157 results about "Magnetic sphere" patented technology

A method and morphologically adaptable apparatus for altering the charge distribution upon living membranes with functional stabilization of the membrane physical electrical integrity further comprising a method for using quadripolar, circular, center charged, energy balanced magnetic device in a four (4) magnet array of alternating polarity in which the magnetic poles are in multiple planes and are separated by a predetermined distance which provide an effective magnetic sphere of influence on all adjacent poles to suppress the firing of action potentials of mammalian sensory neurons. The method and apparatus further provides a static magnetic device for production of a magnetic field for treatment of various disorders that can be focused at the site of pain or edema to deliver a gradient in the magnetic field to prevent or reduce charge flow. Further there is provided a static magnetic device for production of a magnetic field for treatment of disorders wherein the device provides a static magnetic field such that the focused magnetic field gradient is oriented To be perpendicular to the neuron or membrane charge flow providing maximum deflection of the ion or charge flow.

The invention relates to a synthetic method for a magnetic metal organic framework composite material coated by [Cu2(btc)2] on surfaces of ferroferric oxide microspheres and application of the composite material. The method comprises the following steps of: firstly synthesizing ferroferric oxide microspheres by a hydrothermal synthesis method; dispersing magnetic spheres in an ethanol liquid of mercaptoacetic acid, wherein hydroxyls are formed on the surface of the spheres; dispersing mercaptoacetic acid modified magnetic spheres to an ethanol liquid of copper acetate, reacting for 15 minutes at 70 DEG C, and then dispersing the product in an ethanol liquid of trimesic acid and reacting for 30 minutes at 70 DEG C; and performing alternate reaction of magnetic spheres with copper acetate and the ethanol liquid of trimesic acid to finally, obtain the magnetic metal organic framework composite material with a core-shell structure. The material has a metal organic framework shell layer and can be coordinated with peptide fragments with amino groups and carboxylic group so as to enrich low concentration peptide. Meanwhile, the enriching and separating process is fast, simple and convenient due to high paramagnetism of ferroferric oxide. The synthetic method is simple and low in cost, and can be used for enrichment and separation of low abundance peptide fragments less than 1nM and MALDI-TOFMS (Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer) detection.

The invention discloses a high-magnetic heavy-metal ion adsorbent carrying conductive high molecules and a preparation method thereof. The adsorbent comprises high-magnetic microspheres carrying conductive high molecules, each of which comprises a Fe3O4 polycrystal sphere cluster, an amorphous SiO2 protective layer and a polypyrrole adsorption outer layer from inside to outside. The preparation method of the adsorbent is realized in a way that: carrying out a solvent thermal reduction reaction on soluble ferrites to obtain the Fe3O4 magnetic sphere cluster; carrying out the basic hydrolysis by using tetraethoxysilane to obtain the Fe3O4-SiO2 nuclear shell sphere cluster; and finally, carrying out the free radical polymerization reaction on the pyrrole monomers to obtain the Fe3O4-SiO2-polypyrrole functional particles. The heavy-metal ion adsorbent has the characteristics of stable property, high adsorption rate and reproducibility, has favorable adsorption and recovery properties on dichromic ions, and has the advantages of simple preparation process, low material price and no environmental pollution. Compared with the normal chemical absorbent, the product of the invention is convenient to use and achieves the effects of waste water treatment, pollution abatement and environment protection.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

A method and apparatus for altering the charge distribution upon living membranes with functional stabilizational of the membrane physical electrical integrity further comprising a method for using submicroscopic, quadripolar, circular, center charged, energy balanced magnetic device in a four (4) magnet array of alternating polarity in which the magnetic poles are separated only by a distance which will allow a magnetic sphere of influence on all adjacent poles to suppress the firing of action potentials of mammalian sensory neurons. The method and apparatus further provides a static magnetic device for production of a magnetic field for treatment of various disorders. Further there is provided a static magnetic device for production of a magnetic field for treatment of disorders wherein the device provides a static magnetic pole of like polarity on the outer surface of the flux focusing ring adjacent to each of the 4 poles of the invention (focusing magnet) such that the end r top of the focusing magnet is oriented to the geometric side of the pole such that the a six of the two magnets form a 45 to 90 degree angle.

