132 results about "Pasteurella" patented technology

The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure with a targeted size that are substantially monodisperse in size. The present invention also relates to methodology for polymer grafting by a polysaccharide synthase to form glycosaminoglycan polymers having an unnatural structure.

The invention discloses a Chinese medicinal herb additive for meat chickens and application. The Chinese medicinal herb additive comprises heterophylly falsestarwort root, nutgrass galingale rhizome, amur corktree bark, coptis root, medicated leaven, sanguisorba, hawthorn, Indian Buead, officinal magnolia bark, largehead atractylodes rhizome, tangerine peel, malt, indigowoad root, swordlike atractylodes rhizome, indigowoad leaf, cyrtomium fortune, chatoyancy and calamus; and mixture of the compositions is added with calcium bicarbonate, phytase, mulitivitamin for birds and lysine. The Chinese medicinal herb additive effectively promotes absorption of nutrient, can resist bacteria and virus, strengthen immunity of the meat chickens, effectively prevents bird flu, has obvious curative effect on diseases having respiratory tract symptoms as the major symptom, and has inhibiting function on familiar newcastle disease, colon bacillus, Pasteurella, enteritis, diarrhea, feather pecking, hair shedding, anus pecking, coccidiosis and the like. The additive cannot restrict the growth and disease resistance of the meat chickens, the meat quality of the meat chickens fed by the additive is obviously better than that of the meat chickens fed by the prior feed additive, the average daily gain of the meat chickens is improved by 23 to 28 percent than that of the meat chickens fed by the prior feed additive, the cultivation cycle is obviously shortened, and no harmful substance is deposited.

The present invention is concerned with bacteria for the production of succinic acid. Specifically, the invention relates to a bacterial cell of the genus Pasteurella comprising a heterologous polypeptide having isocitrate lyase activity and a heterologous polypeptide having malate synthase activity. Further, the present invention contemplates a polynucleotide comprising a nucleic acid encoding a polypeptide having isocitrate lyase activity and a nucleic acid encoding a polypeptide having malate synthase activity. Finally, the present invention relates to the use of a bacterial cell of the invention for the manufacture of succinic acid.

The invention relates to an application of pink plumepoppy herb extract in veterinary drugs of economic animals; the pink plumepoppy herb extract is used for preparing medical feed additives of the veterinary drugs which are used for enhancing the growth of the economic animals and increasing conversion rate of the feed or veterinary drugs which has better inhibiting action and disinfecting action to common pathogenic bacteria in livestock and poultry, such as listeria monocytogenes, haemophilus and pasteurella.

The invention discloses a method for preparing an actinobacillus pleuropneumoniae (App) bacterial ghost and a method for preparing a subunit vaccine by loading a pasteurella antigen with the App bacterial ghost. A recombinant swine App bacterial ghost is prepared by controllable double-cracking technology and a pasteurella protection gene is introduced into an App bacterial ghost carrier, so that swine pleuropneumonia and a pasteurella bigeminal gene vaccine for preventing and treating swine pasteurellosis and swine pleuropneumonia are obtained. The preparation of the bacterial ghost carrier and the application of the bacterial ghost carrier to the prevention and treatment of important animal epidemic diseases are realized and a method is provided for the research of a multi-geminal gene vaccine at the same time. An animal experiment indicates that the protection rates of the bigeminal vaccine on infectious swine pleuropneumonia and pasteurellosis are up to 99 percent and 99.2 percent respectively.

The present invention relates to methodology for polymer grafting by a polysaccharide synthase and, more particularly, polymer grafting using the hyaluronate or chondroitin or heparin/heparosan synthases from Pasteurella, in order to create a variety of glycosaminoglycan oligosaccharides having a natural or chimeric or hybrid sugar structure with a targeted size that are substantially monodisperse in size. The present invention also relates to methodology for polymer grafting by a polysachharide synthase to form glycosaminoglycan polymers having an unnatural structure.

The invention relates to the field of biologically-derived insecticidal preparations, in particular to a complex microbial inoculant for controlling root-knot nematodes of crops and a preparation method of the complex microbial inoculant. The complex microbial inoculant for controlling the root-knot nematodes of the crops and the preparation method of the complex microbial inoculant are characterized in that microorganism is composed of bacillus subtilis, bacillus sphaericus, pseudomonas fluorescens, bacillus thuringiensis, brevibacillus laterosporus, paenibacillus polymyxa, pasteurella pneumoniae, paecilomyces lilacinus, verticillium chalamydosporium, beauveria bassiana and aspergillus fumigatus. The novel multi-bacteria same-pot fermentation technology is adopted, the formula is clever and reasonable, the disadvantages of mutual antagonism in the liquid deep fermentation culture of compound bacteria are eliminated, the compound bacteria has high activity, and the complex microbial inoculant for controlling the root-knot nematodes of the crops has a good control effect on tomatoes and cucumber root-knot nematodes.

