A test strip, a detecting device, and a detecting method are disclosed. The test strip includes a first specimen path, a first electrode set, a second specimen path, a second electrode set, and a reaction reagent. When the specimen contacts the first electrode set and the second electrode set, a first pulse signal and a second pulse signal are generated for obtaining a flow time of the specimen. When the specimen contacts the reaction reagent, the analyte concentration of the specimen can be obtained, and the concentration of the analyte can be corrected by the flow time.