57 results about "Atp content" patented technology

The invention provides a biosensor for detecting adenosine triphosphate (ATP). The biosensor comprises exonuclease III, a triggering chain, an ATP exonuclease, a hairpin probe and a molecular beacon,wherein the ATP content in a solution can be detected through fluorescence; the excitation wavelength of the fluorescence is 486nm; the emission wavelength is 518nm; the detection ranges is 500-650nm.The biosensor is high in specificity, high in sensitivity, mild in reaction conditions, and high in repeatability; the detection method is simple and convenient to operate; the detection period is short; the main detection process is performed in homogeneous phase, so that the reaction speed is increased, and the complexity of operation is reduced.

The invention belongs to the field of electrochemical sensors, and particularly relates to a method for determining the ATP content on the basis of magnetic bead separation and a DNA marker gold nanoparticle probe. The method comprises the steps that firstly, carboxylation magnetic beads (MB) are combined with amino-modified ATP aptamer DAN1 to generate an MB-DNA1 compound; CV aptamer DNA2 modified with SH and ATP aptamer complementary chain DNA3 are combined with nanogold, and CV is added to generate a probe CV / DNA2 / DNA3 / AuNPs; the MB-DNA1 compound is reacted with the probe, and magnetic beads modified with the probe are generated through the combination and complementation action of DNA1 and DNA3; a sample solution containing ATP is added into a magnetic bead solution modified with the probe, magnetic separation is conducted, and supernatant is taken; then the supernatant is dropwise added on an electrode modified with gold nanoparticles. The obtained electrode serves as a working electrode, and the working electrode, a reference electrode and an indicating electrode are inserted into an electrolyte solution to be subjected to electrochemical determination. Determination of the ATP content is achieved according to the intensity of an electrochemical signal.

The invention discloses a sanitary quality locale rapid detection device and a detection method, the device comprises an auto sampling element, a photoelectricity conversion element and a control, operation and storage display circuit element. The method comprises that: the detected parameters are selected, based on an ATP bioluminescence theory, the detected sample and three groups detection reagents of a body cell lysates agent, a bacterial cell lysates agent and a luciferase-luciferase luminescence reagent are added to a sample pool, each reagent is provided with an auto sample adding device connected with the sample pool, the auto sample adding device controls to inject different reagents to the sample pool according to the detection requirement, the sample surface cleanliness condition is gained by measuring the total ATP content, the bacteria quantity in the sample is quantitatively detected by measuring the ATP content in the bacteria cell. Compared with the traditional device, the detection speed of the invention is fast, the detection time is less than 30 minutes, the degree of automation is high, and the maneuverability is good, the invention can be applicable to the locale rapid detection of health quality in the food, cosmetics, medical, environmental, and other fields.

The present invention discloses a portable adenosine triphosphate fluorescence detection device comprising a housing consisting of an upper housing and a lower housing, a cover capable of opening / closing and deposited above the housing, a liquid crystal window and a keyboard which are deposited at the upper housing acting as a panel of the device; a circuit board used for performing a fluorescence detection and mounted inside the housing, with a liquid crystal screen, a photodiode, a shielding case and a light trap metal block being mounted thereon, wherein the photodiode is arranged inside the light trap metal block with its the other terminal being connected to a test-tube repository, and a first microswitch and a second microswitch are also mounted at the circuit board. The control circuit comprises a fluorescence detection module, a data acquisition module, a human-computer interface, a power supply module, a control module and the like. Said fluorescence detection module is used for detecting the intensity of fluorescence generated by a reaction of ATP in living microorganism body and fluoresceins and luciferases in the detection reagent, and the data acquisition module is used for converted the fluorescence intensity detected into a digital quantity to ascertain the ATP content, then the presence and content of microorganism is capable of being indirectly ascertained by controlling system analysis and processing and saving data.

