1086 results about "Quantitative determination" patented technology

The invention provides a biosensor comprising a support; a conductive layer composed of a electrical conductive material such as a noble metal, for example gold or palladium, and carbon; slits parallel to and perpendicular to the side of the support; working, counter, and detecting electrodes; a spacer which covers the working, counter, and detecting electrodes on the support; a rectangular cutout in the spacer forming a specimen supply path; an inlet to the specimen supply path; a reagent layer formed by applying a reagent containing an enzyme to the working, counter, and detecting electrodes, which are exposed through the cutout in the spacer; and a cover over the spacer. The biosensor can be formed by a simple method, and provides a uniform reagent layer on the electrodes regardless of the reagent composition.

The present invention is directed to the development of a biosensor based on the immuno-chromatographic method that can provide an assay speed and convenience required for point-of-care (the doctor's office and emergency room) testing or home-version diagnosis. Though certain physical symptoms, such as pregnancy and ovulation, or bacterial infection may be identified by a qualitative analysis for the presence of indicating substances, most analytes for clinical investigation demand their concentrations known in specimens. Therefore, the inventors of the present invention have developed a novel biosensor by combining the immuno-chromatographic method and the electric conductivity detection technology so that on-site quantitative determination at the points of care or at home may be carried out.

The present invention relates to a simple immunochemical semi-quantitative assay method according to chromatography, which comprises trapping a certain amount of an analyte in a sample with a predetermined amount of a fixed antibody for the analyte before qualitative analysis of the analyte, the certain amount corresponding to the amount of the fixed antibody, and thereby decreasing a concentration of the analyte to be subjected to subsequent immunochemical qualitative determination, and an apparatus therefor.

The invention provides a method for analyzing remaining oil distribution of a fractured-vuggy reservoir, belonging to the fields of numerical reservoir simulation and oil-gas field development. In the method, a complex medium consisting of a cave medium, a crack medium and a pore medium is partitioned into a plurality of space unit blocks in a space field; each block consists of V, F and M units which represent a cave, a crack and a substrate in the block respectively and constitute a V-F-M model; the flow of a multi-phase fluid in the complex medium is described by the motion of a fluid among the units in each block and the motion of a fluid among the units of different blocks; and the flow of the fluid among the units can be considered as infiltration flow, pipe flow or laminar flow between parallel walls, Darcy flow or non-Darcy flow. According to the method, scientific description and accurate numerical simulation of the fractured-vuggy reservoir are realized, and technical foundations are laid for the alignment of the remaining oil distribution position of the fractured-vuggy reservoir with a numerical simulation technology, quantitative determination of the reserves abundance of the reservoir, scientific and reasonable development of oil fields provided with the reservoir and final increase in the recovery ratio.

The subject invention relates to a set of DNA primers which, when utilized in conjunction with the polymerase chain reaction (PCR) assay, can amplify and speciate DNA from five medically important Candida species. Furthermore, the PCR amplified products, generated by the primers, can also be used to create species specific probes which can also detect and confirm the five species of Candida. Thus, the present invention allows for early diagnosis and treatment of an infection. The assay is useful in the context of monitoring antifungal treatment regimens, screening potential antifungal agents, and similar applications requiring quantitative determinations.

The invention discloses a method for preparing fluorescent microspheres immunochromatographic test paper strip and quantitative detection method. The invention takes the luminous nano-particles of dual-structure silicon dioxide compound organic dye as a marker, uses the immunochromatographic technology for preparing fluorescent microspheres immunochromatographic test paper strip, and then prepares a detection card which consists of a sample pad, a glass fiber membrane, a nitrocellulose membrane and absorbent paper, wherein the nitrocellulose membrane is fixedly provided with a detection line and a quality control line. In the detection process, the best excitation light souce of fluorescent microspheres is used for excitation; after the emitted fluorescence passes through a filter, a CCD scanning technology or fiber-optic technology is used for collecting, accumulating or multiplicating the emitted spectra which is then converted into a numerical signal; then the measured fluorescence intensity of the detection line is multiplied by a correction coefficient, and later the corrected fluorescence intensity is substituted in a standard curve which is preset in a fluorescence analyzer; and finally, the concentration of an object to be measured in the sample can be automatically calculated and obtained by the fluorescence analyzer. The invention has high sensitivity, accurate quantization and easy operation.

