The invention relates to a
colloid gold duplex test strip for detecting
fecal occult blood and a preparation method thereof. The
colloid gold duplex test strip is composed of a bottom lining, a sample pad, a marker pad, a
coating film and bibulous paper, wherein the sample pad, the marker pad, the
coating film and the bibulous paper are sequentially adhered to the bottom lining in a
lap joint mode. The marker pad comprises a
colloid gold marked mouse against human Hb
monoclonal antibody Hb1 and a mouse against human Tf
monoclonal antibody Tf1, the concentration of the mouse against human Hb
monoclonal antibody Hb1 is 5-20 Mu g / mL, and the concentration of the mouse against human Tf
monoclonal antibody Tf1 is 5-20 Mu g / mL. According to the colloid gold duplex test strip,
hemoglobin and
transferrin in faeces are detected synchronously, the specificity is high, the sensitivity is high (the
detection limit of Hb is 200 ng / ml, and the
detection limit of Tf is 40 ng / ml respectively), the positive
detection rate of the
digestive tract hemorrhagic
disease is improved remarkably, the
asymptomatic, slight and continuous
alimentary tract hemorrhage which cannot be seen by naked eyes and a
microscope can be accurately detected, and the colloid gold duplex test strip can avoid the interference of
medicine, such as animal blood and so on.