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Univalent and bivalent inactivated vaccine for hand-foot-and-mouth disease and preparation method thereof

A technology for inactivated vaccines and hand, foot and mouth disease, applied in botany equipment and methods, biochemical equipment and methods, microorganism-based methods, etc., can solve the problem of no prevention

Inactive Publication Date: 2010-04-21
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no inactivated vaccine for hand, foot and mouth disease to prevent EV71 and Cox.A16 viruses in my country

Method used

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  • Univalent and bivalent inactivated vaccine for hand-foot-and-mouth disease and preparation method thereof
  • Univalent and bivalent inactivated vaccine for hand-foot-and-mouth disease and preparation method thereof
  • Univalent and bivalent inactivated vaccine for hand-foot-and-mouth disease and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Embodiment 1: Preparation of hand, foot and mouth disease inactivated vaccine of the present invention

[0050] 1. Detection of enterovirus EV71 and Cox.A16 strains

[0051] (1) Viral titration determination

[0052] The Reed&Muench method is adopted to measure, and the highest virus dilution factor that can cause more than 50% of Vero cell lines to present the typical enterovirus cytopathic phenomenon is taken as the virus potency value (TCID 50 ):

[0053] Using the 199 medium without fetal bovine serum as the diluent, make ten-fold serial dilutions of the tested virus strains, and obtain different dilution ratios (1-10 10 ) virus strains were inoculated with monolayer Vero cell strains (2×10 4 cells / well) 8 replicate wells, and inoculate the same volume of 199 medium as negative control. In 199 medium containing 2% fetal bovine serum, 5% CO 2 and cultured at 37°C. After culturing, observe the results of the typical enterovirus cytopathic phenomenon of the Vero ...

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Abstract

The invention discloses a univalent and bivalent inactivated vaccine for preventing Enterovirus 71 (EV71) and Coxsackie virus A16 (Cox.A16) of hand-foot-and-mouth disease, and a preparation method thereof. The preparation method comprises the following steps: screening vaccine strains for the hand-foot-and-mouth disease; establishing and verifying a vaccine strain third-stage seed lot library; culturing cells; inoculating and propagating virus; collecting virus suspension; inactivating the virus; ultra-filtering, concentrating and purifying the virus suspension to obtain vaccine stock solution; and finally preparing the univalent and bivalent inactivated vaccine. The univalent and bivalent inactivated vaccine has good application prospect for preventing the hand-foot-and-mouth disease.

Description

technical field [0001] The invention belongs to the field of biopharmaceuticals, and relates to a vaccine and a preparation method thereof, in particular to a hand-foot-mouth disease single- and double-valent inactivated vaccine and a preparation method thereof. Background technique [0002] Hand, foot and mouth disease is caused by viruses of the family Picornaviridae and the genus Enterovirus, and has become an important infectious disease that endangers the health of infants and young children and public health safety. HFMD is caused by enteroviruses such as Coxsackieviruses A4, A5, A9, A10, A16, B2, B5 and Enterovirus 71, but mainly by Enterovirus 71 (EV71) and Coxsackieviruses Caused by virus type 16 (Cox.A16). The most common feature of the disease is a rash on the buccal mucous membranes, which soon appears on the hands and feet. Clinical symptoms caused by infection with EV71 and Cox.A16, such as hand-foot-mouth disease and herpetic angina, are difficult to disting...

Claims

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Application Information

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IPC IPC(8): A61K39/125A61K39/295A61P31/14C12N15/866C12N7/04C12R1/93
Inventor 赵忠鹏李敏高啸罗德炎刘鑫李晓楠邢丽段越强杨鹏辉王希良
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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