62 results about "Spider Proteins" patented technology

The invention provides a bio-based adhesive and a preparation method thereof. Inspired by a spider thread structure, biomass adhesive interface enhancement based on a tannic acid adhesion group is combined with construction of a differential phase separation structure by biomass-induced polylipoic acid depolymerization, and a brand new strategy of adhesion-cohesion synergistic enhancement of the adhesive strength of the adhesive is realized. According to the invention, tannic acid and carboxymethyl chitosan are introduced into a lipoic acid-based adhesive, so that the purpose of synergistically enhancing the bonding performance of the adhesive through adhesion-cohesion is achieved, and the defects of weak cohesion, low bonding strength, poor water resistance, poor moisture resistance and the like of the conventional biomass adhesive are overcome. Benefited from a new strategy of adhesion-cohesion synergistic enhancement, the biomass adhesive has good adhesive strength in various environments and materials. In addition, the biomass adhesive also has the characteristics of biological safety, antibacterial and mildew-proof properties and recoverability, so that the product has wider application and potential compared with the previous product, and has a good application prospect.

The invention belongs to the technical field of biology, and particularly discloses a high-density fermentation method and application of recombinant spider silk protein, and the high-density fermentation method comprises the following steps: firstly, transforming an expression vector carrying spider silk protein genes into modified pichia pastoris cells to obtain a strain; the activated strain is subjected to expanding culture, high-density fermentation culture and purification, and recombinant spider silk protein is obtained; wherein the transformation means of the pichia pastoris cell is to integrate Vitreoscilla hemoglobin Vhb gene into the pichia pastoris cell by utilizing a gene recombination technology, and the expression of the Vitreoscilla hemoglobin Vhb gene is improved by carrying out metabolic engineering transformation on the pichia pastoris cell for expression and optimizing the regulation and control of staged fermentation parameters, so that the expression efficiency of the Vitreoscilla hemoglobin Vhb gene is improved. The problems that in existing recombinant protein fermentation, oxygen dependence is high, product degradation is serious, purity is low, and large-scale stability is poor are solved, the yield and purity of recombinant spider silk protein are guaranteed, and large-scale production of the recombinant spider silk protein is facilitated.

Cosmeceutical compositions comprising a major ampullate spidroin protein (MaSp)-based fiber are disclosed. Articles comprising the compositions are further disclosed.

The application discloses a kind of imitative spider silk cellulose gel fibers and its preparation method and application, method includes: using DMAc to activate cellulose powder and obtain activated cellulose powder;Activated cellulose powder is added in DMAc / LiCl solvent and dissolved, and obtain cellulose solution;Using microfluidic equipment, cellulose solution is injected into deionized water and solidified, and washing is obtained cellulose gel fiber;Cellulose gel fiber is soaked in PVA-PAA precursor liquid, and then placed under ultraviolet lamp irradiation, then freeze and thaw, and obtain cellulose / PVA / PAA hydrogel fiber;Cellulose / PVA / PAA hydrogel fiber is pre-stretched, then soaked in CuSO4 solution, and then washed with deionized water after soaking, and obtain imitative spider silk cellulose gel fiber.The imitative spider silk cellulose gel fiber provided by the application has strong toughness, flexible conductivity and environmental resistance.

The invention belongs to the technical field of protein, and aims to solve the problem that the application range of spider silk particles as a drug carrier is limited due to the fact that the spider silk particles are in negative net charge under a neutral condition and can only load drugs with positive charges or neutral charges at present. The novel recombinant spider silk protein is prepared on the basis of an N connection sequence of araneus ventricosus MaSp1, has positive net charge under a neutral pH value, can load a negatively charged drug, and expands the application range of the spider silk protein as a drug carrier; and a drug release test result shows that the recombinant spider silk protein is superior to MaSp2-based silk protein particles in the aspect of etoposide delivery.

The invention relates to the technical field of biology, in particular to a recombinant coral-spider silk fusion protein and functional alginate fiber and application thereof. The recombinant coral-spider silk fusion protein provided by the invention comprises the recombinant coral-spider silk fusion protein formed by fusing spider silk protein or rattan silk protein and coral protein. The invention designs a fusion protein only containing spider silk protein and coral protein. The recombinant coral-spider silk fusion protein is applied to alginate fibers, colored spinning of the alginate fibers can be achieved, and the spun fibers are high in color fastness, superior to original fibers in strength and elongation and bright and stable in color.

