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582 results about "Quantitative determination" patented technology

Quantitative analysis refers to the determination of how much of a given component is present in a sample. The quantity may be expressed in terms of mass, concentration, or relative abundance of one or all components of a sample.

Fatigue performance analysis method for asphalt mixture of asphalt pavement

The invention belongs to the field of asphalt mixture performance analysis, and particularly discloses a fatigue performance analysis method for an asphalt mixture of an asphalt pavement, which comprises the following steps of: synchronously acquiring a dynamic strain field and a temperature field in a material by fusing an embedded sensor in a paving and rolling construction process; a temperature-strain coupled fatigue damage evolution model is combined to construct an on-line fatigue performance analysis system oriented to the whole construction process, so that a complex heat-force coupling environment and a non-uniform compaction process in actual paving operation are effectively restored; scale effect deviation and boundary condition distortion caused by difference between laboratory test piece preparation and on-site working conditions are remarkably reduced, and meanwhile, in the online detection process of the fatigue performance of the asphalt mixture, instant quantitative judgment of the performance state of the pavement structure is realized by positioning a damage remarkable overrun area; and the in-situ monitoring capability on the initial fatigue damage is fully reflected, so that the abnormal strain accumulation process before microcrack initiation can be captured.
Owner:ANHUI CONSTR ENG CHANGFENG CONSTR ENG CO LTD +1

Method for detecting impurities in moxifloxacin hydrochloride bulk drug

The invention relates to the technical field of drug analysis and detection, and provides a detection method of moxifloxacin hydrochloride related substances. The related substances of moxifloxacin hydrochloride are detected by adopting a high performance liquid chromatography method, and the detection conditions are as follows: octadecylsilane chemically bonded silica is taken as a filling agent of a chromatographic column; the wavelength of a UV detector is 296 nm; the column temperature is 35-45 DEG C; the flow velocity of the mobile phase is 1.6-1.8 ml / min; the sample injection volume is 10-50 [mu] l; the mobile phase A is phosphate buffer solution-acetonitrile-methanol; the mobile phase B is phosphate buffer solution-acetonitrile-methanol; the elution mode is gradient elution; the diluent is a mobile phase A; according to the method provided by the invention, effective separation between moxifloxacin hydrochloride and various impurities can be realized, the sensitivity is high, the accuracy is good, the reproducibility is relatively good, a good linear relation is shown in a certain range, and qualitative and quantitative determination can be carried out on various impurities in moxifloxacin hydrochloride bulk drugs.
Owner:NANJING CHIA TAI TIANQING PHARMA

Cutter head for shield machine and shield construction method

PCT designated stage expiredWO2025025524A1TunnelsMicrowaveQuantitative determination
A cutter head for a shield machine and a shield construction method. In the shield construction method, force sensors measure the pressure borne by hobs (103), so as to determine, on the basis of pressure signals, the hardness of rock being processed currently, when the pressure signals reach or exceed a preset value, a controller is started to control microwave rock breaking systems (104) to heat the rock being processed currently, and the specific mode is to provide, on the basis of the pressure signals and a reference signal, a quantitative determination mode for whether the microwave rock breaking systems (104) need to be started, such that the control is more convenient, and energy is saved. Furthermore, the physical characteristics of the rock are changed, so that cracks appear in the rock, thereby reducing the rock strength, and reducing the abrasion of a shield machine cutter. By prolonging the service life of cutters and shortening the shutdown maintenance and cutter replacement time, the construction efficiency is improved, and the construction duration is shortened.
Owner:CHINA RAILWAY 14TH BUREAU GROUP EQUIPMENT CO LTD

LC-ms / ms quantitative determination method for Anti-novel coronavirus bispecific antibody drug

