559 results about "Insemination" patented technology

A method for breeding, especially a method for breeding dairy cattle without use of heat detection prior to insemination.

Improved insemination systems particularly adapted to use for sex-selected sperm sorting include systems which achieve superovulation and then multiple embryo production with sexed embryos. These systems combine with other techniques, including techniques for enhanced sheath fluid and other strategies which minimize stress on the sperm cells, and, potentially, a 2.9 percent sodium citrate sheath solution for bovine species and a hepes bovine gamete media for equine species. Improved collection systems and techniques for the process are described so that commercial application of sperms samples as well as the resulting animals may be achieved.

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comrprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

The invention provides an egg embryo classification based on deep learning. The method comprises the following steps of: collecting embryo images of five days, and dividing the embryo images into three classes of samples including a normal embryo, a suspension embryo and a non-insemination embryo; preprocessing the embryo images, extracting the ROI (Region Of Interest) of each image, and carrying out normalization on the sizes of the images; combining with a transfer learning method, using a CNN (Convolutional Neural Network) model pre-trained by an AlexNet classical network in ImageNet to carry out fine tuning training on a target set; and utilizing the trained model to distinguish an image to be detected. Compared with the prior art, the egg embryo classification is characterized in that the problems of the multiple classifications of the CNN model on an aspect of a small-scale egg embryo data set can be successfully solved, the egg embryo classification is high in accuracy, and the engineering requirements of the detection and the classification of egg embryo survival can be met.

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

The invention relates to a totally artificial breeding method for Coilia ectenes, belonging to the technical field of fish culture. The method is characterized by comprising the following process steps of parent fish source; parent fish select breeding comprising standing / flowing water combined reinforced breeding; artificial induced spawning measure comprising parent fish selection, semen collection, dug roe inspection, induced spawning water temperature confirmation, parent fish matching, hormone injection, artificial insemination and the like; microflow water incubating; and summer flower and fish fry breeding. The invention promotes the parent fish sex gland to grow to maturity and ovulate by measures of reinforcing parent fish nutrition reinforced breeding, feeding hormone, flowing water stimulating and regulating and the like and normalizes the artificial induced spawning and insemination process, originally creates a simple flowing water incubating device of a pelagic egg and reinforces the management and ecological regulation of incubation period, thereby effectively solving the technical problems of artificially breeding Coilia ectenes. The induced spawning rate of artificial breeding is 33 percent, the fertilization rate is 48 percent and the hatching rate is 44 percent.

The invention relates to an apparatus and a method useful for non-surgical embryo transfer or artifical insemination of mammals. An apparatus for depositing media into the uterus of a mammal includes a conical chamber that has a plurality of perforations and flaps. An exterior spiral formation is configured for traversing or penetrating cervical passageway. A sheath having a frusto-conical rearward end extends axially from an aft end of the conical chamber. A tubular depositing chamber extends axially from the conical chamber to a position beyond a fore end of the conical chamber. Coupled to the rearward end of the depositing chamber is an embryo or semen packaging unit. The tubular depositing chamber has an end that has an aperture to permit the flow of semen out of the depositing chamber and into the uterus. The invention also involves a method for depositing of embryos or semen into a uterus of a mammal by a) inserting a conical chamber having a fore end and an exterior spiral formation into a cervix of a mammal, b) securing the conical chamber with the walls of the cervix, c) projecting a depositing chamber through an interior portion of the conical chamber, d) moving the depositing chamber to an embryo or semen release position for release of embryos or semen in the uterus, and e) securing an embryo or semen packaging unit to the depositing chamber to deposit embryos or semen in the uterus.

A sperm cell process system providing to generate sperm cell insemination samples having controlled sperm cell fertility characteristics of sperm cells.

The invention relates to an artificial intensive incubation method for cyprinus carpio koi fries. The method comprises the following steps of: injecting 1 to 3mg of oxytocic hormone domperidone and 2 to 5mu g of chorionic gonadotropin into every 1kg of parent fish of cyprinus carpio koi; filling water into a hybridization pool; putting a mixture of collected roe and semen into 1 to 3 percent physiological saline for insemination by an extrusion method; adhering oosperm to an artificial egg collector by using a brush and putting into an incubator in the hybridization pool for incubation, wherein the water temperature of the hybridization pool is 18 to 23 DEG C; treating by using 18 to 24 ppm saline solution for 15 to 30 minutes; and incubating the roe at the water temperature of between 15 and 25 DEG C for 5 to 7 days for membrane breaking and fry emergence. The method has the obvious advantages of improving the disease resistance of the fries, increasing the incubating rate and survival rate and forming the cyprinus carpio koi fry production technology of intensification, industrialization, mass production and high quality.

