175 results about "Streptavidin" patented technology

The invention belongs to the field of immunological detection, and provides a double-antibody sandwich ELISA kit for detecting the content of human beta-NGF. The enzyme-linked immunosorbent assay kit constructed by the invention is based on a double-antibody sandwich method, and is combined with a biotin-streptavidin signal amplification system and an enzyme developing amplification system, so that the detection sensitivity and specificity are greatly improved, and low-content samples can be accurately and quickly detected. The kit shows excellent stability and specificity and can be used for quantitatively detecting the content of beta-NGF in samples such as human serum, cell supernatant or tissue homogenate, so that an objective detection basis is provided for diagnosis, curative effect evaluation and pathogenesis research of related diseases.

The application provides a preparation method of a functionalized phage probe and application of the functionalized phage probe in detection of escherichia coli. The functionalized phage is a recombinant phage (M13KO7@SaBP) in which streptavidin binding peptide (SaBP) is fused and expressed at the N terminal of the main capsid protein P8 protein of M13K07 phage. In the application, the gene encoding SaBP (MDVEAWLGAR) is inserted into the N terminal of the P8 protein gene of M13K07 phage by site-directed mutagenesis to prepare M13K07@SaBP. The stable signal amplification is realized by using the abundant P8 protein (about 2700) of M13 phage and the super strong binding force of biotin-streptavidin, the interference of food sample matrix is reduced by combining the magnetic separation technology, and a detection method of escherichia coli with high sensitivity and small sample matrix interference is constructed.

This invention provides a double-antibody sandwich SA-ELISA detection kit for Fasciola hepatica infection in sheep. A double-antibody sandwich SA-ELISA method for detecting Fasciola hepatica CAg (cag) is established using mouse anti-Fasciola hepatica D38 monoclonal antibody and rabbit anti-Fasciola hepatica D38 polyclonal antibody. This detection uses monoclonal antibodies as coating antibodies, resulting in high specificity. Furthermore, the introduction of a biotin-streptavidin signal amplification system enhances sensitivity. It offers advantages such as high accuracy, good stability, simple operation, rapid detection, and easy interpretation, making it suitable for rapid clinical diagnosis and epidemiological surveys at the grassroots level.

This invention discloses a homogeneous microsphere based on singlet oxygen self-circulating aggregation-induced emission, its preparation, and a wash-free detection method, which improves the sensitivity and efficiency of wash-free luminescent oxygen channel immunoassay. This invention successfully prepared AIE receptor microspheres by embedding the photosensitizer AIER dye, ultra-bright AIEG dye, and singlet oxygen-responsive dye SO into carboxyl-modified polystyrene nanoparticles. Simultaneously, an antibody-1-labeled AIE receptor microsphere solution, an antigen-containing test solution, and biotinylated antibody-2 were added to a 96-well plate, followed by streptavidin-modified donor microspheres. After wash-free incubation, the fluorescence signal value was measured, and the concentration of antigen in the test solution was obtained according to a standard curve. This platform completes wash-free, highly sensitive detection within 5 minutes, innovatively combining the aggregation-induced emission characteristics of AIEgens and a singlet oxygen cycling strategy, providing an efficient and universal solution for wash-free diagnostics.

The invention relates to the technical field of kits, in particular to a magnetic particle chemiluminiscence detection kit for insulin and a preparation method of the magnetic particle chemiluminiscence detection kit for insulin, specifically, a reagent M, a reagent R2 and a reagent R1 are used for forming the kit, and the reagent M is a magnetic particle working solution containing streptavidin magnetic beads; the reagent R2 is an enzyme-labeled working solution containing an insulin antibody labeled by alkaline phosphatase; the reagent R1 is a working solution containing a biotin-labeled insulin monoclonal antibody. Through verification, according to the chemiluminescence kit for insulin provided by the invention, the test sensitivity reaches 2.00 mu IU / mL, the test repeatability for high and low value samples is less than 3%, and the linear correlation with Rogowski reaches 0.98 within the concentration range of 2.00-450 mu IU / mL. The invention also provides a preparation method of the insulin chemiluminescence kit, the production cycle is greatly shortened, the batch difference is easy to control, and the production work of enterprises is facilitated.

