Method for determining ATP

A solution, a technology of benzalkonium bromide solution, applied in the field of determination of ATP content, achieves good application prospects and practical value, and the effect of improving sensitivity and accuracy
CN102818801AInactive Publication Date: 2012-12-12TONGJI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
TONGJI UNIV
Publication Date
2012-12-12
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a method for determining ATP. The method is used for determining ATP content in bacteria-containing water body, soil, sludge, etc. The method comprises the following steps of a) preparing a to-be-measured solution of a sample; b) adding a BAB solution in the to-be-measured solution; c) sucking up a filtered filtrate and putting into a filtering cuvette with luciferase added therein already; and d) performing an ATP determination by using a Profile-1 biological luminescent instrument. Compared with a conventional method, the method provided by the invention is superior to a standard analysis method installed in the Profile-1 biological luminescent instrument, is particularly suitable for determining ATP in the soil, and makes up disadvantages of the standard analysis method installed in the Profile-1 biological luminescent instrument.
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Description

technical field

[0001] The present invention relates to a method for measuring ATP content, in particular to a method for measuring ATP content in bacteria-containing water, soil, and sludge using a Profile-1 bioluminescence instrument, and indirectly expressing microorganisms in soil by measuring ATP content Quantitative method. Background technique

[0002] ATP (Adenosine Triphosphate) is a specific high-energy phosphate compound in organisms. The ATP content in normal organism cells is relatively stable. Therefore, the ratio of ATP content to active microbial mass can be considered as a constant. Therefore, ATP can be used as an indicator to characterize the active microbial biomass. The ATP bioluminescence method uses ATP to react with luciferin-luciferase to generate photons, and then detects the luminescence value with a fluorescence detector to obtain the amount of ATP. Its reaction process is as follows:

[0003]

[0004]

[0005] At present, there are mai...

Claims

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