The application discloses an
aerosol virus particle detection device, which comprises an
aerosol collection module, a
microfluidic chip module, a
fluorescence detection module and a
control system. The
microfluidic chip module comprises a first substrate, a detection chamber and a
biochip. The other end of the flow guide
pipe is communicated with the detection chamber. The
biochip comprises a second substrate and a nano array structure. The nano array structure comprises a plurality of first nano columns and a plurality of nano flow guide plates. The plurality of first nano columns and the plurality of nano flow guide plates are alternately arranged in multiple rows. The spacing between adjacent first nano columns and the spacing between adjacent nano flow guide plates in each row gradually decrease along the direction of
virus suspension flow. The surface of the first nano column is modified with target
virus specific capture antibodies. The application also discloses an
aerosol virus particle detection method. The application integrates virus particle transportation, interception and dispersion into one, realizes sufficient combination of virus particles and antibodies, and finally realizes accurate detection of single virus particles through
fluorescence labeling.