15 results about "Oviduct" patented technology

The application relates to the technical field of traditional Chinese medicine preparation, and particularly discloses a compound traditional Chinese medicine preparation for targeting egg duck and goose egg cytoplasmic disease, which comprises the following components: white head, Coptis chinensis, Huangqi, Phellodendron amurense, wine Danpi, bran-fried peach kernel, Chinese perilla, pomegranate peel, liquorice, honeysuckle, Andrographis, figwort, emblic, ginger, fried atractylodes, fried safflower, Xianhecao, spiny amaranth, and Poria cocos. Through the combination of monarch, minister, assistant and messenger, the core effects of heat-clearing and detoxifying, blood-cooling and dysentery-stopping, and dampness-drying and insect-killing of the medicines are synergistically exerted, the pathogenesis characteristics of the egg cytoplasmic disease are accurately targeted, blood stasis and blood circulation are promoted to eliminate the swelling of the lesion, and the intestinal mucosa damage is repaired to stop bleeding, the symptoms of hematochezia, diarrhea and oviduct inflammation of the egg duck and goose caused by the egg cytoplasmic disease are effectively relieved, the problems of the decreased antibiotic effect, drug residue, bacterial flora disorder and food safety risk are effectively solved, and the compound traditional Chinese medicine preparation has the remarkable progress of drug resistance, safety, no residue, intestinal protection and green environmental protection.

The application provides a monoclonal antibody of a Chlamydia trachomatis Pgp3 protein and application thereof, and the monoclonal antibody comprises a heavy chain variable region CDR1, a heavy chain variable region CDR2 and a heavy chain variable region CDR3 with the amino acid sequences shown in SEQ ID NO. 1, 2 and 3 respectively, and a light chain variable region CDR1, a light chain variable region CDR2 and a light chain variable region CDR3 with the amino acid sequences shown in SEQ ID NO. 4, 5 and 6 respectively. Experimental researches prove that the monoclonal antibody of the Chlamydia trachomatis Pgp3 protein can effectively inhibit infection of Chlamydia trachomatis in a mouse genital tract, and reduce occurrence of oviduct edema.

This invention belongs to the field of agricultural biotechnology, specifically relating to locusts. LARP4B Genes, their dsRNAs, and applications. To provide novel target genes for green pest control, this invention utilizes bioinformatics methods to obtain genes from the transcriptome of locusts. LAPR4B The gene fragment was further cloned and sequenced, yielding the sequence SEQ ID NO: 1. Based on SEQ ID NO: 1, the dsRNA of this gene was designed and synthesized. The nucleotide sequence of this gene fragment is shown in SEQ ID NO: 2. Injecting it into the locust's body cavity specifically silences the target gene, inhibiting the development of the locust's ovary, resulting in a smaller ovary and shorter, narrower oviducts, thereby effectively suppressing the locust population. Due to the specificity of this invention and its effect on ovarian development, it has significant practical implications for suppressing locust populations and can provide a new approach for pest control.

The present application relates to the field of animal breeding technology, in particular to the application of ursolic acid in the frozen semen preservation of sheep. The present application applies ursolic acid in the frozen semen preservation of sheep. The addition of 0.5-2.0 micromole / L ursolic acid in the frozen diluent can improve the quality of frozen semen preservation of sheep and goats, and can improve the sperm motility and vitality after thawing, which helps the thawed sperm to pass through the vagina, uterus and enter the oviduct to complete fertilization, improves the insemination success rate of frozen sperm, and provides technical support for strengthening the protection of local sheep breed resources.

The invention relates to the technical field of veterinary fallopian tube production, in particular to veterinary fallopian tube tail plug assembling equipment which comprises a supporting frame, a vibration feeding disc, a linear vibrator, a mounting plate, a conveying disc and a top plate, the vibration feeding disc, the linear vibrator and the mounting plate are mounted at the top of the supporting frame, and the conveying disc is mounted at the top of the linear vibrator; a fixing plate and a collecting box are fixed to the top of the mounting plate, a top plate is fixed to the top of the mounting plate through a supporting rod, an extending plate is fixed to the top of the top plate, a straight supporting plate is mounted at the top of the mounting plate, a horizontal plate is mounted at the top of the straight supporting plate, and a plurality of butt joint blocks are fixed to the top of the horizontal plate. The overturning motor drives the rotating plate to rotate by 90 degrees, the ten eighth air cylinders on the rotating plate drive the ten sets of clamping clamps to clamp the lock catches correspondingly, the overturning motor drives the clamping clamps and the lock catches to overturn by 90 degrees, the lock catches are overturned to be horizontal, overturning work of the ten lock catches can be completed synchronously, and the assembling machine has the advantage of being high in assembling efficiency.

