328results about How to "High affinity" patented technology

The invention provides a hydroxytyrosol supramolecular compound and a preparation method and application thereof, and belongs to the technical field of cosmetics and biological medicine, the supramolecular compound comprises the following chemical components by mass: 0.01-10% of hydroxytyrosol, 2-45% of an amino acid compound, 2-20% of alpha hydroxy acid, 2-30% of urea, and 15-92% of water. The supramolecular compound can promote transdermal delivery of hydroxytyrosol and maintain the biological activity of hydroxytyrosol, shows high biocompatibility, and can be applied to product development in the antibacterial field, the regeneration field and the anti-aging field.

The invention discloses a nano antibody of pre-F protein of a targeted respiratory syncytial virus and application of the nano antibody. The nano antibody comprises a heavy chain variable region, and the heavy chain variable region comprises heavy chain complementary determining regions CDR1, CDR2 and CDR3. The nano antibody disclosed by the invention can be specifically combined with RSV, and has a good application prospect in the aspect of RSV infection related diseases.

This invention discloses a magnetic solid-phase extraction method for detecting trace amounts of glucocorticoids in hydrophilic media, belonging to the field of sample pretreatment and chromatographic analysis technology. The method includes: adjusting the pH of a hydrophilic medium sample containing glucocorticoids and adding an ionic strength modifier to obtain a pretreated sample; mixing a magnetic covalent organic framework-based composite nanomaterial with the pretreated sample and performing oscillatory extraction under a dynamic gradient magnetic field to adsorb the glucocorticoids onto the material surface; separating the material adsorbed with glucocorticoids using an external magnetic field and discarding the supernatant; performing ultrasonic-assisted elution of the material using an elution solvent and collecting the eluent; and detecting the eluent using high-performance liquid chromatography-diode array detector (HPLC-DID) and calculating the glucocorticoid concentration based on a standard curve.

The invention relates to the field of building waterproofing, and discloses a plasticizer and a preparation method thereof, a composition for a polyether waterproof coating, the polyether waterproof coating and a preparation method of the polyether waterproof coating. The method for preparing the modified polyether plasticizer comprises the following steps: (1) in a protective atmosphere, in the presence of a base catalyst, stirring and mixing alpha, omega-dihydroxy polydimethylsiloxane and alkylene oxide to obtain an intermediate I; and (2) contacting and mixing a neutralizer with the intermediate I to obtain the modified polyether plasticizer, relative to 100 parts by weight of the alpha, omega-dihydroxy polydimethylsiloxane, the use amount of the base catalyst is 0.1 to 1 part by weight, the use amount of the alkylene oxide is 400 to 900 parts by weight, and the use amount of the neutralizer is 0.12 to 1.2 parts by weight. The silane modified polyether waterproof coating prepared from the modified polyether plasticizer provided by the invention has obviously better weather resistance.

This invention relates to the field of zinc alloy surface modification technology, and particularly to a corrosion-resistant medical zinc alloy, its preparation method, and its applications. The preparation method includes the following steps: weighing raw materials according to the chemical composition of the zinc alloy; cleaning the surface of the raw materials; smelting, refining, and casting to obtain an ingot; subjecting the ingot to equal-channel angular extrusion deformation to obtain an extruded zinc alloy; performing high-energy ion implantation on the zinc alloy to obtain a nitrogen-ion-implanted zinc alloy; and preparing a coating on the surface of the nitrogen-ion-implanted zinc alloy to obtain the final product. This invention achieves a high-adhesion, dense, and uniform deposition effect on the alloy surface through the synergistic control of "equal-channel angular extrusion + high-energy nitrogen ion implantation + biomimetic coating deposition," solving the problem of severe galvanic corrosion at localized sites in existing zinc alloys under corrosive environments. Simultaneously, the dense deposition layer helps inhibit rapid dissolution of the zinc alloy surface, significantly reducing the corrosion rate of the zinc alloy in corrosive environments.

