521results about "Dead plant preservation" patented technology

The present invention is in the field of agricultural pathogen control. Methods and compositions are described for controlling plant diseases caused by pathogens, including fungi and oomycete phytopathogens. Chemicals were screened for activity as autophagy inhibitors. The screening method used a bioluminescence resonance energy transfer (BRET)-based high-throughput screening strategy to identify compounds that inhibit fungal ATG4 cysteine protease-mediated cleavage of ATG8 for autophagosome formation. Ebselen and ebselen analogs were identified as inhibitors of fungal pathogens ATG4-mediated ATG8 processing. Identified chemicals inhibit spore germination, hyphal development, and appressorium formation. The autophagy inhibitors significantly reduced the virulence of pathogens in their corresponding plant hosts. The invention also provides for compositions comprising the same, methods of making the same, and methods of controlling plant disease.

The invention belongs to the technical field of flower postharvest treatment, and discloses a processing method for rapid dyeing and fresh-keeping cooperative treatment of cut babysbreath and a special preparation. The method comprises the following steps: (1) preparing a dyeing-fresh-keeping composite special preparation formula; (2) a dyeing-fresh-keeping process: S1, early-stage preparation; s2, flower material treatment; s3, dyeing treatment; s4, observing and adjusting; and (3) post-treatment. The problems that the cut babysbreath is not full in color, easy to fall off, shortened in flowering phase and the like after being dyed are solved through a low-molecular dye composite permeation enhancing system, an ethylene signal whole-course inhibition and conduit protection technology and the like, the operation is simple and easy to implement, the cut babysbreath is rapidly dyed within 30 minutes, petals are uniformly dyed, the color is stable, and the quality of the cut babysbreath is improved. Meanwhile, the freshness and attractiveness of the flowers are guaranteed, the vase life of the cut flowers is not affected, and the flowering phase reaches 20-24 days. The fresh-keeping effect of the babysbreath cut flowers after dyeing can be remarkably improved, and the method has high market application value and commercial potential.

A clathrate of 1-methylcyclopropene with α-cyclodextrin, obtained as a solid particulate product, is modified by comminuting, classifying, or both to obtain a modified particulate. When subjected to identical atmospheric disgorgement conditions of humidity and temperature, identical masses of the modified and unmodified particulates exhibit different rates of 1-methylcyclopropene disgorgement. Specifically, we have found that a smaller mean particle size is inversely related to a greater rate of 1-methylcyclopropene release.

The invention provides an exosome from rice as well as a preparation method and application thereof. The exosome has a double-layer membrane spherical structure, and the particle size range is 20-1000nm. The exosome is obtained from rice calluses, and has the characteristics of high biological safety, high stability and sustainable mass production. And tests prove that the exosome has the functions of resisting aging, promoting cell regeneration and tissue repair, protecting and the like, and also can remarkably improve the cell absorption efficiency and the delivery efficiency of active ingredients such as peptide cells.

Tea tree cultivation, and specifically relates to use of sodium selenite in prevention and / or alleviation tea tree sunburn in the prevention and / or alleviation tea tree sunburn. Specifically, the sodium selenite is applied to the tea tree to prevent and / or alleviate sunburn of the tea tree, avoid damage(s) caused by high temperature and drought weather to the tea tree, and reduce losses caused by the sunburn. The method for preventing and / or alleviating tea tree sunburn using sodium selenite is simple to operate and highly efficient. Compared with the existing methods of shading, reasonable dense planting, soil cultivation, drought prevention, and timely irrigation, the method in the present disclosure shows lower economic and labor costs, such that tea farmers can achieve a higher profit.

The present invention is directed to a method for combining dehydrofreezing and medium to far spectrum infrared radiation to preserve biological material. IR transfers energy and penetrates food products, thereby removing naturally present moisture. The present invention provides a novel method of combining pH treatment, infrared radiation, freezing, and vacuum-sealing for preserving biological material. Biological materials are preserved through either a pH treatment or with heat to remove harmful pathogens, followed by processing using far spectrum infrared radiation which may be powered by either gas or electric means. The processed materials undergo a freezing process which may be done using Individual Quick Freezing or blast freezing methods The frozen processed material is then packaged via vacuum sealing.

