475 results about "Hemoglobin hb" patented technology

The invention relates to the technical field of intraoperative bleeding amount monitoring, and provides an intelligent intraoperative bleeding amount monitoring and alarming method and system.The method comprises the steps that during an operation, blood, body fluid and cleaning fluid in an operation area are cleaned in time through a suction bottle and gauze; after the gauze is used, the absorbed liquid is partially screwed out and flows into the liquid collecting tank; weight changes of the suction bottle and the gauze storage area are detected in real time respectively, signals are transmitted to the data processing module, the concentration of hemoglobin in the two kinds of liquid is detected, the concentration of blood in the liquid is calculated, and the concentration of the hemoglobin in the liquid is calculated by combining the amount of the liquid sucked by the suction bottle and the amount of the liquid taken out by the gauze in real time. The amount of blood brought out by the gauze and the amount of blood sucked out by the suction bottle are calculated in real time, the real-time bleeding amount of a patient in an operation is obtained, and when the real-time bleeding amount reaches a threshold value, early warning is given out. The problems that errors are large in a traditional method, and particularly in a large amount of blood loss or a complex operation scene, the blood loss dynamic state is difficult to master timely and accurately are solved.

The invention provides an intense pulsed light skin beauty local area identification method and system, and relates to the technical field of phototherapy, and the method comprises the steps: collecting an original face image of a patient, obtaining a historical skin treatment segmentation data set, carrying out the preprocessing of the original face image, and obtaining a standard face image; based on image feature information in the skin treatment segmentation data set, constructing a skin region division model, inputting the facial standard image into the skin region division model for target recognition, and obtaining a plurality of to-be-treated region images in the facial standard image; performing independent component analysis on the to-be-treated area image to obtain a melanin channel and a hemoglobin channel in the to-be-treated area image; constructing a multi-modal attention network model, inputting the melanin channel and the hemoglobin channel into the multi-modal attention network model for feature extraction and segmentation, and respectively obtaining melanin segmentation information and hemoglobin segmentation information; and fusing the melanin segmentation information and the hemoglobin segmentation information to obtain a skin treatment segmentation map, collecting treatment features in the skin treatment segmentation map, classifying the treatment features according to a random forest classifier, obtaining a skin treatment type corresponding to each to-be-treated area image, and obtaining the skin treatment type corresponding to each to-be-treated area image. And a treatment scheme corresponding to the skin treatment type is given. According to the method, the local detail sensitivity and the global information processing efficiency of the skin region division model can be improved.

The invention relates to a recombinant pichia pastoris strain capable of producing astaxanthin at high yield as well as a construction method and application of the recombinant pichia pastoris strain. The recombinant pichia pastoris strain is obtained by expressing ATP (adenosine triphosphate) citrate lyase (ACL) and acetyl-CoA synthase (ACS) or phosphoketolase (PK) and phosphotransacetylase (PTA), choline kinase (CK), inositol polyphosphate kinase (IPK) and vitreoscilla hemoglobin (VHB) in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified strain PP-LC2. The astaxanthin production performance of the recombinant strain is verified on the basis of comparison of precursor supply pathways, IUP pathway construction and improvement of the oxygen supply capacity of the engineering strain, and the astaxanthin production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of astaxanthin can be better promoted, the engineering strain can efficiently synthesize the astaxanthin by utilizing methanol through amplification fermentation of a 5L fermentation tank, and industrial production is facilitated.

The invention belongs to the technical field of microorganisms, and provides streptococcus salivarius subsp. Thermophilus HX-ST36, and the preservation number of the streptococcus salivarius subsp. Thermophilus HX-ST36 is CGMCC NO.32955. The streptococcus salivarius subsp. Thermophilus provided by the invention can be used for fermenting astragalus membranaceus-angelica sinensis. In a cold coagulation and blood stasis type primary dysmenorrheal model mouse, a fermentation product can remarkably inhibit the writhing reaction, and the effects of relieving dysmenorrheal and enriching blood can be achieved by reducing the levels of prostaglandin PGF2 and vasopressin ADH in uterine tissue and increasing the content of hemoglobin (HGB). The dampness clearing effect of the fermentation product is verified by improving the heavy physical sign of moisture through crowd experiments. The invention also provides a preparation method of the probiotic preparation and the probiotic fermentation product. The preparation methods of the probiotic preparation and the probiotic fermentation product are simple, and large-scale production can be realized.

