11results about How to "Simplified purification steps" patented technology

The invention provides a centrifugal collecting device. The centrifugal collecting device comprises a driving part and a driving part, the rotor assembly is connected with the driving piece; the liquid storage part is arranged on the rotor assembly, and the liquid storage part is provided with a liquid storage cavity; the flow guide assembly comprises a flexible flow guide pipe, and one end of the flexible flow guide pipe is connected with the liquid storage part and communicates with the liquid storage cavity; the flow adjusting part is connected with the rotor assembly, a slit is formed in the flow adjusting part, and under the condition that the flexible flow guide pipe is embedded into the slit, the flexible flow guide pipe deforms; the collection assembly comprises a treatment container, a filter part and a collection container, the filter part is arranged in the treatment container and divides the treatment container into a treatment chamber and a confluence chamber, the treatment chamber is communicated with the other end of the flexible flow guide pipe, and the confluence chamber is communicated with the collection container, that is, the effective stripping of the adsorbed secretions is realized; the dosage of a buffer solution is remarkably reduced, and the concentration of effective components in a collected solution is greatly improved, so that a detection result is closer to the content of real secretions in soil.

The application discloses lignin carbon quantum dot fluorescent bamboo fiber, a preparation method and application thereof, and relates to the technical field of bamboo fiber, in particular to a lignin carbon quantum dot fluorescent bamboo fiber, a preparation method and application thereof. The preparation method of the lignin carbon quantum dot fluorescent bamboo fiber comprises the following steps: selecting a rough planed bamboo strip sample, drying the bamboo strip sample at 80-90 DEG C until the moisture content is less than 30%, and sequentially cleaning the surface of the bamboo strip sample by using anhydrous ethanol and acetone, and then preparing the bamboo strip sample; mixing choline chloride, oxalic acid and deionized water and stirring uniformly to prepare a delignification aqueous solution; and the like. The lignin carbon quantum dot fluorescent bamboo fiber, the preparation method and the application thereof separate and treat bamboo cells, obtain bamboo fibers with high strength, use alkali lignin as a carbon source, hydrogen peroxide as an oxidant, and a small amount of p-phenylenediamine as a nucleating agent, successfully prepare lignin carbon quantum dots with yellow-green fluorescent emission by using a hydrothermal method, and combine the lignin carbon quantum dots with the bamboo fibers to prepare carbon quantum dot fluorescent bamboo fibers with fluorescent properties, and the carbon quantum dot fluorescent bamboo fibers have certain application prospects in the fields of anti-fake marks and intelligent textiles.

This invention belongs to the field of biomedical technology, specifically relating to a human stem cell factor encoding gene, a recombinant expression vector, an engineered strain, and a preparation method thereof. The nucleotide sequence of the encoding gene is shown in SEQ ID NO:1. The recombinant expression vector includes an expression vector and the encoding gene shown in SEQ ID NO:1. The engineered strain is obtained by transforming the recombinant expression vector containing the encoding gene into a host strain and screening. This invention also provides a method for preparing human stem cell factors, comprising: (1) culturing the engineered strain in a culture medium until the OD reaches a suitable range; (2) obtaining a fermentation broth through induced culture; and (3) purifying and separating the fermentation broth to obtain recombinant human stem cell factors. This invention optimizes the protein expression system and purification process while ensuring the acquisition of high-purity human stem cell factors, making it more suitable for industrial-scale production and providing a reference for achieving large-scale and efficient preparation.

This invention provides a method and application for transient expression of collagen in plants. The collagen fragment C3S1, C3S2, C3S3, C3S4, and C3S5 are linked together with the amino acid sequences shown in SEQ1, SEQ2, SEQ3, SEQ4, and SEQ5, respectively. This collagen fragment can be expressed using tobacco as a bioreactor. By designing expression vectors, its expression effect in tobacco can be compared, and a transient expression system in tobacco can be established to achieve efficient expression of the collagen fragment in tobacco. This preparation method can express recombinant collagen fragments in tobacco with high expression levels. Compared to full-length intron-modified collagen or expression using type I collagen, this method achieves efficient expression of type III collagen fragments in tobacco bioreactors. It has a short cycle time, requires no maintenance of cell lines and bioreactors, significantly reduces production costs, and has a high safety index.