PCT designated stage expiredWO2024251258A1Component separationViral antibodySolid phase extraction
The present application relates to the technical field of antibody drug determination, and provides a liquid chromatography-tandem mass spectrometry (LC-MS / MS) quantitative determination method for an anti-novel coronavirus bispecific antibody drug. According to the method, characteristic peptide segments EAYGMDVWGQGTTVTVSSASTK and SLSLSPGGGSQVQLVQSGAEVK are used, and on the basis that the characteristic peptide segments comprise a stable isotope internal standard, the content of the anti-novel coronavirus bispecific antibody drug is accurately quantified on an LC-MS / MS platform by means of the process of protein precipitation, enzymolysis and solid-phase extraction. According to the present application, the method and the characteristic peptide segments are highly selective and specific for anti-novel coronavirus bispecific antibody YBSW015, without being interfered by a pre-existing anti-novel coronavirus antibody in a patient, have the characteristics of high efficiency and simplicity, and can be used for clinical monitoring of the anti-novel coronavirus bispecific antibody.
Owner:SUZHOU YABAO PHARMA R&D CO LTD

Colorimetric-Raman coding immunochromatography test strip based on 3D film-shaped SERS probe and multi-bacterium detection method of colorimetric-Raman coding immunochromatography test strip

The invention relates to the technical field of bacterium detection, in particular to a colorimetric-Raman coding immunochromatography test strip based on a 3D film-shaped SERS probe and a multi-bacterium detection method thereof. According to the technical scheme, the kit comprises a sample pad, a nitrocellulose membrane, a water absorption pad, a 3D membrane-like SERS probe and an operation buffer solution. According to the present invention, the immunochromatography of the 3D film-like probe is provided, the MoDAu-coated Ag SERS nano-film is adopted as the probe in the system, such that the pathogenic bacteria can be highly specifically recognized and captured, the immune complex fluidity can be increased, the rapid quantitative detection of the three pathogenic bacteria can be achieved, and the flexible 3D film-like SERS probe capable of enhancing the colorimetric ability and the SERS activity and highly specifically capturing the pathogenic bacteria can be prepared; by combining with an antibody-modified ICA test strip, rapid, simultaneous and quantitative determination of various pathogenic bacteria is realized.
Owner:THE FIRST AFFILIATED HOSPITAL OF ANHUI MEDICAL UNIV

Cosmetic production line safety monitoring method and system based on data analysis

The invention relates to the technical field of production safety and intelligent monitoring, in particular to a cosmetic production line safety monitoring method and system based on data analysis, and the method comprises the steps: obtaining real-time operation parameters through a multi-source data collection module, and generating a production environment dynamic mapping graph; extracting a fluctuation trend and abnormal distribution characteristics, and generating a region data adjustment instruction set; adjusting a signal acquisition strategy, and constructing a regional signal alignment state table; identifying the signal intensity variation, and forming a regional signal adjustment mark graph; and finally outputting a monitoring area state evaluation value list. According to the method, the signal fluctuation expression precision and the dynamic regulation and control capability can be remarkably improved, the adaptability and the response sensitivity of safety monitoring in a complex production environment are enhanced, and high-precision local compensation and equipment state quantitative judgment are realized.
Owner:有生之颜(深圳)生物科技有限公司

Analysis method and system based on high impact meteorological-power event modeling data

The invention discloses an analysis method and system based on high impact meteorological-power event modeling data, and belongs to the technical field of electric digital data processing. The method comprises the following steps: performing quantitative judgment on the fan state according to fan state parameters of each fan in a wind power plant to obtain a fan state quantitative judgment result, judging whether to perform fan dynamic processing or not based on the fan state quantitative judgment result, and obtaining each normal fan according to the fan dynamic processing result; performing quantitative judgment on wind speed fluctuation according to the wind speed fluctuation parameter of each normal fan in each monitoring time period in the wind power plant to obtain a wind speed fluctuation quantitative judgment result, and judging whether to perform power generation capacity dynamic correction and ensemble empirical mode decomposition method optimization or not based on the wind speed fluctuation quantitative judgment result; the optimized ensemble empirical mode decomposition method is used for meteorological-power data analysis, and the accuracy of high-impact meteorological-power event modeling data analysis is improved.
Owner:STATE QIHOU CENT +1

Bedrock fracture intrinsic parameter quantitative determination method and system based on all-angle domain amplitude and phase joint inversion

