412 results about "Electrochemiluminescence" patented technology

The invention discloses a high-sensitivity cardiac troponin-T electrochemiluminescence detection kit. The kit comprises a magnetic microsphere reagent coated by a high-sensitivity cardiac troponin-T capture antibody and a high-sensitivity cardiac troponin-T detection antibody reagent labeled by an electrically neutral ruthenium compound, the structural general formula of the electrically neutral ruthenium compound is # imgabs0 # n = 0-9; the magnetic microsphere reagent coated with the high-sensitivity cardiac troponin-T capture antibody comprises a magnetic microsphere coated with the high-sensitivity cardiac troponin-T capture antibody and a diluent; the electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody reagent comprises an electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody and a diluent; the diluent is prepared from piperazine-1, 4-diethyl sulfonic acid or 3-(N-morpholinyl)-2-hydroxy propanesulfonic acid. When the high-sensitivity cardiac troponin-T electrochemiluminescence detection kit is used for detection, a reaction system does not contain streptavidin coated magnetic beads or biotinylated antibodies, so that interference of biotin in a sample is avoided.

The invention discloses an immunosensor for detecting cytokine interferon gamma through electrochemiluminescence as well as a preparation method and application of the immunosensor. The immunosensor comprises a three-electrode system consisting of an immunoelectrode, a reference electrode and a counter electrode, the immunoelectrode is a VMSF modified electrode confined to cerium dioxide nano enzyme and platinum nano particles, and is obtained by covalently immobilizing an IFN-gamma antibody and closing a non-specific site; when the immunosensor is used for detection, a solution containing luminol is used as an electrolyte. The CeO2 and the PtNPs which are confined in the nano channel show a remarkable synergistic catalysis effect. Compared with direct modification on a plate electrode, CeO2-PtNPs has higher catalytic activity and stability. The sensor provided by the invention is used for INF-gamma detection, has the advantages of high sensitivity, low detection limit and wide detection range, does not need complex pretreatment and separation processes during detection, reduces the detection cost, is simple to operate, and is suitable for large-scale application.

The invention belongs to the technical field of immunoassay and biosensing detection, and particularly relates to a preparation method and application of an antibiotic electrochemical luminescence sensor based on a double-enhancement strategy. According to the invention, a bifunctional metal organic gel is used as a substrate, a graphite-phase carbon nitride quantum dot is used as a luminous body, and a self-assembly split aptamer walker is combined to construct the electrochemical luminescence sensor for sensitive detection of antibiotics. The sensor has the advantages of extremely high specificity and sensitivity, wide detection range, low detection limit and strong anti-interference performance, and has important scientific significance and application value in detection of antibiotic residues in the environment.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention relates to the field of electrochemiluminescence imaging analysis, and particularly discloses a single-particle electrochemiluminescence imaging method by adjusting the thickness of a molecular self-assembly layer, which is used for solving the problems of serious non-specific luminescence of an electrode substrate, incapability of finely regulating an interface structure, poor stability and the like in single-particle electrochemiluminescence imaging. The method comprises the following steps: preparing an insulating molecule self-assembly layer with gradient thickness, optimizing a fixing process of gold nanoparticles, determining the position of a single particle, carrying out ECL imaging acquisition on the single particle, and optimizing the thickness of the molecule self-assembly layer. According to the method, the thickness of a molecular self-assembly layer is precisely regulated and controlled, a nano-particle fixing and ECL system is optimized, and a single-particle ECL imaging device is built, so that the signal-to-noise ratio of single-particle electrochemical imaging can be increased, and background interference caused by non-specific luminescence of an electrode substrate is effectively inhibited.

