114results about How to "Easy screening" patented technology

The present invention provides methods of re-engineering or re-shaping an antibody from a first species, wherein the re-engineered or re-shaped antibody does not elicit undesired immune response in a second species, and the re-engineered or re-shaped antibody retains substantially the same antigen binding-ability of the antibody from the first species. In accordance with the present invention, a combinatorial library comprising the CDRs of the antibody from the first species fused in frame with framework regions derived from a second species can be constructed and screened for the desired modified antibody. In particular, the present invention provides methods utilizing low homology acceptor antibody frameworks for efficiently humanizing an antibody or a fragment thereof. The present invention also provides antibodies produced by the methods of the invention.

Methods for identifying discrete monomer domains and immuno-domains with a desired property are provided. Methods for generating multimers from two or more selected discrete monomer domains are also provided, along with methods for identifying multimers possessing a desired property. Presentation systems are also provided which present the discrete monomer and / or immuno-domains, selected monomer and / or immuno-domains, multimers and / or selected multimers to allow their selection. Compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.

The present invention provides thrombospondin, thyroglobulin and trfoil / PD monomer domains and multimers comprising the monomer domains are provided. Methods, compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.

A new method of cell fixation using a mild denaturing heat and the fixative PermiFlow™ or similar fixative that preserves cell morphology, light scatter profiles, nucleic acid content, cell surface epitopes and unmasks internal epitopes previously not available. The method can be combined with analysis of each of these parameters by flow cytometry and thus has application in drug screening and patient or tumor monitoring protocols.

A biosensor which comprises a substrate (101) having a buried electronic sensing element and a substrate surface (124) above the buried electronic sensing element; a structured top layer (125) covering the substrate surface (124), having a top surface above the substrate surface (124), and comprising at least one stimulation and / or sensing electrode (116) and a channel (121) for holding the biomolecule by means of suction through said channel (121) arranged between the top surface (125) and the substrate surface (124), the sensing electrode (116) being electrically coupled to the electronic sensing element; wherein the top surface (125) is provided for placing a biomolecule present in a sample solution thereupon, the sensing electrode (116) is provided for sensing electrical variations in and presence of the biomolecule.

A media or recorded event editing, screening, and communications trailer provides portable means by which editing, screening, and communications equipment can be brought on-site or otherwise to provide greater efficiency and less delay in processing film or other media. A large trailer such as that used in tractor / trailer rigs provides the basis for a combined facility incorporating a projection room, a screening area, several editing consoles, a refreshment area, an equipment area, a foyer, and an editing area. Slideouts provide increased interior volume space for greater working comfort yet are sturdy and stable both in their deployed and collapsed conditions. A communications facility incorporated into the trailer provides local and / or global communications networking and access as well as audio, video, and / or audio / video signal communication capacity.

The invention provides an improved method for detecting cancer or a precancerous condition in a sample using an oxidation agent, such as galactose oxidase, and an aldehyde detection agent, such as Schiff's reagent that does not require the sample to be immobilized onto a solid support. The invention also provides kits comprising the components necessary for carrying out the methods of the invention.

The present disclosure relates to semiconductor based Josephson junctions and their applications within the field of quantum computing, in particular a tuneable Josephson junction device has been used to construct a gateable transmon qubit. One embodiment relates to a Josephson junction comprising an elongated hybrid nanostructure comprising superconductor and semiconductor materials and a weak link, wherein the weak link is formed by a semiconductor segment of the elongated hybrid nanostructure wherein the superconductor material has been removed to provide a semiconductor weak link.

The present invention relates to bacterial luciferase expression cassettes suitable for conferring bioluminescence properties on Gram-positive bacteria, cells transformed with such cassettes, and methods of making and using such cassettes.

A combinatorial library of fluorescent compounds useful as organelle-specific probes are produced by reacting an aldehyde with a 2- or 4-methylpyridinium salt.

Improvements in calorimetric assays, surfaces for arraying microcolonies and instrument hardware for screening mutagenized enzymes and proteins in a solid phase format are presented. These improvements permit new enzyme activities to be screened. New filter membrane materials and formats for arraying the microcolonies provide higher throughput, better solvent resistance and ease of handling. Modifications to the instrument heating and illumination systems provide improved temperature control and a more compact, folded light path.

