13 results about "Venom" patented technology

The invention discloses snake and insect bite venom diffusion prevention emergency equipment based on a field operation environment, and belongs to the technical field of outdoor rescue equipment.The snake and insect bite venom diffusion prevention emergency equipment comprises a binding belt assembly, a mounting base and a blood sampling assembly.The binding belt assembly comprises a binding belt and air bags arranged on the two sides of the lower surface of the binding belt; the mounting base is arranged in the middle of the bandage and located between the two sets of air bags, the blood sampling assembly is detachably mounted in the mounting base, movable pressure adjusting mechanisms are arranged on the two sides of the mounting base, and the blood sampling assembly is provided with an arc-shaped blood sampling pipeline; through the synergistic effect of the pressure adjusting mechanism and the driving mechanism, dynamic adjustment of the pressure of the air bag is achieved, and tissue damage caused by continuous high pressure is avoided. The pressure cannot be adjusted in real time in a traditional bundling mode, however, according to the device, a motor drives a protruding block to be linked with a push plate, periodic change of the pressure is achieved, venom diffusion is effectively controlled, and smooth local blood circulation is guaranteed.

The application discloses a snake and insect bite venom diffusion prevention emergency equipment based on a field environment and belongs to the technical field of outdoor rescue equipment, which comprises a bandage assembly, a mounting seat and a blood sampling assembly. The bandage assembly comprises a bandage and air bags arranged on the lower surface of the bandage. The mounting seat is arranged at the middle part of the bandage and between the two groups of air bags. The blood sampling assembly is detachably mounted in the mounting seat. The mounting seat is provided with movable pressure adjusting mechanisms on both sides. The blood sampling assembly has an arc-shaped blood sampling pipeline. Through the cooperation of the pressure adjusting mechanisms and the driving mechanisms, the dynamic adjustment of the air bag pressure is realized, and the tissue damage caused by the continuous high pressure is avoided. The traditional bundling method cannot adjust the pressure in real time. Through the linkage of the motor driving protrusions and the push plates, the periodic change of the pressure is realized, the venom diffusion is effectively controlled, and the local blood circulation is ensured to be smooth.

The claimed method involves fractionation of IgGs by precipitation of Albumin and other low molecular weight proteins by mixing 3.5% Cparylic Acid with the plasma of hyper immunized horses. The selective microfiltration with 0.22 μm and higher pore filtration with PES sheets makes the end product more refined by removing the unrequited higher molecular weight proteins even the mega molecule IgE and IgM antibodies. On the other hand the ultra filtration with 100 KDa and lesser pore size enables exclusion of unwanted low molecule proteins like fibrinogen and others. The natural Virucidal quality of Caprylic Acid automatically decontaminates the end product without involving any additional or special procedure thus making the product safer by eliminating any transmittable zonotic virus / es present in the blood of immunized horses in to the body of patient treated with ASV. It is therefore natural properties of Caprylic Acid for precipitating Albumin and other low molecular weight proteins while retaining behind the required Immunoglobulin G antibodies are used as core technology together with some other simple allied techniques of direct and tangential filtration, PH maintenance at various stages for preserving the affinity efficacy of the antibodies and adding preservatives for longer shelf life makes this Novel method / formulation an appropriate technology for low or middle income countries (LMIC).

The invention belongs to the technical field of biological prevention and control of agricultural pests, and particularly relates to a metarhizium anisopliae engineering bacterium for expressing spider venom peptide Hv1a and application of the metarhizium anisopliae engineering bacterium in prevention and control of chilo suppressalis. The invention discloses the obvious insecticidal activity of Metarhizium guerizense ARSEF 977 on chilo suppressalis for the first time, on the basis, an Hv1a toxic peptide gene derived from a spider is introduced into the strain through a genetic engineering technology, an engineering bacterium for stably and efficiently expressing the spider insecticidal peptide Hv1a is successfully constructed, the engineering bacterium plays a synergistic effect, the insecticidal effect on the chilo suppressalis is further improved, and the application prospect is wide. The death rate after 7 days of treatment reaches up to 96.7%, and the method can be used for controlling chilo suppressalis. The invention provides a high-potential starting strain for developing a special fungal insecticide for chilo suppressalis, provides a new material and method for promoting the development of a green and efficient chilo suppressalis prevention and control technology, and has a wide application prospect.

The invention belongs to the technical field of poultry vaccines, and particularly relates to a chicken new tributary method adeno-quintuplet vaccine and a preparation method thereof.The chicken new tributary method adeno-quintuplet vaccine comprises chicken Newcastle disease venom, infectious bronchitis venom, avian influenza venom, infectious bursal disease virus liquid and fowl adenovirus venom, and the preparation method of the Newcastle disease venom comprises the steps that firstly, frozen MDCK suspension cells are provided; the frozen MDCK suspension cells are recovered at the temperature of 40-45 DEG C; 2, transferring the resuscitated MDCK suspension cells to a serum-free culture medium; according to the invention, the inactivated vaccine is prepared from the Newcastle disease venom, the infectious bronchitis venom, the avian influenza venom, the infectious bursal disease virus liquid and the fowl adenovirus venom by adopting a superstrong concentration process and a high-quality adjuvant, so that after animals are immunized, antibodies are generated quickly, the titer is high, the protection period is long, and the occurrence and spreading of epidemic diseases are prevented; and people can conveniently put multi-combined vaccines to cope with continuously changing epidemic disease prevention and control situations.

