78 results about "Bumblebee" patented technology

The invention provides a bumblebee attractant and a method for pollinating camellia oleifera by utilizing bumblebees, and belongs to the technical field of camellia oleifera pollination. The bumblebee attractant comprises the following components: eugenol, methyl jasmonate, glucose, a solubilizer and a diluent. The method for pollinating the camellia oleifera by using the bumblebees comprises the following steps: S1, 1-2 weeks before pollination, performing disinfection and insecticidal treatment on a camellia oleifera tree entering row; s2, 2-3 days before flowering of camellia oleifera, bumblebee beehives are placed in a camellia oleifera garden, and hydromel is fed; s3, when male flowers and female flowers of camellia oleifera bloom and the weather is sunny, spraying the bumblebee attractant to stigmas of the female flowers every day, and meanwhile, releasing pollinated bumblebees from the beehive until the flowering phase of the female flowers ends. Eugenol and methyl jasmonate in the attractant can synergistically improve the attraction effect on bumblebees, and the pollination rate of camellia oleifera is increased.

The invention discloses a special feed for breeding bumblebee pollination swarms and a preparation method thereof, and belongs to the technical field of insect feed preparation, and the special feed for breeding the bumblebee pollination swarms comprises the following raw materials: white granulated sugar, glucose, potassium sorbate, epsilon-polylysine, vitamins, trace elements and water; the preparation method comprises the following steps: adding the white granulated sugar and the glucose into boiling water, uniformly stirring, cooling, adding the vitamins, the trace elements, the potassium sorbate and the epsilon-polylysine, uniformly stirring, and cooling to room temperature. The components such as the sugar source, the vitamins and the preservative are introduced to achieve a synergistic effect, so that the freshness of the sugar water is guaranteed, the intake of nutrient substances by the bee colony is improved, the survival rate of the bee colony is effectively increased, the development time of the early-stage bear bee colony is shortened, the growth speed of the early-stage pollination bee colony is accelerated, and the colony potential of the bee colony before pollination is increased.

The invention discloses a bumblebee candida for efficiently producing sophorolipid as well as a construction method and application of the bumblebee candida. According to the invention, metabolic transformation is carried out on a wild type strain of candida bumblebee, a peroxisome membrane transporter gene PXA1 is knocked out, so that a beta-oxidation pathway of fatty acid competing with a sophorolipid synthesis pathway is moderately inhibited, and on the basis, an endogenous potent promoter pENO is utilized to enhance expression of CYP52M1; therefore, the bumblebee candida engineering bacteria for efficiently producing sophorolipid are constructed. Compared with an original strain, the recombinant candida bumblebee has the advantages that efficient conversion of a fermentation substrate and high yield of sophorolipid can be realized, and the sophorolipid yield of shake flask fermentation reaches 88.50 g / L. The strain is fermented in a 5L fermentation tank and fermented for 7 days after rapeseed oil is added, 232.27 g / L of crude sophorolipid is produced, and high productivity is kept.

The invention provides a preparation method of a hydrolyzed collagen product with surface amphipathicity as well as the product and application thereof, and the preparation method comprises the following steps: (1) taking hydrolyzed collagen enzymatic hydrolysate as a substrate, then preparing a fermentation culture medium with vegetable oil and sugar, and fermenting by adopting candida bumblebee; (2) after fermentation, adding lysozyme into the fermentation liquor for activating treatment, and then sterilizing, centrifuging and filtering; and (3) carrying out freeze-drying treatment on the filtered fermentation liquor to obtain the hydrolyzed collagen product with surface amphipathicity. The hydrolyzed collagen product prepared by the invention not only has low molecular weight and high permeability to skin, but also has water locking and moistening effects on the skin surface due to the amphiphilic sophorolipid on the surface, so that the moisture retention of the skin is better improved, and the product has excellent product characteristics and has wide market prospects.

