Medicinal and edible composition and application thereof in relieving physical fatigue

Abstract

The invention discloses a composition with homology of medicine and food and application thereof in relieving physical fatigue, the composition is selected from at least two of ginseng, maca and green tea, and the obtained composition can synergistically regulate oxidation resistance, calcium ion metabolism and energy metabolism, synergistically improve the ATP content and glycogen level of an organism, accelerate removal of lactic acid and urea nitrogen and improve the physical fatigue. And the synergistic effect of the ginseng, the maca and the green tea is better than that of single use of the ginseng, the maca and the green tea. The composition provided by the invention is verified by a standard method of a health-care food function test and evaluation method (2023 edition), and is proved to have a remarkable effect of relieving physical fatigue.

Description

Technical Field

[0001] The present invention relates to the field of medicine and food technology, and in particular to a medicine-food homologous composition and application thereof in relieving physical fatigue. Background Art

[0002] Fatigue is a common physiological phenomenon in human life, and sports fatigue refers to the phenomenon that in the physiological process, one cannot continue to function at a specific level or cannot maintain a predetermined exercise intensity. Some food-derived active ingredients can significantly regulate the body's energy metabolism and metabolite accumulation and relieve physical fatigue through multiple pathways such as anti-oxidation, reducing metabolite accumulation, anti-inflammation, regulating energy metabolism and intestinal microecology.

[0003] At present, there are many kinds of health care products for relieving fatigue in China, but the existing products have the following limitations and shortcomings: the formula ingredients are complex, and the selection and proportion of raw materials lack scientific basis; the mechanism of action of compound formula products is unclear; most products are formulated based on actual application experience, lack scientific demonstration and experimental verification; the interaction relationship between the various ingredients in the formula to relieve fatigue is unclear. With the continuous improvement of consumers' health awareness, it is easier for people to recognize products with few side effects and health care functions developed from natural plants, especially traditional edible resources. Therefore, it is of great significance to develop a scientifically verified, safe, and directly edible product that effectively relieves physical fatigue, which is composed of traditional medicinal and edible products or active ingredients from natural plants.

[0004] In view of this, the present invention is proposed. Summary of the invention

[0005] The purpose of the present invention is to provide a medicine-food composition and its application in relieving physical fatigue. After experimental verification, it is proved that the composition can effectively relieve physical fatigue.

[0006] To achieve the above purpose, the technical solution adopted by the present invention to solve the technical problem is: The invention provides a composition with the effect of synergistically alleviating physical fatigue, wherein the components of the composition are at least two selected from ginseng, maca and green tea.

[0007] Ginseng is a plant of the Araliaceae family ( Panax ginseng The dried root and rhizome of Acacia camey. have the functions of greatly replenishing vital energy, restoring pulse and strengthening deficiency, nourishing spleen and lung, promoting body fluid and nourishing blood, calming the mind and improving intelligence. It is used for weakness and collapsing, cold limbs and weak pulse, poor appetite due to spleen deficiency, wheezing and coughing due to lung deficiency, thirst due to loss of body fluid, internal heat and thirst, deficiency of qi and blood, weakness due to long-term illness, palpitations and insomnia, impotence and cold uterus.

[0008] Inca radish (scientific name: Lepidium meyeniiWalp., also known as maca, is an edible resource. Its root can be used as medicine, which is the source of the medicinal material maca. Maca is rich in various alkaloids, taurine, protein, etc., and has the effects of enhancing physical strength, reducing stress, and enhancing sexual function.

[0009] Green tea is an unfermented tea. Due to its characteristics, it retains more natural substances in fresh leaves. Tea polyphenols and caffeine retain more than 85% of the fresh leaves, chlorophyll retains about 50%, and vitamin loss is also relatively small. Therefore, green tea contains powerful antioxidants and vitamin C, which can not only remove free radicals in the body, but also secrete hormones to fight stress. The small amount of caffeine contained in green tea can stimulate the central nervous system and boost spirits.

[0010] In the early stage of the experiment, the present invention screened and obtained a plurality of food-derived ingredients that can relieve physical fatigue. By analyzing and comparing the effects of each single ingredient on indicators such as the body's ATP content, glycogen level, and the ability to clear lactic acid and urea nitrogen, it was found that green tea can improve the body's antioxidant capacity, reduce lactic acid and urea nitrogen content, and relieve fatigue; maca can improve the body's glycogen level, regulate energy metabolism, and prolong the exercise time of mice; ginseng relieves fatigue by improving the energy supply efficiency of the body's skeletal muscle mitochondria and accelerating the free radical clearance. Therefore, ginseng, maca, and green tea are selected to be compounded in pairs or in three combinations. The compounded composition can play a synergistic role in terms of the body's ATP content, glycogen level, and the ability to clear lactic acid and urea nitrogen, so that the anti-fatigue effect after compounding is significantly improved.

