55 results about "Electrochemiluminescence" patented technology

The application relates to a sensor for synchronously detecting enniatin and roquefortine based on a double-potential electrochemiluminescence immunoassay technology as well as a preparation method and a detection method thereof. The sensor comprises a base electrode, a sensing interface composed of a nanocomposite is modified on the surface of the base electrode, mixed coated antigens including ENNs-BSA and AOH-BSA fixed on the sensing interface, mixed probes including a cathode probe and an anode probe, the cathode probe is Zr-Por-MOF-ENNs Ab which is obtained by marking ENNs antibodies with a porphyrin zirconium metal organic framework, the anode probe is Ce-In-MOF@ABEI-AOH Ab which is obtained by marking AOH antibodies with ABEI loaded on a cerium-doped indium metal organic framework, and a detection solution containing a single co-reactant potassium persulfate. The application can realize one-time sample adding, double-potential scanning and synchronous quantification for two kinds of mycotoxins of enniatin and roquefortine.

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

This invention relates to a dual-potential electrochemiluminescence immunosensor based on enzymatic in-situ generation of a co-reactant, its preparation method, and its application. The sensor includes: a substrate electrode; a GOx@Au@MoS2 multifunctional sensing interface; a hybrid probe consisting of a cathode probe and an anode probe; the cathode probe is a Py-HOF-labeled AFB1 antibody, and the anode probe is a UiO66-NH2@luminol-labeled OTA antibody; glucose is added to the detection solution, and hydrogen peroxide is generated in situ through immobilized glucose oxidase, serving as the single co-reactant for both the cathode and anode. During detection, this invention generates H2O2 in situ through enzymatic generation, simultaneously exciting ECL signals at both the cathode and anode; it solves problems such as poor stability of the H2O2 co-reactant, inconvenient concentration control, multiple operation steps, and insufficient continuous supply; and it enables simultaneous quantitative detection of AFB1 and OTA with a single sample addition, without the need for external H2O2.

This invention belongs to the field of microbial electrochemistry and single-cell analysis technology, and specifically relates to a femtocoulomb-level single-bacterial surface charge imaging method and its application. This technology is based on electrochemiluminescence microscopy, utilizing the spontaneous enrichment of cationic ECL luminescent molecules by the electrostatic field of the negative charge on the bacterial surface, thereby non-destructively enhancing its ECL signal. By establishing a quantitative relationship between the ECL intensity enhancement ratio and the number of adsorbed molecules, and combining this with COMSOL simulation to determine the thickness of the luminescent layer, it achieves for the first time high-throughput, high spatiotemporal resolution dynamic quantitative measurement of femtocoulomb-level charges on the surface of a single living bacterium. The invented technology has high sensitivity, high resolution, and good biocompatibility, and can be used to reveal bacterial metabolic-charge regulation mechanisms, analyze synergistic electron transport pathways, and screen highly electroactive bacterial subpopulations in situ, providing a key tool for microbial electrochemical research.

This invention develops a pH-responsive controlled-release electrochemiluminescence sensor based on glucose oxidation and its application in the detection of CYFRA 21-1, belonging to the field of electrochemiluminescence sensor construction technology. The sensor constructed in this invention uses BSA / luminol-Ab2 / SiO2-PEI@Au NPs as the secondary antibody label and CeO2-Au as the substrate material. It utilizes the oxidation of glucose to gluconic acid under voltage, thereby lowering the pH. The sensor constructed in this invention has a wide detection range, high sensitivity, and low detection limit, providing a feasible solution for the detection of CYFRA 21-1.

To provide a small, easy-to-operate electrochemiluminescence sensor. [Solution] An electrochemiluminescence sensor for detecting a test substance in a sample using nanoparticles with an electrochemiluminescence immunoassay method utilizing a plasmon-enhanced field, comprising: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; the nanoparticles comprising: metal nanoparticles; a polymer film covering the surface of the metal nanoparticles; a second specific binding substance bound to at least one of the polymer film and the metal nanoparticles and specifically binding to the test substance; and an electrochemiluminescent substance bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant, wherein the electrochemiluminescence sensor comprises: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; and an electrochemiluminescent substance that is bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant.

