491 results about "Electrochemiluminescence" patented technology

The invention discloses a construction method and application of an electrochemical luminescence (ECL) antifouling sensor based on zwitter-ion hydrogel, and belongs to the technical fields of photoelectric sensing analysis, interface antifouling and environmental science. According to the invention, the hydrogel based on sulfobetaine methacrylate zwitterions and bovine serum albumin is used as the antifouling coating, so that the adsorption of biological proteins and bacteria is effectively hindered, and the service life of the sensor is prolonged. Meanwhile, the interior label Au (at) luminol is packaged in the antifouling hydrogel, so that the integration of interface antifouling and ratio detection is realized, the detection accuracy is improved, and the construction process of the ECL sensor is simplified. The constructed ECL anti-pollution sensor has high sensitivity to chloramphenicol detection, the detection limit is 0.39 pM, and the ECL anti-pollution sensor is suitable for trace detection of antibiotics in seawater.

The present application discloses a dry electrochemiluminescent lateral flow immunoassay strip and its application in immunoassay, wherein the dry electrochemiluminescent lateral flow immunoassay strip comprises a lateral flow immunoassay strip, an electrode pad and a connection pad; the lateral flow immunoassay strip is composed of a sample pad, a binding pad, a detection pad with a backing NC membrane and an absorption pad stacked on a transparent adhesive plate; the electrode pad comprises an integrated closed bipolar electrode and a driving electrode; the integrated closed bipolar electrode comprises a closed bipolar electrode detection anode and a quality control anode and a shared cathode of the two anodes; the lateral flow immunoassay strip is inverted on the electrode pad; the T line and C line on the detection pad are in contact with the detection anode and the quality control anode on the electrode pad, respectively. The present invention adopts T / C for quantitative detection of biomarkers in ECL lateral flow immunoassay for the first time, solves the problem that the quality control signal is not fully utilized in the existing ECL lateral flow immunoassay, and improves the detection stability and storage stability.

The invention discloses a high-sensitivity cardiac troponin-T electrochemiluminescence detection kit. The kit comprises a magnetic microsphere reagent coated by a high-sensitivity cardiac troponin-T capture antibody and a high-sensitivity cardiac troponin-T detection antibody reagent labeled by an electrically neutral ruthenium compound, the structural general formula of the electrically neutral ruthenium compound is # imgabs0 # n = 0-9; the magnetic microsphere reagent coated with the high-sensitivity cardiac troponin-T capture antibody comprises a magnetic microsphere coated with the high-sensitivity cardiac troponin-T capture antibody and a diluent; the electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody reagent comprises an electrically neutral ruthenium compound labeled high-sensitivity cardiac troponin-T detection antibody and a diluent; the diluent is prepared from piperazine-1, 4-diethyl sulfonic acid or 3-(N-morpholinyl)-2-hydroxy propanesulfonic acid. When the high-sensitivity cardiac troponin-T electrochemiluminescence detection kit is used for detection, a reaction system does not contain streptavidin coated magnetic beads or biotinylated antibodies, so that interference of biotin in a sample is avoided.

The utility model discloses an electrochemical luminescence lateral flow gene detection reagent strip and a kit, the electrochemical luminescence lateral flow gene detection reagent strip comprises an electrode and two hydrophilic channels A and B, and the electrode and the two hydrophilic channels are partially overlapped; the hydrophilic channels A and B are distributed on the sample adding sheet, the combination sheet, the detection sheet and the absorption sheet; the electrodes comprise an integrated bipolar electrode and a pair of driving electrodes; the integrated bipolar electrode comprises a cathode and at least two anodes which are connected in parallel; wherein the integrated bipolar electrode anode close to the sample adding sheet is called a quality control anode, and the integrated bipolar electrode anode close to the absorption sheet is called a detection anode. The electrode plate of the detection reagent strip is a closed bipolar electrode, and the anodes of the two bipolar electrodes are symmetrically distributed in parallel, so that the area of the electrode is reduced, the occupied space of the electrode is reduced, a plurality of detection anodes can be arranged, and the detection reagent strip has the potential of multi-path multi-element detection.

