243 results about "Electrochemiluminescence" patented technology

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention relates to the field of electrochemiluminescence imaging analysis, and particularly discloses a single-particle electrochemiluminescence imaging method by adjusting the thickness of a molecular self-assembly layer, which is used for solving the problems of serious non-specific luminescence of an electrode substrate, incapability of finely regulating an interface structure, poor stability and the like in single-particle electrochemiluminescence imaging. The method comprises the following steps: preparing an insulating molecule self-assembly layer with gradient thickness, optimizing a fixing process of gold nanoparticles, determining the position of a single particle, carrying out ECL imaging acquisition on the single particle, and optimizing the thickness of the molecule self-assembly layer. According to the method, the thickness of a molecular self-assembly layer is precisely regulated and controlled, a nano-particle fixing and ECL system is optimized, and a single-particle ECL imaging device is built, so that the signal-to-noise ratio of single-particle electrochemical imaging can be increased, and background interference caused by non-specific luminescence of an electrode substrate is effectively inhibited.

The application discloses an electrochemiluminescence immunosensor for detecting interleukin-1 beta and a preparation method and application thereof, and comprises a three-electrode system of a working electrode, a reference electrode and a counter electrode; the working electrode is a modified electrode of CoAl layered double hydroxide, is loaded with platinum nanoparticles after being etched by alkali, and is obtained by fixing interleukin-1 beta antibodies and blocking non-specific sites; and a solution containing luminol is used as an electrolyte during detection of the immunosensor. The working electrode of the immunosensor takes oxygen-rich vacancy CoAl layered double hydroxide as a substrate, combines with platinum nanoparticles to realize EMSI effect, significantly enhances the electrochemiluminescence signal of a luminol-dissolved oxygen system, realizes quantitative detection of IL-1 beta by combining with specific antigen-antibody recognition, and has the characteristics of strong anti-interference ability, fast detection speed and good stability.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The present invention is directed to assay methods and compounds and kits used in such assays.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

The invention relates to a lanthanide oxide electrochemiluminescence aptamer sensor with dual signal amplification for detecting acetamiprid as well as a preparation method and application of the lanthanide oxide electrochemiluminescence aptamer sensor. According to the sensor, terbium-doped europium oxide (Eu2O3: Tb < 3 + >) modified by gold nanoparticles (Au NPs) is used as a luminous body, and the electrochemical luminescence performance can be remarkably improved through multiple mechanisms: on one hand, the Au NPs can effectively promote generation of more sulfate free radicals; on the other hand, the surface defect passivation effect and the efficient synergistic effect of energy transfer jointly achieve effective amplification of signals. In addition, the wool ball-shaped Ag NPs is used as a plasma resonance signal amplification source, so that the ECL intensity of the sensor is further enhanced. The prepared electrochemical luminescence sensor with the dual signal amplification effect is used for detecting acetamiprid (ACE), has a wide linear range and a low detection limit, and has a wide application prospect in detection of acetamiprid residues in a water environment.

The invention is suitable for the field of electrochemical luminescence detection equipment, and provides a dispersion device suitable for electrochemical luminescence detection, which comprises a monochromator body, the monochromator body is composed of a monochromator housing, a reflector assembly, a grating assembly, a collimating mirror assembly, a focus lens assembly, a slit, a flange snap ring and a flange; an M-shaped light path is arranged in the monochromator body, and a slit, a reflector assembly, a collimating mirror M1 in the collimating mirror assembly, a customized blazed grating G1 in the grating assembly and a focusing mirror M2 in the focusing mirror assembly are sequentially arranged in the light propagation direction. The customized blazed grating G1 is designed by adopting specific parameters including the scribed line density, the blazed wavelength, the notch groove depth and the groove width ratio, and primary light energy enhancement and secondary light intensity suppression can be realized; according to the invention, an optical filter is not needed, the diffraction efficiency of a monochromator is improved to 60-86%, the intensity ratio of luminol secondary light to primary light is less than or equal to 1.6%, the detection limit of ruthenium bipyridine is reduced from 100pmol / L to 50pmol / L, and the electrochemical luminescence detection device is suitable for various electrochemical luminescence detection scenes.

