575results about How to "No cross reaction" patented technology

Belonging to the field of biotechnologies, the invention discloses a competitive ELISA kit for detection of a peste-des-petits-ruminants virus antibody. The kit comprises a detection system composed of a coating antigen reaction solution and a monoclonal antibody reaction solution. The kit adopts prokaryotically expressed peste-des-petits-ruminants Nigeria 75 / 1 strain N protein as the coating antigen and employs a monoclonal antibody against N protein as the competitive antibody. The antibody against a peste-des-petits-ruminants virus in sheep serum is detected according to a competitive ELISA principle. The kit provided in the invention can rapidly and specifically detect the peste-des-petits-ruminants virus antibody in serum, and simultaneously has the advantages of large-scale production of monoclonal antibodies, good reaction specificity, high sensitivity, simple operation, low cost, stable, reliable and easily observable reaction results, thus being very suitable for import and export quarantine of sheep, food hygiene and screening of large batches of samples in livestock breeding farms, and being easy for large-scale popularization and application.

The invention belongs to the technical field of nucleic acid detection, and relates to a mycobacterium tuberculosis complex detection kit and method based on a CRISPR-Cas12a system. According to the kit, the detection sensitivity is improved by adopting a recombinase polymerase amplification technology, CRISPR-Cas12a is used for specifically targeting a mycobacterium tuberculosis complex target sequence and then activating the bypass cutting activity of Cas12a, and a mycobacterium tuberculosis complex can be sensitively and specifically detected from sputum. The kit and method have the advantages of being non-invasive, capable of detecting the mycobacterium tuberculosis complex frequently and repeatedly, high in detection speed and the like. Compared with an existing PCR detection method,the method based on the CRISPR-Cas12a system does not need a thermal cycler with heating and cooling functions, can detect the mycobacterium tuberculosis complex in sputum through fluorescence reading, has the advantages of being low in cost, easy and convenient to operate, high in sensitivity, good in specificity and the like and is suitable for clinical large-scale application.

The invention provides an immunity diagnosis test kit for detecting II-type dengue virus antigen, which comprises a porous reaction plate covering monoclonal antibody DV2-M6, a sample treatment liquid, a monoclonal antibody DV2-M15 marked with a label, a positive contrast, a negative contrast, a concentration washing liquid, a develop liquid and a termination liquid, wherein the monoclonal antibodies DV2-M6 and DV2-M14 of the test kit can be specifically combined with NS1 protein of II-type dengue virus, without cross reaction with other three kinds of serotype dengue viruses NS1 and respectively combined with different antigen points of NS1, while the check sensitivity of NS1 protein of II-type dengue virus can reach 3ng / ml and the check sensitivity of culture supernatant of II-type dengue virus infection cell is 8 power of Pan-E dengue early elisa test kit, thereby improving the sensitivity of clinical serum sample check.

The invention provides a set of nucleic acids of a liquid-phase gene chip for synchronously detecting five porcine viruses, which comprise forward and reverse primers and hybrid probes for porcine reproductive and respiratory syndrome virus (PRRSV), porcine circovirus type 2 (PCV2), porcine pseudorabies virus (PRV), classical swine fever virus (CSFV) and porcine parvovirus (PPV). The invention also provides a multiplex liquid-phase chip high-flux molecular biology detection method of the five porcine viruses. According to the method, porcine virus nucleic acids in the sample to be detected are extracted to perform multiplex unsymmetric nucleic acid amplification / multiplex liquid-phase gene chip (suspension chip) combined detection, thereby synchronously and accurately detecting and identifying the five porcine viruses in the sample to be detected. The method has the advantages of high specificity, high sensitivity, high stability, high flux and high detection speed, and is simple to operate.

