132 results about "Ornithine" patented technology

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to an ornithine decarboxylase mutant as well as a coding gene, an expression vector, a recombinant strain and application thereof. The ornithine decarboxylase mutant is obtained by mutating wild ornithine decarboxylase ODC10 of which the amino acid sequence is shown as SEQ ID NO.1, the catalytic activity of the obtained mutant is improved, and the biosynthesis efficiency of 1, 4-butanediamine is favorably improved. The recombinant strain for expressing the ornithine decarboxylase mutant constructed by the invention can efficiently synthesize 1, 4-butanediamine, and the molar conversion rate of ornithine is remarkably improved, so that the production benefit is improved, and the production cost is reduced.

The invention relates to the field of fermentation processes, in particular to a method for preparing 1, 4-butanediol through multi-strain synergistic fermentation, which comprises the following steps: inoculating a first strain and a second strain into a culture medium containing glucose and / or xylose for synergistic fermentation, producing ornithine by the first strain by taking glucose and / or xylose as a substrate, and converting ornithine into 1, 4-butanediol by the second strain. The invention discloses 1, 4-butanediol. Glucose is converted into ornithine through the first strain, ornithine is converted into 1, 4-butanediol through the second strain, the first strain and the second strain are subjected to synergistic fermentation, preparation of 1, 4-butanediol through microbial synergistic fermentation is achieved, and higher metabolic capacity and higher production efficiency are achieved. The mixed sugar of xylose and glucose prepared from biomass raw materials is used as a substrate for fermentation, so that the method has the advantages that the raw materials are easy to obtain, renewable and low in cost, and the production cost can be reduced.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

The invention discloses a rheumatoid arthritis diagnosis product and system and application thereof, and relates to the technical field of rheumatoid arthritis. The diagnostic marker comprises calcium, a ratio of arginine to ornithine, a ratio of phenylalanine to tyrosine, an anti-cyclic citrullinated peptide antibody and lysine. The invention provides a biomarker capable of accurately diagnosing rheumatoid arthritis RA, which comprises five indexes, namely calcium, the proportion of arginine to ornithine, the proportion of phenylalanine to tyrosine, an anti-cyclic citrullinated peptide antibody (anti-CCP) and lysine. The five indexes have the technical advantages of few indexes and excellent diagnosis performance. A diagnosis model constructed based on the five indexes has good sensitivity, specificity and accuracy. The kit can be used for diagnosis of rheumatoid arthritis.

The invention provides a key enzyme for synthesizing butanediamine and application of the key enzyme, and belongs to the technical field of bioengineering. The invention provides a gene for synthesizing ornithine decarboxylase. The nucleotide sequence of the ornithine decarboxylase is shown as SEQ ID NO. 7. By further optimizing the reaction system, the butanediamine synthesis capability is improved, and butanediamine can be synthesized more efficiently.

The invention relates to the technical field of detection, in particular to a kit for liquid phase detection of amino acid, a pretreatment method and a liquid phase detection method. The kit comprises a protective agent, an activating solution, a leacheate, an eluent and a resolvent, the protective agent comprises a first ammonium acetate solution; the activating solution comprises methanol and a second ammonium acetate solution; the leacheate comprises a formic acid aqueous solution and methanol; the eluent comprises a methanol solution with the pH value of 9 to 13. The detection method using the kit can meet the requirements of related laws and regulations on linearity, repeatability, batch-to-batch difference, accuracy and the like of cystine, lysine, ornithine and arginine, and has important reference significance for evaluating whether cystinuria exists or not.

The invention provides a metabonomics-based preeclampsia risk assessment prediction model construction method, a prediction model, application of metabolites, a metabolite detection kit, a prediction product, a prediction system and related application thereof. Specifically, clinical indexes and metabolite data of a pregnant woman serve as input, whether the pregnant woman suffers from preeclampsia or not serves as output, model construction is conducted through a machine learning method, and a preeclampsia risk assessment and prediction model is obtained. The metabolite is selected from at least one of 4-hydroxyphenyllactic acid, homovanillic acid, ornithine, sarcosine, adenosine monophosphate and 3-methylbutyryl carnitine. According to the invention, not only are six metabolite markers strongly associated with preeclampsia found, but also effective prediction of early preeclampsia and late preeclampsia is realized by establishing a prediction model, and the method has extremely high clinical value.