The invention provides a detection reagent for detecting hydroxy vitamin D, a preparation method of the detection reagent and application of the detection reagent to immunological detection of 25-hydroxy vitamin D. The detection reagent comprises a conjugate substance and a magnetic sphere, wherein the conjugate substance is formed by 25-hydroxy vitamin D antigen derivatives and protein carriers; the magnetic sphere is coated with the conjugate substance. By modifying the 25-hydroxy vitamin D antigen derivatives, the stability of the produced reagent is obviously improved, and the specificity and accuracy in the detection of the 25-hydroxy vitamin D are also improved. The invention further provides a kit for detecting 25-hydroxy vitamin D; the kit comprises the detection reagent, and the kit is good in stability, high in accuracy and high in universality.

The invention provides a synthetic method and application of a metal-organic framework (MOF) composite nanomaterial. The method comprises the following steps: dispersing ferriferrous oxide magnetic spheres which are synthesized through a traditional hydrothermal technology in a weakly alkaline solution of dopamine hydrochloride to carry out self-polymerization of dopamine on the surfaces of the magnetic spheres; and sequentially dispersing polydopamine coated magnetic spheres in a dimethylformamide solution of zirconium chloride and a dimethylformamide solution of 2-amino-terephthalic acid to obtain the MOF composite nanomaterial with the magnetic sphere surfaces coated with polydopamine and modified with an amino group and with zirconium as a center metal ion. The material has the advantages of large specific surface area, good hydrophilicity and suitable pore structure, can be applied to further researches of the proteomics, and can specifically enrich Which can specifically enrich phosphorylated peptide segments and glycopeptides; the synthetic method is simple and quick; and the synthesized material has good hydrophilicity and biocompatibility, and can be used for selectively enriching endogenous phosphorylation peptide segments and glycopeptide in complex biological samples.

The invention relates to a method and a detection kit used for detecting a virus. The method comprises steps that: an antibody of the glycoprotein of a target virus adventitia is coupled with magnetic nano / micro-spheres, such that immunized magnetic nano / micro-spheres are obtained; the immunized magnetic nano / micro-spheres are used for catching the target virus in the sample, such that a magnetic sphere virus composition is obtained; the antibody of the glycoprotein of the adventitia of the biotinylated virus is incubated with the magnetic sphere virus composition; the obtained material is incubated with streptavidin-coupled quantum dots, such that a magnetic sphere-virus-quantum dot composition is obtained; and the virus is detected through the detection upon fluorescence signals of the magnetic sphere-virus-quantum dot composition. With the method, specific recognition, preconcentration, separation, and high-sensitivity detection of H9N2 subtype avian influenza active virus can be realized. The invention also provides a detection kit employed by the method. The kit and the method provided by the invention are characterized by simple operation, high specificity, high sensitivity, and the like. The whole detection process can be finished in one hour.

The invention belongs to the field of modern isolation analysis materials science and engineering, it relates to preparation method and application of microwave assistant molecular print magnetic microsphere, the procedure as follows: adopting microwave chemical reactor, taking nanometer magnetic fluid as magnetic nuclei, by cleaning and external modification, ultrasound detracting to water; mounted pattern molecule and functional monomer self-assembly; adding polymeric liquor by step, cross link agent and initiator, making it mixes and separates with magnetic fluid fully by stirring of machine, microwave procedure controlled temperature and heats up, until raising polymerization; Magnetic separates the foregoing magnetic microsphere, removing mounted pattern molecule, vacuum drying, aging. The molecular print magnetic microsphere has not only recognize ability of mounted pattern molecule and its analogue, but also has the approved characteristic of 'gathering hyphen recycle', the process of preparation heats up with microwave, decreasing the time of print aggregation, improving homogeneity and print efficiency of polymer form. The molecular print magnetic sphere applies to gather, isolate and analysis of structure analogue in the environmental sample, eatable etc complex samples.