The invention belongs to the technical field of geophysical exploration, and particularly relates to a method and a system for quantitatively measuring intrinsic parameters of bedrock fractures based on all-angle domain amplitude and phase joint inversion. The method comprises the following steps: acquiring geological radar reflection data of a target fracture at a plurality of incident angles; synchronously extracting data sequences of amplitude change along with angles and phase change along with angles; establishing a fracture reflection theoretical model; constructing a target function jointly constrained by the amplitude residual and the phase residual; and adopting a global optimization algorithm to minimize the target function, and synchronously inverting to obtain the fracture opening, the filler dielectric constant and the occurrence parameter. According to the invention, through the combined constraint of the amplitude and the phase, the inversion multiplicity is effectively inhibited; accurate system effect correction is adopted, so that the reliability of large-angle data is ensured; according to the method, multi-parameter synchronous inversion without prior hypothesis is realized, the inversion precision is small in relative error, the method is obviously superior to a traditional method, and the method has important engineering application value.
Owner:YELLOW RIVER CONSERVANCY TECHN INST

Microfluidic reactor for electrocatalytic determination of chemical oxygen demand

The invention relates to a microfluidic reactor for electrocatalytic determination of chemical oxygen demand, and belongs to the field of environmental monitoring. In the reactor, to-be-detected samples are injected from a sample inlet positioned at the front end of a liquid inlet and flow into the reactor formed by stacking three layers of components from the liquid inlet below along with electrolyte, and the to-be-detected samples positioned at different horizontal heights turn along with liquid flow and finally reach the surface of a planar anode at the bottom under the action of a pinch plate distributed with a micro cone group; and the water flows upwards through a hole in one end of the top side of the buckle plate, passes through the auxiliary electrode with the hole and finally flows out of the electrolytic tank through a water outlet in the top. Organic matters in the to-be-detected sample are subjected to electrochemical oxidation reaction under the action of the catalyst on the surface of the planar anode at the bottom of the reactor, and a generated electric signal is used for quantitatively determining the chemical oxygen demand of the to-be-detected water sample. In the invention, the pinch plate distributed with the micro cone group can strengthen organic matters to break through stable horizontal laminar mass transfer, so that the organic matters in the sample to be detected fully reach the surface of the plane anode to generate electrochemical oxidation reaction, thereby improving electric signals and lower limit of detection. According to the characteristics, a more stable and accurate electric signal can be obtained when the reactor is used for measuring the chemical oxygen demand, and the linear range of the measurement is widened, so that the efficient and accurate measurement of the chemical oxygen demand in a sample to be measured is realized.
Owner:INST OF URBAN ENVIRONMENT CHINESE ACAD OF SCI +1

Quality evaluation system and method for giant salamander by-product source functional factors

The invention relates to the technical field of giant salamander by-product quality evaluation, and discloses a giant salamander by-product source functional factor quality evaluation system and method, and the method comprises the steps: obtaining a giant salamander by-product sample, carrying out the pretreatment, and extracting a functional factor to obtain a to-be-detected sample; identifying and quantitatively determining specific markers based on biochemical analysis and molecular detection means to generate a marker data set; inputting the marker data into a multi-modal feature fusion module, extracting activity and stability features, and performing time sequence dynamic modeling; activity and stability independent evaluation branches are set, and corresponding score values are generated; dividing functional domains according to functional factor categories, constructing an evaluation curve based on historical data, substituting a target sample into each functional domain and an adjacent functional domain to generate functional domain evaluation results, and performing weighted fusion; and finally, carrying out weighted integration on the activity score, the stability score and the comprehensive evaluation value, and determining a final quality evaluation result.
Owner:CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY

Method for detecting activity of insect dsRNA degrading enzyme based on dual luciferase report system

The invention discloses a method for detecting insect dsRNA degrading enzyme activity based on a dual luciferase report system, and relates to the technical field of biological medicine. The method comprises the following steps: constructing an insect dsRNA degrading enzyme into a pAc5.1 / V5-His B plasmid to form a recombinant plasmid, co-transfecting the recombinant plasmid and a psiCHECKTM-2 plasmid into an insect cell for culturing, then respectively adding dsLuc and dsGFP into the cell, continuing culturing, finally collecting the cell, and detecting the enzyme activity of the cell. The system is constructed based on a dual-luciferase report system, and qualitative and quantitative enzyme activity is realized through three steps of cell culture, transfection and detection. Compared with in-vitro heterologous protein expression, agarose detection after in-vitro degradation reaction or RNA extraction and then fluorescent quantitative PCR (RT-qPCR) detection, the method provided by the invention is faster and more accurate, and is not limited to the first step in which the protein is difficult to obtain.
Owner:CHANGZHI UNIV +2