The invention belongs to the field of electrochemical sensing, and particularly relates to construction and application of an electrochemical luminescence sensor for detecting the content of N-nitrosodimethylamine with high sensitivity. The Cas12a enzyme improves the specificity and realizes signal amplification through the recognition mechanism and trans-cleavage capability of the Cas12a enzyme. The ATT-AuNCs is used as an efficient electrochemical luminescence emitter, so that the transition of a signal is promoted. After a sample is added, the Cas12a-crRNA compound can specifically recognize a DNA activator to trigger cutting of non-specific DNA which is modified on a glassy carbon electrode and is marked by ferrocene, so that the electrochemical luminescence intensity is increased. The NDMA in the sample can be detected through the method, so that the content of nitrosamine in food can be detected. The CRISPR / Cas12a is combined with an electrochemical luminescence technology, so that a sensitive and rapid NDMA detection means is provided, and a new idea is provided for detecting the content of cancerogenic substances in various foods.

The invention discloses an immunosensor for detecting cytokine interferon gamma through electrochemiluminescence as well as a preparation method and application of the immunosensor. The immunosensor comprises a three-electrode system consisting of an immunoelectrode, a reference electrode and a counter electrode, the immunoelectrode is a VMSF modified electrode confined to cerium dioxide nano enzyme and platinum nano particles, and is obtained by covalently immobilizing an IFN-gamma antibody and closing a non-specific site; when the immunosensor is used for detection, a solution containing luminol is used as an electrolyte. The CeO2 and the PtNPs which are confined in the nano channel show a remarkable synergistic catalysis effect. Compared with direct modification on a plate electrode, CeO2-PtNPs has higher catalytic activity and stability. The sensor provided by the invention is used for INF-gamma detection, has the advantages of high sensitivity, low detection limit and wide detection range, does not need complex pretreatment and separation processes during detection, reduces the detection cost, is simple to operate, and is suitable for large-scale application.

The invention belongs to the technical field of immunoassay and biosensing detection, and particularly relates to a preparation method and application of an antibiotic electrochemical luminescence sensor based on a double-enhancement strategy. According to the invention, a bifunctional metal organic gel is used as a substrate, a graphite-phase carbon nitride quantum dot is used as a luminous body, and a self-assembly split aptamer walker is combined to construct the electrochemical luminescence sensor for sensitive detection of antibiotics. The sensor has the advantages of extremely high specificity and sensitivity, wide detection range, low detection limit and strong anti-interference performance, and has important scientific significance and application value in detection of antibiotic residues in the environment.

The invention discloses a construction method and application of a ratio-dependent electrochemical luminescence (ECL) biosensor based on an antifouling red cell membrane, and belongs to the technical fields of photoelectric analysis, biosensing and interface antifouling. In the invention, the erythrocyte membrane with super-hydrophilicity and low membrane protein content is used as an efficient antifouling interface, and the good biocompatibility of the erythrocyte membrane avoids the influence of a chemical antifouling coating on the biological activity of the ECL sensor. Meanwhile, the electropolymerized polyaniline nanowire is embedded into the antifouling erythrocyte membrane, so that the chemical stability and the conductivity of the antifouling erythrocyte membrane are enhanced, and the application of the antifouling erythrocyte membrane in ECL analysis is expanded. C3N4 is introduced as an interior label for eliminating background signals and external interference, so that the detection precision of the biosensor is improved. On the basis, the constructed ECL antifouling biosensor realizes ultra-sensitive detection of human breast cancer cells MCF-7 in human serum, and the detection limit is as low as 3 cells / mL.

The invention provides an electrochemical luminescence MeHA microneedle patch and a preparation method and application thereof.The preparation method comprises the steps that hyaluronic acid is modified into methacrylated hyaluronic acid, MeHA solid powder is obtained after purification, and the MeHA solid powder is mixed with a photoinitiator to form a precursor solution; and pouring into a microneedle mold, centrifuging, drying and carrying out UV crosslinking to obtain the MeHA microneedle patch loaded with ABEI and glucose oxidase. Through the MeHA microneedle patch-SPE electrode integrated design, the integrated operation of skin interstitial fluid in-situ extraction-enzymatic reaction-electrochemical luminescence detection is realized, sample transfer is not needed, the interference of intermediate links is greatly reduced, the detection accuracy and efficiency are remarkably improved, and the device and the method are particularly suitable for clinical real-time monitoring scenes.