The application discloses a dihydroquinazolinone-containing N-hydroxy amide compound and a preparation method and application thereof, relates to the technical field of medicinal chemistry, and utilizes the splicing principle to design and synthesize dihydroquinazolinone-containing N-hydroxy amide compounds with novel structures, and the compounds are tested in terms of HDAC6 protein affinity and anti-proliferation activity of cervical cancer cell Siha, and the results show that the compounds E1-E5 have better affinity to the HDAC6 protein, the IC 50 values are 4.1-85.4 nM, wherein the affinity of the compound E4 to the HDAC6 protein is higher than that of a positive control SAHA; and the compounds E1-E5 can better inhibit the proliferation of the cervical cancer cell Siha, the IC 50 values are 2.4-9.3 muM, wherein the activity of the compound E4 is the strongest.

This invention discloses a fusion protein targeting the urokinase-type plasminogen activator receptor and its application in the field of anti-aging. The fusion protein is composed of a hepatocyte growth factor active domain and a humanized anti-uPAR single-domain antibody linked by a flexible linker peptide, and its specific amino acid sequence is SEQ ID NO:3. This fusion protein can specifically bind to uPAR, which is highly expressed on the surface of senescent cells, with intramolar affinity, achieving targeted delivery of the hepatocyte growth factor active domain. In vitro experiments have demonstrated that this protein can significantly reverse the aging phenotype of human fibroblasts, effectively reduce aging-related β-galactosidase activity, p16INK4a protein expression, and interleukin-6 secretion, and promote cell proliferation. In a rapidly aging mouse model, this protein can systematically improve age-related physiological functional decline. The fusion protein of this invention achieves specific targeting and efficient treatment of senescent cells, providing a new solution for the development of anti-aging drugs.

The application belongs to the technical field of natural substance separation and extraction, and particularly relates to a kind of Haematococcus pluvialis multi-product cascade separation method based on eutectic solvent sequential extraction and enzyme membrane reactor coupling, comprising the following steps: (1) cell wall weakening pretreatment step; (2) water-soluble component extraction step; (3) pigment component extraction step; (4) astaxanthin extraction step; (5) enzyme conversion step. Through the synergistic effect of eutectic solvent sequential extraction and enzyme membrane reactor, efficient, green and continuous cascade separation of water-soluble components, pigments, astaxanthin and xylo-oligosaccharides in Haematococcus pluvialis is realized, and product purity and resource utilization are improved.

This invention relates to transition layer materials and welding processes for welding dissimilar steel and aluminum materials, belonging to the field of welding technology. The transition layer material for welding dissimilar steel and aluminum materials of this invention comprises a copper layer, a copper alloy layer, and a zinc alloy layer arranged sequentially; the copper layer is adjacent to the steel base material, and the zinc alloy layer is adjacent to the aluminum base material; the copper content in the copper alloy layer is 62.0%-64.0%; the zinc content in the zinc alloy layer is 89.0%-91.0%; the mass ratio of the copper layer, copper alloy layer, and zinc alloy layer is (0.9-1.1):(2.3-2.7):(1.8-2.2). By sequentially setting the copper layer, copper alloy layer, and zinc alloy layer between the steel base material and the aluminum base material, a gradual transition from the steel base material to the aluminum base material is achieved, effectively avoiding the formation of brittle Fe-Al intermetallic compounds during direct welding, and enhancing the mechanical properties and durability of the welded joint.

This invention relates to the field of polymer materials technology, specifically disclosing an environmentally friendly processing aid for polyolefins and its preparation method. The environmentally friendly processing aid for polyolefins includes a first component and a second component. The first component includes an antioxidant-modified oligomer A, and the second component is obtained by extrusion granulation of oligomer B, an organosilicon polymer, and a catalyst. By weight, the amounts of each component are: oligomer A 1-10 parts, antioxidant 0.1-2 parts, oligomer B 5-40 parts, organosilicon polymer 5-80 parts, and catalyst 0.1-5 parts. The processing aid of this invention does not contain perfluorinated or polyfluoroalkyl compounds. When used in the processing of polyolefins, it has advantages such as low addition amount, short onset time, good viscosity reduction effect, and effective improvement of product gloss. Furthermore, it can effectively form a dynamic lubricating layer on the surface of metal walls.

