14 results about "Calprotectin" patented technology

The invention provides a calprotectin and slgA combined judgment child digestive state evaluation method and system, and relates to the technical field of data detection.The method comprises the steps that the age of a target child is collected, baseline calprotectin and baseline slgA are configured, and a baseline error coefficient is obtained; collecting body characteristics of the target child and a feeding characteristic sequence in a recent preset time range, configuring a physical sign correction coefficient and a feeding correction coefficient, and correcting to obtain a corrected baseline error coefficient; performing calprotectin and slgA fluctuation prediction according to the feeding characteristic sequence to obtain predicted fluctuation calprotectin and predicted fluctuation slgA, and calculating actual calprotectin and actual slgA; and calculating the fluctuation coefficient of predicted fluctuation calprotectin and predicted fluctuation slgA and the fluctuation coefficient of baseline calprotectin and baseline slgA, calculating to obtain data confidence, labeling, and evaluating the digestive state of children. The technical problem that in the prior art, the accuracy of child digestion state evaluation results is insufficient is solved.

The invention belongs to the technical field of bioengineering, and particularly relates to a preparation method and application of a calprotectin S100A8 / A9 dimer mouse monoclonal antibody, the monoclonal antibody comprises a heavy chain variable region and a light chain variable region; the heavy chain variable region comprises any one of amino acid sequences as shown in SEQ ID NO.22-24; the heavy chain variable region comprises any one of amino acid sequences as shown in SEQ ID NO.22-24; the light chain variable region comprises an amino acid sequence as shown in SEQ ID NO. 28-30. According to the present invention, the S100A8 / A9 heterodimer protein with biological activity and immunostimulation function is prepared, the protein is used to immunize mice, the specific gene engineering antibody of the S100A8 / A9 heterodimer protein is prepared, the antibody pair with high sensitivity is screened, and the calprotectin S100A8 / A9 double-antibody sandwich enzyme-linked immunosorbent assay method is preliminarily developed;

The disclosure relates to a point-of-care test device for diagnosis and detection of bacterial infections and hidden septic processes in samples of bodily fluids of premature and newborns comprising a quantitative or semi-quantitative lateral flow immunoassay unit adapted for parallel detection of proteins of the S100 family and for several detection of increased amounts of S100A12 and calprotectin in blood, serum, plasma, and saliva.

The invention provides a whole blood treatment agent, a kit and application of the whole blood treatment agent in detection of calprotectin, and relates to the technical field of in-vitro detection, the whole blood treatment agent comprises 5-15 mmoL / ml of ammonium chloride or 0.01-0.05 mg / ml of ascorbic acid oxidase, and a solvent is an enzyme diluent. The enzyme diluent is used as a solvent, so that the whole blood treatment agent maintains a stable environment in a sample treatment process, and related enzyme activity is maintained. Ammonium chloride promotes erythrocyte lysis to release hemoglobin; ascorbic acid oxidase can catalyze ascorbic acid to be oxidized into dehydroascorbic acid, and interference factors are reduced. By using the whole blood treatment agent, a tedious pretreatment step on a whole blood sample by using a sample treatment liquid is not needed, the directly collected whole blood sample can be used for subsequent detection after being mixed with the whole blood treatment agent, and the directly collected whole blood sample can be used for subsequent detection, so that the sample treatment is simplified, and the risk of introducing an error due to the sample treatment is reduced; a detection result accords with a clinical diagnosis result, and the accuracy is high.

The invention discloses a detection kit for detecting the content of calprotectin in synovial fluid. The detection kit comprises a detection kit shell and a calprotectin immunochromatography test strip, and the calprotectin immunochromatography test strip is arranged in the detection kit shell; the calprotectin immunochromatography test strip comprises a sample pad, a glass fiber combination pad, a coated nitrocellulose membrane and a water absorption pad which are bonded in sequence, an anti-calprotectin antibody marked by colloidal gold is embedded in the glass fiber combination pad and is used for being combined with calprotectin in a sample to be detected to form a compound; the anti-calprotectin monoclonal antibody is coated on a detection line of the nitrocellulose membrane, and the anti-mouse IgG antibody is coated on a quality control line of the nitrocellulose membrane; the colloidal gold labeled anti-calprotectin antibody is embedded in the glass fiber cushion layer, and the image recognition module is used for collecting a tested test paper image and calculating a relative gray value of a detection line and a quality control line, so that a calprotectin concentration interval is judged.

The application provides an anti-calprotectin monoclonal antibody combination and application thereof. The anti-calprotectin monoclonal antibody combination comprises a first antibody and a second antibody, wherein the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region; the amino acid sequence of the first light chain variable region comprises VL-CDR1-3 shown in SEQ ID NO:1-3; the amino acid sequence of the first heavy chain variable region comprises VH-CDR1-3 shown in SEQ ID NO:4-6; the amino acid sequence of the second light chain variable region comprises VL-CDR1-3 shown in SEQ ID NO:7-9; and the amino acid sequence of the second heavy chain variable region comprises VH-CDR1-3 shown in SEQ ID NO:10-12. The anti-calprotectin monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.