The invention relates to a preparation method of a microwave-assisted synthesized near-infrared electrochemical luminescence gold nanocluster and application of the gold nanocluster in miRNA (micro Ribonucleic Acid) detection. The preparation method comprises the following steps: under a stirring condition, adding a gold source and alkali into a Met solution to obtain a mixed solution, carrying out microwave irradiation reaction, and carrying out solid-liquid separation to obtain a Met-Au NCs solution; the preparation method comprises the following steps: activating a Met-Au NCs solution by using EDC and NHS, adding a DNA solution, mixing, and dialyzing to obtain a Met-Au NCs-DNA compound, namely the near-infrared electrochemical luminescence gold nanocluster. Compared with Au NCs prepared by a common mild method, the Met-stable Au NCs prepared by a microwave-assisted method has the advantage that the fluorescence and electrochemical luminescence efficiency at 835 nm is improved. The invention also provides a'signal closing 'ECL (electron cyclotron ligand) biosensor for high-sensitivity detection of the miRNA-107 (MicroRNA-107), and a method for detecting the miRNA-107 (MicroRNA-107). The sensor is simple to operate and good in repeatability, and has important scientific significance and application value for clinical early diagnosis of cancers.

The invention relates to a paper-based electrochemiluminescence novel coronavirus antigen detection test strip as well as a preparation method and application thereof. The test strip comprises a bottom plate, and a paper-based channel layer and an electrode layer are arranged on the bottom plate; the paper-based channel layer comprises a sample pad, a combination pad, a detection pad and a water absorption pad which are sequentially overlapped; the combination pad is coated with an SARS-CoV-2 first antibody marked by ruthenium coupling; the detection pad is coated with an SARS-CoV-2 second antibody; the recognition antigen epitopes of the SARS-CoV-2 first antibody and the SARS-CoV-2 second antibody marked by ruthenium coupling are different; the electrode layer is provided with a plurality of electrodes, and one end of each electrode is connected with the detection pad. Aiming at SARS-CoV-2 detection, a lateral flow immunochromatography technology and an electrochemical luminescence technology are combined, a brand-new detection test strip is designed, and low-cost, rapid and high-sensitivity detection is realized.

The invention relates to the field of electrochemiluminescence imaging analysis, and particularly discloses a single-particle electrochemiluminescence imaging method by adjusting the thickness of a molecular self-assembly layer, which is used for solving the problems of serious non-specific luminescence of an electrode substrate, incapability of finely regulating an interface structure, poor stability and the like in single-particle electrochemiluminescence imaging. The method comprises the following steps: preparing an insulating molecule self-assembly layer with gradient thickness, optimizing a fixing process of gold nanoparticles, determining the position of a single particle, carrying out ECL imaging acquisition on the single particle, and optimizing the thickness of the molecule self-assembly layer. According to the method, the thickness of a molecular self-assembly layer is precisely regulated and controlled, a nano-particle fixing and ECL system is optimized, and a single-particle ECL imaging device is built, so that the signal-to-noise ratio of single-particle electrochemical imaging can be increased, and background interference caused by non-specific luminescence of an electrode substrate is effectively inhibited.

The application discloses an electrochemiluminescence immunosensor for detecting interleukin-1 beta and a preparation method and application thereof, and comprises a three-electrode system of a working electrode, a reference electrode and a counter electrode; the working electrode is a modified electrode of CoAl layered double hydroxide, is loaded with platinum nanoparticles after being etched by alkali, and is obtained by fixing interleukin-1 beta antibodies and blocking non-specific sites; and a solution containing luminol is used as an electrolyte during detection of the immunosensor. The working electrode of the immunosensor takes oxygen-rich vacancy CoAl layered double hydroxide as a substrate, combines with platinum nanoparticles to realize EMSI effect, significantly enhances the electrochemiluminescence signal of a luminol-dissolved oxygen system, realizes quantitative detection of IL-1 beta by combining with specific antigen-antibody recognition, and has the characteristics of strong anti-interference ability, fast detection speed and good stability.

The invention relates to the field of sensors, and discloses a solid electrolyte for ammonia gas detection, an electrochemical luminescence sensor, a preparation method and a detection method, and the solid electrolyte for ammonia gas detection comprises the following raw materials: a second electrolyte, a luminescent substance, an optional first electrolyte and an optional binder. The solid electrolyte for ammonia gas detection provided by the invention can realize ppb-level NH3 selective detection with high sensitivity, wide linear response range and low detection limit at room temperature for an electrochemical luminescence sensor for detecting ammonia gas. The electrochemical luminescence sensor for detecting the ammonia gas can be used for sensing the ammonia gas through a cyclic voltammetry method and a constant potential-luminescence technology, and ppb-level high-sensitivity and high-selectivity ammonia gas detection is realized.