The invention belongs to the technical field of crop cultivation, and particularly discloses a two-component flavor enhancer for rice flavor enhancement as well as a preparation method and application of the two-component flavor enhancer for rice flavor enhancement, and the two-component flavor enhancer is prepared from a component A and a component B in a weight ratio of 1: (0.9-1.1), wherein the component A is prepared from the following raw materials in parts by weight: 80 to 120 parts of proline and 180 to 220 parts of ornithine; the component B is prepared from 2 to 5 parts of colloidal chitosan nano-selenium and 350 to 450 parts of zinc oxide quantum dots. The flavor enhancer developed in the invention is helpful for efficiently increasing the content of free proline and proline dehydrogenase, increasing the accumulation of 2-AP in rice grains, and effectively improving the rice fragrance.

The present invention provides an objective non-invasive method for examining renal dysfunction in cats. This method for testing renal dysfunction is a method for testing renal dysfunction in cats, and comprises a step for measuring the level of one or more D-amino acids selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-ornithine, and D-histidine in urine of a subject.

The invention belongs to the technical field of biological detection, and particularly relates to a biomarker composition and application thereof in methylation detection of an MGMT promoter. The biomarker composition is prepared from phospholipid (L5PL), creatinine (Creatinine) and ornithine (Ornithine) in the LDL-5 sub-component, and the biomarker composition is prepared from the phospholipid (L5PL), the creatinine (Creatinine) and the ornithine (Ornithine). Whether a glioblastoma patient carries MGMT promoter methylation or not is judged through a plurality of biomarkers, the method has the advantages of being good in sensitivity and specificity, small in patient trauma, capable of overcoming individual differences and the like, and a new solution is provided for prognosis evaluation of glioblastoma.

The present invention discloses Anti-hirsutism compositions, use and methods for managing hirsutism in a subject using the composition comprising turmeric oil standardized to contain Aromatic Turmerone (ar-Turmerone), Eflornithine; Nigella sativa extract; Oroxylum indicum extract; and Licorice extract either individually or combinations thereof. The invention also discloses use of the above composition in inhibiting the activity of ornithine decarboxylase and 5-α-reductase.

The invention discloses a polyarginine nano-particle as well as a preparation method and application thereof. The preparation method comprises the following steps: polymerizing ornithine-carboxylic anhydride (NCA) serving as a monomer with leucine to obtain a diblock polymer, or polymerizing the ornithine-carboxylic anhydride monomer, and then performing guanidination treatment and self-assembly to obtain the polyarginine nanoparticles. The polyarginine nano-particles prepared by the invention can be combined with free nucleic acid released by injured tissues or cells in traumatic brain injury (TBI) to remove the free nucleic acid and prevent downstream immune response reaction, so that the proinflammatory factor level is remarkably reduced, and the purposes of relieving inflammation and treating TBI are achieved; compared with the traditional cationic amino acid (polylysine), the polyarginine material adopted by the invention can effectively improve the prognosis of TBI patients. Therefore, the invention provides the nano material with remarkably improved DNA binding capacity and treatment effect, and the nano material has important significance in treatment of traumatic brain injury.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The present application relates to a recombinant microorganism of the genus Corynebacterium having an L-ornithine producing ability, comprising an amino acid transporter of the LysE / ArgO family derived from a Shewanella strain or a polynucleotide encoding an amino acid transporter of the LysE / ArgO family.

A composition for relieving hangovers and protecting the liver, made from the following raw materials in the indicated weight ratios: 3-20 parts of kudzu root extract, 2-20 parts of Japanese raisin tree fruit extract, 1-12 parts of N-acetylcysteine, 1-12 parts of L-ornithine, 1-15 parts of L-glutamine, 0.2-8 parts of glycyrrhetinic acid glycyrrhiza extract, 0.05-5 parts of curcumin, 0.005-0.5 parts of piperine, 0.5-10 parts of vitamin C, 0.01-2 parts of B vitamins complex, 0.1-8 parts of electrolyte salts, and 1-25 parts of honey or oligosaccharides.

The invention discloses a reagent for enhancing aroma and improving quality of cigar tobacco leaves and application of the reagent, and relates to the technical field of tobacco fertilization and tobacco processing.The reagent comprises an ornithine solution with the mass concentration being 0.1-0.3%, or a glutamic acid solution with the mass concentration being 0.1-0.3%, or a proline solution with the mass concentration being 0.1-0.3%. The cigar tobacco aroma improving method has important significance on improving the quality of cigar tobacco raw materials, and is easy to operate, low in cost, economical and practical.