The present invention provides a method for preparing nanometer micron composite spheres, a nanometer particle having surface function group is linked to the specialised spheres surface by chemical method, so as to prepare stable nanometer micron composite spheres, and enhance the application and life of the composite spheres, these nanometer micron composite spheres are used for high-effect ion chromatography fillings, biomolecule separation and analyzing medium, cell separating magnetic spheres, catalyst and zymophore, diagnostic reagent, medical controlled released carrier etc.

The invention provides a chemiluminiscence detection kit for 17alpha-hydroxyprogesterone. The chemiluminiscence detection kit comprises a component A and a component B, wherein the component A is a magnetic sphere suspension coated by a 17alpha-hydroxyprogesterone antigen derivative, and the 17alpha-hydroxyprogesterone antigen derivative is coupled by a 17alpha-hydroxyprogesterone antigen and a protein carrier; the component B is a chemiluminiscence marker solution for marking a 17alpha-hydroxyprogesterone antibody. The chemiluminiscence detection kit has the advantage that according to the principle of competitive method, the content of 17alpha-hydroxyprogesterone in a sample can be accurately and sensitively detected.

An enrichment device for rare cells comprises a cell screening part and a cell collecting part. The cell screening part comprises two cell screening channels formed between the two opposite surfaces, at least one of the two opposite surfaces is a magnetic adsorption surface, the thickness of the cell screening channels is arranged in the mode that when cell suspension flows through the cell screening channels, a thin liquid layer enabling cells to be distributed in a single-layer mode basically is formed, and therefore when most cells combined with immunity magnetic spheres are adsorbed to the magnetic adsorption surface in the cell screening channels, the rare cells are prevented from being wrapped. The cell collecting part comprises a filtering unit, and the filtering unit is detachably arranged on the downstream portion of the cell screening part to intercept the rare cells which are not magnetically adsorbed. The two key problems of adsorption wrapping and centrifugal loss causing rare cell loss easily in the flotation enrichment process of the rare cells are solved, and therefore the recovery rate of the rare cells can be high. The invention further discloses a method for recovering the rare cells with the enrichment device.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU / mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU / mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

When the liquid is supplied to the inside of the cylinder from the lower side, the liquid stays in the cylinder, and the magnetic foreign matter is magnetically attracted to the magnetic ball. Only by supplying the cleaning liquid into the cylinder from the upper side, the magnetic foreign matter is discharged from the lower opening of the cylinder together with the cleaning liquid. At the transfer position, the magnetic foreign matter in the cleaning liquid is transferred from the cleaning liquid to the outer peripheral surface of the magnetic separator on the transfer side.

The invention relates to a chemiluminescence immunity detection reagent kit for detecting angiotensin I and a preparation method thereof. The reagent kit comprises a component A and a component B, wherein the component A is an angiotensin I antigen or an adapter of an angiotensin I antigen and a protein carrier, the component B is an anti-angiotensin I antibody, one of the component A and the component B is marked with a trace marker, and the other one is coated with magnetic spheres. The invention further relates to a method for detecting concentration of the angiotensin I by utilizing the reagent kit. According to the angiotensin I detection reagent kit, the concentration of the angiotensin I is determined by utilizing the reagent kit, so that the sensitivity and accuracy in detection are high.

The invention belongs to the technical field of microimaging, and discloses a magnetic tweezer device for controlling the motion of single molecule. The device comprises a sample pool, a magnet control unit, an imaging unit and a control unit, wherein the sample pool is used for containing a single molecule sample and solution; the single molecule sample is positioned in the solution; one end of the single molecule sample is connected to the bottom part of a piece of cover glass of the sample pool and while the other end of the single molecule sample is connected with a magnetic ball; the magnet control unit is positioned below the sample pool and used for controlling the motion of the magnetic ball; the imaging unit is used for transmitting light to the sample pool and outputting a reflected light signal that records the motion state of the magnetic ball; the control unit is used for receiving and converting the reflected light signal and measuring and analyzing the motion of the magnetic call. According to the magnetic tweezer device, a vertical up-down tweezer is provided, which avoids system error caused by gravity, and the measurement precision is also greatly raised.