This invention provides anthracene methylphenthiazide for use in photosensitive resin compositions, relating to the field of photopolymerization. The invention also includes photosensitive resin compositions containing the anthracene methylphenthiazide, photosensitive dry films containing the anthracene methylphenthiazide, and their applications. The anthracene methylphenthiazide of this invention is photosensitizing and can be used as a photosensitizer in photosensitive resin compositions. It also enhances the adhesion of photosensitive resin compositions to various metal surfaces and exhibits low migration characteristics; it can be widely used in photocuring fields such as dry films, paints, coatings, inks, and molding materials.

The application relates to the technical field of organic light-emitting display, and discloses a cyano-substituted aryl ring-connected triazine compound and application thereof. The cyano-substituted aryl ring-connected triazine compound has a structure as shown in formula (I), the compound provided by the application has high bond energy between atoms, good thermal stability, is conducive to solid-state stacking between molecules, is conducive to injection and migration of excitons, can effectively reduce the driving voltage of an organic electroluminescent device when used as an electron transport material, improves the light-emitting efficiency of the organic electroluminescent device, and prolongs the service life of the organic electroluminescent device. Meanwhile, the cyano substitution can adjust the energy level of the compound, the HOMO and LUMO energy levels are deepened, the compound is more matched with adjacent layers, and the device voltage is reduced. The application further provides an organic electroluminescent device and a display device containing the compound of formula (I).

This invention belongs to the field of industrial UV inkjet printing consumables technology, specifically relating to a UV white inkjet ink, its preparation method, and its application. The UV white inkjet ink contains aliphatic polyurethane acrylate, silane-modified epoxy polyester, reactive diluent monomer, photoinitiator, adhesion promoter, white pigment, and optional additives. The silane-modified epoxy polyester is prepared by the following method: esterification of a diol compound with itaconic acid, followed by a mercapto-olefin click reaction of the resulting unsaturated polyester polyol with mercaptosilane, and then a condensation reaction of the resulting silane-modified polyester polyol with halopropylene oxide to obtain the silane-modified epoxy polyester. The UV white inkjet ink provided by this invention has high adhesion to the substrate and is not easily peeled off after handling friction and outdoor exposure.

The application discloses a recombinant humanized antibody for human TGF-beta 1 protein and application thereof, and belongs to the technical field of biological medicines. The antibody or antigen binding fragment for human TGF-beta 1 protein provided by the application is constructed by variable region complementarity determining regions A, B and C of a TGF-beta 1 protein binding region, and the TGF-beta 1 protein binding region is used for inhibiting tumor cells, thereby providing a new direction for anti-tumor. Meanwhile, the antibody has excellent affinity and specificity, and can be used for preparing a TGF-beta 1 protein inhibitor.

This invention belongs to the field of pharmaceutical preparation technology, specifically relating to an HDAC inhibitor based on a tyrosine backbone, its synthesis method, and its application. The invention uses L-tyrosine as the backbone, with its aromatic ring as the Cap region, linked by a flexible alkyl chain via an ether bond, and terminated by an isohydroxamic acid group (ZBG). The tyrosine backbone provides a rigid structure, reducing entropy loss and enhancing hydrophobic interactions with the HDAC surface. By introducing substituents (such as halogens, methyl groups, etc.) onto the tyrosine benzene ring, the affinity with the HDAC active pocket is further optimized. The prepared compound b19 exhibits an HDAC inhibition capacity of 100%. 50 The concentration reached 26.8 nM, exhibiting superior antitumor activity compared to SAHA, especially against solid tumors such as HeLa cells with IC50. 50 It is 0.55 μM.

