101 results about "Protein composition" patented technology

The present disclosure relates to a composition comprising a recombinant botulinum toxin protein with improved stability. The composition according to the present disclosure comprises a sugar alcohol as a stabilizer. More specifically, the present disclosure provides a composition comprising a botulinum toxin type A light chain protein or a functional fragment thereof, and comprising, as a stabilizer, a sugar alcohol having six or fewer carbon atoms and / or a solubility in water of 150 mg / mL or more at 20°C. The composition of the present invention can achieve the effect of maintaining the biological activity of the recombinant botulinum toxin light chain protein at a usable level while sufficiently ensuring the stability of the botulinum toxin protein through the stabilizer.

The present application relates to the technical field of health care products, in particular to a pelvic floor muscle repairing composition containing supermolecular tightening Dropsordry and a preparation method thereof, which comprises the following components in mass fraction: 10-14 parts of protein composition, 20-24 parts of chitosan modified Dropsordry, 5-7 parts of freeze-dried powder of Lactobacillus bulgaricus, 10-12 parts of hyaluronic acid, 6-8 parts of glycine and 15-17 parts of proline; each part of the protein composition comprises 20-30 parts of myosin, 30-34 parts of actin, 5-10 parts of hydrolyzed collagen and 240-280 parts of deionized water. The pelvic floor muscle repairing composition can reduce drug toxicity, reduce the amount of drug administration, continuously supplement the required elements of the body during treatment, and quickly relieve the constipation problem caused by the functional damage of the pelvic floor muscle.

The application provides a protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer and application thereof, and belongs to the technical field of prognosis evaluation.The protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer comprises protein molecules for evaluating drug resistance of a patient and protein molecules for evaluating sensitivity of the patient; the protein molecules for evaluating drug resistance of the patient comprise MCM4, NT5E, LTBP4, IAH1, HP, DOCK2 and EEF1A2; and the protein molecules for evaluating sensitivity of the patient comprise TFF3, FABP5 and LAMP2.The application constructs a molecular typing of mHSPC based on the above protein composition, evaluates the sensitivity of a mHSPC patient to ADT treatment in advance, and thus realizes precise treatment.

Disclosed herein is a novel tool to associate protein cargo molecules to the inside and / or outside of a Hepatitis B Virus (HBV) virus-like particle (VLP). The novel tool being composed of a sulfamoylbenzamide (SBA) antiviral, linker, and protein.

The invention relates to the technical field of biosystem molecular probes, in particular to a lipid droplet protein in-situ labeling probe as well as a preparation method and application thereof. The structure of the probe is shown in the specification. The synthesis method of the probe is simple and easy to implement, the probe can be specifically positioned at the lipophilic end of the phospholipid monomolecular layer of the lipid droplet and the lipid center area, and the probe can be used for in-situ and real-time marking of lipid droplet protein in living cells and animal bodies. According to the technology, the in-situ analysis technology of the lipid droplet protein from a cell level to an animal level is realized for the first time, a powerful tool is provided for systematically analyzing the protein composition and the dynamic evolution rule of the lipid droplet, and the technology has a wide application prospect.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

This invention relates to the field of molecular probe technology for biological systems, and particularly to an in-situ labeling probe for lipid droplet proteins, its preparation method, and its applications. The probe of this invention has the following structure: [structure omitted]. The synthesis method of this probe is simple and easy to implement, and it can specifically locate the lipophilic ends and lipid center regions of the phospholipid monolayer of lipid droplets. It can be used for in-situ, real-time labeling of lipid droplet proteins in living cells and animals. This technology represents the first significant leap from cellular to animal-level in-situ analysis of lipid droplet proteins, providing a powerful tool for systematically elucidating the protein composition and dynamic evolution of lipid droplets, and has broad application prospects.

