31 results about "Venom" patented technology

The invention belongs to the technical field of biological medicine, discloses a novel venom polypeptide composite dendrimer as well as a preparation method and application thereof, and particularly discloses scorpion venom polypeptide, and the amino acid sequence of the scorpion venom polypeptide is FLGGSLSSIF. According to the invention, a novel scorpion venom polypeptide (marked as C9) is separated and identified, and a novel polypeptide-polyamidoamine dendritic compound G5C9 (i.e., nanoparticles) with a glioblastoma (GBM) targeting effect is constructed. Compared with the polypeptide C9, the cell uptake efficiency of the G5C9 is obviously improved. G5C9 can enter cells through endocytosis and target lysosome to destroy functions of the lysosome and mTORC1, so that nuclear translocation of TFEB is promoted, and autophagic death of the cells is induced. Therefore, the G5C9 provides a novel mode for targeting the anti-GBM in vitro and in vivo.

The invention discloses snake and insect bite venom diffusion prevention emergency equipment based on a field operation environment, and belongs to the technical field of outdoor rescue equipment.The snake and insect bite venom diffusion prevention emergency equipment comprises a binding belt assembly, a mounting base and a blood sampling assembly.The binding belt assembly comprises a binding belt and air bags arranged on the two sides of the lower surface of the binding belt; the mounting base is arranged in the middle of the bandage and located between the two sets of air bags, the blood sampling assembly is detachably mounted in the mounting base, movable pressure adjusting mechanisms are arranged on the two sides of the mounting base, and the blood sampling assembly is provided with an arc-shaped blood sampling pipeline; through the synergistic effect of the pressure adjusting mechanism and the driving mechanism, dynamic adjustment of the pressure of the air bag is achieved, and tissue damage caused by continuous high pressure is avoided. The pressure cannot be adjusted in real time in a traditional bundling mode, however, according to the device, a motor drives a protruding block to be linked with a push plate, periodic change of the pressure is achieved, venom diffusion is effectively controlled, and smooth local blood circulation is guaranteed.

The present disclosure is a peptide (Inpén SEQ ID N ° 1) with whitening action for melasma, a cosmetic or dermopharmaceutic composition containing this peptide as active ingredient and the use of this composition in the treatment of melasma or skin hyperpigmentation, developed from a peptide bioinspired from the venom of Brazilian wasps. Melasma is an acquired chronic dermatologic disorder characterized by the emergence of hyperpigmented skin spots. Although it is a common condition, a definitive cure has not been developed so far, due to the complexity of multifactorial causes. Because it is a cosmetic composition, of topic and painless application, the present disclosure can be applied daily, with no need for a specialist in the field of aesthetics, besides not being expensive as the known to date techniques for treating melasma are. The peptide SEQ ID N° 1 acts inhibiting melanogenesis.

The application discloses a snake and insect bite venom diffusion prevention emergency equipment based on a field environment and belongs to the technical field of outdoor rescue equipment, which comprises a bandage assembly, a mounting seat and a blood sampling assembly. The bandage assembly comprises a bandage and air bags arranged on the lower surface of the bandage. The mounting seat is arranged at the middle part of the bandage and between the two groups of air bags. The blood sampling assembly is detachably mounted in the mounting seat. The mounting seat is provided with movable pressure adjusting mechanisms on both sides. The blood sampling assembly has an arc-shaped blood sampling pipeline. Through the cooperation of the pressure adjusting mechanisms and the driving mechanisms, the dynamic adjustment of the air bag pressure is realized, and the tissue damage caused by the continuous high pressure is avoided. The traditional bundling method cannot adjust the pressure in real time. Through the linkage of the motor driving protrusions and the push plates, the periodic change of the pressure is realized, the venom diffusion is effectively controlled, and the local blood circulation is ensured to be smooth.