Novel chemical compounds, with application in fluorometric analytical methods, for qualitative and quantitative determination of biomolecules. The aim of the invention is to identify and prove the suitability of such compounds. Said aim is achieved with compounds of formula (1) where R′ is an antenna function, R² is a chelate forming agent, containing a coordinated lanthanide(III)ion, X is —OH or a group with affinity for the biomolecule, bonded to a carboxylate group of the chelate forming agent by means of an amide bond and Y is —H or a group with affinity for the biomolecule, coupled to the antenna function.

A method for quantitatively assaying one or more target molecules in a sample uses a nucleic acid aptamer that is specific for each target molecule. A quantitative replicative procedure is used to determine a quantity of aptamer specific for each molecule.

An efficient design for an expanded dynamic range in a lateral flow one step assay for the detection of an analyte in a biological sample is disclosed. The device comprises a multiple strip design, each constructed of four zones; a sample receiving zone, a sample treatment zone, a labeling zone, and a capture zone. The sample containing analyte is accepted in the sample receiving zone in the form of blood, serum, plasma, or urine. It is then carried into the sample treatment zone where it is rendered compatible with the chemistries of the assay strip. The treated sample then flows into the labeling zone where it interacts with visible particles that are coupled to analyte specific binding proteins. The flow continues, carrying the labeled analyte into the capture zone where it is immobilized in specific regions with analyte specific binding proteins. Excess flow is absorbed in an absorbent zone that is in contact with the capture zone. A positive result is interpreted by detection of the visible particles in the specified regions of the capture zone.

An analyte test element for the qualitative and/or quantitative determination of at least one analyte in a physiological or aqueous sample fluid having a first surface (2a) and a second surface (4a) in a predetermined distance opposite from each other, said both surfaces are provided with two substantially equivalent patterns forming areas of high and low surface energy which are aligned mostly congruent, whereby the areas of high surface energy (6, 6′) create a sample distribution system with at least two detection areas (6a, 6′a), said at least one of the detection areas (6a, 6′a) of the first and second surfaces (2a, 4a) is provided with at least one non-enzymatic recognition element (32). The analyte test element is suitable for analyte test systems evaluating the affinity reaction between an analyte of interest and a recognition element and therefore provides a suitable test system to perform immunoassays, receptor-assays, or other affinity assays with a simple test element containing qualitative or quantitative calibration mechanisms suitable for point of care and home settings.

A device and process for preventing medical errors due to the improper administration of an intravenously delivered medication includes the spectroscopic analysis of intravenous fluid components. An emission source and detector are placed adjacent to the intravenous tubing of an administration set to generate signals for spectroscopic analysis. The signals are processed to identify the medication and, in certain embodiments of the invention, can determine the medication's concentration. In a preferred embodiment, the emission source, detector, and hardware and software for the spectroscopic analysis are placed in an infusion pump.

The invention provides a stress measuring device with higher precision, which can measure the size, direction and distribution of the stress of optical glass. The stress measuring device of the optical glass comprises a communicated light path interferometer and a stander, wherein the stander comprises a frame and a plane scanning test platform arranged on the frame; and a laser receiving unit and a laser emitting unit of the communicated light path interferometer are arranged on the stander, and can synchronously move front and back, and up and down. The stress measuring device achieves the quantitive measurement of the stress size and the stress uniformity of the optical glass; in addition, the precision is greatly improved, the resolution reaches 0.01nm, and the size of the measurable optical glass is greatly widened. The stress measuring device can better measure the stress optical coefficient of the optical glass; and compared with the other polarized stress meter measuring devices with weight application, the measuring precisions of the pressure and the stress of load are obviously improved, and the measuring precision of the stress optical coefficient can reach 0.03*10<-12>/Pa.