The invention discloses a self-lubricating PEEK-based composite material and a preparation method thereof, and belongs to the technical field of PEEK composite materials. The self-lubricating PEEK-based composite material is prepared from the following raw materials in parts by mass: 100 parts of polyether-ether-ketone resin, 10 to 15 parts of polyarylene sulfide ketone, 5 to 10 parts of polyarylene sulfide amide, 15 to 25 parts of epoxy group modified MoS2atCO-SiO2 nano material, 4 to 12 parts of modified carbon fiber and 1 to 2 parts of antioxidant. Wherein the modified carbon fiber is obtained by carrying out acylation reaction on carboxylated carbon fiber and then carrying out grafting reaction on the carboxylated carbon fiber and 2-aminodiphenyl sulfide. According to the invention, polyether-ether-ketone, polyarylene sulfide ketone and polyarylene sulfide amide are blended to form a polymer matrix of the PEEK-based composite material, and the epoxy group modified MoS2atCO-SiO2 nano material and the modified carbon fiber are used as lubricating components, so that the composite material has an excellent self-lubricating effect under the combined action.

The invention provides a granulocyte-macrophage colony stimulating factor antibody and application thereof, and belongs to the technical field of biological medicine. A granulocyte-macrophage colony stimulating factor (GM-CSF) antibody or fragment has high affinity, can effectively block GM-CSF / GM-CSF receptor interaction, and has low immunogenicity. The GM-CSF antibody or fragment can be used for preventing and / or treating diseases caused by human GM-CSF or detecting human GM-CSF protein or diagnosing related diseases caused by the human GM-CSF protein.

This invention relates to the field of biotechnology, and discloses a SaCas9 protein ssDNA aptamer, SaCas9-04, and its screening method and applications. The SaCas9-04 aptamer was obtained through six rounds of screening. Library affinity assays showed that the binding ability of the library increased with each screening round, with the sixth-round library exhibiting very strong affinity. Through analysis of high-throughput sequencing results, 96 single clones were selected for testing. Single-concentration screening using SPR was performed, successfully obtaining several single clones with binding values ​​greater than 30 RU, indicating good binding ability. Further concentration gradient KD testing of SaCas9-04 yielded a KD of 739.2 pM.

The invention relates to the technical field of immunodetection, in particular to an anti-TPO antibody or an antigen binding fragment thereof. The anti-TPO antibody provided by the invention has the characteristics of high affinity and good stability, and provides a raw material source with excellent performance for the preparation of a calibration product / a positive control product / a quality control product of a TPO antibody detection kit.

The invention discloses a papermaking dry strength agent preparation method, which comprises: S1, dissolving chitosan in an acidic aqueous solution to form a chitosan glue solution, and removing oxygen; s2, dropwise adding a high-concentration acrylamide solution, a water-phase monomer mixed solution and an initiator solution for graft copolymerization to obtain a branched prepolymer; s3, a cross-linking agent is added for a cross-linking reaction, and a micro-cross-linked structure is formed; primary amino groups on a chitosan chain are terminated, and branched amphoteric chitosan-polyacrylamide dry strength agent mother liquor is obtained; s4, carrying out composite dispersion on the composite material and a nanocellulose material, and diluting to obtain a diluent; and S5, after a paper machine slushing pump and before a pressurized screen, firstly adding an anionic garbage capturing agent for pre-flocculation, then continuously feeding the diluent, and stirring to obtain the product. According to the papermaking dry strength agent prepared by the preparation method disclosed by the invention, the interface bonding strength of the papermaking dry strength agent to fibers and the adaptability and use efficiency of the papermaking dry strength agent in a complex wet end environment are remarkably improved, and the requirements of the modern papermaking industry on high-performance and high-stability dry strength agents can be met.

