463 results about "Hemoglobin hb" patented technology

Methods and apparatus for measuring cerebral O₂ saturation and detecting cerebral hypoxia-ischemia using multi-wavelength near infrared spectroscopy (NIRS). Near-infrared light produced by an emitter is directed through brain tissue. The intensity of the light that passes through the brain tissue is measured using photodiode detectors positioned at distinct distances from the emitter. This process is conducted for at least three wavelengths of near-infrared light. One of the wavelengths used is substantially at an isobestic point for oxy-hemoglobin and deoxy-hemoglobin, but the other two may be any wavelengths within the near-infrared spectrum (700 nm to 900 nm), so long as one of the additional wavelengths is greater than the isobestic point and the other is less than the isobestic point. Tissue oxygenation is calculated using an algorithm derived from the Beer-Lambert law. Cerebral hypoxia-ischemia may be diagnosed using the calculated tissue oxygenation value.

In certain aspects, the present invention provides compositions and methods for increasing red blood cell and/or hemoglobin levels in vertebrates, including rodents and primates, and particularly in humans.

The present invention relates to a testing system for assessing hypoxia induced cellular damage in a mammal including human, comprising a disposable device having a sample inlet and a collection chamber separated by a separation device wherein the collection chamber is connected to at least two, a first and a second, visible detection compartments, whereof at least one is arranged with chemical means for direct visual detection, said first detection compartment being arranged to determine whether level of hemoglobin (Hb) in a sample of body fluid taken from said mammal exceeds a predetermined threshold value, and said second detection compartment being arranged to evaluate level of total amount of lactate dehydrogenase (LDH) in said sample.

The present invention relates to methods arid compositions for isolating hemoglobin from red blood cells. Such methods and compositions also facilitate viral inactivation in a manner that allows recovery of biologically active hemoglobin. More particularly, this method relates to the use of solvents and detergents that are capable of facilitating red blood cell lysis to release hemoglobin (and solubilizing red blood cell membranes), while simultaneously inactivating viruses.

The present invention discloses a high-yield gamma-polyglutamic acid production gene engineering bacterial and a fermentation production method thereof. The gene engineering bacterial is named Bacillus subtilis FRD518, wherein the preservation number is CGMCC NO.6772, and the Vitreoscilla hemoglobin gene (vgb) is recombined and integrated on the chromosome of the gene engineering bacterial, such that the Vitreoscilla hemoglobin VHb can be successfully and highly expressed so as to significantly improve the oxygen utilization rate of the recombinant Bacillus subtilis under a low dissolved oxygen condition. According to the present invention, during a fermentation process, a carbon source, sodium glutamate, a yeast extract and other components are added in a flow manner, such that the gene engineering bacterial can efficiently produce the gamma-polyglutamic acid in a high yield manner, wherein the yield achieves more than 65 g/L, and is increased by 147% compared with the yield of the single batch culture of the original wild strain; and with the gene engineering bacterial, the problems of low yield, more by-products, long period, high energy consumption and the like of fermentation of the gamma-polyglutamic acid gene engineering bacterial under high viscosity and dissolved oxygen limiting conditions are solved, and the gamma-polyglutamic acid gene engineering bacterial can be applied for large-scale industrial production of gamma-polyglutamate acid.

The present invention provides a hemoglobin molecule (Hb) having six±one PEG chains, wherein two of said PEG chains are bound to Cys-93 (β) of Hb, and the remaining PEG chains are bound to thiol groups introduced on ε-NH₂ of Hb. The present invention also provides a process for preparing a modified hemoglobin molecule (Hb), comprising the steps of: (a) reacting Hb with 8-15 fold excess of iminothiolane to form thiolated Hb; and (b) reacting the thiolated Hb with 16-30 fold excess of PEG functionalized with a maleimide moiety, to form the modified Hb.