[0011] Based on this, the composition scheme that can be provided by the present invention can be: a composition formed of two ingredients, namely ginseng and maca, ginseng and green tea, maca and green tea, or a composition formed of three ingredients, namely ginseng, maca and green tea.

[0012] In some embodiments, when preparing the above composition, ginseng, maca and green tea are all processed into powder state for compounding.

[0013] The present invention does not limit the sources, preparation or extraction methods of the above components, and they can be purchased from the market or prepared by conventional processing methods in the art.

[0014] In some embodiments, the composition is composed of the following components and their weight parts by weight: 1-7 parts of ginseng and 3-8 parts of maca. Specifically, the ratio of ginseng to maca can be: 1:3, 1:8, 2:3, 2:8, 3:3, 3:8, 4:3, 4:8, 5:3, 5:8, 6:8, 7:3 or 7:8, etc., or any other ratio between 1-7:3-8.

[0015] In some embodiments, the composition is composed of the following components and their weight parts by weight: 1-7 parts of ginseng and 2-10 parts of green tea. Specifically, the ratio of ginseng to green tea can be: 1:2, 1:10, 2:2, 2:10, 3:2, 3:10, 4:2, 4:10, 5:2, 6:2, 6:10, 7:2 or 7:10, etc., or any other ratio between 1-7:2-10.

[0016] In some embodiments, the composition is composed of the following components and their weight parts by weight: 3-8 parts of maca and 2-10 parts of green tea. Specifically, the ratio of maca to green tea can be: 3:2, 3:10, 4:2, 4:10, 5:2, 6:2, 6:10, 7:2, 7:10, 8:2 or 8:10, etc., or any other ratio between 3-8:2-10.

[0017] In some embodiments, the composition is composed of the following components and their weight parts by weight: 1-7 parts of ginseng, 3-8 parts of maca, and 2-10 parts of green tea. Specifically, the ratio of ginseng, maca and green tea can be: 1:3:2, 1:3:10, 1:8:2, 1:8:10, 7:3:2, 7:3:10, 7:8:2 or 7:8:10, etc., or any other ratio between 1-7:3-8: 2-10.

[0018] In some embodiments, the composition is composed of the following components and their weight parts by weight: 3 parts of ginseng, 7 parts of maca, and 6 parts of green tea. In some animal experiments, it can be found that the composition of the present invention has the best effect on the body's antioxidant, calcium ion metabolism, energy metabolism, and glycogen level regulation when the mass ratio is 3:7:6, and the combined index CI value is lower, which further indicates that under the above ratio conditions, the composition can more effectively relieve physical fatigue.

[0019] The combination index method is a method for evaluating the combined effects of drugs. It is particularly suitable for evaluating the interaction between two or more ingredients after compounding. This method is based on the dose-effect curve of the drug. By calculating the ratio of the actual effect to the theoretical effect during combined use, it is determined whether the combined use has a synergistic effect, an additive effect, or an antagonistic effect. A combination index (CI) less than 1 indicates a synergistic effect, 1 indicates an additive effect, and greater than 1 indicates an antagonistic effect. Therefore, through experiments to screen out food-derived ingredients that are better at relieving physical fatigue, and combining the combination index method to evaluate the interaction relationship between food functional ingredients, it is very beneficial to the development of synergistic functional food formulas.

[0020] The present invention verifies the effect of the above scheme by the combined index method, and the results show that no matter it is a composition formed by two components or a composition formed by three components, it can improve the swimming distance, recovery rate and ATP, reduce the lactic acid content, and increase the expression of Nrf2, atp2b1 and g6pca genes. Among them, the Nrf2 gene is an oxidative stress-related gene, the atp2b1 gene is a calcium ion metabolism-related gene, and the g6pca gene is an energy metabolism-related gene. By detecting the expression changes of these three genes, it can be proved that the various components in the composition of the present invention can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism pathways, thereby relieving physical fatigue.

[0021] At the same time, the three-component composition of the present invention has also been verified by the standard method of "Health Food Function Test and Evaluation Method (2023 Edition)". The results show that this composition can prolong the swimming time of mice, reduce lactic acid and urea nitrogen content, and increase muscle glycogen and liver glycogen content. Mammalian experiments further prove that the three-component composition of the present invention can significantly prolong the exhaustive swimming time by reducing the accumulation of metabolic products in the body and increasing the body's glycogen level, thereby relieving physical fatigue.

[0022] Based on the above, the present invention can also provide the use of the above composition in the preparation of a product for relieving physical fatigue; or, the present invention can also provide the use of the above composition in the preparation of a product for improving ATP energy metabolism or reducing lactic acid; or, the present invention can also provide the use of the above composition in the preparation of a product for regulating the expression of Nrf2, atp2b1 and g6pca genes.

