11 results about "Pasteurella" patented technology

The invention discloses a pasteurella phage PTP1APTYT15 as well as a composition, a kit and application of the pasteurella phage PTP1APTYT15, the pasteurella phage PTP1APTYT15 is preserved in Guangdong Microbial Culture Collection Center on September 10, 2025, the preservation number of the pasteurella phage PTP1APTYT15 is GDMCC NO: 66946-B1, and the pasteurella phage PTP1APTYT15 is classified and named as Pasteurella phage PTP1APTYT15. The pasteurella phage PTP1APTYT15 provided by the invention has the characteristics of high temperature resistance and alkali resistance, and can be used for cracking pasteurella of various serotypes. In addition, the bacteriophage is high in proliferation efficiency and high in splitting capacity, has no adverse effect on human or animal health, and has good biological safety.

This invention discloses a method for preparing rum with high total ester content. The method includes the following steps: pretreating molasses; preparing a first fermentation medium using the pretreated molasses; expanding the culture of fissile yeast seed liquid in the first fermentation medium to obtain fissile yeast fermentation broth; continuously adding the pretreated molasses to the fissile yeast fermentation broth, culturing to obtain a fermentation product, wherein the residual sugar content of the fermentation product is controlled at 0.5-2%; preparing a second fermentation medium using the pretreated molasses; inoculating the fermentation bacteria into the second fermentation medium, culturing to obtain a fermentation broth; the fermentation bacteria include at least one of Clostridium acetic acid and Acetobacter pasteurella; adding the fermentation broth to the fermentation product, culturing to obtain a mash; continuously distilling the mash, using a kettle heating method for three-part distillation, mixing the second fraction with oak shavings, and aging; obtaining the base spirit, which is then blended to obtain rum with high total ester content. This method can produce mash with high alcohol content and high ester content in a short time.

The invention relates to the technical field of molecular biological detection, and particularly discloses a sheep pasteurella serum A type, B type and F type triple PCR detection method, a kit and application thereof. According to the method, three pairs of specific primers are respectively designed for serum A type, B type and F type sheep pasteurella, extracted sheep pasteurella DNA is taken as a template, a PCR amplification system is constructed by the specific primers for triple PCR amplification, negative and positive control is provided in detection, single detection of pasteurella serum A type, B type and F type can be rapidly and accurately detected, and detection of the serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum B type and F type of pasteurella serum A type, B type and F type of pasteurella serum B type and F type of pasteurella serum B type can be rapidly and accurately detected. The B type and F type can be simultaneously detected, the accuracy of serum typing is greatly improved, the occurrence probability of false positive is reduced, the specificity is relatively strong, and the kit can be used for pasteurella serotype general survey, molecular epidemiological investigation and vaccine serotype screening and detection.

The invention discloses a pasteurella phage PTP2APTNM6 as well as a composition, a kit and application of the pasteurella phage PTP2APTNM6, the pasteurella phage PTP2APTNM6 is preserved in Guangdong Microbial Culture Collection Center on September 10, 2025, the preservation number of the pasteurella phage PTP2APTNM6 is GDMCC NO: 66945-B1, and the pasteurella phage PTP2APTNM6 is classified and named as Pasteurella phage PTP2APTNM6. The pasteurella bacteriophage PTP2APTNM6 provided by the invention can specifically recognize a pasteurella serum A-F type strain, the total splitting rate reaches 90%, and the pasteurella bacteriophage PTP2APTNM6 has good high temperature resistance and acid and alkali resistance stability.

This invention discloses a method for preparing and applying a Pasteurella-Bordetella nanovaccine, belonging to the field of biomedical technology. The method includes the following steps: emulsifying epigallocatechin gallate, an antigen aqueous phase, and soybean oil under high-speed shearing, followed by homogenization to form a stable oil-in-water emulsion, which is the Pasteurella-Bordetella nanovaccine. Challenge experiments confirmed that the ESO vaccine achieved a protection rate of up to 90% against Pasteurella multocida and Bordetella bronchiseptica, significantly superior to the 40%-70% protection rate of the aluminum adjuvant group. Simultaneously, it significantly reduced the bacterial load in the lungs, demonstrating excellent clinical protective effects. This invention, through the innovative combination of EGCG and vegetable oil, overcomes the limitations of traditional adjuvants in terms of immunogenicity, safety, and single function, providing a highly efficient and safe new vaccine solution for the prevention and control of Pasteurella multocida and Bordetella multocida infections.