Internet-based intelligent fresh-keeping system and method for prefabricated dishes

According to the intelligent preservation system and method for the prefabricated dishes based on the Internet, visual recognition is conducted on the prefabricated dish packaging procedure of a production line, the packaging state information of prefabricated dish products is obtained, and therefore the operation state of the prefabricated dish packaging procedure is adjusted, and the packaging sealing performance of the prefabricated dishes is ensured; and the transportation attribute information of the prefabricated dish product subjected to the packaging process is synchronized to an internet platform, so that remote monitoring is performed on the prefabricated dish product in the transportation process, external environment information and internal environment information of the prefabricated dish product in the transportation process are obtained, and quantitative determination is performed on internal and external environment influences of the prefabricated dish product in the transportation process. According to the method, the refrigeration environment abnormal information of the prefabricated vegetable product in the transportation process is determined, so that the refrigeration and fresh-keeping mode of the refrigeration cabin where the prefabricated vegetable product is located is adjusted, networked real-time refrigeration and fresh-keeping monitoring is carried out on the transportation of the prefabricated vegetable, and it is ensured that the prefabricated vegetable is always stored in a proper refrigeration temperature environment in the whole transportation process.
Owner:GUANGZHOU ZHENGAN FOOD CO LTD +1

Celluloid nitrocotton stability quantitative determination method based on conductivity analysis

PendingCN120971509AMaterial resistanceNitrogen oxidesCelluloid
The invention provides a celluloid nitrocotton stability quantitative determination method based on conductivity analysis, and the method comprises the following steps: determining the conductivity of standard nitrogen oxide gas dissolved in an aqueous solution by adopting a conductivity meter through a standard gas simulation mode according to the criterion of Beckmann-Lockg method 2mL / g; taking the numerical value as a stability index critical value of the celluloid nitrocotton; the method comprises the following steps: absorbing nitrocotton with the same mass by using ultrapure water, heating at 132 DEG C for 2 hours, releasing nitrogen oxide gas, recording the change value of the conductivity in the solution along with time in real time by using an oxidation stability tester, obtaining the change value of the conductivity after 2 hours, and determining the content of nitrogen oxide according to the comparison between the conductivity value and a critical value. And judging whether the stability of the celluloid nitrocotton sample is qualified or not. The method provided by the invention can solve the technical problem that accurate quantitative judgment is difficult to realize due to large sample quantity, high safety risk, tedious operation process and large color change error of test paper observed by human eyes in the traditional method.
Owner:XIAN MODERN CHEM RES INST

Pepsinogen II determination kit and quantitative determination method

The invention provides a pepsinogen II determination kit and a quantitative determination method, the kit comprises a reagent R1 and a reagent R2, the reagent R1 comprises a luminous donor molecule labeled pepsinogen II first antibody and a reaction buffer solution; and the R2 reagent comprises a light-emitting receptor molecule labeled pepsinogen II second antibody and a reaction buffer solution. A double-antibody sandwich structure marked by a luminescent donor and a luminescent receptor respectively is adopted, binding and luminescence can be completed in a liquid phase, solid phase coating and subsequent multi-step washing are not needed, real homogeneous detection and one-step incubation are achieved, the operation process is simplified, the detection time is shortened, and a full-automatic platform is adapted; and an improved buffer system can inhibit non-specific adsorption and reduce heterotropism interference such as RF / HAMA, so that the detection accuracy and repeatability are improved.
Owner:WITTMAN MEDICAL LAB (NANJING) CO LTD

