39 results about "Pedicel" patented technology

This invention provides a method for storing mango seeds, belonging to the field of seed preservation technology. The method involves cutting off mango fruits with embryos, along with their pedicels and branches, 77-84 days after fruit set. A mixture of pyraclostrobin, lambda-cyhalothrin, and chlorothalonil is sprayed on the fruit, pedicels, and branches. After drying, styrene-butadiene latex is applied to the pedicels and branches. Once the latex dries, a mixture of guanidine-copper acetate and benzoyl-pyraclostrobin is sprayed on the fruit, pedicels, and branches again. After drying, the mango fruits with petioles and branches are placed in a sealed box and stored at 10-14°C. The mango variety used is the Golden Phoenix mango. This invention increases the storage period of Golden Phoenix mango seeds from 1.5 months to 5 months, which is of great significance for the genomics and cell biology research of Golden Phoenix mangoes.

This invention provides a biomimetic bud-removing claw for kiwifruit and its control method, comprising an inverted T-shaped frame, a clamping claw, a left suction claw, a right suction claw, an image detection system, and a control system. This biomimetic bud-removing claw dynamically monitors the pose of buds and pedicels using deep learning and stereoscopic vision. The clamping claw grips the main pedicel, while the biomimetic threads of the suction claw's fingertips grip the lateral buds. Based on pressure feedback, the rotation or contraction amplitude of the suction claw is adjusted to remove the lateral buds, achieving an effect similar to human hand-eye coordination during harvesting. This protects the main bud and pedicel from damage while achieving precise removal of lateral buds. The control method employs a deep fusion model of YOLOv8 and Mask RCNN to extract the main pedicel, left and right lateral buds, and their angle and orientation features, enabling the detection of kiwifruit pedicels and buds, and controlling the clamping and suction claws. Its effect is equivalent to a human's simultaneous observation, clamping, and harvesting control function.

A new cultivar of Agapanthus plant named ‘FO21WM111’ that is characterized by its short leaves, its high quantity of flowers per umbel that are white in color, its floriferous habit with flowering commencing early in the season, and its relatively short pedicels.

The invention provides a pedicel and inflorescence extract ZTM100 of Gastrodia elata Blume, a preparation method of the pedicel and inflorescence extract ZTM100 and application of the pedicel and inflorescence extract ZTM100 in the technical field of cosmetics and medicines. Belongs to the technical field of cosmetics and medicines. The ZTM100 (100 [mu] g / mL) provided by the invention can significantly improve the expression quantity of human dermal fibroblast elastin gene Elastin under UVA radiation, and does not show obvious cytotoxicity to human fibroblasts in a concentration range of 800 [mu] g / mL. The ZTM100 can be used for preparing medicines or cosmetics for resisting skin aging caused by UVA, reducing scar formation, repairing skin injury and repairing wounds.

This invention belongs to the field of plant cultivation technology, specifically relating to a composite substrate for Phalaenopsis orchid cultivation and its application, comprising: sphagnum moss and fermented material, wherein the fermented material includes at least one of wolfberry branch fermented material and Caragana korshinskii branch fermented material; the volume ratio of the sphagnum moss to the fermented material is (5-7):(4-6). Experiments show that using the composite substrate of this application to cultivate Phalaenopsis orchids can promote healthy plant growth. Compared with pure sphagnum moss cultivation, the plant height of Phalaenopsis orchids increased by 15.03%, leaf width increased by 25.42%, and the number of flowers increased by 64.7%; and there was no significant difference in flower diameter, internode length, stem thickness, leaf length, leaf width, pedicel length, and pedicel thickness compared with sphagnum moss cultivation. At the same time, it greatly reduces the proportion of sphagnum moss used and the planting cost, and can realize the resource utilization of regional agricultural and forestry waste, promoting the development of ecological circular agriculture.

The invention relates to a method for regulating and controlling a flower type by silencing a peony organ gene based on a transient expression system, which belongs to a peony flower type regulation and control method and comprises the following steps of: designing a specific primer aiming at a target gene PsuAG, obtaining a target fragment through PCR (Polymerase Chain Reaction) amplification, and inserting the target fragment into a transient expression vector to construct a gene silencing fusion expression vector; introducing the gene silencing fusion expression vector into an agrobacterium competent cell, preparing an infection solution to infect a peony plant, and injecting the infection solution into a pedicel by using an injector when a flower bud of the peony plant develops to an initial stage of a large wind chime period; when the flower buds grow to the initial stage of the round peach stage, injecting the infection liquid into pedicels and sepal bases; after each time of injection, weak light culture is carried out under a shading condition. The efficient silencing of the key gene PsuAG for regulating stamen petals is realized, and the double petals rate of the peony flower type is improved. The method does not need to depend on a stable genetic transformation system, operation is directly carried out on floral organs of a complete plant, and the gene function verification period is remarkably shortened.