The invention discloses a traditional Chinese medicine composition with the effects of benefiting qi, nourishing blood, invigorating the spleen and tonifying the kidney and a preparation method of the traditional Chinese medicine composition. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: cistanche, ginseng, American ginseng, astragalus membranaceus, medlar, rhizoma polygonati, oyster peptide, angelica sinensis, radix rehmanniae preparata, donkey-hide gelatin, fructus amomi, pericarpium citri reticulatae, hawthorn, folium cortex eucommiae, radix puerariae, liquorice and the like. The preparation method comprises the following steps: respectively crushing the medicinal materials, dissolving the colla corii asini, mixing, adding water, granulating and the like. The composition can be prepared into various dosage forms such as granules, capsules, tablets, oral liquid and the like. Experiments show that the traditional Chinese medicine composition can effectively improve the symptoms of deficiency of qi and blood (increasing erythrocyte count and hemoglobin content), weakness of spleen and stomach (increasing body weight and improving pepsin activity) and kidney deficiency (reducing creatinine and urea nitrogen content and improving SOD activity), and acute toxicity experiments show that the toxicity is slightly reversible under high dosage, and the safety is good under therapeutic dosage. The traditional Chinese medicine composition has potential application value in the aspect of preparing medicines for benefiting qi, nourishing blood, invigorating spleen and tonifying kidney.

According to the application of the ZIF-8 coated hemoglobin composite material in preparation of the lithium-sulfur battery, an enzyme solution, an organic ligand and metal ions are co-precipitated through in-situ biomineralization to prepare an MOF-enzyme compound, the method is not influenced by the size of enzyme molecules, the synthesis condition of ZIF-8 is mild, the structure of enzyme cannot be damaged in the reaction process, and the preparation method is simple and easy to implement. And the synthesis process has the advantages of short reaction time, no need of other organic solvents, capability of expanding multiple synthesis, simple synthesis method and high yield. The results of XRD, FIIR and XPS experiments show that the MOF (at) enzyme compound prepared by the method preserves the inherent structure of the enzyme, and when the MOF (at) enzyme compound is assembled into a battery, the electrochemical performance and the dynamic performance of the battery containing ZIF-8 (at) Hb are better.

The invention relates to the technical field of bleeding amount monitoring, in particular to a hysteroscopic surgery bleeding amount monitoring method, which comprises the following steps of: performing preprocessing and feature extraction on an acquired hysteroscopic image, and determining a bleeding point location and a bleeding area; establishing a bleeding model, and dynamically calculating a first value of the bleeding amount according to the bleeding point location and the bleeding area data; a multispectral sensor is arranged in the suction pipeline, liquid components are analyzed in real time through multiple wavelengths, and the hemoglobin concentration is calculated; determining a second value of the amount of bleeding based on the fluid flow rate within the aspiration conduit; collecting heart rate and blood pressure data of a patient, performing data fusion in combination with the first value and the second value of the bleeding amount, dynamically adjusting the weight, and outputting an estimated value of the bleeding amount; setting graded early warning thresholds, and executing prompts of corresponding grades when the thresholds are reached; according to the invention, dynamic weight adjustment is carried out on the first value and the second value; and combining the first value and the second value with the dynamic weight, fusing and outputting a bleeding amount prediction value so as to improve the prediction accuracy.