The claimed method involves fractionation of IgGs by precipitation of Albumin and other low molecular weight proteins by mixing 3.5% Cparylic Acid with the plasma of hyper immunized horses. The selective microfiltration with 0.22 μm and higher pore filtration with PES sheets makes the end product more refined by removing the unrequited higher molecular weight proteins even the mega molecule IgE and IgM antibodies. On the other hand the ultra filtration with 100 KDa and lesser pore size enables exclusion of unwanted low molecule proteins like fibrinogen and others. The natural Virucidal quality of Caprylic Acid automatically decontaminates the end product without involving any additional or special procedure thus making the product safer by eliminating any transmittable zonotic virus / es present in the blood of immunized horses in to the body of patient treated with ASV. It is therefore natural properties of Caprylic Acid for precipitating Albumin and other low molecular weight proteins while retaining behind the required Immunoglobulin G antibodies are used as core technology together with some other simple allied techniques of direct and tangential filtration, PH maintenance at various stages for preserving the affinity efficacy of the antibodies and adding preservatives for longer shelf life makes this Novel method / formulation an appropriate technology for low or middle income countries (LMIC).

The present application relates to a kind of HhUp140 of anthomyiidae trichogramma venom protein and its application, it relates to the field of protein engineering, its amino acid sequence is as shown in SEQ ID NO:1, the protein can be used to paralyze pest larvae, extend pest pupation period.The present application extends the pupation period of anthomyiidae trichogramma venom protein HhUp140, reduces the disease of crop by pest to some extent.

A venom-based peptide and an application thereof, which relate to the technical field of biomolecules. The amino acid sequence of the peptide is represented by formula 1: X1-X2-X3-X4-X5-X6-X7-X8-X9-X10-X11-X12-X13-X14-X15-X16-X17-X18; X1, X5, X6, X9 and X11 are each selected from any one among A, V, L, I, M, F, W and P; X2, X3, X10, X15, X17 and X18 are each selected from any one among G, C, S, T, Y, N and Q; X7 is selected from D or E; X4, X8 and X16 are each selected from any one among K, R and H; and X12, X13 and X14 are each selected from any one among K, R, H, D and E. The peptide has the function of promoting self-renewal of human embryonic stem cells.

The designed functional module (2) is intended to be moved to the batching station (3) located in the processing area (93) of the containment (9), and sterile liquid is injected into the container there. The containment (9) is provided in the installation area (8) for positioning purposes, wherein the containment (9) and the processing area (93) are surrounded by the housing (90). The functional module (2) includes an element carrier (23), functional elements (230) for mounting on the element carrier (23), and a medium pipeline (231) connected to the functional elements (230). The assembled assembly composed of the element carrier (23), the functional elements (230) accommodated by the element carrier, and the medium pipeline (231) connected to the functional elements can be moved from the retracted initial position to the extended working position on the batching station (3) through the transfer port (260) leading to the processing area (93). The functional module (2) is used for aseptically filling liquid medicine or venom into a plurality of containers, and these containers are combined in a reservoir. In the second embodiment, the functional module (2) has an airtight container (28) with a sterilized inner chamber (29), in which an assembled and sterilized assembly is arranged, and the assembly includes an element carrier (23), functional elements (230) accommodated by the element carrier, and a medium pipeline (231) connected to the functional elements. Finally, a method for moving the functional module (2) to the batching station (3) located in the processing area (93) is proposed.

The present invention relates to insulin analogs, particularly insulin analogs having shortened B chains. The present invention also relates to the crystal structure of insulin from the venom of cone snails and to methods of using the crystal and related structural information to screen for and design insulin analogs that interact with or modulate the insulin receptor. The present invention also relates to therapeutic and prophylactic methods using insulin analogs.

A bacterial enrichment solution and its use in detecting Burkholderia gladioli (Pseudomonas cocovenenans subsp. venom), the bacterial enrichment solution containing the following components: a potato dextrose aqueous solution, gentian violet, chloramphenicol, polymyxin B, and sodium dehydroacetate. The bacterial enrichment solution has a good bacterial enrichment effect on target strains and can effectively inhibit the growth of non-target bacteria that appear frequently during routine detection, reducing interference from non-target bacteria and improving the detection rate and work efficiency of target bacteria. The present invention has a simple preparation process, low cost, and strong operability, and is suitable for enriching Burkholderia gladioli (Pseudomonas cocovenenans subsp. venom) in various foods.