The invention belongs to the technical field of biosensors, and provides a tobacco mosaic virus (TMV) dual-mode biosensor as well as a preparation method and application thereof. The biosensor is composed of a dual-mode luminescent material Co / Ce-TCBPE (at) Ru (dcbpy) 32-COOH, a lateral flow chromatography LFIA flexible electrode, a TMV antibody Ab1, a TMV antibody Ab1 and a self-assembled micro electrochemical luminescence (ECL) sensor, and through qualitative and quantitative dual-signal output of vision and ECL detection, the TMV detection sensitivity and accuracy are remarkably improved. The detection range of the sensor is 0.1 pg / mL-100 ng / mL, the detection limit reaches 48.9 fg / mL, and the sensor has the advantages of simplicity and convenience in operation, quick response, high stability, dual signal guarantee and the like, is suitable for quick visual detection of plant diseases, and has a wide application prospect in agricultural detection.

The invention relates to the technical field of organic detection, in particular to a nanoparticle modified quantum dot MOF material and a preparation method and application thereof. A zirconium-based double-ligand metal organic framework material is used as a carrier, a co-reaction accelerant Ti3C2 quantum dot is modified to enhance the electrochemical luminescence reaction efficiency, and copper-gold nanoparticles are loaded to amplify signals. The nanoparticle modified quantum dot MOF material provided by the invention can be prepared into an aptamer biosensor for accurately identifying malathion, and has high sensitivity and high selectivity. According to the invention, a nanoparticle modified quantum dot MOF material is coupled and combined with an aptamer, and an aptamer probe with high electrochemical luminescence activity and specific sensitivity to malathion is constructed. When the malathion acts on the aptamer probe, the malathion is combined with the aptamer in a high affinity manner, the aptamer probe is separated from the surface of the electrode, and meanwhile, an electrochemical luminescence signal is changed, so that the malathion residue in the agricultural product is rapidly detected.

The invention belongs to the field of functional nano materials and optical imaging, and particularly relates to a gold nanocluster and a preparation method and application thereof. The surface of the gold nanocluster contains a sulfydryl ligand A and a sulfydryl ligand B, the sulfydryl ligand A is ATT, and the sulfydryl ligand B is a sulfydryl ligand containing arginine; fluorescence quantum efficiency and electron transfer efficiency are synergistically enhanced on the basis of interaction of hydrogen bonds in the gold nanoclusters, the ultra-small luminous gold nanoclusters protected by double ligands are synthesized, the electrochemical luminescence quantum efficiency of the ultra-small luminous gold nanoclusters is up to 13.0% and is 4.2 times of that of a standard compound terpyridyl ruthenium in the field of electrochemical luminescence, and the ultra-small luminous gold nanoclusters are high in electrochemical luminescence quantum efficiency. An electrochemical luminescence transition area can be narrowed by 10 times compared with a terpyridyl ruthenium imaging system, and zero-thickness ECL luminescent layer cell high-resolution imaging can be realized; good application prospects are realized in the fields of fluorescence detection, electrochemical luminescence detection, fluorescence imaging, electrochemical luminescence imaging and the like.

The invention discloses an immunosensor for detecting Alzheimer's disease neurofilament light chain protein based on a two-dimensional metal organic framework and a preparation method of the immunosensor, and belongs to the technical field of biological detection sensors. An electrode modified with a RuMOFNSs-Ab2 conjugate and an Au-coated MoS2-Ab1 conjugate is used as a working electrode; the sandwich type electrochemical luminescence immunosensor is based on the two-dimensional RuMOFNSs / Au-coated MoS2, can be used for detecting the blood marker NfL of the Alzheimer's disease, provides a thought for the field of analysis and detection of the NfL, and also provides a reference for clinical medicine detection.

A method for electrochemiluminescence detection of hydroquinone based on aggregation-induced emission Cd-MOF is provided, including: step 1, preparation of Cd-MOF material; step 2, mixing Cd-MOF material with co-reactant; and step 3, establishment and application of hydroquinone sensor. The organic ligand TPPE with aggregation-induced emission effect and CdCl2 are used to prepare self-luminous Cd-based metal-organic framework Cd-MOF with rigid and directional structure through coordination.

The application relates to a sensor for synchronously detecting enniatin and roquefortine based on a double-potential electrochemiluminescence immunoassay technology as well as a preparation method and a detection method thereof. The sensor comprises a base electrode, a sensing interface composed of a nanocomposite is modified on the surface of the base electrode, mixed coated antigens including ENNs-BSA and AOH-BSA fixed on the sensing interface, mixed probes including a cathode probe and an anode probe, the cathode probe is Zr-Por-MOF-ENNs Ab which is obtained by marking ENNs antibodies with a porphyrin zirconium metal organic framework, the anode probe is Ce-In-MOF@ABEI-AOH Ab which is obtained by marking AOH antibodies with ABEI loaded on a cerium-doped indium metal organic framework, and a detection solution containing a single co-reactant potassium persulfate. The application can realize one-time sample adding, double-potential scanning and synchronous quantification for two kinds of mycotoxins of enniatin and roquefortine.