Provided is a method for constructing a humanized FKBP8 gene knock-in animal model, comprising: (1) constructing a targeting vector for humanized FKBP8 gene knock-in, and transfecting the targeting vector, Cas9, and sgRNA into fertilized eggs of donor animals; (2) transplanting surviving fertilized egg cells into the oviduct of pseudopregnant animals to obtain F0 generation animals and identifying the F0 generation animals, wherein animals showing positive homologous recombination are chimeric animals; and (3) mating the chimeric animals with wild-type animals to obtain F1 generation animals and performing identification, wherein F1 animals showing positive homologous recombination are humanized FKBP8 knock-in animals. In the construction method, an FKBP8 gene sequence in a donor animal is substituted by a human FKBP8 gene sequence, so as to achieve the purpose of humanizing the FKBP8 gene in the donor animal, thus successfully establishing a humanized FKBP8 gene knock-in animal model, so that screening for drugs targeting the FKBP8 gene and a product thereof in the animal model, or studying the pathogenesis and treatment mechanisms of the FKBP8 gene and the product thereof in related clinical diseases can be realized.

The application provides a pharmaceutical composition for treating oviduct inflammation and peritonitis of laying hens and a preparation method thereof, and belongs to the technical field of veterinary drugs. The pharmaceutical composition is obtained by mixing 2-10 parts of artemisinic acid, 1-10 parts of marrubiin hydrochloride, 1-10 parts of sodium salicylate, 0.001-0.005 parts of imipenem / cilastatin sodium and auxiliary materials. The pharmaceutical composition can treat oviduct inflammation, peritonitis and intestinal secondary infection of laying hens through anti-pathogenic microorganism infection and anti-inflammatory pathways, and significantly improves the production performance of laying hens. After the chickens are medicated, the effect is fast, the egg production rate is obviously increased, and the substandard egg rate is obviously decreased. The oviduct inflammation and peritonitis of laying hens over 300 days old can be effectively solved, the clinical use is safe and reliable, the meat and egg food quality and safety can be improved, and it is particularly important for large-scale laying hen farms.

The application discloses a culture solution for improving the blastocyst rate and quality of bovine embryos, application and an in-vitro culture method, and relates to the technical field of animal embryo engineering. The application provides a culture solution for in-vitro culture of bovine embryos, which comprises a first culture solution and a second culture solution with different osmotic pressure concentrations. By controlling the osmotic pressure concentrations of the first culture solution and the second culture solution in a suitable range, and by using the first culture solution in the early stage of embryo development and the second culture solution in the late stage of embryo development, the in-vitro culture system can better simulate the environment in the oviduct in the early stage of embryo development and better simulate the environment in the uterus in the late stage of embryo development, so that the osmotic pressure stress of the embryo in the development process is reduced, and the blastocyst rate and the blastocyst quality of the bovine embryo are improved. Compared with a traditional culture system with constant osmotic pressure, the application significantly improves the blastocyst rate by sequential regulation of the osmotic pressure, the total cell number of the obtained blastocysts is more, and the apoptosis index is lower.

The application relates to the technical field of animal model construction, and particularly discloses a Sypl1 gene knockout colorectal cancer mouse model construction method and application, which comprises the following steps: synthesizing a specific target site gRNA of a Sypl1 gene, mixing Cas9 protein and the target site gRNA to obtain an injection compound, microinjecting the injection compound into mouse zygotes, and transplanting the surviving zygotes into the oviducts of pseudopregnant female mice to obtain F0 generation mice, and the mice born after 20 days are Sypl1 gene knockout mouse animal models; and a chemical induction modeling method is used to construct a colorectal cancer model in wild type mice and gene knockout mice. The Sypl1 gene knockout mouse is constructed by using a CRISPR / Cas mediated genome engineering technology Sypl1 combined with the most widely used colorectal cancer chemical inducer azoxymethane / dextran sodium sulfate, a new colorectal cancer mouse model is constructed, compared with a traditional chemical induction model, the tumor formation rate can be significantly improved, and the tumor formation time can be shortened, and a more efficient animal model is provided for colorectal cancer research.

