matrix; the optimized third feature matrix is used as classifier input, a brain-computer interface system model training result is obtained, and effective feature selection and extraction are achieved; according to the method, the brain-computer interface system feature extraction of the hand motion FNIRS signals can be optimized, so that the classification performance is improved.

The invention relates to the field of molecular biology, and discloses a nucleic acid releasing agent for isothermal amplification and a use method of the nucleic acid releasing agent. Comprising the following steps: step 1, preparing a polyoxypropylene polyoxyethylene copolymer diluent, a defoaming agent SE-15 diluent, an ethyl phenyl polyethylene glycol NP-40 diluent, a sodium polyethylene sulfonate diluent and a Proclin300 diluent in sequence through RNA enzyme-free water; and 2, sequentially preparing an ethylenediamine tetraacetic acid disodium salt mother solution, a sodium dodecyl sulfate mother solution and a glycine mother solution through RNA enzyme-free water. Sodium polyethylene sulfonate can be specifically combined with protein amplification inhibitors in a sample to remove mucoprotein, hemoglobin, polysaccharide and other inhibitors in the swab sample, and disodium ethylene diamine tetraacetate can chelate metal ions to reduce the influence of nuclease on target nucleic acid.

Disclosed herein are nucleic acid compositions and methods of use. The nucleic acid compositions may have a therapeutic nucleic acid sequence operably linked to a nuclear targeting sequence that increases expression of the therapeutic nucleic acid in a cell by at least 1.25 fold; at least 80% sequence identity to SEQ ID NO: 6; or a first regulatory element comprising a promoter sequence operably linked to a hemoglobin subunit gamma intron (hBGi) sequence, and a second regulatory element comprising a woodchuck hepatitis posttranscriptional regulatory element (WPRE) sequence. The nucleic acid compositions with these features may enhance therapeutic nucleic acid transfection and expression. Also disclosed herein are transgenes optimized for gene therapy applications, including novel FVIII transgene sequences, in which the B domain may be non-naturally occurring the A1 and / or A3 domain may include at least one amino acid substitution.

The invention provides a mobile terminal skin parameter inversion method based on spectral information, and the method comprises the following steps: constructing an optical model according to the characteristics of a skin layered structure and a spectral response mechanism of three skin parameters: melanin content, hemoglobin concentration and dermis thickness, and building a skin parameter and reflection spectrum database based on the model; based on the database, establishing a skin multi-parameter inversion MLP neural network structure adaptive to the collection characteristics of a mobile phone end sensor, and screening an optimal four-waveband channel through multi-waveband combination traversal; the mobile phone spectrum sensor is subjected to wave band optimization, data acquisition and skin parameter inversion. According to the method, through efficient decoupling of a nonlinear coupling relation among multiple skin parameters by waveband optimization and an MLP model, and targeted design of four optimal visible light narrow-band channels, synchronous quantitative detection of melanin, hemoglobin and dermis thickness is realized, core physiological parameters from epidermis to dermis superficial layer are covered, information coverage is comprehensive, and specificity is high.

The invention relates to the technical field of biological detection, in particular to a composition for protein precipitation, a protein precipitation agent containing the composition, a protein precipitation combined product, a detection kit and a preparation method and a detection method of a blood analysis sample. The composition for protein precipitation comprises zinc sulfate and ammonium acetate in a dosage ratio of (5-17) mg: (0.5-1) mmol. Compared with the prior art, the composition has the following beneficial effects that the zinc sulfate and the ammonium acetate are matched according to a specific proportion, so that the specific composition is formed, and hemoglobin in a blood sample can be effectively removed by taking the composition as a protein precipitation component.

