This invention discloses a method and kit for detecting microbial SERS based on superhydrophobic
evaporation concentration and magnetically guided enrichment, belonging to the field of
in vitro diagnostics and
analytical chemistry. The method includes: mixing and incubating the sample to be tested with a magnetic SERS probe modified with targeted recognition molecules to form a complex; adding the mixed droplet to a superhydrophobic surface for
evaporation concentration; applying a
magnetic field below the detection site during
droplet evaporation, using the
magnetic gradient force to directionally enrich and pin the complex to the detection site, resulting in a dense
solid enrichment spot in situ after
drying, creating nanoscale gaps between the
metal layers of adjacent probes and exciting SERS hotspots; irradiating the spot with a
laser and acquiring the SERS
signal to achieve microbial detection. This invention also provides a kit for implementing this method, including a magnetic SERS probe, a superhydrophobic detection card, and a positioning base with an embedded
magnet. This invention eliminates the dependence on traditional
solid-phase SERS substrates, overcomes the defect of easy droplet rolling on superhydrophobic surfaces through magnetic pinning, achieves self-alignment and high-sensitivity detection, and has advantages such as strong anti-interference, low cost, and ease of
automation.