810 results about "Biologic Products" patented technology

Significant research is being done to develop and improve delivery mechanisms for biopharmaceuticals and vaccines, including pulmonary (inhalation), nasal, transdermal, and oral alternatives. Market projections indicate that the delivery of proteins and vaccines by inhalation and oral formulation has become and will continue to be increasingly important. These delivery mechanisms, to be effective, will require better stabilization of the biologicals so that they can maintain potency and effectiveness at ambient temperatures for extended periods of time. The novel Preservation by Vaporization (PBV) Technology described herein provides cost-effective and efficient industrial scale stabilization of proteins, viruses, bacteria, and other sensitive biologicals, thereby allowing a production of products that are not possible to be produced by existing methods. The suggested new PBV process comprises primary drying under vacuum from a partially frozen state (i.e. slush) at near subzero temperatures followed by stability drying at elevated temperatures (i.e., above 40 degrees Celsius). The new suggested method can be performed aseptically in unit doze format (in vials) and/or in bulk format (in trays, bags, or other containers). The drying can be performed as a continuous load process in a manifold vacuum dryer comprising a plurality (e.g., 30) of vacuum chambers attached to a condenser during the drying.

This invention relates to formulations and methods for preserving sensitive biologicals, viruses, bacteria and eukaryotic cells by drying. More particularly, the invention relates to preservation mixtures containing viruses or cells and protectants, including a combination of a methylated monosaccharide and a disaccharide, or oligosaccharide, wherein the mixtures are adapted to stabilize these during dehydration and subsequent storage at ambient and higher temperature.

Bioreactors suitable for housing a predetermined volume of liquid comprising nutrient medium and biological culture comprising: (a) a container having at least one interior wall; (b) at least one nutrient medium inlet; (c) at least one liquid outlet; (d) at least one gas inlet; (e) at least one gas outlet; and (f) at least one cylindrical sparging filter attached to the at least one gas inlet, wherein the sparging filter comprises a plurality of pores along its axis which permit gas to be emitted radially from the sparging filter into the liquid, wherein the diameter of the plurality of pores does not exceed about 50 μm, and wherein the orientation of the at least one sparging filter within the container provides for immersion of the plurality of pores within the liquid and substantially uniform distribution of emitted gas throughout the liquid, and related methods of using said bioreactors to prepare various biological products.

A flexible, multi-product, multi-technology, expandable facility for manufacturing products, such as biologicals, pharmaceuticals, or chemicals, and manufacturing processes using elements of such a facility.

The present invention is directed to the development of continuous processing technology for the purification of biopharmaceuticals and biological products, such as monoclonal antibodies, protein therapeutics, and vaccines. Methods for continuous processing of a biological product in a feed stream toward formulation of a purified bulk product are described.

Disclosed is a process of determining, at a point during shipment, whether the solid phase refrigerant in a shipment is sufficient to preserve the shipment cargo, which is blood or other biological products, for the remaining shipment period, by: monitoring the temperatures encountered to said point and estimating the temperatures likely to be encountered during the remaining shipment period; determining the likelihood that the remaining refrigerant can maintain the shipment cargo within a specified temperature range during the remaining shipment period; and if the risk that the remaining refrigerant cannot maintain the shipment cargo within said range during the remaining shipment period is above a cut-off level, then taking action to preserve the value of the cargo.

The invention relates to the culture medium research and development technical field of modern biological technology and provides a serum-free medium for MDCK cell large-scale adherent culture and single-cell suspension culture, which comprises 21 amino acids, 6 vitamins, 8 salts, 8 lipids, 4 trace elements, 2 buffers, 1 protein hydrolysate, 1 acid-base indicator and 6 other additives. The serum-free medium can be prepared by the conventional preparation method, and an application method thereof is the conventional method. The serum-free medium has the beneficial effects that: the serum-free medium does not contain serum, has clear components, is beneficial for separating and purifying the product and improves the product quality; the serum-free medium supports long-term subculture of MDCK cells and does not require long-term and complex adaptation process; and the serum-free medium can well support the adherent growth and single-cell suspension growth of the MDCK cells, has clear components and easy preparation and utilization, and is suitable for mass production of biological products.

