61 results about "Bumblebee" patented technology

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention discloses a special feed for breeding bumblebee pollination swarms and a preparation method thereof, and belongs to the technical field of insect feed preparation, and the special feed for breeding the bumblebee pollination swarms comprises the following raw materials: white granulated sugar, glucose, potassium sorbate, epsilon-polylysine, vitamins, trace elements and water; the preparation method comprises the following steps: adding the white granulated sugar and the glucose into boiling water, uniformly stirring, cooling, adding the vitamins, the trace elements, the potassium sorbate and the epsilon-polylysine, uniformly stirring, and cooling to room temperature. The components such as the sugar source, the vitamins and the preservative are introduced to achieve a synergistic effect, so that the freshness of the sugar water is guaranteed, the intake of nutrient substances by the bee colony is improved, the survival rate of the bee colony is effectively increased, the development time of the early-stage bear bee colony is shortened, the growth speed of the early-stage pollination bee colony is accelerated, and the colony potential of the bee colony before pollination is increased.

The invention relates to an eDNA method for identifying bumblebee honeycombs in permafrost regions of Qinghai-Tibet Plateau. The eDNA method comprises the following steps: S1, positioning observation sample belts and arranging sampling points on the basis of multi-source data in combination with a Bayesian hierarchical model; s2, performing deep freezing and complete excavation on matrixes around and inside all the sampling points; s3, stratified sampling and anti-degradation treatment are carried out, and a spatial attribute database is established; s4, extracting and purifying eDNA (enhanced deoxyribonucleic acid); s5, determining a nest host based on PCR amplification, high-throughput sequencing and sequence comparative analysis; and S6, constructing a Bayesian hierarchical model in combination with biological information, and judging honeycomb characteristics. The method is simple, rapid and low in cost, not only is suitable for Qinghai-Tibet Plateau permafrost regions, has the core advantages of non-destructiveness and high sensitivity, but also is also suitable for underground insect nest identification research in other environment sensitive regions or regions difficult to physically explore in principle, and has wide application prospects. The method is especially suitable for monitoring rare, secret or difficult-to-approach organisms.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

A technology for relieving diapause of bumblebee queen bees is realized mainly by performing worker bee induction at key nodes of bumblebee queen breeding activation: after mating bumblebee queen bees are activated, selecting a worker bee which just comes out of a house for one day from a bee supply colony to join a queen bee feeding group, inducing the queen bees to relieve diapause, and entering a breeding state; after 5-7 days, replacing the worker bees added before with new worker bees to prevent the worker bees from laying eggs; the process is repeated for two to three times until the queen bees lay eggs; through worker bee induction, bumblebee queen diapause can be quickly relieved, the queen bee is stimulated to lay eggs in advance, and the breeding period can be shortened by about three months; the bumblebee queen is not subjected to low-temperature diapause, so that the vigorous vitality of the bumblebee queen is kept, the breeding efficiency of the bumblebee queen is improved, and the seed selection and breeding process is accelerated.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

1. Name of the product in this design: Drone Remote Controller (Little Bumblebee Series). 2. Purpose of this design: For use as a drone remote controller. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of microorganisms, in particular to bumblebee Starmomyces sp. And application of the bumblebee Starmomyces sp. In preparation of glycolipid with anti-aging and / or moisturizing functions. The invention provides bumblebee raw Starmonia yeast with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2025061, and provides a preparation method of glycolipid. When being used for preparing glycolipid, the strain has the advantages of short fermentation time, high grease utilization rate and high glycolipid yield. Moreover, the glycolipid obtained by the method is a mixed glycolipid of sophorolipid, glycerol glycolipid and glucose lipid, and can be widely applied to the fields of medicines, foods, agriculture, environmental protection, cosmetics and the like, and the production cost of the glycolipid can be greatly reduced.

The present invention relates to the field of bumblebee handling in particular in relation to bumblebee rearing, storage, transport and at the pollination location. For these purposes the invention according to a first aspect provides a housing, such as a nest box, for bumblebee (Bombus spp.) individuals, including bumblebee workers. Further aspects of the invention relate to the use of the housing for confining bumblebees and a method of pollinating blooming plants comprising providing in the vicinity of the plants a number of bumblebee housings according to the invention.

