167 results about "Ornithine" patented technology

The invention belongs to the technical field of gene engineering and enzyme engineering, and particularly relates to an ornithine decarboxylase mutant as well as a coding gene, an expression vector, a recombinant strain and application thereof. The ornithine decarboxylase mutant is obtained by mutating wild ornithine decarboxylase ODC10 of which the amino acid sequence is shown as SEQ ID NO.1, the catalytic activity of the obtained mutant is improved, and the biosynthesis efficiency of 1, 4-butanediamine is favorably improved. The recombinant strain for expressing the ornithine decarboxylase mutant constructed by the invention can efficiently synthesize 1, 4-butanediamine, and the molar conversion rate of ornithine is remarkably improved, so that the production benefit is improved, and the production cost is reduced.

The invention discloses a genetically engineered bacterium for producing uridine diphosphate glucose as well as a construction method and application of the genetically engineered bacterium, and belongs to the technical field of genetic engineering. The following genes are overexpressed in the genetically engineered bacterium: a pyrimidine nucleoside operon gene, a carbamyl phosphate synthetase gene, an aspartate carbamyl transaminase gene, an orotic acid phosphoribose transferase gene, a phosphoribose pyrophosphate synthase gene, a uridylate kinase gene and a glucophosphate mutase gene. A uridine triphosphate glucose-1-phosphate uridine group transferase gene; the genetically engineered bacterium does not express the following genes: a purine nucleotide repressor protein gene, a deoxyribonucleoside triphosphate reductase gene, a nucleoside triphosphate pyrophosphohydrolase gene, a cytidine triphosphate synthase gene, a pyrimidine nucleotidase gene, a nucleotidase gene, an ornithine carbamoyltransferase gene, a UDPG dehydrogenase gene and a lacI repressor protein gene. The genetically engineered bacterium can be used for efficiently synthesizing UDPG by taking glucose as a substrate.

The invention relates to the field of fermentation processes, in particular to a method for preparing 1, 4-butanediol through multi-strain synergistic fermentation, which comprises the following steps: inoculating a first strain and a second strain into a culture medium containing glucose and / or xylose for synergistic fermentation, producing ornithine by the first strain by taking glucose and / or xylose as a substrate, and converting ornithine into 1, 4-butanediol by the second strain. The invention discloses 1, 4-butanediol. Glucose is converted into ornithine through the first strain, ornithine is converted into 1, 4-butanediol through the second strain, the first strain and the second strain are subjected to synergistic fermentation, preparation of 1, 4-butanediol through microbial synergistic fermentation is achieved, and higher metabolic capacity and higher production efficiency are achieved. The mixed sugar of xylose and glucose prepared from biomass raw materials is used as a substrate for fermentation, so that the method has the advantages that the raw materials are easy to obtain, renewable and low in cost, and the production cost can be reduced.

The specification describes an improved serum-free animal cell culture medium, which can used for the production of a protein of interest. Ornithine, or a combination of ornithine and putrescine can be added to serum-free media or chemically defined media to improve viable cell density, to reduce cell doubling time, and to increase the production of a protein of interest.

The invention discloses a rheumatoid arthritis diagnosis product and system and application thereof, and relates to the technical field of rheumatoid arthritis. The diagnostic marker comprises calcium, a ratio of arginine to ornithine, a ratio of phenylalanine to tyrosine, an anti-cyclic citrullinated peptide antibody and lysine. The invention provides a biomarker capable of accurately diagnosing rheumatoid arthritis RA, which comprises five indexes, namely calcium, the proportion of arginine to ornithine, the proportion of phenylalanine to tyrosine, an anti-cyclic citrullinated peptide antibody (anti-CCP) and lysine. The five indexes have the technical advantages of few indexes and excellent diagnosis performance. A diagnosis model constructed based on the five indexes has good sensitivity, specificity and accuracy. The kit can be used for diagnosis of rheumatoid arthritis.

