1801results about How to "High affinity" patented technology

The invention discloses an injection filler and a preparation method and application thereof, the filler is polycaprolactone particles, the polycaprolactone particles are spherical particles, the particle size of the spherical particles is 20-100 [mu] m, and the polycaprolactone particles have rough surfaces or unsmooth surfaces. The polycaprolactone microspheres have rough surfaces or unsmooth surfaces, so that the contact area with cells is increased, the adhesion ability and residence time of the cells are improved, the affinity of the polycaprolactone microspheres is improved, and the growth of collagen can be stimulated and accelerated. In the preparation process, the dosage of each component is scientifically screened, and on the basis of controlling the viscosity of a water-phase solution, the spherical polycaprolactone particles which are regular, rough in surface and uniform in particle size are obtained in cooperation with a rapid membrane emulsification method.

The invention belongs to the field of molecular biology and immunology, and particularly relates to an anti-CD3 nano antibody capable of activating the activity of T cells as well as a preparation method and application of the anti-CD3 nano antibody. The invention provides a preparation method of the anti-CD3 nano antibody. The preparation method comprises the following steps: constructing a CD3 plasmid and an overexpression cell line, and immunizing alpaca to obtain peripheral blood mononuclear cells (PBMC) of the alpaca; extracting RNA (Ribonucleic Acid) and carrying out reverse transcription to obtain cDNA; carrying out PCR amplification on the antibody gene segment and cloning the antibody gene segment to a phage display vector; and electrically transforming the vector into SS320 competent cells to obtain the nano antibody library. After two rounds of panning, detecting and screening an antibody clone capable of being combined with the CD3 protein through ELISA (Enzyme-Linked Immunosorbent Assay); an antibody sequence is selected through sequencing, and an antibody protein is expressed by using a mammalian cell high-throughput expression system; and then detecting and screening the antibody which is only specifically combined with the CD3. The method disclosed by the invention is simple, convenient and rapid to operate, and can be used for preparing active antibodies for treating potential tumors and proliferative diseases.

The invention discloses pressureless sintered aluminum nitride ceramic as well as a preparation method and application thereof. The preparation method comprises the following steps: ball-milling and mixing micron-sized aluminum nitride, a sintering aid, nano-sized molybdenum powder, a dispersing agent, a binder and an organic solvent to prepare slurry; and drying the slurry, granulating, forming, dumping and sintering to obtain the pressureless sintered aluminum nitride ceramic. Wherein the sintering aid is a combination of samarium fluoride and yttrium fluoride. According to the preparation method, oxygen is removed at a low temperature by virtue of strong affinity of the nanoscale molybdenum powder and oxygen, the nanoscale molybdenum powder and the sintering aid jointly play a role in liquid-phase sintering assistance at a high temperature, densified sintering is realized and grain growth is inhibited by adopting pressureless sintering at a relatively low sintering temperature, the aluminum nitride ceramic with both heat conduction and strength is obtained, and meanwhile, a low-resistance second phase is generated; the resistivity of the aluminum nitride ceramic is adjusted while the heat conduction characteristic of the aluminum nitride ceramic is improved, the electrical conductivity of the aluminum nitride material is improved, and finally the aluminum nitride ceramic with high heat conduction and bending strength and moderate resistivity is obtained to meet the use requirements of electrostatic chucks.

The invention belongs to the technical field of immunodetection, and discloses a T3 sandwich method rabbit monoclonal antibody mAb3 and application thereof. The T3 sandwich method monoclonal antibody mAb3 provided by the invention comprises a light chain variable region and a heavy chain variable region, the light chain variable region comprises LCDR1-3, the LCDR1 comprises an amino acid sequence NIYNS, the LCDR2 comprises an amino acid sequence YAS, and the LCDR3 comprises an amino acid sequence QSHYGFSSSYGAA; the heavy chain variable region comprises HCDR1-3, the HCDR1 comprises an amino acid sequence GFDVSYN, the HCDR2 comprises an amino acid sequence IDTGANA, and the HCDR3 comprises an amino acid sequence ARVFYDNSFDP. The monoclonal antibody can be combined with a T3 compound in a high-specificity mode, is suitable for double-antibody sandwich immunodetection and is applied to an immunodetection kit prepared through a double-antibody sandwich ELISA or a chemiluminescence method, a T3 standard antigen is detected through a double-antibody sandwich chemiluminescence platform, the detection sensitivity is lower than 0.3 ng / ml, clinical samples are detected through a magnetochemiluminescence method, and the detection sensitivity is lower than 0.3 ng / ml. In the sample range of 1-80 pmol / L, the correlation with clinical comparison is good.

