The invention belongs to the technical field of food with homology of medicine and food, and particularly relates to a preparation method of rose ferment. The method comprises the steps of separating petals, sieving, protecting color, carrying out superfine grinding, carrying out fermentation pretreatment, planting bacteria for the first time, planting bacteria for the second time, planting bacteria for the third time, finely filtering and feeding collagen to obtain rose ferment liquid. According to the method, probiotic strains which are good for the intestinal tract of a human body are selected for use, and the fermentation temperature is strictly controlled, so that the loss of effective components in roses can be effectively reduced, and the activity of the rose ferment is maintained to the utmost extent; the dominant bacteria are implanted for three times, so that the growth of other bacteria can be effectively inhibited, and the product quality is stable; lactobacillus acidophilus, lactobacillus thermophilus and lactobacillus plantarum are selected, have symbiosis performance and can be planted in the intestinal tract after being fermented in a mixed way; furthermore, the comprehensive activity of ferment products can be better improved. The prepared rose ferment has a certain prevention function for chronic diseases such as heart disease and liver disease, and has the effects of soothing the liver and activating the spleen. After being eaten for a long time, the rose ferment also has the functions of smoothing the skin, whitening the skin and beautifying the face.