The invention discloses a fluorescent microsphere lateral chromatographic detection strip for multiple joint inspection of trace target substances as well as a preparation method and application thereof. The fluorescent microsphere lateral chromatographic detection strip is characterized by comprising a soleplate, wherein the top of the soleplate is sequentially provided with a sample absorption pad, a combination pad, a chromatographic film and a piece of water absorption paper from left end to right end, the right end of the sample absorption pad is pressed to be attached onto the left end of the combination pad, the right end of the combination pad is pressed to be attached onto the left end of the chromatographic film, the left end of the water absorption paper is pressed to be attached onto the right end of the chromatographic film to form a lateral chromatographic detection structure; the chromatographic film is provided with a detection line and a quality control line from left to right; the combination pad is formed by a polyester fiber film and fluorescent microspheres marked with different ligands on the polyester fiber film, and the chromatographic film consists of a nitrocellulose film and a plurality of detection lines which are arranged on the nitrocellulose film and contain different ligands which can be specifically combined with the target substances. The fluorescent microsphere lateral chromatographic detection strip can be used for performing multiple joint inspection on a plurality of trace target substances simultaneously, all display results have no cross reaction and are dependent from one another, the detection sensitivity and accuracy can be remarkably improved, rapidness and convenience in operation can be realized, and an important significance on biological detection and early diagnosis and treatment of diseases can be achieved.

The invention relates to a primer, probe and reagent kit for identifying Brucella S2 vaccine strains in aerocolloid. The forward primer sequence of Brucella-RPA-LFD is shown as SEQIDNo.1, the backward primer sequence of Brucella-RPA-LFD is shown as SEQIDNo.2, and the probe sequence of Brucella-RPA-LFD is shown as SEQIDNo.3; the forward primer sequence of S2-RPA-LFD is shown as SEQIDNo.4, the backward primer sequence of S2-RPA-LFD is shown as SEQIDNo.5, and the probe sequence of S2-RPA-LFD is shown as SEQIDNo.6. The combination of the RPA-LFD primer and the probe, or the reagent kit, for identifying and diagnosing the Brucella S2 vaccine strains, provided by the invention are high in sensitivity and specificity, and can at least detect 2.5*100 cfu of Brucella or the Brucella S2 vaccine strains.

This invention relates to a method of detection of food-borne pathogenic bacteria using multiple fluorescent PCR and modified molecular beacons. Several pairs of primers and modified molecular beacon probes are designed according to the specific gene sequence of the pathogenic bacteria to be tested, which is amplified using fluorescent PCR and modified molecular beacon. The amplified product is crossbred with molecular beacon, and the fluorescent intensity of the reaction system is tested with the necessary circle times for intended threshold as a judgment for results, that is, negative for Ct of 0 or 40 and positive for Ct less than 38. This invention, which is advanced in high sensibility, significant specificity, simple operation and intuitionistic observation, is applicable in simultaneous detection of any two kinds, three kinds and four kinds of randomly combined bacteria and food-borne pathogenic bacteria as well as specimen in large amounts.

The invention discloses a spray for killing mites on the body surface of a pet and a preparation method of the spray. The spray is simple in preparation method, convenient to use, good in curative effect and high in safety. The spray for killing the mites on the body surface of the pet is technically characterized by comprising the following ingredients in parts by weight: 75-95 parts of alcohol, 0.1-1 part of flucycloxuron, 0.1-1 part of phoxim, 5-20 parts of traditional Chinese medicine combination extracts, and 0.1-1 part of eugenol, wherein a traditional Chinese medicine composition comprises the following raw materials in parts by weight: 15-20 parts of radix stemonae, 3-5 parts of fructus cnidii, 3-5 parts of sophora flavescens, 2-4 parts of cortex phellodendri, 3-5 parts of cortex dictamni, and 4-7 parts of fructus kochiae. The spray belongs to the technical field of animal medicines.

The invention provides a test kit for detecting nucleic acid of respiratory tract pathogens. The kit comprises a CRISPR-Cas detection system: crRNA, a primer pair, Cas protein and a nucleic acid probethat are described in the invention. The invention further provides a detection method of the nucleic acid of the respiratory tract pathogens, a combination of the crRNA and the primer pair and application of the combination. The test kit and the detection method disclosed by the invention can be used for directly observing results by naked eyes without depending on a large instrument, and detection can be realized under a mild condition, so that the detection is more convenient; the test kit and the detection method provided by the invention can be used for efficiently and rapidly detecting / diagnosing the respiratory tract pathogens (such as flu A, flu B and COVID-19), have relatively high specificity and sensitivity, and can be used for detecting and screening the respiratory tract pathogens, for example, rapidly distinguishing various respiratory tract pathogens including the flu A, the flu B and the COVID-19.