The invention discloses a cultivation method of a novel strain of crassostrea gigas with a pure purple left shells. The cultivation method is characterized in that operation comprises the steps of: (1) seed production: selecting male and female individuals with pure purple left shells from a cultured group as cultivating parent shellfishes; performing one-to-one insemination and copulation, respectively marking breeding systems, cultivating larva by adopting strict isolation measures, and placing in a same sea area for intermediate culture and cultivation; breeding generation by generation to purify so as to form a family; and (2) purification of the novel strain: selecting inseparate families with character proportion of above 90% as parent shellfishes, performing seedling propagation so as to cultivate the novel strain of crassostrea gigas with the pure purple left shells and stable character. With the adoption of the cultivation method provided by the invention, the cultivating process of the novel strain is accelerated and transgenic and pharmaceutical treatment do not need to be carried out, the novel strain is cultivated, the additional value of product is also improved through color character, and the market sale price and income of cultivation enterprises and cultivators are increased, and market prospect is wide.

Methods and compositions for synchronizing the time of insemination in swine are described. More particularly, methods are described for synchronizing the time of insemination by administration of a composition comprising a hormone, wherein the swine is inseminated only one time after administration of the hormone, and wherein there is no heat detection.

A method for breeding, especially a method for breeding dairy cattle without use of heat detection prior to insemination.

A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and / or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and / or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.

The invention discloses an antifreeze used for freezing and preserving boar semens as well as preparation and application thereof. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, contains pigeon egg yolk and concretely comprises the following components in parts by volume: 82 to 90 of freezing base liquid and 10 to 18 of pigeon egg yolk. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, has simple preparation method, easy material acquirement, reliable effect and easy popularization, is suitable for insemination after long-time preservation and long-distance transportation after the artificial insemination of the frozen semens of the tubules of boars and the ultra-low temperature freezing of the semens of the boars, can obviously enhance the survival rate of the semens, the integrity of the acrosomes and the integrity of the plasmalemma after the boar semens are frozen and unfrozen, and keeps the insemination activity of the boar semens.

The curled-lip mandarin fish and spotted mandarin fish hybridizing propagation process includes the following steps: 1. reinforced culturing curled-lip mandarin fish as female parent fish and spotted mandarin fish as male parent fish; 2. injecting exogenous hormone to promote mature and induce spawning, flowing water stimulating to promote spawning, artificial dry insemination and managing during hatching, so as to reach spawning rate over 80 %, fertility rate up to 83.5 % and hatching rate up to 89 %; and 3. staged culturing of the hybrid mandarin fish and feeding habit conversion from live fish to non-live feed, so as to reach survival rate of hybrid mandarin fish fry up to 60 %. The hybrid mandarin fish has good taste, high disease resistance, high growth speed and capacity of ingesting non-live fish feed.

The invention discloses technology for culturing a unisexual hybrid scallop by utilizing a purple scallop and a bay scallop, which relates to shellfish breeding technology for aquiculture. The technology comprises the steps of: utilizing two congeneric hermaphroditic scallops with same chromosome number; ripening the two scallops respectively to achieve the synchronous maturity; and then synchronously inducing the two scallops to spawn and spermiate; and acquiring sperms and ovum of the two scallops respectively for interspecies cross-insemination to culture the unisexual hybrid scallop. Obtained offsprings have separated sexes, have males and females, and also have hermaphroditic individuals; ovum from female individuals in the hybrid offsprings can be inseminated by any parental sperm and normally grow; and the hermaphroditic individuals in the hybrid offsprings can be used for quickly purifying a strain.

This invention is a method of separating mammal XY sperm. Fluorescence colorant Hoechst33342 is used to color sperm DNA and then they are separated by flow cytometry according to theory of DNA content of X sperm is more than Y sperm of mammal. Preservation method, sperm diluent, sheath liquor and acceptance liquor used for separating sperm before sperm separating is improved to confirm improve the separation accuracy rate and activity after separation at the same time of ensuring separating XY sperm efficiency. It is fit for big scale commercialization separating generation of XY sperm of scalper, buffalo, goat and other mammal. It can be bonded to artificial insemination and external insemination and other animal breeding new techniques, it greatly improved the efficiency of animal husbandry production.