The embodiment of the invention discloses a detection method of ATP (Adenosine Triphosphate) content and an ATP aptamer sensor. The detection method of the ATP content comprises the following steps of: providing a gold electrode the surface of which is fixed with a DNA single strand, wherein the DNA single-stranded sequence is shown as SEQ ID No.1; providing part of DNA double strands, wherein the tail end of the first strand 3' in the partial DNA double strands is shown as SEQ ID No.2 and the tail end of the strand 5' is complementary to the second strand; immersing the gold electrode in the mixed liquor of the partial DNA double strands and a sample to be tested; by using Ru(phen)3<2+>, [Ru(bpy)2dppz]<2+> or [Ru(phen)2(dppz)]<2+> as an electrochemical luminescence probe, performing ECL (Electrochemiluminescence) detection on the gold electrode. By using aromatic ring with larger ruthenium compound ligand, the invention has the performance of embedding the DNA double-stranded structure and realizes detection of the ATP; and besides, the detection method does not need to use chemical marks and is simple.

The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that determine the proliferative status of isolated target cell populations. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The present invention further relates to kits that provide reagent mixes and instructions for high-throughput assays methods for screening compounds that may modulate the proliferative status of a target cell population. The kits of the present invention and methods therein described may be used for determining the proliferative status of any isolated cell line or type. The kits and methods of the present invention address the need for rapid assays that determine the proliferative status of isolated hematopoietic stem and progenitor cells and of subpopulations of differentiated cells thereof.

The invention discloses a high-flux bacterial colony testing chip, a high-flux bacterial colony testing system and a high-flux bacterial colony testing method. The testing chip comprises a base material and at least one testing unit distributed on the base material, wherein each testing unit comprises a first mixed reaction tank, a second mixed reaction tank and a testing pool which are sequentially arranged along the first direction, the first mixed reaction tank is provided with an ATP extracting solution sample inlet and a bacteria solution sample inlet, the second mixed reaction tank is provided with an enzyme solution sample inlet, an S-shaped microchannel valve is arranged between the first mixed reaction tank and the second mixed reaction tank, and the second mixed reaction tank is communicated with the testing pool. The ATP concentration is determined by testing the fluorescence intensity, good linear relation is available between the ATP content and the microbe quantity, and the total number of the bacterial colony can be calculated by utilizing the principle that the ATP content is in direct proportion to the bacteria quantity. With the adoption of the micro-fluidic chip, the man-made interference factors are reduced, the attenuation of the fluorescence intensity during the testing process is reduced, and the testing accuracy is improved.

The invention relates to a method for determining ATP. The method is used for determining ATP content in bacteria-containing water body, soil, sludge, etc. The method comprises the following steps of a) preparing a to-be-measured solution of a sample; b) adding a BAB solution in the to-be-measured solution; c) sucking up a filtered filtrate and putting into a filtering cuvette with luciferase added therein already; and d) performing an ATP determination by using a Profile-1 biological luminescent instrument. Compared with a conventional method, the method provided by the invention is superior to a standard analysis method installed in the Profile-1 biological luminescent instrument, is particularly suitable for determining ATP in the soil, and makes up disadvantages of the standard analysis method installed in the Profile-1 biological luminescent instrument.

The invention discloses a method for efficiently protecting the mitochondrial function of vitrified frozen bovine oocytes. According to the method, 10-9M melatonin (MT) is added into a bovine oocyte in vitro maturation medium and a vitrification solution. There is no significant difference between bovine oocytes frozen by using this method and fresh oocytes with respect to ATP content and in vitro development. Application of the method of the invention can improve the developmental capacity and application scope of frozen oocytes. The method of the invention is simple in execution and low in cost, and will play a great role in cryopreservation of bovine oocyte.

