3858 results about "Ginseng" patented technology

The invention discloses an extraction and purification method and application of plant-derived exosome-like nanoparticles, and relates to the technical field of plant exosome preparation. The extraction and purification method comprises the following steps: washing fresh ginseng, airing for later use, adding a proper amount of buffer solution, mixing, breaking walls, filtering, centrifuging filtrate to obtain supernate, carrying out ultracentrifugation on the supernate to obtain ginseng exosome nano-particle precipitates, resuspending the precipitates, respectively adding pure water sucrose solutions with different concentrations, and carrying out ultracentrifugation to obtain ginseng exosome nano-particles; and re-suspending the target strip, and carrying out ultracentrifugation to remove redundant cane sugar so as to obtain the purified ginseng exosome-like nano-particles. The ginseng exosome-like nano-particles disclosed by the invention have a remarkable effect when being applied to cosmetics or medicines with skin photoaging resistance, skin oxidation resistance and wrinkle resistance. In an anti-UVB (Ultraviolet B) induced cell injury experiment, the ginseng exosome-like nano-particles have an excellent anti-UVB induced cell injury effect.

The invention discloses a ginseng seed lossless classification method and a ginseng seed lossless classification system, relates to the technical field of computer image detection, and solves the problem that in the prior art, a ginseng seed classification method based on image and spectral characteristics of ginseng seeds is lacked. Respectively collecting image data of the ginseng seeds and hyperspectral data of the ginseng seeds; respectively preprocessing the image data of the ginseng seeds and the hyperspectral data of the ginseng seeds; respectively carrying out feature screening on the preprocessed image data of the ginseng seeds and the preprocessed hyperspectral data of the ginseng seeds; fusing the image data of the ginseng seeds after feature screening and the hyperspectral data of the ginseng seeds; after the RBMO algorithm is improved, the RBMO algorithm is combined with the RF model to construct an ORBMO-RF model; and respectively inputting the fused image data of the ginseng seeds and the fused hyperspectral data of the ginseng seeds into an ORBMO-RF model for processing, thereby completing classification of the ginseng seeds.

The invention discloses a ginseng exosome, an extraction method thereof and application of the ginseng exosome in preparation of anti-photoaging skin care products, and belongs to the technical field of biological medicines. In order to solve the technical blank of ginseng exosomes in the aspect of light aging resistance in the prior art and solve the technical problems of complicated steps, long time consumption and small treatment capacity of exosome extraction by an ultracentrifugation method in the prior art, the invention provides an extraction method of ginseng exosomes. Therefore, the extraction rate of the ginseng exosome is improved, the treatment steps are simplified, the time is saved, and the purpose of producing the ginseng exosome in a large scale is achieved. The ginseng exosome obtained through the extraction method can be applied to preparation of anti-photoaging skin care products, and clinical application of the exosome is achieved.

The invention relates to the technical field of medicines, in particular to a medicinal and edible composition, a preparation method and application of the medicinal and edible composition in relieving acute alcoholic liver injury, and provides the medicinal and edible composition which is composed of ginseng, poria cocos, radix puerariae, fructus lycii, fructus gardeniae, fructus mume and oligo-walnut peptide. The medicinal and edible composition disclosed by the invention is non-toxic, harmless and high in nutritional value, and does not cause severe stimulation and harm to an organism. The health-care wine has a good effect of relieving liver injury, and is especially suitable for people with weakness due to long-term excessive drinking. Meanwhile, animal experiments and clinical verifications prove that the medicinal and edible composition for relieving acute alcoholic liver injury provided by the invention can effectively relieve liver functions, is one of effective methods for relieving symptoms after drinking, relieving inflammation, protecting the liver and improving the life quality, and has a wide application prospect. And a medicinal and edible combination product with good safety and good liver protection effect can be provided clinically. The important social significance is realized on relieving liver inflammation and preventing and treating occurrence and development of liver diseases.