The invention belongs to the technical field of biology, particularly discloses a method for preparing spider silk protein-containing composite silk by using transgenic silkworms and application of the spider silk protein-containing composite silk, and particularly provides a method for synthesizing and secreting spider silk protein by using a silkworm silk gland bioreactor. The spider silk protein gene is introduced into a bombyx mori genome in a virus infection manner by utilizing a transgenic bombyx mori technology and is specifically expressed in bombyx mori silk gland cells, and the spider silk protein target gene can be introduced in batches by virtue of a lentivirus transfection method, so that bombyx mori capable of synthesizing and secreting complete spider silk protein can be developed on a large scale; the spider silk protein is synthesized and secreted through bombyx mori silk gland cells and enters silkworm cocoons along with the spinning and cocooning behaviors of bombyx mori, the silk is collected, the spider silk protein-containing composite silk with the bombyx mori silk as the background is obtained, a foundation is laid for mass production of black spider silk protein, and meanwhile a foundation is laid for improving the mechanical performance of the silk.

The invention provides a cement-based material with a spider silk-like microstructure and a preparation method of the cement-based material, and belongs to the technical field of cement-based materials and preparation of the cement-based materials. The cement-based material comprises the following raw materials: double-component cement, reinforced fibers, mineral whiskers and an additive, the two-component cement comprises silicate cement and sulphoaluminate cement. The expansion effect generated by the hydration reaction of anhydrous calcium sulphoaluminate in the double-component cement not only counteracts the partial shrinkage effect generated by the hydration of Portland cement clinker minerals, but also extrudes the reinforced fibers, so that the binding force between the fibers and a cement matrix is increased. Besides, a secondary frame formed by the generated ettringite raphioid crystals and the added mineral whiskers is combined with a main body frame of the reinforced fibers to form a spider-screen-frame-imitating toughening structure, so that the strength of the material can be improved, and the toughness of the material is also improved.

The invention relates to the technical field of biology, in particular to recombinant spider silk protein, modified polyurethane and application of the recombinant spider silk protein and the modified polyurethane. The recombinant spider silk protein provided by the invention comprises spider silk protein or spider silk-like protein; the spider silk protein or spider silk-like protein comprises at least one spider silk globulin NT, at least one spider silk protein repeat region and at least one spider silk globulin CT from the N end to the C end. Research finds that when the recombinant spider silk protein is applied to waterborne polyurethane, the mechanical strength of a polyurethane material is remarkably improved, the mechanical property of the polyurethane material is enhanced, and the polyurethane material is environmentally friendly and non-toxic, has the characteristics of no toxicity, high strength, high elasticity and the like, and keeps the original characteristics of low organic matter emission, no pollution, high elasticity, air permeability and the like at the same time. The recombinant spider silk protein can have a CBM structure, so that the mechanical property can be improved. Fluorescent protein and / or chromoprotein can be fused on the recombinant spider silk protein, polyurethane with different appearances can be obtained, and color combination is firm.

A recombinant spider silk protein, consisting of no more than 800 amino acids, comprising a set of domains arranged according to the formula (NT)-REP-CT, wherein: the optional NT-domain, if present, comprises a sequence of 100 to 160 amino-acid residues derived from the N-terminal domain of a spider silk protein; the REP-domain comprises a sequence of 30 to 600 amino acid residues derived from the repetitive segment of a spider silk protein; and the CT-domain comprises a sequence of 70 to 120 amino acid residues derived from the C-terminal domain of a spider silk protein selected from: a sequence of 72 to 110 amino acid residues derived from the C-terminal domain of a spider silk protein, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D; a sequence having at least 85% identity to SEQ ID NO: 15 or any one of SEQ ID NOs: 62-65 or 67-73; and a sequence having at least 70% identity to SEQ ID NOs: 64 or any one of SEQ ID NOs: 62-65 or 67-73, wherein the sequence comprises at least 7 residues independently selected from K, R, E and D.

The invention provides a spider silk protein modified body easy to purify as well as a modification method and a purification method thereof, a plurality of amino acid residues in flexible regions on two sides of a spider silk protein microcrystalline region are replaced with acidic amino acids or alkaline amino acids, so that the proportion of charged amino acids in the flexible regions is greater than or equal to 7%, and the spider silk protein modified body is obtained; the spider silk protein modified body is purified in the form of an inclusion body through a temperature change process of heating and cooling. The modified body can be simply purified with high recovery rate through simple heating and cooling processes, the total recovery rate is up to 98% or above, the purity is 90%-99% or above, and meanwhile, the formed artificial spider thread is endowed with higher tensile strength by strengthening the interaction of beta-sheet on the two sides of a microcrystal region, especially between different chains. A foundation is further laid for large-scale efficient production and low-cost separation and purification of the high-performance spider silk protein, and the application possibility of the spider silk protein in various fields is greatly enhanced.

