25 results about "Pollen" patented technology

This invention provides a method and system for quantitative identification of fluvial and lacustrine facies based on paleontological ecological habit reconstruction, comprising: collecting target samples from a target area within a target well; determining that the target samples do not contain ostracods and gastropods; obtaining the plant species of aquatic plant pollen, the algal species of algal pollen, the quantity of plant pollen of each aquatic plant genus, and the quantity of algal pollen of each algal genus in the target samples; quantitatively calculating paleoenvironmental identification indicators based on the plant species, algal species, plant pollen quantity, and algal pollen quantity; and determining the fluvial and lacustrine sedimentary environment of the target area based on the paleoenvironmental identification indicators. This invention overcomes the drawbacks of traditional qualitative methods that are "highly subjective and rely on experience-based judgment," is more effective, does not require extensive and expensive geochemical analysis and testing, is not limited by specific oxidation / reduction environments of strata or subsequent alterations, and has wider applicability.

The present invention relates to the technical field of bee breeding, and particularly relates to a method for mixed feeding of Italian bees and Chinese bees. The method for mixed feeding of Italian bees and Chinese bees comprises the following steps: S1: Raise the Italian bee colony at one side position of a double-queen colony beehive; S2: After 2 to 3 days, transfer the Chinese bees into the beehive and raise them at the other side position, and an adjustable queen excluder is installed in the middle of the beehive to separate the two bee colonies; S3: Open both nest entrances, and both bee colonies can freely enter and exit their respective nest entrances, recognize the hive, and collect; S4: After raising for a period of time, adjust the aperture of the queen excluder to 0.2 cm and spray a bee colony odor reduction liquid; S5: After 3 to 5 days, adjust the aperture of the queen excluder to 0.38 cm; S6: After a period of time, remove the escape prevention piece on the nest entrance of the Chinese bee colony to make it more free for the Chinese bees to enter and exit; S7: Add a super in the season with sufficient honey and pollen sources. The present invention provides a method for mixed feeding of Italian bees and Chinese bees, which can not only improve the mite resistance of the Italian bee colony, but also reduce the risk of the Chinese bee colony being infected with wax moths.

The invention provides an induction culture method for embryonic calluses of pinus khasys, and relates to the technical field of biologication.The method comprises the steps that male flowers of the pinus khasys are collected, and the collected male flowers are subjected to surface disinfection treatment; soaking the male flowers of the pinus khasys subjected to surface disinfection in a detoxification solution, and performing detoxification treatment; cleaning the detoxified male flower explant of pinus khasys, and absorbing moisture; and taking out the explant of the pinus khasys after moisture absorption, separating out a male gametophyte of the explant, and placing the male gametophyte in a culture medium for dark culture until callus is induced. According to the method, the male gametophyte of the pinus khasys is adopted for explant cultivation of the pinus khasys, anther serves as a reproductive organ of the plant, genetic information is stable, and the good characters of the pinus khasys can be kept; by selecting the pinus kesiya anther as a breeding material, stable separation of offspring can be realized as early as possible, and the reliability and accuracy of selection are improved; in addition, the calluses induced by the pollen can maintain the excellent characters of the female parent, and have important significance on subsequent superior plant propagation.

The invention provides a cattail pollen content prediction method and system based on a hyperspectral double-flow multi-scale CNN, and belongs to the field of quality detection of traditional Chinese medicinal materials. The method comprises the following steps: acquiring hyperspectral image data of a pollen typhae sample, preprocessing the acquired hyperspectral image data, and extracting region-of-interest spectral data of the pollen typhae sample; inputting the extracted spectral data of the region of interest into a trained double-flow multi-scale residual fusion CNN model, performing multi-scale feature extraction and fusion by using a VNIR multi-scale branch and an NIR multi-scale branch, and outputting multi-scale fusion features; inputting the multi-scale fusion features into an attention fusion module, and outputting final fusion features; and based on the final fusion features, outputting a content prediction value of the active ingredients of the pollen typhae through a content prediction layer. The method solves the problems that a traditional detection method is low in efficiency, samples are damaged, and an existing spectrum model ignores wave band characteristic differences, so that precision is insufficient, and rapid, lossless and high-precision prediction of the pollen typhae content is achieved.

The invention, concerning the field of plant genome editing, relates to a method of transforming pollen grains, a method of genome editing pollen grains, and a method of obtaining a genome edited homozygous plant.

This paper provides a method for restoring paleoclimate factors based on plant community homeostasis. This method, which falls within the field of paleoclimate restoration in paleontology and geophysics, focuses on characterizing paleotemperatures and paleoprecipitation. Specifically, it relates to a method for restoring pollen-paleoclimate factors (paleotemperatures and paleoprecipitation) from wells or rock columns, comparing the measured terrestrial pollen species and content with indicators of the climatic conditions required for the reproduction of terrestrial plant species and communities, and incorporating corrections based on paleolatitude insolation and evaporation intensity. This method achieves the restoration of a full-sequence, continuous paleoclimate curve, and the restored paleoclimate factors are more accurate.