The invention discloses a probiotic composition for regulating intestinal health of bumblebees and application of the probiotic composition. The probiotic composition is prepared from the following raw materials in percentage by mass: 1-2% of bacillus subtilis, 20-30% of sucrose water, 5-8% of yeast powder, 1-2% of honey, 25-40% of apricot pollen, 0.05% of mixed vitamins, 0.05% of a proline solution and the balance of water. According to the probiotic mixture disclosed by the invention, worker bee death caused by escherichia coli infection can be effectively reduced; the feed does not cause the change of the core flora of intestinal microorganisms of bumblebees, namely does not cause the disorder of the intestinal microorganisms, can promote the metabolism of fatty acid, nitrogen metabolism and energy metabolism in intestinal tracts, and is beneficial to the intestinal health of bumblebees.

This application discloses a bumblebee feed for artificial breeding and its preparation method, relating to the field of feed technology. It addresses the problem of unclear dosage of potassium sorbate in bumblebee feed, leading to uncontrollable preservative effects or adverse effects on bumblebees. The feed consists of a feed base and potassium sorbate as a preservative, with a mass percentage of 0.08%–0.12%. The preparation method involves mixing potassium sorbate with the feed base to achieve a final mass percentage of 0.08%–0.12% in the feed. The mixing time is 5–30 minutes, and the mixing temperature is 15–30°C. A mother liquor method is used for sugar water feed, and a premixing method is used for pollen feed. This application experimentally determined a specific safe dosage of 0.1%, which can keep the feed fresh and mold-free for one week under conditions of 28°C and 55% humidity, ensuring safety for bumblebees and providing a directly applicable preservative feed technology solution for commercial bumblebee breeding.

The invention discloses a low-voltage flexible DC micro-grid fault positioning method based on an improved Tiancattle swarm algorithm. According to the method, the fault point can be accurately positioned when the inter-electrode short-circuit fault or the single-electrode grounding fault occurs in the flexible direct-current power distribution network. The method is slightly influenced by excessive resistance, and a fault positioning problem is converted into a fault parameter identification problem. And in combination with the improved Tiancattle swarm algorithm, the accuracy of fault point positioning can be improved, errors are reduced, and the information distortion resistance is improved. The method is of great significance in improving the safety and reliability of the direct-current power distribution system.

The utility model relates to the technical field of bee breeding, in particular to a bumblebee species cultivating device which comprises a box body, lining plates are attached to the inner wall of the box body, a partition plate is connected between every two opposite lining plates and divides the box body into a feed area and a spawning area, the lining plates and the partition plate are both made of wood, and the partition plate is made of wood. A partition plate is arranged in the box body, a through opening for bumblebees to pass through is formed in the partition plate, a ventilation opening is formed in the side, away from the feed area, of the box body, a first sealing cover plate and a second sealing cover plate are arranged on the feed area and the egg laying area in a clamped mode respectively, a water tank is connected into the feed area, and a supply assembly for supplying sweet water at regular time is arranged on the first sealing cover plate. A temperature and humidity control system used for controlling temperature and humidity is arranged in the spawning area, and the inner wall of the spawning area is coated with natural beewax. The device has the effects of improving the matching degree of the breeding device and the nest environment and improving the problem that sweet water is prone to going bad.

The present invention discloses a method for constructing a bumblebee model of acute or chronic neuropathy. The method uses newly emerged bumblebees as the subjects for establishing the bumblebee model of neuropathy; acrylamide is dissolved in a sterile sucrose solution to prepare an acrylamide sucrose solution; the newly emerged bumblebees are fed the acrylamide sucrose solution to obtain the bumblebee model of neuropathy; the model constructed by the method of the present invention can be used to screen methods or drugs for preventing or treating neurotoxicity-related diseases, can be applied to explore the specific mechanisms of nervous system-related diseases, and has good application prospects.

