157 results about "Magnetic sphere" patented technology

The invention relates to a synthetic method for a magnetic metal organic framework composite material coated by [Cu2(btc)2] on surfaces of ferroferric oxide microspheres and application of the composite material. The method comprises the following steps of: firstly synthesizing ferroferric oxide microspheres by a hydrothermal synthesis method; dispersing magnetic spheres in an ethanol liquid of mercaptoacetic acid, wherein hydroxyls are formed on the surface of the spheres; dispersing mercaptoacetic acid modified magnetic spheres to an ethanol liquid of copper acetate, reacting for 15 minutes at 70 DEG C, and then dispersing the product in an ethanol liquid of trimesic acid and reacting for 30 minutes at 70 DEG C; and performing alternate reaction of magnetic spheres with copper acetate and the ethanol liquid of trimesic acid to finally, obtain the magnetic metal organic framework composite material with a core-shell structure. The material has a metal organic framework shell layer and can be coordinated with peptide fragments with amino groups and carboxylic group so as to enrich low concentration peptide. Meanwhile, the enriching and separating process is fast, simple and convenient due to high paramagnetism of ferroferric oxide. The synthetic method is simple and low in cost, and can be used for enrichment and separation of low abundance peptide fragments less than 1nM and MALDI-TOFMS (Matrix-Assisted Laser Desorption Ionization-Time Of Flight Mass Spectrometer) detection.

The invention relates to a preparation method of a protein magnetic imprinting nano sphere, in particular to a preparation method of a protein magnetic imprinting nano sphere which takes one of hemoglobin, muramidase or serum protein as template molecules; the preparation method includes the following steps: synthesizing magnetic nano particles which are Fe3O4 nano particles covered with silicon dioxide on the surfaces; carrying out silanization to the amino-groups on the surface of the magnetic sphere; using glutaric dialdehyde to connect protein; using silylation agent to fix the space structure of template protein; alkaline eluting the protein on the surface of the magnetic sphere to form imprinting sites. The hemoglobin, muramidase or serum protein magnetic nano imprinting polymer sphere prepared by the invention combines the precise and specific identification property of molecular imprinting and the quick separation property of the magnetic nano sphere under the action of an external magnetic field, integrates the advantages of molecular imprinting and the magnetic nano sphere, avoids complex centrifugation operation, plays vital roles in the separation of cells, protein and nucleic acid, the detection of biomolecules, tumor diagnosis and medicine targeting therapy and has promising application prospect in the field of isolation analysis.

The invention provides a synthetic method and application of a metal-organic framework (MOF) composite nanomaterial. The method comprises the following steps: dispersing ferriferrous oxide magnetic spheres which are synthesized through a traditional hydrothermal technology in a weakly alkaline solution of dopamine hydrochloride to carry out self-polymerization of dopamine on the surfaces of the magnetic spheres; and sequentially dispersing polydopamine coated magnetic spheres in a dimethylformamide solution of zirconium chloride and a dimethylformamide solution of 2-amino-terephthalic acid to obtain the MOF composite nanomaterial with the magnetic sphere surfaces coated with polydopamine and modified with an amino group and with zirconium as a center metal ion. The material has the advantages of large specific surface area, good hydrophilicity and suitable pore structure, can be applied to further researches of the proteomics, and can specifically enrich Which can specifically enrich phosphorylated peptide segments and glycopeptides; the synthetic method is simple and quick; and the synthesized material has good hydrophilicity and biocompatibility, and can be used for selectively enriching endogenous phosphorylation peptide segments and glycopeptide in complex biological samples.