[0023] The composition of the present invention has broad application prospects and can be widely used in the fields of food, health care products, medicines, etc. As people's demand for relieving physical fatigue continues to increase, the product is expected to achieve good economic and social benefits in the market. In this regard, the present invention can also provide a food or health care product, the active ingredient of which is the above composition.

[0024] In some embodiments, the composition of the present invention can be made into food using techniques known to those skilled in the art.

[0025] In some embodiments, the food or health product prepared from the composition of the present invention may further include food-acceptable auxiliary materials, such as solvents, adhesives, fillers, sugars, sweeteners, and the like.

[0026] In some embodiments, the food or health product prepared from the composition of the present invention can be of the following types: tablets, capsules, oral liquids, solid beverages, pills, granules, decoctions, pastes, lotions, pills, syrups or tea bags.

[0027] In some embodiments, the compositions of the present invention can be formulated into medicines suitable for topical administration using techniques known to those skilled in the art.

[0028] In some embodiments, the drug prepared by the composition of the present invention may also include pharmaceutically acceptable excipients, such as: buffers, emulsifiers, suspending agents, decomposers, disintegrants, dispersants, binders, excipients, stabilizers, chelating agents, diluents, gelling agents, preservatives, wetting agents, lubricants, absorption delaying agents, liposomes, etc.

[0029] In some embodiments, the drug prepared from the composition of the present invention can be in the following dosage forms: tablets, oral liquids, hard capsules, soft capsules, granules, powders or pills.

[0030] In the above-mentioned foods and medicines, the addition amount and addition ratio of the composition of the present invention and related auxiliary materials can be adjusted according to actual needs, and the present invention does not limit this.

[0031] The present invention has the following beneficial effects: (1) The active ingredients of the composition of the present invention are ginseng, maca and green tea. The composition can synergistically regulate antioxidant, calcium ion metabolism and energy metabolism, synergistically improve the body's ATP content and glycogen level, accelerate the clearance of lactic acid and urea nitrogen, prolong exercise time, and significantly relieve physical fatigue. The synergistic effect is stronger than that of using ginseng, maca and green tea alone.

[0032] (2) The composition of the present invention has simple ingredients and all come from natural plants. At the same time, through scientific demonstration and experimental verification, the mechanism of action of the composition is clear, and the interaction relationship between the various ingredients in relieving fatigue is clear, which has the advantages of being green and safe.

[0033] (3) The preparation process of the composition of the present invention is simple and easy to operate. It does not require professional technicians and large-scale instruments and equipment, has a low production cost, and is easy to achieve industrial development. DETAILED DESCRIPTION

[0034] In order to make the purpose, technical scheme and advantages of the embodiments of the present invention clearer, the technical scheme in the embodiments of the present invention will be described clearly and completely below. If the specific conditions are not specified in the embodiments, they are carried out according to conventional conditions or conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not specified, they are all conventional products that can be purchased commercially.

[0035] The present invention is further described below by means of specific examples. However, it should be understood that these examples are only used for more detailed description and should not be construed as limiting the present invention in any form.

[0036] The ginseng and maca used in the embodiments and comparative examples of the present invention are fresh raw materials purchased from the market, and green tea is purchased from the finished powder products that have been processed and ground from the market. The preparation methods of ginseng and maca are as follows: ginseng and maca are weighed with appropriate weights respectively, crushed into large particles by a grinder, and then placed in a multifunctional extraction tank respectively, and water is added to extract for 4 hours, and the filtrate is filtered through 100 mesh, and the filtered filtrate is concentrated under reduced pressure to a relative density of 1.06, and a dry powder is obtained by spray drying, and the dry powder is crushed and sieved through an 80-mesh sieve to obtain dry powders of each component for standby use.

[0037] Example 1 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 5 parts of ginseng powder and 6 parts of maca powder.

[0038] Example 2 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 1 part of ginseng powder and 8 parts of maca powder.

[0039] Example 3 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 7 parts of ginseng powder and 3 parts of maca powder.

[0040] Example 4 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 5 parts of ginseng powder and 6 parts of green tea powder.

[0041] Example 5 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 1 part of ginseng powder and 10 parts of green tea powder.

[0042] Example 6 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 7 parts of ginseng powder and 2 parts of green tea powder.

[0043] Example 7 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 5 parts of maca powder and 6 parts of green tea powder.

[0044] Example 8 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 3 parts of maca powder and 10 parts of green tea powder.

[0045] Example 9 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 4 parts of maca powder and 1 part of green tea powder.

[0046] Example 10 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 2.5 parts of ginseng powder, 2.5 parts of maca powder, and 6 parts of green tea powder.

[0047] Embodiment 11 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 1 part of ginseng powder, 1 part of maca powder, and 5 parts of green tea powder.

[0048] Example 12 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 4 parts of ginseng powder, 4 parts of maca powder, and 1 part of green tea powder.

[0049] Embodiment 13 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 3 parts of ginseng powder, 7 parts of maca powder, and 6 parts of green tea powder.