The invention belongs to the technical field of the research and development of medical products, and relates to the separation and identification of syringa amurensis rupr. bark volatile oil and a novel application. In the invention, syringa amurensis rupr. barks are taken, cut up after being dried, and processed by ultrasonic for one hour after being soaked by using distilled water for 6 to 8 hours, the temperature is 30 DEG C, and the power is 80W; medicinal materials and soaking liquid are poured into a volatile oil extractor together and are extracted for 4 to 5 hours by adopting a steam distillation method; a water layer is discharged, and the volatile oil extractor is repeatedly washed by ether to enable syringa amurensis rupr. volatile oil to be fully dissolved into the ether; and ether solution dissolved with the volatile oil is poured into a beaker, then anhydrous sodium sulfate is added into the beaker to suck up water, pure volatile oil is obtained after the ether is recovered, and the pure volatile oil is put into a refrigerator of 4 DEG C and preserved for standby. The syringa amurensis rupr. bark volatile oil is analyzed by using a 6890/5973N gas phase color spectrum and mass spectrum united instrument, and comprises the main chemical components of 27.46% of n-hexadecanoic acid, 28.52% of 9,12-octadecadienoic acid (Z,Z), 14.16% of 9,17-octadecadienal and 2.44% of tricosane. The syringa amurensis rupr. bark volatile oil has a suppressing function with different degrees for colibacillus, staphyiococcus aureus, pasteurella, streptococci and salmonella, can be developed into a natural antimicrobial agent applied to the industries of foods, medicines and the like, and can prepare various forms of products with a bacteriostatic function.

The invention discloses bovine capsular serotype A Pasteurella mutocida and application thereof. The bovine capsular serotype A Pasteurella mutocida is separated from disease samples with haemorrhagic septicomia of cattle in six provinces (cities) with the microbial preservation number of CGMCC No.3619. The bovine capsular serotype A Pasteurella mutocida is cultured by BHI, oil adjuvant is adopted to prepare inactivated immunogen, and a mouse model proves that the bovine capsular serotype A Pasteurella mutocida has complete protecting function on serotype A Pm velogenic attck, and does not have crossing protection with serotype B Pm. Testing results prove that vaccine can be prepared by the bovine capsular serotype A Pasteurella mutocida for preventing the haemorrhagic septicomia of cattle caused by the capsular serotype A Pasteurella mutocida. The strain cannot cause diseases when inoculated to chicks, which proves that the strain is chicken isolated capsular serotype A Pasteurella mutocida.

The invention belongs to the field of veterinary medicines, and in particular relates to traditional Chinese medicine oral liquid for treating calf diarrhea and a preparation method of the traditional Chinese medicine oral liquid. The traditional Chinese medicine oral liquid is prepared from 19-20 parts of golden threads, 20-30 parts of Chinese pulsatilla roots, 27-29 parts of honeysuckle flowers, 30-32 parts of scutellaria baicalensis, 30-31 parts of licorice roots, 25-30 parts of sweet wormwood herbs and 27-30 parts of oriental wormwoods. A Chinese herbal compound can be prepared into yellow and white honeysuckle flower oral liquid, so that the functions of dispelling dampness, cooling down a fever, resisting bacteria and eliminating inflammation, effectively inhibiting pathogenic bacteria such as escherichia coli, salmonella and pasteurella can be achieved while the spleen and stomach can be protected and the immunity of the organism is increased; under the combination of prevention and control, the calf diarrhea can be effectively treated. A clinical test proves that by using the traditional Chinese medicine oral liquid, the cure rate of the calf diarrhea reaches 86.7 percent; in addition, the medicinal raw materials of traditional Chinese medicine oral liquid are wide in sources and low in cost; as a whole, the cost of the traditional Chinese medicine composition is reduced.