The invention provides a short-pedicel aconite root extract as well as an extraction process and application thereof, and belongs to the technical field of medicinal plant processing. According to the technical scheme provided by the invention, active ingredients in the short-pedicel aconite root can be effectively enriched, the purity and the quality stability of the extract are remarkably improved, meanwhile, impurities are effectively removed through optimized process steps, and the production process is simplified; the obtained extract has clear component characteristics and remarkable biological activity, lays a solid foundation for developing related medicines, and has important application value in the aspects of modernization and quality control of traditional Chinese medicines.

The invention discloses a pharmaceutical composition containing sodium calcium edetate and dimercaptosuccinic acid as well as a preparation method and application of the pharmaceutical composition, and relates to the technical field of biological medicines. The pharmaceutical composition comprises the following components: penthorum chinense pursh extract, tuber fleeceflower stem extract, anchusa, muskmelon pedicel, pomegranate root bark, dimercaptosuccinic acid and sodium calcium edetate. According to the invention, by carefully selecting each component and dosage and selecting proper traditional Chinese medicines and western medicines for mutual matching, the research finds that the pharmaceutical composition can effectively inhibit the growth of melanoma cells, is safe and free of side effects to human bodies after being used, and is suitable for large-area popularization and application.

The utility model discloses a kind of fruit stalk separating devices, including bottom plate, the top of bottom plate is provided with separating mechanism, the separating mechanism includes rotating shaft, the lower end of rotating shaft is rotatably connected with bottom plate, and the rotating shaft is perpendicular to the bottom plate, the upper surface of bottom plate is fixedly connected with outside frame, the top of outside frame is fixedly connected with fixed plate, the upper end of rotating shaft is through the inside of fixed plate, and the rotating shaft is rotatably connected with the fixed plate, the lower end of rotating shaft is fixedly connected with two fixed rods, the upper side of fixed rod is fixedly connected with separating rod, the bottom of outside frame is provided with discharge gate. The utility model can quickly separate fruit body and stalk of Amomum villosum by separating mechanism, increase the separation efficiency of fruit body and stalk of Amomum villosum, reduce the labor intensity of staff.

The utility model provides a Paeonia ostii petal picking device, relates to the field of deep processing of Paeonia ostii, and is mainly used for solving the problem of high cost of manual picking of Paeonia ostii petals. The paeonia ostii petal picking device comprises a box body used for containing paeonia ostii petals, a hollow cone is arranged in the box body, and the cone head of the hollow cone is arranged upwards; the conical head part is provided with a circular cutting edge, and an insertion tube for inserting a peonies stem is arranged in the conical head part; a driving roller and a floating roller are arranged in the hollow cone, and the driving roller and the floating roller are oppositely arranged and used for clamping pedicels of peonies and pulling the pedicels and stamens of the peonies out of the insertion pipe downwards. The petal picking device is easy to operate, petals can be automatically picked from the roots only by inserting the picked peonies into the insertion pipes, completeness of the petals can be guaranteed, time and labor are saved, and the petal picking cost is greatly reduced. The peony picking device is convenient to use and can be used in cooperation with peony picking.

A harvesting device includes: an upper harvest ring that an object grown on a plant is to be inserted into; a lower harvest ring that is disposed below the upper harvest ring and that the object is to be inserted into; a first drive mechanism that moves at least one of the upper harvest ring and the lower harvest ring; and a controller. The controller determines one of a first harvest operation and a second harvest operation, and controls the first drive mechanism, based on the one of the first harvest operation and the second harvest operation.