Method for determining haemolysis, icterus and / or lipaemia in a blood sample

PCT designated stage expiredWO2025000001A1Color/spectral properties measurementsSensorsHaemolysisParticle physics
The invention relates to a method for determining haemolysis, icterus and / or lipaemia in a blood sample, in which the blood sample is introduced into a sample vessel (4) and irradiated (3) with near infrared light, after which haemolysis, lipaemia and / or icterus is determined by means of photometric methods. In order to design a method of the aforementioned type such that a quantitative HIL determination is possible in a blood sample without the blood sample being directly manipulated after being filled into the sample vessel or being denaturated by the measurement, it is proposed that analysis classes are defined (1) on the basis of previously recorded reference spectra with a known haemolysis, icterus and / or lipaemia degree, for each analysis class a characteristic spectrum is determined (2) and by means of near infrared reflection spectrometry a sample spectrum of the blood sample, consisting of homogenised whole blood, is detected (6), after which the deviation of the sample spectrum from the characteristic spectrum of each analysis class is determined (7) and the sample spectrum is assigned to that analysis class whose deviation is the smallest, after which a signal assigned to this analysis class is output (8).
Owner:RAMPETSREITER CHRISTOPH

Strawberry cold injury risk early warning and stress detection method and device based on phenotype fusion

The invention discloses a strawberry cold damage risk early warning and stress detection method and device based on phenotype fusion in the technical field of plant cold damage detection, and the method comprises the steps: extracting phenotype space distribution characteristics based on a fluorescence parameter phenotype image and a hyperspectral phenotype image of a to-be-detected leaf with internal electrons in a ground state; calculating the correlation between the leaf pixels of the phenotype spatial distribution characteristics and the physiological indexes of the to-be-detected leaf, obtaining spatial distribution characteristic parameters, inputting the spatial distribution characteristic parameters into an XGBoost integrated learning model, predicting the photosynthetic physiological potential index and the relative negative accumulated temperature of the to-be-detected leaf, and calculating the cold injury risk index result of the to-be-detected leaf; according to the cold injury risk index and the photosynthetic physiological potential index, the strawberry low-temperature cold injury early warning and stress degree is judged. According to the method, spatial variation characteristic information in a specific direction and phenotype data are fully fused, and the cold injury risk early warning level and stress degree of the strawberry plant are rapidly, nondestructively and quantitatively judged from the leaf scale.
Owner:NANJING UNIV OF INFORMATION SCI & TECH

Method for determining element content of metal sample by using direct-reading spectrometer

The invention relates to the technical field of metal spectrum analysis, in particular to a method for determining the element content of a metal sample by using a direct-reading spectrometer. The method comprises the following steps: acquiring a spectrogram of a to-be-analyzed metal sample; further carrying out iterative decomposition on each spectrum peak to obtain a decomposition peak; further inputting the spectrogram and all decomposition peaks of the spectrogram into a spectrum recognition model obtained through self-organizing mapping network training, and recognizing corresponding element categories; and finally, substituting the spectral intensity of each decomposition peak into a pre-established quantitative correction curve of the corresponding element, and calculating to obtain the content of each element in the metal sample without depending on a priori standard spectrogram or a preset overlapping peak number, so that accurate element quantitative determination under a complex overlapping spectrum peak is realized, and the determination precision and adaptability are improved.
Owner:LANZHOU POLYTECHNIC ALLOY POWDER CO LTD

Quantitative quantitative analysis (HPLC) method for isocratic elution of brain-benefiting capsules by quantitative analysis of double components by single marker

The invention relates to an isocratic elution HPLC (high performance liquid chromatography) quantitative determination method for QAMS (quantitative evaluation of double components by single marker) of a brain-benefiting capsule. The method is characterized in that a test solution is obtained by a simple, convenient and rapid pretreatment method, the contents of ganoderic acid A and schizandrin in a sample are simultaneously determined at 255 + / -1nm by adopting a common chromatographic instrument and an isocratic elution mode and taking acetonitrile-methanol-0. 1% phosphoric acid with the volume ratio of 28: 20: 52 as a mobile phase, the wave crest separation is good, the sample injection is performed once, and the peak appearance is completed within 40 minutes. Gradient elution for measuring one component of ganoderic acid A is replaced by common isocratic elution, the content of schizandrin is measured at the same time, and the practical purposes of single-measurement double-evaluation, high efficiency, low consumption and easy popularization are achieved. The method for simultaneously determining ganoderic acid A and schizandrin is reported for the first time, and manifests the novelty and creativity of the technology and the practical value of low cost and double-index detection with half the effort.
Owner:SHIJIAZHUANG ZANGNUO BIOTECH

Method for measuring acidity of all-vanadium electrolyte based on complexing mass difference method