Disclosed herein are chromatographic materials comprising crosslinked streptavidin molecules. The crosslinked streptavidin chromatographic materials may be used to prepare affinity chromatographic materials using biotinylated affinity agents, such as a biotinylated antibody, biotinylated antigen-binding fragment, or biotinylated oligonucleotide. Due to the crosslinked nature of the streptavidin molecules, the materials provided herein have reduced to no streptavidin leachate, resulting in improved performance.

The invention discloses a biosensor based on an ultraviolet light triggered bio-barcode release technology (OBCA) and construction and application of the biosensor. The biosensor comprises nucleic acid functionalized silver nanoparticles, nucleic acid functionalized magnetic beads and a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas12a detection system, the DNA bar code modified on the surface of the silver nanoparticle contains a light cleavable linker, a CRISPR / Cas12a activation sequence and a target capture sequence I, and a DNA capture probe containing a target capture sequence II is fixed on the surface of the magnetic bead through biotin-streptavidin action. During detection, target miRNA mediates two capture sequences to form a sandwich compound, after magnetic separation and thermal denaturation elution, 365nm ultraviolet radiation releases DNA bar code fragments, and the Cas12a trans-cleavage fluorescence quenching reporter probe is activated to generate a signal. According to the method, nucleic acid amplification and reverse transcription are not needed, the detection limits of miR-21, miR-155 and mi-210 respectively reach 158.94 aM, 763.56 aM and 197.82 aM, single-base mismatch and precursor miRNA can be distinguished, the serum sample detection result is highly consistent with that of RT-PCR, and the method is suitable for high-sensitivity quantitative detection of miRNA.

The invention discloses a dual-mode detection kit for highly sensitive detection of ERalpha based on HRP-streptavidin. The dual-mode detection kit comprises the following raw materials: a glassy carbon electrode, 1-AP-CNHs, AuNPs, Sh-Apt, MCH, Bio-Ab, and HRP-streptavidin. The invention successfully constructs an electrochemical detection kit for detecting ERalpha with high sensitivity on the basis of the 1-AP-CNHs / AuNPs composite material. According to the invention, due to the redox activity of HRP-streptavidin, the HRP-streptavidin and H2O2 are subjected to redox reaction to generate a current signal; in addition, hydrogen peroxide can be catalyzed to generate. OH free radicals, and the free radicals can oxidize TMB to generate blue products, so that colorimetric reaction is initiated, and ERalpha can be detected more accurately. The electrochemical-colorimetric dual mode of the 3D 1-AP-CNHs / AuNPs composite material and the HRP-streptavidin for high-sensitivity detection of the ERalpha disclosed by the invention greatly improves the detection accuracy. The kit has the advantages of good selectivity, strong stability, high sensitivity, anti-interference performance, environmental protection and the like.

The application provides a skin interstitial fluid miRNA multicolor visualization semi-quantitative detection method, which comprises the following steps: incubating a skin interstitial fluid sample with magnetic nano probes of surface immobilized hairpin probes H1, opening H1 and triggering a subsequent hybridization chain reaction of hairpin probes H2 and H3 in the presence of target miRNA, and constructing a biotin-rich DNA complex on the surface of the magnetic beads; performing enzyme labeling by using an alkaline phosphatase-streptavidin coupling compound, and catalyzing ascorbic acid phosphate to generate ascorbic acid potassium iodate, so that the color of the solution changes, and high-sensitivity detection of the target miRNA is realized. The multicolor visualization detection method can produce near-full-spectrum color changes by combining the gold nano double-cone local surface plasmon resonance property change, and is beneficial to improving the portability of the sensor and the accuracy of the visual analysis.