The application discloses an electrochemiluminescence immunosensor for detecting interleukin-1 beta and a preparation method and application thereof, and comprises a three-electrode system of a working electrode, a reference electrode and a counter electrode; the working electrode is a modified electrode of CoAl layered double hydroxide, is loaded with platinum nanoparticles after being etched by alkali, and is obtained by fixing interleukin-1 beta antibodies and blocking non-specific sites; and a solution containing luminol is used as an electrolyte during detection of the immunosensor. The working electrode of the immunosensor takes oxygen-rich vacancy CoAl layered double hydroxide as a substrate, combines with platinum nanoparticles to realize EMSI effect, significantly enhances the electrochemiluminescence signal of a luminol-dissolved oxygen system, realizes quantitative detection of IL-1 beta by combining with specific antigen-antibody recognition, and has the characteristics of strong anti-interference ability, fast detection speed and good stability.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The invention relates to the technical field of electrochemical luminescence detection, in particular to an electrochemical luminescence aptamer sensor based on a CoOOH-cDNA / Apt / AgNWs / Tb (at) A-COF / GCE working electrode. The iBF in a sample to be detected is detected by utilizing LC-MS / MS, GC-MS, UHPLC and HPLC-MS, so that the cost is high, the speed is low, and the sensitivity is relatively low. Aiming at the technical problem, the invention provides the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE working electrode, the working electrode takes Tb-coated A-COF as a donor and CoOOH NSs as a receptor, the donor and the receptor are combined and modified on a glassy carbon electrode (GCE) through an aptamer and cDNA, then the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE is taken as the working electrode, and iBF in a sample to be detected is quantitatively detected. According to the invention, through a signal "on-off-on" (on-off-on) mode, the consistency of detection results is significantly improved, the generation of false positive or false negative signals is reduced, and the detection accuracy is improved.

The present invention is directed to assay methods and compounds and kits used in such assays.

The invention discloses a biosensor and a method for detecting NF-kB p50, the biosensor comprises: an electrode, the surface of which is bound with a DNA tetrahedral structure; the DNA tetrahedral structure is used for being combined with a detection probe DNA; the detection probe DNA comprises a detection probe DNA nucleic acid sequence and an electrochemical luminescence group connected with the detection probe DNA nucleic acid sequence. The biosensor can realize reversible dissociation of detection probe DNA under an alkaline condition, so that at least 10 times of repeated detection cycles are supported, and the detection sensitivity and specificity are not influenced. According to the detection method, a differential pulse voltammetry is adopted, a collateral cleavage signal amplification strategy of a CRISPR-Cas12a system is combined, high-sensitivity detection of NF-kappa B p50 is achieved, the method is suitable for complex biological samples and has high sensitivity, high specificity and good reuse performance, the lowest detection limit can reach the 100 fly mole level, and the linear detection range covers 100 aM to 80000 aM.

The invention discloses a full-automatic electrochemical luminescence detector based on a photodiode, and relates to an electrochemical luminescence detector, the full-automatic electrochemical luminescence detector comprises a shell, the shell is internally provided with an optical signal acquisition module, a small packaging circuit board, an ECL chip, a man-machine interaction module and a chip inlet and outlet device; a light current detection unit, an electrochemical reaction excitation unit and a microcontroller are arranged in the small packaging circuit board; wherein a test strip sample pad and a reaction area are arranged in the ECL chip. The photodiode used in the invention can make a quick response to the change of light intensity, which is far faster than the frame capturing speed of the camera, and the demand and complexity of subsequent data processing are reduced, so that the system is easier to realize automation. Compared with a camera, the photodiode is lower in hardware cost, does not need to use complex image processing software, and is suitable for household use. The detector is compact in structure, small and exquisite in appearance and convenient to use in a handheld mode and carry about.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

The invention relates to the technical field of electrochemical luminescence detection application, and discloses a single molecule detection method based on AI and electrochemical luminescence, and the method comprises the following steps: collecting electrochemical luminescence signal data of a sample to generate a data set, and receiving real-time signals to construct a matrix; processing the signal data matrix, extracting features in combination with the data set, predicting single molecule distribution according to the signal intensity gradient and the detection path information, outputting space-time distribution features through an AI model, and updating the data set; detection parameters such as sensor sensitivity, light source intensity and sampling frequency are dynamically adjusted according to distribution characteristics. According to the method, through combination of AI and electrochemical luminescence, determination of single molecule detection characteristics and dynamic adjustment of detection parameters are realized, and the accuracy and efficiency of single molecule detection are improved.