The invention is suitable for the application field of electrolyte acidity determination, and provides a method for determining the acidity of an all-vanadium electrolyte based on a complexing mass difference method, and the method comprises the following steps: taking the all-vanadium electrolyte, and diluting the all-vanadium electrolyte into a pretreated all-vanadium electrolyte with the concentration of 0.05-0.1 mol / L; adding 1.5-2mL of a complexing masking reagent with the molar concentration of 1.5-2mol / L, weighing, and recording as M1; stirring and titrating, weighing and recording as M2; a quantitative determination method of mass difference is used for calculation, and the formula is {(M2-M1)-0.05} / A * C. In conclusion, a mass difference quantitative determination method is adopted, the vanadium complexing masking reagent is added, the adding amount and the pH value of the vanadium complexing masking reagent are controlled, a stable complex is formed through the complexing masking reagent, vanadium ions and impurity ions, the oxidation-reduction reaction and the precipitation reaction of the complex and sodium hydroxide are inhibited, and the accuracy of the acidity value of the all-vanadium electrolyte is improved.
Owner:ENERFLOW TECH CO LTD +1

Method and system for detecting tensile strain mark limit

The invention belongs to the field of mechanical property testing of metal materials, and relates to a method and a system for detecting tensile strain mark limit, the system applies tensile load to a metal plate sample through a test module, an illumination projection module projects a structured light pattern to the surface of the sample, and an image acquisition module continuously acquires surface images of the sample; and the data processing module is used for carrying out wrapped phase calculation, differential phase calculation, distortion parameter extraction and tensile strain mark initiation judgment on the image sequence, so that accurate positioning at the moment of tensile strain mark initiation and objective and quantitative measurement of the critical strain limit are realized. The method can effectively improve the detection sensitivity and accuracy of the material surface instability characteristics, realizes high-precision judgment at the moment of tensile strain mark initiation, and provides a reliable basis for material performance evaluation and quality control.
Owner:SD STEEL RIZHAO CO LTD

Pesticide residue quantitative determination method and system

The invention relates to the field of pesticide residue detection, in particular to a pesticide residue quantitative determination method and system.The method comprises the steps that the retention time of an internal standard substance is obtained, the retention time of to-be-detected pesticide is corrected according to the retention time of the internal standard substance, and the time range of eluent collection is determined according to the retention time of the internal standard substance and the retention time of the to-be-detected pesticide; after uniformly mixing the eluents, obtaining mass spectrograms of the internal standard substance and the pesticide to be detected through a mass spectrometer; determining a ratio of a preset peak area to an actual peak area in a chromatogram output by the detector according to the concentration of the internal standard substance, performing modal decomposition on the chromatogram output by the detector, gradually approaching the ratio to a target value according to a decomposition result, and obtaining an adjusted chromatogram; and correcting the mass spectrum of the pesticide to be detected by using the mass spectrum information of the internal standard substance, and calculating the content of the pesticide to be detected by using the adjusted chromatogram and the corrected mass spectrum.
Owner:SICHUAN JINGZHE AGRICULTURAL CO LTD

Smart NIR II photo-thermal and colorimetric sensing techniques

The invention relates to a method for quantitatively determining the concentration of active myrosinase in a sample. The method comprises the steps of (a) adding a portion of a sample comprising active myrosinase to a first analyte assay formulation for a first period of time to produce an analyte sample, (b) adding a portion of a sample comprising inactivated myrosinase to a second analyte assay formulation for a second period of time to produce an analyte reference sample, and (c) determining the concentration of active myrosinase in the sample. In an embodiment, the first analyte assay formulation includes a near-infrared absorbing and photo-thermal responsive compound. The invention also relates to a formulation for use in a method for the quantitative determination of the concentration of active myrosinase in a sample, as well as to a kit suitable for providing the above formulation.
Owner:NANYANG TECH UNIV