The invention relates to the technical field of traditional Chinese medicine, and discloses a preparation method and a quality control method of radix trichosanthis. Adding water, grinding into a radix trichosanthis medium powder suspension, and filtering the obtained radix trichosanthis medium powder suspension with a 80-100-mesh screen to obtain a radix trichosanthis filtrate; taking the radix trichosanthis filtrate, putting the radix trichosanthis filtrate into a 200-350-mesh filter bag, suspending until no water drops, adding water, and filtering until the content of residual protein in the filtrate is lower than a control level, so as to obtain a radix trichosanthis filter cake; adding water into the radices trichosanthis filter cake, uniformly stirring, and removing supernate to obtain purified radices trichosanthis; drying the cleaned radix trichosanthis at low temperature to obtain dried radix trichosanthis; scattering, sieving and drying to obtain a radix trichosanthis finished product. By optimizing the steps of grinding, filtering, precipitation purification and temperature-controlled drying, the preparation time of the radix trichosanthis is shortened, the yield is increased, the dispersity of the radix trichosanthis in water is improved, a residual protein mass control method is established, and the preparation process level and the product quality of the radix trichosanthis are improved.

The application discloses a kind of based on location-bound information guidance image object segmentation method belongs to computer vision field.The present application obtains pollen segmentation image by combining the information of two modules: target positioning module obtains positioning map by the classification network trained by image level label, provides accurate location information;Boundary guidance module utilizes pollen contour prior knowledge to match target pollen to obtain saliency map, obtains fine-grained boundary information.This method can make full use of the complementary relationship between the two, obtain accurate target pollen boundary, and discard non-target object (such as impurity) pixel, significantly improve pollen image segmentation quality.

The present application relates to a kind of UPLC characteristic atlas of Typhae Pollen medicinal material construction method and detection method.The present application is by using ultra-high performance liquid chromatography, and chromatographic condition is reasonably controlled, find out while applicable to Typhae Pollen medicinal material different base (water candle cattail and oriental cattail) Ultra-high performance liquid chromatography condition, to construct the UPLC characteristic atlas of Typhae Pollen medicinal material.The water candle cattail characteristic atlas of the present application contains 9 characteristic peaks, and the oriental cattail characteristic atlas contains 10 characteristic peaks, this method is strong in special property, high in stability, good in repeatability, can be used to distinguish water candle cattail and oriental cattail these two base, also can be used for the identification of Typhae Pollen sample, realizes the purpose of effective identification Typhae Pollen multi-base, simultaneously helps to perfect the quality control standard of Typhae Pollen medicinal material.

The present invention belongs to the field of traditional Chinese medicine detection, and specifically relates to the chromatographic identification of Typhae Pollen charcoal. Typhae Pollen charcoal in Fuke Duanhongyin capsules is identified using thin-layer chromatography. Identification bands are detected in both the test sample and the reference sample, isorhamnetin-3-O-neohesperidin solution, with good separation between the bands. In the negative control, no characteristic bands are detected in Fuke Duanhongyin capsules lacking Typhae Pollen charcoal, indicating no interference. The chromatographic identification method of Typhae Pollen charcoal has strong specificity and good durability, and is applicable to different thin-layer plates, at different temperatures and humidity levels. It can also be quickly and accurately identified even in high-humidity environments, and can be effectively used to identify Typhae Pollen charcoal in Fuke Duanhongyin capsules.

The invention relates to a method for judging the degree of heating of pollen typhae processing, in particular to a method for judging the degree of heating of pollen typhae processing based on multi-source data fusion and a lightweight attention mechanism. In view of the remarkable advantages of automatically extracting deep features and establishing nonlinear association among multi-source information through a cross-modal interaction mechanism in the process of processing high-dimensional heterogeneous data fusion by an emerging deep learning method, the invention provides a pollen typhae processing fire judgment method based on multi-source data fusion and a lightweight attention mechanism. According to the method, near infrared spectrum (chemical components), electronic nose (smell) and electronic eye (color) data are fused for the first time and applied to cattail pollen processing fire judgment; meanwhile, the deep learning algorithm SAINT is subjected to lightweight improvement for the first time, and the purpose is to construct a pollution-free, lossless and rapid pollen typhae charcoal processing quality multi-dimensional intelligent comprehensive judgment model capable of achieving real-time online monitoring.

The invention discloses a breeding and identification method of double-low photo-thermo-sensitive dual-purpose genic male sterile line rice, and belongs to the field of rice breeding. The method specifically comprises the following steps: respectively carrying out constant-temperature treatment at 23.0 + / -0.1 DEG C and 19.0 + / -0.1 DEG C for 10 days from a pollen mother cell formation stage to a meiosis stage, taking out a sterile line material after the treatment, and placing the sterile line material under a natural light temperature condition; the identification method of the double-low photo-thermo-sensitive dual-purpose genic male sterile rice comprises the following steps: placing anther on a glass slide during flowering, mashing the anther with tweezers in 0.1% I2-KI liquid, observing pollen fertility under a microscope, and treating at 23.0 DEG C for 10d to obtain pollen dyeable rate lt; the dyeable rate gt of the pollen under the conditions that the pollen is treated at 19.0 DEG C for 10 days is 1%; 70% of the sterile line is a double-low dual-purpose genic sterile line. Hybrid seed production and sterile line seed propagation of the double-low photo-thermo-sensitive dual-purpose genic male sterile line bred by the method are both safe, and sustainable development of two-line method hybrid rice is promoted.