This invention discloses a fully automated electrochemiluminescence (ECL) analysis system based on a local area network (LAN), relating to ECL detection technology. The system includes: an ECL instrument, used to receive control commands from a mobile terminal to start or stop the ECL detection process, and upon receiving a start command, to acquire and output signal data under voltage excitation conditions; a mobile terminal, which establishes a communication connection with the ECL instrument in a LAN or offline LAN environment, used to receive signal data, generate a local analysis file that can be saved on the mobile terminal based on the signal data, and perform a two-stage analysis method on the generated local analysis file, outputting the brightest frame target image and its corresponding ECL intensity value; and triggering a safety stop command to the ECL instrument when the mobile terminal malfunctions. This invention also discloses a detection method. This invention balances mobile terminal analysis efficiency and safety stop capability, greatly improving the analysis efficiency of the mobile terminal.

This work proposes a novel enzyme-free electrochemiluminescence sensing platform based on novel DNA-quantum dot nanostructures and hybridization chain reaction (HCR) amplification, and applies it to trace Cd. 2+ The detection; first, Cd 2+ Aptamer-triggered HCR amplification introduces a large amount of biotin-labeled DNA into the electrode, whereby biotin specifically captures a large amount of avidin SA-CdS quantum dot complexes, exhibiting a high ECL signal; target Cd 2+ Upon binding to its aptamer, the quantum dot-DNA structure detaches from the electrode, resulting in a significant decrease in the ECL signal, thus enabling the control of Cd. 2+ This sensor exhibits ultrasensitive detection with a dynamic response range from 10 fM to 10 nM and a detection limit of 2.6 fM. It demonstrates excellent selectivity, speed, high sensitivity, and practicality for detecting real water samples, providing a novel and competitive strategy for detecting heavy metal ions in real-world samples.

The application belongs to the technical field of electroluminescence, and particularly relates to a preparation method of a high-entropy oxide material and application of the high-entropy oxide material in electrochemiluminescence detection of levofloxacin. The high-entropy oxide material is a kind of five-element high-entropy oxide HEO-800 material with simple raw materials, convenient synthesis, excellent electrochemiluminescence performance, which is synthesized by taking metal nitrate of Fe, Co, Cu, Zn and Ni as raw materials, and taking Na2CO3 and NaOH as raw materials. The HEO-800 material has excellent conductivity and chemical stability, and a multi-electron reaction potential accelerates the conversion of dissolved oxygen into reactive oxygen species (ROS), enhances the electroluminescence intensity, and based on the quenching effect of LVX, a method for detecting LVX by using a luminol / HEO-800 system is established. By fitting the change of ECL intensity with the concentration of LVX, a linear calibration curve is obtained, the correlation coefficient is 0.993, and the detection limit is 1.02*10 ‑5 μM (S / N=3). The material has good sensitivity, stability and reproducibility for electrochemiluminescence detection of LVX, and provides a new scheme for designing a new electrocatalyst for testing LVX.

This invention relates to a near-infrared low-potential electrochemiluminescence dual-ligand stabilized gold nanoclusters, their preparation method, and applications. The method uses chloroauric acid as the gold source and thiomalic acid and sodium citrate as reducing and stabilizing agents, respectively, in a two-step synthesis. First, Au is reduced online using thiomalic acid. 3+ Water-soluble gold nanoclusters were prepared using a novel method. These nanoclusters were then incubated with sodium citrate, and based on the ligand exchange principle, gold nanoclusters co-coated with thiomalic acid and sodium citrate were synthesized. The raw materials required for this invention are readily available and all possess good water solubility. The synthesis apparatus is simple, the conditions are mild, and the operation is safe. The resulting dual-ligand-coated gold nanoclusters exhibit good stability and produce low-potential near-infrared electrochemiluminescence in the long-wavelength region in an aqueous system. This overcomes the limitation of most current electrochemiluminescent materials requiring hydrazine hydrate or carbazide as a co-reactant. Using a non-toxic tert-butylamine-borane co-reactant, low-potential electrochemiluminescence radiation of 0.57V in the near-infrared region was generated.

The present invention provides nanoparticles that can increase the intensity of electrochemiluminescence. [Solution] A nanoparticle body comprising metal nanoparticles, a polymer film coating the surface of the metal nanoparticles, a specific binding substance bonded to at least one of the metal nanoparticles and the polymer film and specifically binding to the test substance in the sample, and an electrochemiluminescent substance bonded to at least one of the polymer film and the specific binding substance.

