156 results about "Protein composition" patented technology

The present disclosure relates to a composition comprising a recombinant botulinum toxin protein with improved stability. The composition according to the present disclosure comprises a sugar alcohol as a stabilizer. More specifically, the present disclosure provides a composition comprising a botulinum toxin type A light chain protein or a functional fragment thereof, and comprising, as a stabilizer, a sugar alcohol having six or fewer carbon atoms and / or a solubility in water of 150 mg / mL or more at 20°C. The composition of the present invention can achieve the effect of maintaining the biological activity of the recombinant botulinum toxin light chain protein at a usable level while sufficiently ensuring the stability of the botulinum toxin protein through the stabilizer.

Methods of determining the stoichiometry of a viral capsid and / or determining the heterogeneity of protein components in a viral capsid are described.

The invention discloses an auxiliary protein composition, iron oxide nanoparticles, a preparation method and application, the composition comprises an SC protein with an amino acid sequence as shown in SEQ ID NO: 1 and an ST protein with an amino acid sequence as shown in SEQ ID NO: 2, and the composition can be used for modifying the iron oxide nanoparticles. The auxiliary protein composition can effectively promote endocytosis of the iron oxide nanoparticles, and assists the iron oxide nanoparticles to realize lysosome escape; moreover, the iron oxide nanoparticles modified by the auxiliary protein composition are good in biocompatibility and long in retention and storage time in cells, can be used for long-time labeling of the cells, and have wide clinical transformation and application prospects as a cell tracer agent.

The present application relates to the technical field of health care products, in particular to a pelvic floor muscle repairing composition containing supermolecular tightening Dropsordry and a preparation method thereof, which comprises the following components in mass fraction: 10-14 parts of protein composition, 20-24 parts of chitosan modified Dropsordry, 5-7 parts of freeze-dried powder of Lactobacillus bulgaricus, 10-12 parts of hyaluronic acid, 6-8 parts of glycine and 15-17 parts of proline; each part of the protein composition comprises 20-30 parts of myosin, 30-34 parts of actin, 5-10 parts of hydrolyzed collagen and 240-280 parts of deionized water. The pelvic floor muscle repairing composition can reduce drug toxicity, reduce the amount of drug administration, continuously supplement the required elements of the body during treatment, and quickly relieve the constipation problem caused by the functional damage of the pelvic floor muscle.

This invention relates to the field of protein composition analysis technology, and particularly to a method for protein composition analysis of succinyl gelatin injection solution, comprising: preparing a mobile phase and several reagents; pretreating several succinyl gelatin injection solution samples to obtain several peptide fragments; setting chromatographic and mass spectrometric conditions; separating the peptide fragments by chromatography and eluting them according to an elution program; acquiring data of the eluted peptide fragments using a mass spectrometer to obtain primary and secondary mass spectra; comparing the acquired mass spectra with a database using software to identify the protein composition in the samples; determining the pass / fail status of the identification process based on a protein component consistency index, and, based on non-compliance conditions, increasing the number of samples according to the difference between the preset consistency index and the protein component consistency index. This invention improves the accuracy of protein composition analysis.

The application provides a protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer and application thereof, and belongs to the technical field of prognosis evaluation.The protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer comprises protein molecules for evaluating drug resistance of a patient and protein molecules for evaluating sensitivity of the patient; the protein molecules for evaluating drug resistance of the patient comprise MCM4, NT5E, LTBP4, IAH1, HP, DOCK2 and EEF1A2; and the protein molecules for evaluating sensitivity of the patient comprise TFF3, FABP5 and LAMP2.The application constructs a molecular typing of mHSPC based on the above protein composition, evaluates the sensitivity of a mHSPC patient to ADT treatment in advance, and thus realizes precise treatment.

The present application belongs to the technical field of protein composition, and relates to a collagen composition and a preparation method thereof. The present application provides a preparation method of a collagen composition, which comprises the following steps: preparing a collagen solution by treating raw hide which has not been subjected to chemical cross-linking treatment; adding a cross-linking promoter, an isothiocyanate and alpha-mammeigin to the collagen solution; and finally obtaining the collagen composition by multiple short-time microwave treatment. The collagen composition provided by the present application avoids the use of a cross-linking agent, has the advantages of no cytotoxicity, high cross-linking degree and good stability, and exhibits excellent antibacterial and antioxidant functions.