This invention belongs to the field of biosensing and detection technology, specifically disclosing a Tau protein electrochemiluminescent aptamer sensor, its preparation method, and its application. The sensor uses a CsPbBr₃@PVP / Au composite material as the electrochemiluminescent substrate. A Tau protein-specific aptamer is immobilized on the substrate surface via Au-S bonds, and non-specific adsorption sites are blocked with 6-mercapto-1-hexanol. The CsPbBr₃@PVP / Au composite material is prepared by encapsulating CsPbBr₃ with PVP and then combining it with Au NPs. This invention utilizes the coordination passivation effect of PVP and CsPbBr₃ to significantly improve the aqueous stability and electrochemiluminescence efficiency of perovskite nanocrystals. Combined with the anchoring effect of AuNPs and the specific recognition effect of aptamers, it achieves ultrasensitive detection of Tau protein with a detection limit as low as 0.80 fg / mL. Furthermore, it exhibits excellent selectivity, stability, and detection accuracy in complex biological samples such as cerebrospinal fluid, providing a novel and efficient detection platform for the early diagnosis of Alzheimer's disease and showing promising clinical application prospects.

The application relates to a method for enhancing electrochemiluminescence signals by ion-pi interaction, belonging to the fields of electrochemiluminescence and analytical chemistry. 2+ By modifying the working electrode with a conjugated pi system, cation-pi interaction is introduced into the Ru(bpy)3 / triethylamine (TEA) system, efficient enrichment of TEA protonated cations near the electrode is realized, thereby greatly enhancing the electrochemical reaction rate and ECL signal. Further, anion-pi interaction is utilized to realize ECL signal enhancement of the luminol ECL system. In addition, the conjugated pi system is exposed to the outermost boundary of the electrode, so as to provide effective ion-pi interaction for ECL; when the pi system is covered by an alkyl chain, the modified electrode can only exhibit hydrophobic interaction. The method disclosed by the application provides a new strategy for extending ion-pi interaction to all types of ECL systems and constructing a high-sensitivity ECL analysis platform.

This invention relates to a dual-potential electrochemiluminescence immunosensor based on enzymatic in-situ generation of a co-reactant, its preparation method, and its application. The sensor includes: a substrate electrode; a GOx@Au@MoS2 multifunctional sensing interface; a hybrid probe consisting of a cathode probe and an anode probe; the cathode probe is a Py-HOF-labeled AFB1 antibody, and the anode probe is a UiO66-NH2@luminol-labeled OTA antibody; glucose is added to the detection solution, and hydrogen peroxide is generated in situ through immobilized glucose oxidase, serving as the single co-reactant for both the cathode and anode. During detection, this invention generates H2O2 in situ through enzymatic generation, simultaneously exciting ECL signals at both the cathode and anode; it solves problems such as poor stability of the H2O2 co-reactant, inconvenient concentration control, multiple operation steps, and insufficient continuous supply; and it enables simultaneous quantitative detection of AFB1 and OTA with a single sample addition, without the need for external H2O2.

The invention discloses construction and application of a biosensor based on an efficient antifouling interface and dual signal amplification, and belongs to the technical fields of optical / electric analytical chemistry, biosensing and material science. According to the invention, the electrode interface is modified with polyaniline doped with polytannic acid as an antifouling film, so that non-specific adsorption of interfering biomolecules in a complex medium is resisted; euTb-MOF is prepared as an efficient luminous body, Tb < 3 + > can transfer energy of Tb < 3 + > and ligand to Eu < 3 + >, and effective signal self-enhancement is realized; according to the EuTb-MOF, the reduction of S2O8 < 2-> is promoted by utilizing the reversible conversion of valence states of Co < 2 + > / Co < 3 + > and Fe < 2 + > / Fe < 3 + > and the synergistic catalysis of Ag NPs, so that the electrochemical luminescence signal of the EuTb-MOF is further improved. Based on the above strategy, the constructed biosensor has high detection sensitivity and precision and long service life, the linear range is 10 fM to 100 nM, and the detection limit is as low as 3.62 fM.