This invention, for the first time, utilizes a novel self-luminescent copper-based metal-organic framework (Cu-MOF) to develop a potentiometric ECL biosensor for the simultaneous detection of two BRCA genes. The self-enhanced electrochemiluminescence Cu-MOF serves as the positive-potential ECL signaling material for the a-channel, with Au@AgNPs acting as a quencher; CdTe / CdS QDs serve as the negative-potential ECL luminescent material for the b-channel. Targeted cyclic amplification of DNA is employed. The Au@Ag NPs quencher is linked to the a-channel to quench the Cu-MOF ECL signal, while the CdTe / CdS QDs are linked to the b-channel, achieving zero-background ECL signal enhancement and enabling the simultaneous detection of BRCA1 and BRCA2. This invention overcomes the limitation of traditional potentiometric ECL biosensors requiring multiple co-reactants, providing a new approach to constructing ECL biosensors and demonstrating significant application potential in environmental monitoring, food safety, and disease analysis.

The present disclosure relates to novel multifunctionalized silicon nanoparticles, to processes for their preparation and to compositions comprising the novel multifunctionalized silicon nanoparticles. The disclosure also relates to the use of the novel multifunctionalized silicon nanoparticles in electrochemiluminescence based detection methods and in the in vitro detection of an analyte. In particular, the disclosure relates to methods for measuring an analyte by in vitro methods employing the novel multifunctionalized silicon nanoparticles.

This invention discloses a method for screening catalysts for the oxygen evolution reaction based on electrochemiluminescence quenching. The catalyst to be tested is loaded onto the working electrode, and then Ru(bpy)3 is added. 2+ In the electrolyte of / Hepes, applying an anodic potential to excite ECL accelerates the OER to generate O2, and O2 quenches the ECL signal. The signal intensity is negatively correlated with the catalytic activity. This method can be used for parallel detection on a single-electrode imaging array, achieving high throughput, visualization, and quantitative screening. It is applicable to various OER catalysts such as polyoxometalates and oxides, and has the advantages of being fast, low-cost, and easy to operate.

This invention discloses the construction and application of a biosensor based on autocatalytic induced emission amplification and a bifunctional peptide, belonging to the fields of electrochemiluminescence, sensing analysis, and nanomaterials technology. In this invention, by integrating recognition (HWRGWV), charge neutralization (DE), antifouling (DKDKDKDKDK), and anchoring (C) fragments, a bifunctional peptide with antibody-targeted immobilization and interfacial anti-adsorption properties was designed, improving the target capture efficiency and antifouling capability at the interface. Simultaneously, AuAgTb-MOF with a synergistic amplification effect was developed as the luminescent agent. Specifically, 3,5-dicarboxyphenylboronic acid is used to enhance the Tb... 3+ Antenna effect and Ag NPs on S2O8 2‑ The catalysis significantly promoted the luminescence of AuAgTb-MOF. The intramolecular self-enhanced design of AuAgTb-MOF not only reduced energy transfer loss but also simplified the construction of the biosensor. Based on this, the constructed biosensor exhibited a fluorescence intensity of 3.38 × 10⁻⁶. ‑5 The low detection limit of U / mL enables sensitive and accurate detection of the biomarker carbohydrate antigen 242, providing a positive reference for the clinical diagnosis of pancreatic cancer.

This invention belongs to the field of electroluminescence technology, specifically relating to a method for preparing a high-entropy MOF material and its application in the electrochemiluminescence detection of rifampicin. The high-entropy MOF material is a pentagonal high-entropy MOF material (HE-ATA) synthesized from metal nitrates of Fe, Co, Cu, Cr, and Zn and ATA as raw materials. This invention yields a HE-ATA catalyst with simple raw materials, convenient synthesis, and excellent electrochemiluminescence performance. It exhibits chemical stability and a multi-electron reaction potential that accelerates the conversion of dissolved oxygen to reactive oxygen species (ROS), enhancing the electroluminescence intensity. Based on the quenching effect of RIF, a method for detecting RIF using a luminol / HE-ATA system was established. By fitting the change in ECL intensity with RIF concentration, a linear calibration curve with a correlation coefficient of 0.996 and a detection limit of 3.3 × 10⁻⁶ was obtained. ‑6 µM (S / N=3). This material exhibits good sensitivity, stability, and reproducibility in the electrochemiluminescence detection of RIFs, providing a new approach for designing novel electrocatalysts for RIF detection.