The invention belongs to the technical field of electrochemical luminescence detection equipment, and particularly relates to a microneedle patch for wearable electrochemical luminescence sensing equipment and application of the microneedle patch. The microneedle patch comprises a microneedle base and a microneedle array, the microneedle base is divided into a left area, a middle area and a right area through two parallel grooves; the middle area is conductive and light-transmitting, and the left and right areas are conductive and light-proof; the microneedle arrays are arranged in the three areas and are all subjected to conductive treatment; the three areas are separated by grooves and are insulated from one another and are not conductive; and the surface in each area has conductive performance, so that electric signals can be conducted. The light-emitting sensing equipment takes the microneedle patch as a real-time acquisition tool of a tissue fluid sample, detects the concentration of cTnI in the tissue fluid on line, realizes real-time monitoring of a marker cTnI of myocardial injury and acute myocardial infarction in vivo, has the characteristics of high detection sensitivity, portability, simplicity in operation, low cost and the like, and realizes online in-situ monitoring of wearable electrochemical luminescence of a patient.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention belongs to the technical field of biochemical detection, and particularly relates to a bionic microsphere, a non-diagnostic-purpose extracellular vesicle detection method and an extracellular vesicle detection kit, the bionic microsphere is prepared by modifying a homologous cell membrane CM on the surface of a silica microsphere MS; the bionic microsphere (CMS) is used for capturing specific types of extracellular vesicles (EVs) in a to-be-detected sample and an optical / electrochemical detection method, can specifically capture homologous EVs, and is combined with fluorescence, ultraviolet visible absorption, differential pulse voltammetry and electrochemical luminescence for detection.

The invention relates to a lanthanide oxide electrochemiluminescence aptamer sensor with dual signal amplification for detecting acetamiprid as well as a preparation method and application of the lanthanide oxide electrochemiluminescence aptamer sensor. According to the sensor, terbium-doped europium oxide (Eu2O3: Tb < 3 + >) modified by gold nanoparticles (Au NPs) is used as a luminous body, and the electrochemical luminescence performance can be remarkably improved through multiple mechanisms: on one hand, the Au NPs can effectively promote generation of more sulfate free radicals; on the other hand, the surface defect passivation effect and the efficient synergistic effect of energy transfer jointly achieve effective amplification of signals. In addition, the wool ball-shaped Ag NPs is used as a plasma resonance signal amplification source, so that the ECL intensity of the sensor is further enhanced. The prepared electrochemical luminescence sensor with the dual signal amplification effect is used for detecting acetamiprid (ACE), has a wide linear range and a low detection limit, and has a wide application prospect in detection of acetamiprid residues in a water environment.

The invention belongs to the technical field of electrochemical luminescence analysis and detection, and relates to an electrochemical luminescence sensor of a defective iron metal organic framework enhanced pyrenyl self-assembled hydrogen bond organic framework, and a preparation method and application thereof. Comprising the following steps: dispensing a PSA-HOF / Pca-MIL-88B (Fe) dispersion liquid on a GCE electrode, drying, dropwise adding a perfluorooctanoic acid aptamer, reacting at room temperature to finally obtain an aptamer modified PSA-HOF / Pca-MIL-88B (Fe) electrode as a working electrode, Ag / AgCl as a reference electrode and a Pt electrode as a counter electrode, and introducing the aptamer modified PSA-HOF / Pca-MIL-88B (Fe) electrode, Ag / AgCl as a reference electrode and a Pt electrode as a counter electrode into a PBS phosphate buffer solution containing potassium persulfate for detecting perfluorooctanoic acid. The prepared PSA-HOF / Pca-MIL-88B (Fe) system electrochemical luminescence sensor has the characteristics of simplicity, convenience and flexibility in operation, wide detection range, low detection limit, low detection cost and the like.

The invention provides a preparation method and application of an electrochemical luminescence aptamer sensor for detecting perfluorooctanoic acid (PFOA) in a water environment by adopting an apt / CHIT / Zn TCPP (at) MOF / GCE modified electrode based on an electrochemical luminescence method. The preparation method comprises the following steps: electrically depositing a zinc tetraphenylporphyrin functionalized metal organic framework (Zn TCPP-coated MOF / GCE) on a glassy carbon electrode (GCE), and further modifying an aptamer (apt) of chitosan (CHIT) and PFOA to prepare an apt / CHIT / Zn TCPP-coated MOF / GCE modified electrode. The modified electrode apt / CHIT / Zn TCPP (at) MOF / GCE prepared on the basis of an electrodeposition technology is low in detection limit and good in reproducibility, pretreatment of concentration and enrichment on a water environment sample is not needed due to high sensitivity, and analysis operation on PFOA is simple and easy to implement.