The application relates to the field of electrochemiluminescence detection, in particular to a preparation method and a detection method of an electrochemiluminescence sensor for detecting di(2-ethylhexyl) phthalate. Technical points are as follows: the electrochemiluminescence aptamer sensor is formed by loading carboxylated ligands on the surface of a Zr-MOF / GDY composite material modified glassy carbon electrode; the Zr-MOF / GDY composite material is formed by electrostatic interaction between Zr-MOF and GDY; and the carboxylated aptamer is an aptamer containing a base sequence of 5'-GGGTAGGGCGGGAAGTTACTGTCTTACTGTC GTA-3'. The recovery effect of DEHP on the ECL signal intensity of the electrochemiluminescence aptamer sensor of the GDY and Zr-MOF composite material is used to realize the detection of DEHP, and the detection method is simple in operation, good in selectivity and high in sensitivity.

The application belongs to the technical field of electrochemical analysis and detection, and discloses a preparation method and application of an electrochemiluminescence sensor for specifically detecting trilobatin. The specific operation includes: (1) preparing a composite material of nitrogen-doped carbon quantum dots (N-CDs) and a silver-based metal organic framework (AgMOF) (AgMOF@N-CDs); (2) modifying the AgMOF@N-CDs on a glassy carbon electrode to obtain AgMOF@N-CDs / GCE; and (3) based on a three-electrode system, using the AgMOF@N-CDs / GCE as a working electrode, a platinum wire as an auxiliary electrode, and Ag / AgCl as a reference electrode to construct an electrochemiluminescence sensor for detecting trilobatin. The preparation of the electrochemiluminescence sensor is simple, the analysis speed is relatively fast, the detection range is relatively wide, the sensitivity is relatively high, and the selectivity is relatively good.

Disclosed herein are formulations, substrates, and arrays. Also disclosed herein are methods for manufacturing and using the formulations, substrates, and arrays. Also disclosed are methods for identifying peptide sequences useful for diagnosis and treatment of disorders, and methods for using the peptide sequences for diagnosis and treatment of disorders, e.g., celiac disorder. In certain embodiments, substrates and arrays comprise a porous layer for synthesis and attachment of polymers or biomolecules.

The invention relates to the technical field of electrochemical luminescence sensing, and discloses an electrochemical luminescence immunosensor for detecting interleukin-1 beta, a preparation method and application thereof, the electrochemical luminescence immunosensor comprises a working electrode, a reference electrode and a counter electrode; the working electrode comprises a substrate electrode and an ordered mesoporous silica film (SNF) modified on the surface of the substrate electrode; a composite nano-enzyme containing cobaltosic oxide nanoparticles and nitrogen-doped graphene quantum dots is loaded in a nano-pore channel of the SNF, the outer surface of the SNF is modified with an epoxy group, and an anti-interleukin-1beta antibody is covalently immobilized through the epoxy group; a detection system of the electrochemical luminescence immunosensor is a luminol / dissolved oxygen system. According to the electrochemical luminescence immunosensor, a luminol / dissolved oxygen system signal can be remarkably enhanced, and high-sensitivity and accurate quantitative detection of interleukin-1 beta is realized under a neutral condition.

The invention provides a wireless photochargeable electrochemical luminescence system and device and preparation and application thereof, the system comprises cyano-functionalized CN-K. The device comprises a detachable top camera obscura, a bottom unit, a reactor (containing a functionalized carbon nitride solution integrating solar energy conversion and storage and electrochemical luminescence capability) and a signal acquisition unit. Functionalized carbon nitride is utilized to realize an integrated process of photocharging, electron storage and self-luminescence: the material firstly absorbs sunlight to accumulate long-life electrons, then the accumulated electrons are used as a unique driving force to trigger a surface electrochemical luminescence reaction to realize self-luminescence, and the whole process can be monitored in real time by a signal acquisition unit; a detection signal can be read in real time through equipment such as a smart phone. The wireless self-driven electrochemical light-emitting device has the potential of multi-scene on-site instant detection, and can be applied to visual rapid screening of pollutants of outdoor water.