The invention discloses construction and application of a confinement enhanced photoelectrochemistry-electrochemiluminescence dual-mode biosensor, and relates to the technical field of biosensing and analytical chemistry. The preparation method of the biosensor comprises the following steps: taking a CdS (at) SiO2 (at) NaYF4: Yb / Tm nano composite material as a substrate to obtain a confinement enhanced photoelectrochemistry (PEC) and electrochemiluminescence (ECL) sensing interface; in combination with a DNA wheel nanostructure triggered by target acetamiprid, signal amplification is realized; and synchronous dual-mode quenching detection of PEC and ECL signals is realized by virtue of peroxidase-like catalytic precipitation and wide-spectrum absorption characteristics of the PtPd-CoSnO3 nanocube. The biosensor prepared by the invention has the advantages of high sensitivity, strong reliability, good selectivity and the like, can be applied to accurate detection of acetamiprid in samples such as agricultural products and the like, and has important application value in the aspects of environmental monitoring and food safety control.

The invention relates to the technical field of electrochemical luminescence detection, in particular to an electrochemical luminescence aptamer sensor based on a CoOOH-cDNA / Apt / AgNWs / Tb (at) A-COF / GCE working electrode. The iBF in a sample to be detected is detected by utilizing LC-MS / MS, GC-MS, UHPLC and HPLC-MS, so that the cost is high, the speed is low, and the sensitivity is relatively low. Aiming at the technical problem, the invention provides the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE working electrode, the working electrode takes Tb-coated A-COF as a donor and CoOOH NSs as a receptor, the donor and the receptor are combined and modified on a glassy carbon electrode (GCE) through an aptamer and cDNA, then the CoOOH-cDNA / Apt / AgNWs / Tb-coated A-COF / GCE is taken as the working electrode, and iBF in a sample to be detected is quantitatively detected. According to the invention, through a signal "on-off-on" (on-off-on) mode, the consistency of detection results is significantly improved, the generation of false positive or false negative signals is reduced, and the detection accuracy is improved.

The invention discloses an electrochemical test performed by preparing a novel electrochemical luminescence material and application of the electrochemical luminescence material to an electrochemical luminescence sensor. The invention designs an electrochemical luminescence sensor for detecting carbaryl based on a rare earth metal Eu co-reaction-free promoter complex illuminant. Specifically, the electrochemical luminescence sensor is prepared by taking a complex of rare earth metal europium as a luminescent material and potassium persulfate as a co-reactant. The sensor shows an excellent development prospect in the aspect of carbaryl detection.

The present invention is directed to assay methods and compounds and kits used in such assays.

The invention discloses a biosensor and a method for detecting NF-kB p50, the biosensor comprises: an electrode, the surface of which is bound with a DNA tetrahedral structure; the DNA tetrahedral structure is used for being combined with a detection probe DNA; the detection probe DNA comprises a detection probe DNA nucleic acid sequence and an electrochemical luminescence group connected with the detection probe DNA nucleic acid sequence. The biosensor can realize reversible dissociation of detection probe DNA under an alkaline condition, so that at least 10 times of repeated detection cycles are supported, and the detection sensitivity and specificity are not influenced. According to the detection method, a differential pulse voltammetry is adopted, a collateral cleavage signal amplification strategy of a CRISPR-Cas12a system is combined, high-sensitivity detection of NF-kappa B p50 is achieved, the method is suitable for complex biological samples and has high sensitivity, high specificity and good reuse performance, the lowest detection limit can reach the 100 fly mole level, and the linear detection range covers 100 aM to 80000 aM.

The invention discloses a preparation method and application of an In2O3-Co3O4 heterostructure material, an MOFs-MOFs heterojunction takes In-MOFs as a subject and ZIF-67 as an object, and epitaxial growth of an MOFs structure is realized. And the sensor is applied to ultra-sensitive gas sensing detection, so that acetoin detection in a low-power-consumption mode is realized. The constructed sensor has the characteristics of high sensitivity, high response and recovery speed, good selectivity and the like. The unique MOFs-MOFs structure increases the adsorption active sites of the composite material, and is beneficial to the improvement of the gas sensing performance. The successful preparation of the material provides a new strategy for reasonable preparation of MOFs derivatives. Technical reference is also provided for related chemical sensors such as electrochemiluminescence sensors and electrochemical sensors, and the method has wide potential use value.