The invention discloses a confinement composite nano-enzyme enhanced electrochemiluminescence immunosensor for detecting tumor necrosis factors and a construction method and application of the immunosensor. The immunosensor is a three-electrode system, and liquid to be detected contains luminol; wherein the working electrode is obtained by covalently immobilizing a tumor necrosis factor specific antibody after an SNF epoxy modified electrode is subjected to confinement of Ni (OH) 2-CeO2 composite nano-enzyme and then closing a non-specific site. The Ni (OH) 2-CeO2 composite nano-enzyme is confined in a nano-channel, the Ni (OH) 2-CeO2 composite nano-enzyme and the nano-channel can synergistically accelerate the process of an oxygen reduction reaction, the modification stability of the nano-enzyme is effectively improved, and a formed immune complex causes increase of interface resistance and steric hindrance of a sensing electrode to cause signal reduction; therefore, high-sensitivity detection of the tumor necrosis factor is realized, and the constructed sensor has the advantages of low detection limit, wide detection range and the like, and is very suitable for being widely applied to the fields of auxiliary clinical diagnosis, large-scale screening, health monitoring and the like.

The invention is suitable for the field of electrochemical luminescence detection equipment, and provides a dispersion device suitable for electrochemical luminescence detection, which comprises a monochromator body, the monochromator body is composed of a monochromator housing, a reflector assembly, a grating assembly, a collimating mirror assembly, a focus lens assembly, a slit, a flange snap ring and a flange; an M-shaped light path is arranged in the monochromator body, and a slit, a reflector assembly, a collimating mirror M1 in the collimating mirror assembly, a customized blazed grating G1 in the grating assembly and a focusing mirror M2 in the focusing mirror assembly are sequentially arranged in the light propagation direction. The customized blazed grating G1 is designed by adopting specific parameters including the scribed line density, the blazed wavelength, the notch groove depth and the groove width ratio, and primary light energy enhancement and secondary light intensity suppression can be realized; according to the invention, an optical filter is not needed, the diffraction efficiency of a monochromator is improved to 60-86%, the intensity ratio of luminol secondary light to primary light is less than or equal to 1.6%, the detection limit of ruthenium bipyridine is reduced from 100pmol / L to 50pmol / L, and the electrochemical luminescence detection device is suitable for various electrochemical luminescence detection scenes.

The invention belongs to the technical field of biosensors, and provides a tobacco mosaic virus (TMV) dual-mode biosensor as well as a preparation method and application thereof. The biosensor is composed of a dual-mode luminescent material Co / Ce-TCBPE (at) Ru (dcbpy) 32-COOH, a lateral flow chromatography LFIA flexible electrode, a TMV antibody Ab1, a TMV antibody Ab1 and a self-assembled micro electrochemical luminescence (ECL) sensor, and through qualitative and quantitative dual-signal output of vision and ECL detection, the TMV detection sensitivity and accuracy are remarkably improved. The detection range of the sensor is 0.1 pg / mL-100 ng / mL, the detection limit reaches 48.9 fg / mL, and the sensor has the advantages of simplicity and convenience in operation, quick response, high stability, dual signal guarantee and the like, is suitable for quick visual detection of plant diseases, and has a wide application prospect in agricultural detection.

The invention discloses an electrochemical microscopic imaging analysis method based on a fluorescence signal, which comprises the following steps of: constructing a fluorescence response electrochemical signal instrument by combining electrochemistry with a fluorescence microscope, and observing the redox potential of K3 [Fe (CN) 6] by taking a terpyridyl ruthenium (Ru (bpy) 32 +) / potassium ferricyanide (K3 [Fe (CN) 6]) fluorescence system as a research object. Influences of electrochemical behaviors on fluorescence signals are deeply explored, and the relationship between fluorescence signal changes and electric signal changes is revealed. According to the present invention, the combination of the electrochemical technology and the fluorescence signal is achieved, the disadvantage of the poor spatial-temporal resolution of the electrochemiluminescence is made up, the new dimension capable of being regulated and controlled by the electrical parameter is provided for the fluorescence imaging, and the Faraday current and the non-Faraday current can be completely reflected by the fluorescence signal. Therefore, the method has wide application value in the aspects of exploring the conductivity of the nano material and the like.