A glucose monitoring system comprising a sensor control device comprising an analyte sensor coupled with sensor electronics, the sensor control device configured to transmit data indicative of an analyte level of a subject, and a reader device. The reader device comprises a wireless communication circuitry configured to receive the data indicative of the analyte level and a glycated hemoglobin level for the subject, a non-transitory memory, and at least one processor communicatively coupled to the non-transitory memory and the analyte sensor and configured: calculate a plurality of personalized glucose metrics for the subject using at least one physiological parameter and at least one of the received data indicative of the analyte level or the received glycated hemoglobin level, and a display, on a display of the reader device, a report comprising a plurality of interfaces including at least two or more of the received data indicative of the analyte level, the received glycated hemoglobin level, or the calculated plurality of personalized glucose metrics, wherein the plurality of interfaces comprising the report are based on a user type.

The invention provides an oxygen-carrying protein molecule and a preparation method and application thereof, and relates to the technical field of functional protein preparation, the oxygen-carrying protein molecule comprises at least one cysteine part and at least one histidine part, the cysteine part comprises acetyl or ethyl maleoyl, and the histidine part comprises histidine. The histidine moiety includes a benzimidazole derivative group. The benzimidazole derivative is adopted as an auxiliary molecule and participates in the modification process of the sulfydryl modified compound on the hemoglobin, the modification site selectivity is improved, the conformational change is reduced, and the modification degree is accurately controlled, so that the oxygen binding force P50 of the modified hemoglobin is effectively improved.

The invention discloses a wound healing state evaluation method and system based on artificial intelligence, and belongs to the field of wound healing evaluation. The method comprises the following steps: integrating medical record data and wound physiological parameters, and extracting relevance between a medication history and an infection type to form a multi-dimensional pathological factor; hemoglobin oxygenation, blood flow and metabolic heat are subjected to cross-period correlation analysis to construct dynamic physiological time-space information, pathological factors and the physiological time-space information are fused into a heterogeneous graph, nodes comprise drug intensity quantized values, infection risk levels and microcirculation fluctuation values, and edge weights are determined by the time sequence matching degree of drug doses and blood flow changes; based on the node weight and the edge association relationship, the treatment time and the physiological parameter acquisition period are aligned to generate a spatio-temporal evolution matrix, the spatial distribution of the infection risk level and the metabolic heat value characteristics are combined, the dynamic association of the drug action intensity and the microcirculation fluctuation is analyzed, and wound healing evaluation information is generated. The accuracy of wound healing state evaluation can be improved.

The invention discloses a non-invasive wearable vascular arteriosclerosis assessment system and method based on multi-mode pulse wave signal fusion, and belongs to the field of vascular function assessment. The system comprises a distributed acquisition front end, a butterfly-shaped flexible substrate, an integrated double-accelerometer, a four-wavelength LED (530nm / 660nm superficial blood vessel measurement), a 810nm / 940nm deep artery measurement device, a double-photodiode, a double-impedance electrode and a pressure sensor. The central integrated control end processes a multi-mode signal and realizes pressure-LED current closed-loop control; the upper computer terminal extracts the multi-modal pulse wave time difference of the limbs and the neck, the local and regional pulse wave conduction velocity and the blood oxygen hemoglobin change parameters induced by the standardized posture, the dimension is reduced to 30 through principal component analysis, then the parameters are input into the random forest regression model, and the vascular elasticity index is output. Signal stability is improved through flexible fitting, single-source limitation is solved through multi-modal fusion, signal quality is guaranteed through closed-loop control, sensitivity is enhanced through dynamic physiological excitation, and accuracy and universality of arteriosclerosis assessment are improved.

A method for recombinantly expressing feline serum albumin. A method for constructing an engineered strain for recombinantly expressing a feline serum albumin, wherein the method comprises: co-expressing a feline serum albumin, a protein disulfide isomerase, and regulatory factor HAC1 and / or vitreoscilla hemoglobin in a receptor yeast. The obtained strain is an engineered strain capable of expressing a recombinant feline serum albumin. The engineered strain can efficiently express rFSA. Compared with the direct and individual expression of rFSA, the expression level of rFSA in the co-expression strain is significantly increased, and the highest expression level can reach about 16 g / L. This lays a foundation for large-scale industrial production of rFSA.