The invention relates to an eDNA method for identifying bumblebee honeycombs in permafrost regions of Qinghai-Tibet Plateau. The eDNA method comprises the following steps: S1, positioning observation sample belts and arranging sampling points on the basis of multi-source data in combination with a Bayesian hierarchical model; s2, performing deep freezing and complete excavation on matrixes around and inside all the sampling points; s3, stratified sampling and anti-degradation treatment are carried out, and a spatial attribute database is established; s4, extracting and purifying eDNA (enhanced deoxyribonucleic acid); s5, determining a nest host based on PCR amplification, high-throughput sequencing and sequence comparative analysis; and S6, constructing a Bayesian hierarchical model in combination with biological information, and judging honeycomb characteristics. The method is simple, rapid and low in cost, not only is suitable for Qinghai-Tibet Plateau permafrost regions, has the core advantages of non-destructiveness and high sensitivity, but also is also suitable for underground insect nest identification research in other environment sensitive regions or regions difficult to physically explore in principle, and has wide application prospects. The method is especially suitable for monitoring rare, secret or difficult-to-approach organisms.

The invention discloses a method for promoting development of swallowing glands of bumblebees, the method is characterized in that the mass ratio of linoleic acid to alpha-linolenic acid in bumblebee feed is adjusted to be (1-5): 1, and the development of the swallowing glands of the bumblebees is promoted, the method is simple and easy to implement, and by adjusting the ratio of the linoleic acid to the alpha-linolenic acid in the bumblebee feed, the development of the swallowing glands of the bumblebees is promoted. According to the method, normal development of the giant bumblebees is guaranteed, diet preference of the giant bumblebees is met, nutrient fluctuation of natural pollen is made up, development of swallow glands can be remarkably promoted, feeding of larvae of the giant bumblebees is facilitated, and growth and development of offspring of a bee colony are facilitated.

The invention relates to the technical field of plant planting, in particular to a method for promoting flowering and fruit setting of amomum tsao-ko and application thereof.In the vigorous vegetative growth period, a nutrient solution composed of ammonium sulfate, NAA and GA3 with the proper concentration is adopted, the vigorous vegetative growth period can be effectively prolonged, and therefore flower bud differentiation is inhibited; a nutrient solution B composed of monopotassium phosphate, ABA and BR is sprayed in April, vegetative growth can be effectively broken through to be converted into reproductive growth, flower buds are promoted to be rapidly differentiated and formed when a rainy season comes, the flowering rate can be increased by 28-33%, the yield of the amomum tsao-ko medicinal material can be greatly increased, bumblebees are quantitatively put in the early stage of May, and the pollination rate can be greatly increased; in the blooming period, the nutrient D is spread around the cluster buds, meanwhile, the nutrient solution E is sprayed in a matched mode, and the fruit setting rate can be increased by 8.9%-9.6% compared with the fruit setting rate.

The utility model discloses a semi-closed greenhouse beehive device, which belongs to the technical field of semi-closed greenhouse beehives and is mounted below a substrate groove and in front of an air outlet of an air duct. The beehive device comprises a hoisting mechanism, a support and a beehive body. The lifting mechanism comprises a hook and a lifting frame connected with the hook in a hooked mode, the lifting frame is connected with the support, and the beehive body is installed on the support. The beehive device is redesigned, firstly, temperature rise caused by sunlight is reduced through shading of plants and shielding of an upper building, secondly, the ventilation condition of the beehive is improved through air outlet of the air duct, the surrounding environment condition is adjusted, and then the activity degree of bumblebees is guaranteed.

The invention belongs to the technical field of artificial insemination of bumblebees, and particularly relates to a male bumblebee sperm collecting device. The male bee seminal vesicle extraction platform is arranged on the base, the male bee seminal vesicle extraction platform is used for clamping the male bee seminal vesicle glass sheet, the male bee seminal vesicle glass sheet is used for placing a male bee seminal vesicle, and the male bee seminal vesicle extraction platform drives the male bee seminal vesicle glass sheet to move in the horizontal direction of the base; the insemination needle fixator is vertically arranged on the base in a lifting mode and used for fixing a glass needle, and the glass needle faces the male bee seminal vesicle glass sheet. Through the device, the extraction efficiency can be remarkably improved, so that the sperm exposure time is effectively shortened, and relatively high vitality is kept.