The invention relates to the technical field of organic detection, in particular to a nanoparticle modified quantum dot MOF material and a preparation method and application thereof. A zirconium-based double-ligand metal organic framework material is used as a carrier, a co-reaction accelerant Ti3C2 quantum dot is modified to enhance the electrochemical luminescence reaction efficiency, and copper-gold nanoparticles are loaded to amplify signals. The nanoparticle modified quantum dot MOF material provided by the invention can be prepared into an aptamer biosensor for accurately identifying malathion, and has high sensitivity and high selectivity. According to the invention, a nanoparticle modified quantum dot MOF material is coupled and combined with an aptamer, and an aptamer probe with high electrochemical luminescence activity and specific sensitivity to malathion is constructed. When the malathion acts on the aptamer probe, the malathion is combined with the aptamer in a high affinity manner, the aptamer probe is separated from the surface of the electrode, and meanwhile, an electrochemical luminescence signal is changed, so that the malathion residue in the agricultural product is rapidly detected.

The invention relates to the technical field of mycotoxin rapid detection, in particular to an electrochemical luminescence sensor for citrinin detection and a detection method of the citrinin. A sensing substrate of the sensor is prepared by loading platinum nanoclusters (Pt NCs) on a NiCo-LDH / MXene composite substrate through an in-situ growth strategy; the high conductivity of MXene and the LDH confinement effect are utilized to synergistically promote three-dimensional exposure of Pt active sites, the efficiency of generating active oxygen through electro-catalysis is remarkably enhanced, and a light-emitting signal is improved. And a CRISPR / Cas12a system is further integrated, so that a regulation mechanism of recognition-cutting-signal de-suppression is realized, and food matrix interference is effectively avoided. And a new technology is provided for monitoring the fungaltoxin by combining a dual enhancement mechanism of material catalytic optimization and enzymatic signal amplification.

The invention belongs to the field of functional nano materials and optical imaging, and particularly relates to a gold nanocluster and a preparation method and application thereof. The surface of the gold nanocluster contains a sulfydryl ligand A and a sulfydryl ligand B, the sulfydryl ligand A is ATT, and the sulfydryl ligand B is a sulfydryl ligand containing arginine; fluorescence quantum efficiency and electron transfer efficiency are synergistically enhanced on the basis of interaction of hydrogen bonds in the gold nanoclusters, the ultra-small luminous gold nanoclusters protected by double ligands are synthesized, the electrochemical luminescence quantum efficiency of the ultra-small luminous gold nanoclusters is up to 13.0% and is 4.2 times of that of a standard compound terpyridyl ruthenium in the field of electrochemical luminescence, and the ultra-small luminous gold nanoclusters are high in electrochemical luminescence quantum efficiency. An electrochemical luminescence transition area can be narrowed by 10 times compared with a terpyridyl ruthenium imaging system, and zero-thickness ECL luminescent layer cell high-resolution imaging can be realized; good application prospects are realized in the fields of fluorescence detection, electrochemical luminescence detection, fluorescence imaging, electrochemical luminescence imaging and the like.

The invention discloses an immunosensor for detecting Alzheimer's disease neurofilament light chain protein based on a two-dimensional metal organic framework and a preparation method of the immunosensor, and belongs to the technical field of biological detection sensors. An electrode modified with a RuMOFNSs-Ab2 conjugate and an Au-coated MoS2-Ab1 conjugate is used as a working electrode; the sandwich type electrochemical luminescence immunosensor is based on the two-dimensional RuMOFNSs / Au-coated MoS2, can be used for detecting the blood marker NfL of the Alzheimer's disease, provides a thought for the field of analysis and detection of the NfL, and also provides a reference for clinical medicine detection.

The invention discloses an electrochemical luminescence immunosensor for detecting interleukin-1 beta as well as a preparation method and application of the electrochemical luminescence immunosensor. The electrochemical luminescence immunosensor comprises a three-electrode system consisting of a working electrode, a reference electrode and a counter electrode, the working electrode is a modified electrode of CoAl layered double hydroxides, the modified electrode is subjected to alkali etching and then loaded with platinum nanoparticles, then an interleukin-1 beta antibody is fixed, and non-specific sites are closed, so that the working electrode is obtained; when the immunosensor is used for detection, a solution containing luminol is used as an electrolyte. The working electrode of the immunosensor takes oxygen vacancy-rich CoAl layered double hydroxide as a substrate, platinum nanoparticles are combined to realize an EMSI effect, an electrochemical luminescence signal of a luminol-dissolved oxygen system is remarkably enhanced, and antigen-antibody specific recognition is combined to realize quantitative detection of IL-1beta. The method has the characteristics of strong anti-interference capability, high detection speed and good stability.