[0050] Embodiment 14 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 0.5 parts of ginseng powder, 0.5 parts of maca powder, and 1 part of green tea powder.

[0051] Embodiment 15 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 0.5 parts of ginseng powder, 0.5 parts of maca powder, and 10 parts of green tea powder.

[0052] Example 16 This embodiment is a composition for relieving physical fatigue, and its components are as follows: 5 parts of ginseng powder, 5 parts of maca powder, and 1 part of green tea powder.

[0053] Experimental Example 1 6 dpf wild-type AB strain zebrafish were selected, and then an equal amount of the composition in each embodiment was dissolved in water, and the zebrafish were placed in water containing food-derived ingredients at 27°C for 24 hours. The experimental treatments are shown in Table 1. After the zebrafish fatigue model was established by sodium sulfite induction for 30 minutes, the total movement distance of each zebrafish within 30 minutes was analyzed, the fatigue recovery rate was calculated, and the lactic acid and adenosine triphosphate (ATP) contents and related gene expression levels were determined.

[0054] The formula for calculating the recovery rate of zebrafish fry is as follows: Recovery rate = (swimming distance of treatment group - swimming distance of model group) / (swimming distance of control group - swimming distance of model group) × 100% The swimming distance of the control group was 2134.7 ± 178.6 mm. The recovery rate of zebrafish fry in each group was calculated according to the above formula. The specific results are as follows: Table 1 Effects of ginseng powder and maca powder on fatigue in zebrafish

[0055] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0056] As shown in Table 1, compared with the control group, the swimming distance of zebrafish in the model group decreased by 50.51%, indicating that sodium sulfite induced zebrafish fatigue and the fatigue model was successfully established. Red Bull, a recognized anti-fatigue health drink, was selected as the positive control group, and the swimming distance increased by 73.52% (p<0.01), and the swimming distance was significantly restored compared with the control group, indicating that Red Bull can significantly relieve physical fatigue.

[0057] When ginseng powder and maca powder were used alone, they increased the swimming distance of zebrafish (p<0.05), and the recovery rates were 40.6% and 42.3%, respectively, indicating that both have the potential to relieve physical fatigue. When ginseng powder and maca powder were used alone, they could increase the body's ATP content (p<0.05), reduce lactic acid content (p<0.05), and relieve physical fatigue. In addition, ginseng powder and maca powder also increased the expression of Nrf2, atp2b1, and g6pca genes (p<0.01), indicating that ginseng powder and maca powder can relieve physical fatigue by regulating the body's antioxidant, calcium ion metabolism, and energy metabolism pathways.

[0058] When ginseng powder and maca powder were used in a compound ratio of 5:6, 1:8 and 7:3, the swimming distance increased by 55.6%, 63.0% and 68.8% respectively (p<0.01), and the recovery rates were 49.8%, 56.5% and 61.7% respectively, and the corresponding CI values ​​were 0.43, 0.36 and 0.24 respectively, all less than 1, indicating that when the compound ratio was 1:8-7:3, ginseng powder and maca powder could synergistically relieve physical fatigue. When ginseng powder and maca powder were used in a compound ratio of 5:6, 1:8 and 7:3, the ATP content increased by 10.1%, 12.7% and 15.0% respectively (p<0.05), and the corresponding CI values ​​were 0.74, 0.88 and 0.32 respectively, all less than 1, indicating that the two can synergistically increase the ATP content and relieve fatigue. When ginseng powder and maca powder were mixed in a ratio of 5:6, 1:8 and 7:3, the lactic acid content decreased by 18.6%, 20.9% and 23.3%, respectively (p<0.01), and the corresponding CI values ​​were 0.77, 0.63 and 0.56, all less than 1, indicating that when the two are used in a ratio of 1:8~7:3, they can synergistically reduce lactic acid content and relieve physical fatigue.

[0059] When ginseng powder and maca powder were used alone, both increased the expression of Nrf2 gene (p<0.05), indicating that both can relieve physical fatigue by improving the body's antioxidant capacity. When ginseng powder and maca powder were used in a mixture of 5:6, 1:8 and 7:3, the expression of Nrf2 gene increased by 2.90, 2.96 and 3.03 times, respectively (p<0.01), and the corresponding CI values ​​were 0.37, 0.30 and 0.25, respectively, all less than 1, indicating that the combination of the two can synergistically regulate the body's antioxidant pathway to relieve physical fatigue. When ginseng powder and maca powder were used alone, they both increased the expression of atp2b1 gene (p<0.05). When the two were mixed at 5:6, 1:8 and 7:3, the expression of atp2b1 gene increased by 1.85, 1.92 and 2.05 times (p<0.01), and the CI values ​​were 0.66, 0.52 and 0.39, respectively, indicating that the combination can relieve physical fatigue by synergistically regulating the calcium ion metabolism pathway of the body. When ginseng powder and maca powder were used alone, they both increased the expression of g6pca gene (p<0.05). When the two were mixed at 5:6, 1:8 and 7:3, the expression of g6pca gene increased by 2.14, 2.25 and 2.33 times (p<0.01), and the CI values ​​were 0.77, 0.65 and 0.58, respectively, indicating that the combination can relieve physical fatigue by synergistically upregulating the energy metabolism pathway.