The present invention relates to mutants of the bumblebee Yarrowia SBLE enzyme having modulated transesterification and / or esterification activity compared to the bumblebee Yarrowia SBLE enzyme, and to homologues of the bumblebee Yarrowia SBLE enzyme, such as the C. bogoriensis LE enzyme, the B. batistae LE enzyme and the R. riograndensis LE enzyme, having transesterification and esterification activity on bolaform glycolipids. The present invention also provides genetically modified yeast strains comprising a mutant LE enzyme or a bumblebee Yarrowia SBLE enzyme homologue of the present invention. The present invention further provides the use and methods of the mutant LE enzymes, the bumblebee Yarrowia SBLE enzyme homologues and the modified yeast strains of the present invention for the production of acidic glycolipids, lactone-form glycolipids and / or glycolipid oligomers / polymers / esters. The present invention also provides the three-dimensional structure of the bumblebee Yarrowia SBLE enzyme and its use for the design of mutants having modulated hydrolysis and / or transesterification activity.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

A technology for relieving diapause of bumblebee queen bees is realized mainly by performing worker bee induction at key nodes of bumblebee queen breeding activation: after mating bumblebee queen bees are activated, selecting a worker bee which just comes out of a house for one day from a bee supply colony to join a queen bee feeding group, inducing the queen bees to relieve diapause, and entering a breeding state; after 5-7 days, replacing the worker bees added before with new worker bees to prevent the worker bees from laying eggs; the process is repeated for two to three times until the queen bees lay eggs; through worker bee induction, bumblebee queen diapause can be quickly relieved, the queen bee is stimulated to lay eggs in advance, and the breeding period can be shortened by about three months; the bumblebee queen is not subjected to low-temperature diapause, so that the vigorous vitality of the bumblebee queen is kept, the breeding efficiency of the bumblebee queen is improved, and the seed selection and breeding process is accelerated.

The present invention discloses a method and device for predicting the fighting behavior of bumblebees. The method comprises: obtaining a training sample set, wherein the training sample set is a plurality of photos of bumblebee colonies marked with label categories; identifying the training samples of the training sample set according to a fighting recognition model, and obtaining a polar coordinate system of the bumblebee outline, wherein the fighting recognition model is a residual convolutional neural network model fused with an attention mechanism in a pyramid structure; determining the training prediction result of the fighting recognition model according to the polar coordinate system, and determining that the fighting recognition model has completed training when the training prediction result reaches a preset target; and identifying bumblebees with fighting behavior according to the fighting recognition model that has completed training. The prediction method uses a primary classification obtained by the fighting recognition model and a secondary classification obtained based on the bumblebee outline to jointly predict the fighting situation of the bumblebees at the location. The prediction method combines the bumblebee picture features and the bumblebee outline features, thereby improving the accuracy of predicting the bumblebee fighting behavior.

The invention relates to a bumblebee hive monitoring system that controls the activity of the bumblebees in the hives by providing metrics regarding the greenhouses to be pollinated, such as temperature and relative humidity, thereby ensuring the productivity of the pollination and of the agricultural operation. The system comprises at least one main module (1) formed by a case (2) in the form of a box with an open upper face (16) and which is closed by a cover (3), the module containing a hive (17) and having a hole (12) in a side face thereof aligned with the opening of the exit from the hive (17), the module (1) being equipped with temperature sensors (5, 6) and relative humidity sensors (7, 8) and with fans (9), connected to an electronic controller (4), the fans (9) being activated according to the temperature and humidity parameters in order to maintain an optimal climate in the hive.

A trap 1 for catching insects, such as Asian hornets, with a receptacle for receiving an insect attractant. The bait may be liquid and the receptacle may hold a volume of water. There is a closure 3 which may be releasably fastened 12 to the receptacle. The closure has a recessive surface, such as a cone or funnel, which extends into the receptacle, and an aperture 9 for allowing an insect into the receptacle. There are a plurality of fins, walls or partitions 30 on the receptacle, to guide the insect to the aperture. The fins may be upstanding, radially disposed on the funnel, or trapezoidal in shape. There may be bait odour vents on the recessive surface. There may be bycatch holes 10 to release smaller non-target insects. The trap may have feet 11 or an oversized closure so it can be rested horizontally on its side. The aperture may lead to an L shaped hollow elbow internal the receptacle, for reducing escape of the insects. The elbow may be perforated to allow odour through. A method of using the trap near beehives is disclosed. The trap may be immersed in water to exterminate trapped insects.