The invention discloses a method for rapidly constructing a Foxn1 homozygous nude mouse through RepSox induction based on CRISPR / Cas9, and the method comprises the following steps: synthesizing two sgRNAs for respectively identifying a 5'terminal target site and a 3 'terminal target site, the nucleotide sequence of the sgRNA for identifying the 5' terminal target site is as shown in SEQ ID NO: 1, and the nucleotide sequence of the sgRNA for identifying the 3 'terminal target site is as shown in SEQ ID NO: 2; a mixed solution of the two sgRNAs, the Cas protein and the RepSox is microinjected into the fertilized eggs of the rats; the injected embryos are subjected to transient in-vitro culture, survival embryos are screened and transplanted into fallopian tubes or uteruses of pseudopregnant female rats for development, and F0-generation Foxn1 knockout homozygous rats are obtained. According to the method, the Foxn1 gene can be accurately knocked out, the survival rate of a gene editing embryo is improved, the model construction period is shortened, and the rat knockout efficiency is remarkably improved.

The invention provides a feed additive for preventing and controlling poultry salpingitis as well as a preparation method and application of the feed additive, and relates to the technical field of poultry feed additives. The feed additive provided by the invention is prepared by taking a plant source functional component, a prebiotic component, a polyphenol-essential trace element chelate, an essential amino acid balance component, a vitamin and antioxidant component and a high-viable-count probiotic freeze-dried powder component as core components through scientific proportioning and a vacuum low-temperature drying technology. Through cooperation of the multiple components, the poultry intestinal micro-ecological balance is systematically adjusted, and secondary salpingitis caused by the'intestinal tract-reproductive axis' way is inhibited from the source; when the feed additive is applied, 0.5%-6% (preferably 3%-5%) of the feed additive is added into basic ration, and animal experiments show that the feed additive can effectively reduce the number of pathogenic enterobacteria and ammonia nitrogen emission, significantly reduce the occurrence rate (Plt, 0.001) of salpingitis, significantly improve the laying rate and the quality of egg products, and achieve the breeding goals of efficient resistance replacement, safety and environmental protection.

A system for treating a female patient to promote pregnancy comprising a restriction device adapted to postoperatively restrict and release an oviduct of the patient.

The present application relates to the technical field of exosome, and particularly discloses a stable production method of exosome for promoting bovine embryo development and application thereof. The exosome is prepared by a bovine endometrial epithelial cell line or a bovine oviduct epithelial cell, and stable, large and pure exosome can be obtained, which can be used for large-scale production. Meanwhile, the addition of 100 ug / ml of exosome into the culture medium at the 16-cell stage of bovine embryo development can significantly improve the blastocyst rate and blastocyst quality, and provide support for in vitro culture of embryos.

The invention belongs to the technical field of deep processing of agricultural and sideline products, and particularly relates to an extraction method of wood frog line oil. The extraction method comprises the following steps: putting female wood frogs into water for gut cleaning treatment, and then putting the female wood frogs into warm water for blood discharge treatment; taking out the oviduct of the wood frog after the blood discharge treatment, removing impurities on the surface of the oviduct, and then putting the oviduct into water for cleaning; oil is smeared on the surface of the cotton thread, the fallopian tube with water drained is stretched to be straight, the linear fallopian tube is hung on the cotton thread, and the hung and shaped fallopian tube is air-dried; and taking down the dried wood frog line oil from the cotton thread, cooling the wood frog line oil to room temperature, sealing and storing the wood frog line oil, and finally sterilizing and disinfecting the wood frog line oil. According to the invention, blood discharge treatment is carried out before fallopian tube stripping, so that the color and quality of the product are fundamentally improved; by adding the step of sterilization after drying, the hygienic safety of the product is ensured, the shelf life is prolonged, and the standardized production of the wood frog line oil is realized.

The application discloses a kind of mouse in vivo fertilization oviduct semi-mechanical embryo flushing device, about the technical field of experimental animal equipment, including embryo flushing dish and embryo flushing device, the embryo flushing dish is barrel-shaped, the embryo flushing dish is covered with embryo flushing cover above;Increase the movable oviduct placement dish, can be installed detachable, convenient and practical, avoid the waste of embryo when taking out oviduct.The embryo flushing dish increases the constant-temperature heating ring connected with the embryo flushing dish, reduces the influence of embryo by temperature, ensures the quality of embryo;Embryo flushing needle can realize mouse in vivo fertilization oviduct semi-mechanical, with a large number of water storage, quantitative liquid flushing, self-constant-temperature heating and other advantages;Optimize traditional operation, reduce the workload of personnel, while improving the quantity of embryo collection and experimental yield.