According to the technical scheme, the copper-doped lasting antibacterial fresh-keeping material comprises a base body, an antibacterial nanofiber and copper ion loaded antibacterial hydrogel layer and a surface coating, the antibacterial nanofiber and copper ion loaded antibacterial hydrogel layer comprises antibacterial nanofibers and copper ion loaded antibacterial hydrogel, and the surface coating is coated with the antibacterial nanofibers and the copper ion loaded antibacterial hydrogel. The surface coating comprises microcapsules for contactable food products; the preparation method comprises the following steps: preparation of the copper-tannic acid coordination compound, preparation of the antibacterial nanofiber layer, preparation of the copper ion loaded antibacterial hydrogel, preparation of the substrate, interlayer compounding process, preparation of the sustained-release microcapsule coating, crosslinking and post-treatment. The humidity-responsive on-demand release of copper ions of the fresh-keeping material is realized, the antibacterial and fresh-keeping effects are good, the safety is high, and the environment-friendly effect is realized.

The invention discloses a plant cell vesicle efficient extraction method based on the synergistic effect of three-dimensional gradient micro vortex and sound wave fluidization and application of the plant cell vesicle efficient extraction method. According to the plant cell vesicle extraction method provided by the invention, high-yield, high-purity and high-activity extraction of plant cell vesicles is realized through an active enzymolysis-acoustic cracking-three-dimensional gradient separation full-chain process, and the plant cell vesicle extraction method can be used for large-scale production. The method overcomes the defects of low yield and purity, chemical pollution, low extraction efficiency, uncontrollable vesicle size, easily damaged structure, long time and the like of the traditional extraction method, and can be used for producing and preparing more cosmetics and medicines containing plant cell vesicles on a large scale. The method systematically solves the global problem of extraction and application of the plant vesicles through multidisciplinary cross innovation (acoustics, fluid mechanics and molecular bionics), is high in extraction efficiency, good in activity effect and comprehensive and advanced in technical parameters, and has important significance on extraction and development of the plant cell vesicles.

The invention discloses an abelmoschus manihot exosome extraction process, and relates to the field of extraction and purification in biotechnology, and the abelmoschus manihot exosome extraction process comprises the following steps: (1) providing a fresh flower material of abelmoschus manihot, and carrying out mechanical cleaning and surface impurity removal; and (2) homogenizing the cleaned fresh flowers of the abelmoschus manihot for 5-30 minutes by a high-shear homogenizer under the condition that the rotating speed is 8000-15000rpm to obtain homogenate. The method is specially designed for the physiological characteristics of the fresh flowers of the abelmoschus manihot, and through the core principle of'rapid treatment and low-temperature operation ', the activity loss of the exosomes in the extraction process is reduced to the greatest extent, so that the yield of the exosomes is increased, and the yield of the abelmoschus manihot is increased. The method ensures the integrity of the natural structure and function, creatively adopts a process route of combining continuous flow centrifugation and multistage tangential flow ultrafiltration, realizes continuous and efficient separation and purification of exosomes from a large number of plant raw materials, solves the industrial bottleneck that a traditional ultracentrifugation method cannot realize large-scale production, and is suitable for industrial production. And the treatment capacity can easily reach 5-15 tons of fresh flowers per day.

They are prepared by a high energy method (e.g. by sonication) from: an oily solution of edible oils (e.g. high-oleic sunflower oil) as solvent, with melatonin (e.g. 100 μM) and edible fat-soluble antioxidant agents (e.g. 100 μM α-tocopherol), and an aqueous solution of edible water-soluble stabilizing agents (e.g. dextrin 1% w / v). These formulations, applied externally (e.g. by spraying) are useful for preharvest application and / or postharvest preservation of fruits or vegetables, particularly of stone fruits, and specifically of sweet cherries. In two varieties of sweet cherries and two varieties of plums, preservations with the formulations are significantly better than those reached with aqueous solutions of melatonin, or with a commercial formulation, or in the absence of treatment.

Compositions comprising complexes of 1-methylcyclopropene and α-cyclodextrin, and articles including the composition, are provided. Methods for using the compositions and articles in delaying the maturation of fruits, vegetables, and plants are also provided.

A system for the hypothermic transport of biological samples, such as tissues, organs, or body fluids. The system includes a self-purging preservation apparatus to suspend a sample in preservation fluid and perfuse a tissue with preservation fluid. The self-purging preservation apparatus is placed in an insulated transport container having a cooling medium. When assembled, the system allows for transport of biological samples for extended periods of time at a stable temperature.