The invention belongs to the technical field of microbial engineering and genetic engineering, and discloses escherichia coli for producing histidine as well as a construction method and application thereof. The escherichia coli contains a growth dependent promoter PprpB of a methyl isocitrate lyase gene prpB and / or a stable phase dependent promoter PydgI of an ornithine reverse transport protein / lysine permease gene ydgI. Escherichia coli for producing histidine uses growth dependent promoter PprpB of methylisocitrate lyase gene prpB to regulate expression of glutamine phosphoribose pyrophosphate transamidase gene purF and uses ornithine reverse transport protein / lysine permease gene (ydgI) The stable-phase dependent promoter PydgI regulates the expression of the ATP phosphoribosyltransferase gene hisG, so that the energy supply of thalli in early-stage cell growth and the metabolic flux of L-histidine in later-stage thalli production can be realized, and the balanced distribution of the metabolic flux between the cell growth of a microbial cell factory and the production of L-histidine is effectively balanced.

The invention provides a key enzyme for synthesizing butanediamine and application of the key enzyme, and belongs to the technical field of bioengineering. The invention provides a gene for synthesizing ornithine decarboxylase. The nucleotide sequence of the ornithine decarboxylase is shown as SEQ ID NO. 7. By further optimizing the reaction system, the butanediamine synthesis capability is improved, and butanediamine can be synthesized more efficiently.

The invention belongs to the technical field of microorganisms, and particularly relates to lactobacillus reuteri HYZQF isth-1 and application of the lactobacillus reuteri HYZQF isth-1 in preparation of hangover alleviating and liver protecting products, the lactobacillus reuteri HYZQF isth-1 provided by the invention is preserved in Guangdong Microbial Culture Collection Center on July 8, 2024, the preservation date is July 8, 2024, and the preservation number is GDMCC NO: 64833. The strain has the effects of dispelling the effects of alcohol and protecting the liver while regulating intestinal flora balance and promoting digestion and absorption of a human body, and can effectively relieve alcohol absorption of the body, so that discomfort after drinking is relieved and improved. After 4,500 billion of strains and 300mg of metabolites are given every day, mucopolysaccharide, ornithine, lactein and lecithin with necessary endogenous quantity can be continuously provided in the intestinal reproduction and growth process, so that the effects of dispelling the effects of alcohol and protecting the liver can be achieved, and the body health can be kept.

The invention provides an organoid basic culture medium and a method for maintaining and promoting organoid growth, and the organoid basic culture medium comprises a basic culture medium and an additive, the additives comprise taurine sodium 3-hydroxybutyrate, taurine, sodium hypoxanthate, creatinine, L-ornithine, urea, L-sodium lactate, putrescine dihydrochloride, anhydrous citric acid, ethanolamine, N-acetylglycine, alpha ketoglutaric acid, formic acid, malic acid, malonic acid, succinic acid, uric acid, ammonium metavanadate, manganese dichloride, sodium selenite, creatine, glycerol and uridine. Anhydrous sodium acetate and para aminobenzoic acid. When the organ-like basic culture medium is used for treating the organ-like, the activity of the organ-like can be effectively maintained, and basic nutrition and metabolism requirements of the organ-like can be provided, so that the organ-like has the advantages of high activity, fast proliferation and long-term stable passage, and is suitable for various types of organs and wide in application.

The invention relates to the technical field of detection, in particular to a kit for liquid phase detection of amino acid, a pretreatment method and a liquid phase detection method. The kit comprises a protective agent, an activating solution, a leacheate, an eluent and a resolvent, the protective agent comprises a first ammonium acetate solution; the activating solution comprises methanol and a second ammonium acetate solution; the leacheate comprises a formic acid aqueous solution and methanol; the eluent comprises a methanol solution with the pH value of 9 to 13. The detection method using the kit can meet the requirements of related laws and regulations on linearity, repeatability, batch-to-batch difference, accuracy and the like of cystine, lysine, ornithine and arginine, and has important reference significance for evaluating whether cystinuria exists or not.