The invention relates to the technical field of traditional Chinese medicine, in particular to a Mongolian medicine tea drink for relieving respiratory system abnormality and a preparation method and application thereof.The Mongolian medicine tea drink is prepared from rhododendron micranthum, pomegranate, amomum cardamomum, long pepper, cinnamon, safflower, agilawood, clove, myristica fragrans, fructus choerospondiatis, bistort rhizome, liquorice, tabasheer, radix aucklandiae, white raisin and crabs. The processed products of the Mongolian medicine components are weighed according to the formula dosage, then ground into coarse powder and bagged to prepare tea bags. The Mongolian medicine tea drink provided by the invention has the effects of moving Heli, relieving cough, reducing phlegm and diminishing swelling; the traditional Chinese medicine composition can be used for treating the symptoms such as' Bada dry Hiri 'dizziness, asthma, chronic bronchitis, indigestion disease, edema, edema and water tympanites.

The invention discloses a preparation method of an anti-aging cosmetic containing exosome, and relates to the technical field of cosmetic preparation, the cosmetic is prepared from the following components in percentage by mass: 1.01%-5.05% of exosome wrapped by liposome, 0.5%-3% of an antioxidant, 1%-3% of a centella asiatica extract, 1%-3% of a rhodiola rosea extract, 10%-15% of a humectant and the balance of a basic matrix; wherein the exosome wrapped by the liposome is a mixture formed by mixing an exosome secreted by adipose-derived mesenchymal stem cells and an exosome secreted by plant cells according to a mass ratio of 1.5: 1.2; the plant is ginseng. According to the skin care product, the antioxidant, the centella asiatica extract and the exosome act together to improve the DPPH clearance rate of the skin care product, the skin brightness is improved, the roughness is reduced, and the excellent anti-aging performance is achieved. The lipid exosome-antioxidant-centella asiatica ternary system disclosed by the invention has a multi-target synergistic effect.

The invention provides an anti-hair loss traditional Chinese medicine composition for scalp care as well as a preparation method and application thereof, and belongs to the technical field of shampoo. The traditional Chinese medicine composition is prepared from the following raw materials in parts by mass: 8 to 15 parts of ginseng, 20 to 30 parts of cacumen biotae, 12 to 20 parts of radix polygoni multiflori preparata, 15 to 25 parts of radix salviae miltiorrhizae, 18 to 28 parts of herba ecliptae and 10 to 16 parts of rhizoma chuanxiong. The traditional Chinese medicine extract prepared from the raw materials is used as an effective component of the shampoo, and ginseng can tonify qi, strengthen hair and enhance hair follicle activity; the cacumen biotae prevents hair loss and promotes hair growth; prepared fleece-flower root can blacken hair and nourish; salvia miltiorrhiza controls oil, inhibits bacteria and relieves alopecia seborrhoeica; herba ecliptae cools blood and nourishes hair; ligusticum wallichii can promote blood circulation to remove blood stasis and promote scalp microcirculation; various traditional Chinese medicine extracts jointly exert the effects of preventing hair loss, promoting hair growth, controlling oil, removing dandruff, nourishing and repairing.

The invention provides a combined fermented product of ginseng, astragalus membranaceus and donkey-hide gelatin as well as a preparation method and application of the combined fermented product, and relates to the technical field of pharmaceutical preparations. The combined leavening is obtained by fermenting ginseng, astragalus membranaceus and donkey-hide gelatin, and the mass ratio of the ginseng to the astragalus membranaceus to the donkey-hide gelatin is (1-3): (10-30): 1. The composition of ginseng, astragalus membranaceus and colla corii asini is subjected to probiotic fermentation, macromolecular saponin is converted into aglycone or secondary saponin with smaller molecules, protein is split into peptide chains with smaller molecules, polysaccharide is split into sugar chains with smaller molecules, absorption and utilization of effective components of the medicine are facilitated, and the effect of tonifying qi and blood can be rapidly achieved.