In the preferred embodiment, bovine semen is mixed with a “suitable solution” to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.

The invention discloses a breeding method for a new strain of crassostrea gigas with orange left and right shells. The breeding method comprises the following steps of selecting individuals with purple left shells and black palliums from a cultured population to be used as male reproduction brood stocks; selecting individuals with black left shells and black palliums to be used as female reproduction brood stocks; carrying out monogamous insemination to establish an F1 family; selecting female and male individuals with black left and right shells and black palliums from the F1 to be used as reproduction brood stocks and establishing an F2 family; continuously selecting female and male individuals with purple black left and right shells and black palliums from the F2 to be used as reproduction brood stocks and establishing an F3 family; selecting orange individuals as parents of the new strain from the F3 and propagating offspring seeds to culture the new strain of the crassostrea gigas with the orange left and right shells and stable characters. The additional value of products is improved and the market prospect is broad.

The invention discloses a new technology for producing frozen mixed semen for controlling the gender of milk cow. Goat semen is used as foundation (2,000,000 to 3,000,000 pieces / 0.25 ml) and is added with X / Y separation semen (500,000 to 1,000,000 pieces / 0.25 ml) to produce the frozen semen for controlling the gender X / Y of the milk cow. The main content of the technology comprises the separation of milk cow X / Y sperms, the preparation of the basic goat semen (which can be substituted by the semen of pig, horse or deer with the equal number) and the production of the frozen semen for controlling the gender of the milk cow through the procedures of mixing and freezing two kinds of the semen according to requirements. The new technology can improve the production efficiency of the frozen semen for controlling the gender of the milk cow by 2 to 4 times and reduce the production cost by 50 to 70 percent simultaneously. The new technology improves the activity and the acrosome intact rate of sperms after the sperms are unfrozen, prolongs the time for having the insemination capacity after the frozen sperms are unfrozen so that the average pregnancy rate in a sexual period reaches over 50 percent; and the accuracy rate of gender control reaches over 90 percent. The new technology is also suitable for producing the frozen mixed semen for controlling the gender of beef cattle.

An artificial insemination (AI) device for the insemination of bovines and other animals including three hollow and essentially cylindrical tubular elements. Each element is provided with an open proximal and an open distal end, the three elements being: an outer protective sheath, an inner sheath, and a semen tube, whereby the diameter of the elements is such that the inner sheath is axially movable in the outer protective sheath and the semen tube is axially movable in the inner sheath, whereby the length of the outer sheath is less than the length of the inner sheath and whereby the inner sheath and the semen tube are jointly axially displaceable in and through the outer sheath. An outer sheath, an inner sheath and a semen tube for use in an AI device and a method for the insemination of bovines and other animals, includes the steps of inserting through the cervix and into the uterine horn, a closed tubular element to a point near the major curve of the horn, opening the tubular element by protrusion of an inner tubular element and manually guiding it into the curve of the horn and positioning the distal end of the inner tubular element near the ovary, and further providing semen nearby the UTJ through an internal semen channel.

The invention relates to a digital behavior recognition device for dairy cows, which comprises an ear tag, which is used to bind on the ear of a dairy cow and collect the behavior state data of the dairy cow in real time, a reader-writer used for acquiring the ear tags in a radio frequency manner to acquire behavior state data of the dairy cows and performing model conversion; and a cloud server used for acquiring the data converted by the reader-writer model in a wireless manner and intelligently judging whether the current behavior of the dairy cow is in estrus or not. A behavior data modelis generated by accurately collecting all behavior state data of the dairy cows, whether the dairy cows are in oestrus or not is judged. The optimal insemination time is calculated, the conception rate of the individual dairy cows is increased. The calving interval is shortened, and the method can be widely applied to dairy cow breeding.