Solutions and methods for forming an exogenous flexible film on a plant and plants including the exogenous flexible film. The solutions include a solvent, a film forming matrix component, a hydrophobic barrier component, a plasticizing component, and a film enhancing component. The methods include spraying the solution on the plant. The methods also include evaporating at least a fraction of the solvent from the solution to form the exogenous flexible film. The plant includes fruit, and the spraying includes spraying at least 45 days prior to harvesting the fruit. The plants include an exogenous flexible film formed from the solutions and / or by the methods.

The invention provides application of a gastrodia elata extract in preparation of a flower preservative, and belongs to the technical field of flower preservation. According to the application of the gastrodia elata extract in preparation of the flower preservative provided by the invention, more gastrodia elata products are expected to be developed by utilizing low-quality tubers, tuber peels, overground pedicels and other parts of gastrodia elata, and comprehensive utilization of gastrodia elata is realized; the gastrodia elata extracting solution is extremely good in antibacterial effect, is rich in various nutrients required by the fresh cut flowers, and has great application potential as a fresh cut flower preservative; compared with a chemical preservative, the gastrodia elata extracting solution is green, environment-friendly and healthy, has extremely low risk to a human body, and is an environment-friendly and sustainable preservative.

The invention provides a preparation method and application of centella asiatica stem cells for high-yield madecassoside, and belongs to the technical field of stem cells. The preparation method comprises the steps of disinfection, induction, proliferation, culture of centella asiatica stem cells with high madecassoside yield and the like, and has the unexpected technical effects that the pollution rate is low, the induction rate is high, the proliferation multiple is high, and the madecassoside content in the obtained centella asiatica stem cells with high madecassoside yield is high. The product is beneficial to industrial production and can be used as a skin care component in a product with anti-inflammatory, soothing and other effects.

To provide a method for producing dried flower that replaces tissue water of a cut flower with polyethylene glycol in a relatively short time before the cut flower begins to wither, thereby keeping the cut flower in a fresh state for a long period.SOLUTION: A method for producing dried flower, comprises a step of immersing a stem portion of a cut flower in a container filled with an aqueous polyethylene glycol solution, irradiating with ultrasound, and infiltrating the polyethylene glycol solution into the cut flower, wherein the ultrasound irradiation is performed in a direction substantially perpendicular from the stem side with respect to the longitudinal direction of the cut flower.SELECTED DRAWING: None

A tube pressing device, a cryopreservation tube picking assembly and a biological sample cryopreservation device.The cryopreservation tube picking device comprises a main body and a tube taking piece arranged at the lower end of the main body, the tube taking piece is used for picking a picked cryopreservation tube from a cryopreservation box, the cryopreservation box is used for containing a plurality of cryopreservation tubes, the tube pressing device comprises a connecting piece, one end of the connecting piece is connected with the main body, and the other end of the connecting piece is connected with the cryopreservation tube picking assembly; the other end of the connector is located at the lower end of the body; and the tube pressing plate is connected with the other end of the connecting piece, a tube picking hole corresponding to the position of the tube taking piece is formed in the tube pressing plate, the tube picking hole is used for picking the picked cryopreservation tubes by the tube taking piece, and the tube pressing plate is used for pressing at least part of the cryopreservation tubes in the cryopreservation box when the picked cryopreservation tubes are picked by the tube taking piece. In the process of picking out the picked cryopreservation tubes, at least part of the cryopreservation tubes of the picked cryopreservation tube accessory can be pressed by the tube pressing plate, so that the cryopreservation tubes are prevented from being picked up.

The invention discloses a method for efficiently extracting rose exosomes, which comprises the following steps: (1) pretreatment of rose petals: refrigerating and pre-cooling fresh rose petals, and then alternately irradiating with red light and blue light; (2) preparing rose homogenate: mixing rose petals with an antioxidant solvent, and homogenizing to obtain the rose homogenate; (3) exosome extraction: performing ultrasonic treatment on the rose homogenate; (4) exosome purification: filtering the rose homogenate to remove residues so as to obtain a rose exosome purified solution; and (5) exosome preservation: adding a solid membrane component into the exosome purified liquid, and freeze-drying to obtain exosome powder. Red light and blue light are alternately irradiated to promote release of the exosome in cells, and the composite antioxidant reagent is adopted to protect the exosome, so that the extraction efficiency and the integrity of active ingredients in the exosome are remarkably improved, the extraction cost is reduced, and powerful support is provided for large-scale industrial production.