The invention discloses application of a compound shown as a formula I or salt or deuterated substance thereof in preparation of an anti-saccharification agent, in which ring A is a benzene ring or a five-membered ring containing O or S; the ring A may be substituted by m Ra, m being 0, 1, 2, 3 or 4; ra is hydroxyl, methyl, methoxyl, nitro or dimethylamino, and the deuterated substance is a partial deuterated substance and / or a perdeuterated substance. In the experiment of inhibiting generation of AGEs, compared with an Amino guanidine AG (Amino guanidine, AG), the anti-saccharification effect is obvious: under the low concentration, the anti-saccharification inhibition rate (the final administration concentration is 33 mu M) of the aromatic methylene acetone active small molecule compound protected by the invention is between 10% and 58%, and is far higher than the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG under the same final administration concentration (33 mu M), and the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG is far higher than the anti-saccharification inhibition rate (8.4%) of the Amino guanidine AG under the same final administration concentration (33 mu M) of the Amino guanidine AG. And the anti-saccharification inhibition rate of the radix codonopsis AG approaches to the final administration concentration (1mM) and is 57%. # imgabs0 #

The invention discloses a composition with homology of medicine and food and application thereof in relieving physical fatigue, the composition is selected from at least two of ginseng, maca and green tea, and the obtained composition can synergistically regulate oxidation resistance, calcium ion metabolism and energy metabolism, synergistically improve the ATP content and glycogen level of an organism, accelerate removal of lactic acid and urea nitrogen and improve the physical fatigue. And the synergistic effect of the ginseng, the maca and the green tea is better than that of single use of the ginseng, the maca and the green tea. The composition provided by the invention is verified by a standard method of a health-care food function test and evaluation method (2023 edition), and is proved to have a remarkable effect of relieving physical fatigue.

The invention relates to a preservation method and application of panax notoginseng germplasm resources, and relates to the technical field of panax notoginseng germplasm preservation. The preservation method comprises the following steps: (1) suspending pseudo-ginseng embryogenic calluses and a WPM culture medium, and screening and collecting to obtain pseudo-ginseng embryogenic cell clusters with the size of 0.45-1.5 mm; (2) transferring the panax notoginseng embryogenic cell cluster into a cryopreservation tube, and adding a loading solution for loading treatment to obtain a loaded embryogenic cell cluster; (3) carrying out vitrification treatment on the loaded panax notoginseng embryogenic cell cluster and a vitrification solution in a cryopreservation tube to obtain a vitrified panax notoginseng embryogenic cell cluster; (4) placing the cryopreservation tube filled with the vitrified pseudo-ginseng embryogenic cell cluster in liquid nitrogen for preservation; and after preservation, carrying out recovery culture on the pseudo-ginseng embryogenic cell cluster. According to the method, the survival rate reaches 90% after preservation, pseudo-ginseng plants can be rapidly differentiated, the differentiated pseudo-ginseng plants survive after being transplanted under natural conditions, the transplanting survival rate reaches 90%, and the pseudo-ginseng plants grow well in the later period.

The invention discloses a preparation method of a novel ginseng exosome nano drug delivery system, and mainly relates to the field of functional medical materials. Comprising the following steps: S1, preparing a ginseng exosome crude extract; s2, centrifuging the crude extract and taking supernate; s3, centrifuging the supernate, and taking the precipitate to obtain an exosome resuspension; s4, preparing a purified ginseng exosome; s5, preparing kaempferol nano-enzyme freeze-dried powder; s6, mixing the ginseng exosome solution with the kaempferol nano-enzyme freeze-dried powder to obtain a ginseng exosome kaempferol nano-enzyme mixed solution; and S7, separating the ginseng exosome loaded with the kaempferol nano enzyme to obtain the ginseng exosome nano delivery system. The preparation method has the beneficial effects that the plant active ingredient ginseng exosome is taken as a delivery carrier to carry the water-soluble kaempferol nano-enzyme, and the water-soluble kaempferol nano-enzyme and the ginseng exosome play a synergistic role, so that the effective loading capacity of the exosome on the medicine is improved, the concentration of the medicine is improved, and the preparation method plays a role in various diseases such as wounds and inflammation; the traditional Chinese medicine composition can be better applied to treatment of acute lung injury.