The utility model discloses a chrysanthemum picking device, and relates to the technical field of chrysanthemum picking. A chrysanthemum picking device comprises a movable frame, a carding plate is fixedly connected to the top end of the movable frame, a shielding frame is fixedly connected to the top end of the carding plate, pushing handles which are longitudinally symmetrical are fixedly connected to the movable frame, and the chrysanthemum picking device further comprises guide rails which are distributed at equal intervals and fixedly connected to the bottom end of the carding plate. Longitudinal symmetrical sliding rods are connected into the guide rails in a sliding mode, and cutting blades are fixedly connected to the sliding rods. The movable frame drives the combing plate to move rightwards, miscellaneous branches and chrysanthemums are combed through comb teeth of the combing plate, flowers are clamped between gaps of every two adjacent comb teeth, the cutting blades move oppositely, pedicels of the chrysanthemums can be cut below the comb teeth, the chrysanthemums are separated from plants, and the blocking frame can prevent the chrysanthemums on the combing plate from falling off. The damage to plants can be reduced, and the integrity of the chrysanthemum can be effectively kept.

The present invention discloses an SNP molecular marker related to the pedicel hardness trait of Oncidium and its application. The nucleotide sequence of the molecular marker is shown as SEQ ID NO.1, wherein the SNP locus is at the 501bp of the sequence shown as SEQ ID NO.1, with A / G polymorphism. Through the simplified genome sequencing data of 106 Oncidium germplasm resources and combined with the phenotypic data related to the pedicel hardness of Oncidium, the present invention conducts a genome-wide association analysis, locates and screens out an SNP molecular marker related to the pedicel hardness. This molecular marker plays a key role in the regulation of the pedicel hardness trait of Oncidium, can be used for map-based cloning and molecular marker-assisted selection, so as to effectively screen out Oncidium varieties with stronger pedicel hardness at the seedling stage or non-flowering stage, accelerate the breeding process of Oncidium cut flower varieties, and is of great significance to the protection of germplasm resources and the breeding of new varieties.

The invention relates to the technical field of plant effective component extraction, in particular to an extraction method of a freeze-dried fresh rose stock solution, which comprises the following steps: selecting fresh fresh rose without plant diseases and insect pests, removing calyx, pedicel and other impurities, carrying out ultrasonic cleaning for 2-3 times by using a deionized water mixed solution containing citric acid, vitamin C and EDTA-2Na, and carrying out freeze-drying to obtain the freeze-dried fresh rose stock solution. After draining, the water content is controlled to be 86-88% through near infrared spectrum monitoring; wherein the content of citric acid in the deionized water mixed solution is 0.1%-0.3%, the content of vitamin C is 0.05%-0.08%, the content of EDTA-2Na is 0.02%-0.04%, the citric acid inhibits the activity of polyphenol oxidase, the vitamin C blocks an oxidation chain reaction, the EDTA-2Na chelates metal ions and avoids catalytic oxidation of the metal ions, and the oxidation rate of the active ingredients is reduced to 5% or below through synergism of the citric acid, the vitamin C and the EDTA-2Na.

The invention provides pogostemon cablin extracellular vesicles and an extraction and separation method and application thereof. Pogostemon cablin tissue juice is subjected to differential centrifugation, PEG precipitation and ultrafiltration, and the pogostemon cablin extracellular vesicles are extracted. The method is simple and convenient, equipment and materials are easy to obtain, the obtained extracellular vesicle crude extract is purified by combining an ultrafiltration method, and the obtained purified solution is clear and transparent and high in concentration and purity. The compound has no toxicity to HaCaT human immortalized epidermal cells and zebrafish, has good biocompatibility, shows good anti-photoaging, anti-oxidation and anti-inflammatory effects in in-vitro and in-vivo experiments, and is expected to be prepared into an anti-photoaging natural plant drug.

The invention discloses a fruit calyx / stem and surface defect high-precision identification method for enhancing polarization characteristic difference. The method comprises the following steps of: calculating polarization degree images and horizontal polarization enhancement images of calyx, fruit stem and defect areas according to acquired polarization images at four angles; preprocessing the polarization degree image and the horizontal polarization enhanced image by using median filtering and histogram equalization, then constructing a polarization characterization parameter fusion algorithm, and outputting a polarization parameter fusion image; and establishing a multi-modal model for detecting the calyx, fruit stem and defect area based on the polarization parameter fusion image and the visible light image. According to the method, the limitation of high false recognition rate of the traditional visible light image detection of the calyx fruit stems and the defects is broken through, and the multi-mode calyx fruit stem and defect detection method is constructed by using the polarization representation fusion image and the visible light image. Meanwhile, the method is simple and convenient in device and low in cost, and can be applied to an actual fruit production grading production line.