The invention discloses a focused ultrasound transcranial nerve regulation and control platform and method based on photoacoustic imaging. The system comprises a synchronous pulse generator, a pulse ultrasound transmitting system, a photoacoustic signal collecting system and a fixing device. According to the method, the limitation of other imaging monitoring modes is overcome, monitoring is carried out by utilizing the characteristics of non-invasiveness, high resolution and high contrast of photoacoustic imaging, the TUS progress can be monitored for providing real-time dynamic imaging, and functional information can be provided; photoacoustic imaging is applied to focused ultrasound transcranial nerve regulation for the first time. And meanwhile, by utilizing high sound resolution and high optical contrast of photoacoustic imaging, the strongest photoacoustic signal can be provided in a near-infrared region for hemoglobin of a seat endogenous contrast agent, so that the PAT becomes an ideal imaging tool for detecting cerebral hemodynamics. In addition, key pathophysiological parameters are evaluated through functional imaging provided by the PAT, and the method can be used for evaluating brain changes in the low-intensity focused ultrasound stimulation process.

The invention discloses an endoscope image processing method based on oxyhemoglobin saturation and multiple central wavelengths, and relates to the field of medical image processing.The method comprises the steps that a first initial image signal, a second initial image signal, a third initial image signal and a fourth initial image signal are obtained; calculating oxyhemoglobin concentration information of the target part according to the second initial image signal and the third initial image signal; calculating deoxidized hemoglobin concentration information of the target part according to the second initial image signal and the fourth initial image signal; calculating the oxyhemoglobin saturation of the target part according to the oxyhemoglobin concentration information and the deoxidized hemoglobin concentration information; performing brightness enhancement on the second initial image signal based on the oxyhemoglobin saturation to obtain a second enhanced image signal; and generating a target image according to the second enhanced image signal, the first initial image signal and the third initial image signal, wherein the target image is used for identifying the bleeding position of the target part.

The invention relates to a cognitive state classification method based on EEG-fNIRS spatio-temporal fusion features, which comprises the following steps of: setting an experiment according to a mental calculation experiment normal form, and synchronously acquiring EEG data and fNIRS data of a tested mental calculation task; preprocessing the two collected data to obtain electroencephalogram signal data and hemoglobin concentration change data; eEG signal data and hemoglobin concentration change data are taken, data enhancement is carried out through a time window, then the data are sent to a space-time fusion network, EEG features, oxyhemoglobin HBO features and deoxyhemoglobin HBR features are obtained, fusion features are obtained through fusion, and an EEG classification result, an fNIRS classification result and a fusion feature classification result are obtained. According to the method, the characteristics of the EEG signal and the fNIRS signal are effectively combined, the overfitting problem in modal deep learning is relieved, and cognitive state classification can be accurately carried out.

The invention discloses an intelligent monitoring device for chest drainage components, which comprises a spectrum analyzer and a drainage bin, a main control chip; an output unit; a power supply unit; a detection area is arranged on one side of the spectrum analyzer, a detection end capable of stretching into the detection area is arranged on one side of the drainage bin, a detection cavity allowing drainage liquid to enter is formed in the detection end, the optical detection module comprises a light source module capable of emitting light beams to the detection cavity and a detector used for receiving reflected light beams, and the detector is connected with the main control chip. A reflective optical detection technology is adopted, monitoring of key components such as hemoglobin, chylomicroparticles and inflammatory factors is realized at the same time, a characteristic absorption spectrum of a specific biomarker can be covered by a multi-wavelength light source module, and concentration change trends of various components are synchronously obtained by combining absorbance dynamic analysis of a main control chip; the limitation of single parameter detection of a traditional device is broken through.