A plurality of methods and apparatus for managing deficiencies of the meibomian gland lipid production and delivery, including wearable fabrics configured with a matrix of micro- and nano-electromechanical materials, or composite fibres of yarn that are substantially made from carbon nanotubes, graphene, spider silk, natural silk, denim, cotton, metals, or combination thereof. Other methods and apparatus include infrared and ultrasound energy sources within a kit aimed at personalised therapies for improving overall health of the eyelids, while providing for adjunctive relief for associated dry eye symptoms. The management kit may comprise a wearable fabric or spectacle frame configured with infrared emitters and sensors, a handheld imaging device and software containing dosage and administration information coupled with information on improving eyelid hygiene which is reviewed and periodically updated using user-specific information.

The present invention relates to a novel injectable protein delivery system and a method for delivering one or more therapeutic agents to the central nervous system (CNS) to promote axonal regeneration. The system is based on the use of a recombinant spider silk protein, referred to as spiroin-SpyTag, which undergoes a rapid transition from a sol state to a gel state when exposed to ultrasonic treatment and incubated at body temperature. This unique characteristic allows the material to be easily injected into a specific target tissue. The methods disclosed herein can deliver a protein therapeutic agent covalently conjugated to spiroin-SpyTag to a subject affected by a CNS disorder or injury. In addition, methods of making such injectable protein delivery systems are fast, convenient, and economical and effective.

The application provides an aggregated spider silk protein nanoparticle, a drug delivery system and a preparation method and application thereof, and belongs to the technical field of biological medicines.The application provides application of the aggregated spider silk protein in preparation of a drug delivery system.The application researches and finds that the aggregated spider silk protein nanoparticle prepared by using the amino acid sequence such as SEQ ID NO.1, SEQ ID NO.3 or SEQ ID NO.5 has good colloidal stability and good safety, and is a novel drug delivery system.The drug delivery system loaded with a tumor drug has high encapsulation efficiency and drug loading capacity for the tumor drug, the encapsulation efficiency is more than 60%, has a slow-release effect on the tumor drug, and is an excellent drug delivery system.The research finds that compared with the simple tumor drug, the drug delivery system loaded with the tumor drug has a significantly improved killing effect on tumor cells, and can effectively treat tumors.

The invention discloses a natural spider silk-based self-adaptive surgical suture and a preparation method thereof, and belongs to the technical field of biomedical materials. According to the surgical suture, natural spider silk serves as a raw material, protein chains in the spider silk are rearranged in order by regulating and controlling the moisture state, applying axial pre-stretching strain and conducting drying, annealing and shaping under the limited condition, and the suture material with the high strength and the environmental response characteristic is obtained. The surgical suture is based on natural spider silk, has excellent biocompatibility and inherent biodegradability, becomes an ideal candidate material for biomedical application, and is successfully applied to suture of skin, stomach and other parts. According to the invention, the spider silk protein chains are rearranged into the superfine nanofibers, so that the engineered natural spider silk suture with remarkably enhanced mechanical and functional properties can be produced, and the monofilament can realize the breaking strength of 2.0 GPa, the toughness of 480 MJ m < 3 > and the driving stress of 92.6 MPa.

This invention discloses a self-contained conical microchannel and its design method. The microchannel includes a conical channel with a biomimetic fishtail structure and a biomimetic spider silk structure on its surface. The biomimetic fishtail structure is a fishtail-shaped groove formed by bending the base of a triangle with a consistent orientation inward. The biomimetic spider silk structure is a spider silk-shaped groove composed of hemispherical and conical channels. Multiple biomimetic fishtail structures are arranged in an array, and multiple biomimetic spider silk structures are arranged in an array, alternating between the fishtail and spider silk arrays. This invention combines the biomimetic fishtail and spider silk structures on the surface of the conical channel. The fishtail structure enables self-collection of liquid within the conical microchannel, while the spider silk structure enables rapid self-transportation of liquid. This technology can be applied to micro-drug delivery without external force, microfluidic devices, and friction interfaces.

The invention discloses a spider silk collecting device and process, and particularly relates to the technical field of spider silk, the spider silk collecting device comprises a telescopic rod composed of an expansion sleeve and two long rods, the head of the telescopic rod is provided with an installation assembly, the front portion of the installation assembly is provided with flannelette fixed through an opening clamp, and the surface of the flannelette is provided with three-dimensional fluff. And collecting the whole cobweb by means of the three-dimensional fluff. According to the spider silk collecting device and technology, in the storage technology, the flannelette attached with spider webs is fixed to the foaming plates through insect needles, spider silk stress and adhesion caused by flannelette bending are effectively avoided, and through the small foaming plates fixed to the four corners of the foaming plates, when a plurality of foaming plates carrying spider silk are placed in a carton, the spider silk collecting effect is good, and the spider silk collecting effect is good. Stable intervals are formed, and extrusion damage during transportation or storage is prevented.