The invention belongs to the technical field of talcum powder. The invention provides high-dispersity talcum powder and a preparation method thereof.The preparation method comprises the following steps that S1, zirconium oxide beads are adopted as a grinding medium, the grinding medium and grinding materials are added into a charging barrel of a vertical sand mill, the grinding materials comprise talcum powder raw materials, carboxymethyl cellulose graft copolymers and sodium hexametaphosphate, the rotating speed is adjusted to be 9.5 m / s, the grinding time is 3-12 h, and a mixture is obtained; grinding to obtain ground powder; s2, adding an aqueous dispersion into the ground powder obtained in the S1, and adjusting the rotating speed to 9.5 m / s and the grinding time to 8-36 hours to obtain ground slurry; and S3, drying and crushing the ground slurry obtained in the S1 to obtain the high-dispersity talcum powder. The carboxymethyl cellulose grafted copolymer is added into the grinding material, so that the viscosity of grinding slurry is reduced, and meanwhile, the dispersity of talcum powder particles is improved.

The present application relates to the technical field of biological medicine, in particular to an anti-CEACAM5 antibody and application thereof, the anti-CEACAM5 antibody has the following technical features: the heavy chain variable region includes CDR-H1 with the amino acid sequence shown in SEQ ID No.1, CDR-H2 with the amino acid sequence shown in SEQ ID No.2 and CDR-H3 with the amino acid sequence shown in SEQ ID No.3.The anti-CEACAM5 antibody has high affinity, is used as an antigen binding domain to construct a chimeric antigen receptor, and utilizes the chimeric antigen receptor to prepare a CAR-T cell, the CAR-T cell has killing activity on tumor cells positive for CEACAM5, and after co-culturing with CEACAM5-positive cells, the CAR-T cell can efficiently secrete the cytokine IFN-gamma, the nanobody can be effectively applied to immunotherapy, and has important significance for developing tumor treatment drugs.

This invention discloses an APLN monoclonal antibody, its preparation method, and its applications. The APLN monoclonal antibody includes a light chain variable region and a heavy chain variable region. The amino acid sequence of the light chain variable region is shown in SEQ ID No:1, and the amino acid sequence of the heavy chain variable region is shown in SEQ ID No:2. The APLN monoclonal antibody is obtained by immunizing mice with APLN protein, followed by single-cell B-cell sorting and single-cell PCR. Utilizing advanced single-cell B-cell cloning technology combined with single-cell PCR, a novel APLN monoclonal antibody has been developed. The obtained antibody sequence is the result of natural affinity maturation screening by the in vivo immune system, requiring no additional humanization or in vitro affinity maturation steps. It retains the natural high affinity and specificity, and can directly obtain all-natural antibodies with high affinity and high specificity.

This invention discloses nanobodies against rabies virus G protein and their applications, belonging to the field of nanobody technology. The nanobodies are antibody 11G6, antibody 9F7, or antibody 9A3; the amino acid sequence of antibody 11G6 is shown in SEQ ID NO. 8; the amino acid sequence of antibody 9F7 is shown in SEQ ID NO. 13; and the amino acid sequence of antibody 9A3 is shown in SEQ ID NO. 18. This invention uses phage display technology to screen for nanobodies with high affinity and high neutralizing activity. Based on this, this invention utilizes the advantage of the small size of nanobodies and verifies their unique ability to cross the blood-brain barrier (BBB) ​​through Transwell experiments, overcoming the limitation that large molecules such as antibodies and drugs cannot cross the BBB to enter the brain. This invention provides a new drug option for the treatment of rabies virus and has significant clinical implications.

This invention belongs to the field of molecular biology and medical detection technology, and discloses a bladder cancer-specific nucleic acid aptamer, nucleic acid aptamer derivatives, and their applications. The nucleotide sequences of the nucleic acid aptamer are shown in SEQ ID NO: 1, 2, and 3. This invention screened three monoclonal nucleic acid aptamer DNA chains from bladder cancer tissue samples using the exponential enrichment systematic evolution (SELEX) technique and identified two target proteins that bind to these three nucleic acid aptamers. The nucleic acid aptamers specifically target the target proteins CAPRIN1 and FXR1 on the surface of bladder tumor cells. The nucleic acid aptamers obtained by this invention have advantages such as high affinity and specificity, no immunogenicity, simple chemical synthesis, stability, and ease of storage and labeling, providing a new molecular tool for the early diagnosis and targeted therapy of bladder cancer.