The invention relates to an antibody-coupled bionic immune magnetic sphere and a preparation method of the antibody-coupled bionic immune magnetic sphere, belonging to the technical field of nanometer materials. According to the bionic immune magnetic sphere, the water-soluble Fe3O4 nanocluster is taken as the core, a cell membrane coats the core, and an antibody with a modification group is bonded outside the cell membrane through covalent coupling. The preparation method of the antibody-coupled bionic immune magnetic sphere comprises the following steps: preparing the Fe3O4 nanocluster, namely, MNC; preparing cell membrane fragments modified by choline azide, namely, M; reacting MCN and M to obtain a bionic magnetic sphere, and reacting the modification group with the antibody to obtain the antibody with the cyclo-ethynylation modification group; and coupling bionic magnetic sphere solution and the antibody with the cyclo-ethynylation modification group, thus obtaining the bionic immune magnetic sphere provided by the invention. The bionic immune magnetic sphere is good in dispersibility, rapid in magnetic response, and high in identifying efficiency when used for capturing tumor cells, no white blood cells exist in the background, and the captured tumor cells are good in activity. For the preparation method, the raw materials are easily available and have universality in the construction of the bionic immune magnetic sphere.

The invention belongs to the biologic field of micro algae, relating to a method for cleaning inner wall of pipeline type photobioreactor pipe in micro algae culture. Magnetic sphere is placed into a pipeline type photobioreactor, culture solution flows when being pushed by power, the magnetic sphere randomly enters into the pipeline of photobioreactor along with the culture solution and does directional movement or slides under manual regulation of a control system outside the pipeline, and the magnetic sphere continuously slightly collides and rubs pipe wall in circulated moving process in the photobioreactor pipeline, so that algae cell material adhered onto transparent pipeline inner wall is rubbed into liquid phase used for culturing micro algae, the pipeline photobioreactor pipeline is effectively cleaned and light transmission thereof is maintained. The invention can effectively clean cells into algae liquid phase at initial stage of adherence, or substance such as cell adhered to the inner wall of the pipeline type photobioreactor pipe is removed, restriction of micro algae cell adherence to micro algae cell culture is overcome, and good light transmission and culture effect of photobioreactor are maintained.

A permanent magnet comprises a shell surrounding a cavity. The shell has a magnetic remanence Br(θ) configured such that a magnetic field taper extends through the cavity and wherein the shell includes a non-distortive access region that is substantially magnetic field.

The invention discloses a carbohydrate antigen CA125 quantitative determination kit. The kit comprises a calibration substance, a quality control substance, a resisting reagent, a magnetic particle reagent and a luminescence substrate. The invention also discloses a making method of the kit, and a method for detecting a carbohydrate antigen CA125 by using the kit. The resisting reagent is prepared by adopting a fluorescein isothiocyanate labeled CA125 coated antibody and an alkaline phosphatase labeled CA125 labeled antibody, and the magnetic particle reagent is prepared through coupling an anti-fluorescein isothiocyanate antibody with a carboxyl magnetic sphere, so uniform mixing and separation are easy in an immunization reaction, and the reaction speed is greatly improved; and a novel chemiluminescence substrate APLS is adopted as the substrate, so the sensitivity and the specificity of the kit are improved. The determination kit has the advantages of reliable performances, high sensitivity and wide linear range, and can be used together with a semi-automatic and fully-automatic apparatus.

