107 results about "Bumblebee" patented technology

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention discloses a method for purifying modified sophorolipid, which comprises the following steps: adding a salt solution into lactone type crude sophorolipid obtained by fermenting candida bumblebee, stirring, standing, and collecting a lower layer sophorolipid phase; according to the method, the produced crude sophorolipid with low or high emulsification degree is purified, impurities are removed, good appearance conditions are achieved, and the appearance requirement of the commodity sophorolipid is met. And meanwhile, the sophorolipid is modified in the purification process by utilizing the chemical properties of different salts, so that the decoloration of the sophorolipid is realized, the concentration of the lactone type sophorolipid is improved, and the stability of the lactone type sophorolipid is improved at the same time.

The invention belongs to the technical field of microbial fermentation, and particularly relates to a fermentation strain composition, fermentation oil as well as a preparation method and application of the fermentation oil. The raw materials of the fermented oil comprise grease, candida bumblebee and a second strain; and the second strain is selected from at least one of lipomyces starkeyi, saccharomyces cerevisiae, rhodosporidium torulosporioides, lucid ganoderma and cordyceps sobolifera. The preparation method comprises the following steps: firstly, adding grease into a culture medium, inoculating bumblebee candida and a second strain, and fermenting; and sterilizing and filtering after fermentation. The invention provides a grease raw material fermentation strain combination capable of improving the grease stability, remarkably reducing the dosage of a grease emulsifier and improving the transdermal absorption effect of grease as an active matter carrier, and provides a basis for the application of the grease raw material fermentation strain combination in cosmetics.

The invention discloses a special feed for breeding bumblebee pollination swarms and a preparation method thereof, and belongs to the technical field of insect feed preparation, and the special feed for breeding the bumblebee pollination swarms comprises the following raw materials: white granulated sugar, glucose, potassium sorbate, epsilon-polylysine, vitamins, trace elements and water; the preparation method comprises the following steps: adding the white granulated sugar and the glucose into boiling water, uniformly stirring, cooling, adding the vitamins, the trace elements, the potassium sorbate and the epsilon-polylysine, uniformly stirring, and cooling to room temperature. The components such as the sugar source, the vitamins and the preservative are introduced to achieve a synergistic effect, so that the freshness of the sugar water is guaranteed, the intake of nutrient substances by the bee colony is improved, the survival rate of the bee colony is effectively increased, the development time of the early-stage bear bee colony is shortened, the growth speed of the early-stage pollination bee colony is accelerated, and the colony potential of the bee colony before pollination is increased.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention provides a preparation method of a hydrolyzed collagen product with surface amphipathicity as well as the product and application thereof, and the preparation method comprises the following steps: (1) taking hydrolyzed collagen enzymatic hydrolysate as a substrate, then preparing a fermentation culture medium with vegetable oil and sugar, and fermenting by adopting candida bumblebee; (2) after fermentation, adding lysozyme into the fermentation liquor for activating treatment, and then sterilizing, centrifuging and filtering; and (3) carrying out freeze-drying treatment on the filtered fermentation liquor to obtain the hydrolyzed collagen product with surface amphipathicity. The hydrolyzed collagen product prepared by the invention not only has low molecular weight and high permeability to skin, but also has water locking and moistening effects on the skin surface due to the amphiphilic sophorolipid on the surface, so that the moisture retention of the skin is better improved, and the product has excellent product characteristics and has wide market prospects.

The invention relates to an eDNA method for identifying bumblebee honeycombs in permafrost regions of Qinghai-Tibet Plateau. The eDNA method comprises the following steps: S1, positioning observation sample belts and arranging sampling points on the basis of multi-source data in combination with a Bayesian hierarchical model; s2, performing deep freezing and complete excavation on matrixes around and inside all the sampling points; s3, stratified sampling and anti-degradation treatment are carried out, and a spatial attribute database is established; s4, extracting and purifying eDNA (enhanced deoxyribonucleic acid); s5, determining a nest host based on PCR amplification, high-throughput sequencing and sequence comparative analysis; and S6, constructing a Bayesian hierarchical model in combination with biological information, and judging honeycomb characteristics. The method is simple, rapid and low in cost, not only is suitable for Qinghai-Tibet Plateau permafrost regions, has the core advantages of non-destructiveness and high sensitivity, but also is also suitable for underground insect nest identification research in other environment sensitive regions or regions difficult to physically explore in principle, and has wide application prospects. The method is especially suitable for monitoring rare, secret or difficult-to-approach organisms.