The invention relates to the technical field of aerogel microsphere preparation, in particular to a method for preparing composite nanocellulose aerogel microspheres based on electrostatic field auxiliary regulation and control. Comprising the following steps: forming uniform liquid drops from a silane modified nano cellulose suspension through an electrostatic spraying device provided with an annular electrode group and a real-time monitoring feedback system; the liquid drops are induced by an electrostatic field in a pre-cooling area to be initially set and then subjected to liquid nitrogen cryogenic treatment, gradient freeze drying is conducted, and aerogel microspheres are obtained; and finally, carrying out surface modification on the aerogel microspheres, and loading nano microspheres under the assistance of an alternating electric field to obtain the composite nano cellulose aerogel microspheres. Through the synergistic effect of the multi-stage electric field, accurate regulation and control on the whole process of microsphere forming, internal structure construction and functional compounding are realized, so that the composite nanocellulose aerogel microspheres with narrow particle size distribution, uniform size and adjustable functions are prepared, and the composite nanocellulose aerogel microspheres have wide application prospects in the fields of adsorption, catalysis, biological medicine and the like.

The application provides a method for constructing a double-signal sensing platform based on a non-copper nanometer enzyme AuPt@PBA@L-Pd and application, and a multifunctional nanometer composite material AuPt@L-Pd@PBA (hereinafter referred to as APL) with the effects of an oxidase-like enzyme, a peroxidase-like enzyme and a laccase-like enzyme is prepared. The composite nanometer enzyme has a regular cubic morphology, takes PBA as a base material, loads L-Pd through an amide bond, simultaneously collects the advantages of the MOF material PBA and L-Pd, has multiple enzyme-like effects, and can catalyze different chromogenic substrates. The AuNPs and PtNPs on the surface of APL not only provide good binding sites for antibodies, but also successfully prepare a biological composite probe Ab2@APL, further amplify the sensing signal, and improve the sensitivity of the sensing platform. It is worth mentioning that APL has the effect of a laccase-like enzyme, but does not contain copper ions, and further improves the biocompatibility. Based on the high catalytic activity and multifunctional nanometer enzyme Ab2@APL, a multi-signal sensing platform can be constructed, and the application prospect in more fields is wider.

This invention discloses a feline measles virus-specific monoclonal antibody and its applications. The monoclonal antibody is prepared by immunization with the feline measles virus N protein. The monoclonal antibody specifically recognizes the feline measles virus N protein, which includes the feline measles virus N protein-N fragment with the nucleic acid sequence shown in SEQ ID NO:1 and the feline measles virus N protein-C fragment with the nucleic acid sequence shown in SEQ ID NO:2. The monoclonal antibody provided by this invention can specifically recognize the feline measles virus N protein, exhibiting high titer, good affinity, and high sensitivity. Detection kits prepared based on this antibody can effectively detect feline measles virus in feline blood, urine, and other bodily fluids, providing technical support for the epidemiological monitoring and diagnosis of feline measles virus infection.

This invention provides an endoplasmic reticulum-targeted single-chain cyclic poly(β-amino ester), its preparation method, and its applications. The structural formula of the endoplasmic reticulum-targeted single-chain cyclic poly(β-amino ester) is as follows. The endoplasmic reticulum-targeted single-chain cyclic poly(β-amino ester) exhibits excellent affinity for DNA, and the complex nanoparticles formed with DNA are uniformly distributed, stable, biodegradable, and biocompatible. The endoplasmic reticulum-targeted single-chain cyclic poly(β-amino ester) and DNA can be efficiently taken up by cells, exhibiting excellent endoplasmic reticulum targeting performance. It can overcome the limitations of gene retention in lysosomes and nuclear internalization of the complex nanoparticles, enabling efficient delivery of DNA and mRNA.