The invention belongs to the technical field of foods, health-care foods and medicines, and particularly relates to a low-triester and low-sterol egg yolk phospholipoprotein composition and a preparation method thereof. The invention provides a low-triester and low-sterol egg yolk phospholipoprotein composition. The content of egg yolk phospholipids is greater than or equal to 40%. Furthermore, the content of the protein is 50%-60%. Correspondingly, the invention also provides a preparation method of the low-triester and low-sterol egg yolk phospholipoprotein composition, and the preparation method comprises the following steps: preparing the egg yolk phospholipoprotein composition by taking egg yolk powder as a raw material and adopting a supercritical CO2 extraction method; the supercritical CO2 extraction method comprises gradient boosting extraction; an entrainer is adopted in the gradient boosting extraction. According to the method, the extraction pressure is only 15-30 MPa, the CO2 flow rate is 20-30 kg / h, and the extraction pressure and the CO2 flow rate are far lower than those in the prior art, so that the overall energy consumption is reduced, and the operation cost is remarkably reduced; and efficient removal of TG and cholesterol is realized.

The application discloses application of a protein and related biomaterials thereof in regulating soybean plant type and improving yield. The application belongs to the technical field of biotechnology, and particularly relates to application of the protein and related biomaterials thereof in regulating soybean plant type and improving yield. The protein of the application is a composition of proteins with amino acid sequences of SEQ ID No. 1 and / or SEQ ID No. 4; or a protein with a function of regulating plant type or / and yield, which is obtained by substitution, deletion and / or addition of amino acid residues on the above-mentioned protein; or a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the above-mentioned protein. By regulating the activity or content of the above-mentioned protein, a soybean variety with changed plant type and improved yield can be obtained.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

In some embodiments, compact protein compositions, such as compact nucleic acid base editors that can be used in gene editing technologies, are disclosed herein.

The invention provides a bivalent bovine viral diarrhea virus antigen protein composition, a subunit vaccine as well as a preparation method and application of the bivalent bovine viral diarrhea virus antigen protein composition and the subunit vaccine, and belongs to the technical field of genetic engineering vaccines. The bivalent bovine viral diarrhea virus antigen protein composition is prepared from a recombinant protein kozak-MLTsp-BVDV1-E2-His and a recombinant protein kozak-IgKsp-BVDV2-E2-His, the amino acid sequence of the recombinant protein kozak-MLTsp-BVDV1-E2-His is as shown in SEQ ID NO.16, and the amino acid sequence of the recombinant protein kozak-IgKsp-BVDV2-E2-His is as shown in SEQ ID NO.18. The invention further discloses a preparation method of the bivalent bovine viral diarrhea virus antigen protein composition. According to the invention, efficient expression of BVDV-1 and BVDV-2 E2 proteins is realized, high-purity E2 proteins are obtained, the safety and effectiveness of vaccines are ensured, and the antibody level of the vaccines is improved.

Provided herein are methods for preparing a protein composition having a reduced quantity of a recombinant cell byproduct which provides an undesirable color to a protein. The methods involve the use of absorbents or flocculants that capture, trap or isolate the recombinant cell byproduct. The methods also involve oxidation of the recombinant cell byproduct with an oxidizing agent (e.g. hydrogen peroxide) or light (e.g. photochemical oxidation).

The present invention relates to a method for preparing a modified protein composition under conditions in which the free thiol groups of β-lactoglobulin are exposed to and modified by a reaction with an oxidized phenol compound. The resulting modified protein product (e.g., a protein-rich beverage product) has been found to have excellent performance, and in particular, it has been shown to reduce the level of unpleasant odors during sterilization heat treatment at a neutral pH and when such beverage products are consumed.

The invention relates to a low glycemic index composition which comprises the following components in percentage by weight: 10-35% of a protein composition, 20-55% of a fat composition, 5-35% of a saccharide composition, 1-15% of a dietary fiber composition, 1-2% of a trace element composition, 0.1-1.5% of a vitamin composition and 5-20% of a macroelement composition, according to the composition with the low glycemic index, all the components are scientifically compounded, the digestion and absorption speed of carbohydrates is effectively controlled, the blood glucose level of diabetic patients or patients with impaired glucose tolerance is effectively controlled, comprehensive and balanced nutrition is provided for the diabetic patients or the patients with impaired glucose tolerance, and the composition is beneficial for repairing and improving the immunity of the diabetic patients or the patients with impaired glucose tolerance; and the feed also has the advantages of excellent quality uniformity, stability and fluidity, light taste, suitability for tube feeding and the like.