The invention provides a metabonomics-based preeclampsia risk assessment prediction model construction method, a prediction model, application of metabolites, a metabolite detection kit, a prediction product, a prediction system and related application thereof. Specifically, clinical indexes and metabolite data of a pregnant woman serve as input, whether the pregnant woman suffers from preeclampsia or not serves as output, model construction is conducted through a machine learning method, and a preeclampsia risk assessment and prediction model is obtained. The metabolite is selected from at least one of 4-hydroxyphenyllactic acid, homovanillic acid, ornithine, sarcosine, adenosine monophosphate and 3-methylbutyryl carnitine. According to the invention, not only are six metabolite markers strongly associated with preeclampsia found, but also effective prediction of early preeclampsia and late preeclampsia is realized by establishing a prediction model, and the method has extremely high clinical value.

The invention belongs to the technical field of crop cultivation, and particularly discloses a two-component flavor enhancer for rice flavor enhancement as well as a preparation method and application of the two-component flavor enhancer for rice flavor enhancement, and the two-component flavor enhancer is prepared from a component A and a component B in a weight ratio of 1: (0.9-1.1), wherein the component A is prepared from the following raw materials in parts by weight: 80 to 120 parts of proline and 180 to 220 parts of ornithine; the component B is prepared from 2 to 5 parts of colloidal chitosan nano-selenium and 350 to 450 parts of zinc oxide quantum dots. The flavor enhancer developed in the invention is helpful for efficiently increasing the content of free proline and proline dehydrogenase, increasing the accumulation of 2-AP in rice grains, and effectively improving the rice fragrance.

The present invention provides an objective non-invasive method for examining renal dysfunction in cats. This method for testing renal dysfunction is a method for testing renal dysfunction in cats, and comprises a step for measuring the level of one or more D-amino acids selected from the group consisting of D-tyrosine, D-methionine, D-tryptophan, D-ornithine, and D-histidine in urine of a subject.

The invention belongs to the technical field of biological detection, and particularly relates to a biomarker composition and application thereof in methylation detection of an MGMT promoter. The biomarker composition is prepared from phospholipid (L5PL), creatinine (Creatinine) and ornithine (Ornithine) in the LDL-5 sub-component, and the biomarker composition is prepared from the phospholipid (L5PL), the creatinine (Creatinine) and the ornithine (Ornithine). Whether a glioblastoma patient carries MGMT promoter methylation or not is judged through a plurality of biomarkers, the method has the advantages of being good in sensitivity and specificity, small in patient trauma, capable of overcoming individual differences and the like, and a new solution is provided for prognosis evaluation of glioblastoma.

The present invention discloses Anti-hirsutism compositions, use and methods for managing hirsutism in a subject using the composition comprising turmeric oil standardized to contain Aromatic Turmerone (ar-Turmerone), Eflornithine; Nigella sativa extract; Oroxylum indicum extract; and Licorice extract either individually or combinations thereof. The invention also discloses use of the above composition in inhibiting the activity of ornithine decarboxylase and 5-α-reductase.

The invention discloses a polyarginine nano-particle as well as a preparation method and application thereof. The preparation method comprises the following steps: polymerizing ornithine-carboxylic anhydride (NCA) serving as a monomer with leucine to obtain a diblock polymer, or polymerizing the ornithine-carboxylic anhydride monomer, and then performing guanidination treatment and self-assembly to obtain the polyarginine nanoparticles. The polyarginine nano-particles prepared by the invention can be combined with free nucleic acid released by injured tissues or cells in traumatic brain injury (TBI) to remove the free nucleic acid and prevent downstream immune response reaction, so that the proinflammatory factor level is remarkably reduced, and the purposes of relieving inflammation and treating TBI are achieved; compared with the traditional cationic amino acid (polylysine), the polyarginine material adopted by the invention can effectively improve the prognosis of TBI patients. Therefore, the invention provides the nano material with remarkably improved DNA binding capacity and treatment effect, and the nano material has important significance in treatment of traumatic brain injury.