The invention belongs to the technical field of biological bacterium agents, and particularly relates to a penicillium photosporum LZ-3, a bacterium agent and application of the penicillium photosporum LZ-3 in biological prevention and control and growth promotion of panax notoginseng root rot. The preservation number of the penicillium glabrata LZ-3 disclosed by the invention is CCTCC (China Center for Type Culture Collection) NO: M2024280. On the basis, the invention further provides a microbial inoculum containing the penicillium photosporum LZ-3, and the penicillium photosporum LZ-3 and the microbial inoculum containing the penicillium photosporum LZ-3 can effectively relieve continuous cropping obstacles of the panax notoginseng and antagonize fusarium fungi and sphaerotheca fungi so as to achieve the effect of preventing and controlling the root rot of the panax notoginseng. Meanwhile, the growth of the pseudo-ginseng can be promoted and the saponin content in the pseudo-ginseng can be increased.

The invention belongs to the technical field of microorganisms, and particularly relates to pseudomonas monteilii BDLZ-3 and application thereof in relieving continuous cropping obstacles of crops. The invention discloses a strain of pseudomonas monteilii BDLZ-3, the preservation number of which is CGMCC (China General Microbiological Culture Collection Center) No.34349. The pseudomonas monteilii BDLZ-3 is separated and screened from the nature for the first time, phenolic acid autotoxic substances generated in the ginseng continuous cropping obstacle process can be efficiently degraded, the degradation rate of benzoic acid can reach 95.36%, various phenolic acids such as vanillic acid, cinnamic acid, palmitic acid and gallic acid can be effectively degraded, and the degradation spectrum is wide; when the pseudomonas monteilii BDLZ-3 is applied in a field, the content of benzoic acid in soil can be effectively reduced, meanwhile, the occurrence of ginseng root diseases can be controlled to a certain extent, the prevention and treatment effect reaches 41.23%, the growth indexes such as root length and root thickness are remarkably improved, and the yield increasing effect is achieved.

The invention provides application of camphene in inducing pseudo-ginseng resistance to relieve leaf black spot, and relates to the technical field of pseudo-ginseng planting. According to the method, camphene is placed on continuous cropping soil to fumigate pseudo-ginseng in advance to induce the resistance of the pseudo-ginseng to leaf black spot infection in the later stage, and test results show that the pseudo-ginseng after being fumigated and induced by the camphene is infected by black spot pathogenic bacteria, so that the resistance of the pseudo-ginseng can be remarkably enhanced, and the attack area of the black spot can be reduced.

The invention discloses a flexible clamping jaw capable of automatically adjusting clamping force based on deformation measurement, and belongs to the field of vegetable and fruit picking devices.The flexible clamping jaw comprises a rotating mechanism, three clamping jaw mechanisms are symmetrically installed at the front end of the rotating mechanism through an installation disc, and the rotating mechanism and the clamping jaw mechanisms form a picking mechanism; the rotating mechanism can drive the three clamping jaw mechanisms to rotate through an internal rotating motor, each clamping jaw mechanism comprises a flexible clamping jaw, the tail of each flexible clamping jaw is driven by a connected cam connecting rod mechanism to swing, and the back face of each clamping jaw mechanism is fixedly provided with a monitoring mechanism. The monitoring mechanism monitors the deformation quantity of the flexible clamping jaw through a camera. According to the flexible clamping jaw, the clamping force is accurately controlled through a camera and an algorithm, and fruits are protected against damage; the three-jaw structure is stable, the process is automatic, and the efficiency is improved; ginseng can be flexibly set according to vegetables and fruits, and the adaptability is wide; data after picking is driven and optimized, long-acting operation of equipment is facilitated, and the cost is reduced.

The invention belongs to the technical field of microorganisms, and particularly relates to a P.adami FJ08 strain, a fungicide, a preparation method and application thereof, which can be used for inhibiting soil-borne diseases of radix pseudostellariae. The invention discloses a P.aryabhattai FJ08, the P.aryabhattai FJ08 is preserved in the Microbial Culture Collection Center of Guangdong Province, the preservation number is GDMCC No: 65907, and the preservation date is March 24, 2025. The P.aryabhattai FJ08 has the advantages that the P.aryabhattai FJ08 can be used for preparing the P.aryabhattai FJ08; the P.aryabhattai FJ08 is separated from the radix pseudostellariae seed ginseng, is safe and reliable, and has the capabilities of inhibiting the growth of fusarium oxysporum, producing IAA, producing ammonia, dissolving potassium and dissolving phosphorus, and the growth of pathogenic fusarium oxysporum can be effectively inhibited by soaking the radix pseudostellariae seed ginseng with the P.aryabhattai FJ08.