Disclosed herein is a novel tool to associate protein cargo molecules to the inside and / or outside of a Hepatitis B Virus (HBV) virus-like particle (VLP). The novel tool being composed of a sulfamoylbenzamide (SBA) antiviral, linker, and protein.

It is an object of the present invention to provide an RpBphP1 mutant and an RpPpsR2 mutant that have been improved so that their binding and dissociation can be reliably controlled by light irradiation with a near-infrared light and blocking thereof. More specifically, the present invention relates to a protein set consisting of two proteins that bind to each other under near-infrared light irradiation, the protein set being a set of an RpBphP1 mutant and a wild-type RpPpsR2, a set of a wild-type RpBphP1 and an RpPpsR2 mutant, or a set of an RpBphP1 mutant and an RpPpsR2 mutant, wherein the binding of the protein set is weaker under the dark and / or is stronger under near-infrared light irradiation, than the binding of a wild-type RpBphP1 and a wild-type RpPpsR2.

The invention relates to the field of nucleic acid editing. In particular, the present invention relates to compositions comprising a first nucleic acid and a second nucleic acid encoding a Cas effector protein and a guide RNA, respectively, as well as vectors and host cells comprising said compositions. The invention also relates to complexes for nucleic acid editing (e.g., gene or genome editing), and methods of using the compositions and complexes for nucleic acid editing (e.g., gene or genome editing).

The invention relates to the technical field of biosystem molecular probes, in particular to a lipid droplet protein in-situ labeling probe as well as a preparation method and application thereof. The structure of the probe is shown in the specification. The synthesis method of the probe is simple and easy to implement, the probe can be specifically positioned at the lipophilic end of the phospholipid monomolecular layer of the lipid droplet and the lipid center area, and the probe can be used for in-situ and real-time marking of lipid droplet protein in living cells and animal bodies. According to the technology, the in-situ analysis technology of the lipid droplet protein from a cell level to an animal level is realized for the first time, a powerful tool is provided for systematically analyzing the protein composition and the dynamic evolution rule of the lipid droplet, and the technology has a wide application prospect.

The present invention relates to a method of preparing a modified protein composition by reaction with an oxidized phenolic compound under conditions of exposure and modification of the free thiol groups of beta-lactoglobulin. The resulting modified protein products have been found to have excellent performance in, for example, protein-rich beverage products, and have particularly been found to produce reduced levels of unpleasant odors during neutral pH sterilization heat treatments as well as during consumption of such beverage products.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

The application discloses a MAPK related protein for regulating flowering and fertility of plants, and belongs to the field of botany, and particularly relates to the application of a MAPK related protein for regulating flowering and fertility of plants.A method for regulating flowering and fertility of plants is provided, which comprises regulating the activity and / or content of a protein in a target plant, or / and regulating the expression amount of a gene coding the protein, so as to regulate the flowering and fertility of the plant, wherein the protein is a composition composed of a protein FvMAPK3 with an amino acid sequence of SEQ ID No.3 and a protein FvMAPK6 with an amino acid sequence of SEQ ID No.6.Experiments prove that reducing the expression of the two proteins FvMAPK3 and FvMAPK6 can regulate the flowering and fertility of strawberries, and has important theoretical significance for strawberry breeding.

This invention relates to the field of molecular probe technology for biological systems, and particularly to an in-situ labeling probe for lipid droplet proteins, its preparation method, and its applications. The probe of this invention has the following structure: [structure omitted]. The synthesis method of this probe is simple and easy to implement, and it can specifically locate the lipophilic ends and lipid center regions of the phospholipid monolayer of lipid droplets. It can be used for in-situ, real-time labeling of lipid droplet proteins in living cells and animals. This technology represents the first significant leap from cellular to animal-level in-situ analysis of lipid droplet proteins, providing a powerful tool for systematically elucidating the protein composition and dynamic evolution of lipid droplets, and has broad application prospects.

