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1086 results about "Quantitative determination" patented technology

Quantitative analysis refers to the determination of how much of a given component is present in a sample. The quantity may be expressed in terms of mass, concentration, or relative abundance of one or all components of a sample.

Method for analyzing remaining oil distribution of fractured-vuggy reservoir

The invention provides a method for analyzing remaining oil distribution of a fractured-vuggy reservoir, belonging to the fields of numerical reservoir simulation and oil-gas field development. In the method, a complex medium consisting of a cave medium, a crack medium and a pore medium is partitioned into a plurality of space unit blocks in a space field; each block consists of V, F and M units which represent a cave, a crack and a substrate in the block respectively and constitute a V-F-M model; the flow of a multi-phase fluid in the complex medium is described by the motion of a fluid among the units in each block and the motion of a fluid among the units of different blocks; and the flow of the fluid among the units can be considered as infiltration flow, pipe flow or laminar flow between parallel walls, Darcy flow or non-Darcy flow. According to the method, scientific description and accurate numerical simulation of the fractured-vuggy reservoir are realized, and technical foundations are laid for the alignment of the remaining oil distribution position of the fractured-vuggy reservoir with a numerical simulation technology, quantitative determination of the reserves abundance of the reservoir, scientific and reasonable development of oil fields provided with the reservoir and final increase in the recovery ratio.
Owner:CHINA PETROLEUM & CHEM CORP +1

Method for producing fluorescent microballoons immune chromatography test paper stripe and quantitative determination method

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.
Owner:江西中德生物工程股份有限公司

Comprehensive quantitative determination method for grouting reinforcement effect of underground engineering crushed surrounding rocks

The invention discloses a comprehensive quantitative determination method for grouting reinforcement effect of underground engineering crushed surrounding rocks. The method is based on four technological detection meanings, and the four meanings comprise surrounding rock boring coring, detection by a ground penetrating radar, borehole inspection detection and in-situ strength detection on bored surrounding rock. By using the four detection meanings for respective statistics and analysis, the surrounding-rock boring coring enhancement rate, the damage area reinforcing enhancement rate, the crack closing enhancement rate, the strength parameter enhancement rate and other parameters before or after grouting are obtained. The grouting reinforcement effect is subjected to comprehensive quantitative analysis through a weight analysis method, and a quantitative evaluation index for the grouting reinforcement effect is established, and scientific reasonable evaluation is performed on the grouting reinforcement effect of the underground engineering crushed surrounding rock. The comprehensive quantitative determination method for the grouting reinforcement effect of underground engineering crushed surrounding rocks overcomes the disadvantages that a conventional technology for the grouting reinforcement effect of underground engineering crushed surrounding rocks is behindhand in detection meaning and single in evaluation method, cannot realize quantization and the like. The provided comprehensive quantitative determination method is advanced in detection meaning, relatively novel and relatively scientific compared with a conventional experiential qualitative analysis method.
Owner:SHANDONG UNIV +1

Method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules

The invention relates to a method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules. The peroxidase simulated by manganese dioxide nanosheet can perform catalytic oxidation on substrates of 3,3',5,5'-tetramethyl benzidine TMB, 2,2-azino-di(3-ethyl-benzothiazoles-6-sulfonic acid) diammonium salt ABTS and o-phenylenediamine OPD, and changes the color from colorless to blue, green and orange respectively, at the same time, the manganese dioxide nanosheet can sensitively and selectively perform an oxidation reduction reaction with reductive biological molecules such as glutathione and ascorbic acid, oxidation product concentration of the substrates such as TMB, ABTS and OPD is changed, and then, the reductive biological molecules such as glutathione and ascorbic acid are subjected to quantitative determination through a colorimetric analysis method. The method has the characteristics of simple operation, high sensitivity, good reappearance and high selectivity; a detection linear scope of glutathione is 1-15 [mu]M, the detection limit is 0.3 [mu]M; the detection linear scope of ascorbic acid is 3-100 [mu]M, the detection limit is 0.8 [mu]M; and the method can be used for detecting various phenolic compounds.
Owner:XI AN JIAOTONG UNIV

Homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and kit used for method

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.
Owner:TIANJIN NANKAI HOSPITAL

Nano gold biological composite probe, detection method and application thereof

The invention relates to a nano gold biological composite probe, a detection method and application thereof. The detection method is characterized in that: the method comprises the following steps: firstly marking a bead through a monoclonal antibody of a protein to be tested; marking the polyclonal antibody of the protein to be tested on nano gold while and simultaneously marking a DNA probe with a biotin label; carrying out a biotin-streptavidin reaction on the DNA probe on the nano gold to make a lanthanide bonded with colloidal gold to construct the nano gold biological composite probe; mixing the bead of marked monoclonal antibody of the protein to be tested, the nano gold biological composite probe and a protein sample to be tested, carrying out the incubation on the mixture for a certain time at 37 DEG C; cleaning away the nano gold probe which does not react; adding a reinforcing liquid; and determining the fluorescence intensity so as to realize the aim of carrying out the quantitative determination on the protein to be tested. The method obviously improves the detection sensitivity of biomolecules, and is used for detecting a plurality of kinds of biomolecules synchronously. The method is widely applied in the fields of clinical diagnosis, antigen, antibody and nucleic acid detection, health quarantine, environmental tests, and the like.
Owner:SHANGHAI INST OF MICROSYSTEM & INFORMATION TECH CHINESE ACAD OF SCI