The invention relates to a method for determining enzyme activity of ODC1 (ornithine decarboxylase 1), which comprises the following steps: digesting, centrifuging and resuspending cells, and taking a part of sample to detect protein concentration; the method comprises the following steps: cracking cells by using a specially-made enzyme activity cell lysis solution and a protein lysis solution, and ensuring full cracking through ultrasonication; carrying out quantitative analysis on the protein in the lysate by adopting a BCA kit; in the enzyme activity detection stage, a cell lysis solution and an enzyme activity detection working solution containing a specific fluorescent probe (CB6) are mixed and react at 37 DEG C for a certain time, and then the change of fluorescence intensity is determined by a multifunctional fluorescence microplate reader, so that the enzyme activity of the ODC1 is evaluated. According to the method, accurate determination of the ODC1 enzyme activity is realized by optimizing cell treatment, protein quantification and enzyme activity detection processes. The method has the advantages of simplicity and convenience in operation, high sensitivity, good specificity and the like, and is suitable for the fields of basic research, drug screening and the like related to the ODC1 enzyme activity.

The present application relates to a recombinant microorganism of the genus Corynebacterium having an L-ornithine producing ability, comprising an amino acid transporter of the LysE / ArgO family derived from a Shewanella strain or a polynucleotide encoding an amino acid transporter of the LysE / ArgO family.

The present invention relates to a microorganism that produces 1,4-butanediol and a method for producing 1,4-butanediol using the same. Unlike naturally occurring microorganisms, the microorganism according to the present invention can efficiently produce 1,4-butanediol using ornithine as a carbon source.

A composition for relieving hangovers and protecting the liver, made from the following raw materials in the indicated weight ratios: 3-20 parts of kudzu root extract, 2-20 parts of Japanese raisin tree fruit extract, 1-12 parts of N-acetylcysteine, 1-12 parts of L-ornithine, 1-15 parts of L-glutamine, 0.2-8 parts of glycyrrhetinic acid glycyrrhiza extract, 0.05-5 parts of curcumin, 0.005-0.5 parts of piperine, 0.5-10 parts of vitamin C, 0.01-2 parts of B vitamins complex, 0.1-8 parts of electrolyte salts, and 1-25 parts of honey or oligosaccharides.

The invention discloses a reagent for enhancing aroma and improving quality of cigar tobacco leaves and application of the reagent, and relates to the technical field of tobacco fertilization and tobacco processing.The reagent comprises an ornithine solution with the mass concentration being 0.1-0.3%, or a glutamic acid solution with the mass concentration being 0.1-0.3%, or a proline solution with the mass concentration being 0.1-0.3%. The cigar tobacco aroma improving method has important significance on improving the quality of cigar tobacco raw materials, and is easy to operate, low in cost, economical and practical.

The invention relates to ornithine, or a pharmaceutically acceptable salt thereof, and at least one of phenylacetate and phenylbutyrate, to antagonists of TLR4, and to a combination of ornithine, or a pharmaceutically acceptable salt thereof, at least one of phenylacetate and phenylbutyrate, or a pharmaceutically acceptable salt thereof, and an antagonist of TLR4, and to medical uses of said agents and combination.

The invention provides a flora and an application of the flora in biodegradation of polyurethane plastics. The flora is prepared from raoultella ornithinolytica, klebsiella pneumoniae, coxakella rhizogenes and achromobacter xylosoxidans. According to the method, the polyurethane degrading flora is screened from the collected soil sample, the flora can efficiently degrade waterborne polyurethane and thermoplastic polyurethane elastomers with large chemical structure difference at the same time to degrade polyurethane plastic, the process is greatly simplified, the operation cost is reduced, traditional landfill incineration is replaced, green circulation of polyurethane is assisted, and the method has the advantages of being environmentally friendly and environmentally friendly. The method has important practical significance for solving the problem of environmental pollution caused by polyurethane waste and promoting green cyclic utilization of polyurethane materials.

Provided are methods for treating patients having ovarian cancer. The methods comprise administering a combined effective amount of SBP-101 and either doxorubicin or eflornithine.

Provided herein are fixed-dose combination formulations of a pharmaceutically effective amount of eflorithine together with a pharmaceutically effective amount of sulindac. Also provided are methods of use and of methods of manufacture of these formulations.