Plants, plant parts, and a population of plants or plant parts comprising reduced BIG SEEDS (BS) activity, and compositions and methods of producing such plants, plant parts, and a population of plants and plant parts are provided. The plants, plant parts, or population can have a genetic mutation or a modification of the DNA methylation pattern that reduces the BIG SEEDS activity, which can be located at least partially in at least one BS gene or homolog or in its regulatory region. The plants, plant parts, and population can have increased organ size and / or protein or amino acid content. Also provided are seed, protein, and / or oil compositions and food and beverage products produced from the plants or plant parts provided herein.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

The invention belongs to the technical field of foods, health-care foods and medicines, and particularly relates to a low-triester and low-sterol egg yolk phospholipoprotein composition and a preparation method thereof. The invention provides a low-triester and low-sterol egg yolk phospholipoprotein composition. The content of egg yolk phospholipids is greater than or equal to 40%. Furthermore, the content of the protein is 50%-60%. Correspondingly, the invention also provides a preparation method of the low-triester and low-sterol egg yolk phospholipoprotein composition, and the preparation method comprises the following steps: preparing the egg yolk phospholipoprotein composition by taking egg yolk powder as a raw material and adopting a supercritical CO2 extraction method; the supercritical CO2 extraction method comprises gradient boosting extraction; an entrainer is adopted in the gradient boosting extraction. According to the method, the extraction pressure is only 15-30 MPa, the CO2 flow rate is 20-30 kg / h, and the extraction pressure and the CO2 flow rate are far lower than those in the prior art, so that the overall energy consumption is reduced, and the operation cost is remarkably reduced; and efficient removal of TG and cholesterol is realized.

The application discloses application of a protein and related biomaterials thereof in regulating soybean plant type and improving yield. The application belongs to the technical field of biotechnology, and particularly relates to application of the protein and related biomaterials thereof in regulating soybean plant type and improving yield. The protein of the application is a composition of proteins with amino acid sequences of SEQ ID No. 1 and / or SEQ ID No. 4; or a protein with a function of regulating plant type or / and yield, which is obtained by substitution, deletion and / or addition of amino acid residues on the above-mentioned protein; or a fusion protein obtained by connecting a protein tag to the N terminal and / or C terminal of the above-mentioned protein. By regulating the activity or content of the above-mentioned protein, a soybean variety with changed plant type and improved yield can be obtained.

Disclosed herein are transformed plants, plant tissues, plant parts, plant cells, and plant seeds comprising a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity. Also disclosed herein are methods of protecting or treating a plant from infection by a plant pathogen or pest by transforming plants, plant tissues, plant parts, plant cells, and plant seeds with a recombinant nucleic acid molecule encoding a polypeptide having pesticidal activity.

In some embodiments, compact protein compositions, such as compact nucleic acid base editors that can be used in gene editing technologies, are disclosed herein.

The invention provides a bivalent bovine viral diarrhea virus antigen protein composition, a subunit vaccine as well as a preparation method and application of the bivalent bovine viral diarrhea virus antigen protein composition and the subunit vaccine, and belongs to the technical field of genetic engineering vaccines. The bivalent bovine viral diarrhea virus antigen protein composition is prepared from a recombinant protein kozak-MLTsp-BVDV1-E2-His and a recombinant protein kozak-IgKsp-BVDV2-E2-His, the amino acid sequence of the recombinant protein kozak-MLTsp-BVDV1-E2-His is as shown in SEQ ID NO.16, and the amino acid sequence of the recombinant protein kozak-IgKsp-BVDV2-E2-His is as shown in SEQ ID NO.18. The invention further discloses a preparation method of the bivalent bovine viral diarrhea virus antigen protein composition. According to the invention, efficient expression of BVDV-1 and BVDV-2 E2 proteins is realized, high-purity E2 proteins are obtained, the safety and effectiveness of vaccines are ensured, and the antibody level of the vaccines is improved.