The invention discloses a full-automatic electrochemical luminescence detector based on a photodiode, and relates to an electrochemical luminescence detector, the full-automatic electrochemical luminescence detector comprises a shell, the shell is internally provided with an optical signal acquisition module, a small packaging circuit board, an ECL chip, a man-machine interaction module and a chip inlet and outlet device; a light current detection unit, an electrochemical reaction excitation unit and a microcontroller are arranged in the small packaging circuit board; wherein a test strip sample pad and a reaction area are arranged in the ECL chip. The photodiode used in the invention can make a quick response to the change of light intensity, which is far faster than the frame capturing speed of the camera, and the demand and complexity of subsequent data processing are reduced, so that the system is easier to realize automation. Compared with a camera, the photodiode is lower in hardware cost, does not need to use complex image processing software, and is suitable for household use. The detector is compact in structure, small and exquisite in appearance and convenient to use in a handheld mode and carry about.

The invention discloses a dry-type electrochemical luminescence (ECL) lateral flow chip and application thereof in CRISPR-Cas detection. The dry-type ECL lateral flow chip comprises a lateral flow test strip and an electrode plate, the lateral flow test strip comprises more than one detection test strip, one electric connection test strip and at least one quality control test strip; the test strip sequentially comprises an activation sheet, a combination sheet, a detection sheet, an absorption sheet and a common sample sheet, the test strip forms radial branches taking the sample piece as the center; the electrode plate comprises an integrated closed bipolar electrode and a driving electrode; the shape of the integrated closed bipolar electrode is the same as that of the lateral flow test strip. According to the invention, the CRISPR-Cas system is freeze-dried on the test strip, so that accurate multiple detection of pathogens can be realized; according to the method, the operation process is simplified, the detection time is greatly shortened, the operation error is reduced, the mutual interference among multiple detections is reduced, and the generation of false positive results is avoided.

The invention belongs to the technical field of immunodetection, and particularly relates to a triiodothyronine chemiluminescence immunodetection kit as well as a preparation method and application thereof. According to the triiodothyronine chemiluminescence immunodetection kit provided by the invention, FT3 / T3 is detected on an electrochemical luminescence platform by adopting a double-antibody sandwich method for the first time, so that the detection sensitivity is effectively improved, the linear range of detection is increased, and the repeatability of detection is improved.

The invention relates to the technical field of electrochemical luminescence detection application, and discloses a single molecule detection method based on AI and electrochemical luminescence, and the method comprises the following steps: collecting electrochemical luminescence signal data of a sample to generate a data set, and receiving real-time signals to construct a matrix; processing the signal data matrix, extracting features in combination with the data set, predicting single molecule distribution according to the signal intensity gradient and the detection path information, outputting space-time distribution features through an AI model, and updating the data set; detection parameters such as sensor sensitivity, light source intensity and sampling frequency are dynamically adjusted according to distribution characteristics. According to the method, through combination of AI and electrochemical luminescence, determination of single molecule detection characteristics and dynamic adjustment of detection parameters are realized, and the accuracy and efficiency of single molecule detection are improved.

The invention belongs to the technical field of electrochemical luminescence detection equipment, and particularly relates to a microneedle patch for wearable electrochemical luminescence sensing equipment and application of the microneedle patch. The microneedle patch comprises a microneedle base and a microneedle array, the microneedle base is divided into a left area, a middle area and a right area through two parallel grooves; the middle area is conductive and light-transmitting, and the left and right areas are conductive and light-proof; the microneedle arrays are arranged in the three areas and are all subjected to conductive treatment; the three areas are separated by grooves and are insulated from one another and are not conductive; and the surface in each area has conductive performance, so that electric signals can be conducted. The light-emitting sensing equipment takes the microneedle patch as a real-time acquisition tool of a tissue fluid sample, detects the concentration of cTnI in the tissue fluid on line, realizes real-time monitoring of a marker cTnI of myocardial injury and acute myocardial infarction in vivo, has the characteristics of high detection sensitivity, portability, simplicity in operation, low cost and the like, and realizes online in-situ monitoring of wearable electrochemical luminescence of a patient.