The invention relates to a double-antibody biotin-Avidin ELISA (enzyme-linked immuno sorbent assay) detection kit for cattle viral diarrhea virus antigen and an application method thereof. The kit comprises washing liquid for ELISA detection, developing liquid, stopping liquid, Streptavidin-HRP, positive control and negative control and is characterized by further comprising an ELISA plate coating cattle viral diarrhea virus single antibody 3D8 and a cattle viral diarrhea virus single antibody 3F9 marked by biotin. The detection without cross reaction is performed by using the kit, thereby being capable of preventing leak detection caused by low titer in the antibody and having high specificity; a biotin-affine sensitizer is used for amplification, thereby increasing the flexibility, improving the detection rate, and simultaneously reducing the amount of second antibody; and the kit is suitable for being used widely.

The invention relates to an African swine fever fluorescent PCR (polymerase chain reaction) assay reagent, an African swine fever fluorescent PCR assay kit and application thereof, which belong to the field of bioengineering. With the p72 gene as a reference, a pair of specific PCR primers and a TaqMan fluorescent probe are designed and optimized. Moreover, the primers are designed with the full length of the p72 gene to amplify the p72 gene, and the PCR product is cloned into a pGEM-T vector. Standard curves are drawn with a positive plasmid as a standard product, and the assay range is 10 to 1.0*10<7> copies. In the whole process of assaying African swine fever by an African swine fever fluorescent PCR method disclosed by the invention, only 2.5 to 3 hours are taken from DNA extraction to the appearance of an assay result, manual operation is greatly reduced, and time is greatly shortened. Furthermore, a plurality of samples can be assayed each time, and particularly assay of a large batch of samples can be realized.

The object of the present invention is to provide a diagnostic method by which Helicobacter pylori infection can be diagnosed at low cost without causing pain on subjects and without requiring particular equipment and which is free of cross reactivity and excellent in specificity without variation among lots as a result of the use of a single antibody facilitating the quality control and which shows good sensitivity even when a single monoclonal antibody is used.The present invention provides a monoclonal antibody which recognizes Helicobacter pylori catalase as an antigen.

The invention discloses a combination of primer and probe for rapidly detecting pasteurella multocida on site by RPA-LFD. A forward primer sequence is shown in SEQ ID No. 1; a reverse primer sequence is shown in SEQ ID No. 2; a probe sequence is shown in SEQ ID No. 3. The invention also discloses a kit for detecting pasteurella multocida. The pasteurella multocida RPA-nfo detection combination of primer and probe and kit have high sensitivity and strong specificity, at least can detect six copied / reacted Pasteurella multocida DNAs, and can perform sensitive, specific and rapid detection of Pasteurella multocida DNA on crude lysate of a sample to be detected within 25 min by means of a constant-temperature water bath kettle or human armpit temperature without special instrument and equipment, so as to be suitable for the diagnosis of the pasteurella mutocida disease on site or base.

The invention relates to a newcastle disease virus / avian influenza virus H9 subtype / infectious bronchitis virus triplex fluorescence quantification detection reagent and a detection method and belongs to the technical field of animal quarantine. A newcastle disease virus M gene coding region specific sequence, an avian influenza virus H9 subtype H gene coding region specific sequence and a chicken infectious bronchitis virus M gene coding region specific sequence are selected as target regions, and on the basis of multi-sequence comparison, primer and probe design is conducted. The length of primers is about 20 basic groups, the GC content is 50-60%, a two-stage structure and repeatability do not exist in the primers, no complementary sequence exists between the primers or in the primers, and the melting temperature (Tm value) difference between the primers is smaller than 5 DEG C. In order to guarantee universal use of a newcastle disease virus probe, the length of the probe is only 13 basic groups, the probe is modified by LAN, and the Tm value of the probe is increased. The lengths of the other two virus probes are both about 25 basic groups, and the Tm values are about 5 DEG C higher than those of the primers.