The invention relates to the technical field of functional protein screening, in particular to an effector with a venom protein function and application thereof. The invention provides an effector with a venom protein function. The effector comprises an A0A2W1B606 protein, an A0A2W1BGQ7 protein, an A0A2W1BE44 protein, an A0A2W1B9V0 protein, an A0A2W1BFA8 protein or an I3QC11 protein. The invention further provides a preparation method of the effector. The effector obtained by screening has high-level metabolic activity, signal transduction function and venom protein function; the method plays a key role in the interaction process of phytophagous insects-host plants, such as participation in the process of cotton bollworm invading plants, regulation of the resistance of the plants to cotton bollworm larvae, targeted inhibition of phenylpropane metabolic pathways, induction of cotton bollworm preference colonization and the like.

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a metarhizium anisopliae engineering bacterium for expressing spider venom peptide Hv1a and application of the metarhizium anisopliae engineering bacterium in prevention and control of chilo suppressalis. The invention discloses the obvious insecticidal activity of Metarhizium guerizense ARSEF 977 on chilo suppressalis for the first time, on the basis, an Hv1a toxic peptide gene derived from a spider is introduced into the strain through a genetic engineering technology, an engineering bacterium for stably and efficiently expressing the spider insecticidal peptide Hv1a is successfully constructed, the engineering bacterium plays a synergistic effect, the insecticidal effect on the chilo suppressalis is further improved, and the application prospect is wide. The death rate after 7 days of treatment reaches up to 96.7%, and the method can be used for controlling chilo suppressalis. The invention provides a high-potential starting strain for developing a special fungal insecticide for chilo suppressalis, provides a new material and method for promoting the development of a green and efficient chilo suppressalis prevention and control technology, and has a wide application prospect.

The invention belongs to the technical field of poultry vaccines, and particularly relates to a chicken new tributary method adeno-quintuplet vaccine and a preparation method thereof.The chicken new tributary method adeno-quintuplet vaccine comprises chicken Newcastle disease venom, infectious bronchitis venom, avian influenza venom, infectious bursal disease virus liquid and fowl adenovirus venom, and the preparation method of the Newcastle disease venom comprises the steps that firstly, frozen MDCK suspension cells are provided; the frozen MDCK suspension cells are recovered at the temperature of 40-45 DEG C; 2, transferring the resuscitated MDCK suspension cells to a serum-free culture medium; according to the invention, the inactivated vaccine is prepared from the Newcastle disease venom, the infectious bronchitis venom, the avian influenza venom, the infectious bursal disease virus liquid and the fowl adenovirus venom by adopting a superstrong concentration process and a high-quality adjuvant, so that after animals are immunized, antibodies are generated quickly, the titer is high, the protection period is long, and the occurrence and spreading of epidemic diseases are prevented; and people can conveniently put multi-combined vaccines to cope with continuously changing epidemic disease prevention and control situations.

The invention discloses an arginine derivative modified on the basis of wasropetin and application of the arginine derivative, and belongs to the technical field of antibacterial peptides. The arginine derivative is one of the following two: Arg < 2 > MP-C and Arg < 4 >. 11.12 MP-C; the amino acid sequence of the Arg2MP-C is as shown in SEQ ID NO. 2; the amino acid sequence of the Arg4. 11.12 MP-C is as shown in SEQ ID NO. 3. The invention further discloses application of the arginine derivative modified on the basis of the wasropetin in serving as or preparing antibacterial drugs. The invention further discloses application of the arginine derivative modified on the basis of the wasropetin. According to the invention, arginine is introduced into MP-C, so that the antibacterial activity is improved. The arginine derivative disclosed by the invention is good in bactericidal effect, low in risk of drug resistance of bacteria and huge in application potential, and has important significance on research and commercialized production of antibacterial peptides.