A freeze-dried high-temp. resistant protecting agent for virus-type biologic products for veterinary medicine is prepared from several components through proportionally mixing. It features that each of its components is sterilized separately. For the components which can be sterilized under high temp., they are dissolved in distilled water according to the proportion of formulation and are sterilized at 116 deg.C for 30-40 min; and for those which do not resist the high temp., they are also dissolved in distilled water according the proportion of formulation and are filtered with 0.22 micron pore diameter filter membrane to remove bacteria; and then the two parts are mixed to obtain the freeze-dried high-temp. resistant, protecting agent. The mentioned two parts are added to the virus antigen liquid according to a proportion of 1:1 and after packaging, they are freeze-dried. The product can be stored at 2-8 deg.C for 24 months.

The invention discloses an organic recycling agriculture production system with high efficiency, which uses food obtained from planted crops for feeding livestock, manure produced by which is fermented so as to produce biogas, biogas residue and biogas slurry, wherein, the biogas can be used as clean energy, while the biogas residue and biogas slurry are applied to cultivated lands or grass lands; the planted forage and crop stalks can be used for raising cattle or earthworm after grinded together with cattle manure; the earthworm can be extracted to produce plant growth enhancer, protein feed and biologic products; the earthworm manure can be used as highly effective organic fertilizer to be applied to farm lands or grasslands. The organic recycling agriculture production system of the invention abides by the theory of agriculture circular economy, utilizes nature resource fully and reasonably, promotes resource regeneration, forms virtuous circle of multi-grade utilization of material and energy, and thus is a modern agriculture technique with high quality, high yielding, high efficiency, low consumption and environment protection as well.

The invention discloses a method for producing edible fungi flavoring powder, and belongs to the field of novel biological products. For solving the technical problems, the invention provides the method for producing the edible fungi flavoring powder for keeping the nutritional and functional components of fungi and keeping original flavor and form. The method comprises the following steps of: a, raw material treatment: cleaning and slicing at least one of fresh tricholoma matsutake, toadstool, bolete, sarcodon quel, hericium erinaceus, lentinus edodes, pleurotus cornucopiae, pleurotus eryngii, cantharellus cibarius, termitomyces albuminosus, agrocybe cylindracea, lactarius deliciosus and russulaceae and fresh truffle, and drying the surface moisture in air; b, freezing; c, vacuum drying; d, resolving and drying; e, crushing; and f, sterile packing. The flavoring powder produced by using the method can keep original flavor and form, the nutritional and functional components are not lost, the indexes of the stored flavoring powder are in accordance with the regulation of edible fungi health standard GB7096-2003, and the water content index of the flavoring powder is lower than 1/3 of the national standard.

The invention discloses a farm fertilizer produced by biologic technology, in particular a decomposing agent for rapidly decomposing straws at low cost, the mixture ratio of which is aerobic bacillus subtilis Cohn 20%, Bacillus licheniformis 10%, aspergillus flavus 20%, chaetomiaceae 20%, absidia corymbifera 20%, and saccharomycetes 10%. The production procedures are: extracting different beneficial decomposed bacteria from organics from different environments, purifying, rejuvenating, breeding and culturing; inoculating the cultured bacterial strains into disinfected solid culture medium; fermenting for 4-5 days under 25-50 DEG C, airing dry so that the residual moisture in the bacterial strains is 25%; mixing, agitating evenly and packing the aired solid bacterial strains into solid decomposing agent. As the invention is made by using a unique optimization and combination of aerobic bacterial group and a high and new technology and production process, the invention is a pure biologic product, is free from smell and harm for making organic fertilizer by effectively decomposing wastes in the countryside, and the fermenting speed is high, the cost is low, and the source of raw material is wide. The invention has improved the soil structure and reduces environmental pollution.