The invention relates to a method and a detection kit used for detecting a virus. The method comprises steps that: an antibody of the glycoprotein of a target virus adventitia is coupled with magnetic nano/micro-spheres, such that immunized magnetic nano/micro-spheres are obtained; the immunized magnetic nano/micro-spheres are used for catching the target virus in the sample, such that a magnetic sphere virus composition is obtained; the antibody of the glycoprotein of the adventitia of the biotinylated virus is incubated with the magnetic sphere virus composition; the obtained material is incubated with streptavidin-coupled quantum dots, such that a magnetic sphere-virus-quantum dot composition is obtained; and the virus is detected through the detection upon fluorescence signals of the magnetic sphere-virus-quantum dot composition. With the method, specific recognition, preconcentration, separation, and high-sensitivity detection of H9N2 subtype avian influenza active virus can be realized. The invention also provides a detection kit employed by the method. The kit and the method provided by the invention are characterized by simple operation, high specificity, high sensitivity, and the like. The whole detection process can be finished in one hour.

The invention belongs to the field of modern isolation analysis materials science and engineering, it relates to preparation method and application of microwave assistant molecular print magnetic microsphere, the procedure as follows: adopting microwave chemical reactor, taking nanometer magnetic fluid as magnetic nuclei, by cleaning and external modification, ultrasound detracting to water; mounted pattern molecule and functional monomer self-assembly; adding polymeric liquor by step, cross link agent and initiator, making it mixes and separates with magnetic fluid fully by stirring of machine, microwave procedure controlled temperature and heats up, until raising polymerization; Magnetic separates the foregoing magnetic microsphere, removing mounted pattern molecule, vacuum drying, aging. The molecular print magnetic microsphere has not only recognize ability of mounted pattern molecule and its analogue, but also has the approved characteristic of 'gathering hyphen recycle', the process of preparation heats up with microwave, decreasing the time of print aggregation, improving homogeneity and print efficiency of polymer form. The molecular print magnetic sphere applies to gather, isolate and analysis of structure analogue in the environmental sample, eatable etc complex samples.

The present invention provides a method for preparing nanometer micron composite spheres, a nanometer particle having surface function group is linked to the specialised spheres surface by chemical method, so as to prepare stable nanometer micron composite spheres, and enhance the application and life of the composite spheres, these nanometer micron composite spheres are used for high-effect ion chromatography fillings, biomolecule separation and analyzing medium, cell separating magnetic spheres, catalyst and zymophore, diagnostic reagent, medical controlled released carrier etc.

The invention relates to a method for detecting bacteria. The method comprises the steps as follows: coupling magnetic nanospheres and fluorescent nanospheres with mouse typhus salmonella antibodies so as to obtain mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres, and adding the mouse typhus salmonella-targeted immunologic magnetic spheres and immunologic fluorescent spheres into a detection system so as to realize magnetic capture and fluorescent labeling to the mouse typhus salmonella simultaneously. About 10 CFU/mL of the mouse typhus salmonella can be detected through observation of a fluorescent microscope; and a good quantitative relation is obtained through detection of a fluorescence spectrophotometer, the linear range is 105-107 CFU/mL, and R2 is equal to 0.9994. The whole detection process is very simple, high in sensitivity and strong in specificity, and can be finished detection within 1.5 h.

The invention relates to a chemiluminescence immunity detection reagent kit for detecting angiotensin I and a preparation method thereof. The reagent kit comprises a component A and a component B, wherein the component A is an angiotensin I antigen or an adapter of an angiotensin I antigen and a protein carrier, the component B is an anti-angiotensin I antibody, one of the component A and the component B is marked with a trace marker, and the other one is coated with magnetic spheres. The invention further relates to a method for detecting concentration of the angiotensin I by utilizing the reagent kit. According to the angiotensin I detection reagent kit, the concentration of the angiotensin I is determined by utilizing the reagent kit, so that the sensitivity and accuracy in detection are high.