The invention belongs to the technical field of medical treatment, and particularly relates to a hemoglobin chyle joint detection device and a detection method.The hemoglobin chyle joint detection device comprises a shell, an analysis mechanism is arranged in the shell, and a colorimetric tube is inserted into the upper surface of the shell; the analysis mechanism comprises a light source module, a sample processing module, an optical detection module and a data processing module; the optical detection module synchronously acquires absorbance signals under two wavelengths through double detectors; the data processing module calculates hemoglobin concentration and chyle degree based on the dual wavelength absorbance values. The method is different from traditional single-index detection, two-parameter synchronous analysis of hemoglobin and chyle is achieved for the first time, and the preliminary screening efficiency before blood donation is remarkably improved. By adopting a dual-wavelength correction algorithm, the influence of residual absorption interference of hemoglobin at 580-700nm on chyle index determination is eliminated, and the influence of absorption interference of chyle at 480-570nm on hemoglobin determination is eliminated. The method is suitable for rapid screening on a blood sampling site to realize informatization management of detection data.

The invention discloses a method for biological high-density synthesis of retinaldehyde and a synthetic strain thereof. The synthetic strain is obtained by expressing ATP citrate lyase ACL, acetyl-CoA synthase ACS, choline kinase CK, inositol polyphosphate kinase IPK and vitreoscilla hemoglobin VHb in host bacteria. Wherein the host bacterium is a pichia pastoris gene modified bacterial strain PP-B2C. The retinaldehyde production performance of the recombinant strain is verified on the basis of a precursor supply pathway, an IUP pathway and a dissolved oxygen level, and the retinaldehyde production capacity of the pichia pastoris is further improved. The construction method of the recombinant pichia pastoris is simple, the synthesis of retinaldehyde can be better promoted, and the retinaldehyde can be efficiently synthesized by the engineering strain by utilizing glucose and methanol through amplification fermentation of a 5L fermentation tank, so that the recombinant pichia pastoris is beneficial to industrial production.

The invention relates to the technical field of filler preparation, and discloses an analysis filler for separating glycosylated hemoglobin and a preparation method thereof.The analysis filler for separating glycosylated hemoglobin is obtained by synthesizing nonporous Polymer microspheres of 5 micrometers through a dispersion polymerization method, conducting hydrophilic treatment on the surfaces of the Polymer microspheres to obtain Polymer base balls and finally introducing ion exchange groups to the Polymer base balls. The hydrophilicity of the surface of the matrix is enhanced through hydrophilization treatment, non-specific adsorption is reduced, meanwhile, the swelling property of the filler in water is not affected, and the mechanical strength is not affected. The glycosylated hemoglobin analysis filler which is uniform in granularity, good in separation effect and long in service life can be obtained through the preparation method of the analysis filler.

The invention belongs to the technical field of cell engineering and biochemical detection, and particularly relates to an erythrocyte membrane with a complete structure and a preparation method and application thereof. The red blood cells are split by limiting the components of the release liquid, the conditions are mild, protein components such as hemoglobin in the red blood cells can be released, the cell attributes are removed, the integrity of the internal skeleton structure and the activity of the membrane surface antigen are reserved, the complete structure of the red cell membrane is kept, meanwhile, the key blood group antigen on the surface is reserved, and the effect is good. The problem of antigenicity reduction caused by erythrocyte membrane fragmentation is avoided. After the erythrocytes are lysed, the nanoparticles are coupled to the surface of the erythrocyte membrane, so that the agglutination reaction of the erythrocyte membrane under the induction of the antibody can be visualized. The engineering red blood cell biological membrane is preserved by adopting a fixed program, so that the damage of ice crystals to the engineering red blood cell biological membrane can be effectively avoided, the antigenicity of the membrane is not influenced, the membrane can be preserved for a long time at normal temperature, and the shelf life can be prolonged to two years or more.

Provided herein are red blood cell control compositions comprising one or more populations of lysable hydrogel particles having an impedance that is substantially similar to the impedance of a human red blood cell of average diameter; and a population of hemoglobin molecules or a population of dye molecules that have substantially similar absorbance as hemoglobin; wherein (i) and / or (ii) are present in an amount that corresponds to a normal blood sample or disease state, or (i) and (ii) are present at a ratio that corresponds to a normal blood sample or disease state.