The invention belongs to the technical field of polypeptides, and discloses a polypeptide targeting HER2 protein, a radioactive polypeptide and a preparation method and application of the radioactive polypeptide, and the structural formula of the radioactive polypeptide is as follows: # imgabs0 #. Through in-vitro radiochemical property research and in-vivo stability research, the 68Ga-DOTA-AON is found to be high in stability and hydrophilic; through cell uptake research and receptor binding force research, the 68Ga-DOTA-AON is found to have relatively high affinity to HER2 protein expressed by HT-29 cells; the tumor imaging effect of the radioactive polypeptide 68Ga-DOTA-AON is investigated through small animal PET / CT, and it is found that [68Ga] Ga-DOTA-AON can be used for in-vivo imaging of HER2 positive colorectal cancer.

The invention discloses a cellulose-based in-situ growth MOF composite aerogel preparation method, which comprises: mixing cellulose powder and dimethylimidazole, adding into deionized water, gradually adding cobalt nitrate and zinc chloride, uniformly stirring, carrying out a heating reaction, adding polyethyleneimine, uniformly stirring, adding a cross-linking agent, carrying out cross-linking, washing, and drying to obtain the cellulose-based in-situ growth MOF composite aerogel. The composite hydrogel is obtained; and finally, sealing, heating and baking the composite hydrogel, washing and drying to obtain the composite aerogel. According to the method, the MOF is synthesized on the cellulose in situ under the low-temperature water phase condition, the composite aerogel containing the MOF material is obtained, the MOF material synthesized on the cellulose in situ has good porosity and particle size and uniformly covers the cellulose, loss of active components is avoided, the problem that the MOF material is prone to agglomeration is solved, and the preparation method is suitable for industrial production. Meanwhile, the adsorption performance is also effectively improved, the adsorbent has good affinity with water, and the surface can be promoted to adsorb three metal ions of vanadium, molybdenum and nickel at the same time.

The invention provides a high-performance nitrogen-containing hard alloy material based on a vanadium-titanium rare earth carbonitride solid solution and a preparation method of the high-performance nitrogen-containing hard alloy material, and belongs to the technical field of hard alloy cutting tool materials. Tungsten carbide of a hard phase is modified, meanwhile, the vanadium-titanium rare earth carbonitride solid solution and the boron quantum dot are introduced to modify the high-entropy alloy, through interaction of modified components, the hardness and transverse breaking strength of the hard alloy material are effectively improved, and meanwhile the phase granularity is reduced.

The invention relates to the field of fertilizers, in particular to a pyroligneous liquor and fulvic acid mixed liquid fertilizer and a preparation method thereof.The pyroligneous liquor and fulvic acid mixed liquid fertilizer is prepared from, by weight, 30-35% of pyroligneous liquor, 25-40% of fulvic acid, 4-6% of amino acid, 5-7% of modified activated carbon and the balance water. The wood vinegar and fulvic acid mixed liquid fertilizer prepared by the invention has good antibacterial performance, and can promote the growth of crops, so that the yield is increased.

The invention discloses preparation of an antibody capable of detecting multi-subtype KPC and application of a kit of the antibody, and belongs to the field of biological medicine. According to the kit for detecting the common subtypes of KPC and the application of the kit, the mouse antibodies are prepared through immunization of conserved antigen epitopes of all the subtypes of KPC, in-vitro screening and pairing are carried out in multiple modes, then an antibody pair which is good in specificity and high in affinity and can be combined with multiple subtypes is obtained, the titer of the two antibodies can reach one million or above, and the kit is high in specificity, good in affinity and capable of being used for detecting the common subtypes of KPC. Amino acid sequences of a heavy chain variable region and a light chain variable region are obtained through sequencing, and rapid and stable expression can be realized after recombination. The product for detecting the carbapenem KPC is developed on the basis of an immunochromatography platform, and the kit has the advantages of high sensitivity, good specificity, good stability, capability of covering most epidemic KPC subtypes and rapidness and convenience in detection, and provides important help for clinical auxiliary diagnosis and medication guidance.