The application provides a kidney-tonifying and anti-fatigue polypeptide composition and a preparation method thereof, belongs to the technical field of medicinal biological peptide complexes, and the composition comprises oyster polypeptide, holothurian polypeptide, spider silk and blue ears grass-pentamerous fruit composite extract, cistanche-dendrobium officinale composite extract, ginseng extract and deer penis extract; the oyster polypeptide is a common enzymolysis product of oyster meat by trypsin and bovine chymotrypsin; the holothurian polypeptide is a common enzymolysis product of holothurian body wall by alkaline protease, collagenase type II and subtilisin; the spider silk and blue ears grass-pentamerous fruit composite extract is a product obtained by the following steps: subjecting spider silk and blue ears grass and pentamerous fruit to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a SA sulfonic acid silica gel strong cation exchange column; the cistanche-dendrobium officinale composite extract is a product obtained by the following steps: subjecting cistanche and dendrobium officinale to enzymolysis, extracting with an ethanol aqueous solution, and purifying with a phenylboronic acid affinity column-polyamide column series connection column; and the composition realizes the synergistic effect of kidney tonification and anti-fatigue.

The application discloses a kind of recombinant spider silk protein fibers based on SpiCE-DS8 protein and preparation method thereof, belong to polymer fiber material technical field.The application discloses the key functional domain SpiCE-DS8-S (KGGLDILGGLLKLSKAGKDGKNFF) of SpiCE-DS8 protein, which can promote the self-assembly and fiber formation of silk protein.By SpiCE-DS8 protein as the raw material of spinning solution, the recombinant spider silk protein fiber prepared has significant improvement in strength, toughness and elongation, which provides the possibility for improving the mechanical properties of in vitro recombinant silk, and the method of the application simplifies the spinning process, continuous spinning can be realized, which is beneficial to industrial production.

The subject invention pertains to a novel injectable protein delivery system and methods for delivering one or more therapeutic agents in the central nervous system (CNS) for promoting axon regeneration. This system is based on the use of a recombinant spider silk protein called spidroin-SpyTag, which undergoes a rapid transition from a sol state to a gel state when exposed to ultrasound treatment and incubated at body temperature. This unique characteristic allows the easy injection of the material into a specific target tissue. The methods herein disclosed allow the delivery of protein therapeutics covalently conjugated to the spidroin-SpyTag to a subject affected by a CNS disorder of injury. Additionally, the method for fabricating this injectable protein delivery system is rapid, convenient, and cost-efficient.

A recombinant spider silk protein comprises an N-terminal (NT) domain, a repetitive region (REP) domain and a C-terminal (CT) domain. The REP domain comprises at least 100 amino acids derived from the repetitive region of a spider silk protein of Larinioides sclopetarius. The NT domain is derived from the NT domain of a spider silk protein other than the spider silk protein, from which the REP domain is derived and / or the CT domain is derived from the CT domain of a spider silk protein other than the spider silk protein from which the REP domain is derived.

The invention relates to the technical field of bio-based functional fiber manufacturing, in particular to a preparation method of a modified spider silk protein enhanced lyocell fiber, which comprises the following steps: firstly, carrying out spider silk protein expression through a pichia pastoris strain GS115 / pPIC9K-spider silk gene; secondly, performing biomimetic mineralization treatment on spider silk protein, and performing ultrasonic-assisted mineralization to form a silicon dioxide coating layer; then preparing a spinning solution to realize uniform mixing of the mineralized spider silk protein and cellulose; coaxial spinning is carried out through micro-fluidic spinning equipment, and skin-core structure fibers are constructed; and finally inducing the formation of a beta-folding structure in an ethanol / water / calcium chloride coagulating bath. The process breaks through the technical bottlenecks of phase separation, heat sensitivity inactivation and uneven dispersion of spider silk protein and cellulose, reduces the production cost, and provides a high-performance fiber material for the high-end textile and biomedical fields.

