The application relates to the technical field of
germplasm resource protection, and particularly relates to a method for recovering
Acer truncatum Bunge seeds and application. The
recovery method comprises the following steps: using a thawing device to gradient-thaw the ultra-low-temperature-preserved
Acer truncatum Bunge seeds, to obtain thawed seeds; the
transport time of the gradient-thawing is less than or equal to 5 min; using a composite washing liquid to wash the thawed seeds, to obtain sterile seeds; using a repair culture medium to culture the sterile seeds in a dark environment, to obtain primary
recovery seeds; using a basic culture medium to culture the primary
recovery seeds, to complete the recovery of the
Acer truncatum Bunge seeds; the thawing device comprises a base, a thawing part, a transport part and a fixing frame; the thawing part comprises a thawing partition plate, a first thawing groove, a second thawing groove, a constant-temperature tube, an isolation plate and the fixing frame; the transport part comprises a screw rod, a threaded sliding block, an extension rod, a transport fixing rod and a transport connecting block. The
recovery method realizes a relatively high recovery
survival rate of the Acer truncatum Bunge seeds by means of the precise
temperature control core of the gradient thawing, in combination with the device structure design, the matching
processing steps and the repair strategy.