The invention discloses a construction method and application of an electrochemical luminescence (ECL) biosensor based on a cell membrane hydrogel antifouling interface, and belongs to the technical fields of photo / electrochemical analysis, interface antifouling and biosensing. According to the invention, the cell membrane hydrogel is used as an efficient antifouling interface, so that non-specific adsorption of interfering proteins in a sample to be detected is hindered, and the service life of the biosensor is prolonged. Meanwhile, a luminophor copper nanosheet and a quencher MnO2 nanosheet are both introduced into the cell membrane hydrogel, in the presence of alkaline phosphatase (ALP), L-ascorbic acid-2-trisodium phosphate is catalyzed and hydrolyzed into ascorbic acid, and then MnO2 is reduced to recover ECL emission of the copper nanosheet. Therefore, the ECL signal of the biosensor is in a rising trend along with the rising of the ALP concentration. Based on this, the constructed ECL biosensor realizes sensitive detection of ALP, the linear range is 10 <-5 >-10 < 4 > U / L, and the detection limit is as low as 3.4 * 10 <-6 > U / L, which indicates that the ECL biosensor has potential application in clinical diagnosis.

The invention belongs to the technical field of biochemical detection, and particularly relates to a bionic microsphere, a non-diagnostic-purpose extracellular vesicle detection method and an extracellular vesicle detection kit, the bionic microsphere is prepared by modifying a homologous cell membrane CM on the surface of a silica microsphere MS; the bionic microsphere (CMS) is used for capturing specific types of extracellular vesicles (EVs) in a to-be-detected sample and an optical / electrochemical detection method, can specifically capture homologous EVs, and is combined with fluorescence, ultraviolet visible absorption, differential pulse voltammetry and electrochemical luminescence for detection.

The invention relates to a lanthanide oxide electrochemiluminescence aptamer sensor with dual signal amplification for detecting acetamiprid as well as a preparation method and application of the lanthanide oxide electrochemiluminescence aptamer sensor. According to the sensor, terbium-doped europium oxide (Eu2O3: Tb < 3 + >) modified by gold nanoparticles (Au NPs) is used as a luminous body, and the electrochemical luminescence performance can be remarkably improved through multiple mechanisms: on one hand, the Au NPs can effectively promote generation of more sulfate free radicals; on the other hand, the surface defect passivation effect and the efficient synergistic effect of energy transfer jointly achieve effective amplification of signals. In addition, the wool ball-shaped Ag NPs is used as a plasma resonance signal amplification source, so that the ECL intensity of the sensor is further enhanced. The prepared electrochemical luminescence sensor with the dual signal amplification effect is used for detecting acetamiprid (ACE), has a wide linear range and a low detection limit, and has a wide application prospect in detection of acetamiprid residues in a water environment.

The invention belongs to the technical field of electrochemical luminescence analysis and detection, and relates to an electrochemical luminescence sensor of a defective iron metal organic framework enhanced pyrenyl self-assembled hydrogen bond organic framework, and a preparation method and application thereof. Comprising the following steps: dispensing a PSA-HOF / Pca-MIL-88B (Fe) dispersion liquid on a GCE electrode, drying, dropwise adding a perfluorooctanoic acid aptamer, reacting at room temperature to finally obtain an aptamer modified PSA-HOF / Pca-MIL-88B (Fe) electrode as a working electrode, Ag / AgCl as a reference electrode and a Pt electrode as a counter electrode, and introducing the aptamer modified PSA-HOF / Pca-MIL-88B (Fe) electrode, Ag / AgCl as a reference electrode and a Pt electrode as a counter electrode into a PBS phosphate buffer solution containing potassium persulfate for detecting perfluorooctanoic acid. The prepared PSA-HOF / Pca-MIL-88B (Fe) system electrochemical luminescence sensor has the characteristics of simplicity, convenience and flexibility in operation, wide detection range, low detection limit, low detection cost and the like.

The invention belongs to the technical field of novel functional materials and biosensing detection, and provides a preparation method and application of a pyrenyl-based covalent organic framework immunosensor. Specifically, a pyrenyl covalent organic framework is adopted as an electrochemiluminescence material, and free rotation of 1, 3, 6, 8-tetra (4-formylphenyl) pyrene is effectively inhibited through connection of formylhydrazine, so that non-radiative transition is weakened, and the electrochemiluminescence signal intensity is enhanced. The nickel-iron-carbon nano tube loaded with the gold nano particles is used as a substrate material, on one hand, the three-dimensional interconnected carbon nano tube can improve the electron transfer efficiency, and the large-area gold nano particles can immobilize more antibodies, and on the other hand, the uniformly dispersed iron-nickel active center can promote the generation of more tripropylamine free radicals with strong oxidizing property, so that the anti-tumor effect is improved. The luminous efficiency of the pyrenyl covalent organic framework is further improved. The carbohydrate antigen 242 is detected according to different electrochemical luminescence signal intensities of to-be-detected substances with different concentrations.