232 results about "Protein composition" patented technology

The invention belongs to the technical field of food, and particularly relates to a high-satiety liquid protein composition as well as a preparation method and application thereof. The high-satiety liquid protein composition is characterized by being prepared from the following raw materials in parts by weight: 800 to 1000 parts of protein, 110 to 160 parts of vegetable oil, 700 to 800 parts of carbohydrate, 120 to 150 parts of dietary fiber, 5 to 15 parts of vitamin, 1 to 8 parts of mineral substance, 35 to 40 parts of emulsifier, 12 to 15 parts of stabilizer, 17 to 20 parts of pH regulator and 5 to 10 parts of essence. Under the comprehensive action of all the components, the liquid protein composition can keep the milk state stable, fat components are not subjected to lactation separation, protein components are not subjected to sedimentation and layering, the macroscopic state is stable, and nutrients in the product are uniformly distributed.

The present disclosure provides a method of preparing a lyophilized bispecific molecule composition comprising (a) inducing ice nucleation in a liquid protein composition having a bispecific molecule concentration of at least about 10 mg / ml in a vial exposed to a first temperature of about-18 DEG C to about-10 DEG C for about 60 minutes to about 270 minutes; (b) exposing the vial of (a) to a second temperature of about-25 DEG C to-50 DEG C for about 1 hour to about 5 hours; and (c) drying the composition of (b) at a third temperature of about-5 DEG C to about-25 DEG C for about 25 hours to about 70 hours, and (d) further drying the composition of (c) at a fourth temperature of about 25 DEG C to about 50 DEG C for about 4 hours to about 12 hours to obtain a vial comprising a lyophilized bispecific molecular composition, the composition has a percentage content of high molecular weight material (HMWS) of less than or equal to about 1.5% (mA / ). The disclosure further provides lyophilized bispecific molecule compositions prepared via the methods described herein.

The present invention provides a botulinum toxin protein composition, mainly comprising botulinum toxin protein, a saccharide, a salt, and human serum albumin. The botulinum toxin protein composition of the present invention features good activity and stable properties, and can maintain the activity of the botulinum toxin protein at a high level after long-term storage.

The present invention relates to a method to increase the physical stability of a β-lactoglobulin and to a β-lactoglobulin with increased physical stability. The present invention further relates to the use of an extraneous β-lactoglobulin to enhance the physical stability of a composition comprising a protein and the extraneous β-lactoglobulin.

The present disclosure relates to a composition comprising a recombinant botulinum toxin protein with improved stability. The composition according to the present disclosure comprises a sugar alcohol as a stabilizer. More specifically, the present disclosure provides a composition comprising a botulinum toxin type A light chain protein or a functional fragment thereof, and comprising, as a stabilizer, a sugar alcohol having six or fewer carbon atoms and / or a solubility in water of 150 mg / mL or more at 20°C. The composition of the present invention can achieve the effect of maintaining the biological activity of the recombinant botulinum toxin light chain protein at a usable level while sufficiently ensuring the stability of the botulinum toxin protein through the stabilizer.

Methods of determining the stoichiometry of a viral capsid and / or determining the heterogeneity of protein components in a viral capsid are described.

The invention discloses an auxiliary protein composition, iron oxide nanoparticles, a preparation method and application, the composition comprises an SC protein with an amino acid sequence as shown in SEQ ID NO: 1 and an ST protein with an amino acid sequence as shown in SEQ ID NO: 2, and the composition can be used for modifying the iron oxide nanoparticles. The auxiliary protein composition can effectively promote endocytosis of the iron oxide nanoparticles, and assists the iron oxide nanoparticles to realize lysosome escape; moreover, the iron oxide nanoparticles modified by the auxiliary protein composition are good in biocompatibility and long in retention and storage time in cells, can be used for long-time labeling of the cells, and have wide clinical transformation and application prospects as a cell tracer agent.

The present invention is in the field of protein compositions, in particular protein compositions for infant milk formula. The protein compositions according to the invention comprise whey protein, casein and high isoelectric point proteins, such as those having an isoelectric point of at least 6.0. The present invention further relates to the uses of the protein compositions.