The invention relates to the technical field of breeding, in particular to application of rice PAL2 protein in regulation and control of panicle types or yield of plants. The spike type comprises one or more of the lowermost internode length of spike stalks, the internode length of branches, the pedicel length of spikelet at the top end, the spike length, the number of first-stage branches, the number of second-stage branches or the number of spike grains. The rice PAL2 protein related to the panicle type of the plant is researched and screened, and the panicle type character and yield of the plant can be effectively regulated and controlled through gene editing of the gene in the plant. The rice PAL2 protein provided by the invention can be applied to germplasm resource improvement of plants, and has an important value in the field of plant breeding.

The application belongs to the technical field of molecular markers, and relates to a SNP marker related to the length of a male flower stalk of watermelon and application thereof. The SNP marker is a single nucleotide at the 15321651th position of the 10th chromosome of the genome V2 of watermelon 97103, and the nucleotide is C or T. The length of the male flower stalk of watermelon with the TT genotype of the SNP site is longer than 12.00 mm, and the length of the male flower stalk of watermelon with the CC genotype of the SNP site is less than or equal to 12.00 mm. By comparing the genotype and the actual length of the male flower stalk, the accuracy of the SNP marker provided by the application in detecting the length of the male flower stalk of watermelon is 95.6%. By judging the genotype of the SNP site, the length of the male flower stalk of watermelon can be accurately and quickly identified at the seedling stage without the male flower growing on the plant. The breeding efficiency can be improved, the breeding time can be shortened, and the application is suitable for genetic identification and breeding of watermelon.

The invention relates to a facility pollination robot shaking, blowing, spraying and dipping integrated multiplex execution tail end which comprises a shell, a blower device and an air duct, a central spindle extending in the axial direction is fixedly arranged in the air duct, a spiral blade is fixedly arranged on the outer surface of the central spindle, and an air cavity is formed among the central spindle, the spiral blade and the inner wall of the air duct; a micro motor is installed in an inner hole of the mandrel, an output shaft of the micro motor is detachably and fixedly connected with a transmission rod, the end, away from the micro motor, of the transmission rod is fixedly connected with a rotary mesh enclosure, the rotary mesh enclosure is located on the air outlet side of the air cavity, and the output shaft of the micro motor, the transmission rod and the rotary mesh enclosure are coaxially arranged. The three pollination modes of air blowing, vibration and flower dotting are respectively aimed at different conditions in actual production of facility agriculture, under the condition that the temperature and illumination intensity in the facility are good, the machine recognizes flower clusters and pedicels to carry out air blowing or vibration pollination on crops, and when the temperature and illumination conditions in the facility are poor, the machine recognizes a single flower and carries out spraying and dipping pollination on the single flower.

The utility model discloses a chrysanthemum indicum flower stripping machine, and mainly relates to a flower stripping machine. Comprising an adjusting device, the adjusting device comprises a connecting plate, the connecting plate is in sliding connection with a feeding mechanism, a screw penetrates through the inner wall of the connecting plate in a threaded mode, the screw is in threaded connection with the feeding mechanism, a cutting groove is formed in one side of the connecting plate, and a supporting plate is fixedly connected to one end of the connecting plate; the cross section of the supporting plate is in an L shape, a motor is fixedly connected to one side of the short arm end of the supporting plate, the output end of the motor is fixedly connected with a rotating wheel, and the tooth surface of the rotating wheel is meshed with an adjusting wheel. The chrysanthemum indicum flower stripping machine has the beneficial effects that when chrysanthemum indicum is put into the flower stripping machine through the feeding mechanism, the pedicel of the chrysanthemum indicum can be cut through the adjusting device, so that the length of the pedicel can be reduced through cutting, and the chrysanthemum indicum can enter the flower stripping machine more conveniently; and furthermore, the efficiency of the whole flower removing machine for removing flowers from the wild chrysanthemum flowers is improved.