The invention discloses an embedded non-invasive detection system of human body physiological information and a data processing method. The system comprises a detector housing and a detection circuit board and a detection groove which are installed inside the detector housing; infrared diode arrays are installed at two sides of the detection groove; an inclined bench is installed at the bottom of the detection groove; an inclined optical filter is installed above the inclined bench and the inclined optical filter is paralleled with an upper surface of the inclined bench; a cavity is installed between the inclined optical filter and the inclined bench, wherein the cavity is provided with an image acquisition module and an photoelectric volume pulse detection circuit therein; a connection position of the detection groove and an inclined bottom of the inclined optical filter is provided with a finger tip cavity which is provided with a finger switch therein. The system of the invention is convenient to use and is dustproof; human body information such as heart rate, blood oxygen and hemoglobin concentration can be continuously, noninvasively and in real time monitored; at the same time, vein images of users can be conveniently and quickly acquired so as to realize efficient and accurate noninvasive detection; the system and method of the invention can be widely applied in health check-up for hospitals.

A method for performing a photopheresis procedure is provided comprising collecting MNCs in a suspension comprising RBCs and plasma and lysing the red blood cells in the solution, preferably by combining the suspension with a solution to cause lysis. In one example, the solution for causing lysis of the red blood cells comprises ammonium chloride, and the suspension including the ammonium chloride is incubated to cause lysing. After lysing, the suspension may be washed to remove plasma and hemoglobin freed by the lysis of the red blood cells, and an ultraviolet light activated substance is added to the suspension. The suspension is then irradiated with ultraviolet light.

The invention discloses a functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode as well as a preparation method and an application thereof. According to the technical scheme provided by the invention, firstly the fixation of an ionic liquid-hydrotalcite-like composite material to hemoglobin is realized by utilizing a coprecipitation method, then obtained hemoglobin-functionalized ionic liquid-hydrotalcite-like composite material is prepared into a solution which is dropwise coated on the surface of a preprocessed glassy carbon electrode, and the functionalized ionic liquid-hydrotalcite-like composite material fixed protein modified electrode is obtained after natural drying. According to the obtained composite modified electrode, the superiorities of functionalized ionic liquid and layered inorganic materials in the aspect of electrode modified materials are adequately combined, so that the modified electrode has high electrical conductivity and good dispersibility and enzyme fixation capacity. A hydrogen peroxide electrochemical sensor based on the modified electrode has the advantages of simplicity in operation, mild condition, low detection limit, high sensitivity, wide linear range and the like.

The invention provides a preparation method for a thermo-sensitive type molecularly imprinted polymer with a metal frame. The method is used for enriching or sensing bovine hemoglobin and comprises the following steps: preparing a metal frame compound with upconversion fluorescence; introducing a thermo-sensitive monomer. According to the preparation method for the thermo-sensitive type molecularly imprinted polymer of the metal frame, UCNPs with strong fluorescence and MOF with larger specific surface area are introduced into a molecular imprinting technology, and a prepared composite material can be used for enriching or sensing the bovine hemoglobin. Meanwhile, the thermo-sensitive monomer is introduced, so that the composite material has a temperature-based reversible adsorption/release function and has a broad application prospect.

The invention discloses a construction method of an impedance type electrochemical sensor based on a molecularly imprinted polymer with a magnetic surface and an application of the sensor in protein detection, and belongs to the technical field of electrochemical sensing. The construction method comprises the following steps: synthesizing the molecularly imprinted polymer on the surface of a magnetic nano particle by taking hemoglobin as a template molecule and dopamine as a monomer and by using the self polymerization performance and the good adhesive capacity of the dopamine; eluting the hemoglobin, so as to prepare the molecularly imprinted polymer with the magnetic surface, wherein the molecularly imprinted polymer with the magnetic surface has the good identification effect on the hemoglobin; and fixing the polymer on the surface of a magnetic glassy carbon electrode under the action of a magnetic field, so as to construct a hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface, wherein the hemoglobin sensing interface based on the molecularly imprinted polymer with the magnetic surface is used for hemoglobin detection. The constructed electrochemical sensor based on the molecularly imprinted polymer with the magnetic surface is simple in method, high in hemoglobin detection sensitivity and good in selectivity.