The invention belongs to the technical field of bumblebee queen breeding, and discloses a small-scale bumblebee queen breeding method which is mainly technically characterized by comprising the following steps: preparing a mother-generation queen bee, inducing the mother-generation queen bee to lay eggs, and breeding offspring queen bees. According to the method for rapidly breeding the bumblebee queen on a small scale, the queen breeding duration is short, and compared with a conventional queen breeding method, the queen breeding duration of the method is shortened by 60 days. The accuracy is high, and the bee colony of bred offspring queen bees is 95-100%. Space is saved, the number of worker bees is small, the honeycomb is small in size, and a large feeding box is not needed.

The invention relates to a method for increasing the content of ceramide in fermented tea-seed oil and application of the ceramide in skin care products, and the method comprises the following steps: taking a mixture containing tea-seed oil and a culture medium, and carrying out sterilization treatment to obtain an initial fermentation system; inoculating bumblebee candida into the initial fermentation system, carrying out fermentation culture until first culture time is reached, and adding a carbon source; continuously carrying out fermentation culture until the final culture time is reached, so as to obtain fermentation liquor; the fermentation broth is subjected to layering, centrifugation, dehydration and deodorization treatment after being inactivated so as to obtain the fermented tea seed oil, and the preservation number of the candida bumblebee is CGMCC No.25797. The fermented tea seed oil prepared by the technical scheme provided by the invention has more excellent barrier repairing and skin stability maintaining functions on human skin cells, and also has more remarkable effects in the aspects of relieving, anti-inflammation, moisturizing, anti-oxidation and the like.

The invention discloses a method for promoting development of swallowing glands of bumblebees, the method is characterized in that the mass ratio of linoleic acid to alpha-linolenic acid in bumblebee feed is adjusted to be (1-5): 1, and the development of the swallowing glands of the bumblebees is promoted, the method is simple and easy to implement, and by adjusting the ratio of the linoleic acid to the alpha-linolenic acid in the bumblebee feed, the development of the swallowing glands of the bumblebees is promoted. According to the method, normal development of the giant bumblebees is guaranteed, diet preference of the giant bumblebees is met, nutrient fluctuation of natural pollen is made up, development of swallow glands can be remarkably promoted, feeding of larvae of the giant bumblebees is facilitated, and growth and development of offspring of a bee colony are facilitated.

The utility model relates to a bumblebee feeding device, and belongs to the technical field of bumblebee feeding. The front side of the beehive body is open, a supporting plate inserting groove and a tray inserting groove are formed in the bottom in the beehive body, the supporting net plate and the tray are detachably inserted into the supporting plate inserting groove and the tray inserting groove respectively, sliding grooves are formed in the two sides of the front portion of the beehive body, the door body is detachably installed in the sliding grooves, and ventilation windows are arranged on the two sides of the beehive body. The sweet water feeder and the pollen feeder are both mounted at the top of the bee box body; two supporting net plates are arranged, one supporting net plate is in a working state, the other supporting net plate is in a standby state, in the breeding process, the standby supporting net plate is inserted into the supporting plate inserting groove to abut against and abut against the supporting net plate in the working state, the standby supporting net plate is slowly pushed in to push out the supporting net plate in the working state, replacement of the supporting net plates is achieved, bumblebees cannot be hurt, and the breeding efficiency is improved. Stimulation to bumblebees is small, and operation is convenient and safe; the detachable supporting net plate and the tray are convenient to detach, clean and disinfect, the clean bumblebee feeding environment is guaranteed, and the health of bumblebees is guaranteed.

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.

The utility model discloses a semi-closed greenhouse beehive device, which belongs to the technical field of semi-closed greenhouse beehives and is mounted below a substrate groove and in front of an air outlet of an air duct. The beehive device comprises a hoisting mechanism, a support and a beehive body. The lifting mechanism comprises a hook and a lifting frame connected with the hook in a hooked mode, the lifting frame is connected with the support, and the beehive body is installed on the support. The beehive device is redesigned, firstly, temperature rise caused by sunlight is reduced through shading of plants and shielding of an upper building, secondly, the ventilation condition of the beehive is improved through air outlet of the air duct, the surrounding environment condition is adjusted, and then the activity degree of bumblebees is guaranteed.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

A technology for relieving diapause of bumblebee queen bees is realized mainly by performing worker bee induction at key nodes of bumblebee queen breeding activation: after mating bumblebee queen bees are activated, selecting a worker bee which just comes out of a house for one day from a bee supply colony to join a queen bee feeding group, inducing the queen bees to relieve diapause, and entering a breeding state; after 5-7 days, replacing the worker bees added before with new worker bees to prevent the worker bees from laying eggs; the process is repeated for two to three times until the queen bees lay eggs; through worker bee induction, bumblebee queen diapause can be quickly relieved, the queen bee is stimulated to lay eggs in advance, and the breeding period can be shortened by about three months; the bumblebee queen is not subjected to low-temperature diapause, so that the vigorous vitality of the bumblebee queen is kept, the breeding efficiency of the bumblebee queen is improved, and the seed selection and breeding process is accelerated.