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

The present invention relates to methods of treating botanical material to incorporate an aerosol-former material. In particular, the methods are suitable for treating leaf material, such as tobacco leaf. The treated material is well suited to being incorporated into aerosol generating material for use in aerosol provision systems and the like.

The invention relates to a fresh flower preservation method which sequentially comprises the following steps: manually poking grape seeds, cleaning and drying, grinding powder to obtain grape seed powder, adding ethanol in a ratio of 1: 10, adopting a microwave soaking method to obtain a preservation solution, diluting by 50 times, and uniformly spraying on leaves by adopting a spraying method. According to the fresh flower preservation method provided by the invention, the storage time of the petals of the fresh flowers can be prolonged, the colors of the petals cannot be obviously changed after the petals are placed for a long time, meanwhile, the period of water loss and withering is also greatly prolonged, and the sales range of the fresh flowers is expanded.

The camellia cut flower comprises a bionic pedicel, a sleeve is detachably connected to the upper end of the bionic pedicel, a liquid storage bottle is arranged at the upper end of the sleeve, a bottle cap is detachably connected to the upper end of the liquid storage bottle, a bionic stylus is arranged in the middle of the upper end of the bottle cap, and a plurality of bionic calyxes are arranged on the peripheral side of the upper end of the bottle cap. A siphon hole communicated with the liquid storage bottle is formed in the side, on the bionic stylus, of the bottle cap, a siphon cotton thread is arranged in the siphon hole, and the camellia is tightly buckled on the bionic stylus after sepal and pistil parts are removed. By arranging the bionic styles and the bionic calyxes, the parts, except sepals and pistils, of the camellia flowers are fixed, and the problem that the flowers are prone to falling in the picking and transporting process when the camellia flowers serve as fresh cut flowers is solved. And through the liquid storage bottle and the siphon cotton thread, the problem that the tea flower vase is easy to dehydrate is solved, and the vase life of the camellia is obviously prolonged. Wherein the bionic pedicel overcomes the problem that the pedicel of camellia is short but not straight.

The utility model provides efficient ecological preserved flower processing equipment, and belongs to the technical field of ecological plant culture equipment. The efficient ecological preserved flower processing equipment comprises a soaking tank, a pressure tank and an air compressor, the soaking tank comprises a tank body, the tank body is provided with an inner cavity used for storing liquid medicine, and the soaking tank further comprises a top cover and a driving mechanism which drives the top cover to move and enables the top cover to close or open the inner cavity. According to the efficient ecological preserved flower processing equipment, plants are completely soaked in the liquid medicine, so that the plants can be in comprehensive contact with and absorb a treating agent, the processing period of the ecological preserved flowers can be shortened, and the processing efficiency is well improved.

A wood preservative composition and method for preserving wood by contacting wood with said composition comprising a polyurethane polymer, non-aqueous solvents, and a wood preservative active.

The invention relates to the technical field of cut flower postharvest preservation, and discloses a preservation pre-treatment solution for maintaining moisture-nutrition-microecological balance in a storage period of babysbreath cut flowers and a synergistic application method thereof. The pre-treatment liquid is prepared from the following components in percentage by weight / volume: 15 to 30 g / L of cane sugar, 15 to 30 g / L of trehalose, 100 to 200 mg / L of 8-hydroxyquinoline citrate, 50 to 150 mg / L of citric acid, 50 to 100 mg / L of carboxymethyl chitosan, 0.3 to 1.0 g / L of lysozyme-chitosan compound, 5 to 15 mg / L of nano silver-loaded silicon dioxide, 0.1 to 1.0 g / L of manganese citrate and 0.5 to 1.0 g / L of calcium nitrate. And 5 to 10 mL / L of glycerol and 0.5 to 5.0 mL / L of Tween-20 in percentage by volume. Through component collaborative design-process precise control-package matching innovation, the bottleneck of double loss of water and nutrition in the storage period is broken through, the pain points of no flowering and gray mold outbreak after storage are solved, a whole-process nursing scheme is provided for long-distance and short-distance transportation and storage of the babysbreath cut flowers, the commodity rate of the cut flowers is remarkably increased, and the economic benefit is increased. The method is suitable for flower harvesting bases, cold chain transportation and wholesale markets, and is especially suitable for air transportation exits.