An improved animal-use artificial inseminator comprised of an insemination tube with a nozzle at its front extremity into which an insemination sheath is inserted and a connector at the rear extremity of the insemination tube having a check membrane. After the insemination tube penetrates the female animal body vagina and the front end of the nozzle is nominally at the first cervical ring of the cervix, the semen bottle insertion tube is utilized to pierce the connector check membrane at the rear extremity of the insemination tube and when the semen at the insemination tube is squeezed, semen is automatically squeezed out of the insemination sheath. The soft insemination sheath proceeds along the cervix without the occurrence of friction, the front end smoothly moving forward until it reaches the position of the womb. Contractions of the womb are utilized to articulate the insemination sheath such that the contained semen is ejaculated into the two Fallopian tubes or the womb from the slits along the two sides of the front end. At the same time, after the semen bottle is pulled out, since the connector check membrane automatically opens and closes along with the contraction of the womb, not only is the semen in the insemination sheath prevented from backflow or leakage, but air is admitted for smooth and complete squeezing into the Fallopian tubes or the womb.

The present invention includes methods and apparatus for producing and using an improved artificial insemination device that prevents contaminants from entering the opening at the end of an insemination sheath during insertion through the reproductive tract. A thin protective cover or membrane is provided at the tip of the sheath to seal it, the membrane being designed to rupture under the pressure from the seminal fluid being applied by the plunger used to eject the semen from the insemination gun. The protective cover can be an integrated portion of the sheath itself, or a material applied to the tip of the sheath. The protective cover is minimal in size as to not increase the diameter of the artificial insemination device so that there is no loss in tactile sensitivity to the breeder who must maneuver the invention through the reproductive tract.

In the preferred embodiment, bovine semen is mixed with a suitable solution to form an insemination medium which is injected into the reproductive organs of a female bovine to flood the uterine horns in a nonsurgical AI procedure. The invention includes an AI instrument having a disposable, single use pipette that may be used with this nonsurgical procedure. The AI instrument does not include a balloon and is referred to as “catheter free”. Preferably, the uterine horns are flooded concurrently, but in the alternative, they may be flooded sequentially.

The present invention relates to a method for propagation and cultivation of platycerium. It is characterized by that said invention adopts sterile spore germination technique and makes it be combined with test-tube seedling-cultivating technique to implement propagation and cultivation of platycerium. Said method includes the following several steps: collecting storing, health and mature spore, making sterile spore germination, gametophyte growth culture, insemination to form zygoite, sporophyte enrichment culture, rooting culture and transplantation, etc.

The invention relates to an abduction method of a cynoglossus semilaevis diploid fry that is manually induced to be spawned and developed from female nucleus, comprising manually induced spawning of a parent fish, genetic inactivation of sperms, and an induction and identification method of the female nucleus developed diploid fry. The invention firstly establishes a method to obtain large batches of unfertilized ovum by manually induced spawning of the cynoglossus semilaevis, and builds up a method that the cynoglossus semilaevis sperms and lateolabrax japonicus sperms are adopted to induce the developing of the female nucleus of the cynoglossus semilaevis and obtain the female nucleus diploid fry, thus sieving effective manually induced spawning hormones kinds and dosages, and sieving cold shock beginning time and temperature and time that are suitable for the development of the female nucleus of the cynoglossus semilaevis, and the identification method of the female nucleus developed fry of the cynoglossus semilaevis is established. The insemination rate of the ovum of the cynoglossus semilaevis obtained by the method is 60 to 89 percent, the hatch ability is 34 to 79 percent, and the induction rate of the female nucleus developed fry is 0.4 to 6.3 percent. The invention is characterized by advancement, high effect, practicability and reliability, and has important application value and wide popularization prospect on the breeding of fry kinds of the cynoglossus semilaevis, sex control and hologynic breeding.

The invention relates to a method of producing a protein of interest, comprising making a non-human transgenic mammal that produces said protein in its milk, obtaining said milk from the non-human transgenic mammal and purifying said protein of interest from the milk. Transgenic bovine animals were generated, which are able to produce human growth hormone in mammary glands. The method involves cloning of a genetic construct encoding hGH gene and beta casein promoter conveniently in an expression vector. It also includes transfection procedures into fetal bovine somatic cells, generally fibroblasts, and the nuclear transfer into enucleated bovine oocytes, generating thus transgenic embryos. The method also includes other procedures to generate transgenic embryos for the further expansion of the transgenic herd, such as the subcloning of transgenic female bovines, the superovulation of transgenic cows and their insemination with semen from a non-transgenic or a transgenic male bovine, and the superovulation of non-transgenic cows and their insemination with semen from a transgenic male bovine. Afterwards, transgenic embryos give rise to transgenic cattle that produce human growth hormone in huge amounts in their milk, from which the hormone is completely purified and analysed to fulfill all the requirements for the manufacture of a pure biopharmaceutical product.