Method for quantitatively measuring inhomogeneous deformation of nano-crystal material

The invention relates to a method for quantitatively measuring the inhomogeneous deformation of a nano-crystal material. The method comprises the following steps: firstly processing the measured nano-crystal material into a stretching sample, selecting a marking area on the stretching sample and marking the stretching sample; secondly, putting the stretching sample into a mechanics testing machine, and loading the stretching sample under the condition that the quasistatic strain rate of a displacement control mode at room temperature is 10<-4>s<-1>; obtaining data and displaying a stress strain curve by a material testing system of the mechanics testing machine, adopting a digital camera to acquire the image on the surface of the sample at the same time of loading, and recording the image by connecting the digital camera with a computer; and finally, carrying out digital image processing on the image acquired by the digital camera in the process of stretching to obtain the surface displacement and a stain field image of the tested image in the process of stretching. The method measures the cause of formation of a shear zone in a simple mode with low cost, so the method can have wide application prospect in the fields of modern mechanics, microelectronic technology, ultraprecise processing technology, nanometer technology, and the like.
Owner:NANJING UNIV OF TECH

Staphylococcus aureus unmarked electrochemical aptamer sensor based on reduced graphene oxide-nanogold composite material

The invention discloses a method for detecting staphylococcus aureus based on reduced graphene oxide-nanogold composite material in combination with aptamer, belonging to the field of electrochemistry. According to the basic principle of the method, the reduced graphene oxide-nanogold composite material is used to modify a sulfhydrylation whole staphylococcus aureus capture probe by a layer by layer self-assembly method. When the probe is incubated in staphylococcus aureus liquid, effective binding sites of the probe can be bound with the staphylococcus aureus to wrap the staphylococcus aureus in a three-dimensional space structure, thus, the whole capture to target bacteria is realizes. The electron transmission on the surface of an electrode is blocked after the target bacteria is bound, so that a electrochemical resistance value increases, and quantitative determination on the staphylococcus aureus can be realized based on the change of the resistance value. By virtue of excellent electron transmission capability of graphene and large specific surface area of nanogold, detection signals can be amplified, and detection time can be shortened. The new method provided by the invention can be applied to whole bacteria detection on the staphylococcus aureus, can expand application of impedance-based electrochemical unmarked detection, and has the advantages of strong specificity, high sensitivity, cheapness, rapidness, and so on.
Owner:JIANGNAN UNIV

High performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics

The invention relates to the field of analytical chemistry and food safety, and provides a high performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics. A cosmetic sample of 0.4g is weighed and placed in a plastic centrifuge tube of 50 ml, sample extraction liquid of 20ml is added, after the cosmetic sample of 0.4g and the sample extraction liquid of 20ml are mixed for 2 minutes in a vortex mode, sonic oscillation extraction is carried out for 20 minutes, 8000 revolutions per minute centrifugation is carried out for 10 minutes and, and supernate is to be purified; and after test solutions sequentially pass through microfiltration membranes of 0.45 micrometer and 0.20 micrometer, a high performance liquid chromatograph is adopted to carry out qualitative determination and quantitative assay. The detection method has the advantages of being simple, fast, accurate, efficient, and the like, and can detect 12 ultraviolet absorbents at the same time. Technical indexes of detection minimum, the recovery rate, the degree of precision, and the like all meet relevant demands for ultraviolet absorbent detection in sun block cosmetics at home and abroad. The method is suitable for detection and monitoring of the ultraviolet absorbents in the sun block cosmetics, and has great significance on promoting of import and export trade of cosmetics of our country, ensuring of safety of the cosmetics, and guarantee of human body health.
Owner:林维宣

Environmental aerosol direct sampling gathering sample injecting device and quantitative analysis method

