27 results about "Oviduct" patented technology

The invention is applicable to the technical field of rabbit breeding, and provides a method for increasing the body weight of a rabbit based on a gene editing technology, which comprises the following steps: designing an action target sgRNA on a rabbit NR6A1 gene aiming at an NR6A1 p.P192S site; uniformly mixing the synthesized sgRNA and SpRY-CBE, injecting fertilized eggs, transplanting the fertilized eggs into fallopian tubes of receptor female rabbits, and feeding the receptor female rabbits until production; dNA is extracted from produced young rabbit ear tissues, PCR and sequencing are carried out, and if C at the fifth site in NR6A1 gene target sites is mutated into T, it is proved that single base mutation is obtained, and the NR6A1 gene edited rabbit is obtained. According to the invention, the rabbit variety with increased weight is successfully cultivated; a new thought of animal genetic breeding is developed by utilizing a gene editing technology, the method has application values in promoting improvement of genetic characters of rabbits and improving production performance and economic value of the rabbits, and powerful support is provided for high-quality development of the animal breeding industry in China.

The invention relates to a gene point mutation mouse model construction method. The method comprises the following steps: designing sgRNA and ssODN by taking T274M mutation on Kcnq2Exon6 as a target spot; the method comprises the following steps: selecting a female mouse at a set week age as an ovum donor, injecting a certain dose of serum gonadotropin and human chorionic gonadotropin into the abdominal cavity of the female mouse, mating with a breeding male mouse, and collecting fertilized ova of the mated female mouse; injecting Cas9 protein, ssODN and sgRNA into the pronucleus of the fertilized ovum through a micro-injection technology, transplanting the fertilized ovum after injection into the oviduct pot belly of the female rat to be pregnant, and obtaining a newborn rat after the female rat to be pregnant is delivered; according to a Kcnq2 gene point mutation site, a pair of identification primers F1 and R1 are designed at the two ends of the mutation site respectively, and a newborn mouse is identified, so that a Kcnq2T274M / + point mutation mouse with T274M mutation successfully knocked in is screened out. The Kcnq2T274M / + point mutation mouse editing method is simple in preparation and high in repeatability, and a larger number of Kcnq2T274M / + point mutation mice can be obtained while the editing efficiency is improved.

The invention relates to the technical field of veterinary biological products, and particularly discloses riemerella anatipestifer cRA / HeN2024, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.32898, the preservation date is December 4, 2024, the preservation unit is China General Microbiological Culture Collection Center, and the preservation address is Yard 1, Beichen West Road, Chaoyang District, Beijing. The riemerella anatipestifer cRA / HeN2024 is type 1 riemerella anatipestifer and is separated from fallopian tube exudate of 15-day-old sick chicken showing respiratory tract symptoms; the isolated strain has a far genetic evolution relationship with the existing duck-origin and goose-origin riemerella anatipestifer, and the inactivated vaccine prepared from the strain has excellent immunogenicity and can effectively prevent and treat air sacculitis, salpingitis and pericarditis after chicken is infected with RA.

The application relates to the technical field of traditional Chinese medicine preparation, and particularly discloses a compound traditional Chinese medicine preparation for targeting egg duck and goose egg cytoplasmic disease, which comprises the following components: white head, Coptis chinensis, Huangqi, Phellodendron amurense, wine Danpi, bran-fried peach kernel, Chinese perilla, pomegranate peel, liquorice, honeysuckle, Andrographis, figwort, emblic, ginger, fried atractylodes, fried safflower, Xianhecao, spiny amaranth, and Poria cocos. Through the combination of monarch, minister, assistant and messenger, the core effects of heat-clearing and detoxifying, blood-cooling and dysentery-stopping, and dampness-drying and insect-killing of the medicines are synergistically exerted, the pathogenesis characteristics of the egg cytoplasmic disease are accurately targeted, blood stasis and blood circulation are promoted to eliminate the swelling of the lesion, and the intestinal mucosa damage is repaired to stop bleeding, the symptoms of hematochezia, diarrhea and oviduct inflammation of the egg duck and goose caused by the egg cytoplasmic disease are effectively relieved, the problems of the decreased antibiotic effect, drug residue, bacterial flora disorder and food safety risk are effectively solved, and the compound traditional Chinese medicine preparation has the remarkable progress of drug resistance, safety, no residue, intestinal protection and green environmental protection.