The invention belongs to the technical field of microbial nucleic acid detection and bioluminescence analysis, and discloses a bioluminescence nucleic acid detection method and kit based on DNA-RNA hybrid specific recognition. The kit comprises a streptavidin coated solid phase carrier, a biotinylated DNA capture probe, a signal enhancement DNA, a luminescence report fusion protein and a luminescence substrate thereof, the luminescent reporter fusion protein comprises a DNA-RNA hybrid binding domain and a luminescent enzyme domain. According to the method, target RNA is captured through a solid phase, a DNA-RNA hybrid is formed, the luminous report fusion protein is specifically combined with the DNA-RNA hybrid, a bioluminescence signal is generated after a luminous substrate is added, and the target RNA is detected. In the embodiment, an amplification step is not adopted, so that the method has the advantages of simplicity and convenience in operation, rapidness in detection, lower background and better specificity, and can be used for rapid detection of microorganisms.

The invention discloses a cryptococcus capsular polysaccharide detection kit. The cryptococcus capsular polysaccharide detection kit comprises a biotin-labeled cryptococcus monoclonal antibody and an acridinium ester-labeled cryptococcus monoclonal antibody, wherein the biotin-labeled cryptococcus monoclonal antibody and the acridinium ester-labeled cryptococcus monoclonal antibody are coupled with streptavidin magnetic beads. According to the cryptococcus capsular polysaccharide detection kit disclosed by the invention, the biotin-labeled cryptococcus monoclonal antibody coupled with the streptavidin magnetic beads and the acridinium ester-labeled cryptococcus monoclonal antibody are matched, so that the cryptococcus capsular polysaccharide detection kit has relatively strong recognition capability on various serotypes of cryptococcus, and the antibodies do not interfere with one another; according to the cryptococcus capsular polysaccharide detection kit, the detection sensitivity and the detection accuracy can be greatly improved, the problem that a detection result is greatly influenced by subjective consciousness and is inaccurate due to the fact that an existing detection method only depends on visual inspection is solved, and the cryptococcus capsular polysaccharide detection kit is convenient to operate, facilitates automatic operation of an instrument and reduces manual errors.

The invention provides a novel enriched photo-crosslinking agent, a preparation method of the novel enriched photo-crosslinking agent and application of the novel enriched photo-crosslinking agent in protein conformation and protein interaction analysis. The cross-linking agent can be subjected to a cross-linking reaction with protein at the moment of illumination, a cross-linked product can be enriched through click chemistry-biotin streptavidin, then steric hindrance of the cross-linked product is reduced by cutting azido biotin groups, and the mass spectrometric detection sensitivity and specificity of the cross-linked product are effectively improved.

The invention relates to ribozyme for labeling biotin on target RNA (Ribonucleic Acid) as well as a screening method and application thereof. The RNA labeling technology is one of core tools for modern molecular biology research and clinical diagnosis. A specific marker is covalently linked to a target RNA molecule, so that accurate tracking, efficient detection and functional analysis of RNA can be realized. A biotin-streptavidin system becomes one of the technical routes which are most widely applied due to extremely high affinity and signal amplification capability of the biotin-streptavidin system. At present, common biotin-labeled RNA technical methods mainly comprise a chemical solid-phase synthesis method, an enzymatic method and a chemical labeling method. However, the methods have the limitations of poor site specificity, low efficiency, complex manipulation and the like in the use process. The engineering ribozyme for catalyzing RNA biotinylation is obtained by taking 12: 0 biotin coenzyme A as a biotin donor through an in-vitro screening technology. The ribozyme provided by the invention can efficiently and specifically carry out biotin labeling on target RNA, the reaction process is simple, and the labeling activity is up to 90% or above.

The invention discloses an engineered exosome preparation with tumor targeting and specific gripper response release functions as well as a preparation method and application of the engineered exosome preparation. The preparation comprises a myocardial cell exosome, an NK cell biotinylation activation antibody, a tumor disease biotinylation antibody, streptavidin, a tumor fiber matrix FAP targeting peptide and a tumor matrix enzyme response peptide, and anchoring is carried out through an acetamide compound-polyethylene glycol anchor point platform. The engineering bispecific exosome preparation with good tumor targeting and barrier breakthrough ability and immune cell activation effect is prepared, and the preparation breaks a solid tumor compact fiber barrier and increases NK cell infiltration by utilizing the fibrosis treatment ability of the myocardial cell exosome and the capture and targeting effects of an anchor point platform; the natural killer cells are in close contact with tumor cells, the lasting tumor cell killing effect is achieved, and the tumor matrix environment is fundamentally changed.