The invention discloses bird's nest composite honey suitable for women. The bird's nest composite honey is prepared from the following raw materials in parts by weight: 1-10 parts of bird's nests, 1-10 parts of ginseng, 1-10 parts of donkey-hide gelatin, 1-10 parts of lucid ganoderma, 1-10 parts of walnuts, 5-50 parts of rhizoma polygonati, 5-50 parts of fingered citron, 5-50 parts of dried orange peel, 5-50 parts of dried ginger, 5-50 parts of raw malt and 700-970 parts of refined brown sugar. The cubilose composite honey suitable for women is prepared by taking refined brown sugar as a main raw material and combining more than ten precious traditional Chinese medicinal materials such as cubilose, ginseng, donkey-hide gelatin, lucid ganoderma, walnuts, rhizoma polygonati, fingered citron, dried tangerine or orange peel, dried ginger and raw malt, has the characteristics of being good in taste, quick to dissolve, easy to absorb, pure in color and luster, sweet and lingering, simple and convenient to brew one cup of one piece of sugar; the traditional Chinese medicine composition is suitable for women with yellow, dark and blood-free faces and less menstruation, can maintain beauty, keep young and improve menstruation, and has an obvious menstruation continuing effect on menopausal groups.

The invention discloses a traditional Chinese medicine composition for treating constipation and a preparation method thereof, the traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20g of radix aconiti lateralis preparata, 5-15g of fructus evodiae, 20-30g of herba epimedii, 35-45g of radix astragali, 10-20g of ginseng, 5-15g of rhizoma atractylodis macrocephalae, 20-35g of semen coicis, 50-60g of fructus aurantii immaturus, 10-20g of betel nut and 5-15g of honey-fried licorice root. Comprising the following steps: (1) accurately weighing the raw materials in parts by weight, respectively pulverizing, sieving with a 200-mesh sieve, and mixing to obtain mixed medicinal powder for later use; and 2) adding the mixed medicine powder into a full-automatic traditional Chinese medicine watered pill machine, adding cooked millet accounting for 1-5% of the total amount of the mixed medicine powder, starting the full-automatic traditional Chinese medicine watered pill machine, and processing to obtain watered pills.

The invention relates to the field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for tonifying qi and blood and treating hypomenorrhea caused by deficiency of qi and blood and a preparation method thereof. The composition is prepared by carefully selecting a plurality of traditional Chinese medicinal materials with the effects of tonifying qi and blood, regulating menstruation and nourishing blood and combining the traditional Chinese medicinal materials according to a specific proportion, and comprises ginseng, codonopsis pilosula, lucid ganoderma, astragalus membranaceus, angelica sinensis, peach kernels, donkey-hide gelatin, longan aril, Chinese dates, Chinese yams, radix polygonati officinalis, fructus lycii, poria cocos, roses and pericarpium citri reticulatae. The traditional Chinese medicine composition is prepared through the processes of extraction, double-effect concentration, filtration, blending and the like through scientific proportioning and a unique processing technology, aims to effectively improve the symptom of scanty menstruation caused by deficiency of qi and blood of women, recover normal menstruation, condition the body and improve the overall health level, has good safety and effectiveness, and is worthy of popularization and application. And a new choice is provided for health management of women. The traditional Chinese medicine composition for tonifying qi and blood and treating hypomenorrhea caused by deficiency of qi and blood is safe, small in side effect, efficient, convenient and good in taste.

