13 results about "Competitive immunoassay" patented technology

The present invention provides a quantitative measurement method using competitive immunochromatography, which has not been previously reported. The quantitative measurement method comprises: (1) creating a calibration curve based on the signal intensity of a test line according to the concentration of a standard material by using multiple strips, and calculating the concentration of a target analyte by using the calibration curve; (2) when the concentration of the target analyte increases to a significant level on the calibration curve of the standard material, a labeled signal of a detection ligand on a control line remains unchanged in a case where a detection ligand of a target analyte-detection ligand conjugate binds to and saturates a capture ligand on the control line, making it impossible to estimate the concentration beyond that level; however, by designing a binding site for the capture ligand on the control line to be the target analyte of the target analyte-detection ligand conjugate, a high concentration of the target analyte breaks down the triple conjugate of detection ligand-target analyte-capture ligand, leading to dissociation of the labeled detection ligand and thereby weakening the signal (hook effect); and (3) comparing the signal intensity patterns of the test line and the control line according to the calibration curve of the standard material with the signal intensity pattern of a sample target analyte, and determining how far the target analyte is from the saturation point of the detection ligand. This method is characterized in that, in cases where the target analyte is present in an excessively large amount and exceeds the maximum value of a measurement range (dynamic range) of the calibration curve, the calibration curve using multiple strips and the configurations (test line and control line) of an immunochromatographic device are designed differently to distinguish such cases.

This application relates to a detection reagent, kit, and method that simultaneously improves sensitivity and broadens the detection range in competitive immunoassay. The reagent includes a detection antibody bound to luminescent microspheres that specifically binds to analyte molecules in the sample, and two sets of labeled antigens containing the same competing antigen and labeled molecules of different molecular weights bound to it. The competing antigen competes with the analyte molecule for binding to the detection antibody, and the affinity of the competing antigen for binding to the detection antibody is higher than that of the analyte molecule for binding to the detection antibody. The technical solution of this application, by using two sets of labeled antigens with different affinities, ensures that the labeled antigens that play a dominant role in samples with varying concentrations of analyte molecules differ. This is suitable for competitive immunoassay of small molecule antigens or haptens in both low-concentration and high-concentration samples, and can broaden the detection range while ensuring high functional sensitivity.

The application discloses a kind of natural product antiviral drug screening method based on hexagon protein and application, belong to active ingredient screening and antiviral drug development field.The method includes: preparation nitrogen-doped mesoporous carbon and silver nanosheet composite solution, drop coating on the surface of pretreated glassy carbon electrode, after drying by activation, hexagon protein incubation, blocking and enzyme label antibody reaction, construct electrochemical sensing electrode;With the electrode, the sample to be screened natural product is incubated, and the current response signal is detected;According to signal change combination inhibition rate formula evaluation antiviral activity.The application takes hexagon protein as recognition target, based on the principle of competitive immunoassay, realizes the specific, high sensitivity detection of antiviral active ingredient in natural product, with the advantages of fast operation, low cost, small sample consumption, high throughput, etc., provides effective technical support for the mining of antiviral active ingredient in natural product such as traditional Chinese medicine.

This disclosure relates to anti-immunocomplex antibodies specific for an immunocomplex formed between testosterone and an anti-testosterone primary antibody, as well as a method for the preparation thereof. The disclosure also relates to non-competitive immunoassays and immuno- assay kits for detecting or quantifying testosterone.

Methods of qualifying a subset of target binding biomolecules from a larger set of target binding biomolecules for analysis are disclosed. Interactions between 100 different pairs of target binding biomolecules are identified using a competitive immunoassay comprising target proteins and 200 interaction profiles are generated. Each target binding biomolecule is assigned 300 to a bin representing an epitope family and the identified bins are associated with the identified respective target binding biomolecules in a circular or semi-circular bin chart on a display. Based on the association 400 between the identified bins in the bin chart and the identified respective target binding molecules, a subset of target binding biomolecules is selected 500 for further analysis by selecting one or more target binding biomolecules of one or more bins.

Methods of qualifying a subset of target-binding biomolecules from a larger group of target-binding biomolecules for analysis are disclosed. Competitive immunoassays comprising target proteins are used to identify 100 interactions between pairs of different target-binding biomolecules and produce 200 interaction profiles. Each target-binding biomolecule is assigned 300 into a bin representing the family of epitopes, and the identified bin is associated with the identified respective target-binding biomolecule in a circular or semicircular bin graph on the display. A subset of target-binding biomolecules is selected 500 for further analysis by selecting one or more target-binding biomolecules of one or more bins based on the association 400 between the bins identified in the bin graph and the respective target-binding molecules identified.

The application discloses a fengycin mimotope peptide which can be specifically combined with fengycin antibody, and a sensitive and rapid competitive enzyme-linked immunoassay method can be established by using the phage displaying the mimotope peptide or the mimotope peptide. 50 The IC50 is 0.30 ng / mL, and the LOD is 0.02 ng / mL, and the sensitivity of the method is improved by 60 times compared with the sensitivity of a traditional enzyme-linked immunoassay method based on a hapten; the competitive immunoassay method based on the mimotope peptide has good cross reactivity to other common structural analogues.