The invention relates to the technical field of digital PCR detection, in particular to a single-molecule protein detection method based on digital PCR. The detection method comprises the following steps: fusing digital PCR and immune PCR, capturing an antibody through magnetic bead coupling, binding the captured antigen with a detection antibody of a nucleic acid fragment through streptavidin-biotin coupling to form a double-antibody sandwich structure, amplifying a fluorescence signal by using digital PCR amplification, and calculating to obtain the target protein concentration of a sample to be detected. Therefore, quantitative detection of the single-molecule protein is realized, and the detection limit reaches 0.395 fg / ml. The detection method is accurate in quantification and excellent in sensitivity, the detection cost can be greatly reduced, the detection efficiency is improved, the detection process is simplified, and the high requirement for equipment precision is reduced.

The application provides a quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids and an application thereof, and belongs to the technical field of veterinary biological diagnosis products. The quantum dot microsphere fluorescent immunochromatography test paper for combined detection of ASFV antibodies and nucleic acids comprises a base plate, on the base plate, a sample pad, a combination pad, a nitrocellulose membrane and a water absorption pad are sequentially connected in a head-to-tail mode, from the combination pad to the water absorption pad, the nitrocellulose membrane is sequentially provided with an antibody detection line, a nucleic acid detection line and a quality control line, the antibody detection line is coated with a goat anti-pig IgG-Fc antibody, the nucleic acid detection line is coated with streptavidin, and the quality control line is coated with a rabbit anti-sheep IgG antibody; the combination pad is coated with a quantum dot microsphere labeled recombinant protein P22 and a quantum dot microsphere labeled sheep anti-digoxin antibody. The test paper can rapidly, highly specifically and highly sensitively detect ASFV antibodies and nucleic acids.

The invention relates to a rapid detection method for cadmium residual quantity in aquatic products based on magnetic qualitative separation and polarized light distinguishing and quantifying. The method comprises the following steps: coupling a biotinylated cadmium monoclonal antibody through streptavidin magnetic microspheres, and specifically enriching trace cadmium in the aquatic product digestive juice to form a magnetic compound; a muller matrix microscope is used for collecting a polarization microscopic image of a compound, and a deep learning model (such as RESNET34) is combined to carry out binary classification and particle counting on the image, so that rapid and high-sensitivity quantitative detection of cadmium can be realized. The method is easy and convenient to operate, the target object magnetic separation time is within 2 min, the particle distinguishing accuracy rate is higher than 84%, the quantitative correlation Rreaches 0.9 or above, and the method is suitable for rapid monitoring of trace cadmium (several micrograms / L) in aquatic products.

The invention discloses a dermatophyte nucleic acid rapid detection kit, which comprises: a CRISPR / Cas12a reaction system, which comprises a Cas12a protein and a corresponding crRNA; the fluorescent reporter probe is provided with a 5 '-end FAM label and a 3'-end biotin label; an anti-FAM antibody-fluorescent quantum dot conjugate; the quantum dot immunochromatography test strip comprises a sample pad, a nitrocellulose membrane and an absorption pad, and the nitrocellulose membrane is provided with a test line and a control line; the control line is coated with streptavidin, and the test line is coated with goat anti-mouse IgG. The invention further discloses a corresponding detection method. Detection can be completed within 1 h, operation is easy and convenient, detection sensitivity and specificity are high, and the method is suitable for rapid detection and field application of dermatophyte.

This invention discloses a method for preparing a copper-doped NiFe PBA nanozyme probe and its application, comprising (1) preparing a copper-doped NiFe PBA nanozyme and connecting it with a secondary antibody Ab2 to obtain a Cu-NiFe PBA-Ab2 nanozyme probe; (2) dropping the antigen molecule and the Cu-NiFe PBA-Ab2 nanozyme probe onto the surface of an epoxy-activated carrier sheet modified with a primary antibody Ab1 and streptavidin, and incubating to form a chemiluminescent imaging immunosensor with a double antibody sandwich core, thereby achieving highly sensitive detection of antigen molecules with a detection limit down to the femtogram level.