The application provides a monoclonal antibody of a Chlamydia trachomatis Pgp3 protein and application thereof, and the monoclonal antibody comprises a heavy chain variable region CDR1, a heavy chain variable region CDR2 and a heavy chain variable region CDR3 with the amino acid sequences shown in SEQ ID NO. 1, 2 and 3 respectively, and a light chain variable region CDR1, a light chain variable region CDR2 and a light chain variable region CDR3 with the amino acid sequences shown in SEQ ID NO. 4, 5 and 6 respectively. Experimental researches prove that the monoclonal antibody of the Chlamydia trachomatis Pgp3 protein can effectively inhibit infection of Chlamydia trachomatis in a mouse genital tract, and reduce occurrence of oviduct edema.

This invention belongs to the field of agricultural biotechnology, specifically relating to locusts. LARP4B Genes, their dsRNAs, and applications. To provide novel target genes for green pest control, this invention utilizes bioinformatics methods to obtain genes from the transcriptome of locusts. LAPR4B The gene fragment was further cloned and sequenced, yielding the sequence SEQ ID NO: 1. Based on SEQ ID NO: 1, the dsRNA of this gene was designed and synthesized. The nucleotide sequence of this gene fragment is shown in SEQ ID NO: 2. Injecting it into the locust's body cavity specifically silences the target gene, inhibiting the development of the locust's ovary, resulting in a smaller ovary and shorter, narrower oviducts, thereby effectively suppressing the locust population. Due to the specificity of this invention and its effect on ovarian development, it has significant practical implications for suppressing locust populations and can provide a new approach for pest control.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

The invention relates to the technical field of veterinary fallopian tube production, in particular to veterinary fallopian tube tail plug assembling equipment which comprises a supporting frame, a vibration feeding disc, a linear vibrator, a mounting plate, a conveying disc and a top plate, the vibration feeding disc, the linear vibrator and the mounting plate are mounted at the top of the supporting frame, and the conveying disc is mounted at the top of the linear vibrator; a fixing plate and a collecting box are fixed to the top of the mounting plate, a top plate is fixed to the top of the mounting plate through a supporting rod, an extending plate is fixed to the top of the top plate, a straight supporting plate is mounted at the top of the mounting plate, a horizontal plate is mounted at the top of the straight supporting plate, and a plurality of butt joint blocks are fixed to the top of the horizontal plate. The overturning motor drives the rotating plate to rotate by 90 degrees, the ten eighth air cylinders on the rotating plate drive the ten sets of clamping clamps to clamp the lock catches correspondingly, the overturning motor drives the clamping clamps and the lock catches to overturn by 90 degrees, the lock catches are overturned to be horizontal, overturning work of the ten lock catches can be completed synchronously, and the assembling machine has the advantage of being high in assembling efficiency.

The invention discloses a human immortalized fallopian tube fibroblast strain as well as a construction method and application thereof, and belongs to the technical field of cell immortalization construction. The preparation method comprises the following steps: firstly, separating, culturing and purifying fibroblasts from human fallopian tube tissues by virtue of an enzyme digestion method, identifying markers of the fibroblasts, then introducing a simian virus 40 large T antigen gene into primary fibroblasts by virtue of a lentiviral vector, and screening by virtue of puromycin to obtain a cell strain capable of stably expressing SV40T. Finally, the human immortalized fallopian tube fibroblast which can be stably passaged is constructed. The cell strain has the characteristics of strong multiplication capacity and high hereditary stability, and the cells are not obviously aged along with the increase of the number of generations of the cells, which indicates that the human immortalized fallopian tube fibroblast line is successfully established. According to the invention, the problem that the in-vitro culture life of primary fibroblasts is limited is solved, and a reliable in-vitro cell model is provided for research in the fields of fallopian tube related diseases, ovarian cancer origin, tumor microenvironment and the like.