The invention relates to a production process of a high-elasticity heat-resistant polyester yarn, which comprises the following steps: firstly, selecting high-molecular-weight polyethylene glycol terephthalate, adding 1-3% of nano filler and 5-10% of functional copolymer, and drying at 150 DEG C for 4-6 hours to improve the stability and processability of the material; secondly, melt extrusion is conducted at 280-290 DEG C, spinning is conducted through a multi-section temperature control spinning head, and the fiber cooling uniformity is effectively controlled. Then, two times of drafting and a heat setting process are adopted, the orientation degree of a molecular chain is improved in one time of drafting, and low-temperature plasma treatment is combined to optimize the surface activity and the setting effect of the fibers. In the secondary drafting, the fiber deformation is finely adjusted at low temperature, the thermal damage is reduced, the elasticity is improved, and finally, xylanase treatment and ultraviolet curing are combined, so that the softness, the skin-friendly property and the weather resistance of the fiber are remarkably improved.

The invention discloses a porcine reproductive and respiratory syndrome virus N protein antigen epitope peptide and a monoclonal antibody thereof. The amino acid sequence of the antigen epitope peptide is as shown in SEQ ID No. 11; the heavy chain variable region of the monoclonal antibody comprises CDR1-3 as shown in SEQ ID No.1 to SEQ ID No.3; the light chain variable region comprises CDR1-3 as shown in SEQ ID No. 4 to 6; the monoclonal antibody reacts with a variety of PRRSV strains such as classical PRRSV, HP-PRRSV, NADC30PRRSV-like, NADC34PRRSV-like, European PRRSV and the like, and the conservative property is good; the antibody can be obtained by biological means such as conventional genetic engineering, and the problem that the genetic sequence of the antibody is easy to lose due to long-term cryopreservation of traditional hybridoma cells is avoided; optimization of the antibody on the gene or protein level is facilitated, and the specificity and affinity of the antibody are further improved.

The invention provides a quick-charge and quick-discharge type lithium battery and a preparation method thereof, and belongs to the technical field of lithium battery preparation. The preparation process comprises the following steps: preparing a fluorinated carbon nanotube based on biomass carbon; preparing a positive electrode plate; preparing the dendritic mesoporous silicon dioxide nano particles; preparing a cerium oxide-loaded two-dimensional hierarchical porous composite material; preparing a negative electrode plate; and assembling the lithium battery. According to the preparation method, the eutectic and the carbon fluoride nanotubes are added in the preparation of the positive electrode plate, and the cerium oxide-loaded two-dimensional hierarchical porous composite material and the carbon fluoride nanotubes are added in the preparation of the negative electrode plate, so that the prepared positive electrode plate and the prepared negative electrode plate have relatively high conductivity; and moreover, the ion transmission efficiency is improved, the rapid ion transmission is beneficial to reducing the polarization phenomenon of the battery, improving the charging and discharging rate and the power performance of the battery, enhancing the structural stability of the electrode, improving the performance and the safety of the battery, and being more beneficial to rapid charging and rapid discharging.

The invention belongs to the field of biological medicine, and relates to an anti-SEZ6 nano antibody or antigen binding fragment and application. The anti-SEZ6 nano antibody or the antigen binding fragment comprises three complementary determining regions, namely, CDR1, CDR2 and CDR3; the sequences of all the regions are respectively shown as SEQ ID NO: 1 to SEQ ID NO: 18. Compared with a conventional antibody, the anti-SEZ6 nano antibody is efficient in expression, simple in purification, small in molecular weight, high in affinity, relatively good in specificity, high in solubility and strong in tolerance, and can be widely applied to the fields of scientific research, diagnosis and treatment.

The invention discloses a preparation method and application of a manganese-based amino acid coordination polymer, and belongs to the technical field of nano-enzymes and nano-drugs. Divalent manganese salt, histidine and other amino acids are subjected to a co-precipitation reaction to prepare the manganese-based amino acid coordination polymer, and the polymer is of a nanoparticle structure, small in size and high in stability, has enzyme-like activity, catalyzes hydrogen peroxide to generate high-toxicity hydroxyl free radicals and is used for specifically killing tumor cells. The catalyst has the capability of catalytically generating singlet oxygen in the presence of hydrogen peroxide. Meanwhile, the manganese-based amino acid complex has glutathione oxidase-like activity, consumes glutathione to overcome the resistance of tumors to oxidative stress, improves the chemical kinetic curative effect, and has anti-tumor application potential.