Method for rapidly detecting content of active collagen

The invention relates to the technical field of biological detection signal analysis, and discloses a method for rapidly detecting the content of active collagen. The method comprises the following steps: collecting an original optical response signal of a reaction between a to-be-detected solution and a specific reagent, and constructing a time-space continuous primary reaction track through multi-level slicing and cross-domain splicing of a time domain, a frequency domain and a time-frequency domain; a signal mode set related to activity is separated through convolution kernel group depth scanning. A graph association network between signal modes is constructed based on the set, and a steady-state feature graph representing reaction kinetics is generated through iterative calculation. The feature map is mapped to a virtual reaction space defined by standard data, and quantitative judgment is achieved by calculating the distance between a projection vector and a preset concentration anchor point. According to the method, the sensitivity and the anti-interference performance of detection are enhanced through deep analysis reaction dynamic signals and correlation network modeling.
Owner:FUJIAN YOUJIAN BIOTECHNOLOGY CO LTD

On-site synchronous quantitative determination method for three pathogenic bacteria in marine sediment

The invention relates to an on-site synchronous quantitative determination method for three pathogenic bacteria in marine sediments, and belongs to the technical field of pathogenic bacteria detection.The three pathogenic bacteria are vibrio parahaemolyticus, vibrio alginolyticus and escherichia coli O157, and the method comprises the steps that marine sediment samples are collected and put into a centrifugal tube filled with sterile seawater, and are evenly mixed and centrifuged; taking the supernate as a template for LAMP amplification, respectively adding the supernate into detection tubes of an LAMP reaction system preloaded with the three pathogenic bacteria, and sealing. Placing the detection tube into a detection channel of a portable electronic isothermal gene amplification instrument, and determining a delta C4-t curve, a dv / dt-t curve and Tp; and after the determination is finished, substituting the Tp value into a working curve formula to calculate the content / concentration of the three pathogenic bacteria of the corresponding marine sediment sample in the detection tube. According to the method disclosed by the invention, Bst DNA polymerase and betaine are not needed in the reaction, so that the cost of LAMP analysis is reduced by at least 50%, the operation is simple and convenient, and simultaneous quantitative determination of the three pathogenic bacteria can be completed on site within 1h.
Owner:YELLOW SEA FISHERIES RES INST CHINESE ACAD OF FISHERIES SCI

Synchronous determination method for triazole pesticides in feed

PendingCN119125380AAccurate and synchronized measurement methodEasy extractionComponent separationFlusilazoleSolid phase extraction
The invention relates to a synchronous determination method for triazole pesticides in a feed. The method comprises the following steps: S1, preparing a matrix matching series of standard working solutions; s2, extracting a to-be-detected feed sample with acetonitrile to obtain a to-be-purified solution; s3, enabling the to-be-purified solution to completely pass through a Captiva EMR-Lipid solid-phase extraction column, collecting all effluent, fixing the volume by using acetonitrile, uniformly mixing, and enabling the mixture to pass through a microfiltration membrane, so as to obtain a to-be-detected sample solution; and S4, carrying out high performance liquid chromatography-tandem mass spectrometry determination on the matrix matching series standard working solution and the to-be-detected sample solution, and quantifying each to-be-detected medicine by adopting an external standard method. The method provided by the invention is good in extraction and purification effect and high in sensitivity, can realize accurate qualitative and quantitative determination of chlorazophos, tebuconazole, tebuconazole, diniconazole, iminazole, flusilazole and tetraconazole residues in a feed sample with relatively high extraction efficiency and relatively low time cost and economic cost, and can provide technical support for feed quality safety detection.
Owner:重庆市动物疫病预防控制中心(重庆市兽药饲料检测所) +1

Earthquake prediction method and system for igneous rock intrusive fault zone closure

The invention belongs to the technical field of exploration and development of fault block oil and gas reservoirs, and particularly relates to a seismic prediction method and system for igneous rock intrusive fault zone sealing performance, and the method comprises the steps: determining the seismic attribute and seismic frequency attribute of a highlight body of a target interval according to petroleum seismic exploration data; determining magma invasion characteristic parameters according to the seismic attribute and the seismic frequency attribute of the highlight body, and determining fracture characteristic parameters of the fracture zone according to an eigenvalue characteristic algorithm operation result in the third-generation coherent attribute of the seismic data; determining an internal structure index of the intrusive fault zone according to the magma invasion characteristic parameters and the fracture characteristic parameters of the fault zone; and comparing the internal structure index of the intrusive fault zone with a closed lower limit threshold of the internal structure index of the known oil reservoir fault zone, and predicting the fault zone closure and the closure capability thereof according to a comparison result, thereby realizing quantitative determination of the oil and gas migration and dredging capability of the fault with magma invasion in the clastic rock stratum.
Owner:PETROCHINA CO LTD

