The invention relates to a method for preparing a human antithrombin-III product, which comprises the following steps of: separating a component IV in a plasma protein process by using a Cohn low-temperature ethanol method, precipitating the component IV serving as a raw material; and finally, preparing the high-purity antithrombin-III lyophilized product through extracting, filter pressing, carrying out heparin affinity chromatography, ultra-filtering, degerming, filtering, lyophilizing and the like and a necessary S/D and dry heat virus inactivating process. According to the invention, the process is adjusted, so that AT-III is dissolved as much as possible; the Pasteur virus inactivating process is replaced by the S/D virus inactivating process by adding proper protective agent; activity loss is reduced; the preparation time is shortened; furthermore, high-purity AT-III solution can be obtained only in the need of heparin affinity chromatography for one step; after carrying out the steps of ultra-filtering, freezing, drying, inactivating dry heat virus and the like, the purity of a final product is above 95%; and the specific activity is above 6.5 IU/mg and is 3.0 IU/mg more than specified limits in the European pharmacopoeia and united state pharmacopoeia.