The invention refers to a kind of Clenbuterol Hydrochloride semiquantitative quick measuring method. The invention pastes solution sample absorbing part, colloidal gold label part, measurement reaction part and water absorbing part on the back lining of the reagent paper. The measurement part is enclosed with 1-3 stripes of Clenbuterol Hydrochloride antibodies or 1-3 stripes of measuring antigens which are sued as measuring lines, at the same time, it is enclosed with 1-3 stripes of the second species animal protein resisting IgG which are used as reference lines. When combining, the measuring lines and the reference lines couldn't more than 1 stripe at the same time, the compound lines are 2-4 stripes. The quick measuring reagent paper has a strong specificity, the sensitivity can reach 0.09ng.mL, it can be used under temperature of 4-40 degrees, the result can be observed after two minutes.

The invention relates to a dihydrocapsaicin artificial hapten and artificial antigen and as well as preparation methods thereof. The molecular structural formula of the dihydrocapsaicin artificial hapten is shown in the formula I; the molecular structural formula of the artificial antigen is shown in the formula II. According to the invention, the phenolic hydroxyl group of N-(4-hydroxy-3-methoxyl benzyl) is modified; the dihydrocapsaicin artificial hapten retains characteristics and structure of dihydrocapsaicin matters to the maximum extent, can be used as antigenic determinant, and has reactive groups which can be coupled with carrier protein; the obtained specific dihydrocapsaicin artificial antigen can acquire a dihydrocapsaicin antibody which is high in appetency, sensitivity and specificity in an immunizing manner, and can be used for immunity detection for dihydrocapsaicin in edible vegetable oil.

The invention discloses preparation of novel duck reovirus recombinant sigma B protein antigen, and application of the novel duck reovirus recombinant sigma B protein antigen in detection of a novel duck reovirus antibody. With RNA (ribonucleic acid) of the novel duck reovirus (NDRV) as a template, a nucleotide complete sequence of an NDRV S3 gene coding region can be amplified through a reverse-translation polymerase-chain reaction, is directionally subcloned to a fusion expression vector of the prokaryotic expression vector pET-30a-C(+) of escherichia coli, and transformed into the host cell of the Escherichia coli BL21 (DE3), and is subjected to inducible expression with IPTG (isopropyl-beta-d-thiogalactoside), purification of Ni-NTA Agarose, inclusion body refolding to obtain the expressed NDRV alpha B recombinant protein antigen. The expression mode of the protein is fusion protein (His-alpha B) with molecular weight being about 44kU; and immunoblotting assays prove that the protein has immunoreactivity similar to natural protein..

The invention, which belongs to the technical fields of immunology and food safety analysis, relates to an enzyme-linked immunosorbent assay kit for detecting skeletal muscle troponin I of cattle or sheep, and a preparation method thereof and application thereof. The kit comprises an enzyme label plate coated with a capture antibody, a horseradish-peroxidase-labeled detection antibody, a skeletalmuscle troponin I standard solution of cattle or sheep, a substrate coloured solution, a stop solution, and a concentrated washing solution. The capture antibody is obtained by secretion of a hybridoma cell line 3A8 with the preservation number of CCTCC NO:C2018217; and the detection antibody is obtained by secretion of a hybridoma cell line 3D3 with the preservation number of CCTCC NO:C2018218. The kit has advantages of high sensitivity, high precision, high accuracy, and low cross-reaction rate and is suitable for large-scale sample detection.