The invention discloses a metabolic biomarker for predicting the progress of hepatic fibrosis and application of the metabolic biomarker, a group of metabolic biomarkers with diagnostic value is screened out through a metabonomics method, and the metabolic biomarkers comprise acetic acid, alanine, arginine, citric acid, glucose, glycerol, lysine, ornithine, pyruvic acid, succinic acid and tyrosine. By using these biomarkers in combination, the accuracy and specificity of diagnosis can be further improved. A biological sample is collected from a tested object, the content of the biomarker is detected, and the content is compared with a preset threshold concentration for analysis, the detection means is nuclear magnetic resonance spectroscopy, and compared with the prior art, the sample pretreatment steps are simpler, the experiment operation is quicker, and the detection accuracy is higher. The problems that early hepatic fibrosis is not easy to perceive and a traditional diagnosis and treatment method is high in cost and long in consumed time are solved, and the accuracy of early hepatic fibrosis diagnosis is effectively improved.

The invention discloses application of a raoultella ornithine product in degradation of animal waste, and belongs to the technical field of animal waste treatment. The raoultella ornithinolyticus product comprises a raoultella ornithinolyticus solution with the concentration of 2 * 10 < 8 > cfu / mL to 2 * 10 < 9 > cfu / mL. Experiments prove that the raoultella ornithinolyticus solution with the specific concentration can promote the decomposition degree of animal waste, accelerate the composting fermentation speed, shorten the composting fermentation time and reduce the water content of fermented materials, so that the raoultella ornithinolyticus product has the advantages of high yield, high yield and the like. The degradation of organic matters in the animal waste is effectively promoted, the content of nutritional ingredients such as nitrogen, phosphorus and potassium is increased, and the method has important practical significance on resource utilization of agricultural wastes.

The invention relates to the technical field of plant genetic engineering, and discloses an overexpressed ornithine decarboxylase coding gene, an overexpressed transcription factor coding gene, application of biological materials of the overexpressed ornithine decarboxylase coding gene and the overexpressed transcription factor coding gene, and a method for increasing the nicotine content in tobacco. Meanwhile, the nicotine content in tobacco overexpressed with the two genes is remarkably increased compared with that of a wild type, and is far greater than the increasing amplitude of any one of the genes overexpressed independently, so that the problems that in the prior art, the nicotine content cannot be remarkably increased through gene coordinated regulation and control, and the nicotine increasing amplitude bottleneck is difficult to break through are effectively solved; powerful support is provided for high-nicotine tobacco cultivation.

An ornithine decarboxylase gene related to Auricularia auricula-judae degradation and application thereof belong to the field of bioengineering technology. In order to solve the technical problem that there is lack of key functional genes and molecular targets which can be used for early identification, anti-degradation molecular breeding and strain rejuvenation due to continuous subculture leading to degradation in Auricularia auricula-judae industry, based on the G1, G10 and G20 mycelium transcriptome difference analysis of Auricularia auricula-judae strain Heiwei S1908, the ornithine decarboxylase gene g4162 significantly differentially expressed between normal mycelium and G20 degraded mycelium is screened, the molecular function of the gene in the degradation of Auricularia auricula-judae subculture is determined, and the system biology information prediction of the gene coding protein is completed, which provides core gene resources and theoretical basis for the degradation mechanism research, molecular detection kit development, anti-degradation germplasm screening and gene breeding of Auricularia auricula-judae.

Disclosed in the present invention are an atosiban derivative or a pharmaceutically acceptable salt thereof, and the preparation and the use thereof. The structure of the atosiban derivative or the pharmaceutically acceptable salt thereof is as shown in general formula (I) or (II). On the basis of the present invention, fatty acid acylation is performed on an ornithine residue in an atosiban peptide sequence to prepare the derivative, which can effectively improve the antagonistic effect of a parent nucleus. The oxytocin antagonistic effect of the atosiban derivative prepared in the present invention is obviously higher than that of atosiban acetate, and the effect can be effectively used for preventing and / or treating premature delivery when used as an oxytocin antagonist to prepare a drug.