[0060] In summary, when ginseng powder and maca powder are mixed in a ratio of 1:8~7:3, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism pathways, synergistically increase the body's ATP content, accelerate lactic acid clearance, increase the swimming distance of zebrafish and improve the recovery rate, relieve physical fatigue, and the recovery effect at the optimal ratio is basically equivalent to that of Red Bull.

[0061] Experimental Example 2 The experimental method was the same as that of Experimental Example 1. The compound raw materials and their proportions are shown in Table 2. The swimming distance of the control group was 1863.4 ± 286.7 mm. The specific results are as follows: Table 2 Effects of ginseng powder and green tea powder on zebrafish fatigue

[0062] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0063] As shown in Table 2, when ginseng powder and green tea powder were used alone, they could increase the swimming distance of zebrafish (p<0.05), and the recovery rate was higher than 30%, which showed the potential to recover physical fatigue. When ginseng powder and green tea powder were used in a compound ratio of 5:6, 1:10 and 7:2, the swimming distance increased by 65.4%, 65.8% and 71.5% (p<0.01), respectively, and the recovery rate was 59.4%, 59.7% and 65.0%, respectively. The corresponding CI values ​​were 0.54, 0.44 and 0.39, respectively, which were all less than 1, indicating that when the compound ratio was 1:10~7:2, ginseng powder and green tea powder could synergistically relieve physical fatigue.

[0064] Ginseng powder can increase the body's ATP content (p<0.05) and relieve fatigue, while green tea powder has no significant difference, indicating that ginseng powder can relieve physical fatigue by increasing ATP content. However, when ginseng powder and green tea powder are used in combination at 5:6, 1:10 and 7:2, the ATP content increased by 13.1%, 12.0% and 17.1%, respectively (p<0.01), and the corresponding CI values ​​were 0.74, 0.88 and 0.32, respectively, indicating that when used in combination in the range of 1:10~7:2, the two can synergistically increase ATP content and relieve physical fatigue.

[0065] Both ginseng powder and green tea powder can reduce lactic acid content (p<0.05) and relieve physical fatigue. When the two are used in a compound mixture of 5:6, 1:10 and 7:2, the lactic acid content is reduced by 25.6%, 28.1% and 23.1%, respectively (p<0.05). The corresponding CI values ​​are 0.55, 0.49 and 0.37, all less than 1, indicating that ginseng powder and green tea powder can synergistically reduce lactic acid content and relieve physical fatigue when used in a compound mixture.

[0066] When ginseng powder and green tea powder were used alone, they both increased the expression of Nrf2 gene (p<0.05), indicating that both can relieve physical fatigue by improving the body's antioxidant capacity. When ginseng powder and maca powder were used in a mixture of 5:6, 1:10 and 7:2, the expression of Nrf2 gene increased by 2.92, 3.05 and 2.77 times, respectively (p<0.001), and the corresponding CI values ​​were 0.56, 0.47 and 0.61, respectively, all less than 1, indicating that the combination of the two can synergistically regulate the body's antioxidant pathway to relieve physical fatigue. When ginseng powder and green tea powder were used alone, they both increased the expression of atp2b1 gene (p<0.05). When the two were mixed at 5:6, 1:10 and 7:2, the expression of atp2b1 gene increased by 1.75, 1.58 and 1.80 times (p<0.01), and the CI values ​​were 0.29, 0.36 and 0.24, respectively, indicating that the combination can relieve physical fatigue by synergistically regulating the calcium ion metabolism pathway of the body. When ginseng powder and green tea powder were used alone, they both increased the expression of g6pca gene (p<0.05). When the two were mixed at 5:6, 1:10 and 7:2, the expression of g6pca gene increased by 1.94, 1.63 and 2.20 times (p<0.01), and the CI values ​​were 0.55, 0.61 and 0.33, respectively, indicating that the combination can relieve physical fatigue by synergistically upregulating the energy metabolism pathway.

[0067] In summary, when ginseng powder and green tea powder are mixed in a ratio of 1:10~7:2, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism, and synergistically increase the body's ATP content, accelerate lactic acid clearance, increase the swimming distance of zebrafish and improve the recovery rate, relieve physical fatigue, and the recovery effect at the optimal ratio is basically equivalent to that of Red Bull.