The invention relates to the technical field of fermentation, in particular to a protein composition for promoting calcium absorption and muscle synthesis and preparation and application thereof. The protein composition comprises the following components in parts by weight: 0.5-99 parts of yeast protein and 0.5-20 parts of a calcium source, the protein content in the yeast protein is more than or equal to 70%, and the zinc content in the yeast protein is more than or equal to 200mg / kg; in terms of the weight of the yeast protein, the content of serine in the yeast protein is larger than or equal to 50 mg / g, and / or the content of glutamic acid is larger than or equal to 110 mg / g, and / or the content of leucine is larger than or equal to 90 mg / g, and / or the content of isoleucine is larger than or equal to 55 mg / g, and / or the content of valine is larger than or equal to 50 mg / g, and / or the content of lysine is larger than or equal to 94 mg / g. The protein composition prepared by combining the yeast protein as a main raw material with the calcium source and nutritional ingredients such as the vitamin D, the vitamin K, the collagen or the casein phosphopeptides not only can promote absorption and utilization of calcium, but also can promote muscle synthesis and improve muscle quality, so that the requirement of collaborative health of muscles and bones is met.

Provided herein are methods for preparing a protein composition having a reduced quantity of a recombinant cell byproduct which provides an undesirable color to a protein. The methods involve the use of absorbents or flocculants that capture, trap or isolate the recombinant cell byproduct. The methods also involve oxidation of the recombinant cell byproduct with an oxidizing agent (e.g. hydrogen peroxide) or light (e.g. photochemical oxidation).

Disclosed is a protein comprising no more than three human autoantigenic proteins, wherein a first human autoantigenic protein comprises a truncated myelin oligodendrocyte glycoprotein (MOG) amino acid sequence, a second human autoantigenic protein comprises a myelin basic protein (MBP) amino acid sequence, and a third human autoantigenic protein comprises a truncated proteolipid protein (PLP) amino acid sequence. Also disclosed are related nucleic acids, pharmaceutical compositions, methods of treating a demyelinating disease, and methods of producing the proteins.

The present invention relates to a method for preparing a modified protein composition under conditions in which the free thiol groups of β-lactoglobulin are exposed to and modified by a reaction with an oxidized phenol compound. The resulting modified protein product (e.g., a protein-rich beverage product) has been found to have excellent performance, and in particular, it has been shown to reduce the level of unpleasant odors during sterilization heat treatment at a neutral pH and when such beverage products are consumed.

The invention discloses a muscle-building dietary flavone compound protein composition as well as a preparation method and application thereof, and belongs to the field of dietary supplements. The composition is prepared from the following components in parts by weight: 50 to 80 parts of concentrated whey protein powder, 5 to 20 parts of separated whey protein powder, 0.01 to 3 parts of hydrolyzed whey protein powder, 0.1 to 1 part of bovine colostrum powder, 0.01 to 0.05 part of kaempferol and 0.01 to 0.1 part of glucomannan. The composition can improve the muscle synthesis and growth rate, inhibit muscle reduction caused by sarcopenia, improve the muscle oxidation resistance, accelerate the muscle repair speed and activate the protein synthesis rate-limiting enzyme AKT and the growth kinase ERK. The composition can be used for preparing food, special diet and health food.

The invention relates to a low glycemic index composition which comprises the following components in percentage by weight: 10-35% of a protein composition, 20-55% of a fat composition, 5-35% of a saccharide composition, 1-15% of a dietary fiber composition, 1-2% of a trace element composition, 0.1-1.5% of a vitamin composition and 5-20% of a macroelement composition, according to the composition with the low glycemic index, all the components are scientifically compounded, the digestion and absorption speed of carbohydrates is effectively controlled, the blood glucose level of diabetic patients or patients with impaired glucose tolerance is effectively controlled, comprehensive and balanced nutrition is provided for the diabetic patients or the patients with impaired glucose tolerance, and the composition is beneficial for repairing and improving the immunity of the diabetic patients or the patients with impaired glucose tolerance; and the feed also has the advantages of excellent quality uniformity, stability and fluidity, light taste, suitability for tube feeding and the like.

The invention pertains to an extruded protein composition comprising a vegetable protein and a protein-containing composition from an aleurone-containing plant-based material, wherein the protein-containing composition comprises at least 6 wt% fat and at least 30 wt% protein, based on the total dry weight of the protein-containing composition from an aleurone-containing plant-based material.