The invention relates to an anti-VIM nano antibody and application thereof, belongs to the technical field of biology, and aims to solve the problem of Vimentin VIM detection, and has the key points that a complementary determining region CDR of the nano antibody comprises CDR1, CDR2 and CDR3 sequences: (I): (1) the amino acid sequence of the CDR1 is shown as SEQ ID NO.27, and (2) the amino acid sequence of the CDR2 is shown as SEQ ID NO.33; and (3) the amino acid sequence of the CDR3 is shown as SEQ ID NO.39. The nano antibody and the polypeptide thereof have the effects that the nano antibody and the polypeptide thereof have very high affinity and activity, can specifically recognize and combine with VIM, can be used for capturing and detecting VIM positive cells, and can be applied to immunofluorescence analysis or immunohistochemical analysis and the like through a proper antibody labeling technology.

The invention discloses a non-natural amino acid as well as a preparation method and application thereof. The novel non-natural amino acid is obtained by introducing long and soft side chain amino acid into a classical ASF side chain for the first time, the stability of the obtained non-natural amino acid is enhanced, the reaction radius of the non-natural amino acid is increased, the non-natural amino acid can touch amino acid residues with farther distance, and the reactivity is improved. In addition, compared with other covalent amino acids, the covalent amino acids have higher reaction rate and binding efficiency. The polypeptide and related drugs obtained based on the non-natural amino acid can be high in safety, can cross a blood brain barrier to realize brain targeting, and have better anti-tumor activity, so that the polypeptide and related drugs have extremely high medical value.

The invention discloses preparation and application of an antibody capable of detecting various IMP variants, and belongs to the field of biological medicine. According to the invention, a conserved epitope sequence of IMP enzyme is obtained according to comparison of amino acid sequences of all IMP variants, and an antibody of a spatial epitope is prepared through structural analysis of the epitope. After a mouse is immunized with IMP immunogen protein, hybridoma cells secreting antibodies are obtained through multi-technology platform screening, specific antibody pairs MK35G9 and MK56G2 which are high in affinity and can be combined with multiple IMP variants are obtained after sequencing, and corresponding CDR is obtained through analysis. According to the present invention, the IMP enzyme detection product development is performed based on the immunochromatography platform, such that advantages of high sensitivity, good specificity, covering of a variety of IMP variants, and rapid and convenient detection are provided, and important help is provided for clinical auxiliary diagnosis and medication guidance.

The invention discloses preparation and application of an imido covalent organic framework material containing imidazolyl ionic liquid. The imido covalent organic framework material (ILs-TPN-COFs) containing the imidazolyl ionic liquid is obtained by connecting the imidazolyl ionic liquid [CnCmILs (at) Br] to an imido covalent organic framework TPN-COFs through a covalent bond. The material has an ionic liquid tertiary amine N active site and a halogen anion active site, and can promote CO2 activation and ring opening of epoxide. The ILs-TPN-COFs catalytic material has the advantages of uniform distribution of active sites, better stability, easiness in separation of the catalyst from a reaction system, better repeatability and the like. According to the invention, the prepared novel ILs-TPN-COFs material is applied to catalysis of synthesis of propylene carbonate from carbon dioxide and epoxypropane, and shows excellent catalytic performance under mild reaction conditions.

The invention belongs to the field of sewage treatment, and particularly relates to a preparation method and application of a biological composite carbon source, and the preparation method comprises the following steps: mixing waste beer yeast liquid and biodiesel byproducts, adding an enzyme preparation, carrying out mixing reaction, and separating out supernatant to obtain the biological composite carbon source. The biological composite carbon source disclosed by the invention is low in production cost, rich in nutritional ingredients and high in adaptability, and has long-term effectiveness, high denitrification rate and denitrification effect.

The invention belongs to the technical field of biological pharmacy, and particularly relates to a monoclonal antibody combined with monkey pox virus B6R protein and application of the monoclonal antibody. The antibody is any one or a combination of more of B6R-B5, B6R-F6 and B6R-F11, each of the B6R-B5, the B6R-F6 and the B6R-F11 comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region has three heavy chain CDRs, and the light chain variable region has three light chain CDRs. The invention provides three groups of monoclonal antibodies with excellent affinity with the monkey pox virus B6R and disclosed heavy chain and light chain variable region sequences, provides technical support for preparing a detection and diagnosis reagent of the monkey pox virus, and is expected to be developed into a special product for screening and diagnosing the monkey pox virus. High application values are realized on prevention, control and treatment of the monkey pox virus.