The present application relates to the technical field of health care products, in particular to a pelvic floor muscle repairing composition containing supermolecular tightening Dropsordry and a preparation method thereof, which comprises the following components in mass fraction: 10-14 parts of protein composition, 20-24 parts of chitosan modified Dropsordry, 5-7 parts of freeze-dried powder of Lactobacillus bulgaricus, 10-12 parts of hyaluronic acid, 6-8 parts of glycine and 15-17 parts of proline; each part of the protein composition comprises 20-30 parts of myosin, 30-34 parts of actin, 5-10 parts of hydrolyzed collagen and 240-280 parts of deionized water. The pelvic floor muscle repairing composition can reduce drug toxicity, reduce the amount of drug administration, continuously supplement the required elements of the body during treatment, and quickly relieve the constipation problem caused by the functional damage of the pelvic floor muscle.

This invention relates to the field of protein composition analysis technology, and particularly to a method for protein composition analysis of succinyl gelatin injection solution, comprising: preparing a mobile phase and several reagents; pretreating several succinyl gelatin injection solution samples to obtain several peptide fragments; setting chromatographic and mass spectrometric conditions; separating the peptide fragments by chromatography and eluting them according to an elution program; acquiring data of the eluted peptide fragments using a mass spectrometer to obtain primary and secondary mass spectra; comparing the acquired mass spectra with a database using software to identify the protein composition in the samples; determining the pass / fail status of the identification process based on a protein component consistency index, and, based on non-compliance conditions, increasing the number of samples according to the difference between the preset consistency index and the protein component consistency index. This invention improves the accuracy of protein composition analysis.

The application provides a protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer and application thereof, and belongs to the technical field of prognosis evaluation.The protein composition for predicting the prognosis effect of metastatic hormone-sensitive prostate cancer comprises protein molecules for evaluating drug resistance of a patient and protein molecules for evaluating sensitivity of the patient; the protein molecules for evaluating drug resistance of the patient comprise MCM4, NT5E, LTBP4, IAH1, HP, DOCK2 and EEF1A2; and the protein molecules for evaluating sensitivity of the patient comprise TFF3, FABP5 and LAMP2.The application constructs a molecular typing of mHSPC based on the above protein composition, evaluates the sensitivity of a mHSPC patient to ADT treatment in advance, and thus realizes precise treatment.

To provide methods for producing aflibercept with reduced amount of aflibercept variants.SOLUTION: Disclosed is a method for producing aflibercept with a reduced amount of aflibercept variants expressed in a host cell cultured in a chemically defined medium (CDM), where the target value of aflibercept variants can be obtained when the cumulative concentration of iron in the CDM is about 55.0 μM or less, the cumulative concentration of copper in the CDM is about 0.8 μM or less, the cumulative concentration of nickel in the CDM is about 0.4 μM or less, the cumulative concentration of zinc in the CDM is about 56.0 μM or less, the cumulative concentration of cysteine in the CDM is about 10.0 mM or less, and / or when any single anti-oxidant is added in the CDM the cumulative concentration is about 0.001 mM to about 10 mM, and when a plurality of anti-oxidants are added the cumulative concentration of total antioxidants is about 30 mM or less.SELECTED DRAWING: Figure 1

The invention discloses a TIR-STAND anti-phage, an anti-virus system and application of the TIR-STAND anti-phage and the anti-virus system and relates to the technical field of biology. The protein composition provided by the invention is composed of three structural domains of TIR, NTPase and TPR; nucleic acid molecules, recombinant expression vectors, and host cells expressing the protein compositions are provided; the invention also provides an application of the protein composition in bacteriophage resistance or virus resistance. According to the invention, the knowledge of the existing immune system for resisting bacteriophage is expanded, and a new thought and application are provided for the development of biotechnology, the prevention and treatment of clinical pathogenic bacteria and the anti-bacteriophage pollution in the fermentation process.

The present application belongs to the technical field of protein composition, and relates to a collagen composition and a preparation method thereof. The present application provides a preparation method of a collagen composition, which comprises the following steps: preparing a collagen solution by treating raw hide which has not been subjected to chemical cross-linking treatment; adding a cross-linking promoter, an isothiocyanate and alpha-mammeigin to the collagen solution; and finally obtaining the collagen composition by multiple short-time microwave treatment. The collagen composition provided by the present application avoids the use of a cross-linking agent, has the advantages of no cytotoxicity, high cross-linking degree and good stability, and exhibits excellent antibacterial and antioxidant functions.

