The invention discloses a primer, probe and kit for detecting foot-and-mouth disease virus in aerosol through a RPA-lateral flow assay technology. A primer probe liquid, popular primer and probes are arranged in the kit, wherein the primer probe liquid consists of a popular subtype primer which is used for detecting the foot-and-mouth disease virus through an RPA technology, and the probes, and the popular primer and the probes are in the primer probe liquid. The primer, the probe and the kit, which are disclosed by the invention, adopt the RPA-Lateral flow assay technology to establish a method for rapidly detecting the FMDV of cattle for the first time, and through the evaluation of specificity and evaluation of sensitivity, the sensitive, reliable and new method can be used for a clinical detection on site of the FMDV. Through the combination of the primer and the probes, which are disclosed by the invention, the method for detecting the FMDV in the aerosol in a subtype manner through the RPA technology has the advantages of being higher in sensitivity, specificity and repeatability.