9 results about "Virus suspension" patented technology

Particles are described that include a virus encapsulated in an amorphous silica shell, where the amorphous silica shell is deposited directly around the surface of the virus. Also described is a method of making the virus encapsulated in an amorphous silica shell, comprising concentrating or purifying the virus, suspending the virus in a buffer, hydrolyzing a silica precursor, directly contacting the hydrolyzed silica precursor with the surface of the virus in the buffer, and encapsulating the virus in the amorphous silica shell.

The invention belongs to the technical field of agricultural microbial preparations. The rice thuringiensis granule suspending agent comprises a bacillus thuringiensis concentrated solution and a rice leaf folder granule virus suspension according to a volume ratio of 1000: (1-2), in the bacillus thuringiensis concentrated solution, the biological value of bacillus thuringiensis is greater than or equal to 16000 IU / ml; in the rice leaf roller granulovirus suspension, the rice leaf roller granulovirus content is greater than or equal to 100000 OB / ml. The invention is used for solving the technical problem that the existing common biopesticide dosage form cannot meet the unmanned flight prevention requirement. The invention further discloses a preparation method of the rice thuryun longitudinal particle suspending agent. The invention further discloses an application method of the rice thuryun longitudinal particle suspending agent.

The invention discloses an organoid gene editing method and application thereof in constructing a disease model. The organ-like gene editing method comprises the following steps: fixing an organ-like; under a microscope, injecting the virus suspension into the organoid; the organoid after injection is cultured; wherein the adopted virus is an adenovirus packaged with an hCas9-P2A-EGFP fusion protein coding sequence driven by a CBh promoter and a gRNA coding sequence driven by two U6 promoters. According to the method, gene editing is directly carried out on a complete organoid based on the CRISPR-Cas9 technology, the organoid gene editing method which is efficient, low in damage and easy and convenient to operate is provided, the related disease model can be rapidly constructed, the occurrence mechanism of human diseases can be simulated, and the method has a good application prospect. Therefore, more stable and reliable technical support and ideal experimental subjects are provided for etiological research, drug research and development, therapeutic schedule verification and the like.

The present invention discloses an energy-saving and clean environmental control and fuel tank inerting coupling system based on heat recovery, belonging to the field of aircraft airborne electromechanical systems. The present invention utilizes the recovered energy to drive a high-boost-ratio air centrifugal compressor to suck in external ram air and compress, heat up and boost the pressure to meet the requirements of the environmental control system for refrigeration, cabin pressurization, etc.; uses the cabin exhaust gas as the cold source of the regenerative heat exchanger to pre-cool the high-temperature and high-pressure gas; uses the cooling turbine to drive the compressor to boost the pressure of the oxygen-rich gas separated by the fuel tank inerting system to achieve cabin oxygenation. The present invention adopts a coupling method to fully recover and utilize the heat of the thermal protection system, without bleeding air from the engine, reducing the aircraft's compensation loss and improving the energy utilization rate; fully recovers and utilizes the waste gas discharged from the cabin, reduces the amount of ram air, reduces the aircraft drag and lightens the weight of the heat exchanger; the environmental control system adopts a three-stage self-cleaning filter, which can effectively prevent the deposition of virus suspensions and prevent the spread of epidemics.

A first method of analyzing recombinant viruses is provided. The method comprises infusing a sample of recombinant viruses into a charge-detection mass spectrometer, the spectrometer comprising an electrospray needle that is maintained at a first temperature for a first period of time; detecting a first analyte during the first period of time; maintaining the electrospray needle at a second temperature higher than the first temperature for a second period of time; and detecting the first analyte during the second period of time. A second method of using the first method to compare stability of two or more distinct viral suspensions is also provided.

The invention discloses a construction method and system of a herpes simplex keratitis infection model. The method comprises the following steps: fixing a mouse in a mild anesthesia state and enabling eyeballs of the mouse to protrude as much as possible, sucking an HSV-1 virus suspension by using a microsyringe, obliquely inserting a needle from the cornea edge close to the sclera, keeping the inclined surface of the needle upward, slowly penetrating through the cornea epithelium to enter a target position of a matrix layer, and finally obtaining the HSV-1 virus suspension. The needle head is tightly attached to the cornea plane in the injection process; after injection is completed, the needle is slowly withdrawn; in the needle inserting and withdrawing processes, real-time observation is carried out through a stereoscopic microscope to ensure that the needle tip is kept away from the crystalline lens. According to the method, the stable and reliable HSK model can be established under the low virus titer (1 * 10 < 5 > PFU), meanwhile, experimental interference caused by mechanical damage is avoided, the specificity and repeatability of the model are improved, and a higher-quality experimental platform is provided for HSK pathogenesis research and drug screening.

The application discloses a weak strain of Cyprinid herpesvirus 2 (CyHV-2) G-RP7 for preparing a common carp hematopoietic organ necrosis disease vaccine, and the preservation number is CGMCC No. 24099. The weak strain is used to infect a goldfish fin cell line (RyuF-2), and after 4-5 days, typical cytopathic effect (CPE) is observed on the cells, and the continuous passage can stably cause the cytopathic effect. The weak strain is sensitive to the RyuF-2 cell line, realizes in-vitro stable culture and amplification of the weak strain, and the TCID50 of the virus suspension is determined by a Reed-Muench method 50 up to 10 5.9 / ml. The virulence of the weak strain does not return to be strong after continuous in-vitro and in-vivo passages. The weak strain can be used for preparing the common carp hematopoietic organ necrosis disease vaccine, and both immersion immunization and intraperitoneal injection immunization can stimulate the fish to produce specific immune response, and have good anti-CyHV-2 infection effect.

The application discloses an aerosol virus particle detection device, which comprises an aerosol collection module, a microfluidic chip module, a fluorescence detection module and a control system. The microfluidic chip module comprises a first substrate, a detection chamber and a biochip. The other end of the flow guide pipe is communicated with the detection chamber. The biochip comprises a second substrate and a nano array structure. The nano array structure comprises a plurality of first nano columns and a plurality of nano flow guide plates. The plurality of first nano columns and the plurality of nano flow guide plates are alternately arranged in multiple rows. The spacing between adjacent first nano columns and the spacing between adjacent nano flow guide plates in each row gradually decrease along the direction of virus suspension flow. The surface of the first nano column is modified with target virus specific capture antibodies. The application also discloses an aerosol virus particle detection method. The application integrates virus particle transportation, interception and dispersion into one, realizes sufficient combination of virus particles and antibodies, and finally realizes accurate detection of single virus particles through fluorescence labeling.

The invention discloses an adenovirus vector-based 53BP1 mitochondrial directed high expression method and primers, and relates to the technical field of gene engineering. The method comprises the following steps: introducing a mitochondrial positioning signal peptide MTS to an N end of a 53BP1 gene sequence to obtain a 53BP1-MTS fusion gene; carrying out PCR (Polymerase Chain Reaction) amplification on the 53BP1-MTS fusion gene segment; cloning the amplified 53BP1-MTS fusion gene segment into an adenovirus vector to obtain a recombinant adenovirus vector; transfecting the recombinant adenovirus vector into a packaging cell line, culturing and amplifying the virus; and collecting virus suspension, concentrating and purifying, infecting target cells by using the purified adenovirus, and expressing the 53BP1 gene in the target cells. By adopting the method, the directional high-efficiency expression of the 53BP1 in mitochondria can be realized.