The invention relates to an animal Bifidobacterium and a use method thereof. The animal Bifidobacterium is the Bifidobactrium amimalis Subsp Lactis B174, the CGMCC No. is 4182, the conservation date is September 16, 2010 and the conservation unit is the China General Microbiological Culture Collection Center. The Bifidobactrium amimalis Subsp Lactis B174 can colonize in human body and resist the gastric acid and cholate of human body. Meanwhile, the strain has better inhibitory action on Escherichia coli and can survive for 30 days at the room temperature. The strain and other lactic acid bacteria have better synergic action for growth; the strain has better combination effect with lactic acid bacteria; the product has better flavor, can be used to prepare fermented yoghurt, animal feed additives and antibacterial solution and can be widely used in the fields of other foods and biological products.

A method and apparatus are provided for performing capillary isoelectric focusing followed by mobilization of the focused zones by induced hydrodynamic flow or chemical mobilization. These two dimensions of separation are integrated with real-time whole-channel electrophoresis detection and automatic sample injection to achieve a separation resolution superior to that obtainable using known orthogonal capillary two dimensional arrangements.

The invention discloses a full-automatic control multifunctional solid-state fermentation tank, comprising a round vertical fermentation tank main body and a fermentation control system, wherein a loading auger is arranged at one side of the upper end of the fermentation tank main body; multiple clapboards are arranged in the fermentation tank main body; a high-material-bed material level automatic control device is arranged in each fermentation layer; a sterile air distribution system is arranged in each fermentation layer and is connected with a sterile air generation tank positioned at the outer side of the fermentation tank main body; a main shaft is rotationally arranged in the fermentation tank main body; and the upper ends of the clapboards are provided with stirring blades. The full-automatic control multifunctional solid-state fermentation tank disclosed by the invention integrates eight functions including dry material mixing, watering material mixing, steam sterilization, inoculation, automatic fermentation control, low-temperature drying, raw material crushing, automatic discharging and finished product smashing, and enables all operations of solid-state fermentation to be completed in a multifunctional biological solid-state fermentation tank; and by virtue of the equipment disclosed by the invention, the fermentation and production preparation processes of solid biological products can be continuously completed.

The invention provides a dry powder composition for a zooblast culture medium. Compared with the prior dry powder composition for the zooblast culture medium, the dry powder composition provided by the invention reduces the serum dosage of animals by introduction of recombinant protein or animal origin compositions, the dry powder composition for the zooblast culture medium can be matched with low-content animal serum for use without additional introduction of protein compositions (such as the recombinant protein, plant protein or the animal origin compositions including cytokines and so on), and has the same or better function on promoting the growth of zooblast compared with high-content animal serum, so that the dry powder composition for the zooblast culture medium does not generate side effects along with the protein compositions, and has good safety and low cost. Cells cultured by the dry powder composition for the zooblast culture medium have small difference for different batches, low cost and good safety, are favorable for separating downstream products of the cells, and are suitable to be used for virus hosts, expression vectors and so on of biological products and other biological products.

The invention discloses a method for continuously separating and purifying valine in fermentation liquor by using simulated moving bed chromatography, and belongs to the technical field of biological product processing. In the method, a simulated moving bed chromatography separating device suitable for the composition characteristic of valine fermentation liquor is designed, water is taken as a mobile phase, and the previously invented resin special for valine separation (disclosed in the Chinese patent 200910231735.4) is taken as a stationary phase. The method can completely remove impurities of alanine and leucine from the valine fermentation liquor, achieves product purity of over 99.7 percent to meet the medicinal standard, is suitable for continuous production, has higher recovery rate and production strength, and overcomes the defects of low purity, low efficiency, high pollution and the like in the conventional valine separation and purification process.

Disclosed is a process of determining, at a point during shipment, whether the solid phase refrigerant in a shipment is sufficient to preserve the shipment cargo, which is blood or other biological products, for the remaining shipment period, by: monitoring the temperatures encountered to said point and estimating the temperatures likely to be encountered during the remaining shipment period; determining the likelihood that the remaining refrigerant can maintain the shipment cargo within a specified temperature range during the remaining shipment period; and if the risk that the remaining refrigerant cannot maintain the shipment cargo within said range during the remaining shipment period is above a cut-off level, then taking action to preserve the value of the cargo.