The invention relates to preparation and application of ATP (adenosine triphosphate) near-infrared nanometer fluorescence probe. The fluorescence probe is structurally formed by nano-level zeolite imidazole frameworks (ZIF-90) and rhodamine-based near-infrared fluorophores coated inside the nano-level zeolite imidazole frameworks. The invention provides a preparation method for synthesizing the fluorescence probe by using 2-(4-diethylamino-2-hydroxybenzoyl) benzoic acid, trimethyl-2-indoline, zinc acetate dehydrate, imidazole-2-formaldehyde and the like as raw materials. The fluorescence probeis an ATP near-infrared fluorescence probe based on ZIF-90. Firstly, the fluorescence probe is simple in synthesis method and can generate 72 times of fluorescence enhancement for ATP; secondly, thefluorescence probe has relatively ideal selectivity for the ATP and cannot be obviously interfered by common ions in other nucleotides and living bodies; moreover, the fluorescence probe is rapid to react with the ATP, and response time is within 400s; and in addition, the fluorescence probe has long near-infrared emission, and is applied to detection on ATP content in living cells.

The present invention relates generally to kits that provide reagent mixes and instructions for the use thereof, in performing high-throughput assay methods that provide a method of screening compounds for cytotoxicity or other effects on target cell populations of the lymphohematopoietic system, including specific lineages. The methods measure the luminescent output derived from the intracellular ATP content of incubated target cells, and correlate the luminescence with the proliferative status of the cells. The methods may be used to predict the effect of virtually any compound on the lymphohematopoietic system and may be performed on multiple species simultaneously, thereby providing valuable information regarding potential cytotoxicity prior to preclinical studies and especially, patient clinical trials. The methods also provide the ability to screen compounds early in the drug development profile.

The invention discloses a mitochondria injection and application thereof. The mitochondria injection is an injectable solution, an emulsion or a suspension prepared by adding solvents or other auxiliaries into mitochondria which is extracted from tissues or cells of animals, plants and microorganisms through performing germfree operation and the gradient centrifugation technology and serves as a raw material. The prepared mitochondria injection can be applied to local drug delivery and intracardial injection. After the injection is applied, the ATP content in the tissues can be increased, and the activity of the cells can be enhanced; the mitochondria injection is used for treating Parkinson's disease relevant to mitochondria damage and diseases such as liver injury induced by medicines, so that an effective and innovative treating method is provided for mitochondria-associated diseases.

The invention discloses a skin cell vitality promoting function of a saussurea involucrata extract and applications of the extract in preparations for external use for skin, especially in cosmetics, and particularly discloses applications of the saussurea involucrata extract in epidermic cell mitochondrion activity enhancement and / or epidermic cell ATP content increasing.

The invention relates to a method and a detection kit for rapidly detecting the number of microorganisms using ATP bioluminescence method. The rapid detection kit includes luciferase and its protective agent, D-luciferin, standard ATP, luminescent detection buffer, acellular ATP remover, microbial cell ATP extractant and other reagents. The standard method for using this detection kit to quickly determine the number of microorganisms is to make an ATP standard curve in a selected bioluminescence instrument and a selected system, and obtain a linear regression equation after taking the logarithm. After removing non-target ATP and microbial The sample solution extracted by ATP is detected in the same instrument and system with the same batch of enzymes for ATP bioluminescence detection, and a blank control is made at the same time to obtain the CPM or RLU value of the sample and the blank, and the net relative luminescence value after removing the blank is taken as logarithm Finally, the ATP concentration of the sample is obtained through the established regression equation. According to the ATP content of microorganisms such as bacteria, yeast, mold and unicellular microalgae, it can be converted into the number of cells of bacteria, yeast, mold spores or unicellular microalgae, or only calculated Equivalent to the number of bacteria.