The invention discloses a ferrocene-methylene blue double-labelled magnetic sphere nano-composite for detecting miRNA and a preparation method of the magnetic sphere nano-composite. The magnetic sphere nano-composite contains the following components: a magnetic sphere nanogold composite, hairpin DNA probes, ferrocene modified nanogold, methylene blue modified nanogold and miRNA, wherein miRNA is complementary with annular parts of the hairpin DNA probes. The preparation method comprises the steps of firstly preparing the magnetic sphere nanogold composite, fixing the two hairpin DNA probes to the surface of the magnetic sphere nanogold composite, carrying out miRNA hybridization, and fixing the ferrocene modified nanogold and the methylene blue modified nanogold. The magnetic sphere nano-composite provided by the invention has the advantages of simplicity in preparation, strong electrochemical responsiveness, high detection sensitivity and the like.

The invention provides an aptamer nucleic acid probe kit for detecting doxycycline residue as well as a preparation method and application thereof. A nanometer magnetic sphere is an inner core and the outer surface of the nanometer magnetic sphere is bonded with a doxycycline nucleic acid aptamer chain, wherein the doxycycline nucleic acid aptamer chain is 5'-GCATGCCTTAAGCGATCG-N35-CCATATTATAAGGCATGC-3', N represents any one of A, T, C and G, N35 represents that random fragment length comprises 35 basic groups, and the probe is suitable for detection of doxycycline residue in animal body.

The invention discloses a method for enriching leucomalachite green and leucogentian violet in aquatic products, and relates to a new method for preprocessing a sample in the field of the safe analysis of foods. The method comprises the following steps: preprocessing a sample, adding super-paramagnetic nanometer or submicron magnetic spheres with the surfaces modified with a C18 alkyl chain for adsorption, eluting, and collecting the obtained eluate; and directly adding the eluate to a liquid chromatograph or a liquid chromatograph-mass spectrometer, and quantitatively analyzing the contents of leucomalachite green and leucogentian violet. The method has the characteristics of simple operation flow, high extraction and recovery rates, less consumption of elution solvents, and no need of rotary evaporation.

Provided are a detection agent for detecting hydroxy vitamin D, preparation method thereof, and use thereof in 25-hydroxy vitamin D immunological detection. The detection agent comprises a conjugate formed by a 25-hydroxy vitamin D antigen derivative and protein carrier, and magnetic spheres coated by the conjugate. Also provided is a 25-hydroxy vitamin D detection kit comprising the detection agent.

The invention relates to a chemiluminesent immunoassay kit for detecting angiotensin II and a preparation method of the chemiluminesent immunoassay kit. The kit comprises a component A and a component B, wherein the component A is angiotensin II antigen or a junctional complex of the angiotensin II antigen and a protein carrier; the component B is an angiotensin II antibody; one of the component A and the component B is used for marking a tracer marker; and the other one is enveloped by magnetic spheres. The invention further relates to a method for detecting the concentration of the angiotensin II by virtue of the kit. According to the chemiluminesent immunoassay kit, the concentration of the angiotensin II is measured by the kit provided by the invention; and high detection sensitivity and accuracy can be obtained.

The invention discloses a modified lectin wrapped magnetic macromolecular liposome nano microsphere, a preparation method and an application. The lectin wrapped magnetic macromolecular liposome nano microsphere is macromolecular liposome with a double membrane structure, which is formed by wrapping a magnetic nano particle with modified lectin and lipid after forming a lipid membrane. Different from the traditional magnetic nano microsphere surface grafting modification, the magnetic liposome directly employs a derivative of the active substance lectin, lentil lectin-hexadecyl quaternary ammonium salt to wrap the magnetic nano particle, and the method can control a lectin content of the surface of the magnetic sphere and increase the content of the active substance such as the lectin. The lectin wrapped magnetic macromolecular liposome is applicable to the fields of magnetic resonance imaging (MRI), magnetic marking, magnetic rapid diagnosis, controlled medicine release, genetic vectors, magnetic high-temperature therapy, immunomagnetic microspheres and the like.