The invention pertains to an extruded protein composition comprising a vegetable protein and a protein-containing composition from an aleurone-containing plant-based material, wherein the protein-containing composition comprises at least 6 wt% fat and at least 30 wt% protein, based on the total dry weight of the protein-containing composition from an aleurone-containing plant-based material.

The present disclosure provides for IL2 engrafted into the CDR sequences of an antibody having preferred therapeutic profiles over molecules known and used in the clinic. In particular, the provided antibody cytokine engrafted protein compositions increase or maintain CD8+ T effector cells while reducing the activity of Treg cells. Additionally, provided compositions convey improved half-life, stability and produceability over recombinant human IL2 formulations such as Proleukin®.

The present application relates to the technical field of biological medicine, in particular to a diplommatin composition for regulating ovary function and improving menopausal syndrome, a preparation method and application thereof, active ingredients of the composition include diplommatin and cranberry extract by mass fraction, and the mass ratio of diplommatin to cranberry extract is 1:(0.5-5), or active ingredients of the composition are composed of diplommatin, cranberry extract and carob extract according to a mass ratio of 1:(0.5-5):(0.5-5). The composition has a simple component design, effectively solves the problems of "complex components, unknown mechanism, low bioavailability and poor long-term safety" in the prior art through multi-component synergy, and precisely regulates ovary function and systematically improves menopausal syndrome related symptoms.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of one or more of ash, metal salts, alkaloids, particulates, heavy metals, and other impurities and / or contaminants from extracts, as well as modifying the sensory characteristics (e.g., odor, color, and / or taste characteristics) of extracts. The methods generally include diafiltration, treatment with functionalized resins, and supercritical extraction. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by one or more of: an ash content of less than about 15% by weight; a nicotine content of less than about 10 μg / g; and a heavy metal content of less than about 60 μg / g.

The application discloses a silk protein composition for hair growth, and belongs to the technical field of hair growth silk protein compositions. The composition comprises moisturizing agents, fragrance imparting agents, cationic surfactants, acidity adjusting agents, co-emulsifiers, preservatives, antioxidants, oily ingredients, trace ingredients, lauroylsarcosine sodium, cocoyl glutamic acid TEA salt, sodium lauroamphoacetate, sodium chloride, phenoxyethanol, sodium laurate, citric acid, allantoin, cocamide propyl betaine, hydroxybenzoic acid ester, nicotinamide and a silk protein solution. The application has the beneficial effect that the product contains proteins and amino acids, can supplement the required nutrients for damaged and fragile hair, can stimulate the cell proliferation of human head hair papilla cells and hair matrix cells, can make the head of people with thinning hair or baldness regenerate hair, and can prevent hair loss for people with normal hair.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The present disclosure provides novel mirror image CRISPR compositions that include components such as mirror image CRISPR-associated endonucleases and mirror image guide RNA for processing of mirror image nucleic acids. In some embodiments, the composition is a mirror image DNA-cleaving and / or modifying composition including a D-form DNA endonuclease (e.g., a D-form Cas protein) and a L-form guide RNA that includes a sequence complementary to a target sequence of a target L-DNA. In some embodiments, the composition further comprises a D-form DNA ligase (e.g., a D-form T4 ligase protein). Also provided are D-form Cas protein and D-form T4 ligase protein compositions, synthetic precursors thereof, and methods of preparing the same.