Provided is a method for constructing a humanized FKBP8 gene knock-in animal model, comprising: (1) constructing a targeting vector for humanized FKBP8 gene knock-in, and transfecting the targeting vector, Cas9, and sgRNA into fertilized eggs of donor animals; (2) transplanting surviving fertilized egg cells into the oviduct of pseudopregnant animals to obtain F0 generation animals and identifying the F0 generation animals, wherein animals showing positive homologous recombination are chimeric animals; and (3) mating the chimeric animals with wild-type animals to obtain F1 generation animals and performing identification, wherein F1 animals showing positive homologous recombination are humanized FKBP8 knock-in animals. In the construction method, an FKBP8 gene sequence in a donor animal is substituted by a human FKBP8 gene sequence, so as to achieve the purpose of humanizing the FKBP8 gene in the donor animal, thus successfully establishing a humanized FKBP8 gene knock-in animal model, so that screening for drugs targeting the FKBP8 gene and a product thereof in the animal model, or studying the pathogenesis and treatment mechanisms of the FKBP8 gene and the product thereof in related clinical diseases can be realized.

The application provides a pharmaceutical composition for treating oviduct inflammation and peritonitis of laying hens and a preparation method thereof, and belongs to the technical field of veterinary drugs. The pharmaceutical composition is obtained by mixing 2-10 parts of artemisinic acid, 1-10 parts of marrubiin hydrochloride, 1-10 parts of sodium salicylate, 0.001-0.005 parts of imipenem / cilastatin sodium and auxiliary materials. The pharmaceutical composition can treat oviduct inflammation, peritonitis and intestinal secondary infection of laying hens through anti-pathogenic microorganism infection and anti-inflammatory pathways, and significantly improves the production performance of laying hens. After the chickens are medicated, the effect is fast, the egg production rate is obviously increased, and the substandard egg rate is obviously decreased. The oviduct inflammation and peritonitis of laying hens over 300 days old can be effectively solved, the clinical use is safe and reliable, the meat and egg food quality and safety can be improved, and it is particularly important for large-scale laying hen farms.

The application discloses a culture solution for improving the blastocyst rate and quality of bovine embryos, application and an in-vitro culture method, and relates to the technical field of animal embryo engineering. The application provides a culture solution for in-vitro culture of bovine embryos, which comprises a first culture solution and a second culture solution with different osmotic pressure concentrations. By controlling the osmotic pressure concentrations of the first culture solution and the second culture solution in a suitable range, and by using the first culture solution in the early stage of embryo development and the second culture solution in the late stage of embryo development, the in-vitro culture system can better simulate the environment in the oviduct in the early stage of embryo development and better simulate the environment in the uterus in the late stage of embryo development, so that the osmotic pressure stress of the embryo in the development process is reduced, and the blastocyst rate and the blastocyst quality of the bovine embryo are improved. Compared with a traditional culture system with constant osmotic pressure, the application significantly improves the blastocyst rate by sequential regulation of the osmotic pressure, the total cell number of the obtained blastocysts is more, and the apoptosis index is lower.

The present invention is applicable to the field of rabbit breeding technology and provides a method for increasing rabbit weight based on gene editing technology, comprising: designing a target sgRNA for the NR6A1 p.P192S site on the rabbit NR6A1 gene; mixing the synthesized sgRNA and SpRY‑CBE, injecting the resultant sgRNA into a fertilized egg, transplanting the mixture into the oviduct of a recipient female rabbit, and raising the recipient female rabbit until birth; extracting DNA from the ear tissue of the resulting young rabbit, performing PCR and sequencing, and if the C at position 5 in the NR6A1 gene target site mutates to T, a single base mutation is obtained, i.e., an NR6A1 gene-edited rabbit is obtained. The present invention successfully breeds a rabbit breed with increased weight; utilizing gene editing technology to develop new ideas for animal genetic breeding, has application value in promoting the improvement of rabbit genetic traits, enhancing rabbit production performance and economic value, and provides strong support for the high-quality development of my country's animal seed industry.