The invention relates to an optochemical sensor functioning in accordance with the FRET-principle and exhibiting an acceptor (chromophore or luminophore) responsive to an analyte contained in a sample medium as well as a donor (luminophore), characterized in that acceptor and donor are located in separate chemical phases, whereby the phase containing the donor is essentially impermeable to the sample medium or to components of the sample medium affecting the luminescence characteristics of the luminophore. The invention further relates to a method for qualitative and/or quantitative determination of at least one analyte and/or component of a gaseous or liquid measuring medium according to the FRET-principle, characterized by the use of the sensor according to the invention.

The invention discloses a comprehensive quantitative determination method for grouting reinforcement effect of underground engineering crushed surrounding rocks. The method is based on four technological detection meanings, and the four meanings comprise surrounding rock boring coring, detection by a ground penetrating radar, borehole inspection detection and in-situ strength detection on bored surrounding rock. By using the four detection meanings for respective statistics and analysis, the surrounding-rock boring coring enhancement rate, the damage area reinforcing enhancement rate, the crack closing enhancement rate, the strength parameter enhancement rate and other parameters before or after grouting are obtained. The grouting reinforcement effect is subjected to comprehensive quantitative analysis through a weight analysis method, and a quantitative evaluation index for the grouting reinforcement effect is established, and scientific reasonable evaluation is performed on the grouting reinforcement effect of the underground engineering crushed surrounding rock. The comprehensive quantitative determination method for the grouting reinforcement effect of underground engineering crushed surrounding rocks overcomes the disadvantages that a conventional technology for the grouting reinforcement effect of underground engineering crushed surrounding rocks is behindhand in detection meaning and single in evaluation method, cannot realize quantization and the like. The provided comprehensive quantitative determination method is advanced in detection meaning, relatively novel and relatively scientific compared with a conventional experiential qualitative analysis method.

The invention relates to a method for simulating peroxidase by manganese dioxide nanosheet for detection of reductive biological molecules. The peroxidase simulated by manganese dioxide nanosheet can perform catalytic oxidation on substrates of 3,3',5,5'-tetramethyl benzidine TMB, 2,2-azino-di(3-ethyl-benzothiazoles-6-sulfonic acid) diammonium salt ABTS and o-phenylenediamine OPD, and changes the color from colorless to blue, green and orange respectively, at the same time, the manganese dioxide nanosheet can sensitively and selectively perform an oxidation reduction reaction with reductive biological molecules such as glutathione and ascorbic acid, oxidation product concentration of the substrates such as TMB, ABTS and OPD is changed, and then, the reductive biological molecules such as glutathione and ascorbic acid are subjected to quantitative determination through a colorimetric analysis method. The method has the characteristics of simple operation, high sensitivity, good reappearance and high selectivity; a detection linear scope of glutathione is 1-15 [mu]M, the detection limit is 0.3 [mu]M; the detection linear scope of ascorbic acid is 3-100 [mu]M, the detection limit is 0.8 [mu]M; and the method can be used for detecting various phenolic compounds.

The invention provides a homogeneous luminescence immunoassay method for quantitatively analyzing multiple components simultaneously and a kit used for the method. Receptor microspheres containing various different fluoresceins are adopted, and antibody molecules for capturing different biological markers to be measured are enveloped in the method; in a measuring process, the multiple biological markers in a sample to be measured are combined with the corresponding antibody molecules on the surfaces of the receptor microspheres and biotinylated antibodies in a detection system respectively to form double-antibody sandwich compositions which are respectively connected with donor microspheres labeled with streptavidin; when the donor microspheres are irradiated by exciting light, all types of the receptor microspheres send out optical signals with different wavelengths; the intensities of the light with different wavelengths are respectively detected, so that the biological markers to be measured can be accurately quantified. The kit comprises the receptor microspheres containing chemiluminescence reagents and the fluoresceins, the biotinylated antibodies and the donor microspheres containing photosensitive substances. The homogeneous luminescence immunoassay method and the kit have the beneficial effects that simultaneous and quantitative measurement on multiple components is realized, and the detection cost is reduced.