The invention provides a flour product and a preparation method thereof, the flour product comprises flour, whole egg liquid and soybean protein isolate, the total weight of the whole egg liquid and the soybean protein isolate accounts for 0.977%-10.9% of the weight of the flour, the weight ratio of the whole egg liquid to the soybean protein isolate is (10-30): 2.7, and the total weight of the whole egg liquid and the soybean protein isolate accounts for 0.977%-10.9% of the weight of the flour. The water-holding capacity of the soybean protein isolate is 4 g / g-7 g / g. According to the present invention, the total amount of the whole egg liquid and the soybean protein isolate is regulated, the weight ratio of the whole egg liquid to the soybean protein isolate and the water-holding capacity of the soybean protein isolate are respectively in the specific range, such that the prepared flour product has the balanced nutrition while the low amount of the egg liquid is added so as to provide the rich nutrition; the color is milky white or creamy yellow, the color is bright, the surface structure is uniform and smooth, the biting force is moderate, the tooth sticking is avoided, the biting force is strong, the elasticity is high, and the taste is smooth.

The invention relates to the field of protein, in particular to a protein composition for detecting ADAMTS13 activity and application of the protein composition. The protein combination for detecting the activity of the ADAMTS13 is composed of two protein subchains, namely, Chain A and Chain B. The Chain A comprises annularly arranged GFP as shown in SEQ ID NO.1, VPart1 of a vWF variant as shown in SEQ ID NO.2 and negative chain polypeptide as shown in SEQ ID NO.5, and the Chain B comprises GFP as shown in SEQ ID NO.1, VPart1 of the vWF variant as shown in SEQ ID NO.2 and negative chain polypeptide as shown in SEQ ID NO.5. The Chain B comprises a nano antibody as shown in SEQ ID NO.6, a V Part2 of a vWF (D1596-R1668) variant as shown in SEQ ID NO.3, and a positive chain polypeptide as shown in SEQ ID NO.4. The invention also discloses a preparation method of the chain B. The protein combination for detecting the activity of the ADAMTS13 can be used for directly determining the activity of the ADAMTS13 enzyme in a blood sample, a biological sample or a chemical sample, and is suitable for clinical instant inspection, biological sample screening and drug research and development scenes.

It essentially has three compartments separated by a partition. By filling each compartment with food and eating from them, one can maintain their health without needing any special expertise or significant effort. This provides a container for a balanced and healthy diet that is easy to maintain without giving up. [Solution] Partitions 4a, 4b, and 4c are provided to separate areas where foods containing various nutrients are stored or placed, such as foods mainly composed of carbohydrates, foods mainly composed of protein, and foods mainly composed of vegetables, mushrooms, and seaweed.

The invention relates to a protein composition for terahertz electromagnetic wave stress culture as well as a preparation method, a detection method and application of the protein composition. According to the invention, the frequency and stress time of terahertz electromagnetic waves are scientifically selected, and the protein composition obtained by culturing the stress stem cells has good cell oxidative damage repair and nerve damage repair effects, can be used for treating nerve cell function change or inflammation related diseases, and has a remarkable effect.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming a plant, plant tissue, plant part, plant cell, and plant seed with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The present invention relates to a composition for DNA sequence analysis and a method for DNA sequence analysis, the method comprising treating a sample with the composition. The composition of the present invention can attain efficient optical identification at a single-DNA molecule level by linking both an A / T-specific DNA-binder agent and an A / T-non-specific complementary DNA-binder agent to DNA, and thus can be helpfully used in studying chromosomal organization of genomes, protein immunolocalization, and the like.

Disclosed are useful compositions of mixed lineage kinase domain-like (MLKL) derived fusion constructs and their methods of use in cellular depletion therapies. Embodiments of the present disclosure provide a chimeric cell death protein that induces immunogenic cell death in various types of cancers and also has applications in viral infection. The present disclosure also provides different methods of delivering the disclosed compositions or fusion constructs to a subject with cancer or tumor.

The present invention provides a means for efficiently producing natural rubber and achieving stable securing, with the aim of securing rubber resources. The present invention provides a protein composition comprising (A-1) a protein having the same activity as CPT6 or (A-2) a protein having the same activity as CPT7 and comprising (B) a protein having the same activity as CPTL, a lipid membrane construct comprising the above composition, a method for producing the same, a cell expressing the proteins constituting the above composition, a method for producing the same, and a method for producing an isoprene polymer compound using any one of these.