The invention relates to an antibody-coupled bionic immune magnetic sphere and a preparation method of the antibody-coupled bionic immune magnetic sphere, belonging to the technical field of nanometer materials. According to the bionic immune magnetic sphere, the water-soluble Fe3O4 nanocluster is taken as the core, a cell membrane coats the core, and an antibody with a modification group is bonded outside the cell membrane through covalent coupling. The preparation method of the antibody-coupled bionic immune magnetic sphere comprises the following steps: preparing the Fe3O4 nanocluster, namely, MNC; preparing cell membrane fragments modified by choline azide, namely, M; reacting MCN and M to obtain a bionic magnetic sphere, and reacting the modification group with the antibody to obtain the antibody with the cyclo-ethynylation modification group; and coupling bionic magnetic sphere solution and the antibody with the cyclo-ethynylation modification group, thus obtaining the bionic immune magnetic sphere provided by the invention. The bionic immune magnetic sphere is good in dispersibility, rapid in magnetic response, and high in identifying efficiency when used for capturing tumor cells, no white blood cells exist in the background, and the captured tumor cells are good in activity. For the preparation method, the raw materials are easily available and have universality in the construction of the bionic immune magnetic sphere.

A permanent magnet comprises a shell surrounding a cavity. The shell has a magnetic remanence B_r(θ) configured such that a magnetic field taper extends through the cavity and wherein the shell includes a non-distortive access region that is substantially magnetic field.

The invention discloses a ferrocene-methylene blue double-labelled magnetic sphere nano-composite for detecting miRNA and a preparation method of the magnetic sphere nano-composite. The magnetic sphere nano-composite contains the following components: a magnetic sphere nanogold composite, hairpin DNA probes, ferrocene modified nanogold, methylene blue modified nanogold and miRNA, wherein miRNA is complementary with annular parts of the hairpin DNA probes. The preparation method comprises the steps of firstly preparing the magnetic sphere nanogold composite, fixing the two hairpin DNA probes to the surface of the magnetic sphere nanogold composite, carrying out miRNA hybridization, and fixing the ferrocene modified nanogold and the methylene blue modified nanogold. The magnetic sphere nano-composite provided by the invention has the advantages of simplicity in preparation, strong electrochemical responsiveness, high detection sensitivity and the like.

The invention provides an aptamer nucleic acid probe kit for detecting doxycycline residue as well as a preparation method and application thereof. A nanometer magnetic sphere is an inner core and the outer surface of the nanometer magnetic sphere is bonded with a doxycycline nucleic acid aptamer chain, wherein the doxycycline nucleic acid aptamer chain is 5'-GCATGCCTTAAGCGATCG-N35-CCATATTATAAGGCATGC-3', N represents any one of A, T, C and G, N35 represents that random fragment length comprises 35 basic groups, and the probe is suitable for detection of doxycycline residue in animal body.

The invention discloses a method for enriching leucomalachite green and leucogentian violet in aquatic products, and relates to a new method for preprocessing a sample in the field of the safe analysis of foods. The method comprises the following steps: preprocessing a sample, adding super-paramagnetic nanometer or submicron magnetic spheres with the surfaces modified with a C18 alkyl chain for adsorption, eluting, and collecting the obtained eluate; and directly adding the eluate to a liquid chromatograph or a liquid chromatograph-mass spectrometer, and quantitatively analyzing the contents of leucomalachite green and leucogentian violet. The method has the characteristics of simple operation flow, high extraction and recovery rates, less consumption of elution solvents, and no need of rotary evaporation.

The invention relates to a chemiluminesent immunoassay kit for detecting angiotensin II and a preparation method of the chemiluminesent immunoassay kit. The kit comprises a component A and a component B, wherein the component A is angiotensin II antigen or a junctional complex of the angiotensin II antigen and a protein carrier; the component B is an angiotensin II antibody; one of the component A and the component B is used for marking a tracer marker; and the other one is enveloped by magnetic spheres. The invention further relates to a method for detecting the concentration of the angiotensin II by virtue of the kit. According to the chemiluminesent immunoassay kit, the concentration of the angiotensin II is measured by the kit provided by the invention; and high detection sensitivity and accuracy can be obtained.