The invention relates to the field of single-molecule gene sequencing, in particular to a composite nucleotide probe based on a nucleic acid nanostructure and application of the composite nucleotide probe. The structure of the composite fluorescent nucleotide probe provided by the invention is a multi-level structure, the multi-level structure respectively comprises a nano nucleic acid molecule, streptavidin and a biotin-nucleoside polyphosphate compound from inside to outside, and the nano nucleic acid molecule, the streptavidin and the biotin-nucleoside polyphosphate compound are connected through biotin. The nucleic acid chain, biotin modification and fluorescent dye molecule modification assembled into the nucleic acid nanostructure can be ordered and synthesized by commercial synthesis companies, the price is low, and synthesis is rapid and convenient; the three-dimensional branched connecting molecules can fully disperse the dendritic macromolecules with large volumes in a three-dimensional space. The planar branched connecting molecule is used for connecting the nucleoside polyphosphate and the polyethylene glycol dispersing element, so that the distance between the nucleoside polyphosphate and the polyethylene glycol is relatively short, the polyethylene glycol can conveniently separate and protect the nucleoside polyphosphate, and the diffusion effect of the nucleoside polyphosphate in an aqueous solution is improved.

This application discloses a chemiluminescence detection kit for acrylonitrile magnetic microparticles and its detection method. The kit includes a magnetic bead working solution and a luminescent label. The magnetic bead working solution includes acrylonitrile monoclonal antibody A and streptavidin. The luminescent label includes acrylonitrile monoclonal antibody B, modified nano-silica, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N,N-dimethylformamide, acrylonitrile succinimide ester, glutaraldehyde, and ethanolamine. This application uses modified nano-silica as a carrier and grafts a polyethylene glycol hydrophilic layer onto its surface, which amplifies the acrylonitrile ester molecule signal, reduces non-specific adsorption, and improves the sensitivity, specificity, and reliability of the detection.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention is applicable to the technical field of immunodetection analysis, and provides a thromboregulatory protein quantitative detection kit and a preparation method thereof.The kit comprises a magnetic bead working solution, a biotin marker working solution, an acridinium ester marker working solution, a thromboregulatory protein calibration product and a thromboregulatory protein control product; the magnetic bead working solution is composed of streptavidin magnetic beads and a magnetic bead diluent, the biotin marker working solution is composed of a biotin-labeled thromboregulatory protein antibody and a biotin diluent, and the acridinium ester marker working solution is composed of an acridinium ester-labeled thromboregulatory protein antibody and an acridinium ester diluent. The thromboregulatory protein quantitative detection kit provided by the invention integrates high sensitivity, high specificity, high precision, strong anti-interference capability, wide linear range and excellent stability, and provides an ideal solution for realizing accurate, rapid and automatic quantitative detection of thromboregulatory protein.

The invention discloses a magnetic particle chemiluminescence detection kit and a detection method thereof, the kit comprises a magnetic bead working solution and a luminescent marker, the magnetic bead working solution comprises a peptin monoclonal antibody A and streptavidin, the luminous marker is prepared from a peptin monoclonal antibody B, modified nano silicon dioxide, anhydrous toluene, methoxy polyethylene glycol-succinimide ester, N, N-dimethylformamide, acridine succinimide ester, glutaraldehyde and ethanolamine. The acridinium ester molecular signal can be amplified by taking the modified nano silicon dioxide as a carrier and grafting the polyethylene glycol hydrophilic layer on the surface, the non-specific adsorption can be reduced, and the effects of improving the sensitivity, specificity and reliability of detection are achieved.

The invention relates to the technical field of medical engineering, in particular to an NK cell delivery data processing method and system based on magnetic targeting. The method comprises the following steps: inquiring an immune receptor protein structure of natural killer cells; injecting a sodium chloride solution, adding an immune receptor protein structure, and reacting to generate an aptamer structure; the method comprises the following steps: combining streptavidin in a pre-prepared glycosyl magnetic microsphere to obtain a high-affinity magnetic bead; carrying out molecular docking on the high-affinity magnetic beads and the aptamer structure, recording a docking score, and generating an optimized aptamer; recording the magnetic bead mass ratio of the optimized aptamer; capturing the natural killer cells by adopting the optimized aptamer, and shooting to obtain a fluorescent microscopic image; and recording the dyeing condition of the fluorescent microscopic image, determining the survival state of the NK cells, marking the position information of the living cells, and generating an NK cell delivery efficiency table by using the mass ratio of the magnetic beads. Based on a medical engineering technology, the homing rate and the delivery efficiency of the NK cells at the liver injury part are improved.