An electric circuit is applied to an object having a curved surface. The curved surface of the object is divided into sections, and the circuit is applied one section at a time. The circuit is formed between layers of dielectric material. The dielectric is applied by a computer-controlled device, which controls the position of a spray head and the rotation of the object, such that the spray head is held substantially perpendicular to the surface of the object at all times, and such that a controlled thickness of dielectric material can be deposited. The fine-featured circuits formed by the invention are rugged, and can be used on objects intended to be exposed to harsh environments.

The present invention provides methods and vector systems for the generation of chimeric recombinant adenoviruses. These hybrid adenoviruses contain a genome that is derived from different adenovirus serotypes. In particular, novel hybrid adenoviruses are disclosed with improved properties for gene therapy purposes. These properties include: a decreased sensitivity towards neutralizing antibodies, a modified host range, a change in the titer to which adenovirus can be grown, the ability to escape trapping in the liver upon in vivo systemic delivery, and absence or decreased infection of antigen presenting cells (APC) of the immune system, such as macrophages or dendritic cells. These chimeric adenoviruses thus represent improved tools for gene therapy and vaccination since they overcome the limitations observed with the currently used serotype subgroup C adenoviruses.

PCT No. PCT / GB96 / 00804 Sec. 371 Date Apr. 6, 1998 Sec. 102(e) Date Apr. 6, 1998 PCT Filed Apr. 2, 1996 PCT Pub. No. WO97 / 04424 PCT Pub. Date Feb. 6, 1997A coin validator is provided with at least two reference positions (U, D) for determining a diameter related characteristic of a coin being validated. In order to reduce the running to the testing station, the timing of a trailing point of the coin passing a first reference position (U) is used to determine the diameter related characteristic. Embodiments using optical inductive and piezo-electric sensors associated with the reference positions are disclosed. An inductive sensor for a coin validator comprises an elongate coil, which, when in use, is arranged such that the magnetic field is substantially constant across the width of the passageway. The use of coils of this type have the advantage of wrap around coils but enable the coin passageway to be shallower and be opened. A coin validator is described wherein the backwall of a coin passageway is movable to and fro so that the depth of the coin passageway can be adjusted. In an embodiment, a cam bears against the backwall of the coin passageway to set the depth thereof.

Nucleic acid sequences and corresponding translated products of novel mutant forms of the Drosophila hid gene are described. Such sequences and products are useful in screening methods for identifying and testing antagonists of Hid phosphorylation, in particular, compositions modifying MAPK phosphorylation of Hid.

The present invention provides a screening method for somatic cell nuclear reprogramming substances, which comprises (a) a step for bringing into contact with each other a somatic cell comprising a gene wherein a marker gene is present at a position permitting expression control by the expression control region of an ECAT gene, and a test substance, and (b) a step following the aforementioned step (a), for determining the presence or absence of the emergence of cells expressing the marker gene, and selecting a test substance allowing the emergence of the cells as a somatic cell nuclear reprogramming substance candidate, and the like.

In vitro methods are disclosed that rely on a novel phenomenon of secondary sprouting by cultured endothelial precursor cells, after angiogenic-like tube formation and collapse have occurred on a basement membrane matrix. Particularly disclosed is an in vitro method of isolating and expanding, from a mixed population of mammalian cells originating from a tissue sample, a cellular population enriched for endothelial sprout cells, including cells having one or more physiological and / or immunological features of endothelial precursor cells. Also disclosed is an in vitro method for a screening a substance for potential proangiogenic or antiangiogenic activity.

The present invention provides methods of re-engineering or re-shaping an antibody from a first species, wherein the re-engineered or re-shaped antibody does not elicit undesired immune response in a second species, and the re-engineered or re-shaped antibody retains substantially the same antigen binding-ability of the antibody from the first species. In accordance with the present invention, a combinatorial library comprising the CDRs of the antibody from the first species fused in frame with framework regions derived from a second species can be constructed and screened for the desired modified antibody. In particular, the present invention provides methods utilizing low homology acceptor antibody frameworks for efficiently humanizing an antibody or a fragment thereof. The present invention also provides antibodies produced by the methods of the invention.

The present invention provides a new approach to creating novel polynucleotide sequences by point mutation and recombination in vitro of a set of parental sequences. The new polynucleotide sequences can be useful in themselves or they can be used for the templated synthesis of polymers, e.g. polypeptides composed of α-amino acids as occurring naturally in protein synthesis or polymers comprised of β-amino acids or other building blocks as described (Templated molecules).