The invention relates to a nano gold biological composite probe, a detection method and application thereof. The detection method is characterized in that: the method comprises the following steps: firstly marking a bead through a monoclonal antibody of a protein to be tested; marking the polyclonal antibody of the protein to be tested on nano gold while and simultaneously marking a DNA probe with a biotin label; carrying out a biotin-streptavidin reaction on the DNA probe on the nano gold to make a lanthanide bonded with colloidal gold to construct the nano gold biological composite probe; mixing the bead of marked monoclonal antibody of the protein to be tested, the nano gold biological composite probe and a protein sample to be tested, carrying out the incubation on the mixture for a certain time at 37 DEG C; cleaning away the nano gold probe which does not react; adding a reinforcing liquid; and determining the fluorescence intensity so as to realize the aim of carrying out the quantitative determination on the protein to be tested. The method obviously improves the detection sensitivity of biomolecules, and is used for detecting a plurality of kinds of biomolecules synchronously. The method is widely applied in the fields of clinical diagnosis, antigen, antibody and nucleic acid detection, health quarantine, environmental tests, and the like.

An apparatus and a method that can be used for qualitative or quantitative determination of the three-dimensional location of two bodies relative to one another which can be used, for example, to determine the mutual position of two bodies according to angular or translational coordinates. Furthermore, the apparatus and method can be used in a measurement robot or in a coordinate measurement device. The device composed of a device for emitting a light beam which is fanned in several planes and at least three, preferably four or more, optoelectronic line sensors or linear sensors for determination of the incidence points of the light beam which has been flared in several planes on the line sensors or an upstream target surface.

The invention relates to a method for quantitatively measuring the inhomogeneous deformation of a nano-crystal material. The method comprises the following steps: firstly processing the measured nano-crystal material into a stretching sample, selecting a marking area on the stretching sample and marking the stretching sample; secondly, putting the stretching sample into a mechanics testing machine, and loading the stretching sample under the condition that the quasistatic strain rate of a displacement control mode at room temperature is 10<-4>s<-1>; obtaining data and displaying a stress strain curve by a material testing system of the mechanics testing machine, adopting a digital camera to acquire the image on the surface of the sample at the same time of loading, and recording the image by connecting the digital camera with a computer; and finally, carrying out digital image processing on the image acquired by the digital camera in the process of stretching to obtain the surface displacement and a stain field image of the tested image in the process of stretching. The method measures the cause of formation of a shear zone in a simple mode with low cost, so the method can have wide application prospect in the fields of modern mechanics, microelectronic technology, ultraprecise processing technology, nanometer technology, and the like.

The invention discloses a determination method of a urine metabolic marker for early diagnosis of diabetic nephropathy. The method mainly utilizes analysis technique and method such as gas mass spectrometry and mass liquid spectrometry for quantitative determination of endogenous small molecule compounds in urine of patients with diabetes and diabetic nephropathy; and relative concentration difference between endogenous small molecule compounds in a pathological group and a normal group is calculated and compared, so as to be applied to clinical early diagnosis of diabetic nephropathy. Compared with other existing clinical diagnosis indexes, the method has advantages of wide adaptation range, sensitivity, simple sampling and operation, and no harm on the body. The method is more suitable for screening of diabetic nephropathy in early stage with some uncertain physiological and biochemical indexes, so as to avoid missing of the best treatment time due to delayed diagnosis.

The invention discloses a method for detecting staphylococcus aureus based on reduced graphene oxide-nanogold composite material in combination with aptamer, belonging to the field of electrochemistry. According to the basic principle of the method, the reduced graphene oxide-nanogold composite material is used to modify a sulfhydrylation whole staphylococcus aureus capture probe by a layer by layer self-assembly method. When the probe is incubated in staphylococcus aureus liquid, effective binding sites of the probe can be bound with the staphylococcus aureus to wrap the staphylococcus aureus in a three-dimensional space structure, thus, the whole capture to target bacteria is realizes. The electron transmission on the surface of an electrode is blocked after the target bacteria is bound, so that a electrochemical resistance value increases, and quantitative determination on the staphylococcus aureus can be realized based on the change of the resistance value. By virtue of excellent electron transmission capability of graphene and large specific surface area of nanogold, detection signals can be amplified, and detection time can be shortened. The new method provided by the invention can be applied to whole bacteria detection on the staphylococcus aureus, can expand application of impedance-based electrochemical unmarked detection, and has the advantages of strong specificity, high sensitivity, cheapness, rapidness, and so on.