[0068] Experimental Example 3 The experimental method was the same as that of Experimental Example 1. The compound raw materials and their proportions are shown in Table 3. The swimming distance of the control group was 1624.8 ± 347.6 mm. The specific results are as follows: Table 3 Effects of maca powder and green tea powder on zebrafish fatigue

[0069] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0070] As shown in Table 3, when maca powder and green tea powder were used alone, they increased the swimming distance of zebrafish (p<0.05), and the recovery rate was above 30%, indicating that both have the potential to relieve physical fatigue. When maca powder and green tea powder were used in a compound ratio of 5:6, 3:10 and 4:1, the swimming distance increased by 55.7%, 61.8%, and 66.4%, respectively (p<0.01), and the recovery rates were 61.8%, 68.6%, and 73.7%, respectively. The corresponding CI values ​​were 0.55, 0.51, and 0.43, respectively, all less than 1, indicating that when the compound ratio was 3:10~4:1, maca powder and green tea powder could synergistically relieve physical fatigue.

[0071] Maca powder can increase the body's ATP content and relieve fatigue (p<0.05), while there is no significant difference with green tea powder, indicating that maca powder can relieve body fatigue by increasing ATP content. However, when maca powder and green tea powder are used in a compound mixture of 5:6, 3:10 and 4:1, the ATP content increased by 10.5%, 12.6% and 13.7%, respectively (p<0.01), and the corresponding CI values ​​were 0.56, 0.48 and 0.36, respectively, all less than 1, indicating that the two can synergistically increase ATP content and relieve physical fatigue.

[0072] Maca powder and green tea powder can also reduce the body's lactic acid content (p<0.05) and relieve physical fatigue. When the two are used in a compound mixture of 5:6, 3:10 and 4:1, the lactic acid content is reduced by 22.0%, 29.3% and 24.4%, respectively (p<0.01). The corresponding CI values ​​are 0.57, 0.31 and 0.48, all less than 1, indicating that when the two are used in a compound mixture of 3:10~4:1, they can synergistically reduce lactic acid content and relieve physical fatigue.

[0073] When maca powder and green tea powder were used alone, both increased the expression of Nrf2 gene (p<0.05), indicating that both can relieve physical fatigue by improving the body's antioxidant capacity. When maca powder and green tea powder were used in a mixture of 5:6, 3:10 and 4:1, the expression of Nrf2 gene increased by 3.01, 3.27 and 3.10 times, respectively (p<0.001), and the corresponding CI values ​​were 0.35, 0.21 and 0.29, respectively, all less than 1, indicating that the combination of the two can synergistically regulate the body's antioxidant pathway to relieve physical fatigue. When maca powder and green tea powder were used alone, they both increased the expression of atp2b1 gene (p<0.05). When the two were mixed at 5:6, 3:10 and 4:1, the expression of atp2b1 gene increased by 2.25, 2.36 and 2.44 times (p<0.01), and the CI values ​​were 0.55, 0.49 and 0.41, respectively, indicating that the two mixed together can relieve physical fatigue by synergistically regulating the calcium ion metabolism pathway of the body. When maca and green tea powder were used alone, they both increased the expression of g6pca gene (p<0.05). When the two were mixed at 5:6, 3:10 and 4:1, the expression of g6pca gene increased by 1.72, 1.83 and 1.89 times (p<0.01), and the CI values ​​were 0.59, 0.48 and 0.40, respectively, indicating that the two mixed together can relieve physical fatigue by synergistically upregulating the energy metabolism pathway.

[0074] In summary, when maca powder and green tea powder are mixed in a ratio of 3:10~4:1, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism, and synergistically increase the body's ATP content, accelerate lactic acid clearance, increase the swimming distance of zebrafish, improve recovery rate, relieve physical fatigue, and the recovery effect at the optimal ratio is basically equivalent to that of Red Bull.

[0075] Experimental Example 4 The experimental method was the same as that of Experimental Example 1. The compound raw materials and their proportions are shown in Table 3. The swimming distance of the control group was 1823.1 ± 251.3 mm. The specific results are as follows: Table 4 Effects of ginseng powder, maca powder and green tea powder on zebrafish fatigue

[0076] Note: The addition amount of each experimental group is the same; the symbol " / " indicates no corresponding value; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0077] As shown in Table 4, when ginseng powder, maca powder and green tea powder were used alone, they could increase the swimming distance of zebrafish (p<0.05), and the recovery rates were 44.6%, 48.4% and 32.7%, respectively, and they all had the potential to recover from sports fatigue. When ginseng powder + maca powder and green tea powder were used in a compound ratio of 5:6, 2:5 and 8:1, the swimming distance increased by 47.6%, 46.3% and 46.8% (p<0.01), and the recovery rates were 79.4%, 77.1% and 78.1%, respectively, and the corresponding CI values ​​were 0.25, 0.30 and 0.27, respectively, all less than 1, indicating that when the compound ratio was 2:5~8:1, ginseng powder, maca powder and green tea powder could synergistically relieve physical fatigue.