The utility model relates to the technical field of livestock and poultry blood utilization, in particular to a hemoglobin low-temperature extraction device which adopts two-stage separation and integrated design, is high in integration level, suitable for small-scale hemoglobin extraction work and good in practicability. Comprising a tank; the storage crushing tank is installed on the upper portion of the tank body, the liquid inlet pipe is installed on the top of the storage crushing tank, the first centrifugal separation tank is installed in the middle of the tank body, the storage crushing tank is communicated with the first centrifugal separation tank through a pipeline, and the second centrifugal separation tank is installed in the middle of the storage crushing tank through a pipeline. A residue discharging pipe is installed on the side wall of the first centrifugal separation tank, the upper end of the conveying pipe is communicated with the first centrifugal separation tank, the second centrifugal separation tank is installed on the lower portion of the tank body, a first liquid discharging pipe is installed on the side wall of the second centrifugal separation tank, and the upper end of the second liquid discharging pipe is communicated with the bottom of a second separation cavity of the second centrifugal separation tank. The upper end of the second liquid discharging pipe is located on the inner side of the inner end of the first liquid discharging pipe.

The application discloses a use of Ejiao Qianggu oral liquid in regulating immunity, and particularly relates to an improving effect of the Ejiao Qianggu oral liquid on an immune system, which is realized by the following aspects. On one hand, the Ejiao Qianggu oral liquid promotes proliferation and transformation (mainly early T lymphocytes and part of B lymphocytes) and active functions of spleen lymphocytes; on the other hand, the Ejiao Qianggu oral liquid improves phagocytosis of macrophages. The Ejiao Qianggu oral liquid has the following characteristics: firstly, the Ejiao Qianggu oral liquid acts on early lymphocyte maturation, and has no significant effect on proliferation of peripheral blood lymphocytes; secondly, the Ejiao Qianggu oral liquid can significantly act on immune target organs, thymus and spleen tissues, and does not excessively stimulate the spleen, unlike positive drugs such as colony stimulating factor which can cause significant edema of the spleen; and thirdly, the Ejiao Qianggu oral liquid has a significant recovery effect on body weight, white blood cells, lymphocytes, red blood cells and hemoglobin in blood of patients with low immunity.

The invention provides a sugar fat disease death risk prediction method and system based on NHANES data, and the method comprises the steps: obtaining the fasting blood glucose FPG and glycosylated hemoglobin HbA1c clinical detection data of a sugar fat disease patient, and calculating an SHR value through a formula; based on the data of the NHANES database, SHR total cause bilateral critical values of total cause death rate and cardiovascular death rate, SHR cardiovascular bilateral critical values and SHR cancer unilateral critical values of cancer death rate are obtained; and comparing the SHR value with an SHR total cause double-side critical value, an SHR cardiovascular double-side critical value and an SHR cancer single-side critical value of the cancer death rate, and carrying out risk layering and evaluation. The biochemical aging acceleration index has a prediction effect on the diabetes death risk, and KDMAgeAccel significantly intermediates the death risk caused by SHR.

The invention provides a protein component composition with special medical application as well as a preparation method and application thereof, and relates to the field of formula food with special medical application. Wherein the protein component composition comprises protein and a sweetening agent; the protein is one or more of hydrolyzed whey protein powder, hydrolyzed casein and albumin peptide. The sweetening agent is one or more of white granulated sugar, glucose syrup, malt syrup, high fructose corn syrup, erythritol, xylitol, maltitol and stevioside. A plurality of components such as a stabilizer, mineral substances and multivitamins can also be added into the protein component composition. The finally obtained composition can be applied to preparation of formula food for special medical purposes, and the content of total protein, hemoglobin, albumin or transferrin in blood of patients suffering from malnutrition or insufficient protein intake is increased.

Disclosed are a composition and method for the diagnosis of diseases accompanied by impaired glucose tolerance, comprising a material for ITIH1 detection. The composition and method according to the present application enable a relatively sensitive response even to small changes in blood sugar according to various stimulants in a hyperglycemic situation as compared to glycated hemoglobin (HbA1c) which is an existing diagnostic marker widely used for patients with diabetes, which is a typical disease accompanied by impaired glucose tolerance, and thus can more precisely detect blood sugar changes under various stress situations.

The present disclosure relates to dihydropyrimidine-2,4(1H,3H)-dione-containing polycyclic compounds, or pharmaceutically acceptable salts thereof, and compositions thereof which are effective to reduce or modulate widely interspaced zinc finger motifs (WIZ) protein expression levels and / or induce fetal hemoglobin (HbF) expression, and also relates to methods of their preparation and their use in treatment of subjects in need.