To provide a freshness retaining device capable of cooling and taking ethylene countermeasures without using electrical power to retain freshness of at least either fruits and vegetables or flowering plants.SOLUTION: A dry ice container 3 is provided inside a storage container 2. The dry ice container 3 accommodates dry ice 12, and transfers coldness of the dry ice 12 into the storage container 2. A carbon dioxide supply part 4 is provided that supplies carbon dioxide, generated by sublimation of the dry ice 12 in the dry ice container 3, into the storage container 2.SELECTED DRAWING: Figure 1

The invention relates to the technical field of forestry resource protection. The invention provides an ancient castanopsis hystrix tree resource collection, storage and evaluation method, and the method can systematically collect ancient castanopsis hystrix tree resources, and lays a solid data foundation for subsequent protection and research work. The collected ancient castanopsis hystrix tree resources are preserved properly, so that the survival rate of subsequent grafting or planting is guaranteed to the greatest extent, and the original ecological and biological characteristics of the ancient trees are maintained; the castanopsis hystrix ancient tree resources are objectively and accurately evaluated from multiple dimensions, and a powerful decision basis is provided for reasonable development, sustainable utilization and scientific research of the castanopsis hystrix ancient tree resources.

The utility model relates to a test device, in particular to a wheat primary color specimen preparation device. The device comprises a bottom plate, a vertical plate, a connecting plate, a horizontal position adjusting assembly, a vertical position adjusting assembly, a cleaning barrel and a soaking barrel, the bottom plate is perpendicular to the vertical plate, and the horizontal position adjusting assembly is arranged above the bottom plate and connected with the vertical plate through the connecting plate; the vertical position adjusting assembly vertically penetrates through the horizontal position adjusting assembly; and a cleaning barrel and a soaking barrel are arranged on the upper surface of the bottom plate.

The invention belongs to the technical field of plant germplasm resource preservation, and provides a special protective agent for pollen integrated treatment of Chinese honey locust hybrid flowers, a kit and an integrated treatment method thereof. The protective agent is prepared from 8%-12% w / v of trehalose, 0.05%-0.08% v / v of Triton X-100 and a phosphate buffer solution with the pH value of 7.0. The integrated treatment method comprises the following steps: performing stamen morphology primary screening and separation on the Chinese honey locust flowers at low temperature; grinding in an ice bath by using a protective agent, filtering, centrifuging and enriching fertile pollen; quickly evaluating the activity of the split-flow part suspension by using an MTT method; carrying out trehalose-tert-butyl alcohol gradient dehydration and low-temperature vacuum drying on the residual part to obtain dried pollen, and storing for a long time; and treating with rehydration liquid before use. The extraction damage is reduced to the maximum extent; tert-butyl alcohol and trehalose synergistically dehydrate and dry, so that the pollen safely enters a vitrification state, and long-term stable preservation is realized; the repeatability is high, standardized operation is achieved, and a reliable tool is provided for gleditsia sinensis germplasm resource preservation and breeding.

The utility model discloses a specimen making device for primary school science teaching, and relates to the technical field of specimen making devices, the specimen making device comprises an L-shaped bottom plate, an overturning groove and a side inverted L-shaped top plate, the top of the side inverted L-shaped top plate is provided with a pressing assembly extending to the lower part of the side inverted L-shaped top plate, and the pressing assembly is used for pressing a plant specimen; the pressing assembly comprises a pressing unit and a limiting unit; the pressing unit is used for pressing the multiple layers of plant specimens; and the limiting unit is used for limiting and locking the overturning of the side inverted L-shaped top plate. By arranging the L-shaped bottom plate, the side inverted L-shaped top plate and the pressing assembly, plant specimens can be pressed conveniently, when absorbent paper needs to be replaced, only the knob needs to be screwed to enable the pressing plate to relieve extrusion on the plant specimens, then the side inverted L-shaped top plate and the pressing assembly are turned over and opened integrally, operation is easy and convenient, and operation of pupils is facilitated.