[0078] When ginseng powder and maca powder are used alone, they can increase ATP content (p<0.01) and relieve physical fatigue. There is no significant difference with green tea powder. However, when ginseng powder + maca powder and green tea powder are used in a compound mixture of 5:6, 2:5 and 8:1, the ATP content increases by 13.7%, 13.3% and 14.3% respectively (p<0.001), and the corresponding CI values ​​are 0.31, 0.37 and 0.35 respectively. This indicates that when the three are used in a compound mixture in the range of 2:5~8:1, they can synergistically increase ATP content and relieve physical fatigue.

[0079] Ginseng powder, maca powder and green tea powder can all reduce lactic acid content (p<0.05) and relieve physical fatigue. When the three are compounded in the ratio of 5:6, 2:5 and 8:1, the lactic acid content is reduced by 26.2%, 26.2% and 28.6%, respectively (p<0.001), and the corresponding CI values ​​are 0.38, 0.33 and 0.29, all less than 1, indicating that when ginseng powder, maca powder and green tea powder are compounded, they can synergistically reduce lactic acid content and relieve fatigue.

[0080] Ginseng powder, maca powder, and green tea powder can increase the expression of Nrf2, atp2b1, and g6pca genes when used alone (p<0.05), and can relieve physical fatigue by regulating the body's antioxidant, calcium ion metabolism, and energy metabolism pathways. When ginseng powder + maca powder and green tea powder were mixed at 5:6, 2:5, and 8:1, the expression of Nrf2 gene increased by 3.12, 3.17, and 3.26 times, respectively (p<0.001), and the corresponding CI values ​​were 0.29, 0.27, and 0.22, respectively, all less than 1, indicating that the three can synergistically regulate the body's antioxidant pathways to relieve physical fatigue after mixing. When ginseng powder + maca powder and green tea powder were mixed at 5:6, 2:5 and 8:1, the expression of atp2b1 gene increased by 2.14, 1.72 and 1.97 times (p<0.001), and the corresponding CI values ​​were 0.33, 0.48 and 0.39, all less than 1, indicating that the three can synergistically regulate the body's calcium ion metabolism and relieve physical fatigue. When ginseng powder + maca powder and green tea powder were mixed at 5:6, 2:5 and 8:1, the expression of g6pca gene increased by 2.34, 2.12 and 2.23 times (p<0.001), and the corresponding CI values ​​were 0.30, 0.39 and 0.35, all less than 1, indicating that the combination of the three can significantly synergistically regulate the body's energy metabolism pathway and relieve physical fatigue.

[0081] In summary, when ginseng powder + maca powder and green tea powder are compounded in a ratio of 2:5~8:1, they can synergistically regulate the body's antioxidant, calcium ion metabolism and energy metabolism pathways, and synergistically increase the body's ATP content, accelerate lactic acid clearance, increase the swimming distance of zebrafish and improve the recovery rate, and relieve physical fatigue.

[0082] Combined with the results of Experimental Examples 1 to 3, it was found that any two of ginseng powder, maca powder and green tea powder can synergistically relieve physical fatigue when combined, and the combination of the three can exert a stronger synergistic effect, and the recovery effect is slightly higher than that of Red Bull.

[0083] Experimental Example 5 The compound formula composed of ginseng powder, maca powder and green tea powder in different proportions has been shown to have a significant effect in relieving physical fatigue in zebrafish experiments. However, the structure of zebrafish is quite different from that of the human body, so the mouse fatigue model experiment was used to further verify the fatigue-relieving effect of the formula, providing reliable data support for the subsequent product development and use. According to the compound formula dosage combination verified in the zebrafish experiment, the conversion method of zebrafish to human dosage recorded in patent CN113496071A was used to convert the compound formula dosage in the zebrafish experiment into the mouse intake dosage, and the standard method of "Health Food Function Test and Evaluation Method (2023 Edition)" was strictly followed to verify the fatigue-relieving effect of the compound formula. The specific experimental process is as follows: Male C57 / 6J mice weighing approximately (20±2 g) were selected. The experimental treatments are shown in Table 5. They were gavaged continuously for 30 days under the same conditions. At the end of the experimental period, a weighted swimming test was performed on mice 30 min after gavage. The time from the start of swimming to the time when the head was completely submerged in water and could not swim out of the water for 5 s was recorded as the exhaustive swimming time of the mice. The contents of lactic acid, urea nitrogen, muscle glycogen and liver glycogen were measured, and the results are as follows.

[0084] Table 5 Effects of combined ginseng powder, maca powder and green tea powder on fatigue in mice

[0085] Note: The addition amount of each experimental group is the same; the statistical significance compared with the model group is recorded as * for p<0.05, ** for p<0.01, and *** for p<0.001.