The application relates to the technical field of animal model construction, and particularly discloses a Sypl1 gene knockout colorectal cancer mouse model construction method and application, which comprises the following steps: synthesizing a specific target site gRNA of a Sypl1 gene, mixing Cas9 protein and the target site gRNA to obtain an injection compound, microinjecting the injection compound into mouse zygotes, and transplanting the surviving zygotes into the oviducts of pseudopregnant female mice to obtain F0 generation mice, and the mice born after 20 days are Sypl1 gene knockout mouse animal models; and a chemical induction modeling method is used to construct a colorectal cancer model in wild type mice and gene knockout mice. The Sypl1 gene knockout mouse is constructed by using a CRISPR / Cas mediated genome engineering technology Sypl1 combined with the most widely used colorectal cancer chemical inducer azoxymethane / dextran sodium sulfate, a new colorectal cancer mouse model is constructed, compared with a traditional chemical induction model, the tumor formation rate can be significantly improved, and the tumor formation time can be shortened, and a more efficient animal model is provided for colorectal cancer research.

The invention discloses a method for rapidly constructing a Foxn1 homozygous nude mouse through RepSox induction based on CRISPR / Cas9, and the method comprises the following steps: synthesizing two sgRNAs for respectively identifying a 5'terminal target site and a 3 'terminal target site, the nucleotide sequence of the sgRNA for identifying the 5' terminal target site is as shown in SEQ ID NO: 1, and the nucleotide sequence of the sgRNA for identifying the 3 'terminal target site is as shown in SEQ ID NO: 2; a mixed solution of the two sgRNAs, the Cas protein and the RepSox is microinjected into the fertilized eggs of the rats; the injected embryos are subjected to transient in-vitro culture, survival embryos are screened and transplanted into fallopian tubes or uteruses of pseudopregnant female rats for development, and F0-generation Foxn1 knockout homozygous rats are obtained. According to the method, the Foxn1 gene can be accurately knocked out, the survival rate of a gene editing embryo is improved, the model construction period is shortened, and the rat knockout efficiency is remarkably improved.

The invention provides a feed additive for preventing and controlling poultry salpingitis as well as a preparation method and application of the feed additive, and relates to the technical field of poultry feed additives. The feed additive provided by the invention is prepared by taking a plant source functional component, a prebiotic component, a polyphenol-essential trace element chelate, an essential amino acid balance component, a vitamin and antioxidant component and a high-viable-count probiotic freeze-dried powder component as core components through scientific proportioning and a vacuum low-temperature drying technology. Through cooperation of the multiple components, the poultry intestinal micro-ecological balance is systematically adjusted, and secondary salpingitis caused by the'intestinal tract-reproductive axis' way is inhibited from the source; when the feed additive is applied, 0.5%-6% (preferably 3%-5%) of the feed additive is added into basic ration, and animal experiments show that the feed additive can effectively reduce the number of pathogenic enterobacteria and ammonia nitrogen emission, significantly reduce the occurrence rate (Plt, 0.001) of salpingitis, significantly improve the laying rate and the quality of egg products, and achieve the breeding goals of efficient resistance replacement, safety and environmental protection.

A system for treating a female patient to promote pregnancy comprising a restriction device adapted to postoperatively restrict and release an oviduct of the patient.

The present application relates to the technical field of exosome, and particularly discloses a stable production method of exosome for promoting bovine embryo development and application thereof. The exosome is prepared by a bovine endometrial epithelial cell line or a bovine oviduct epithelial cell, and stable, large and pure exosome can be obtained, which can be used for large-scale production. Meanwhile, the addition of 100 ug / ml of exosome into the culture medium at the 16-cell stage of bovine embryo development can significantly improve the blastocyst rate and blastocyst quality, and provide support for in vitro culture of embryos.