The application relates to the field of biological medicine, and provides an application of kaempferol or a Huaqinjiequ Decoction containing the same in the preparation of a jellyfish stinging treatment drug. The protection effect and mechanism of the Huaqinjiequ Decoction (HLJDT) on heart injury induced by Stomolophus meleagris venom (TE) are explored through in-vivo and in-vitro experiments. The results show that the HLJDT can significantly improve the cell survival rate and the survival rate of mice under TE exposure, reduce the abnormality of myocardial enzyme spectrum indexes, relieve the over-activation of the MAPK pathway and the expression of inflammatory factors, and improve the pathological injury of heart tissue. In combination with in-vivo and in-vitro activity evaluation, molecular docking and molecular mechanism research, it is further shown that kaempferol can effectively antagonize the myocardial cell toxicity caused by TE, and the mechanism is related to the inhibition of the MAPK signal axis. In summary, kaempferol is an important material basis for the Huaqinjiequ Decoction to antagonize inflammatory response and oxidative damage. It also plays an important role in reducing the myocardial cell toxicity and overall toxic effect induced by TE, and provides an experimental basis for preventing and treating jellyfish stings by using the traditional Chinese medicine compound modified HLJDT.

The invention discloses an application of six subunit proteins of sebastibastes roseus venom protein neoVTX. The invention finds that six subunit proteins of neoVTX can inhibit voltage-gated sodium ion channels and mediate muscle paralysis functions, and verifies that neoVTX can inhibit activities of zebra fish and grass goldfish and does not cause individual death. The invention provides a new direction for preparing a voltage-gated sodium ion channel macromolecular probe template and a muscle paralysis drug by using six subunit proteins of the sebastites verrucosa venom protein neoVTX.

The invention discloses a large-scale production and inactivation process for foot and mouth disease viruses. After standardization of an inactivator cyclization method is established, storage time and temperature of the inactivator are optimized, toxicity test of 50% Na2S2O3 on BHK-21 cells and dosage optimization of inactivated 50% Na2S2O3 in mass production are carried out, and different foot-and-mouth disease virus strains TCID50 and inactivation kinetics curves are established, the method is applied to large-scale production. After cyclization of the inactivator, whether BEA cyclization succeeds or not is judged by detecting pH and color change of a beta-naphthol purple indicator, thorough inactivation of venom is further ensured, biological safety is improved, meanwhile, it is determined that the inactivator can be stored at the temperature of 2-8 DEG C, and the storage life is smaller than or equal to 14 d; 50% of Na2S2O3 is not added after venom inactivation is finished, so that side reactions after vaccination are reduced, the safety of vaccination is improved, and the production cost of the vaccine is reduced; the inactivation process can be accurately controlled, the inactivation efficiency can be improved, the complete inactivation of viruses can be ensured, the biological safety risk in vaccine production can be avoided, the inactivation time can be shortened, and the production cycle can be shortened by drawing the inactivation power curve of different strains.

The invention relates to molecular biology and genetic engineering technology, and aims to provide a kind of antijatasia venom protein gene VID and application thereof in degradation of drosophila melanogaster tissues. The venom protein genes of the antijatropatella gigantean are named as a venom protein gene VID-1, a venom protein gene VID-2, a venom protein gene VID-3, a venom protein gene VID-4 and a venom protein gene VID-5 respectively, and the nucleotide sequences of the venom protein genes are shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5 respectively. The invention discloses the regulation and control function of the antijatropha spp venom protein on the in-vivo tissues of the drosophila larvae for the first time, and experiments verify that the venom protein can specifically induce host adult disc tissues to degrade. Researches prove that the antijatropha opening venom protein can degrade adult disc tissues to prevent fruit flies from eclosion so as to realize pest killing, so that the antijatropha opening venom protein has the value of being applied to agricultural biological control.