The invention discloses a fixing and protection device for a target sphere of a three-dimensional coordinate tracker. The fixing and protection device comprises a magnetic sphere base, wherein a magnetic groove is formed in the magnetic sphere base, the groove is used for fixing an objective target sphere, a plurality of horizontal threaded through holes are formed in the periphery of the magnetic sphere base, a bolt is arranged in each threaded through hole, one end of each threaded through hole is positioned in the groove, the lower part of the magnetic sphere base is connected with a vertical rotating shaft, the lower end of the rotating shaft is connected with a rotating block in a movable manner, the rotating shaft rotates around its axis, a horizontally-arranged fixed rod is sleeved in the rotating block, and the rotating block rotates around the axis of the fixed rod; and the lower parts of the fixing rod are connected with fixed bases. The fixing and protection device can guarantee the use of the magnetic sphere base, can further adjust angular positions of the objective target sphere so as to meet different requirements, and improves the measurement efficiency.

The invention relates to a preparation method for a monodisperse high-crosslinking-degree core-shell P(GMA-DVB) (glycidyl methacrylate-divinyl benzene) / Fe3O4 sphere, which is used for solving the technical problem that the monodisperse ferrate magnetic nanometer sphere prepared with the traditional preparation method is uniform in participle size. The invention provides a technical scheme as follows: the monodisperse nanoscale high-crosslinking-degree core-shell P(GMA-DVB) / Fe3O4 sphere with controllable particle size, high magnetic responsiveness and high magnetic content is prepared with a precipitation polymerization method in which monodisperse high-magnetic-responsiveness Fe3O4 prepared with a thermal solvent method is used as a kernel and the GMA is used as a functional monomer, and the prepared magnetic P(GMA-DVB) / Fe3O4 sphere has a particle size of 480-650 nanometers. Compared with the preparation method in the prior art, the preparation method disclosed by the invention has the advantages of controllable particle size of the magnetic sphere and greatly improved monodispersity and magnetic responsiveness of the prepared magnetic sphere. Meanwhile, a great deal of freedom and flexibility is brought to users for further functionalizing a polymer because a GMA monomer has a reactive epoxy group.

The invention relates to a single base mutation detection method combining nucleic acid temporary hybridizing and magnetic separating. The method comprises the following steps: A, designing a capturing probe and a signal probe according to a target gene; B, preparing a magnetic sphere solution from a magnetic sphere coupled with the capturing probe, adding the signal probe to conduct nucleic acid hybridization with a to-be-detected sample; C, conducting magnetic separation after finishing the nucleic acid hybridization, and then re-suspending the magnetic sphere in a low-metal ion solution; D, conducting magnetic separation again, detecting an obtained liquid supernatant, judging whether single base mutation exists in the to-be-detected sample, wherein the capturing probe is completely complementary with a non-mutation part of the target gene; the signal probe is completely complementary with a mutant type gene of the target gene, and forms a single base mismatch with a wild type gene of the target gene. The detection method is fast, and can be used in low-abundance mutation detection.

The invention belongs to the technical field of a precious metal catalyst, and discloses a polycarboxyl magnetic silicon nanosphere immobilized platinum catalyst as well as a preparation method and application thereof, wherein the catalyst is that platinum nanoparticles are uniformly dispersed on the surface of a polycarboxyl magnetic silicon nanosphere carrier; the preparation method adopts a nano ferrosoferric oxide magnetic sphere as a core; the nano ferrosoferric oxide magnetic silicon sphere is prepared by coating silicon dioxide by a sol-gel method, and carboxyl functionalization is performed to obtain a polycarboxyl magnetic silicon nanosphere carrier, and then the metal platinum is immobilized to obtain the polycarboxyl magnetic silicon nanosphere immobilized platinum catalyst. A cheap and easily available material is adopted as an immobilizing material, phosphorus or sulfur is not involved in the preparation process, and the polycarboxyl magnetic silicon nanosphere immobilizedplatinum catalyst is prepared which has high activity, selectivity and reusability and is successfully applied to hydrosilylation between n-hexene, n-heptylene, n-octylene, trans-2-hexene, cis-2-hexene, trans-3-hexene, cis-3-hexene and dichloromethylsilane.