This disclosure relates to the field of
biotechnology, specifically to a 5'UTR sequence for promoting mRNA translation and its uses. This disclosure provides a 5'UTR sequence comprising the
nucleotide sequence shown in any one of SEQ ID NO: 1-6 or its complementary sequence, or a
nucleotide sequence having at least 80% homology with the
nucleotide sequence shown in any one of SEQ ID NO: 1-6 or its complementary sequence. This disclosure provides a novel
RNA molecule design scheme that, through rational optimization of the
nucleic acid sequence of functional regions, achieves the
advantage of optimized translation, making this
RNA molecule promising for broad applications in
gene therapy, vaccine development, and other fields.