The present invention relates to a system and a method for quickly and accurately identifying and classifying resistance genes of a plant from a protein or DNA sequence. In order to identify and classify resistance genes of a plant using a hidden marcov model, conceived is a profile matrix made using a protein sequence of a domain which is encoded by the resistance genes, and a system for identifying the domain of the resistance genes using the profile matrix and classifying the resistance genes by domain combination. The present invention enables effective identification and classification of the resistance genes of a plant using the profile matrix and program, of which the nucleotide base sequence or protein sequence is detected.

Methods for identifying discrete monomer domains and immuno-domains with a desired property are provided. Methods for generating multimers from two or more selected discrete monomer domains are also provided, along with methods for identifying multimers possessing a desired property. Presentation systems are also provided which present the discrete monomer and / or immuno-domains, selected monomer and / or immuno-domains, multimers and / or selected multimers to allow their selection. Compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.

The invention provides methods for quickly and easily screening mixed cell samples for a pharmacodynamic effect to a drug or test agent.

A method and system for the in situ synthesis of a combinatorial library including impregnating a first component with a second component. The method and system advantageously may be employed in the synthesis of materials for screening for usefulness as a catalyst.

A method for identifying stem-loop structures within a genome is provided. A plurality of stem-loop structures, compounds of stem-loop structures, pharmaceutical compositions of stem-loop structures, and treatment methods for affecting a condition or disease in an organism using stem-loop structures is provided. The method is for rapidly identifying and screening small inhibitory stem-loop structures of RNA or DNA sequences of any genome, wherein those sequences or combinations thereof can be administered to obtain a desirable biological affect in a human or other organism for treatment of a condition or a disease. The method is used for rapidly identifying and screening small inhibitory stem-loop structures of a viral RNA (viRNA), wherein the viRNA's prevents death in transfected cells programmed for cell death thus providing siRNA-type compositions for use in treating inflammatory conditions in humans or other species.

This invention generally relates to the nucleic acid sequences of a novel gene FAIM that encodes an apoptosis inhibiting protein. Furthermore, this invention relates to methods of identifying and testing antagonists of FAIM activity and screening for inter- and intra-specific homologs and mutants of FAIM.

The present disclosure relates to semiconductor based Josephson junctions and their applications within the field of quantum computing, in particular a tuneable Josephson junction device has been used to construct a gateable transmon qubit. One embodiment relates to a Josephson junction comprising an elongated hybrid nanostructure comprising superconductor and semiconductor materials and a weak link, wherein the weak link is formed by a semiconductor segment of the elongated hybrid nanostructure wherein the superconductor material has been removed to provide a semiconductor weak link.

The invention relates to a primer, a probe, a composition and a method for screening and identifying MLL rearrangement correlated fusion genes by utilizing a multi-fluorescent polymerase chain reaction (PCR) technology. The screened MLL rearrangement correlated fusion genes comprise thirteen relatively common fusion genes, i.e., MLL-AF9, MLL-AF6, MLL-AF4, MLL-AF1P, MLL-AF1Q, MLL-AF10, MLL-AF17, MLL-AFX1, MLL-ELL, MLL-ENL, MLL-SEPT6, MLL-CBP and dup MLL. The primer, the probe, the testing combination way and the detection method are convenient, economical, fast, good in specificity, high in sensitivity and large in flux, thus being suitable for clinical test of large-batch samples.

The invention discloses a shakeout machine facilitating recycling treatment of old sand and scrap iron and shakeout. The shakeout machine comprises crushing devices and a screening device, wherein thecrushing devices comprise first rotary motors and crushing rollers arranged at one output ends of the rotary motors. The screening device comprises a screening plate which is formed by two electromagnetic suction plates, baffles are arranged on the two sides of the screening plate, second rotating motors are arranged on the baffles, sand guide rollers are arranged at the output ends of the secondrotary motors, and sand collecting boxes are arranged at the bottom ends of the two electromagnetic suction plates of the screening plate. The device can be used for carrying out crushing treatment on the old sand generated in the shakeout process of casting, so that the falling of the old sand is facilitated, and the old sand is prevented from being pasted on the inner wall of a sand collectinghopper or blocking a sand outlet, and meanwhile, the scrap iron in the old sand can be screened and separated, so that the old sand and the scrap iron can be conveniently recycled and used, and the utilization of casting material resources is optimized.