The invention discloses construction and application of a biosensor for triggering controlled release based on adjacent induced DNA strand displacement, and belongs to the technical fields of electrochemical luminescence, biological analysis and nano science. According to the invention, by integrating adjacent induced DNA strand displacement and controlled release strategies, the ultra-sensitive self-closing dual-mode biosensor taking the Au-Ag nanocluster as a luminous body is developed, and the ultra-sensitive self-closing dual-mode biosensor is used for trace detection of neuron-specific enolase. Due to the synergistic characteristic between the double metals, the Au-Ag nanocluster has high electrochemical luminescence (ECL) efficiency. Meanwhile, the reduction of S2O8 < 2-> is catalyzed by cyclic conversion between Fe < 2 + > / Mn < 3 + > and Fe < 3 + > / Mn < 4 + >, and a large amount of SO4 <-> is generated for ECL emission. The specific binding between the target antigen and the antibody can drive DNA strand displacement to open a blocked hole, so that the electrochemical probe falls off and the probe is quenched to release, the self-closing of an electrochemical signal and an ECL signal is realized, the detection sensitivity and accuracy of the sensor are effectively improved, and the sensor is suitable for immunoassay of various biomarkers.

This invention belongs to the field of microbial electrochemistry and single-cell analysis technology, and specifically relates to a femtocoulomb-level single-bacterial surface charge imaging method and its application. This technology is based on electrochemiluminescence microscopy, utilizing the spontaneous enrichment of cationic ECL luminescent molecules by the electrostatic field of the negative charge on the bacterial surface, thereby non-destructively enhancing its ECL signal. By establishing a quantitative relationship between the ECL intensity enhancement ratio and the number of adsorbed molecules, and combining this with COMSOL simulation to determine the thickness of the luminescent layer, it achieves for the first time high-throughput, high spatiotemporal resolution dynamic quantitative measurement of femtocoulomb-level charges on the surface of a single living bacterium. The invented technology has high sensitivity, high resolution, and good biocompatibility, and can be used to reveal bacterial metabolic-charge regulation mechanisms, analyze synergistic electron transport pathways, and screen highly electroactive bacterial subpopulations in situ, providing a key tool for microbial electrochemical research.

This invention develops a pH-responsive controlled-release electrochemiluminescence sensor based on glucose oxidation and its application in the detection of CYFRA 21-1, belonging to the field of electrochemiluminescence sensor construction technology. The sensor constructed in this invention uses BSA / luminol-Ab2 / SiO2-PEI@Au NPs as the secondary antibody label and CeO2-Au as the substrate material. It utilizes the oxidation of glucose to gluconic acid under voltage, thereby lowering the pH. The sensor constructed in this invention has a wide detection range, high sensitivity, and low detection limit, providing a feasible solution for the detection of CYFRA 21-1.

To provide a small, easy-to-operate electrochemiluminescence sensor. [Solution] An electrochemiluminescence sensor for detecting a test substance in a sample using nanoparticles with an electrochemiluminescence immunoassay method utilizing a plasmon-enhanced field, comprising: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; the nanoparticles comprising: metal nanoparticles; a polymer film covering the surface of the metal nanoparticles; a second specific binding substance bound to at least one of the polymer film and the metal nanoparticles and specifically binding to the test substance; and an electrochemiluminescent substance bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant, wherein the electrochemiluminescence sensor comprises: a first substrate; an electrode provided on the surface of the first substrate, wherein a first specific binding substance that specifically binds to the test substance is bound to the surface of the electrode; and an electrochemiluminescent substance that is bound to at least one of the polymer film and the second specific binding substance and capable of contacting a co-reactant.

The invention belongs to the technical field of luminescence imaging detection, and particularly relates to an electrochemical luminescence imaging detection platform based on a closed bipolar electrode array and application. Comprising a sensor and a detection unit, a bipolar electrode array unit comprises an insulating substrate, an anode region and a cathode region, the anode region and the cathode region are isolated from each other and are of the same structure, and each anode region or each cathode region is provided with a plurality of independent holes which are arrayed in parallel; gold nanoparticles are loaded in a plurality of independent open pores of the parallel array in the anode region, and a Ru (bpy) 32 < + >-TPA system is constructed; au / g-C3N4 composite materials are loaded in a plurality of independent open pores of the parallel array in the cathode region, and an Au / g-C3N4-K2S2O8 system is constructed. According to the invention, based on the bipolar electrode array unit, the terpyridyl ruthenium and the gold / graphite phase carbon nitride are used as different illuminants to realize capture and quantitative analysis for synchronous detection of acetaminophen and rutin, and the detection accuracy and high-throughput analysis are ensured.