The invention belongs to the technical field of landscape plant physiological regulation and control, and provides a Japanese evening primrose single-side bud removal control experiment method based on physical regulation and control, which comprises the following steps: selecting Japanese evening primrose plants which grow vigorously and are free from diseases in a bud period, and randomly dividing the Japanese evening primrose plants into an experiment group and a control group; performing physical single-side leaf bud manual removal treatment on the plants in the experimental group, retaining the other side as a self-control group, and keeping the plants in the control group in a natural growth state; the method comprises the following steps: respectively collecting flower organ samples of an experimental group treatment side, an experimental group natural side and a control group in an initial blooming period, a blooming period and a littering period of Japanese evening primrose; measuring the flowering time, the RHS flower color value, the petal surface area, the pedicel length and the fresh flower dry weight index of the three groups of samples; according to the method, the ornamental period of the evening primrose is remarkably prolonged through physical bud removal treatment, the petal color is deepened, the petal surface area is increased, the flower visual effect is improved, the landscape layering sense is enhanced, and a scientific method is provided for management and application of ornamental tree species.

A control unit (63) recognizes, through image recognition, a harvest object (7), a pedicel (6a) connected to the harvest object, and a main stem (6a) connected to the pedicel. The control unit (63) determines a cutting position of the pedicel when the pedicel is included in a cutting region (Ra) for cutting the pedicel in the recognized image, and an overlapping ratio of the main stem with respect to the cutting region is less than an overlapping threshold.

The invention discloses a method for processing gardenia by adopting a vacuum low-temperature freeze-drying technology, a dried gardenia product and application, and belongs to the technical field of food processing. The method comprises the following steps: picking fresh cape jasmine flowers which bloom initially, removing impurities such as pedicels, sepals and leaves, and cleaning for later use; withering, draining, dishing, and carrying out low-temperature quick-freezing; transferring the quick-frozen gardenia to a freeze dryer, and performing freeze drying; and packaging. The invention also discloses the dried gardenia product prepared by the method, and application of the dried gardenia product in preparation of tea drink raw materials, flower substitutional tea, aromatherapy products and handicrafts. The dried gardenia processed by the method can retain more than 90% of original forms and active components, is good in rehydration effect, sweet, mellow and full in taste and resistant to storage and transportation, can be directly used as a novel tea drink raw material, can be directly brewed with flower substitutional tea, can be applied to application scenes such as aromatherapy products and handicrafts, and is suitable for commercial production or family small-scale production.

The utility model discloses a dry pedicel separating device of traditional Chinese medicinal materials, including device main part, annular receiving groove, mesh cylinder, hot air pipe and heating box, mesh cylinder is provided in device main part, annular receiving groove is provided outside mesh cylinder, the top end of device main part is provided with device cover body, the device cover body is provided with the hot air pipe, the hot air pipe is provided with the hot air pipe, the heating box is provided with the hot air pipe. The hot air pipe is installed in the device cover body, the hot air pipe extends into the net cylinder and is provided with branch pipes at equal intervals, an air conveying hose is installed at the top end of the hot air pipe, a motor is installed at the bottom of the device body, and the output end of the motor extends to the position below the net cylinder and is provided with a rotating disc; the heating box is arranged on one side of the device body, heating rods are installed in the heating box, and an induced draft fan is installed at the top end of the heating box. The device not only accelerates the separation of flowers and stems, improves the separation efficiency, accelerates the drying speed by continuously blowing hot air to traditional Chinese medicinal materials, but also is convenient for taking out impurities.

The utility model relates to the technical field of fresh cut flower sorting equipment, in particular to an automatic warehouse dividing structure for fresh cut flowers. The automatic warehouse dividing structure for the fresh cut flowers comprises a warehouse dividing pushing assembly and at least one warehouse dividing runner module, the warehouse dividing pushing assembly is installed on a main line rack and located below a main line carrier, and the warehouse dividing pushing assembly pushes the fresh cut flowers of the main line carrier into a warehouse dividing runner; the bin dividing flow channel module comprises a bin dividing rack, a plurality of bin dividing flow channels are installed on the bin dividing rack, each bin dividing flow channel comprises an oblique line section and a horizontal section, the oblique line sections are obliquely arranged, the high ends of the oblique line sections are located at the discharging end of the fresh cut flower sorting main line, and the low ends of the oblique line sections are connected with the horizontal sections; rubber strips are arranged on the portions, making contact with pedicels, of the branch bin flow channels, and nylon cloth wraps the rubber strips. By optimizing the design of the cabin kicking mechanism and the cabin separating flow channel, the automatic cabin separating operation with low flower loss, high efficiency, high flexibility and man-machine friendliness is realized.

