5 results about "Competitive immunoassay" patented technology

This application relates to a detection reagent, kit, and method that simultaneously improves sensitivity and broadens the detection range in competitive immunoassay. The reagent includes a detection antibody bound to luminescent microspheres that specifically binds to analyte molecules in the sample, and two sets of labeled antigens containing the same competing antigen and labeled molecules of different molecular weights bound to it. The competing antigen competes with the analyte molecule for binding to the detection antibody, and the affinity of the competing antigen for binding to the detection antibody is higher than that of the analyte molecule for binding to the detection antibody. The technical solution of this application, by using two sets of labeled antigens with different affinities, ensures that the labeled antigens that play a dominant role in samples with varying concentrations of analyte molecules differ. This is suitable for competitive immunoassay of small molecule antigens or haptens in both low-concentration and high-concentration samples, and can broaden the detection range while ensuring high functional sensitivity.

The application discloses a kind of natural product antiviral drug screening method based on hexagon protein and application, belong to active ingredient screening and antiviral drug development field.The method includes: preparation nitrogen-doped mesoporous carbon and silver nanosheet composite solution, drop coating on the surface of pretreated glassy carbon electrode, after drying by activation, hexagon protein incubation, blocking and enzyme label antibody reaction, construct electrochemical sensing electrode;With the electrode, the sample to be screened natural product is incubated, and the current response signal is detected;According to signal change combination inhibition rate formula evaluation antiviral activity.The application takes hexagon protein as recognition target, based on the principle of competitive immunoassay, realizes the specific, high sensitivity detection of antiviral active ingredient in natural product, with the advantages of fast operation, low cost, small sample consumption, high throughput, etc., provides effective technical support for the mining of antiviral active ingredient in natural product such as traditional Chinese medicine.

This invention provides a wash-free, competitive immunoassay method capable of simultaneous multiplex detection. Specifically, this invention utilizes self-developed Raman microspheres (R-Sphere) with non-overlapping Raman signals to conjugate specific antibodies against the target molecule onto the Raman microspheres. The target molecule is then detected through an immunoassay. Raman microsphere-antibody conjugates that do not bind to the target molecule are adsorbed onto the container wall via carboxyl magnetic beads, thereby eliminating background signal interference. This method offers advantages such as wash-free operation, separation-free operation, ease of use, high accuracy, and the ability to simultaneously detect multiple signals.

This invention provides a kit based on a three-phase enzyme-linked immunosorbent assay (ELISA) and its application. The construction of the three-phase ELISA sensor in the kit mainly includes the following steps: first, selecting a hydrophobic substrate, washing and pretreating it; second, immobilizing the coated antigen and oxidase on the substrate. This invention develops a competitive immunoassay method based on a three-phase substrate and applies it to the detection of 5-fluorouracil. This invention utilizes the specific immunoreaction between a collaboratively developed 5-fluorouracil monoclonal antibody and the antigen to quantitatively detect 5-fluorouracil, improving the selectivity for the target analyte. This immunosensor uses inexpensive equipment, is easy to operate, has high detection efficiency, simple sample processing, and exhibits a wide linear range and a high detection upper limit, making it easy to popularize and cost-effective, thus possessing high practical value.

The disclosure generally relates to the detection of symmetrical dimethylarginine (SDMA). More particularly, the disclosure relates to the detection of SDMA using a solid phase. The disclosure provides devices, reagents, kits and methods for detecting symmetrical dimethyl arginine (SDMA) in sample, such as a biological sample from an animal. The method includes detecting the presence or amount of SDMA in the sample by using an immunoassay format, such as a competitive immunoassay. The assay includes the use of antibodies to SDMA that are specific for SDMA and that have less affinity for other arginine derivatives.