The invention relates to an okadaic acid mimic epitope peptide and application. The amino acid sequence of the okadaic acid mimic epitope peptide is shown as SEQ ID NO: 1, 3, 5, 7, 9, 11, 13 or 15. The mimic epitope peptide can be specifically combined with an okadaic acid antibody, and a sensitive and rapid competitive enzyme-linked immunosorbent assay is established by using the phage for displaying the mimic epitope peptide; according to the method, the IC50 is 0.83 ng / mL, the LOD is 0.21 ng / mL, and the detection range is 0.35 to 1.98 ng / mL; the competitive immunoassay method established on the basis of the mimic epitope peptide provided by the invention is more sensitive than the traditional immunoassay method, has good cross reaction to other marine toxins, avoids the use of toxin antigens, and is green and environment-friendly.

This invention provides a wash-free, competitive immunoassay method capable of simultaneous multiplex detection. Specifically, this invention utilizes self-developed Raman microspheres (R-Sphere) with non-overlapping Raman signals to conjugate specific antibodies against the target molecule onto the Raman microspheres. The target molecule is then detected through an immunoassay. Raman microsphere-antibody conjugates that do not bind to the target molecule are adsorbed onto the container wall via carboxyl magnetic beads, thereby eliminating background signal interference. This method offers advantages such as wash-free operation, separation-free operation, ease of use, high accuracy, and the ability to simultaneously detect multiple signals.

This invention provides a kit based on a three-phase enzyme-linked immunosorbent assay (ELISA) and its application. The construction of the three-phase ELISA sensor in the kit mainly includes the following steps: first, selecting a hydrophobic substrate, washing and pretreating it; second, immobilizing the coated antigen and oxidase on the substrate. This invention develops a competitive immunoassay method based on a three-phase substrate and applies it to the detection of 5-fluorouracil. This invention utilizes the specific immunoreaction between a collaboratively developed 5-fluorouracil monoclonal antibody and the antigen to quantitatively detect 5-fluorouracil, improving the selectivity for the target analyte. This immunosensor uses inexpensive equipment, is easy to operate, has high detection efficiency, simple sample processing, and exhibits a wide linear range and a high detection upper limit, making it easy to popularize and cost-effective, thus possessing high practical value.

The invention is applicable to the technical field of in-vitro diagnosis and immunodetection, and provides a 25-hydroxyvitamin D dissociation-neutralization-quantification integrated micro-fluidic chip which comprises a closed shell, a PET (polyethylene terephthalate) film layer and a PMMA (polymethyl methacrylate) substrate layer which are sequentially assembled from top to bottom, the PET film layer is provided with a continuous micro-channel network, the micro-channel network is formed by alternately connecting straight channels and serpentine channels in series, and an alkalization dissociation area, an acidification neutralization area, a delay diffusion area and a detection area are sequentially integrated in the liquid flow direction; according to the invention, the chip is self-driven by capillary force and is combined with the mixing enhancement and driving force supplement effects of the micro-column array, so that efficient dissociation, rapid neutralization and sequential competitive immunodetection of 25-hydroxyvitamin D in a sample are realized, an external pump valve or artificial liquid transfer is not needed, and full-automatic and rapid quantitative detection of'sample input-result output 'is achieved; the problems of complicated operation, difficulty in miniaturization and large detection error in the prior art are solved, and the method is suitable for real-time detection scenes.

The disclosure generally relates to the detection of symmetrical dimethylarginine (SDMA). More particularly, the disclosure relates to the detection of SDMA using a solid phase. The disclosure provides devices, reagents, kits and methods for detecting symmetrical dimethyl arginine (SDMA) in sample, such as a biological sample from an animal. The method includes detecting the presence or amount of SDMA in the sample by using an immunoassay format, such as a competitive immunoassay. The assay includes the use of antibodies to SDMA that are specific for SDMA and that have less affinity for other arginine derivatives.

The invention discloses a three-dimensional gel photonic crystal. The three-dimensional gel photonic crystal is prepared by the following steps: preparing polyvinyl alcohol three-dimensional PCH, acrylated glycocholic acid and an acrylated glycocholic acid antibody, and preparing glycocholic acid antigen-antibody cross-linked PCH through a photopolymerization reaction. The PCH prepared by the invention can be used for detecting glycocholic acid on the basis of a competitive immunoassay principle, free glycocholic acid molecules in a sample and covalently loaded glycocholic acid molecules in hydrogel compete to be combined with covalently loaded glycocholic acid antibodies, so that the initial antigen-antibody crosslinking degree in the hydrogel is reduced to cause the volume change of the hydrogel; further, the structural color change and diffraction wavelength migration of the PCH are caused, so that the visual semi-quantitative and optical quantitative analysis of the glycocholic acid is realized. The PCH-based sensor is simple and convenient to use, can meet the requirement of instant detection, and has the advantages of high sensitivity, strong specificity and reusability, the actual application potential of the sensor is also proved by an artificial serum standard recovery experiment, and the PCH-based sensor has a very good application prospect.