The present application relates to the technical field of fermentation detection, and particularly relates to a camellia oil fermentation detection method of bear-born candida, which comprises the following steps: preparing camellia oil emulsion; mixing the camellia oil emulsion with bear-born candida seed liquid, and feeding gas to obtain fermentation liquid; detecting the stirring shaft torque of the fermentation tank; adjusting the feeding rate of the camellia oil emulsion; detecting the attenuation rate of the volume oxygen transfer coefficient of the fermentation liquid after the feeding rate is adjusted; adjusting the aeration mode of the fermentation liquid, including adjusting the gas-liquid contact time or carrying out pulse aeration; continuously monitoring the oil-water liquid level potential of the fermentation liquid after the aeration mode is adjusted; determining whether the fermentation liquid reaches the fermentation end point according to the change curve of the oil-water liquid level potential; if the fermentation liquid reaches the fermentation end point, determining the stirring speed of the stirring shaft; and obtaining the bear-born candida after fermentation. The present application realizes the improvement of the accuracy of fermentation process control.

The invention relates to the technical field of microbial fermentation, in particular to a preparation method and application of microbial fermentation nano-emulsion. Through a microbial fermentation method, a stable cosmetic microbial nano-emulsion raw material which is free of an exogenous surfactant and has remarkable moisturizing and soothing effects is provided, and the microbial fermentation nano-emulsion raw material comprises water, grease, saccharomyces cerevisiae and a second strain, and the second strain is selected from at least one of steda oil saccharomycetes, bumblebee candida and rhodosporidium. An emulsion system based on the prepared nano-emulsion is relatively small in particle size, good in stability, relatively high in oxidation resistance and excellent in moisturizing effect, and the raw materials are safe and stable, so that the nano-emulsion has a relatively great market application prospect.

The utility model discloses a bear bee breeds and experimental observation integrated multifunctional platform, including the experimental layer and the breeding layer that arrange from top to bottom in proper order, the inside of this experimental layer is equipped with mechanical arm and the hunger closed space that connects the conduction in proper order, experimental closed space and collection closed space, this hunger closed space and experimental closed space conduction place are equipped with the first lifting plate that can realize the opening and closing through the lifting, this experimental closed space and collection closed space conduction place are equipped with the second lifting plate that can realize the opening and closing through the lifting, this mechanical arm is used for controlling the lifting opening and closing of first lifting plate and second lifting plate, the inside of this breeding layer is equipped with lifting conveyor, and the breeding closed space and excretion closed space that are connected with each other and are communicated, and this lifting conveyor connects breeding closed space and hunger closed space, and the lifting conveyor is used for sending bear peak from breeding closed space to hunger closed space, and this scheme can solve the problem of single function of the existing bear peak breeding box.

The invention discloses a miniature intelligent simulation beehive system special for bumblebees, and relates to the field of intelligent control. A bumblebee inlet and outlet and an observation window are formed in the front face of a main body beehive body in the system; the environmental parameter adjusting unit is arranged in the main body box body and is connected with the intelligent control unit; the environmental parameter adjusting unit collects environmental parameters in the main box body; the field adaptation unit comprises a solar power supply module arranged at the top of the main body box body, a storage battery connected with the solar power supply module, and a protection device and an environment adaptation module which are arranged outside the main body box body; the auxiliary unit performs data recording and remote monitoring; the intelligent control unit is used for storing preset parameters and controlling the environmental parameter adjusting unit and the electrochromic glass light transmittance of the observation window according to the environmental parameters and the preset parameters, so that the environmental parameters in the main box body are in a corresponding preset range; the system can intelligently adjust environmental parameters, and is suitable for indoor experiments and outdoor wild environments.