The invention belongs to the technical field of biology, and particularly discloses a high-density fermentation method and application of recombinant spider silk protein, and the high-density fermentation method comprises the following steps: firstly, transforming an expression vector carrying spider silk protein genes into modified pichia pastoris cells to obtain a strain; the activated strain is subjected to expanding culture, high-density fermentation culture and purification, and recombinant spider silk protein is obtained; wherein the transformation means of the pichia pastoris cell is to integrate Vitreoscilla hemoglobin Vhb gene into the pichia pastoris cell by utilizing a gene recombination technology, and the expression of the Vitreoscilla hemoglobin Vhb gene is improved by carrying out metabolic engineering transformation on the pichia pastoris cell for expression and optimizing the regulation and control of staged fermentation parameters, so that the expression efficiency of the Vitreoscilla hemoglobin Vhb gene is improved. The problems that in existing recombinant protein fermentation, oxygen dependence is high, product degradation is serious, purity is low, and large-scale stability is poor are solved, the yield and purity of recombinant spider silk protein are guaranteed, and large-scale production of the recombinant spider silk protein is facilitated.

The invention relates to the technical field of bioengineering, in particular to an SOD (superoxide dismutase) impurity removal and purification process which comprises the following steps: extracting SOD enzyme from fresh animal blood, separating high-purity red blood cells through gradient centrifugation, preparing a crude extract by combining hypotonic lysis and freeze thawing strengthening, then selectively precipitating hemoglobin and other heat-sensitive impurity proteins by adopting a gradient temperature rise thermal denaturation method, and purifying to obtain the SOD impurity removal and purification process. The method comprises the following steps: extracting SOD, removing pigments and lipid impurities by using a mixed adsorbent, further enriching SOD by using ammonium sulfate salting-out, and finally obtaining high-purity SOD powder through vacuum freeze drying by combining ion exchange chromatography and ultrafiltration concentration technologies. The method has the advantages of high efficiency, rapidness and low cost, impurities can be effectively removed through a combined process of hypotonic / freeze-thaw cracking, hemoglobin removal through thermal denaturation, ammonium sulfate salting-out, ion exchange chromatography and ultrafiltration concentration, the purity and the recovery rate of the SOD enzyme are remarkably improved, the whole purification process is carried out at 4 DEG C, and the method is suitable for industrial production. The influence of high temperature on the activity of the SOD enzyme is avoided.

The invention discloses a Yarrowia lipolytica engineering bacterium for producing astaxanthin in a peroxisome compartment and application of the Yarrowia lipolytica engineering bacterium. A complete astaxanthin synthesis path is expressed in host bacteria by recombinant Yarrowia lipolytica; comprising geranyl geranyl diphosphate synthase CrtE, phytoene synthetase / lycopene cyclase CrtYB, phytoene desaturase CrtI, 3-hydroxy-3-methylglutaryl CoA reductase tHMGR, beta-carotene ketolase CrtW and beta-carotene hydroxylase CrtZ, and the enzymes are relocated into peroxisome, so that the enzyme activity of the peroxisome is improved, and the enzyme activity of the peroxisome is improved. The number of expression hemoglobin genes VHb and peroxisome is increased, the surface area of the hemoglobin genes VHb is enlarged, and one or more of enzymes PEX11, PEX19, DNM1 or PHO85 for prolonging the service life of the hemoglobin genes VHb and peroxisome is / are obtained; the astaxanthin production performance of the recombinant strain is verified based on cytoplasm engineering and subcellular organelle compartment engineering, and the astaxanthin production capacity of the yarrowia lipolytica is further improved.

Mean corpuscular parameter values for a sample, such as a mean corpuscular hemoglobin or mean corpuscular volume for the red blood cells in a sample, can be calculated based on individual cell corpuscular parameter values for the red blood cells in the sample. These values may be obtained using a machine learning algorithm. Such a machine learning algorithm may be trained using sets of training images annotated with transducer derived mean corpuscular parameter values, thereby allowing a flow imaging based analyzer to provide parameter values which may otherwise not be available with that type of analysis tool.

The invention relates to a tryptophan high-yield strain as well as a preparation method and application thereof. The strain comprises overexpression of Vhb protein in an indole synthetic pathway enhanced strain. Heterologous hemoglobin Vhb is introduced into the strain to promote C-H functionalization reaction on the C3 position of indole, and an indole synthesis path is enhanced, so that the yield of tryptophan is increased.