The invention belongs to the field of drug design, and particularly discloses a virtual screening method of a small-molecule inhibitor targeting YTHDF1 protein, which comprises the following steps: acquiring a YTHDF1 protein structure, and carrying out homologous modeling to generate various conformation proteins; small molecules combined with the YTHDF1 protein are obtained, bait molecules are generated, and ligand molecules are formed; a ligand molecule is in butt joint with a binding site on the YTHDF1, and the compound is characterized through PLEC fingerprints; the method comprises the following steps: constructing a specificity scoring function by using a machine learning algorithm, carrying out scoring screening on a compound to be screened and a compound butted with YTHDF1 molecules, carrying out affinity prediction, and screening out the small-molecule inhibitor targeting YTHDF1. According to the virtual screening method provided by the invention, the YTHDF1 targeting molecules with high affinity can be accurately screened on a large scale, candidate molecules are provided for research and development of medicines for treating YTHDF1 related diseases, and a foundation is laid for clinical tests and medicine optimization.

According to the method, phosphomolybdic acid, copper chloride and ferric chloride serve as precursors, copper and iron-containing phosphomolybdic acid nano-enzyme (Cu, Fe-PMs) with peroxidase-like activity is synthesized through a microwave method, under the acidic condition, on one hand, the surface of Cu and Fe-PMs carries negative charges, high affinity is generated between Cu and Fe-PMs and a substrate TMB with positive charges, and on the other hand, the surface of Cu and Fe-PMs has negative charges; on the other hand, Cu / Fe / Mo three-metal redox couple accelerates the regeneration of Fe < 2 + > and plays an important role in the activity of peroxidase, and meanwhile, due to the introduction of sodium dehydroacetate, Cu and Fe in Cu and Fe-PMs and pyranoid ring and keto O in sodium dehydroacetate interact through coordinate bonds to cause electron interaction, so that the peroxidase-like activity of Cu and Fe-PMs is enhanced, and the peroxidase-like activity of Cu and Fe-PMs is improved. And the Fenton reaction is enhanced through transfer of electrons among the sodium dehydroacetate, the Cu and the Fe-PMs. When Cu and Fe-PMs oxidize TMB and H2O2, sodium dehydroacetate enhances the absorbance of the system, and on the basis, a novel colorimetric rapid detection method of sodium dehydroacetate is established.

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention relates to a Nipah virus N protein specific monoclonal antibody as well as a preparation method and application thereof. The nipah virus specific monoclonal antibody is prepared by immunizing nipah virus N protein, the amino acid sequence of the nipah virus N protein is as shown in SEQ ID NO.2, the nipah virus N protein is obtained by transcription of a nipah virus N gene, and the nucleotide sequence of the nipah virus N gene is as shown in SEQ ID NO.1. The nipah virus specific monoclonal antibody has the advantages that the nipah virus specific monoclonal antibody is prepared by immunizing the nipah virus N protein; the monoclonal antibody with the nipah virus N protein specificity can be specifically combined with the nipah virus N protein and is high in titer and good in affinity, meanwhile, a detection kit prepared based on the antibody can effectively carry out nipah virus detection, and technical support can be provided for epidemiological monitoring and diagnosis of nipah virus infection.

The invention discloses an anti-idiotype antibody for detecting and neutralizing an anti-D antibody and application of the anti-idiotype antibody, the anti-idiotype antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises at least one of an HCDR1 sequence, an HCDR2 sequence and an HCDR3 sequence, and the amino acid sequence of the HCDR1 has the amino acid sequence shown as SEQ ID No.9; the amino acid sequence of the HCDR2 has an amino acid sequence as shown in SEQ ID No. 10; the amino acid sequence of the HCDR3 has the amino acid sequence as shown in SEQ ID No. 11. The anti-idiotype antibody can be specifically combined with an anti-D antibody, and can be applied to clinical blood type detection or prevention or treatment of anti-D antibody related diseases.