The invention relates to the field of analytical chemistry and food safety, and provides a high performance liquid chromatography detection method for residual quantity of multiple ultraviolet absorbents in cosmetics. A cosmetic sample of 0.4g is weighed and placed in a plastic centrifuge tube of 50 ml, sample extraction liquid of 20ml is added, after the cosmetic sample of 0.4g and the sample extraction liquid of 20ml are mixed for 2 minutes in a vortex mode, sonic oscillation extraction is carried out for 20 minutes, 8000 revolutions per minute centrifugation is carried out for 10 minutes and, and supernate is to be purified; and after test solutions sequentially pass through microfiltration membranes of 0.45 micrometer and 0.20 micrometer, a high performance liquid chromatograph is adopted to carry out qualitative determination and quantitative assay. The detection method has the advantages of being simple, fast, accurate, efficient, and the like, and can detect 12 ultraviolet absorbents at the same time. Technical indexes of detection minimum, the recovery rate, the degree of precision, and the like all meet relevant demands for ultraviolet absorbent detection in sun block cosmetics at home and abroad. The method is suitable for detection and monitoring of the ultraviolet absorbents in the sun block cosmetics, and has great significance on promoting of import and export trade of cosmetics of our country, ensuring of safety of the cosmetics, and guarantee of human body health.

The present invention relates to a method for one-time simultaneous quantitative determination of multiple antibiotics, comprising: (1) preparing a sample filtrate; (2) setting liquid chromatography conditions and mass spectrometry conditions; (3) using the above liquid chromatography conditions and mass spectrometry conditions to determine For the sample filtrate prepared in step (1), analyze the data to determine the type and content of antibiotics in the analyzed sample. The present invention can simultaneously detect multiple antibiotics in one experiment and quantify the detected antibiotics at the same time. The combination of liquid chromatography-mass spectrometry not only solves the problem of difficult separation of peaks with similar peak times in liquid chromatography, but also overcomes the The error caused by the inconspicuous peak of low ion current intensity in the mass spectrum is eliminated; the invention can be widely used in the detection and quantitative analysis of antibiotics in various samples such as feces, soil, water, food, and the like.

The invention discloses an environmental aerosol direct sampling gathering sample injecting device and a quantitative analysis method. The environmental aerosol direct sampling gathering sample injecting device comprises an environmental aerosol sampling module, an aerosol atomization dissolving generation module, an aerosol condensation growing up module, an aerosol number concentration gathering module and a base body gas removing module. The environmental aerosol sampling module comprises a sampling port, a sampling pump and a connection pipeline, preferably, the sampling pump is a membrane pump; the aerosol atomization dissolving generation module comprises an atomizer, a fog chamber, a drying device and a connection pipeline, the atomizer is used for atomizing a standard solution to generate fog drops, the fog room is used for removing large fog drops and the drying device is used for dissolving the fog drops output from the fog room and removing steam contained in the fog drops. Due to the mode that the environmental aerosol direct sampling gathering sample injecting device serves as an aerosol sample injection system and is used in cooperation with an ICP-MS, quality sample injection rate of an element to be measured in environmental aerosol can be measured quantitatively, and then sample injection volume flow of the environmental aerosol is divided by the quality sample injection rate to acquire the quality volume concentration of the element to be measured.

An apparatus, in particular a dental splint, for the measurement of occlusal forces in case of a terminal occlusion of teeth comprises at least one sensor which is arranged outside of an occlusion region of the apparatus. A system for the diagnosis or treatment of bruxism serves to determine the position or the size of occlusal forces on the apparatus. A method allows exact localization and quantitative determination of occlusal forces on the apparatus.