The present invention discloses a method and device for predicting the fighting behavior of bumblebees. The method comprises: obtaining a training sample set, wherein the training sample set is a plurality of photos of bumblebee colonies marked with label categories; identifying the training samples of the training sample set according to a fighting recognition model, and obtaining a polar coordinate system of the bumblebee outline, wherein the fighting recognition model is a residual convolutional neural network model fused with an attention mechanism in a pyramid structure; determining the training prediction result of the fighting recognition model according to the polar coordinate system, and determining that the fighting recognition model has completed training when the training prediction result reaches a preset target; and identifying bumblebees with fighting behavior according to the fighting recognition model that has completed training. The prediction method uses a primary classification obtained by the fighting recognition model and a secondary classification obtained based on the bumblebee outline to jointly predict the fighting situation of the bumblebees at the location. The prediction method combines the bumblebee picture features and the bumblebee outline features, thereby improving the accuracy of predicting the bumblebee fighting behavior.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

The present invention relates to a breeding box, and in particular to a bumblebee breeding box, comprising a box body, a support plate, a box door, a blocking block and a grid frame, etc., wherein two support plates are connected to the bottom of the box body, the box door is rotatably connected to the side of the box body, an outlet is opened on the box body, a blocking block for blocking the outlet is slidably connected to the box body, and a grid frame for bumblebee breeding is slidably connected to the side of the box body. The present invention can be used for bumblebee breeding through the grid frame, and the feces of the bumblebees will pass through the retaining net and fall onto the collecting belt. When the feces on the collecting belt needs to be cleaned, the second roller can be rotated, and the second roller drives the collecting belt to rotate, and the side of the collecting belt with feces is turned out of the box body, so as to facilitate the cleaning of the feces; water can be sucked into the water outlet pipe through a water pump, and the water is sprayed through a nozzle and sprayed onto the collecting belt, so as to automatically wash the feces on the collecting belt, so as to facilitate the cleaning of the collecting belt.

A trap 1 for catching insects, such as Asian hornets, with a receptacle for receiving an insect attractant. The bait may be liquid and the receptacle may hold a volume of water. There is a closure 3 which may be releasably fastened 12 to the receptacle. The closure has a recessive surface, such as a cone or funnel, which extends into the receptacle, and an aperture 9 for allowing an insect into the receptacle. There are a plurality of fins, walls or partitions 30 on the receptacle, to guide the insect to the aperture. The fins may be upstanding, radially disposed on the funnel, or trapezoidal in shape. There may be bait odour vents on the recessive surface. There may be bycatch holes 10 to release smaller non-target insects. The trap may have feet 11 or an oversized closure so it can be rested horizontally on its side. The aperture may lead to an L shaped hollow elbow internal the receptacle, for reducing escape of the insects. The elbow may be perforated to allow odour through. A method of using the trap near beehives is disclosed. The trap may be immersed in water to exterminate trapped insects.

1. Name of the product in this design: Drone Remote Controller (Little Bumblebee Series). 2. Purpose of this design: For use as a drone remote controller. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of microorganisms, in particular to bumblebee Starmomyces sp. And application of the bumblebee Starmomyces sp. In preparation of glycolipid with anti-aging and / or moisturizing functions. The invention provides bumblebee raw Starmonia yeast with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2025061, and provides a preparation method of glycolipid. When being used for preparing glycolipid, the strain has the advantages of short fermentation time, high grease utilization rate and high glycolipid yield. Moreover, the glycolipid obtained by the method is a mixed glycolipid of sophorolipid, glycerol glycolipid and glucose lipid, and can be widely applied to the fields of medicines, foods, agriculture, environmental protection, cosmetics and the like, and the production cost of the glycolipid can be greatly reduced.

1. The name of the product of this design: packaging bottle (Bee). 2. Purpose of the product of this design: used to hold honey or beverages. 3. The design key point of this appearance design product lies in the combination of shape and pattern. 4. The picture or photo that best illustrates the design points: three-dimensional picture. 5. The bottom surface of this design product is a part that is not easily visible or cannot be seen when in use, so the top view is omitted.