The invention discloses lactobacillus reuteri, which is characterized in that the lactobacillus reuteri can be used for preparing lactobacillus reuteri, and the lactobacillus reuteri can be used for preparing lactobacillus reuteri. The lactobacillus reuteri strain is preserved in the General Microbiological Culture Collection Center (GCMCC) of the China Committee for Culture Collection of Microorganisms, and the preservation number is CGMCC No. 34177; the strain can penetrate through the intestinal tract-fallopian tube-ovary of a laying hen, improve microbiota of each part and improve the ovarian function of the laying hen. The invention also discloses an application of the feed containing Lactobacillus reuteri in the feed for increasing the laying rate of laying hens. Experimental results show that the laying rate of laying hens is obviously increased.

The invention discloses a construction method and application of a mouse model based on reverse insertion of an HPV16 gene, and belongs to the technical field of animal models. According to the method, a CRISPR (clustered regularly interspaced short palindromic repeats) / Cas9 mediated genome editing technology is utilized, and directional knock-in of an HPV16 sequence is realized at a Klf5 gene locus of a C57BL / 6J mouse. The method comprises the following steps: (1) designing and synthesizing specific gRNA; (2) constructing a targeting carrier Donor vector; and (3) micro-injection: mixing Cas9 protein, gRNA and a targeting carrier Donor vector to prepare an RNP injection compound, micro-injecting the RNP injection compound into a mouse fertilized egg, and sending the RNP injection compound back to a pregnant mouse fallopian tube to cultivate offspring so as to generate a gene knock-in mouse. The animal model can simulate a cervical cancer precancerous lesion process caused by HPV persistent infection, and provides a reliable experimental basis for cervical cancer occurrence mechanism research and anti-HPV drug screening.

The invention discloses an application of clostridium butyricum in preparation of a product for relieving oviduct injury of laying hens caused by vomitoxin. The clostridium butyricum is clostridium butyricum BNCC337239. The clostridium butyricum can effectively relieve the vomitoxin-induced fallopian tube injury of laying hens, it is proved that the clostridium butyricum as a probiotic can relieve the toxic action of vomitoxin, and a new view is developed for functional research of the clostridium butyricum. The clostridium butyricum serves as a feed additive and is applied to prevention and treatment of chronic poisoning of laying hens caused by vomitoxin, new application of the clostridium butyricum is developed, and a new solution is provided for prevention and control of feed source vomitoxin harm.

The invention belongs to the technical field of deep processing of agricultural and sideline products, and particularly relates to an extraction method of wood frog line oil. The extraction method comprises the following steps: putting female wood frogs into water for gut cleaning treatment, and then putting the female wood frogs into warm water for blood discharge treatment; taking out the oviduct of the wood frog after the blood discharge treatment, removing impurities on the surface of the oviduct, and then putting the oviduct into water for cleaning; oil is smeared on the surface of the cotton thread, the fallopian tube with water drained is stretched to be straight, the linear fallopian tube is hung on the cotton thread, and the hung and shaped fallopian tube is air-dried; and taking down the dried wood frog line oil from the cotton thread, cooling the wood frog line oil to room temperature, sealing and storing the wood frog line oil, and finally sterilizing and disinfecting the wood frog line oil. According to the invention, blood discharge treatment is carried out before fallopian tube stripping, so that the color and quality of the product are fundamentally improved; by adding the step of sterilization after drying, the hygienic safety of the product is ensured, the shelf life is prolonged, and the standardized production of the wood frog line oil is realized.

