The invention discloses a method for improving the application efficiency of frozen
semen of sheep, and relates to the field of animal
reproduction and breeding science, and the method comprises the following steps: collecting
semen, freezing half of the
semen, then respectively carrying out
exosome extraction and identification experiments, then carrying out pandorah sequencing on small non-coding
RNA (Ribonucleic Acid), and finally obtaining the application efficiency of the frozen semen of the sheep. The small non-coding
RNA in
fresh semen and frozen semen is qualitatively and quantitatively analyzed,
differential expression microRNAs are screened out, corresponding target genes and
signal channels are analyzed, and the small non-coding
RNA and the
signal channels related to semen
cryopreservation are screened out. Finally, the differentially expressed small non-coding RNAs are added into frozen semen in a manner of in-vitro
chemical synthesis and the like, or are added in an auxiliary manner during in-vitro
artificial insemination, or are delivered through lipidosome, so that the
phenotype of an
embryo after
insemination is influenced, the conception rate of in-vitro
artificial insemination is increased, the problem in practical production is favorably solved, and the economic benefit is increased. And economic values are created for enterprises.