The utility model relates to the field of insect collection, and discloses a poison bottle for collecting insects, which comprises a bottle body, a protective sleeve is detachably mounted on the outer wall of the bottle body, a placing block is fixedly connected to the outer wall of the protective sleeve, tweezers are detachably mounted on the inner wall of the placing block, a strap is arranged on the outer wall of the bottle body, and the strap is fixedly connected to the outer wall of the protective sleeve. Rotary covers are detachably mounted on the outer walls of the upper end and the lower end of the bottle body through external threads, degreasing cotton is detachably mounted on the inner wall of the rotary cover at the lower end, a connecting disc is fixedly connected to the bottom end of the outer wall of a connecting rod, and a valve is arranged on the inner wall of the top end of a rotating block. According to the insect fumigating device, the through hole in the connecting disc is aligned with the liquid outlet in the bottom end of the toxic agent storage chamber by rotating the rotating block, toxic liquid flows into the degreasing cotton and then enters the insect fumigating chamber through the small air holes in the surface of the insect fumigating chamber to kill insects, the situation that liquid agents directly flow into the insect fumigating chamber to cause adhesion damage of insect bodies is avoided, and the integrity of the insect bodies is guaranteed; and subsequent observation and research are facilitated.

The invention belongs to the field of biological medicine technology and food, and discloses application of scorpion toxin in preparation of weight-reducing medicine, and the scorpion toxin can be applied to preparation of the weight-reducing medicine, preparation of products for controlling fat accumulation in organisms, preparation of functional food for weight management and preparation of products for reducing the weight of the organisms. According to the application disclosed by the invention, the scorpion toxin can be used for reducing the body weight of an organism, reducing triglyceride and total cholesterol levels in serum, effectively relieving obesity and improving metabolic conditions such as glucose tolerance and insulin resistance, a new thought is provided for preventing and treating obesity and metabolic complications thereof, and the scorpion toxin has a wide clinical application prospect.

Provided are a new venom polypeptide-dendrimer complex, and a preparation method therefor and the use thereof. Specifically, provided is a scorpion venom polypeptide, wherein the amino acid sequence of the scorpion venom polypeptide is FLGGLLSSIF. A new scorpion venom polypeptide (designated as C9) is isolated and identified. Moreover, a new polypeptide-polyamidoamine dendrimer complex G5C9 (i.e., nanoparticles) having a glioblastoma (GBM)-targeting effect is constructed. Compared with polypeptide C9, G5C9 exhibits significantly improved cellular uptake efficiency. G5C9 can enter cells via endocytosis and targets lysosomes, and inhibits lysosomal function and mTORC1, thereby promoting nuclear translocation of TFEB and further inducing autophagic cell death. Therefore, G5C9 provides a new targeted anti-GBM approach both in vitro and in vivo.

This invention belongs to the field of microbial technology, specifically disclosing a strain of *Beauveria bassiana* Bp-01, its recombinant strain, and its applications. The *Beauveria bassiana* Bp-01 provided by this invention, with the preservation number CGMCC No. 42442, exhibits strong pathogenicity against the red turpentine beetle and can be used as an excellent biocontrol strain. To further enhance its insecticidal virulence and infection rate, this invention utilizes genetic engineering techniques to successfully construct a recombinant strain Bp, with the preservation number CGMCC No. 42333, by overexpressing the venom protein SERCA in *Beauveria bassiana* Bp-01. Verification showed that compared to the wild-type strain, this recombinant strain infects *Red turpentine beetle* larvae faster, has a higher lethality, and significantly enhanced virulence, further demonstrating stronger infection and colonization capabilities. This invention can be applied to the preparation of biocontrol agents to achieve efficient control of the forest pest, the red turpentine beetle.