The invention discloses a biogas production method based on an enzyme-bacterium compound preparation and fruit waste, and belongs to the technical field of agricultural waste resourceful treatment. The production method comprises the following steps: mixing and crushing fruit wastes into particles with the particle size of 1-5mm to obtain a mixed material; mixing with ultrapure water, adjusting the pH to be acidic, and heating in a water bath to obtain a mixed solution; cooling, adjusting the pH value, adding an enzyme-bacterium compound preparation, fermenting, centrifuging, and discarding the precipitate to obtain a fermentation liquid; adjusting the pH value to 6.8-7.5, transferring into an anaerobic fermentation tank, adding methanosarcina pasteurii, and carrying out secondary fermentation to obtain fermentation gas; and leading out from the anaerobic fermentation tank, and sequentially carrying out gas-liquid separation, desulfurization and dehydration treatment to obtain pure biogas. According to the method, the fruit waste is converted into high-quality clean energy, the biogas yield and the methane content are remarkably increased, and an efficient and reliable new way is provided for resourceful treatment of fruit processing byproducts.

The invention belongs to the technical field of pathogenic microorganism screening and aquaculture disease prevention and control, and discloses an aeromonas salmonicida natural attenuated strain derived from channel catfish and application of the aeromonas salmonicida natural attenuated strain, and the preservation number of the strain is CCTCC NO: M20252718. Through artificial infection, the toxicity of the strain on tested rainbow trout and grass carp is far lower than that of an aeromonas salmonicida virulent strain, and a live vaccine prepared from the strain is used for performing immune protection on rainbow trout and grass carp, so that bacterial diseases caused by the aeromonas salmonicida virulent strain can be effectively prevented. In the culture process, the strain can effectively reduce the use of antibiotic drugs, and has a positive promotion effect on reducing drug residues of freshwater fishes and further realizing green culture.

The invention provides a pig lung pasteurization disease management system and method, electronic equipment and a medium, and the system comprises an image obtaining module which is used for obtaining a plurality of groups of original pig lung images; the image processing module is used for performing image processing on each group of original pig lung images to obtain a target lung image; the image recognition module is used for recognizing the target lung image based on a pre-trained Pasteur disease recognition model to obtain a Pasteur disease recognition result; and the result analysis module is used for analyzing the pasteurization disease recognition result and determining a breeding scheme based on the analysis result. The method can improve the recognition efficiency, saves the manpower of manual evaluation, and improves the health level of pig breeding.

This invention relates to the field of building materials technology and addresses the problem of freeze-thaw cracks in building exterior walls in extremely cold regions. The repair materials include butyl acrylate, glycidyl methacrylate, ethyl p-methoxycinnamate, PEG-S-S-PEG, N-TiO2@PANI composite particles, Bacillus pasteurella microcapsules, nutrient salt microcapsules, and the photoinitiator HMPP. The building exterior wall materials also include quartz sand, shale ceramsite, P·O 42.5 cement, β-hemihydrate gypsum powder, polycarboxylate superplasticizer, hydroxypropyl methylcellulose, ethylene glycol, silicone defoamer, nano-calcium carbonate, and chopped cellulose fibers. This invention can reduce overall maintenance costs, improve structural safety, and is suitable for the needs of buildings in northern regions.

This invention relates to the field of building materials, specifically to a steel slag-based low-carbon ultra-high-performance concrete composite material and its preparation method. The composite material comprises, by weight, 30-50 parts mineralized steel slag powder, 20-30 parts cement, 10-15 parts silica fume, 5-10 parts fly ash, 1-3 parts water-reducing agent, 15-25 parts steel fiber, and 10-15 parts water. The mineralized steel slag powder is obtained by biochemical mineralization of steel slag powder through a mixture of alkaline bacterial solution containing Bacillus pasteurella and a calcium source substrate. A calcium carbonate crystal layer is deposited in situ on its surface, and residual bacterial protein adheres to the pores, forming a multi-scale stacked cementitious skeleton with silica fume and fly ash. The preparation includes mineralization, dry mixing, slurry preparation, fiber addition, and curing steps. This invention eliminates the free calcium oxide expansion source, preventing matrix cracking, fills the pores on the steel slag surface, and strengthens the interfacial bonding strength.

The invention relates to the technical field of microorganisms, in particular to a prenatal suicide idea marker which comprises intestinal flora, the intestinal flora comprises bacteroides, flavonolytic bacteria, pasteurella, unclassified bacteria of the family of hispiraceae, unclassified bacteria of the family of tremoraceae, actinomycetes, twin coccus, unclassified bacteria of the family of streptococcaceae and auribacter. By detecting the abundance of the flora marker in a fecal sample of the pregnant woman, the prenatal suicide idea risk of the pregnant woman can be effectively diagnosed, a new perspective is provided for the research of a potential mechanism of the suicide idea, and the diagnosis method has the advantages of high specificity, high sensitivity, high accuracy, simple operation, safety, non-invasive property, low medical cost, low missed diagnosis rate and wide application prospect. The method is beneficial to timely intervention and inhibition of suicide mind of women during pregnancy, improves physical and psychological health of mothers and infants, provides accurate intervention guidance for clinic, and is suitable for clinical popularization and application.