The invention relates to an anti-CD276 nano antibody. The anti-CD276 nano antibody comprises a complementary determining region (CDR) and a framework region (FR), wherein the complementarity determining region CDR comprises complementarity determining regions CDR1-CDR3, and the complementarity determining regions CDR1-CDR3 are And the framework region FR comprises framework regions FR1-FR5. The anti-human CD276 nano antibody provided by the invention has the characteristic of immunoreaction with a CD276 antigen, is good in specificity and high in affinity, can be applied to preparation of a CD276 detection reagent or an anti-tumor drug and the like, and can be used as an antigen recognition structural domain of a CAR so as to construct a CAR-T cell; the compound has a remarkable killing effect on a plurality of tumor cell lines expressing CD276 antigens; the anti-human CD276 nano antibody provided by the invention can be used for detecting the expression of CD276 in tumor tissues.

The invention discloses a method for preparing tetrakis hydroxymethyl phosphonium sulfate by using high-concentration hydride, and belongs to the technical field of preparation of tetrakis hydroxymethyl phosphonium sulfate. The method for preparing tetrakis hydroxymethyl phosphonium sulfate by using the high-concentration hydride comprises the following steps: step 1, adding concentrated sulfuric acid and formaldehyde into a reaction kettle, uniformly stirring and mixing, then adding an organic catalyst and a modified surfactant, and continuously stirring to completely and uniformly mix reactants to obtain a mixed solution; 2, hydride is introduced into a reaction kettle through a pipeline and reacts with the mixed solution, a heating reaction is conducted, after the reaction is finished, the pipeline is closed, and a reaction solution is obtained; and 3, carrying out vacuum concentration treatment on the reaction liquid, and recrystallizing to obtain tetrakis hydroxymethyl phosphonium sulfate. The tetrakis hydroxymethyl phosphonium sulfate prepared by the method has excellent yield and purity.

The invention provides thiamethoxam water dispersible granules and a production process thereof, and belongs to the technical field of pesticide preparation. The production process comprises the following steps: modifying methyl methacrylate; preparing an anti-ultraviolet carrier; loading eugenol; and preparing the water dispersible granules. The water dispersible granule is prepared by mixing the uvioresistant carrier loaded with eugenol with thiamethoxam, the dispersing agent, the wetting agent, the disintegrating agent and the binder, when eugenol and thiamethoxam are combined for use, the pest killing effect can be synergistically enhanced, and in addition, eugenol can bypass the resistance mechanism of pests to thiamethoxam, so that the pest killing effect is improved. Even if pests have certain tolerance to neurotoxicity of thiamethoxam, eugenol can still play a role by destroying cell membrane structures and respiratory systems of the pests, so that the resistance of the pests is broken, and the overall insecticidal effect of the prepared water dispersible granule is enhanced.

The invention discloses a lithium battery organic-inorganic composite flame-retardant diaphragm which comprises a base material diaphragm and a flame-retardant layer on the outer side of the base material diaphragm, the base material diaphragm is a polyolefin diaphragm, and the flame-retardant layer is prepared from benzoxazine resin particles, natural clay mineral nanotubes and auxiliaries; the mass ratio of the benzoxazine resin particles to the natural clay mineral nanotubes is (40%-95%): (5%-60%). According to the present invention, the design is novel, the thermal stability and the flame retardant property of the polyolefin diaphragm in the lithium battery are effectively improved, the affinity of the polyolefin diaphragm to the electrolyte is improved, and the safety of the battery monomer is substantially improved.

The invention provides application of a compound for regulating and controlling an METTL3 target spot in preparation of an anti-enterovirus drug, and belongs to the field of biological medicine, the compound is AN-465 / 42162872, and the compound has a relatively strong anti-EV71 effect in both in-vitro and in-vivo infection models. An in-vitro cell experiment, EV71 infected C57BL / 6 suckling mouse model, m6A methylation sequencing and other methods are utilized to research the activity and potential action mechanism of anti-EV71, and the result shows that AN-465 / 42162872 can inhibit duplication of EV71 in a human RD cell strain in a dose-dependent manner under the condition of maintaining relatively good cell activity, so that the application of AN-465 / 42162872 in the preparation of the EV71 drug is developed. The action mechanism is mainly as follows: the methylation level of the 5002 adenine in the 2C region of EV71 nucleic acid is remarkably reduced by regulating the METTL3, so that virus replication is inhibited, and the METTL3 has relatively high anti-EV71 drug utilization value.