This invention belongs to the field of biodetection technology and provides a lateral flow immunochromatographic membrane and biosensor based on electrochemiluminescence. The lateral flow immunochromatographic membrane includes a membrane body, a wax layer fixedly coated on the surface of the membrane body, control lines, and conductive electrodes, as well as detection lines fixedly coated on the surface of the conductive electrodes. The wax layer has lateral flow channels arranged along the length of the membrane body. The conductive electrodes span the lateral flow channels, with their two ends fixedly coated on the surface of the wax layer. The conductive electrodes and control lines are spaced apart along the length of the membrane body. The lateral flow immunochromatographic membrane and biosensor provided by this invention combine qualitative screening with visualized results and high-sensitivity quantitative analysis, offering simple operation, short detection time, and improved detection efficiency and reliability.

This invention relates to the field of biosensor technology, and in particular to an electrochemiluminescence sensor, its fabrication method, and its applications. The sensor comprises a three-electrode system, wherein the working electrode includes a substrate electrode, an amino-functionalized vertically ordered mesoporous silica film formed on the surface of the substrate electrode, and nitrogen-doped graphene quantum dots confined within the amino-functionalized vertically ordered mesoporous silica film; the electrolyte used in the sensor is a phosphate buffer containing luminol. This invention achieves highly sensitive and selective detection of superoxide dismutase (SOD) by constructing an electrode modified with nitrogen-doped graphene quantum dots confined within an amino-functionalized vertically ordered mesoporous silica film, utilizing its enhanced luminol-dissolved oxygen electrochemiluminescence signal and the specific quenching effect of SOD on superoxide anions.

The invention discloses a titanium-doped zirconium MOF molecularly imprinted electrochemical luminescence sensor for high-sensitivity detection of HER2, and a preparation and detection method thereof, and belongs to the technical field of detection. A titanium-doped zirconium-based metal organic framework (Ti-Zr-TPE MOF) is dispensed on the surface of a working electrode to serve as a luminescent material, Ti-Zr-TPE realizes luminous intensity multiplication in an aggregation state compared with Zr-TPE by further enhancing an intra-molecular motion limited (RIM) mechanism, and a luminous body preparation process, co-reactant screening and detection parameters (such as potential scanning rate and pH value) are systematically optimized, so that the luminous intensity of Ti-Zr-TPE is improved. A set of stable Ti (at) Zr-TPE luminescent system is formed. Meanwhile, dopamine (DA) is used as a functional monomer, and HER2 is subjected to molecular imprinting through a molecular imprinting technology, so that the electrochemical luminescence sensor of a quenching system is constructed. The luminescent material based on the novel tetraphenyl ethylene (TPE) derivative has the advantages of high luminescent intensity, low background signal, high stability and specificity, simplicity and convenience in operation and the like, and has obvious advantages compared with a previously developed sensor for detecting HER2. According to the invention, quantitative detection of HER2 can be realized, and an innovative solution is provided for clinical early warning.

This invention belongs to the field of electrochemiluminescence and provides an electrochemiluminescence aptamer sensor for the detection of isobutyryl fentanyl (IBF), its preparation, and its application. An apt@Ni-MOFs / cDNA / Ag-MOGs / SiO2 electrode with electroluminescence and specific recognition properties is modified onto the surface of a glassy carbon electrode (GCE) to form the modified electrode apt@Ni-MOFs / cDNA / Ag-MOGs / SiO2 / GCE. This modified electrode is used as the working electrode, an Ag / AgCl electrode as the reference electrode, and a platinum electrode as the counter electrode. IBF was detected by electrochemiluminescence in a PBS buffer solution containing 0.15 mol / L K2S2O8 at pH 7.4, and the corresponding regression equation was obtained; ΔECL = 2.4 × 10⁻⁶. 4 + 1.6×10 3 ×log C (g / L), correlation coefficient R 2 =0.999, detection limit is 3.3 × 10 –15 g / L. This invention exhibits good selectivity, high sensitivity, and a wide linear range.