[0086] As shown in Table 5, Red Bull, a recognized anti-fatigue health drink, was used as the positive control group, and the exhaustive swimming time of mice was significantly extended by 81.0% (p<0.001), indicating that Red Bull can significantly relieve the physical fatigue of mice. When ginseng powder + maca powder and green tea powder were used in a compound ratio of 1:1, 1:10 and 10:1, the exhaustive swimming time was extended by 77.8%, 64.7% and 87.0% respectively (p<0.01), indicating that when the compound ratio was 1:10~10:1, the formula had the effect of relieving the physical fatigue of mice.

[0087] When ginseng powder + maca powder and green tea powder were used in a 1:1, 1:10 and 10:1 ratio, the lactic acid content decreased by 35.8%, 37.3% and 33.6% (p<0.001), and the urea nitrogen content decreased by 21.7%, 17.7% and 18.2% (p<0.05), respectively, indicating that when the ratio was 1:10-10:1, physical fatigue could be relieved by reducing the lactic acid and urea nitrogen content of the body. In addition, when ginseng powder + maca powder and green tea powder were used in a 1:1, 1:10 and 10:1 ratio, the muscle glycogen content increased by 30.4%, 27.2% and 39.1% (p<0.05), and the liver glycogen content increased by 29.9%, 22.4% and 35.2% (p<0.01), respectively, indicating that after the three were mixed, physical fatigue could be relieved by increasing the body's glycogen level.

[0088] In summary, when ginseng powder + maca powder and green tea powder are compounded at a ratio of 1:10 to 10:1, it can significantly prolong the exhaustive swimming time of mice by reducing the accumulation of metabolic products in the body of mice and increasing the body's glycogen level, indicating that this formula can relieve physical fatigue in mice, and the fatigue recovery effect is slightly higher than that of Red Bull. So far, this formula has been proven to have a significant effect on relieving physical fatigue by the standard method of "Health Food Function Test and Evaluation Method (2023 Edition)".

[0089] Comparative Example 1 The components in this solution are: 40% polysaccharide wolfberry fruit powder.

[0090] Comparative Example 2 The components in this scheme are: hawthorn fruit powder.

[0091] Comparative Example 3 The components in this scheme are: Ganoderma lucidum spore powder.

[0092] Comparative Example 4 The components in this solution are: 4 parts of 40% polysaccharide wolfberry fruit powder and 5 parts of hawthorn fruit powder.

[0093] Comparative Example 5 The components in this scheme are: 4 parts of 40% polysaccharide wolfberry fruit powder and 3 parts of Ganoderma lucidum spore powder.

[0094] Comparative Example 6 The components in this scheme are: 5 parts of hawthorn fruit powder and 3 parts of ganoderma lucidum spore powder.

[0095] Comparative Example 7 The components in this solution are: 4 parts of 40% polysaccharide wolfberry fruit powder, 5 parts of hawthorn fruit powder and 3 parts of ganoderma lucidum spore powder.

[0096] The above comparative example was subjected to a zebrafish experiment according to the method of the above experimental example, and the test results are as follows: Table 6 Effect of comparison ratio on zebrafish fatigue

[0097] From the data in Table 6, it can be seen that 40% polysaccharide wolfberry fruit powder, hawthorn fruit powder and Ganoderma lucidum spore powder can extend the swimming distance on the basis of the model group, have a certain recovery rate, and show the ability to relieve physical fatigue. However, when the above three ingredients are combined, whether they are combined in pairs or in three combinations, the corresponding CI values ​​are 1.66, 1.47, 1.50 and 1.29, respectively, which are all greater than 1, proving that the various combinations of these three ingredients do not have a synergistic effect. It also shows that food-borne ingredients that have the effect of relieving physical fatigue alone do not necessarily have a synergistic effect when combined with each other.

[0098] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. A composition for relieving physical fatigue, characterized in that: The components of the composition are selected from at least two of ginseng, maca and green tea.

2. The composition according to claim 1, characterized in that The composition consists of the following components and their weight portions by weight: 1 to 7 parts of ginseng and 3 to 8 parts of maca.

3. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 1 to 7 parts of ginseng and 2 to 10 parts of green tea.

4. The composition according to claim 1, characterized in that The composition consists of the following components and their weight parts by weight: 2 to 10 parts of green tea and 3 to 8 parts of maca.

5. The composition according to claim 1, characterized in that The composition consists of the following components and their weight portions by weight: 1 to 7 parts of ginseng, 3 to 8 parts of maca, and 1 to 10 parts of green tea.

6. The composition according to claim 5, characterized in that The composition consists of the following components and their weight parts by weight: 3 parts of ginseng, 7 parts of maca, and 6 parts of green tea.

7. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for relieving physical fatigue.

8. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for improving ATP energy metabolism or reducing lactic acid.

9. Use of the composition according to any one of claims 1 to 6 in the preparation of a product for regulating the expression of Nrf2, atp2b1 and g6pca genes.

10. The use according to any one of claims 7 to 9, characterized in that: The types of the products include tablets, oral liquids, solid beverages, capsules, granules, powders, pills or tea bags.

Images