The invention relates to the technical field of recombinant protein design and synthesis, and particularly discloses a recombinant spider silk protein and a synthesis method thereof. The recombinant spider silk protein provided by the invention is subjected to N-terminal and C-terminal structural domain replacement of different spider species and different spider silk types, intermediate functional domain repetitive sequence large fragment recombination and other optimization designs, and a series of recombinant spider silk proteins are obtained through a cell-free protein synthesis method and prokaryotic fermentation expression; compared with natural spider dragline silk protein or other existing recombinant spider silk protein, the spider silk protein has good composite mechanical performance and high temperature resistance, meanwhile, the molecular weight is small, the total expression quantity and soluble expression are remarkably improved, and a novel raw material and a design synthesis method of the recombinant spider silk protein are provided for development of high-performance biological materials.

Cosmeceutical compositions comprising a major ampullate spidroin protein (MaSp)-based fiber are disclosed. Articles comprising the compositions are further disclosed.

The invention discloses a method for producing recombinant spider silk protein based on microalgae photosynthetic expression and non-chromatographic purification, the recombinant spider silk protein and application, and belongs to the technical field of recombinant spider silk protein production. The method comprises the following steps: designing a target recombinant spider silk protein structure, taking a spider dragline silk protein repetitive unit as a core, matching with an end-side elastin structural domain, inserting an amber termination codon TAG at a specified glycine site of the repetitive unit, and introducing non-natural amino acid containing a click chemical functional group by virtue of a gene codon extension technology; the method comprises the following steps: artificially synthesizing an optimized gene expression cassette, constructing a chlamydomonas reinhardtii expression system, synchronously constructing an unnatural amino acid insertion system, co-transforming the gene expression cassette and the unnatural amino acid insertion system into chlamydomonas reinhardtii, screening positive clones, carrying out photosynthetic culture induced expression, and carrying out pH-induced reversible phase separation in combination with magnetic nanoparticle click chemical non-chromatographic purification. Finally, the high-purity recombinant spider silk protein is prepared, and a technical basis is provided for industrialization of biological materials.

The invention discloses a spider silk-like hybrid polymer fiber and a preparation method thereof, and belongs to the field of synthetic fibers, and the preparation method comprises the following steps: dissolving, heating and stirring a carboxyl-containing polymer until the carboxyl-containing polymer is completely swelled, and adding an acid solution for adjustment to obtain polymer gel; adding an amino-containing polymer into the polymer gel, heating and uniformly stirring to obtain hybrid gel, and centrifuging the hybrid gel to obtain a hybrid gel spinning precursor stock solution; extruding the hybrid gel spinning precursor stock solution into an anti-solvent coagulating bath, collecting fibers, and obtaining hybrid polymer fibers after moisture absorption is balanced; and carrying out pre-stretching and moisture absorption balance to obtain the spider silk-like hybrid polymer fibers with different uniaxial orientation and crystallinity structures. According to the invention, continuous preparation of the fiber is realized by combining the high-molecular polymer with anti-solvent induced phase separation and pre-stretching strain programming, the method is simple, the raw material source is environment-friendly, and the prepared fiber has comprehensive characteristics similar to spider silk.

The invention provides a preparation method of spider protein synthetic leather, which comprises the following steps: S10, preparing surface layer slurry: selecting spider protein powder with the average particle size D50 of 1.0-10.0 microns, dispersing the spider protein powder in a polar organic solvent for pre-infiltration treatment to obtain suspension liquid, and drying the suspension liquid to obtain the surface layer slurry; then mixing the suspension liquid with a polyurethane surface layer resin solution, and uniformly dispersing to prepare surface layer slurry containing spider protein powder; s20, preparing inner-layer slurry: preparing polyurethane inner-layer slurry which does not contain arachnoid protein powder; s30, dry molding: coating release paper with the surface layer slurry, drying the surface layer slurry to form a surface layer, and controlling the ratio of the dry film thickness of the surface layer to the average particle size D50 of the spider protein powder to be (5: 1)-(10: 1); and then coating the surface layer with the inner layer slurry, drying to form an inner layer, attaching base cloth to the inner layer, and curing and stripping to obtain the spider protein synthetic leather. The synthetic leather prepared by the preparation method has good mechanical properties, good breathable effect and excellent low-temperature folding resistance.

To provide an improved method for purification of recombinant spider silk protein, thereby enhancing solubility and increasing silk protein recovery yield.SOLUTION: Provided is a method for improving solubilization, extraction, and isolation of a recombinant spider silk protein using a salt and alcohol buffer. Provided is a method for solubilizing a recombinant spider silk protein from a host cell, comprising: providing a cell culture comprising a host cell, the host cell expressing a recombinant spider silk protein; recovering an insoluble portion derived from the cell culture, the insoluble portion comprising the recombinant spider silk protein; and adding the insoluble portion of the host cell to a solution comprising a salt and an alcohol, thereby solubilizing the recombinant spider silk protein in the solution.SELECTED DRAWING: Figure 1