The invention belongs to the technical field of plant gene expression and editing, and discloses composite lipid nanoparticles for plant cell gene delivery as well as a preparation method and application of the composite lipid nanoparticles. According to the composite lipid nanoparticle provided by the invention, the lipid nanoparticle is used as a carrier for loading an active substance, meanwhile, a hydrolase composition is introduced to the surface of the lipid nanoparticle through the strong non-covalent acting force between the streptavidin and the biotin, and the hydrolase composition is introduced to the surface of the lipid nanoparticle through the strong non-covalent acting force between the streptavidin and the biotin; the composite lipid nanoparticles have excellent delivery efficiency on plant cells under the conditions that the plant cell structure is not damaged and the metabolic state of the plant cells and the physiological state of the plant are not influenced, and have very excellent application prospects in the plant breeding technology.

The present application relates to the field of micro-nano magnetic material for in vitro detection, and particularly relates to a preparation method of high-loading streptavidin magnetic microspheres, the magnetic microspheres and application thereof; in the preparation process, first, streptavidin is reacted with a crosslinking agent to obtain polymerized streptavidin, and then the polymerized streptavidin is coupled on the surface of the magnetic microspheres to obtain streptavidin magnetic microspheres; wherein the structure of the crosslinking agent comprises a hydrophilic flexible chain. The present application has the effects of improving the loading and stability of streptavidin on the surface of the magnetic microspheres, reducing non-specific adsorption, and enhancing the sensitivity of detection.

The application discloses a kit for determining anti-sperm antibody IgG and application thereof, and comprises sperm membrane antigen coated carboxyl magnetic beads, an analysis buffer and an enzyme working solution, wherein the sperm membrane antigen coated carboxyl magnetic beads are labeled with biotin, streptavidin and streptavidin antibody; in the application, the streptavidin antibody is added in the coating process of the carboxyl magnetic beads, a carboxyl magnetic bead-streptavidin antibody-streptavidin-biotinized antigen is formed, the connecting length between the antigen and the magnetic beads is increased, the steric hindrance is reduced, and the interference of some sample detection is avoided.

The invention provides a biotinylated Angiopep-2 modified targeting nano material as well as a preparation method and application of the biotinylated Angiopep-2 modified targeting nano material. According to the preparation method of the biotinylated Angiopep-2 modified targeting nano-material, Angiopep-2 is directionally anchored through a high-affinity biotin-streptavidin system, ligand conformation inactivation caused by chemical coupling is avoided, targeting activity is maintained, the used Angiopep-2 can help nanoparticles to be specifically recognized and combined and mediate the nanoparticles to cross a blood brain barrier to enter the brain, and the targeting nano-material can be used for preparing the targeted nano-material. The drug concentration in brain tissues is increased, so that the cure rate of brain diseases is improved, the brain targeting efficiency is far higher than that of transferrin and lactoferrin, and the application value is great; by entrapping curcumin and quercetin, a hydrophilic shell is provided, so that the water solubility of curcumin and quercetin is improved, and the bioavailability of curcumin and quercetin is improved.

The invention relates to a tracer compound for whole-cell labelling, comprising: a non-streptavidin- binding biotin analogue, at least one connecting moiety comprising a functional group configured for a click-chemistry reaction, and at least one cross-linkable moiety, preferably wherein the cross-linkable moiety is a (para)formaldehyde cross-linkable group, and the non-streptavidin-binding biotin analogue enables the tracer compound to diffuse within a biological cell with essentially the same properties as biocytin and / or neurobiotin, preferably enabling the detection of cellular protrusions, such as dendrites, dendritic spines and axons. The invention further relates to methods for constructing such tracer compound and for labelling a cell therewith. The invention further relates to kits for carrying out the method according to the invention.