The invention relates to the technical field of picking equipment, and discloses a marigold gripper which comprises a mounting bottom plate, four stand columns are connected to the bottom of the mounting bottom plate, the bottom ends of the four stand columns are connected with a fixing plate, four connecting rods are hinged to the fixing plate, and gripping flaps are connected to the connecting rods; the rotating assembly is used for driving the four grabbing petals to perform picking operation; the shearing assembly is used for applying shearing force to pedicels of the marigold when the marigold is picked. Through the arrangement of a rotating assembly, when a rotating block rotates, four arc-shaped pieces drive four connecting rods and four grabbing petals to conduct opening and gathering actions, so that the grabbing action on marigold flowers is achieved, automatic picking is achieved, and the picking efficiency is improved; through the cooperation of a curved groove ring and an adjusting rod, the grabbing force of the four grabbing petals can be adjusted according to the picking requirement, and therefore various picking methods that only petals are picked and pedicels are picked together are met.

A muskmelon flower castration device comprises a shell, a pedicel holding device, a female flower stigma protection cylinder, a male flower cutting device and a controller, the interior of the shell is divided into a cutting cavity and a device fixing cavity through an annular partition plate, the cutting cavity is located in the center of the shell, and the device fixing cavity is located in the center of the shell. The female flower stigma protection barrel is fixed to the top end of the cutting cavity, the pedicel holding device and the male flower cutting device are both fixed to the annular partition plate, the pedicel holding device is arranged below the male flower cutting device, a control switch is arranged at the top end of the shell, the controller is arranged at the upper end of the interior of the shell, and the control switch is connected with the controller. And the control device is connected with the pedicel holding device, the male flower cutting device and the control switch. According to the castration device, male flowers can be accurately removed at a time, time and labor are saved, the flexible air bag is inflated to hold pedicels tightly, and the pedicels are effectively prevented from being damaged.

The invention discloses a method for screening chrysanthemum varieties suitable for efficient harvesting. The method comprises the steps that the receptacle diameter D and the pedicel diameter d are measured; calculating the conformity size of the flower, wherein the conformity size of the flower is equal to D-d; the proportion of the intact flowers obtained through pre-harvesting in all the harvested flowers is counted, wherein the intact rate of the flowers is equal to n / N; the tensile stress of each level of node of the chrysanthemum plant is measured in the upward direction perpendicular to the ground from the top end to the base part, a function formula is fitted to obtain a correlation constant, the tensile force borne by a main stem is predicted, and the firmness of the main stem is evaluated. According to the method, the adaptability of the chrysanthemum plants to pulling type harvesting is analyzed on the basis of the diameter of receptacles and pedicels of the chrysanthemum plants, the tensile stress of branch nodes at all levels and other data, and the method is efficient and convenient; according to the method, the overall mechanical strength of the chrysanthemum plant is analyzed based on the function model, and complexity is described through a single index, so that understanding and screening are facilitated; the method is suitable for different varieties of chrysanthemum plants and has universality.

This invention provides the application of a protein negatively regulating the cucumber pedicel length trait, or the gene encoding said protein, in the growth of cucumber pedicels, belonging to the field of gene editing technology. In this invention, the protein CsIAA16 and its encoding gene are closely related to the pedicel length of cucumbers and the pedicel length of female cucumber flowers. This invention uses the CRISPR / Cas9 system combined with Agrobacterium-mediated genetic transformation technology to perform targeted genome editing on wild-type cucumbers, successfully constructing two homozygous mutant lines. Compared with the wild type, the female flower pedicels of the two knockout mutants are significantly longer. This invention utilizes the protein CsIAA16, or the gene encoding said protein CsIAA16, which negatively regulates the cucumber pedicel length trait. CsaV3_1 G0 1520 CsaV3_1 G0 152 It can lengthen the cucumber stem, which helps in the development of cucumber varieties suitable for mechanized harvesting.

The application belongs to the technical field of blueberry cultivation, and discloses a blueberry callus induction method, which comprises the following steps: (1) selecting blueberry flower buds 1-3 days before field flowering, removing petals and stamens, and placing the ovary with the stigma and pedicel retained in a 75% ethanol solution for sterilization for 10-15 min; (2) under aseptic conditions, the ovary with the stigma and pedicel retained is cut to remove the stigma, 1 / 4 of the ovary and the receptacle, and the ovule surrounded on the placenta is carefully taken out with sterilized tweezers and inoculated into a callus induction medium; after 30 days of dark culture, callus is obtained; the application provides a blueberry callus induction method, and has the technical effect of improving the callus induction rate of blueberries.