This invention relates to HeartTAC, an antibody protein that targets and degrades calprotectin, and its applications, belonging to the field of biomedical technology. This invention provides HeartTAC, an antibody protein that targets and degrades calprotectin, the amino acid sequence of which is shown in SEQ ID NO. 6 or SEQ ID NO. 4. This invention employs an extracellular targeted protein degradation scheme to construct HeartTAC, an antibody protein that targets and degrades calprotectin. This protein exhibits good targeting specificity to the heart, can degrade calprotectin in the heart, can significantly improve cardiac function, alleviate cardiac fibrosis, and has good safety profile. It opens up an important new direction for the prevention and control of cardiovascular diseases and has promising application prospects.

The invention relates to lactobacillus casei LC189 capable of inhibiting intestinal pathogenic bacteria and application of the lactobacillus casei LC189 capable of inhibiting the intestinal pathogenic bacteria, the lactobacillus casei capable of inhibiting the intestinal pathogenic bacteria is named as a lactobacillus casei LC189 strain, the preservation number is GDMCC No: 63092, and the preservation date is December 26, 2022. A novel lactobacillus casei strain with the effect of inhibiting enteropathogenic bacteria is separated from intestinal mucosa of a healthy sow and is preserved, the strain can inhibit the enteropathogenic bacteria such as escherichia coli, salmonella and staphylococcus aureus, and also can maintain the intestinal health of animals, and the specific expression is as follows: the diarrhea rate of piglets is reduced; the content of ammonia gas and methyl mercaptan in the excrement is reduced, and the pH value of the excrement returns to normal from a weak acid environment; beneficial bacteria are promoted to colonize in intestinal tracts, and the number of the beneficial bacteria is increased; the content of calprotectin in excrement is reduced, the inflammation level in the intestinal tract is reduced, and the intestinal tract health is promoted.

The utility model discloses a special sample extraction test tube for calprotectin, which comprises a tube body with two open ends; the sampling rod is coaxially inserted into the pipe body, and the tail end of the sampling rod extends out of one end of the pipe body and is detachably connected with the end; the tube cover is detachably connected with the other end of the tube body, the tube cover comprises a first cover body and a second cover body, filter cotton is arranged in the first cover body, the second cover body is detachably arranged on the first cover body, and a dripping hole is formed in the top of the first cover body. According to the sample extraction test tube, the first cover body and the second cover body which are detachably arranged as well as the filter cotton arranged on the first cover body are adopted, so that the sample extraction test tube has the characteristics of different detection instruments, medical personnel do not need to prepare different sample extraction devices for different instruments, and the complexity and the cost of operation are reduced.

The utility model provides an excrement calprotectin detection appliance which comprises a transparent sample dilution container, a transparent sealing cover and a detection plate, a sampler is detachably mounted in the transparent sample dilution container through the transparent sealing cover, and the detection plate is rotatably mounted outside the transparent sealing cover; due to the integral arrangement, when in use, diluted sample liquid does not need to drop out of the transparent sample diluting container, can be in contact with the detection card in the detection plate, and can be detected through the detection card, so that the sample liquid can be effectively prevented from leaking, unnecessary pollution and even infection are avoided, and the detection efficiency is improved. Therefore, the whole device is safer and more reliable in the detection process.

An immunosensor device is disclosed for rapid detection of circulating biomarkers associated with thrombosis, including C-reactive protein (CRP), calprotectin, soluble platelet selectin (sP-selectin), and D-dimer. The immunosensors were fabricated using fiber-laser engraving of carbon nanotubes and CO2-laser cutting of microfluidic channels and features, along with the electrochemical deposition of gold nanoparticles to conjugate with biomarker-specific aptamers and antibody. Using unsupervised clustering based on 4-biomarker concentrations, thrombotic events were predicted in 49 of 53 patients. The 4-biomarker combination yielded an area under the receiver operating characteristic curve (AUC) of 0.95, demonstrating high sensitivity and specificity for acute thrombosis prediction compared to the AUC values for individual biomarker: CRP (0.773), calprotectin (0.711), sP-selectin (0.683), and D-dimer (0.739). The immunosensor device with unsupervised clustering provides accurate and efficient methods for predicting thrombosis, guiding personalized medicine.

The invention discloses an anti-panda calprotectin monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of antibodies. According to the invention, a panda calprotectin expression vector is constructed, a high-yield recombinant protein is obtained by using a pichia pastoris expression system, and the high-yield recombinant protein is used as an immunogen to immunize a mouse to obtain the anti-panda calprotectin monoclonal antibody. Furthermore, based on a colloidal gold immunochromatography technology, the first non-invasive kit for rapidly detecting calprotectin in a panda excrement sample is established, and the kit is suitable for non-invasive inflammation monitoring of pandas in scenes such as natural reserves, zoos and field scientific investigation; the method is of great significance in monitoring and screening intestinal diseases of wild and captive pandas.

The invention provides an anti-calprotectin monoclonal antibody combination and application thereof.The anti-calprotectin monoclonal antibody combination comprises a first antibody and a second antibody, the first antibody comprises a first light chain variable region and a first heavy chain variable region, and the second antibody comprises a second light chain variable region and a second heavy chain variable region; wherein the amino acid sequence of the first light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 1-3; the amino acid sequence of the first heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 4-6; the amino acid sequence of the second light chain variable region comprises VL-CDR1-3 as shown in SEQ ID NO: 7-9; and the amino acid sequence of the second heavy chain variable region comprises VH-CDR1-3 as shown in SEQ ID NO: 10-12. The anti-calprotectin monoclonal antibody combination can improve the sensitivity and accuracy of in-vitro detection.