The invention discloses a modified lectin wrapped magnetic macromolecular liposome nano microsphere, a preparation method and an application. The lectin wrapped magnetic macromolecular liposome nano microsphere is macromolecular liposome with a double membrane structure, which is formed by wrapping a magnetic nano particle with modified lectin and lipid after forming a lipid membrane. Different from the traditional magnetic nano microsphere surface grafting modification, the magnetic liposome directly employs a derivative of the active substance lectin, lentil lectin-hexadecyl quaternary ammonium salt to wrap the magnetic nano particle, and the method can control a lectin content of the surface of the magnetic sphere and increase the content of the active substance such as the lectin. The lectin wrapped magnetic macromolecular liposome is applicable to the fields of magnetic resonance imaging (MRI), magnetic marking, magnetic rapid diagnosis, controlled medicine release, genetic vectors, magnetic high-temperature therapy, immunomagnetic microspheres and the like.

The invention discloses a fixing and protection device for a target sphere of a three-dimensional coordinate tracker. The fixing and protection device comprises a magnetic sphere base, wherein a magnetic groove is formed in the magnetic sphere base, the groove is used for fixing an objective target sphere, a plurality of horizontal threaded through holes are formed in the periphery of the magnetic sphere base, a bolt is arranged in each threaded through hole, one end of each threaded through hole is positioned in the groove, the lower part of the magnetic sphere base is connected with a vertical rotating shaft, the lower end of the rotating shaft is connected with a rotating block in a movable manner, the rotating shaft rotates around its axis, a horizontally-arranged fixed rod is sleeved in the rotating block, and the rotating block rotates around the axis of the fixed rod; and the lower parts of the fixing rod are connected with fixed bases. The fixing and protection device can guarantee the use of the magnetic sphere base, can further adjust angular positions of the objective target sphere so as to meet different requirements, and improves the measurement efficiency.

The invention relates to a single base mutation detection method combining nucleic acid temporary hybridizing and magnetic separating. The method comprises the following steps: A, designing a capturing probe and a signal probe according to a target gene; B, preparing a magnetic sphere solution from a magnetic sphere coupled with the capturing probe, adding the signal probe to conduct nucleic acid hybridization with a to-be-detected sample; C, conducting magnetic separation after finishing the nucleic acid hybridization, and then re-suspending the magnetic sphere in a low-metal ion solution; D, conducting magnetic separation again, detecting an obtained liquid supernatant, judging whether single base mutation exists in the to-be-detected sample, wherein the capturing probe is completely complementary with a non-mutation part of the target gene; the signal probe is completely complementary with a mutant type gene of the target gene, and forms a single base mismatch with a wild type gene of the target gene. The detection method is fast, and can be used in low-abundance mutation detection.

The invention belongs to the technical field of a precious metal catalyst, and discloses a polycarboxyl magnetic silicon nanosphere immobilized platinum catalyst as well as a preparation method and application thereof, wherein the catalyst is that platinum nanoparticles are uniformly dispersed on the surface of a polycarboxyl magnetic silicon nanosphere carrier; the preparation method adopts a nano ferrosoferric oxide magnetic sphere as a core; the nano ferrosoferric oxide magnetic silicon sphere is prepared by coating silicon dioxide by a sol-gel method, and carboxyl functionalization is performed to obtain a polycarboxyl magnetic silicon nanosphere carrier, and then the metal platinum is immobilized to obtain the polycarboxyl magnetic silicon nanosphere immobilized platinum catalyst. A cheap and easily available material is adopted as an immobilizing material, phosphorus or sulfur is not involved in the preparation process, and the polycarboxyl magnetic silicon nanosphere immobilizedplatinum catalyst is prepared which has high activity, selectivity and reusability and is successfully applied to hydrosilylation between n-hexene, n-heptylene, n-octylene, trans-2-hexene, cis-2-hexene, trans-3-hexene, cis-3-hexene and dichloromethylsilane.