The present invention provides an electrochemical detection method for content of glycosylated hemoglobin in the blood, and is characterized in that: whole blood specimen is diluted by combined liquid ranging from 30 times to 70 times and passes through a glycosylated protein recognizer, electric activated small molecules are filled into the effluent liquid from the glycosylated protein recognizer, and electrochemical response of the liquid is detected by chemically modified electrodes after the filling, thereby obtaining the concentration of the blood glucose. The glycosylated protein recognizer is eluted by elute solvent, and the electrochemical response of the elute solvent is detected and the concentration of the glycosylated hemoglobin is obtained, thereby obtaining the content of the glycosylated hemoglobin of the sample after calculation. As the electrochemical detection is simple in operation, lower in cost and rapid in the analysis, the present invention is expected to realize the rapid determination of the clinical glycosylated hemoglobin and the rapid determination of the glycosylated hemoglobin after separation and elution.

The invention relates to an enzymatic test kit for glycosylated hemoglobin, which is composed of hemolysis buffer solution, reagent R1a, reagent R1b and reagent 2; wherein, the hemolysis buffer solution is 10 to 1000mmol/ L N- cycloethyl-2-aminoethanesulfonic acid, 10 to 1000mmol/L MOPS and 1 to 100g/L polyethylene glycol oxide dodecyl ether; the reagent R1a is 100 to 10000 kU/L metalloproteinase, 0.1 to 10mmol/L 2-(iodophenyl)-3-(2, 4- dinitrobenzene)-5-(2, 4-disulfophenyl)-2H tetrazole sodium salt; the reagent R1b is 0.1 to 10mmol/L MES and 0.5 to 50mmol/L CaCl2; the reagent 2 is 1 to 100kU/L fructose valine oxidase with high activity, 30 to 600kU/L POD, 0.01 to 0.5mmol/L DA-64 and 10 to 1000mmol/L Tris-HCl. The test kit coincides with the characteristics of rapidness, large batch size and economy which are required by the glycosylated hemoglobin clinical tests.

The invention is applicable to the technical field of information processing, and provides a detecting method for a muscle fatigue level and equipment. The method includes the following steps: obtaining a biometric signal of a user; obtaining a wavelet amplitude mean value of a hemoglobin concentration signal through a preset wavelet averaging algorithm; performing Fourier transform on a surface myoelectric signal to obtain a surface myoelectric frequency domain curve of the surface myoelectric signal, and determining a median frequency value according to the surface myoelectric frequency domain curve; leading the wavelet amplitude mean value, median frequency value and a blood oxygen saturation concentration signal mean value into a preset fatigue level calculation model, and determininga current fatigue characteristic value of the user; and based on a preset fatigue level index table, determining the fatigue level corresponding to the fatigue characteristic value. According to the method, the active state of muscle fibers of current user muscles can be determined through the myoelectric signal, and the metabolic state of the muscles can be determined through the blood oxygen signal, so that a more comprehensive judgment on the muscle fatigue state is realized, and the detection accuracy of the muscle fatigue level is improved.

A method for demonstrating pre-emergent pimples is disclosed. The method includes providing a camera having a 25 mm macro lens and a linear polarizing filter including from 10 to 50 narrow band filters in the spectral range of 400-970 nm, and a light source having a linear polarizer oriented vertical to a polarization plane of the camera polarizer; taking a sequence of images of the face of a subject with the camera; creating an erythema map of the imaged area using oxy-hemoglobin values at each corresponding pixel; taking a visible-light image of the face of the subject; and comparing the images to see pre-emergent pimples. The method enables consumers to see pre-emergent pimples and treat them, which results in preventing the pimples from emerging or reducing the emergence of the pimples.