The invention relates to a haemophilus parasuis detection kit and a detection method thereof, and belongs to the technical field of molecular biology. The detection kit comprises a primer pair, PCR Mix, a position control and dd H2O. The haemophilus parasuis detection kit disclosed by the invention has the primer pair designed according to an mviN gene sequence in a high conserved domain, is good in specificity, and can accurately distinguish the haemophilus parasuis strain LC from haemophilus paragallinarum, actinobacillus pleuropneumoniae, pasteurella muhocida, arcanobacterium pyogenes, staphylococcus aureus and streptococcus suis; the detection kit and the detection method provided by the invention are high in sensitivity, short in consumed time, accurate in detection, and important in significance of monitoring haemophilus parasuis reproduction, disease occurrence and prevalence as well as timely control of the disease.

The invention discloses compound microbial fertilizer and a preparation method thereof and belongs to the technical field of agricultural bio-fertilizer. The fertilizer comprises dried chicken manure, rice chaff, cane sugar, urea, calcium superphosphate, potassium sulphate, zeolite powder, ATCC144712 azotobacter chroococcum, CICC20026 rhizobium astragali, ATCC6542 bacillus pasteurella, ATTC842 paenibacillus polymyxa, ACCC10168 bacillus megatherium, ATTC527 saccharomycetes and ATTC161 filamentous bacteria. According to the compound microbial fertilizer, through innovative technologies such as screening and matching, solid fermentation, negative pressure low temperature drying, two-section pelleting and particle high polymer material peridium for microbial bacteria, the novel biological environment-friendly type fertilizer integrating organic constituents and inorganic constituents is developed out, and the fertilizer has the advantages that the fertilizer consumption is reduced, the fertilizer utilization rate is raised, the agricultural product quality is improved, the environment is protected, and the fertilizer serves as a production means of green foodstuff.

The invention discloses a haemophilus parasuis outer membrane protein P5 (OMP5) resistant monoclonal antibody, a hybridoma cell strain and an application. The hybridoma cell strain is preserved in the China center for type culture collection (CCTCC), and the preservation serial number is CCTCCC2012135. The monoclonal antibody prepared by the hybridoma cell strain is good in specificity, high in valence, high in generality, free from cross reaction with swine Escherichia coli, swine pasteurella, swine pleuropneumonia actinobacillus, streptococcus suis and swine bordetella bacilli, capable of detecting haemophilus parasuis with different serotypes and widely applicable to etiology diagnosis, serology detection and immunology detection and prevention of haemophilus parasuis diseases, and the enzyme-linked immuno sorbent assay (ELISA) antibody valence can reach 1:204800 after purification.

The invention belongs to the technical field of medicine production research development, and relates to isolation and identification as well as application of volatile oil elements of Parasenecio firmus leaf. The isolation and identification of the volatile oil elements of the Parasenecio firmus leaf are realized by the following steps of: weighing dried Parasenecio firmus leaf, and extracting ultrasonically by a water steam distillation method to obtain the volatile oil; and analyzing the obtain volatile oil by utilizing a 6890/5973N gas chromatograph-mass spectrometer. The obtain volatile oil mainly comprises the following chemical elements in proportion: 18.98% of n-palmitic acid, 9.89% of 1-tridecylene, 8.05% of caryophyllene epoxide, 6.04% of (S)-undeca-cycloolefin, 3.84% of 9,12,15-octadecatrienoic acid, 2.97% of (-)-spathulenol, 2.83% of kaurene and 2.12% of caryophyllene. The volatile oil is used for preparing pharmaceutical preparation capable of restraining escherichia coli, streptococcus, Salmonella, Pasteurella and staphylococcus aureus.

The invention relates to a new preparation technology and new applications of syringa amurensis rupr bark volatile oil, belonging to the technical field of research and development on medical products. The preparation technology comprises the steps of: drying and cutting up syringa amurensis rupr bark, soaking the syringa amurensis rupr bark in distilled water for 12 hours and conducting ultrasonic treatment for 30 minutes at temperature of 30 DEG C and with power of 80W; pouring medicinal materials together with soak solution into a volatile oil extractor and conducting extraction for 3 hours by adopting a water vapour distillation method; releasing water-yielding stratum and washing the volatile oil extractor repeatedly with ether so as to lead the syringa amurensis rupr volatile oil to be dissolved in the ether completely; and pouring the ether solution dissolved with volatile oil into a beaker, adding anhydrous sodium sulfate in the beaker to suck dry moisture and recycling the ether to obtain the purified volatile oil, and then storing the volatile oil in a refrigerator at temperature of 4 DEG C for standby. The syringa amurensis rupr bark volatile oil has inhibitory actions with different degrees on colon bacillus, staphylococcus aureus, pasteurella, streptococcus and salmonella, therefore the volatile oil can be developed into natural antibacterial agent used for industries of food and medicament, and the like, and prepare various products with antibacterial effects.