The invention provides a method for promoting the survival of injured neurons by virtue of mitochondria transplantation. The method comprises the following steps: (1) carrying out trypsinization centrifuge on P1-P3-generation mesenchymal stem cells, and counting the number of the cells; (2) separating by virtue of a mitochondria separation agent to obtain mitochondria, and carrying out protein quantification and ATP content determination; and (3) preparing suspension from the mitochondria obtained in the step (2), and transplanting the suspension to a neuron injury region to contact with the neuron injury region. Compared with the transplantation of mesenchymal stem cells, the method has the advantages that the allogeneic immunogenicity problem is avoided, the implantation problem of chromosomes of allosome-derived stem cell nucleuses is avoided, and the regeneration of neuronal cells is promoted.

The invention discloses an ATP content determination kit and a method thereof. The kit comprises a reagent I prepared by dissolving concentrated perchloric acid in distilled water, a reagent II prepared by dissolving sodium hydroxide in distilled water, a reagent III prepared from sodium phosphate dibasic dodecahydrate, a reagent IV prepared from sodium dihydrogen phosphate dihydrate and a reagent V prepared from ATP. The method improves the extraction process of ATP and is capable of removing influence of impurities in a sample by firstly adding an acidic extraction solution into the sample for fully extracting and then adding an alkaline extraction solution for neutralizing. The minimum detection limit of the method reaches up to 0.1Mu mol / mL; compared with a conventional spectrophotometric method, the minimum detection limit is significantly improved. The reagent components of the kit only comprise absolute methanol, disodium hydrogen phosphate and sodium dihydrogen phosphate; the detection cost is greatly reduced. The method is qualitative and quantitative in through-peak area and retention time; the detection accuracy is significantly improved.

The invention discloses an adenosine-triphosphate purity detection method. The adenosine-triphosphate purity detection method includes the following steps that moisture in ATP standard substances is subjected to quantitative detection to obtain the content of the moisture, inorganic impurities in the ATP standard substances are subjected to quantitative detection to obtain the content of the inorganic impurities, ATP is detected with the high performance liquid chromatography, the content of the ATP is calculated with the area normalization method, and then the purity of the adenosine triphosphate in the ATP standard substances is calculated with the following formula; the purity of the adenosine triphosphate is obtained in the mode that the content of the moisture and the content of the inorganic impurities are subtracted from 100%, and the result is multiplied by the ATP content. The adenosine-triphosphate purity detection method is more accurate and reliable, the purity of the ATP standard substances can be accurately and stably measured, the detection method is applied to the field of bacterium rapid detection, quality control of the results of calibration of related instruments and verification and detection of the detection method are guaranteed, and standardization work of the method is advanced.

The invention discloses a method for extracting SOD, CAT, NADH oxidase and ATP in a white-rot fungi composite adsorbent simultaneously. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously comprises the following steps: adding the white-rot fungi composite adsorbent into a CaCl2 solution to prepare small balls with white-rot fungi being embedded, and carrying out constant temperature shaking culture on the small balls with the white-rot fungi being embedded to obtain a culture solution; adding Cd<2+> into the culture solution for carrying out stress culture, then taking out the small balls with the white-rot fungi being embedded, and carrying out liquid nitrogen grinding to obtain a grinding sample; and adding phosphate buffer into the grinding sample, carrying out ultrasonic treatment in an ice bath, centrifuging to obtain a compound of SOD, CAT, NADH oxidase and ATP. The method for extracting SOD, CAT, NADH oxidase and ATP in the white-rot fungi composite adsorbent simultaneously has the advantages that heavy metal stress is adopted for improving enzyme activity of SOD, CAT and NADH oxidase and increasing ATP content, a cell breakage effect is good and extraction efficiency is high.