This invention discloses a method for preparing a core-shell microneedle patch loaded with dual nanoparticles, comprising: preparing silica nanoparticles loaded with oxyhemoglobin and dihydroporphyrin e6; preparing calcium carbonate nanoparticles loaded with aPD-1 antibody; dispersing the silica nanoparticles loaded with oxyhemoglobin and dihydroporphyrin e6 in a polyvinyl alcohol solution to prepare the shell structure of the microneedles; preparing a polymer hydrogel; dispersing the calcium carbonate nanoparticles loaded with aPD-1 antibody in the polymer hydrogel to prepare the core structure of the microneedles; and casting the polyvinyl alcohol solution onto the backing of the microneedles to finally obtain the core-shell microneedle patch loaded with dual nanoparticles. The core-shell microneedle patch loaded with dual nanoparticles prepared by this invention can deliver nanoparticles to the tumor site, where the nanoparticles respond to the tumor microenvironment and activate the body's own immune system, thereby achieving a therapeutic effect on tumors.

The application belongs to the technical field of biological medicine, and particularly relates to a preparation method and application of a styrene microsphere for glycosylated hemoglobin detection. The styrene microsphere is prepared through a dispersion polymerization method, a seed polymerization method and a preparation step of organic high molecular particles. The microsphere prepared in the application has moderate particle size and concentrated distribution, good uniformity of the microsphere particle size, a hydroxyl covering on the surface of the microsphere to form a hydrophilic layer, and can effectively reduce the matrix effect, so as to achieve accurate analysis of glycosylated hemoglobin HbA1c. The result is accurate, stable and small in error in the detection, and the application can be applied to quantitative detection of diabetes.

The invention relates to the technical field of pharmaceutical preparations, in particular to composite condensing gel based on photodynamic therapy and a preparation method of the composite condensing gel. The preparation method of the composite condensate gel based on photodynamic therapy comprises the following steps: preparing a BSA (Bovine Serum Albumin) solution, uniformly mixing an inulin mother solution with the BSA solution, adjusting the pH value, heating in a water bath, cooling to room temperature, adding 5-aminolevulinic acid methyl ester hydrochloride into the condensate gel, adding an acid coagulant, diluting, and filling CO2 gas. According to the invention, CO2 is added into the composite condensation gel, so that the binding force of hemoglobin and O2 in tissues absorbing the composite condensation gel is reduced, and the concentration of free oxygen is improved, so that the composite condensation gel can transfer energy to surrounding oxygen and generate singlet oxygen with strong activity, thereby killing cancer cells and achieving the effect of improving the curative effect of photodynamic therapy.

Glycated Hb was measured in reticulocytes (RNA-positive cells) and was correlated to a decrease in RNA fluorescence intensity in a Log-linear dependent manner, with no statistically significant differences in slope or in the ordinate of the origin between individuals. The loss of RNA fluorescence intensity in reticulocytes observed by flow cytometry is directly related to the time they spend in circulation and consequently to their lifespan. By setting the time that reticulocytes spend to mature in the blood circulation and because the relation between the decrease in log RNA fluorescence intensity and the increase in log HbA1c fluorescence intensity is linear, the inventors are able to calculate the increase in HbA1c fluorescence intensity depending on the time and thus to determine the mean half-life of a RBC population. This new tool is noninvasive and requires only one single time point measurement. The present invention relates to a method for in vitro determination of the age of red blood cells of a set of red blood cells by using intracellular glycated hemoglobin measurement by flow cytometry and by using a standard curve generated from glycated hemoglobin measurement in reticulocyes.

The present application relates to a non-invasive hemoglobin detection method based on hyperspectral image reconstruction, belonging to the field of physiological signal detection. The present application irradiates human skin tissue by using a visible light source, simultaneously uses a hyperspectral camera and an RGB camera to simultaneously collect images of the irradiated skin area as a training set, trains a deep learning model, and realizes hyperspectral reconstruction of ordinary RGB images. A hemoglobin extraction feature extraction model for the visible light range diffuse reflectance spectrum is established using an algorithm, and the real hemoglobin content of the measured human body is output through a hemoglobin correction model.