The invention relates to the analysis of the performance and properties of electrochemical processes, and specifically, to electrolytic solutions and electrode processes. The invention discloses a device and a method for obtaining qualitative and quantitative information for the kinetics of the electrode reactions, the transport processes, the thermodynamic properties of the electrochemical processes taking place in the cell. When a deposition reaction takes place, the device provides also valuable information about the relationship between the current density and deposit properties including but not limited to the deposit color, luster, and other aspects of its appearance. The device disclosed herein typically is comprised of a multiplicity of cathodic or anodic regions where one or more electrochemical reactions take place simultaneously, but at a different rate. From the precisely measured segmental currents one can obtain among other process properties: (1) An accurate relationship between the deposit appearance and the current density. This relationship can be used for process diagnostics, troubleshooting, control of concentrations, pH, and additives and contaminants and for optimizing the operating conditions, including the voltage, current, and circulation rate. (2) Quantitative determination of important process parameters including but not limited to, kinetics (e.g., exchange current density, cathodic and anodic transfer coefficients), transport (e.g. conductivity), and thermodynamics (e.g., standard potential). A particularly attractive application of the process is for the quantitative and qualitative processes of alloys plating and for the determination of the relationship between the current efficiency and the applied current density.

The invention discloses a portable electrostatic detection device and an electrostatic detection method thereof and belongs to the field of detection. A static inductive sensor of the portable electrostatic detection device comprises a shield casing with a signal receiving window; an electrostatic signal receiving surface is arranged inside the casing; a circuit board and a signal transmitting bracket are arranged in the casing; a vibration electrode slice with a vibration source is arranged on the signal transmitting bracket; a covering sheet in parallel to a metallic electrostatic signal receiving surface is arranged at the front end of the vibration electrode slice; the rear end of the vibration electrode slice is fixedly arranged on the signal transmitting bracket or fixedly arranged on the signal transmitting bracket through the vibration source; and an electrical signal processing unit of the portable electrostatic detection device at least comprises a vibration source driving circuit, a signal amplifying circuit, a rectifier circuit and a singlechip circuit. According to the portable electrostatic detection device and the electrostatic detection method thereof disclosed by the invention, the induced electrostatic voltage is converted into alternating voltage by adopting a mechanical interrupt method for an output and subsequent detection/amplifying unit to use, and thus the electrostatic quantitative determination is realized; and in addition, the repeatability of monitored and detected data is favorable and the precision and the stability of the electrostatic detection are improved.

The invention discloses a Fourier transform chip spectrometer based on integrated light technique, comprising an integrated optical waveguide chip having electro-optic modulation function. Light from a light source enters the integrated optical waveguide chip through a fiber transmission coupler, and waveguide light from the chip enters an optical waveguide interferometer through a sensitive window interval. The interferometer outputs signals to a photoelectric detector. A voltage function generator is used for applying voltages varying with time between two modulated electrodes of the optical waveguide interferometer, the photoelectric detector is used for real-time measuring the change of the signal strength of the interferometer varying with the modulated voltage, and a signal processing chip connected with the photoelectric detector and the voltage function generator at same time is used for rapidly processing Fourier transform on the signals of the interferometer to obtain an incident light spectrum. The Fourier transform chip spectrometer provided by the invention can determine visible-infrared light absorbing spectra of solids, liquid, even monomolecular adsorption layers in the sensitive window, and determine fluorescence spectra of fluorescent substances in the sensitive window, with the advantages of strong anti-interference capability, and is suitable for rapid on-site quantitative determination.

The invention discloses a method for measuring coal sample methane adsorbing capacity through low-field nuclear magnetic resonance. According to the method, selected measuring parameters are used for carrying out low-field nuclear magnetic resonance measurement on powder coal samples after methane adsorption balance under set pressure, the nuclear magnetism T2 spectrum of methane in the coal samples is obtained, then signal amplitude integrals of the first spectrum peak (in the range of 0.1-4ms spectrum) on the left of the T2 spectrum are substituted to a hydrogen content index reticle equation of methane under the standard condition built through an experiment, the standard condition size of methane adsorbed by the coal samples is obtained, and therefore the methane adsorbing capacity of unit mass of coal under the set pressure is obtained. According to the method, the scale relation of methane mass and nuclear magnetic resonance 1H nuclear signals is built, the quantitative assay of methane adsorbing capacity under the same temperature and different pressures is achieved, and the novel method can be used for measuring coal methane absorbing capacity in a real-time, home-position and dynamic mode.

The invention provides a method and system for quantitative determination of cooling characteristics of a data center by calculating thermal influence indices. The invention further provides a method and system for providing effective thermal management in a data center using quantitative determination of cooling characteristics of a data center.