Disclosed herein is a novel tool to associate protein cargo molecules to the inside and / or outside of a Hepatitis B Virus (HBV) virus-like particle (VLP). The novel tool being composed of a sulfamoylbenzamide (SBA) antiviral, linker, and protein.

The invention provides a preparation method of a cooling agent-containing mouth mask for cigarettes as well as a product and application thereof. The preparation method comprises the following steps: (1) mixing polysaccharide, a cooling agent and an oxalic acid aqueous solution to obtain a mixed solution; mixing the protein composition with water to obtain a protein solution; and (2) mixing the mixed solution, the protein solution and an auxiliary agent, carrying out ultrasonic degassing treatment, putting into a mold, and drying to obtain the cooling agent-containing mouth film for cigarettes. According to the preparation method disclosed by the invention, through the matching of specific raw materials, the film-forming property of the polysaccharide, the flexibility after film formation and other mechanical properties are improved, the protein loading capacity of the polysaccharide is improved, and the cooling agent is added, so that the final mouth film not only has excellent mechanical properties, but also can provide a cool feeling for cigarettes after being used for the cigarettes.

The invention belongs to the technical field of biological medicine and skin repair, and particularly relates to a recombinant extracellular matrix protein composition and application thereof in preparation of products for resisting skin photoaging. The recombinant extracellular matrix protein composition is obtained by mixing recombinant humanized III collagen, recombinant humanized chemical fibronectin and recombinant humanized elastin. Tests show that the recombinant extracellular matrix protein composition can improve the survival rate of cells damaged by UVB, reduce the generation of ROS, improve the activity of SOD, CAT and GSH-Px and the level of GSH, and reduce the content of lipid peroxide MDA. The recombinant extracellular matrix protein composition has an effective anti-oxidative damage effect and can resist skin cell damage caused by UVB.

To provide a combination of proteins that contains a TlpA mutant and can induce substance interaction by heating.SOLUTION: A protein set for inducing substance interaction in a temperature-dependent manner comprises: a protein set comprising a protein having a binding-inducing peptide bound to the N-terminus or C-terminus of the following protein a, the following protein b, and the binding-inducing protein; or a protein set comprising a protein having a binding-inducing peptide bound to the N-terminus or C-terminus of the following protein b, the following protein a, and the binding-inducing protein; a: a protein comprising the whole or a part of a coiled-coil region of a protein having a specific amino acid sequence, and having mutations indicated by E180R and E250R; and b: a protein comprising the whole or a part of a coiled-coil region of a protein having the specific amino acid sequence and having mutations indicated by R179E and R251E.SELECTED DRAWING: None

It is an object of the present invention to provide an RpBphP1 mutant and an RpPpsR2 mutant that have been improved so that their binding and dissociation can be reliably controlled by light irradiation with a near-infrared light and blocking thereof. More specifically, the present invention relates to a protein set consisting of two proteins that bind to each other under near-infrared light irradiation, the protein set being a set of an RpBphP1 mutant and a wild-type RpPpsR2, a set of a wild-type RpBphP1 and an RpPpsR2 mutant, or a set of an RpBphP1 mutant and an RpPpsR2 mutant, wherein the binding of the protein set is weaker under the dark and / or is stronger under near-infrared light irradiation, than the binding of a wild-type RpBphP1 and a wild-type RpPpsR2.

The invention relates to the field of nucleic acid editing. In particular, the present invention relates to compositions comprising a first nucleic acid and a second nucleic acid encoding a Cas effector protein and a guide RNA, respectively, as well as vectors and host cells comprising said compositions. The invention also relates to complexes for nucleic acid editing (e.g., gene or genome editing), and methods of using the compositions and complexes for nucleic acid editing (e.g., gene or genome editing).

Disclosed herein are cell culture media comprising a plant protein composition, plant protein compositions, methods of preparing plant protein compositions, cell culture media supplements, and method of using the same, e.g., methods of expanding or differentiating cells. The methods of preparing the protein compositions comprise substantially removing globulins in the plant protein composition, e.g., by reducing the pH of and / or filtering the plant protein composition. The resulting plant protein compositions are used to generate increased yield in cell culture while also maintaining cell growth utility. The plant protein compositions may be used in serum-free culture media and methods of using serum-free culture media, including for expansion of cells.