The invention provides a folic acid quality control product prepared by using an alternative matrix, and belongs to the technical field of medical detection. The folic acid quality control product is prepared from 5-methyltetrahydrofolic acid, 5, 10-methylenetetrahydrofolic acid and a substitute matrix, the alternative matrix comprises bovine hemoglobin, L-ascorbic acid and a glycine-hydrochloric acid buffer solution (pH 3.0). The alternative matrix can meet the matrix effect requirement and can stabilize the 5-methyltetrahydrofolic acid and the 5, 10-methylenetetrahydrofolic acid for a long time, and the matrix components can be obtained from commercial ways and are low in cost, so that the folic acid quality control product provided by the invention can be widely applied to production and use of folic acid detection reagents or kits, and has wide application prospects. The folic acid detection kit has important significance on market popularization and application of the folic acid detection kit.

The invention provides multifunctional bionic hydrogel as well as preparation and application thereof in traumatic brain injury treatment, and belongs to the field of biomedical materials. The preparation method comprises the following steps: combining polydopamine modified hemoglobin nanoparticles with curcumin-based carbon quantum dots with anti-inflammatory activity, and embedding into bionic hyaluronic acid-collagen double-network hydrogel, so as to prepare the multifunctional bionic hydrogel. The hydrogel can effectively relieve neuron hypoxia, reduce apoptosis, promote the polarity of microglial cells to be converted into anti-inflammatory phenotype, and significantly down-regulate the expression of S100A8, thereby inhibiting pathological neural immune crosstalk. In a traumatic brain injury model, hydrogel implantation improves the pathological microenvironment, enhances endogenous nerve regeneration, and ultimately results in significant neurological function and cognitive recovery. The multifunctional bionic hydrogel prepared by the invention has a good application prospect in preparation of materials for preventing and / or treating traumatic brain injury.

The invention discloses a hand motion FNIRS signal classification method based on t distribution optimization feature extraction, and the method specifically comprises the steps: employing a portable functional near-infrared collection device to collect an optical density signal of a forehead cortex brain region during the motion execution period during the operation of the device; after pretreatment, the concentration of oxyhemoglobin HBO and the concentration of deoxidized hemoglobin HBR are calculated according to the corrected Beer-Lambert law; according to motion execution and resting state segmentation data, time domain features and time-frequency domain features of oxyhemoglobin concentration data signals of each channel of each experiment test are extracted, and a first feature matrix is formed; performing kernel density estimation and kurtosis and skewness test on the first feature matrix to obtain a second feature matrix, performing t distribution on each corresponding feature satisfying normal distribution in the second feature matrix of the motion execution and resting states, and optimizing feature selection on the basis to obtain an optimized third feature matrix; the optimized third feature matrix is used as classifier input, a brain-computer interface system model training result is obtained, and effective feature selection and extraction are achieved; according to the method, the brain-computer interface system feature extraction of the hand motion FNIRS signals can be optimized, so that the classification performance is improved.

The invention relates to the field of molecular biology, and discloses a nucleic acid releasing agent for isothermal amplification and a use method of the nucleic acid releasing agent. Comprising the following steps: step 1, preparing a polyoxypropylene polyoxyethylene copolymer diluent, a defoaming agent SE-15 diluent, an ethyl phenyl polyethylene glycol NP-40 diluent, a sodium polyethylene sulfonate diluent and a Proclin300 diluent in sequence through RNA enzyme-free water; and 2, sequentially preparing an ethylenediamine tetraacetic acid disodium salt mother solution, a sodium dodecyl sulfate mother solution and a glycine mother solution through RNA enzyme-free water. Sodium polyethylene sulfonate can be specifically combined with protein amplification inhibitors in a sample to remove mucoprotein, hemoglobin, polysaccharide and other inhibitors in the swab sample, and disodium ethylene diamine tetraacetate can chelate metal ions to reduce the influence of nuclease on target nucleic acid.