This utility model belongs to the technical field of electrochemical detection equipment, and provides a portable electrochemiluminescence detection device. It includes a dark box housing with a main unit compartment and a detection compartment, a photon counting unit fixedly installed in the main unit compartment, and an electrochemical pressure application unit fixedly installed in the detection compartment. A horizontal partition inside the dark box housing divides its internal space into a top main unit compartment and a bottom detection compartment. The horizontal partition has a detection through-hole connecting the main unit compartment and the detection compartment. The photon counting unit performs electrochemiluminescence detection on the sensor to be tested connected to the electrochemical pressure application unit through the detection through-hole. The portable electrochemiluminescence detection device provided by this utility model has a simple structure and is easy to use, reducing the operational difficulty and complexity of electrochemiluminescence detection, improving the efficiency of electrochemiluminescence detection, and is easy to carry and transport for on-site testing. It is highly practical and has a wide range of applications.

The application discloses a kind of silicon heteroanthracene derivatives with green aggregation-induced emission characteristics and synthesis method and application, belong to luminescent material technical field.The structural formula of the derivative is with silicon heteroanthracene as core, and 9,9-dimethylfluorene group is connected on both sides.The introduction of 9,9-dimethylfluorene forms twisted non-planar structure, so that the luminescence of molecule is significantly enhanced in aggregated state, AIE characteristics are obtained, while retaining the inherent σ* of silicon heteroanthracene-pi* conjugation effect and low LUMO energy level advantage.The synthesis method is based on suzuki coupling reaction, with 2,7-dichloro-9,9-dimethyl-9H-9-silicon heteroanthracene and 9,9-dimethylfluorene-2-boronic acid pinacol ester as raw material, under the action of catalyst, phosphine ligand and base in ethanol / water mixed solvent, mild condition, simple operation.The derivative can be used as efficient green light emitting material in electrochemiluminescence field, especially as electrochemiluminescence luminophor for detection of quercetin, with high detection sensitivity, good selectivity and strong anti-interference ability.

The present application relates to a kind of based on electrochemiluminescence immunoassay semeglu-tide content detection method, the kit for detecting semeglu-tide and monoclonal antibody pair, it solves the technical problem that the prior art through LC-MS / MS determination semeglu-tide content exists and is complex, detection flux is low, impurity residue, sample loss amount is big and traditional ELISA detection sensitivity is low, it is by the way that one monoclonal antibody reagent AS-MAB01 is coated on electrochemiluminescence microplate, specifically recognizes and combines semeglu-tide in matrix, then another monoclonal antibody AS-MAB02 labeled with ruthenium is added to form sandwich complex, finally, high sensitivity semeglu-tide quantitative detection purpose is achieved by electrochemiluminescence principle.The present application can be used for the quantitative detection of semeglu-tide in mouse, monkey and human-derived serum samples.

The application discloses a biosensor and a method for detecting NF-kappa B p50, comprising: an electrode, wherein a DNA tetrahedron structure is combined on the surface of the electrode; the DNA tetrahedron structure is used for being combined with a detection probe DNA; the detection probe DNA comprises a detection probe DNA nucleic acid sequence and an electrochemiluminescence group connected with the detection probe DNA nucleic acid sequence. The biosensor can realize reversible dissociation of the detection probe DNA under alkaline conditions, thereby supporting at least 10 repeated detection cycles without affecting detection sensitivity and specificity. The detection method adopts a differential pulse voltammetry method, combines with a side branch cutting signal amplification strategy of a CRISPR-Cas12a system, and realizes high-sensitivity detection of the NF-kappa B p50. The method is suitable for complex biological samples, has high sensitivity, high specificity and good repeated use performance, and the minimum detection limit can reach 100 femtomolar level, and the linear detection range covers 100 aM to 80000 aM.