The invention relates to a kit for determining ATP content in trace amounts of sample and application thereof. The kit comprises a solution I which is a mixture of TrisHCl and bovine serum albumin; a solution II which is a sodium hydroxide solution; a solution III which is a mixture of hydrochloric acid and the TrisHCl; a reagent I comprising the TrisHCl, the bovine serum albumin, magnesium chloride, dithiothreitol, D-glucose, nicotinamide adenine dinucleotide phosphate, hexokinase and colourless glucose-6-phosphate dehydrogenase; a reagent II comprising Imidapril HCl, the bovine serum albumin, alpha-ketoglutaric acid monosodium salt, sodium hydroxide, ammonium acetate, adenosine diphosphate, glucose-6-phosphoric acid, glutamate dehydrogenase and the colourless glucose-6-phosphate dehydrogenase; a reagent III comprising the Imidapril HCl, ethylenediaminetetraacetic acid, the magnesium chloride, the ammonium acetate, NADP and glucose 6-phosphate dehydrogenase. The kit is simple to operate, and the determination result is more accurate.

The invention discloses a method for improving the antioxidant capacity of yeast and belongs to the field of biological engineering. According to the method, H2O2 with a proper concentration is used for stimulating yeast cells and a 96-pore plate mini-type liquid culture method is used for screening strains with rapid growth speed and high activity; compared with starting strains, the obtained strains have the advantages that the intracellular ROS (Reactive Oxygen Species) accumulation level is reduced by 30 percent; except one strain of fruit wine yeast, the increasing amplitude of the intracellular ATP (Adenosine Triphosphate) content exceeds 50 percent. The method disclosed by the invention can be used for effectively reducing the intracellular ROS accumulation amount of the yeast and increasing the intracellular ATP content, so that the aim of improving the antioxidant capacity of yeast strains is realized.

The invention provides a rapid evaluation method of an oil field reinjection water sterilizing agent. The rapid evaluation method comprises the following steps: 1, filtering a water sample to be detected, and intercepting microorganisms in the water sample to be detected; 2, fetching a lysis solution to lyse microorganisms in the water sample to be detected, and putting into a sample tube with a buffer solution; 3, connecting an ATP detector and a computer; 4, fetching an ATP standard solution, and calibrating luciferase activity by using the ATP detector; 5, fetching the solution in the sample tube in the step 2, and detecting the ATP content of the sample. By the rapid evaluation method of the oil field reinjection water sterilizing agent, the evaluation efficiency of the oil field reinjection water sterilizing agent can be greatly improved and the detection time can be effectively shortened.

A microorganism measuring system set control reference values with higher accuracy than ever. Based on results of repeated CFU countings and results of repeated ATP measurements, a microorganism measuring system obtains a probability density function of ATP measured values in a normal condition; determines an alert reference value at which a desired probability of false positives is equal to or less than a probability, and an action reference value at which a desired probability of false negatives is equal to or less than a probability; and thereby controls the ATP measured values. For this reason, the microorganism measuring system can set the control reference values with higher accuracy than ever, without being influenced by an error in the conversion of ATP contents into CFU counts. Accordingly, the microorganism measuring system can achieve the microbiological control with desired control accuracy

There is described an isolated 3-dimensional liver spheroid wherein said spheroid has: increased ATP content as compared to a 3-dimensional liver spheroid cultured in Complete William E medium alone;the same or increased activity of cytochrome P450 1A1 and cytochrome P450 1B1 as compared to a 3-dimensional liver spheroid cultured in Complete William E medium alone; and increased albumin secretionas compared to a 3-dimensional liver spheroid cultured in William E medium alone.

The invention discloses application of UL43 protein in preparing a medicine for preventing and treating cellular mitochondrial dysfunction and a medicine for preventing and treating metabolic syndrome. It is experimentally found that the UL43 protein can be endocytosed into a cell from a host cell membrane and localized on mitochondria of a host cell, meanwhile, the UL43 protein can enhance activity of PFK-1 and PK enzyme in glycolysis pathway to produce a large amount of pyruvate entering the mitochondria, so that the TCA circulation and oxidative phosphorylation process are further activated, thus the intracellular ATP content is increased, and treating for mitochondrial dysfunction and metabolic syndrome diseases is achieved.