The invention provides a double FQ-PCR detection kit for identifying PCV (porcine circoviruses) type 2 and type 3. By aiming at the characteristic of low Rep protein and Cap protein gene homology of PCV2 and PCV3, a pair of specific primers and a TaqMan MGB probe (SEQ ID NO:1-6) are designed by using Rep protein gene of PCV2 and Cap protein gene of PCV3 as amplification target regions; the real-time fluorescence quantitative PCR technology is used; the identification detection of the PCV2 and the PCV3 is realized. The detection kit provided by the invention is applicable to viral nucleic aciddetection in samples such as nose swab, oral cavity secreta, faeces, serum, lungs, lymph gland and kidneys of a suspected PCV infectious pig; the sensitivity can reach 1.0*10<1> copy / mu L; the crossedreaction with other pathogene capable of generating mixed infection or with similar of infection symptoms do not exist.

The invention discloses a universal primer and probe combination for detecting foot and mouth disease viruses through RPA (recombinase polymerase amplification)-lateral flow assay technology. The forward primer sequence is shown as SEQ ID No.1, the reverse primer sequence is shown as SEQ ID No.2, and the probe sequence is shown as SEQ ID No.3. The invention also discloses a kit for detecting foot and mouth disease viruses. Detection with the primers and probes involved in the invention only needs virus RNA extraction on clinical samples and isothermal amplification after one-step process reverse transcription into cDNA, thermal cycling reaction is not needed, amplification in a PCR instrument is unnecessary, and the result can be clearly displayed on a lateral flow chromatography test strip. The primer and probe combination and the kit provided by the invention have the advantages of high sensitivity, strong specificity, simple reaction procedure, short detection time and the like, and are suitable for rapid, accurate and simple detection of foot and mouth disease viruses on a cattle farm.

An anti-beta-interferon monoclonal antibody is generated by a hybridoma cell strain having a preservation number of CCTCC NO:C201121. A monoclonal antibody specifically combined with the beta-interferon having a sequence represented by SEQ ID No1 has a neutrality effect on the beta-interferon. The anti-beta-interferon monoclonal antibody can be used for the affinity chromatography purification and ELISA detection of the beta-interferon.

The invention relates to the technical field of detection of pathogenic microorganisms, in particular to a detection marker, primer probe pair, kit and detection method for herpesvirus hominis I typeand II type. By taking reverse repeated sequences RS1 and RS2 in an HSV genome as a target area, the detecting sensitivity can be further improved as two copies are available in the genome on the onehand and cross reactions are unlikely to be caused as the inter-nucleic acid similarity is low on the other hand.

The invention relates to a primer and a method for detecting GI type and GII type norovirus by utilizing the primer, belonging to the technical field of biological detection. The sequence of the primer is as follows: Nov-F: 5-GTGAATGAWGATGGCGTCKA-3, Nov-R: 5-GTHAGWATCCAGGGGTCAAT-3. The invention focuses on the design of the sequence of the primer, the composition of an RT-PCR (reverse transcription-polymerase chain reaction) system, the selection of reaction conditions and the judgment of reaction results can be performed according to the conventional methods in the field. By adopting the primer and the method provided by the invention, a pair of primers can simultaneously detect and differentiate the GI type and the GII type norovirus in a same reaction tube, thereby not only simplifying the operation steps, but also shortening the detection time.

The invention discloses a preparation method of immunogold rapid test paper for vibrio parahaemolyticus. The method comprises the following steps of: (a) preparing a vibrio parahaemolyticus antigen; (b) preparing a vibrio parahaemolyticus inactivated vaccine; (c) preparing a polyvalent antiserum with a vaccine immune experimental rabbit; (d) purifying the antiserum; (e) marking with colloidal gold; and (f) preparing an immunogold test strip. A detection technology in which the immunogold rapid test paper for vibrio parahaemolyticus is adopted has the advantages of easiness, sensitivity, rapidness, specificity and the like; the customs clearance speed of port imported and exported goods can be increased greatly; and meanwhile, the test paper has the advantages of low price, no need of instrument, easiness and rapidness for operationing and easiness in judging results.