1. Name of the product in this design: Drone Remote Controller (Little Bumblebee Series). 2. Purpose of this design: For use as a drone remote controller. 3. The key design feature of this product is its shape. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The invention relates to the technical field of microorganisms, in particular to bumblebee Starmomyces sp. And application of the bumblebee Starmomyces sp. In preparation of glycolipid with anti-aging and / or moisturizing functions. The invention provides bumblebee raw Starmonia yeast with the preservation number of CCTCC (China Center For Type Culture Collection) NO: M 2025061, and provides a preparation method of glycolipid. When being used for preparing glycolipid, the strain has the advantages of short fermentation time, high grease utilization rate and high glycolipid yield. Moreover, the glycolipid obtained by the method is a mixed glycolipid of sophorolipid, glycerol glycolipid and glucose lipid, and can be widely applied to the fields of medicines, foods, agriculture, environmental protection, cosmetics and the like, and the production cost of the glycolipid can be greatly reduced.

The invention provides a bumblebee pollination method for tomatoes in a solar greenhouse, and belongs to the technical field of bumblebee pollination. The pollination method comprises the steps of bumblebee food adaptability training feeding, ionone adsorbate spreading and bumblebee releasing for pollination. The method for releasing bumblebee pollination comprises the steps that beta-ionone is not added into sweet water eaten by bumblebees, an outlet of a bear bee hive is opened, the bumblebees naturally fly out of a beehive to complete pollination, and the daytime temperature in a greenhouse is controlled to be 20-30 DEG C in the pollination period; according to the bumblebee pollination method, the bumblebees are high in activeness and good in continuity in the first 30 days, the number of bumblebees entering and exiting a beehive per hour reaches 30-38, the fruit setting rate reaches 92.6-96.1%, the malformed fruit rate is 3.5-4.8%, the average fruit weight is 211.4-226.5 g, and the VC content is 16.4-18.6 mg / 100g.

The present invention relates to the field of bumblebee handling in particular in relation to bumblebee rearing, storage, transport and at the pollination location. For these purposes the invention according to a first aspect provides a housing, such as a nest box, for bumblebee (Bombus spp.) individuals, including bumblebee workers. Further aspects of the invention relate to the use of the housing for confining bumblebees and a method of pollinating blooming plants comprising providing in the vicinity of the plants a number of bumblebee housings according to the invention.

The invention discloses a probiotic composition for regulating intestinal health of bumblebees and application of the probiotic composition. The probiotic composition is prepared from the following raw materials in percentage by mass: 1-2% of bacillus subtilis, 20-30% of sucrose water, 5-8% of yeast powder, 1-2% of honey, 25-40% of apricot pollen, 0.05% of mixed vitamins, 0.05% of a proline solution and the balance of water. According to the probiotic mixture disclosed by the invention, worker bee death caused by escherichia coli infection can be effectively reduced; the feed does not cause the change of the core flora of intestinal microorganisms of bumblebees, namely does not cause the disorder of the intestinal microorganisms, can promote the metabolism of fatty acid, nitrogen metabolism and energy metabolism in intestinal tracts, and is beneficial to the intestinal health of bumblebees.

The invention provides a bumblebee raising device, and relates to the technical field of raising devices, the bumblebee raising device comprises a bumblebee raising box body, one end of the bumblebee raising box body is movably installed in one end of a rapid connection mechanism, a ventilation mechanism is arranged in a bumblebee main nest, and a communication pipeline is arranged in the center of the bumblebee raising box body; a heat dissipation mechanism is arranged on one side of the bumblebee feeding box body, one end of a bumblebee main nest is arranged in a quick connecting mechanism, at the moment, the quick connecting mechanism can clamp the surface of the bumblebee main nest for quick installation, and meanwhile the bumblebee main nest can be independently taken out when the internal condition needs to be checked; the heat dissipation mechanism arranged on one side of the bumblebee feeding box body can be opened according to requirements, so that the temperature in the bumblebee feeding box body is adjusted, the ventilation environment in the bumblebee main nest can be adjusted according to requirements through the ventilation mechanism arranged in the bumblebee main nest, and the excrement receiving plate which can be movably taken out can be conveniently cleaned.

This application discloses a bumblebee feed for artificial breeding and its preparation method, relating to the field of feed technology. It addresses the problem of unclear dosage of potassium sorbate in bumblebee feed, leading to uncontrollable preservative effects or adverse effects on bumblebees. The feed consists of a feed base and potassium sorbate as a preservative, with a mass percentage of 0.08%–0.12%. The preparation method involves mixing potassium sorbate with the feed base to achieve a final mass percentage of 0.08%–0.12% in the feed. The mixing time is 5–30 minutes, and the mixing temperature is 15–30°C. A mother liquor method is used for sugar water feed, and a premixing method is used for pollen feed. This application experimentally determined a specific safe dosage of 0.1%, which can keep the feed fresh and mold-free for one week under conditions of 28°C and 55% humidity, ensuring safety for bumblebees and providing a directly applicable preservative feed technology solution for commercial bumblebee breeding.