Selective capture device for Asian hornets (Vespa velutina) with unidirectional mechanical entrances. The invention relates to a device comprising a cell (1) with calibrated outlets (4), a bait container (8) equipped with a protective grid (9), and at least one lid (2) including calibrated outlets (4), characterized in that the capture device (11) includes at least one calibrated inlet (3) equipped with a lever (5) that mechanically blocks the exit of hornets from the inside to the outside of the device (11) through this calibrated inlet (3). The device according to the invention is particularly suitable for the capture of Asian hornet queens both preventively and curatively. Figure for the abstract: Fig 2

The application provides a method for artificially assisting short-headed bumblebee larva rearing, and relates to the technical field of bumblebee rearing. The method for artificially assisting short-headed bumblebee larva rearing is mainly as follows: a small bee group, an egg-producing bee group and a rearing bee group are established, bee eggs with a production time less than 2 hours are collected in the egg-producing bee group, small larvae are obtained after artificial hatching, the artificially hatched larvae are transferred into egg bags of the rearing bee group from which eggs and larvae are removed, and the larvae are reared to adult bees by the rearing bee group. The method provides an egg, egg hatching and larva rearing method for the research on the biological characteristics of the egg structure, embryonic development, genetic variation and gene function of the short-headed bumblebee. The application creates a bumblebee young age artificial rearing technical mode, and provides controllable test materials and a rearing method for the research on the biological characteristics of the bumblebee.

The invention relates to a distributed photovoltaic energy storage grid-connected power dual-objective optimization control method based on a Tiancattle swarm algorithm, and belongs to the technical field of distributed photovoltaic energy storage grid-connected control. Aiming at the problems of voltage out-of-limit and operation economy of the power distribution network caused by superposition of photovoltaic fluctuation and load fluctuation, the method comprises the following steps: establishing a light storage system model, setting a double-layer objective function with the lowest system operation cost and the minimum voltage fluctuation, applying power constraint, and performing optimization solution by adopting a Tiancattle swarm algorithm. The dual optimization objectives of power distribution network node voltage stabilization and system economic operation are achieved, and the photovoltaic absorption capacity and optimization efficiency are effectively improved.

The invention discloses a method for increasing the yield of sophorolipid and recombinant bacteria used by the method. The recombinant strain is an engineering improved strain 2 * CYP which is obtained by over-expressing CYP52M1 protein with an amino acid sequence shown as SEQ ID NO: 2 in candida bumblebee ATCC 22214 capable of producing sophorolipid. Experiments prove that compared with the candida bumblebee ATCC 22214, the sophorolipid content in the fermentation liquor obtained by fermenting the engineering improved strain 2 * CYP is increased by 22.67% and reaches a remarkable level. According to the method, the CYP52M1 protein is expressed by adopting free plasmids in the bumblebee candida ATCC 22214 for the first time, so that the yield of sophorolipid is remarkably increased, and the construction operation difficulty and the experiment cost of recombinant bacteria are simplified. The method has an important application value.

The present application relates to the field of biological medicine, and discloses a fermentation product of lavender flowers and camellia seed oil, and a preparation method and application thereof. The preparation method of the fermentation product of the lavender flowers and the camellia seed oil comprises the following steps: fermenting a flower oil mixture by using a bumblebee s. sternai yeast, and taking an oil phase product after fermentation; the flower oil mixture is a mixture of the lavender flowers and the camellia seed oil. The method can efficiently extract essential oil in the lavender flowers, the mixed product of the lavender flowers and the camellia seed oil after fermentation has a synergistic effect in efficacy, has good anti-inflammatory and moisturizing effects, and can be applied in anti-inflammatory products and moisturizing products.

The invention provides application of insulin in preparation of a medicine for terminating bumblebee diapause, and belongs to the technical field of insect breeding. Insulin is used as a core raw material, a queen European bumblebee 24 hours after mating is selected, carbon dioxide is adopted for slight anesthesia treatment, and then a microsyringe is used for injecting insulin dissolved in a phosphate buffer solution into the third abdominal node and the fourth abdominal node of the belly of the queen bee. Insulin is applied to bumblebee diapause regulation for the first time, queen bee diapause can be quickly and effectively terminated, the artificial feeding and breeding efficiency can be remarkably improved, the survival rate of swarms is increased, the development time of early-stage bear swarms is shortened, the growth speed of pollination swarms is increased, and the swarms potential before pollination is increased; timely and high-quality pollination bee colony support is provided for facility agriculture, and the method has a wide industrial application prospect.