The invention relates to the technical field of biosystem molecular probes, in particular to a lipid droplet protein in-situ labeling probe as well as a preparation method and application thereof. The structure of the probe is shown in the specification. The synthesis method of the probe is simple and easy to implement, the probe can be specifically positioned at the lipophilic end of the phospholipid monomolecular layer of the lipid droplet and the lipid center area, and the probe can be used for in-situ and real-time marking of lipid droplet protein in living cells and animal bodies. According to the technology, the in-situ analysis technology of the lipid droplet protein from a cell level to an animal level is realized for the first time, a powerful tool is provided for systematically analyzing the protein composition and the dynamic evolution rule of the lipid droplet, and the technology has a wide application prospect.

The present invention relates to a method of preparing a modified protein composition by reaction with an oxidized phenolic compound under conditions of exposure and modification of the free thiol groups of beta-lactoglobulin. The resulting modified protein products have been found to have excellent performance in, for example, protein-rich beverage products, and have particularly been found to produce reduced levels of unpleasant odors during neutral pH sterilization heat treatments as well as during consumption of such beverage products.

Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha / beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and / or white flake protein content.

The application discloses a MAPK related protein for regulating flowering and fertility of plants, and belongs to the field of botany, and particularly relates to the application of a MAPK related protein for regulating flowering and fertility of plants.A method for regulating flowering and fertility of plants is provided, which comprises regulating the activity and / or content of a protein in a target plant, or / and regulating the expression amount of a gene coding the protein, so as to regulate the flowering and fertility of the plant, wherein the protein is a composition composed of a protein FvMAPK3 with an amino acid sequence of SEQ ID No.3 and a protein FvMAPK6 with an amino acid sequence of SEQ ID No.6.Experiments prove that reducing the expression of the two proteins FvMAPK3 and FvMAPK6 can regulate the flowering and fertility of strawberries, and has important theoretical significance for strawberry breeding.

This invention relates to the field of molecular probe technology for biological systems, and particularly to an in-situ labeling probe for lipid droplet proteins, its preparation method, and its applications. The probe of this invention has the following structure: [structure omitted]. The synthesis method of this probe is simple and easy to implement, and it can specifically locate the lipophilic ends and lipid center regions of the phospholipid monolayer of lipid droplets. It can be used for in-situ, real-time labeling of lipid droplet proteins in living cells and animals. This technology represents the first significant leap from cellular to animal-level in-situ analysis of lipid droplet proteins, providing a powerful tool for systematically elucidating the protein composition and dynamic evolution of lipid droplets, and has broad application prospects.

The invention relates to the technical field of solid beverages, in particular to a sesame and soybean protein compound solid beverage, and solves the problems that sesame residue protein in the prior art is low in solubility due to particularity of molecular structures and properties, is too strong in unique flavor, is low in lysine content, and is poor in taste and the like. The problems that the soybean protein is not widely applied to liquid food such as beverages, the flavor of the soybean protein is relatively single and lacks changes, and sulfur-containing amino acid in the protein composition is relatively lacked are solved. A preparation method of the sesame and soybean protein compounded solid beverage comprises the following steps: preparing raw materials, weighing high-solubility sesame protein and soybean protein, accurately weighing according to a ratio of 1: 13, mixing the raw materials, fully and uniformly mixing the weighed sesame protein and soybean protein, and ensuring that the sesame protein and the soybean protein are fully fused. Compared with a commercially available solid beverage, the compound protein solid beverage has the advantages that the Karl index is reduced by 6.75%, and the Hausner ratio is reduced by 0.11, so that the compound protein solid beverage is compact in tissue and easy to brew.

Plants, plant parts, and a population of plants or plant parts comprising reduced BIG SEEDS (BS) activity, and compositions and methods of producing such plants, plant parts, and a population of plants and plant parts are provided. The plants, plant parts, or population can have a genetic mutation or a modification of the DNA methylation pattern that reduces the BIG SEEDS activity, which can be located at least partially in at least one BS gene or homolog or in its regulatory region. The plants, plant parts, and population can have increased organ size and / or protein or amino acid content. Also provided are seed, protein, and / or oil compositions and food and beverage products produced from the plants or plant parts provided herein.