Multi-source operation exception association analysis and grading processing method and system

The invention discloses a multi-source operation exception association analysis and grading disposal method and system. The method comprises the steps of telemetry data collection and caching, non-blocking sending and self-adaptive readaptation, sliding time window and matrix construction, exception association clustering and comprehensive scoring, exception type judgment and grading alarm and self-healing disposal. The method comprises the following steps: continuously sensing a program execution state and an abnormal behavior, realizing data peak clipping storage by local cache, introducing a self-adaptive backoff retry mechanism, decentralizing transmission of abnormal data on the premise of not blocking a main service, and identifying and distinguishing anomalies in combination with abnormal propagation characteristics and an influence coupling relationship. And after quantitative judgment, automatically outputting a matched grading treatment strategy and an operation recovery measure. The closed-loop operation treatment mechanism of coverage sensing, transmission, aggregation, judgment and disposal constructed by the invention can realize rapid sensing, accurate attribution and adaptive response of the abnormity in a complex industrial load environment, and the stability and reliability of the operation of an industrial system are remarkably improved.
Owner:NARI TECH CO LTD +1

Interferometer-based synthetic multi-exposure speckle imaging (symesi) method and system

A SyntheticMulti-Exposure Speckle Imaging (syMESI) methodology necessarily utilizing an optical interferometer apparatus as part of the speckle imaging system to overcome the optical detector noise that conventionally limits the reliable and accurate determination of a speckle contrast characteristic at low exposure times. The use of such methodology enabled a quantitative determination of absolute value(s) of changes of motion at the target object (such as blood flow changes in tissue) at low photon budget of less than 40 counts of average detection intensity and / or quantitative imaging of the blood flow at the object in interoperative setting with a low-cost camera sensor.
Owner:UNIV OF SOUTH FLORIDA

Method and system for analysing and calculating aggregation of analytes in chemical compound

PCT designated stage expiredWO2025113638A1Image enhancementImage analysisAnalyteTest sample
The present disclosure relates to the field of chemical analysis technology, and specifically discloses methods and systems for analyzing and calculating aggregation of analytes in a chemical compound. The analysis method includes: acquiring a chemical image of analytes in a control sample and a chemical image of the analytes in at least one test sample, performing Gaussian blur on each of the chemical images, and performing brightness calibration on the obtained Gaussian blurred image; converting the brightness calibrated Gaussian blurred image into a binary image; converting the binary image into one-dimensional array; calculating a continuous length of predetermined pixels in a one-dimensional array and a frequency corresponding to each continuous length, and generating a histogram of frequency and continuous length; fitting the histogram with an exponential Gaussian distribution function to obtain an exponential attenuation parameter of the fitted exponential Gaussian distribution function; calculating an aggregation index of the test sample according to the exponential attenuation parameters corresponding to the test sample and the control sample. This technical solution can quantitatively determine the aggregation of analytes.
Owner:ELC MANAGEMENT LLC

Three-dimensional confocal X-ray emission-fluorescence bimodal spectrometer and use method thereof

The invention relates to the technical field of optical equipment, in particular to a three-dimensional confocal X-ray emission-fluorescence bimodal spectrometer and a use method. X-ray tube focus point source excitation and three-dimensional confocal light path design are adopted to generate a bimodal signal source, and X-ray emission and X-ray fluorescence bimodal signals of chemical elements in a sample are respectively collected and obtained through a position sensitive detector and an energy sensitive detector through a confocal bent crystal focusing light splitting system and a fluorescence light path; confocal scanning of a three-dimensional space in a sample is completed through a sample loading and position self-control system, and the absolute content of a chemical element in a specific form is calculated through a signal linkage analysis module. The spectrometer can realize synchronous quantitative determination and analysis of forms and contents of chemical elements, solves the problems that XRF and XES can only be operated step by step, form proportion and total data cannot be associated in real time, and an XES spectrogram has spectrum peak overlapping interference and the like, and has one-dimensional point determination, two-dimensional surface scanning and three-dimensional body analysis capabilities.
Owner:NAT RESERACH CENT OF GEOANALYSIS