This invention discloses a method for the direct oxidative carbonylation of methane to prepare acetic acid. Specifically, this invention provides a catalytic method for the preparation of acetic acid from methane, comprising the following steps: under solvent conditions, in the presence of light, an iron catalyst, chloride ions, and an oxidant, methane and carbon monoxide undergo a methane oxidative carbonylation reaction to obtain acetic acid. This method utilizes readily available methane, carbon monoxide, and air (or oxygen), employs an iron catalyst, and achieves direct, efficient, and highly selective acetic acid production via a one-step photocatalytic reaction, demonstrating promising application prospects. The substrate scope of this invention is broad, applicable to a range of chain and cyclic alkanes.

The application discloses givinostat and a synthesis method thereof, wherein compound 1 is used for synthesizing compound 2 in step 01; compound 3 is used for synthesizing compound 4 in step 02; compound 4 is used for synthesizing compound 5 in step 03; compound 5 is used for synthesizing compound 6 in step 04; and compound 6 and compound 2 are used for synthesizing givinostat in step 05. After optimization, the yield of compound 1 to compound 2 reaches more than 80 %, raw materials and reagents used in the reaction are cheap and easy to obtain, and the preparation cost is very low; only four steps are needed from compound 3 to the target product, the route is short, and the operation is simple; and after optimization, the total yield of compound 3 to the target product reaches more than 40 %, the yield is high, and the preparation cost is significantly reduced.

The invention discloses a nucleic acid in-situ detection system based on CRISPR / dCas fusion protein and TSA signal amplification and application of the nucleic acid in-situ detection system based on CRISPR / dCas fusion protein and TSA signal amplification, and the nucleic acid in-situ detection system based on CRISPR / dCas fusion protein and TSA signal amplification comprises fusion protein based on a CRISPR / dCas system and a tyramine solution modified by a fluorophore, the fusion protein based on the CRISPR / dCas system is at least one of the following components: dCas9-HRP or biotinylated dCas9-Avi Tag, dCas9-HRP, dCas9-Avi Tag, dCas9-HRP and dCas9-Avi Wherein the dCas9-HRP has a sequence as shown in SEQ ID NO: 1 or a functional variant of the sequence as shown in SEQ ID NO: 1; the biotinylated dCas9-Avi Tag has a sequence as shown in SEQ ID NO: 2 or a functional variant of the biotinylated dCas9-Avi Tag.

The application discloses a method for preparing gamma-cyclodextrin by precipitation technology and enzyme method, and comprises the following steps: taking gamma-cyclodextrin mother liquor prepared by a solvent method and enzyme method as a sample solution; under high-temperature conditions, first, a complex formed by the gamma-cyclodextrin and a reaction aid is dissociated; then, a precipitation separation technology is used; and finally, an inorganic salt precipitant is added to effectively separate a reaction agent, so that the purification of a target product is realized, and a high-efficiency and simple gamma-cyclodextrin separation and purification method is established. By using the method, the total conversion rate of the gamma-cyclodextrin can reach about 53.89%, the recovery rate of the gamma-cyclodextrin after separation can reach 82.76% to 90.84%, the purity is 92.56% to 95.63%, and the residual amount of the reaction aid is 0.02%.

The application provides a viscosity-responsive luminescent probe, a preparation method and application. The luminescent probe has an ultra-sensitive viscosity response performance, and the viscosity response range thereof covers the viscosity change range of edible oil. After the luminescent probe is added into the edible oil, the fluorescence intensity has a high correlation with the content of the polar component in the edible oil. The change of the polar component of the edible oil is obtained by using the response of the luminescent probe to the viscosity change of the edible oil, so that the rapid and sensitive detection of the polarity of the frying oil is realized.

The application discloses a kind of metal organic framework materials and preparation method and application thereof.The metal organic framework material provided by the application includes metal ions and organic ligand, and the organic ligand is selected from the compound shown in formula I or formula II.The metal organic framework material of the application has excellent adsorption separation selectivity to p-xylene.The raw material for synthesizing the material is cheap and easy to obtain, the material preparation process is simple and low in cost, and the material has good regeneration and repetition performance, and can maintain the original adsorption effect after vacuum or heating regeneration, and has wide industrial application prospect.

The present application relates to the field of bioengineering, and particularly relates to a mutant of mu-conotoxin and a preparation method and an enzyme composition thereof.The present application provides a mutant of mu-conotoxin, which has an amino acid sequence as shown in SEQ ID NO:1, or an amino acid sequence obtained by substituting, deleting or adding one or more amino acids in the amino acid sequence, and an amino acid sequence which is functionally identical or similar to the amino acid sequence, or an amino acid sequence which is at least 80% identical to the amino acid sequence.The technical route and process provided by the present application greatly improve the production efficiency and quality of mu-conotoxin, are suitable for industrial large-scale production, and have a wide application prospect.