A recombinant hemoglobin, a nucleic acid encoding the recombinant hemoglobin, and a preparation method therefor are provided to address the current problems of complex processes, high production costs, and low yields that exist for improving a hemoglobin having a stable tetrameric structure. The recombinant hemoglobin comprises: two or more units of recombinant hemoglobins, the units of recombinant hemoglobins each comprise four polypeptide chains, and the polypeptide chains are two α chains and two β chains. The amino acid sequences of the four polypeptide chains of each unit of recombinant hemoglobin are linked by means of intra-unit linker peptides to form the unit of recombinant hemoglobin, and two or more of the units are linked by means of inter-unit linker peptides.

The invention relates to the technical field of pickling of meat products, and discloses an internal and external coloring pickling process of meat products, which comprises the following steps: firstly collecting blood of a meat product to be pickled, separating blood cells from plasma by adopting a centrifugal separation method, and then carrying out hemolysis treatment on the blood cells by utilizing an acidic buffer solution. According to the internal and external coloring and pickling process of the meat product, processing equipment, a natural coloring agent, a natural preservative and a natural pickling agent are adopted, and when hemoglobin pigment is extracted, centrifugal separation and ion exchange chromatography technologies are applied, so that high purity of the pigment is ensured, and a basis is provided for stable internal coloring. In the rolling and pickling link, composite natural microorganism fermentation pickling liquid is used, components of the composite natural microorganism fermentation pickling liquid are carefully screened and cultured, microorganisms such as lactic acid bacteria and the like and metabolite antibacterial polypeptides thereof not only can inhibit growth and reproduction of harmful microorganisms, but also can enable meat products to uniformly absorb components such as salt and spices in the pickling process, so that the meat products are not prone to damage. And the quality difference caused by non-uniform pickling is effectively avoided.

The invention relates to a glycosylated hemoglobin detection method, a glycosylated hemoglobin detection device and electronic equipment, and relates to the technical field of medical detection, the method in one embodiment comprises the following steps: obtaining a chromatogram signal obtained by performing signal detection on a sample, and determining a plurality of candidate peaks based on a local maximum value in the chromatogram signal; identifying a peak of glycosylated hemoglobin and a peak of a hemoglobin variant from the plurality of candidate peaks; determining the initial content of the glycosylated hemoglobin based on the first peak area of the peak of the glycosylated hemoglobin and the total area of the candidate peaks; determining the content of the hemoglobin variant based on the second peak area of the peak of the hemoglobin variant and the total area of the candidate peaks; and correcting the initial content of the glycosylated hemoglobin based on the content of the hemoglobin variant to obtain the corrected content of the glycosylated hemoglobin. By adopting the scheme of the embodiment, the detection accuracy of the glycosylated hemoglobin is improved.

The invention relates to a glycosylated hemoglobin HbA1c freeze-drying detection reagent and a preparation method and application thereof.The preparation method comprises the steps that surface aminated polystyrene microspheres are prepared through an emulsion polymerization method, then double bonds are introduced through Schiff base reaction and methylacrolein reaction, and the glycosylated hemoglobin HbA1c freeze-drying detection reagent is obtained. Then preparing anionic polystyrene microspheres with hydrophilic chains through free radical polymerization, and preparing freeze-dried powder of the HbA1c detection reagent R1 by adopting the anionic polystyrene microspheres; the preparation method comprises the following steps: preparing a hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde, then carrying out a Schiff base reaction on the hydrophilic cross-linking agent 2, 5-bis (2, 4-dihydroxy-6-formylphenoxy) terephthalaldehyde and HbA1c mAb to prepare an HbA1c oligomer, and preparing the HbA1c detection reagent R2 freeze-dried powder by using the HbA1c oligomer. The glycosylated hemoglobin HbA1c freeze-drying detection reagent prepared by the invention has the advantages of high antigen binding efficiency, excellent detection sensitivity, sufficient retention of antibody activity and strong detection specificity.