The invention relates to a surface enhanced Raman scattering technology-based composite material and a preparation method thereof. The composite material comprises microspheres modified with first target molecules on the surfaces and a second component modified with Raman signal molecules and second target molecule-modified polymers on the surface. In a solution containing a substance to be detected, the substance to be detected, the microspheres and the second component are bonded to the second target molecules through the first target molecules so that the composite material which can be easily precipitated through centrifugation is obtained. The invention also discloses the preparation method and a use of the composite material. The composite material can be used for detecting a human prostate-specific antigen (PSA), a human ferrohemoglobin (Hb), a procalcitonin (PCT) or disease markers (such as tumor markers, cardiovascular disease markers, senile dementia markers, mycoplasma and chlamydia), can effectively reduce false positive and false negative effects and has advantages of high sensitivity, simpleness, fastness and low cost.

The present invention provides a measurement method utilizing immunochromatography, an immunochromatographic test strip, and a reagent kit of immunochromatography capable of accurate short-time measurement of an analyte in blood with simple operations as compared to the conventional methods. The present invention provides a method of measurement by immunochromatography in which concentrations of an analyte and hemoglobin in the same sample are measured by immunochromatography to perform hematocrit correction of a measurement value of the analyte by using a measurement value of hemoglobin, as well as a test strip and a reagent kit for immunochromatography.

Disclosed is a hemoglobin measurement method which can measure hemoglobin, particularly stabilized hemoglobin A1c which can be used as an indicator for the diagnosis of diabetes, within a short periodof time and with high accuracy. Also disclosed is an electrophoresis apparatus which can be used suitably in the measurement method. Specifically disclosed is a method for the measurement of hemoglobin by electrophoresis, wherein the method employs a migration path having an inner surface immobilization-coated with a cationic substance or a migration path having an inner surface made of a cationic material and also employs a buffer solution containing a water-soluble polymer having an anionic group as a running buffer.

The invention discloses a recombinant plasmid for expressing vitreoscilla hemoglobin in a cell and a construction method of the recombinant plasmid, a genetic engineering bacterium of Gluconobacter oxydans (G.oxydans) and a construction method and application of the genetic engineering bacterium. The recombinant plasmid comprises a vitreoscilla hemoglobin gene vgb, a PtufB promoter of the Gluconobacter oxydans (G.oxydans) and a suitable carrier, wherein the genetic engineering bacterium is obtained by transforming recombinant plasmid into a host bacterium. The genetic engineering bacterium can be used for expressing the vitreoscilla hemoglobin in the cell, can be used for improving the yield of biomass and catalysate 1, 3-dihydroxyacetone under the condition of not changing the existing equipment and energy consumption pressure and provides an effective path for solving the problem of in sufficient oxygen supply in the cell cultivation and catalytic process of the Gluconobacter oxydans (G.oxydans).

The invention provides a polypeptide vesicle carried with hemoglobin and a preparation method thereof. The hemoglobin is wrapped in the vesicle, and the wall of the vesicle consists of polylysine-polyphenylalanine di-block copolypeptide. Firstly, hexyl amine is taken as an initiating agent, poly(Z-lysine) is obtained through ring-opening polymerization of N-benzyloxycarbonyl(Z)lysineN-carbonyl carboxylic acid anhydride, then a terminal amino group of the poly(Z-lysine) is used to initiate the ring-opening polymerization of phenylalanineN-carbonyl carboxylic acid anhydride to obtain poly(Z-lysine)-polyphenylalanine, the poly(Z-lysine)-polyphenylalanine is deprotected and then is self-assembled in an aqueous solution to form a vesicle and wrap the hemoglobin, and the vesicle with the hemoglobin is obtained. The vesicle is formed by directly assembling amphiphilic block copolypeptide in the aqueous solution, and no organic solvent needs to be used, so the biological activity of the hemoglobin is better maintained.