The application relates to the field of biological medicine, and provides an application of kaempferol or a Huaqinjiequ Decoction containing the same in the preparation of a jellyfish stinging treatment drug. The protection effect and mechanism of the Huaqinjiequ Decoction (HLJDT) on heart injury induced by Stomolophus meleagris venom (TE) are explored through in-vivo and in-vitro experiments. The results show that the HLJDT can significantly improve the cell survival rate and the survival rate of mice under TE exposure, reduce the abnormality of myocardial enzyme spectrum indexes, relieve the over-activation of the MAPK pathway and the expression of inflammatory factors, and improve the pathological injury of heart tissue. In combination with in-vivo and in-vitro activity evaluation, molecular docking and molecular mechanism research, it is further shown that kaempferol can effectively antagonize the myocardial cell toxicity caused by TE, and the mechanism is related to the inhibition of the MAPK signal axis. In summary, kaempferol is an important material basis for the Huaqinjiequ Decoction to antagonize inflammatory response and oxidative damage. It also plays an important role in reducing the myocardial cell toxicity and overall toxic effect induced by TE, and provides an experimental basis for preventing and treating jellyfish stings by using the traditional Chinese medicine compound modified HLJDT.

The invention discloses an application of six subunit proteins of sebastibastes roseus venom protein neoVTX. The invention finds that six subunit proteins of neoVTX can inhibit voltage-gated sodium ion channels and mediate muscle paralysis functions, and verifies that neoVTX can inhibit activities of zebra fish and grass goldfish and does not cause individual death. The invention provides a new direction for preparing a voltage-gated sodium ion channel macromolecular probe template and a muscle paralysis drug by using six subunit proteins of the sebastites verrucosa venom protein neoVTX.

The invention relates to molecular biology and genetic engineering technology, and aims to provide a kind of antijatasia venom protein gene VID and application thereof in degradation of drosophila melanogaster tissues. The venom protein genes of the antijatropatella gigantean are named as a venom protein gene VID-1, a venom protein gene VID-2, a venom protein gene VID-3, a venom protein gene VID-4 and a venom protein gene VID-5 respectively, and the nucleotide sequences of the venom protein genes are shown as SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4 and SEQ ID NO: 5 respectively. The invention discloses the regulation and control function of the antijatropha spp venom protein on the in-vivo tissues of the drosophila larvae for the first time, and experiments verify that the venom protein can specifically induce host adult disc tissues to degrade. Researches prove that the antijatropha opening venom protein can degrade adult disc tissues to prevent fruit flies from eclosion so as to realize pest killing, so that the antijatropha opening venom protein has the value of being applied to agricultural biological control.

Provided are a new venom polypeptide-dendrimer complex, and a preparation method therefor and the use thereof. Specifically, provided is a scorpion venom polypeptide, wherein the amino acid sequence of the scorpion venom polypeptide is FLGGLLSSIF. A new scorpion venom polypeptide (designated as C9) is isolated and identified. Moreover, a new polypeptide-polyamidoamine dendrimer complex G5C9 (i.e., nanoparticles) having a glioblastoma (GBM)-targeting effect is constructed. Compared with polypeptide C9, G5C9 exhibits significantly improved cellular uptake efficiency. G5C9 can enter cells via endocytosis and targets lysosomes, and inhibits lysosomal function and mTORC1, thereby promoting nuclear translocation of TFEB and further inducing autophagic cell death. Therefore, G5C9 provides a new targeted anti-GBM approach both in vitro and in vivo.

This invention belongs to the field of microbial technology, specifically disclosing a strain of *Beauveria bassiana* Bp-01, its recombinant strain, and its applications. The *Beauveria bassiana* Bp-01 provided by this invention, with the preservation number CGMCC No. 42442, exhibits strong pathogenicity against the red turpentine beetle and can be used as an excellent biocontrol strain. To further enhance its insecticidal virulence and infection rate, this invention utilizes genetic engineering techniques to successfully construct a recombinant strain Bp, with the preservation number CGMCC No. 42333, by overexpressing the venom protein SERCA in *Beauveria bassiana* Bp-01. Verification showed that compared to the wild-type strain, this recombinant strain infects *Red turpentine beetle* larvae faster, has a higher lethality, and significantly enhanced virulence, further demonstrating stronger infection and colonization capabilities. This invention can be applied to the preparation of biocontrol agents to achieve efficient control of the forest pest, the red turpentine beetle.

The invention discloses an insect toxin protein and application thereof. The amino acid sequence of the insect toxin protein disclosed by the invention is any one of amino acid sequences as shown in SEQ ID NO.1-3. A new venom protein extraction method is adopted, new toxin which is not reported yet is identified, an insect toxin protein library is enriched, new protein is provided for the insect toxin, and the method can be applied to insect toxin research, insect evolution mechanism research and research on prevention and treatment of human or animal poisoning caused by insect toxin infection and has a wide application prospect. The method is also expected to be applied to preparation of protein biological products, such as antibacterial peptides, antiviral peptides and medical products, and has good application prospects in scientific research, production and life.