7 results about "Luciferase Gene" patented technology

The invention provides a chicken PGC reporter gene cell strain for detecting the biological activity of FGF2 as well as a construction method and application of the chicken PGC reporter gene cell strain, and belongs to the technical field of biological activity detection. The cell strain is a recombinant cell which is stably integrated with a reporter gene expression cassette responding to an FGF signal channel in a chicken PGC genome; the expression cassette comprises a serum response element (SRE), a minimum promoter and a reporter gene (such as a luciferase gene). The construction method comprises the following steps: introducing a donor vector containing the elements into chicken PGC by utilizing a PiggyBac transposon system, and screening to obtain a stably expressed cell strain. The cell strain provided by the invention retains endogenous signal channel characteristics of chicken PGC, and can specifically and highly sensitively respond to FGF2 stimulation. The method can be used for biological activity quantitative determination and thermal stability evaluation of the FGF2 protein and the mutant thereof, quality control of culture medium components and screening of an FGF signal channel regulator.

This invention discloses an attenuated chikungunya virus expressing a nanoluciferase gene, the nucleotide sequence of which is shown in SEQ ID NO:1. It is constructed by attenuating the virulence of the chikungunya virus LR2006-OPY1 strain by deleting 61 amino acids from positions 323 to 383 of the nsP3 protein and inserting a nanoluciferase gene after position 490 of the nsP3 protein. This virus model maintains similar growth characteristics to the wild-type virus but with significantly improved safety. Viral replication can be monitored in real time by detecting luciferase activity, making it suitable for drug screening at both cellular and animal levels. This provides an efficient and safe technical platform for the development of antiviral drugs against chikungunya virus.

The invention provides a high-sensitivity in-vitro pyrogen detection method, which is characterized in that on the basis of an in-vitro pyrogen detection method (reporter gene method), a transgenic cell which expresses a Toll-like receptor and transfects an NF-kB reaction element and a luciferase gene is used for detecting a pyrogen, the pyrogen is combined with the Toll-like receptor on the surface of the cell to activate an NF-kB signal channel, and the NF-kB reaction element and the luciferase gene are converted into the NF-kB signal channel. Therefore, the expression of luciferase is activated.

The present invention concerns bioluminescent plants and methods for producing them. Among said methods, the invention relates, in particular, to a method of introducing luciferase genes and luciferin biosynthesis genes into the chloroplasts of a plant. In addition, the invention concerns a method of producing light comprising adding chemical compounds to a culture medium of the plant according to the invention.

The invention discloses a cell model for detecting monocyte and macrophage IL-1beta response level by using secretory luciferase, and belongs to the technical field of cell models. The technical problem to be solved is that in the prior art, a method capable of tracking response levels of mononuclear and macrophage factors IL-1beta in a real-time and non-invasive manner under different stimuli is lacked. According to the technical scheme, the cell model for detecting the IL-1beta response level of the mononuclear and macrophages is provided, and a preparation method of the cell model comprises the steps that an IL-1beta promoter is placed on the upstream of a secretory luciferase gene, lentivirus plasmids are introduced, and recombinant lentivirus plasmids are obtained; co-transfecting a host cell with the recombinant lentivirus plasmid and a packaging plasmid to prepare a recombinant lentivirus; and introducing the recombinant lentivirus into the THP-1 cell to obtain the THP-1 cell for expressing luciferase.

This invention relates to an infectious clone of CA16 virus carrying the luciferase gene Nluc, its construction, and its applications. The P1 region of the CA16 genome from strain OP293089 was constructed into pcDNA, and the P2 and P3 regions were constructed into the pUC57 vector. The P1 and P2+P3 regions were recombined with the pSVA vector to construct an infectious CA16 clone. The Nluc luciferase reporter gene was then introduced to construct the pSVA-CA16-Nluc infectious clone. After rescuing the CA16-Nluc virus, its activity was experimentally verified to be similar to that of the parent virus. After continuous passage in Vero cells, the genetic stability of CA16-Nluc in in vitro cell culture was experimentally verified. Furthermore, this infectious clone can be used for high-throughput drug screening and has wide applications in basic research on viral replication and pathogenesis mechanisms, high-throughput drug screening, and neutralizing antibody screening.

Method for producing a spontaneous metastasis model is disclosed herein. The models, according to various embodiments herein, achieve a higher metastasis incidence rate, in an instance about 100% for mesenteric lymph node (mLN) invasion and about 80 to 90% for secondary organs. The models are kinetic model having a luciferase-based expression system that provide more statistically significant and robust data. The embodiments herein further include a method for producing the model for metastasis, comprising transplanting recombinant carcinoma cells having increased invasiveness and decreased tumorigenic properties and luciferase gene expression system, in one or more orthotopic positions in an animal.