The present invention relates to the technical field of the installation of bumblebee hives, and specifically relates to a sunshade and cooling device for bumblebee hives in a multi-functional glass greenhouse. The sunshade and cooling device for the beehive includes a vertical column and a side plate. The beneficial effects are as follows: Through the cooperation of a semi-circular rotating rod, a movable ring, and a folding sunshade net, the purpose of rotating and adjusting the protection angle of the folding sunshade net is achieved. By setting a pair of pulling ropes distributed front and back, the purpose of conveniently pulling the folding sunshade net to move back and forth for adjustment is achieved. The pulling ropes extend to the lower end of the fixed rod, facilitating the adjustment operation. Thus, by adjusting the folding sunshade net, the adaptation to the environment is realized. In summer, both sides of the folding sunshade net are covered for sunshade. In winter, the folding sunshade net is completely stored for temperature adjustment. When spraying drugs, the folding sunshade net completely covers and protects the beehive.

The invention discloses a low-voltage flexible DC micro-grid fault positioning method based on an improved Tiancattle swarm algorithm. According to the method, the fault point can be accurately positioned when the inter-electrode short-circuit fault or the single-electrode grounding fault occurs in the flexible direct-current power distribution network. The method is slightly influenced by excessive resistance, and a fault positioning problem is converted into a fault parameter identification problem. And in combination with the improved Tiancattle swarm algorithm, the accuracy of fault point positioning can be improved, errors are reduced, and the information distortion resistance is improved. The method is of great significance in improving the safety and reliability of the direct-current power distribution system.

The utility model relates to the technical field of bee breeding, in particular to a bumblebee species cultivating device which comprises a box body, lining plates are attached to the inner wall of the box body, a partition plate is connected between every two opposite lining plates and divides the box body into a feed area and a spawning area, the lining plates and the partition plate are both made of wood, and the partition plate is made of wood. A partition plate is arranged in the box body, a through opening for bumblebees to pass through is formed in the partition plate, a ventilation opening is formed in the side, away from the feed area, of the box body, a first sealing cover plate and a second sealing cover plate are arranged on the feed area and the egg laying area in a clamped mode respectively, a water tank is connected into the feed area, and a supply assembly for supplying sweet water at regular time is arranged on the first sealing cover plate. A temperature and humidity control system used for controlling temperature and humidity is arranged in the spawning area, and the inner wall of the spawning area is coated with natural beewax. The device has the effects of improving the matching degree of the breeding device and the nest environment and improving the problem that sweet water is prone to going bad.

The present invention discloses a method for constructing a bumblebee model of acute or chronic neuropathy. The method uses newly emerged bumblebees as the subjects for establishing the bumblebee model of neuropathy; acrylamide is dissolved in a sterile sucrose solution to prepare an acrylamide sucrose solution; the newly emerged bumblebees are fed the acrylamide sucrose solution to obtain the bumblebee model of neuropathy; the model constructed by the method of the present invention can be used to screen methods or drugs for preventing or treating neurotoxicity-related diseases, can be applied to explore the specific mechanisms of nervous system-related diseases, and has good application prospects.

The present application relates to the technical field of fermentation detection, and particularly relates to a camellia oil fermentation detection method of bear-born candida, which comprises the following steps: preparing camellia oil emulsion; mixing the camellia oil emulsion with bear-born candida seed liquid, and feeding gas to obtain fermentation liquid; detecting the stirring shaft torque of the fermentation tank; adjusting the feeding rate of the camellia oil emulsion; detecting the attenuation rate of the volume oxygen transfer coefficient of the fermentation liquid after the feeding rate is adjusted; adjusting the aeration mode of the fermentation liquid, including adjusting the gas-liquid contact time or carrying out pulse aeration; continuously monitoring the oil-water liquid level potential of the fermentation liquid after the aeration mode is adjusted; determining whether the fermentation liquid reaches the fermentation end point according to the change curve of the oil-water liquid level potential; if the fermentation liquid reaches the fermentation end point, determining the stirring speed of the stirring shaft; and obtaining the bear-born candida after fermentation. The present application realizes the improvement of the accuracy of fermentation process control.