The invention discloses a large-scale preparation method of ginseng exosomes, which comprises the following steps: culturing calluses: inoculating explants into an induction culture medium until ginseng calluses are generated; proliferation of the calluses: inoculating the newborn calluses into a proliferation culture medium for proliferation culture; inducing germination and rooting: after the ginseng callus is amplified, transplanting the ginseng callus into a germination induction culture medium until the ginseng callus is germinated, and then transferring the ginseng callus into a rooting culture medium for culturing and rooting; and preparing the exosome, namely grinding root hairs grown from the ginseng callus regeneration plant, uniformly mixing the ground root hairs with PBS (Phosphate Buffer Solution) or performing liquid culture on ginseng suspension cells, and finally performing centrifugal treatment to obtain the ginseng exosome. According to the large-scale preparation method of the ginseng exosome, the endotoxin pollution in the growth and development process is reduced through ginseng callus culture rooting, and then the ginseng exosome is prepared, so that the large-scale preparation requirement in modern production and processing is met.

The invention discloses a preparation method of a doxorubicin-loaded ginseng selenium polysaccharide nano-carrier with immunoregulation and liver cancer cell inhibition functions, and aims to solve the problems of toxic injury of tissues and organs and inhibition of an immune system caused by an antitumor drug doxorubicin. According to the invention, deoxycholic acid is grafted on the ginseng selenium polysaccharide, so that the ginseng selenium polysaccharide is hydrophobized and forms an amphiphilic polysaccharide polymer, and the limitations of large molecular weight, low bioavailability and the like of the ginseng selenium polysaccharide are overcome. The self-assembled ginseng selenium polysaccharide nanoparticles are used as carriers for delivering antitumor drugs, so that the self-assembled ginseng selenium polysaccharide nanoparticles have an immunoregulation effect and can effectively improve side effects such as immunosuppression caused by the antitumor drugs; and the carrier also has a pH sensitive response release characteristic, so that the targeting effect of the medicine in a tumor area is enhanced, and the innovative carrier has a good clinical application prospect.

The embodiment of the invention provides a method for regulating and controlling the form of ginseng adventitious roots and saponin accumulation. The method comprises the following steps: (a) carrying out mutagenesis treatment on initial ginseng adventitious roots by using ethyl methane sulfonate (EMS); (b) performing multiple rounds of screening and subculture to obtain to-be-selected ginseng adventitious roots with different forms and saponin accumulation; and (c) determining one or more target genes for regulating and controlling the form of the ginseng adventitious root and saponin accumulation based on the ginseng adventitious root to be selected.

The invention discloses a medicinal and edible anti-alcoholism composition and a preparation method thereof, and relates to the crossing field of biotechnology and food and medicine, the composition comprises the following components: dried orange peel powder, vitamin C, glutathione, phellinus igniarius extract, polygonatum sibiricum extract, oriental wormwood extract, isatis root extract, schisandra chinensis extract, ginseng powder, lucid ganoderma extract and licorice extract. Silymarin, puerarin, white tomato powder, watermelon powder, fucoidin, tomato powder, mung bean powder, celery powder, turmeric powder, hawthorn fruit powder, honey powder, corn peptide, soybean peptide powder, xanthan gum and stevioside are added. The composition provided by the invention has the effect of increasing the content of ethanol dehydrogenase and acetaldehyde dehydrogenase in blood after drinking, and has the capability of promoting alcohol metabolism.

The invention discloses a plant beverage capable of dispelling effects of alcohol, protecting liver and resisting fatigue and a preparation method thereof. The health-care tea is characterized by being prepared from the following raw materials in parts by weight: 1-12 parts of ginseng, 1-24 parts of rhizoma polygonati, 1-40 parts of radix puerariae, 1-24 parts of ampelopsis grossedentata, 1-24 parts of semen cassiae, 1-24 parts of mulberries, 1-40 parts of liquorice, 1-20 parts of hippophae rhamnoides fruit powder, 1-24 parts of poria cocos, 1-24 parts of smoked plums, 1-24 parts of rhizoma phragmitis, 1-40 parts of hovenia dulcis thunb, 1-24 parts of hawthorns, 1-24 parts of pericarpium citri reticulatae, 1-24 parts of lucid ganoderma and a proper amount of water. The preparation method comprises the following steps: pretreating raw materials, soaking, decocting, filtering, concentrating, blending, filling, sterilizing, inspecting and packaging. The beverage has the beneficial effects that the beverage has remarkable effects of dispelling effects of alcohol, protecting liver and resisting fatigue; no side effect exists; the mouthfeel is good; the preparation method is simple and low in cost; the tea is suitable for different people.

