4 results about "Virus suspension" patented technology

The invention discloses an organoid gene editing method and application thereof in constructing a disease model. The organ-like gene editing method comprises the following steps: fixing an organ-like; under a microscope, injecting the virus suspension into the organoid; the organoid after injection is cultured; wherein the adopted virus is an adenovirus packaged with an hCas9-P2A-EGFP fusion protein coding sequence driven by a CBh promoter and a gRNA coding sequence driven by two U6 promoters. According to the method, gene editing is directly carried out on a complete organoid based on the CRISPR-Cas9 technology, the organoid gene editing method which is efficient, low in damage and easy and convenient to operate is provided, the related disease model can be rapidly constructed, the occurrence mechanism of human diseases can be simulated, and the method has a good application prospect. Therefore, more stable and reliable technical support and ideal experimental subjects are provided for etiological research, drug research and development, therapeutic schedule verification and the like.

The invention discloses a construction method and system of a herpes simplex keratitis infection model. The method comprises the following steps: fixing a mouse in a mild anesthesia state and enabling eyeballs of the mouse to protrude as much as possible, sucking an HSV-1 virus suspension by using a microsyringe, obliquely inserting a needle from the cornea edge close to the sclera, keeping the inclined surface of the needle upward, slowly penetrating through the cornea epithelium to enter a target position of a matrix layer, and finally obtaining the HSV-1 virus suspension. The needle head is tightly attached to the cornea plane in the injection process; after injection is completed, the needle is slowly withdrawn; in the needle inserting and withdrawing processes, real-time observation is carried out through a stereoscopic microscope to ensure that the needle tip is kept away from the crystalline lens. According to the method, the stable and reliable HSK model can be established under the low virus titer (1 * 10 < 5 > PFU), meanwhile, experimental interference caused by mechanical damage is avoided, the specificity and repeatability of the model are improved, and a higher-quality experimental platform is provided for HSK pathogenesis research and drug screening.

The application discloses a weak strain of Cyprinid herpesvirus 2 (CyHV-2) G-RP7 for preparing a common carp hematopoietic organ necrosis disease vaccine, and the preservation number is CGMCC No. 24099. The weak strain is used to infect a goldfish fin cell line (RyuF-2), and after 4-5 days, typical cytopathic effect (CPE) is observed on the cells, and the continuous passage can stably cause the cytopathic effect. The weak strain is sensitive to the RyuF-2 cell line, realizes in-vitro stable culture and amplification of the weak strain, and the TCID50 of the virus suspension is determined by a Reed-Muench method 50 up to 10 5.9 / ml. The virulence of the weak strain does not return to be strong after continuous in-vitro and in-vivo passages. The weak strain can be used for preparing the common carp hematopoietic organ necrosis disease vaccine, and both immersion immunization and intraperitoneal injection immunization can stimulate the fish to produce specific immune response, and have good anti-CyHV-2 infection effect.

The application discloses an aerosol virus particle detection device, which comprises an aerosol collection module, a microfluidic chip module, a fluorescence detection module and a control system. The microfluidic chip module comprises a first substrate, a detection chamber and a biochip. The other end of the flow guide pipe is communicated with the detection chamber. The biochip comprises a second substrate and a nano array structure. The nano array structure comprises a plurality of first nano columns and a plurality of nano flow guide plates. The plurality of first nano columns and the plurality of nano flow guide plates are alternately arranged in multiple rows. The spacing between adjacent first nano columns and the spacing between adjacent nano flow guide plates in each row gradually decrease along the direction of virus suspension flow. The surface of the first nano column is modified with target virus specific capture antibodies. The application also discloses an aerosol virus particle detection method. The application integrates virus particle transportation, interception and dispersion into one, realizes sufficient combination of virus particles and antibodies, and finally realizes accurate detection of single virus particles through fluorescence labeling.