The invention discloses a traditional Chinese medicine preparation for preventing and treating livestock bowel diseases. According to the principle of treating both principal and secondary aspect of diseases, the traditional Chinese medicine preparation with double functions for healthcare is capable of preventing and treating livestock bowel diseases by strengthening the body resistance to eliminate pathogenic factors, suppressing bacterial growth in intestinal canals, killing intestinal bacteria and reducing damages to intestinal mucosa. On the basis of the secret prescription handed down from the inventor's ancestors and the traditional Chinese medicine theory, the traditional Chinese medicine preparation is prepared according to the monarch-minister-assistant-guide composition principle of a traditional Chinese medicine prescription, wherein clove, star anise, camuning, aspongopus and nasturtium are taken as monarchs, sward beans, saururus chinensis, common burreed rhizome, dried ginger, dried lacquer and pericarpium arecae are taken as ministers, liriope spicata, rhizoma kaempferiae, hawthorn leaves, semen cannabis and radix polygonati officinalis are taken as assistants, and juncus roemerianus, pepper, rice sprouts, sea-buckthorn, terminalia fruits and musk are taken as guides. The traditional Chinese medicine preparation which is prepared by scientific compositions and processes has high effects of killing and suppressing livestock escherichia coli, salmonella and pasteurella.

The invention relates to veterinary compound hydrochloric acid injection and a preparation method thereof. The veterinary compound hydrochloric acid injection consists of oxytetracycline dihydrate injection, imidocarb and diclofenac sodium; the veterinary compound hydrochloric acid injection per 100 ml comprises the required raw materials of: 10-25g of oxytetracycline dihydrate injection, 1.5-5g of diclofenac sodium, 0.1-0.2g of imidocarb, 0.2-0.6g of sodium formaldehyde sulphoxylate, 5-15g of magnesium chloride, 5-13 ml of ethanolamine and 60-81 ml of organic solvents, wherein the allowance is water for injection. The veterinary compound hydrochloric acid injection has remarkable effect when being used for treating acute respiratory infection caused by eperythrozoon suis, babesiosis and other blood protozoa diseases, porcine respiratory disease complex (PRDC) and swine influenza virus (SIV), airway inflammation induced by porcine reproductive and respiratory syndrome (PRRS), haemophilus parasuis, pasteurella, pleuropneumonia and mycoplasma diseases; and the preparation method is simple and easy to operate, and is suitable for batch production.

The present invention declaims the use of Pasteurella lipoprotein E (PlpE) as a subunit vaccine and the use of vaccines containing PlpE to protect animals from diseases caused by P. multocida. The results of vaccination and challenge experiments showed that mice and chickens immunized with PlpE were completely protected animals from challenge infection with 10¹-10³ LD₅₀ of P. multocida and no adverse effect was observed.

The invention belongs to the technical field of pharmaceutical chemosynthesis, and particularly relates to quinoxaline-N1,N4-dioxide derivatives with antimicrobial activity. The invention also relates to preparation of the derivatives and antimicrobial activity testing of the derivatives. The new synthetic compounds are prepared by the following steps: carrying out Beirut reaction on the raw material N-benzofuroxan oxide and malononitrile under alkaline conditions to obtain 3-amino-2-cyano-quinoxaline-N1,N4-dioxide, and reacting with appropriate acyl chloride to obtain a series of 2-cyano-amidoquinoxaline-N1,N4-dioxides. The in-vitro antibacterial activity test result indicates that the quinoxaline-N1,N4-dioxide derivatives have favorable antibacterial activity for mycobacterium hominis and mycobacterium bovis and also have antibacterial activity for Staphylococcus aureus, Streptococcus pneumoniae and Pasteurella. The invention also discloses a structural formula of the compounds used as a target antibacterial drug.

The invention relates to a bacilli, its compound preparation and the preparing method for the compound preparation. Its name is Bacillus subtilis LY-35, which is preserved in the general microbe center of Micro Germ conservation Management committee of China, and its conservation number is:CGMCC N0.1222. The preparation is a mixture of lambda phage of culture of bacilli and staphylococcus, streptococcus, colibacillus, bacillus proteus, pseudomonad, salmonella, pasteurella and klebsiella, can cure and prevent all kinds of illness caused by conditional pathogen,is a micro biological preparation which can be used by being mixed with antibiotics or replace antibiotics without any remainder and pollution, with good curative effect and safety.