The invention belongs to the technical field of functional foods and health foods, and particularly relates to an anti-saccharification and anti-aging aronia melanocarpa fruit composition and a preparation method thereof. The composition is prepared from the following raw materials in parts by weight: 150 to 400 parts of aronia melanocarpa fruit fermentation liquor, 10 to 80 parts of lycium ruthenicum, 10 to 80 parts of ginseng, 0.5 to 2.5 parts of grape seed extract, 10 to 70 parts of collagen peptide, 0.5 to 4 parts of sodium hyaluronate, 0.1 to 0.8 part of resveratrol and 0.03 to 0.3 part of yeast extract. The content of type I collagen peptide in the skin is increased, the generation of in-vitro anti-glycation end products is inhibited, and the anti-glycation and anti-aging effects are achieved.

The invention belongs to the technical field of biological medicine, and particularly relates to red ginseng fermentation broth and a preparation method and application thereof.The preparation method of the red ginseng fermentation broth comprises the following steps that red ginseng powder is dissolved in water, and a red ginseng fermentation medium is obtained by breaking cell walls in red ginseng; mixing the seed solution of the plant lactobacillus and the seed solution of the fermentation mucus lactobacillus to obtain a compound strain seed solution; inoculating the compound strain seed solution into a red ginseng fermentation culture medium for fermentation culture to obtain a fermentation solution; centrifuging the fermentation liquor, collecting supernate, extruding and filtering the supernate, and collecting filtrate, so as to obtain the red ginseng fermentation liquor. According to the invention, compound strain fermentation is adopted, and a mechanical extrusion mode is combined to increase the content of total saponins of ginseng. According to the method disclosed by the invention, the concentration of the total saponins of ginseng is increased from 2.19 mg / mL to 7.53 mg / mL.

The invention relates to the field of ginseng planting, in particular to bacillus subtilis and application thereof in aluminum stress resistance of ginseng. The invention relates to bacillus subtilis YX1, which is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus subtilis YX1 is CGMCC No.31921. The bacillus subtilis YX1 is separated from rhizosphere soil of the understory ginseng, shows relatively strong aluminum resistance, can generate exopolysaccharides, is effectively colonized in the root system of the ginseng, and has a positive effect on salt stress resistance of the ginseng, especially aluminum stress.

The invention relates to a preparation method of traditional Chinese medicine residue metabiotics and application of the traditional Chinese medicine residue metabiotics in fish feed, and belongs to the technical field of feed additives. Beneficial bacteria such as bacillus subtilis, lactobacillus lactis, bifidobacterium longum and saccharomyces cerevisiae are separated from intestinal contents of grass carp and are subjected to enlarged culture, then traditional Chinese medicine residues such as fresh ginger, folium mori, ginseng, fructus lycii, rhizoma curcumae longae, liquorice and mint are added, staged mixed fermentation culture is performed, obtained metabolites are added into fish feed, and the fish feed is obtained. The feeding rate and the growth rate of fishes such as mandarin fish and micropterus salmoides are effectively improved, and the resistance is enhanced.

A ginseng year identification method relates to the technical field of traditional Chinese medicinal material identification. The problems that an existing chemical or biological analysis method needs to damage a sample and is tedious, the nondestructive testing requirement cannot be met, and the accuracy rate cannot be guaranteed when a spectrum technology is adopted for detection and identification are solved. The method comprises the following steps: step 1, carrying out spectrum scanning on ginseng samples to obtain spectrum data of the ginseng samples in different years; 2, extracting the spectral data, and establishing a spectral data set; 3, constructing a local search heuristic cross model based on a genetic algorithm according to the spectral data set; and 4, performing ginseng year identification according to the local search heuristic cross model. According to the method, the limitation in the traditional method is overcome, higher identification precision and stronger analysis capability are brought, and the method is applied to the field of ginseng year identification.