ActiveCN103487494ACondensation growth effect is goodRealize online quantitative analysisSamples introduction/extractionMaterial analysis by electric/magnetic meansInjection volumeQuantitative determination
The invention discloses an environmental aerosol direct sampling gathering sample injecting device and a quantitative analysis method. The environmental aerosol direct sampling gathering sample injecting device comprises an environmental aerosol sampling module, an aerosol atomization dissolving generation module, an aerosol condensation growing up module, an aerosol number concentration gathering module and a base body gas removing module. The environmental aerosol sampling module comprises a sampling port, a sampling pump and a connection pipeline, preferably, the sampling pump is a membrane pump; the aerosol atomization dissolving generation module comprises an atomizer, a fog chamber, a drying device and a connection pipeline, the atomizer is used for atomizing a standard solution to generate fog drops, the fog room is used for removing large fog drops and the drying device is used for dissolving the fog drops output from the fog room and removing steam contained in the fog drops. Due to the mode that the environmental aerosol direct sampling gathering sample injecting device serves as an aerosol sample injection system and is used in cooperation with an ICP-MS, quality sample injection rate of an element to be measured in environmental aerosol can be measured quantitatively, and then sample injection volume flow of the environmental aerosol is divided by the quality sample injection rate to acquire the quality volume concentration of the element to be measured.
Owner:NORTHWEST INST OF NUCLEAR TECH

Electrochemical system for analyzing performance and properties of electrolytic solutions

The invention relates to the analysis of the performance and properties of electrochemical processes, and specifically, to electrolytic solutions and electrode processes. The invention discloses a device and a method for obtaining qualitative and quantitative information for the kinetics of the electrode reactions, the transport processes, the thermodynamic properties of the electrochemical processes taking place in the cell. When a deposition reaction takes place, the device provides also valuable information about the relationship between the current density and deposit properties including but not limited to the deposit color, luster, and other aspects of its appearance. The device disclosed herein typically is comprised of a multiplicity of cathodic or anodic regions where one or more electrochemical reactions take place simultaneously, but at a different rate. From the precisely measured segmental currents one can obtain among other process properties: (1) An accurate relationship between the deposit appearance and the current density. This relationship can be used for process diagnostics, troubleshooting, control of concentrations, pH, and additives and contaminants and for optimizing the operating conditions, including the voltage, current, and circulation rate. (2) Quantitative determination of important process parameters including but not limited to, kinetics (e.g., exchange current density, cathodic and anodic transfer coefficients), transport (e.g. conductivity), and thermodynamics (e.g., standard potential). A particularly attractive application of the process is for the quantitative and qualitative processes of alloys plating and for the determination of the relationship between the current efficiency and the applied current density.
Owner:LANDAU UZIEL

Portable electrostatic detection device and electrostatic detection method thereof

ActiveCN102353855ASolving Quantitative Detection ProblemsEasy to follow upElectrical measurementsElectricityQuantitative determination
The invention discloses a portable electrostatic detection device and an electrostatic detection method thereof and belongs to the field of detection. A static inductive sensor of the portable electrostatic detection device comprises a shield casing with a signal receiving window; an electrostatic signal receiving surface is arranged inside the casing; a circuit board and a signal transmitting bracket are arranged in the casing; a vibration electrode slice with a vibration source is arranged on the signal transmitting bracket; a covering sheet in parallel to a metallic electrostatic signal receiving surface is arranged at the front end of the vibration electrode slice; the rear end of the vibration electrode slice is fixedly arranged on the signal transmitting bracket or fixedly arranged on the signal transmitting bracket through the vibration source; and an electrical signal processing unit of the portable electrostatic detection device at least comprises a vibration source driving circuit, a signal amplifying circuit, a rectifier circuit and a singlechip circuit. According to the portable electrostatic detection device and the electrostatic detection method thereof disclosed by the invention, the induced electrostatic voltage is converted into alternating voltage by adopting a mechanical interrupt method for an output and subsequent detection/amplifying unit to use, and thus the electrostatic quantitative determination is realized; and in addition, the repeatability of monitored and detected data is favorable and the precision and the stability of the electrostatic detection are improved.
Owner:上海安平静电科技有限公司

Fourier transform chip spectrometer based on integrated light technique

ActiveCN102207459ASensitive assayFast and Sensitive Absorption SpectroscopyPhase-affecting property measurementsColor/spectral properties measurementsModulation functionLighting spectrum
The invention discloses a Fourier transform chip spectrometer based on integrated light technique, comprising an integrated optical waveguide chip having electro-optic modulation function. Light from a light source enters the integrated optical waveguide chip through a fiber transmission coupler, and waveguide light from the chip enters an optical waveguide interferometer through a sensitive window interval. The interferometer outputs signals to a photoelectric detector. A voltage function generator is used for applying voltages varying with time between two modulated electrodes of the optical waveguide interferometer, the photoelectric detector is used for real-time measuring the change of the signal strength of the interferometer varying with the modulated voltage, and a signal processing chip connected with the photoelectric detector and the voltage function generator at same time is used for rapidly processing Fourier transform on the signals of the interferometer to obtain an incident light spectrum. The Fourier transform chip spectrometer provided by the invention can determine visible-infrared light absorbing spectra of solids, liquid, even monomolecular adsorption layers in the sensitive window, and determine fluorescence spectra of fluorescent substances in the sensitive window, with the advantages of strong anti-interference capability, and is suitable for rapid on-site quantitative determination.
Owner:INST OF ELECTRONICS CHINESE ACAD OF SCI
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