The invention relates to the field of vaccine manufacturing and preservation, in particular to a heat-resistant protective agent, a heat-resistant live vaccine as well as a preparation method and application thereof. The invention provides a heat-resistant protective agent for a heat-resistant canine distemper and canine parvovirus bivalent live vaccine. The heat-resistant protective agent comprises the following components in percentage by mass: 3%-30% of trehalose, 5%-25% of cane sugar, 1.5%-10% of glucan, 0.5%-2% of glycine, 0.5%-2% of histidine, 1%-5% of casein hydrolysate, 0.5%-5% of gelatin and the balance of water for injection. The invention also provides a preparation method of the heat-resistant canine distemper and canine parvovirus disease bivalent live vaccine, which comprises the following steps: uniformly mixing the protective agent with a canine distemper virus solution and a canine parvovirus solution to obtain a canine distemper and canine parvovirus disease bivalent live vaccine stock solution; and carrying out gradient vacuum drying to obtain the canine distemper and canine parvovirus disease bivalent live vaccine. The canine bivalent vaccine sugar glass preparation prepared by the invention has good heat resistance, and the heat resistance effect of the canine bivalent vaccine sugar glass preparation is superior to that of a traditional freeze-dried live vaccine product.

Provided herein are methods of treating or preventing venom induced injury, including local tissue damage and / or skin irritation using composition comprising an effective amount of heparin and / or heparinoid. Also provided are uses of said composition in the manufacture of a medicament for treating or preventing the same and said composition for use in treating or preventing the same.

The invention relates to the production and development of a lyophilized injectable formulation of modified antibodies, or their variants, that are highly specific neutralizers of heterologous mixtures of proteins, peptides, and other organic or inorganic components having different specific activities and which may include, but are not limited to, venoms of venomous animals. For convenience, we refer to venoms, but all types of venoms from terrestrial and marine animals are included. It also relates to a production process that includes the hyperimmunization of mammals for the production of highly specific antibodies, the modification (fragmentation) and purification process, and finally the injectable formulation, thus conferring high purity and high specificity properties.

The invention discloses an insect toxin protein and application thereof. The amino acid sequence of the insect toxin protein disclosed by the invention is any one of amino acid sequences as shown in SEQ ID NO.1-3. A new venom protein extraction method is adopted, new toxin which is not reported yet is identified, an insect toxin protein library is enriched, new protein is provided for the insect toxin, and the method can be applied to insect toxin research, insect evolution mechanism research and research on prevention and treatment of human or animal poisoning caused by insect toxin infection and has a wide application prospect. The method is also expected to be applied to preparation of protein biological products, such as antibacterial peptides, antiviral peptides and medical products, and has good application prospects in scientific research, production and life.

The invention provides a spicy stone fish premade dish and a preparation method thereof, and belongs to the technical field of fish premade dishes. The preparation method comprises the following steps: selecting healthy and qualified stone fish, removing gills of the stone fish, performing bloodletting, removing fish scales, removing viscera and simultaneously removing adeno-poison sacs of the stone fish to obtain the stone fish for later use; performing fishy smell removal treatment on the stone fish for later use to obtain fishy smell removed stone fish: frying the fishy smell removed stone fish to obtain the spicy stone fish after frying; and sterilizing the spicy stone fish and then storing the spicy stone fish to obtain the spicy stone fish prefabricated dish. The components of the fishy smell removing liquid are limited, the stone fish is subjected to efficient fishy smell removing treatment, and the final taste of the stone fish is guaranteed; meanwhile, the final spicy stone fish is sterilized by adopting an ultraviolet manner, so that all bacteria are killed before preservation, secondary fermentation is avoided in the preservation process, and the original flavor of the spicy stone fish is further preserved.

The invention relates to the technical field of medicines, in particular to application of a Pellino1 protein inhibitor or a derivative thereof in preparation of a medicine for treating and / or preventing jellyfish injury and the medicine for treating and / or preventing jellyfish injury. The benzonaphthalene lignan compound can be used for weakening downstream MAPK mediated jellyfish venom cell and animal toxicity by acting on Pellino-1 and reducing inflammatory response, and has potential medicinal value in treatment of jellyfish stings and toxic animal injury poisoning.