The invention discloses a method for rapidly detecting the content of ATP of a plant leaf. The method comprises the following steps: (1) utilizing a spectrometer to collect the spectral signals of plant leaves, which have been stored at a temperature of 0 to 25 DEG C for different days, in a waveband of 221.99 to 1019.78 nm, and preprocessing the spectral signals; (2) using a bioluminescence detection method to measure the ATP contents of the plant leaves; (3) subjecting the preprocessed spectral signals to dimensionality reduction, extracting the characteristic wavelengths, and taking the ATP contents as the output and the strengths of spectral signals corresponding to the characteristic wavelengths as the input to establish a multiple linear regression model; and (4) measuring the strengths of spectral signals of plant leaves at a temperature of 0 to 25 DEG C at the characteristic wavelengths, and substituting the strength value into the multiple linear regression model to calculate the ATP content of the plant leaves through. The provided method has the advantages of simple detection process, high work efficiency, and reliable detection results.

The invention relates to the field of food quality analysis, and in particular relates to a method for detecting the freshness of aquatic products. The method comprises the steps of sample pretreatment; mixing metal organic frame / copper nanocluster, aquatic product supernate and Tris-HCl buffer solution and incubating, and determining the concentration range of the ATP through measuring the fluorescence-emission intensity, and putting the intensity value into a linear equation. The composite material compounded by the invention is low toxicity, pollution-free, low in cost, simple and easy to acquire, and has the advantages of high sensitivity, strong antijamming capability, simple and easy to operate and capable of achieving quick detection when being applied to detecting the ATP; by usingthe method, under the optimized best experiment condition, the detection on the ATP content in the aquatic product can be accurately and quickly achieved, and the content can be used as the assessment criteria for the freshness of the aquatic product.

The invention discloses an ATP analysis method based on ratio inorganic mass spectrometry (ICPMS) detection. First, the ATP aptamer DNA1 is chemically combined with the magnetic beads through an amide reaction, and a small piece of DNA2 complementary to DNA2 is combined with the gold nanoparticles through the formation of AU‑S bonds. Second, the two complexes were added to the reaction system in a specific ratio to form a core‑satellite structure. When ATP was added to the system, the specific binding of the aptamer to ATP resulted in the release of satellite signals (AuNPs). As the amount of ATP increased, the AuNPs released in the supernatant increased, as detected by ICPMS of the supernatant 197 Au / 115 The In signal ratio becomes higher and higher. because 197 Au / 115 The In signal ratio is related to the corresponding ATP amount, and the present invention passes 197 Au / 115 The signal ratio of In enables the determination of ATP content.

The method of non-radioactive discrimination of membrane protein transport function, recording the peak area displayed by ADP and ATP under different conditions, according to the peak area of ​​ADP and ATP to obtain their relative amount after the transport reaction, combined with different time points of transport reaction to obtain ADP / ATP If the ATP content tends to decrease and the relative ADP content tends to increase as the reaction time increases, it can be judged that the membrane protein has the function of transporting ADP / ATP. This method is superior to the isotope tracer method used to study the transport function of membrane proteins, mainly because of its safety, simplicity, and low cost.

The invention discloses a method for reducing stress and improving meat quality of live pigs by using a protein acetylation inhibitor. The method comprises the following steps that curcumin is injected in an abdominal cavity half an hour to 1 hour before slaughtering with the dosage of 15-20mg / kg, so that the stress level of the live pigs can be obviously reduced, and stress level reduction is shown as the reduction of the concentrations of creatine kinase, lactic dehydrogenase, cortisol and heat shock protein in blood; and the meat quality of main cut meat such as shoulder meat, longissimus dorsi, psoas major, musculi semimembranosus and semitendinosus musele is obviously improved, and the meat quality improvement shows that the activity of creatine kinase and hexokinase is reduced, the activity of pyruvate kinase is increased, the glycogen and ATP content is high, the lactic acid content is low, the muscle pH reduction is slowed down, and the PSE meat occurrence rate is reduced. The method not only can reduce the animal stress, but also can improve the meat quality.