The invention relates to a hybridoma cell strain 1C8 and an anti-aflatoxin G1 monoclonal antibody produced by the same. The hybridoma cell strain 1C8 (CCTCC NO: C201017) provided by the invention can be used for preparing a high titer anti-aflatoxin G1 monoclonal antibody. The titer of an anti-aflatoxin G1 mouse ascites antibody measured by enzyme-linked immunosorbent assay (ELISA) can reach 8.19*10<5>. The anti-aflatoxin G1 monoclonal antibody provided by the invention has high sensitivity and good specificity, has a 50% inhibition concentration IC50 for aflatoxin G1 being 13.92 ng / mL, has no cross reaction with aflatoxin B1, aflatoxin B2, or aflatoxin M1, and can be used for the determination of G1 aflatoxin.

The invention discloses a primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through a RPA-lateral flow assay technology. A primer probe liquid, popular primer and probes are arranged in the kit, wherein the primer probe liquid consists of a popular subtype primer which is used for detecting the foot-and-mouth disease virus through an RPA technology, and the probes, and the popular primer and the probes are in the primer probe liquid. The primer, the probe and the kit, which are disclosed by the invention, adopt the RPA-Lateral flow assay technology to establish a method for rapidly detecting the FMDV of cattle for the first time, and through the evaluation of specificity and evaluation of sensitivity, the sensitive, reliable and new method can be used for a clinical detection on site of the FMDV. Through the combination of the primer and the probes, which are disclosed by the invention, the method for detecting the FMDV in the aerosol in a subtype manner through the RPA technology has the advantages of being higher in sensitivity, specificity and repeatability.

The invention discloses a rapid quantitative detection card for a canine distemper virus antibody and a using method. The rapid quantitative detection card comprises a detection card shell and a teststrip assembled in the detection card shell. The test strip comprising a plastic base plate with pressure-sensitive adhesive. A sample pad, a marker pad, a nitrocellulose membrane and absorbent paperare sequentially pasted on the base plate. The marker pad is composed of a carrier base layer and a marker, wherein the marker is a membrane formed by spraying the carrier base layer with lanthanide fluorescent detection microspheres and lanthanide fluorescent quality control microspheres. The part, coated with a canine distemper virus H protein antigen, of the nitrocellulose membrane is a detection line. The part, coated with an anti-Chicken IgY antibody, of the nitrocellulose membrane is a quality control line. The marker is fluorescent detection microspheres marked with a canine distemper virus structural protein H protein recombinant antigen and fluorescent quality control microspheres marked with the anti-Chicken IgY antibody. By the adoption of the rapid quantitative detection card,on-site rapid quantitative determination of the canine distemper virus antibody can be achieved, and the practical value and the promotional value are higher.

The invention discloses a duplex fluorescent RT-LAMP detection group and kit for visually identifying foot-and-mouth disease virus and Bluetongue virus and an application of the detection group and the kit. The duplex fluorescent RT-LAMP detection group comprises two groups of specific primers and probes, the first group of specific primers and probes comprise FMDV-F3, FMDV-B3, FMDV-FIB(F1c-F2), FMDV-BIP(B1c-B2) and FMDV-probes, the second group of specific primers and probes comprise BTV-F3, BTV-B3, BTV-FIB(F1c-F2), BTV-BIP(B1c-B2) and BTV-probes, and the two groups of specific primers and probes are as shown in a sequence table SEQ ID No.1 to SEQ ID No.10. The duplex fluorescent RT-LAMP detection group can identify and diagnose the foot-and-mouth disease virus and the Bluetongue virus inthe same reaction tube and has the advantages of good specificity, high sensibility, less pollution, convenience, rapidness and the like, a detection result can be directly observed by naked eyes andis judged according to colors of reaction products, and the detection group can be used for basic-level quarantine with poor conditions.

The invention discloses an LAMP primer group and an LAMP detection kit for staphylococcus aureus and a use method of the detection kit. The LAMP primer group for staphylococcus aureus comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. According to the LAMP primer group, the LAMP detection kit and the use method, loop-mediated isothermal amplification primers are designed according to the sequences of the conservative regions of the heat-resistant nuclease genes (nuc) of staphylococcus aureus, the specific regions of target genes are amplified by adopting the LAMP technology, thus the rapid detection for staphylococcus aureus is realized from the molecular level, and an effective and rapid nucleic acid screening detection method is provided for detecting staphylococcus aureus.