The invention relates to a cherry valley duck PLV virus strain and detection and application thereof, and belongs to the technical field of viruses. According to the invention, a Parry's Lagoon virus (PLV) strain PLV-20155 is separated and identified for the first time, the strain is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.46296. The invention also discloses a preparation method of the parry's Lagoon virus PLV-20155. The PLV-20155 virus can significantly reduce the laying rate of the cherry valley ducks, and causes follicle necrosis and oviduct atrophy. In addition, the invention provides a VP5 gene segment of the PLV-20155 virus and a specific primer of the VP5 gene segment, and the VP5 gene segment and the specific primer can be used for rapidly and accurately detecting the virus. Therefore, the blank of the PLV virus in the field of agricultural economic animal pathogenicity research is filled, a scientific solution is provided for the problem of egg laying reduction of the cherry valley ducks, and important economic value and social significance are achieved.

Provided are: a method for increasing the expression of a foreign protein, the method comprising expressing, in a poultry cell, a construct containing a 3' untranslated region of a gene encoding a foreign protein and a gene encoding an oviduct-specific protein, wherein the 3' untranslated region of the gene encoding an oviduct-specific protein is placed on the 3' side of the gene encoding the foreign protein, and the gene encoding the foreign protein contains a stop codon; poultry knocked-in by placing a construct including a 3' untranslated region of a gene encoding a foreign protein and a gene encoding an oviduct-specific protein in an oviduct-specific locus, wherein the 3' untranslated region of the gene encoding the oviduct-specific protein is placed on the 3' side of the gene encoding the foreign protein and the gene encoding the foreign protein contains a stop codon; and eggs laid by the poultry.

The application discloses a kind of mouse in vivo fertilization oviduct semi-mechanical embryo flushing device, about the technical field of experimental animal equipment, including embryo flushing dish and embryo flushing device, the embryo flushing dish is barrel-shaped, the embryo flushing dish is covered with embryo flushing cover above;Increase the movable oviduct placement dish, can be installed detachable, convenient and practical, avoid the waste of embryo when taking out oviduct.The embryo flushing dish increases the constant-temperature heating ring connected with the embryo flushing dish, reduces the influence of embryo by temperature, ensures the quality of embryo;Embryo flushing needle can realize mouse in vivo fertilization oviduct semi-mechanical, with a large number of water storage, quantitative liquid flushing, self-constant-temperature heating and other advantages;Optimize traditional operation, reduce the workload of personnel, while improving the quantity of embryo collection and experimental yield.

The invention provides a construction method of a non-vaginal uterus fallopian tube mouse model, and belongs to the technical field of biology. The invention provides a construction method of a vagina-free uterus fallopian tube mouse model, which comprises the following steps: mating a Wt1 + / -mouse with an Amhr2-Cre tool mouse to obtain Wt1 + / -; the Amhr2-Cre mouse and the flox mouse with the Wt1 gene being conditionally knocked out copulate, the Wt1 gene in the mouse muller tube interstitial cell genome can be specifically knocked out, and the vagina-free uterus fallopian tube mouse model which is stable in phenotype and high in exogenesis rate is obtained. The method disclosed by the invention provides an important bridge for research and development of medicines for treating female genital malformation and treatment of clinical diseases.

The invention relates to the technical field of portable artificial insemination tools, in particular to a portable artificial insemination tool for poultry and a using method.The portable artificial insemination tool for poultry comprises a seminiferous duct, a pushing mechanism is arranged at one end of the seminiferous duct and pushes out seminal fluid in the seminiferous duct, the other end of the seminiferous duct communicates with an L-shaped guide-in pipe, and the other end of the seminiferous duct communicates with an L-shaped guide-in pipe; the other end of the guide-in pipe communicates with a transfer mechanism, a protective shell is connected to the transfer mechanism, and a supporting handle is installed at the bottom of the protective shell. A connecting piece is arranged at one end of the transfer mechanism and one end of the protective shell; when the device is used and insemination is carried out on hens, seminal fluid is finally output to the fallopian tubes of the hens through the insemination contact tubes, after insemination of each hen is achieved, when insemination is carried out on the next hen, the transfer mechanism, the connecting piece and the tube supply mechanism are matched with one another, and the effect of replacing the insemination contact tubes is achieved; therefore, the problem that cross infection exists in the semen deposition process of hens is effectively solved.