The invention provides a flora and an application of the flora in biodegradation of polyurethane plastics. The flora is prepared from raoultella ornithinolytica, klebsiella pneumoniae, coxakella rhizogenes and achromobacter xylosoxidans. According to the method, the polyurethane degrading flora is screened from the collected soil sample, the flora can efficiently degrade waterborne polyurethane and thermoplastic polyurethane elastomers with large chemical structure difference at the same time to degrade polyurethane plastic, the process is greatly simplified, the operation cost is reduced, traditional landfill incineration is replaced, green circulation of polyurethane is assisted, and the method has the advantages of being environmentally friendly and environmentally friendly. The method has important practical significance for solving the problem of environmental pollution caused by polyurethane waste and promoting green cyclic utilization of polyurethane materials.

Provided herein are fixed-dose combination formulations of a pharmaceutically effective amount of eflorithine together with a pharmaceutically effective amount of sulindac. Also provided are methods of use and of methods of manufacture of these formulations.

The invention discloses a metabolic biomarker for predicting the progress of hepatic fibrosis and application of the metabolic biomarker, a group of metabolic biomarkers with diagnostic value is screened out through a metabonomics method, and the metabolic biomarkers comprise acetic acid, alanine, arginine, citric acid, glucose, glycerol, lysine, ornithine, pyruvic acid, succinic acid and tyrosine. By using these biomarkers in combination, the accuracy and specificity of diagnosis can be further improved. A biological sample is collected from a tested object, the content of the biomarker is detected, and the content is compared with a preset threshold concentration for analysis, the detection means is nuclear magnetic resonance spectroscopy, and compared with the prior art, the sample pretreatment steps are simpler, the experiment operation is quicker, and the detection accuracy is higher. The problems that early hepatic fibrosis is not easy to perceive and a traditional diagnosis and treatment method is high in cost and long in consumed time are solved, and the accuracy of early hepatic fibrosis diagnosis is effectively improved.

The invention relates to the technical field of plant genetic engineering, and discloses an overexpressed ornithine decarboxylase coding gene, an overexpressed transcription factor coding gene, application of biological materials of the overexpressed ornithine decarboxylase coding gene and the overexpressed transcription factor coding gene, and a method for increasing the nicotine content in tobacco. Meanwhile, the nicotine content in tobacco overexpressed with the two genes is remarkably increased compared with that of a wild type, and is far greater than the increasing amplitude of any one of the genes overexpressed independently, so that the problems that in the prior art, the nicotine content cannot be remarkably increased through gene coordinated regulation and control, and the nicotine increasing amplitude bottleneck is difficult to break through are effectively solved; powerful support is provided for high-nicotine tobacco cultivation.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

Disclosed in the present invention are an atosiban derivative or a pharmaceutically acceptable salt thereof, and the preparation and the use thereof. The structure of the atosiban derivative or the pharmaceutically acceptable salt thereof is as shown in general formula (I) or (II). On the basis of the present invention, fatty acid acylation is performed on an ornithine residue in an atosiban peptide sequence to prepare the derivative, which can effectively improve the antagonistic effect of a parent nucleus. The oxytocin antagonistic effect of the atosiban derivative prepared in the present invention is obviously higher than that of atosiban acetate, and the effect can be effectively used for preventing and / or treating premature delivery when used as an oxytocin antagonist to prepare a drug.

The present invention provides a denitrifying strain capable of removing both toluene and nitrogen under anaerobic conditions and its application, belonging to the field of microbial technology. The denitrifying strain described in the present invention is Raoultella ornithinolytica strain 14, which is deposited in the China Center for Type Culture Collection with a deposit number of CCTCC NO: M 20241685. The Raoultella ornithinolytica strain 14 provided by the present invention can simultaneously achieve the functions of denitrification and toluene removal under anaerobic conditions, is easy to use, simple to operate, reduces the cost of sewage treatment, and has good application prospects in sewage treatment.

The present application relates to a microorganism having the ability to produce L-ornithine and a method for producing L-ornithine using the same.