This invention belongs to the field of biosensors and electrochemiluminescence (ECL) detection technology, and discloses an electrochemiluminescence sensor based on a UiO-67 confined single-site iridium complex, its construction method, and applications. The system uses zirconium-based metal-organic framework (MOF) material UiO-67 as a carrier, and iridium(III) bridging compounds are bonded through coordination to construct an in-situ Ir@UiO-67 nano-signal probe with high ECL activity. This probe is labeled with P-tau 181 secondary antibody to obtain an Ab2-Ir@UiO-67 complex. Simultaneously, a boron-doped diamond electrode modified with a gold film is used as the substrate electrode, and P-tau 181 primary antibody is immobilized to construct a specific recognition interface. A highly sensitive electrochemiluminescence sensing platform is then assembled via a sandwich immunoassay. This invention improves upon the bottleneck problems of poor water solubility and easy aggregation and quenching of traditional iridium complexes through MOF coordination anchoring and pore spatial confinement effects, achieving ultrasensitive quantitative detection of P-tau 181. It exhibits excellent specificity, reproducibility and stability in plasma samples.

The application discloses an electrochemiluminescence aptamer sensor for detecting methcathinone (Met) and a preparation method thereof. A cDNA / Au NPs-Apt&C4 DNA / Cu2O / ZnO / Z-MCOF / GCE modified electrode is used as a working electrode, an Ag / AgCl electrode is used as a reference electrode, and a platinum electrode is used as an auxiliary electrode to form a traditional three-electrode system for quantitatively detecting Met. The sensor shows excellent analytical performance for Met based on a "off-on-off" strategy: a good linear relationship is shown in a concentration range of 1.0*10 ‑15 to 1.0*10 ‑7 g / L, and a detection limit is as low as 9.11*10 ‑16 g / L. The application has the advantages of high sensitivity, good selectivity, strong anti-interference capability and the like, and provides a novel technology for rapidly detecting synthetic drugs in various complex substrates such as alcoholic beverages.

The application discloses a three-dimensional bipolar ECL lateral flow immunochromatographic test strip and application thereof in immunodetection, and relates to the technical field of immunodetection. The three-dimensional bipolar ECL lateral flow immunochromatographic test strip comprises a three-dimensional bipolar electrode sheet, a lateral flow immunochromatographic test strip and a connecting pad; the three-dimensional bipolar electrode sheet is arranged below, the lateral flow immunochromatographic test strip and the connecting pad are arranged above; the three-dimensional bipolar electrode sheet is designed in a layered mode and comprises an electrode sheet layer one and an electrode sheet layer two; the electrode sheet layer two is arranged below, and the electrode sheet layer one is arranged above; the electrode sheet layer two comprises a positive driving electrode; the positive driving electrode comprises a positive driving electrode head end, a positive driving electrode tail end and connecting wires thereof. The three-dimensional bipolar electrode sheet is designed for the first time, the electrode sheet layer one and the electrode sheet layer two are combined through back glue with adhesion, the shortcomings of a traditional bipolar ECL electrode sheet are broken, and the three-dimensional bipolar ECL lateral flow immunochromatographic test strip is more suitable for the demand of on-site rapid detection.

This invention discloses an electrochemiluminescence microfluidic chip for the detection of multiple tumor markers, relating to the field of biodetection technology. It includes a substrate and a microfluidic channel system, an electrochemiluminescence detection unit, and a signal acquisition interface integrated on the substrate. The microfluidic channel system includes a sample inlet, a shunt structure, multiple parallel and equally spaced detection channels, and a waste liquid pool connected in sequence. The shunt structure includes a main channel and multiple branch channels integrally formed with the main channel. In this invention, the microfluidic channel system, the electrochemiluminescence detection unit, and the signal acquisition interface are integrated on the same substrate, achieving an integrated design for sample transmission, reaction, detection, and signal export. Compared to traditional distributed detection technologies, this significantly simplifies the detection operation steps, reduces the professional skills required of operators, and shortens the detection cycle, meeting the needs of rapid clinical diagnosis.

This invention discloses an optical CCD vision inspection system and method for lithium battery components. The system includes a multi-physics sensing module, an interference quantification module, a CCD image acquisition module, a hardware / software co-control module, a signal correction module, and a defect confidence assessment module. The multi-physics sensing module synchronously acquires data such as vibration displacement and electrochemiluminescence intensity and transmits it to the interference quantification module. After calculating the interference coefficient, the hardware / software co-control module adjusts the CCD parameters. The signal correction module adaptively corrects the original grayscale signal. The defect confidence assessment module calculates the confidence level based on multi-dimensional features and outputs the result. The method executes the corresponding steps, solving the problem of defect misjudgment caused by prior art's failure to consider multi-physics interference, and achieving accurate detection of minute defects in lithium battery components.