The present invention relates to the technical field of skin care products, and specifically discloses a composition containing superoxide dismutase (SOD) and a preparation method thereof. The composition containing superoxide dismutase (SOD) comprises the following components: 28-32 parts of yeast fermentation product filtrate; 0.8-1.2 parts of garlic bulb extract; 0.8-1.2 parts of superoxide dismutase (SOD); 0.4-0.6 parts of angelica extract; 65-70 parts of water; the preparation method of the yeast fermentation product filtrate is as follows: 1), mix broom, hops, hibiscus bark, and perilla leaves evenly to obtain a plant mixture; 2), mix the plant mixture with water evenly to obtain a plant mixture; 3), inoculate bumblebee Candida albicans and Cryptococcus laurentii into the plant mixture, ferment for 7-9 days, filter, and sterilize to obtain a yeast fermentation product filtrate. The present invention has the advantages of improving the anti-wrinkle and firming effect to achieve rapid wrinkle reduction.

The invention discloses a bumblebee egg-laying induction device and a bumblebee egg-laying inducer, and relates to the technical field of bumblebee breeding. The device comprises a base, an induction mechanism and a honey storage tank; the induction mechanism is arranged on the base, and the induction mechanism comprises a heat-insulating outer layer and at least one egg-laying area located in the heat-insulating outer layer, and a pollen layer is laid in the egg-laying area; the honey storage tank is arranged on the base, and is arranged adjacent to the induction mechanism, and is used to store feed. On the one hand, the device has an egg-laying area, which can induce bumblebees to lay eggs on the pollen layer in the egg-laying area, reduce the physical energy consumed by the bumblebees in building nests, and shorten the egg-laying time; on the other hand, the device can avoid the loss of temperature of the bumblebee egg-laying environment through the arrangement of the heat-insulating outer layer, ensure that the bumblebees lay eggs in a suitable environment, and ensure that the larvae develop in a suitable environment, thereby advancing the time for the bumblebees to form a group; at the same time, through the arrangement of the honey storage tank, the time for the bumblebees to establish the honey pot can also be shortened, thereby further reducing the consumption of the bumblebee's physical energy and shortening the egg-laying cycle.

The invention provides a bumblebee pollination method for tomatoes in a solar greenhouse, and belongs to the technical field of bumblebee pollination. The pollination method comprises the steps of bumblebee food adaptability training feeding, ionone adsorbate spreading and bumblebee releasing for pollination. The method for releasing bumblebee pollination comprises the steps that beta-ionone is not added into sweet water eaten by bumblebees, an outlet of a bear bee hive is opened, the bumblebees naturally fly out of a beehive to complete pollination, and the daytime temperature in a greenhouse is controlled to be 20-30 DEG C in the pollination period; according to the bumblebee pollination method, the bumblebees are high in activeness and good in continuity in the first 30 days, the number of bumblebees entering and exiting a beehive per hour reaches 30-38, the fruit setting rate reaches 92.6-96.1%, the malformed fruit rate is 3.5-4.8%, the average fruit weight is 211.4-226.5 g, and the VC content is 16.4-18.6 mg / 100g.

The present invention relates to the field of bumblebee handling in particular in relation to bumblebee rearing, storage, transport and at the pollination location. For these purposes the invention according to a first aspect provides a housing, such as a nest box, for bumblebee (Bombus spp.) individuals, including bumblebee workers. Further aspects of the invention relate to the use of the housing for confining bumblebees and a method of pollinating blooming plants comprising providing in the vicinity of the plants a number of bumblebee housings according to the invention.

The invention discloses a probiotic composition for regulating intestinal health of bumblebees and application of the probiotic composition. The probiotic composition is prepared from the following raw materials in percentage by mass: 1-2% of bacillus subtilis, 20-30% of sucrose water, 5-8% of yeast powder, 1-2% of honey, 25-40% of apricot pollen, 0.05% of mixed vitamins, 0.05% of a proline solution and the balance of water. According to the probiotic mixture disclosed by the invention, worker bee death caused by escherichia coli infection can be effectively reduced; the feed does not cause the change of the core flora of intestinal microorganisms of bumblebees, namely does not cause the disorder of the intestinal microorganisms, can promote the metabolism of fatty acid, nitrogen metabolism and energy metabolism in intestinal tracts, and is beneficial to the intestinal health of bumblebees.