To provide a novel strain of Tetragenococcus halophilus having superior capability for producing ornithine and citrulline, thereby enabling the efficient production of ornithine and citrulline that are beneficial amino acids, and their application in the manufacture of food compositions, supplements, pharmaceuticals and the like suitable for maintaining or promoting health, including stimulation of growth hormone secretion, regulation of sleep-wake cycles, enhancement of liver function, rejuvenation of fatigued skin, reduction of mental stress, and recovery from physical fatigue.SOLUTION: Provided is a strain of Tetragenococcus halophilus KA12022 (Deposit Number: NITE P-03886).SELECTED DRAWING: Figure 4

The present invention provides an oral composition that suppresses off-flavors derived from ornithine.　Provided is an oral composition containing the following components (A) and (B): (A) 15 mass% to less than 30 mass% of ornithine; and (B) an aromatic ketone. The mass ratio [(B) / (A)] of component (A) and component (B) is 1.7×10-8 to 1.0×10-4.

The invention relates to the technical field of construction of recombinant bacteria, in particular to recombinant corynebacterium glutamicum for producing L-ornithine as well as a construction method and application of the recombinant corynebacterium glutamicum for producing L-ornithine, corynebacterium glutamicum SO30 is taken as an original strain, a SigD gene and a FruR gene are sequentially knocked out through a suicide plasmid pK18mobsacB mediated homologous recombination technology, and double knockout recombinant corynebacterium glutamicum SO30-SigD-FruR is constructed. The L-ornithine yield of the strain subjected to shake-flask fermentation reaches 51.88 g / L and is increased by 56.7% compared with that of an original strain SO30; the fermentation yield of a 5L bioreactor reaches 123 g / L and is increased by 57.6% compared with that of an original strain SO30, and the cell biomass and the sugar conversion rate are remarkably increased. Through global transcriptional regulation factor transformation, the existing yield bottleneck is broken through, and efficient strains and technical support are provided for industrial fermentation production of L-ornithine.

The invention relates to the technical field of metabonomics analysis, in particular to application of a metabolic marker in preparation of a head and neck squamous cell carcinoma diagnostic product, a kit, a screening method of the metabolic marker of the head and neck squamous cell carcinoma, a diagnostic model and a construction method and application of the diagnostic model. The metabolic marker comprises at least one of lactic acid, sphingosine, cadaverine, uridine diphosphate, allantoic acid, hydroxyproline, sphingosine-1-phosphoric acid, indole-3-acetaldehyde, pantothenic acid, fumaric acid, malic acid, prostaglandin or ornithine in red blood cells and / or plasma. The screened red blood cells and plasma metabolism markers can be used for predicting or diagnosing the head and neck squamous cell carcinoma respectively or independently, particularly, the red blood cell metabolism markers can provide more stable tumor microenvironment information, and the metabolism stability is high and is not easily influenced by diet and circadian rhythm.

The invention discloses simulated perspiration and application thereof in preparation of artificial fingerprints, and relates to the technical field of fingerprint development. Comprising amino acids and water, and the amino acids comprise at least three of serine, glycine, alanine, aspartic acid, threonine, histidine, ornithine and valine; the total concentration of amino acids in the simulated sweat is greater than or equal to 3mmol / L. The simulated sweat is obtained by compounding a plurality of amino acids, and the concentration of the amino acids in the simulated sweat is controlled, so that the simulated sweat can better simulate natural fingerprint sediments, the developing effect is achieved, a standard is provided for detecting the developing effect of the developing agent, and the developing capability of the developing agent can be efficiently and accurately evaluated.

The invention discloses a six-membered cyclic ornithine derivative monomer, modified nylon 6 and a preparation method of the six-membered cyclic ornithine derivative monomer, relates to the technical field of polymer synthesis, and solves the problem of application limitation of ornithine in the field of high polymer materials and the problem that preparation of nylon 6 depends on non-renewable resources and is serious in homogenization in the prior art. According to the invention, ornithine is used as a raw material, a series of six-membered cyclic ornithine derivative monomers are prepared through cyclization and upper protection, and the monomers and caprolactam are subjected to ring-opening copolymerization to obtain the modified nylon 6 material. The raw material of the six-membered cyclic ornithine derivative monomer is derived from biomass resource ornithine, the six-membered cyclic ornithine derivative monomer has the characteristics of greenness, environmental protection, energy conservation and low carbon, and the preparation method is simple, high in product yield, low in production cost and suitable for large-scale production; the main chain of the prepared nylon 6 contains renewable ornithine, consumption of non-renewable resources such as petroleum can be reduced, functional side groups can be introduced into the main chain of the nylon 6, and the printing and dyeing performance and moisture absorption performance of the nylon 6 material are improved.