The invention relates to a living body identification method for a flower bud differentiation period of China rose. The method for identifying the flower bud differentiation period of the China rose in vivo comprises the following steps: (1) determining the position of a mixed bud of an annual branch or an annual branch, and segmenting the mixed bud; the method comprises the following steps: dividing three sections from top to bottom according to morphology, namely an upper part, a middle part and a lower part, and selecting mixed buds at the upper part; (2) determining the growth period of the upper mixed buds according to the number of bud scales, the opening degree of the bud scales and the leaf extension condition of the upper mixed buds; and 3) determining the flower bud differentiation period of the flower bud according to the leaf extension number and the leaflet expansion degree of the flower bud. The technical problem to be solved is that the flower bud at the upper part of the annual branch or the annual branch is subjected to living body observation by naked eyes or a magnifying lens, the flower formation transformation and flower bud differentiation period can be distinguished, and the flower bud cannot be damaged.

The invention, concerning the field of plant genome editing, relates to a method of transforming pollen grains, a method of genome editing pollen grains, and a method of obtaining a genome edited homozygous plant.

The utility model provides a collecting device for cotton breeding, and relates to the technical field of cotton breeding collection, the collecting device comprises a collecting mechanism, one side of the outer wall of the collecting mechanism is fixedly connected with a neck protection mechanism, the collecting mechanism comprises a connecting fixing block, and the inner surface wall of the connecting fixing block is fixedly connected with a connecting pad. According to the male parent flower collecting device, under the action of the collecting mechanism, the whole collecting device is fixed to the body of a worker through the binding band, and meanwhile, the tool containing box and the water cup containing box are arranged, so that the worker can conveniently store and use tools in the process of collecting male parent flowers; and meanwhile, a worker can conveniently supplement water after working for a long time, the worker can carry a large number of collecting boxes at a time by arranging a connecting plate and placing the collecting boxes in the notches, the frequency of taking powder back and forth is reduced, a large amount of physical strength is saved, and meanwhile the powder taking efficiency is improved.

The utility model discloses a breeding pollination device which comprises a powder spraying pipe, a spraying pipe arranged at the front end of the powder spraying pipe, a powder blocking cover arranged on the outer side of the spraying pipe, a piston arranged in the spraying pipe, a piston rod connected with one end of the piston, a powder storage pipe connected with one side of the powder spraying pipe through a connecting rod, a pipe cover movably installed on the top of the powder storage pipe, and a transparent material made of a transparent material. The powder blocking cover comprises a first fixed pipe section, a telescopic corrugated pipe section and a second fixed pipe section which are connected in sequence, and the first fixed pipe section is fixed on the powder spraying pipe; the pollen blocking cover is made of a transparent material, so that the pollination condition of the spray pipe can be conveniently observed from the outside, and the pollen blocking cover is prevented from blocking the pollination sight of a worker; the pollen blocking cover comprises the first fixed pipe section, the telescopic corrugated pipe section and the second fixed pipe section which are connected in sequence, so that the pollen blocking cover can stretch and retract to a certain extent during pollination, the length of the pollen blocking cover is adjusted, and the situation that the pollen blocking cover blocks the spray pipe to spray powder due to blocking of external branches and leaves is avoided.

This invention proposes a method and system for predicting the content of Typha pollen based on a hyperspectral dual-stream multi-scale CNN, belonging to the field of quality detection of traditional Chinese medicinal materials. The method includes: acquiring hyperspectral image data of Typha pollen samples; preprocessing the acquired hyperspectral image data and extracting the region of interest (ROI) spectral data of the Typha pollen samples; inputting the extracted ROI spectral data into a trained dual-stream multi-scale residual fusion CNN model, using VNIR and NIR multi-scale branches for multi-scale feature extraction and fusion, and outputting multi-scale fused features; inputting the multi-scale fused features into an attention fusion module, and outputting the final fused features; based on the final fused features, outputting the predicted content of the effective components of Typha pollen through a content prediction layer. This invention solves the problems of low efficiency, sample destruction, and insufficient accuracy caused by existing spectral models ignoring band characteristic differences in traditional detection methods, achieving rapid, non-destructive, and high-precision prediction of Typha pollen content.

This invention discloses a pollen-like imidacloprid nanopesticide, its preparation method, and its application. The preparation method includes: mixing solid silica spheres, water, copper nitrate, and ammonia solution, and then carrying out a hydrothermal reaction at 120-140℃; the mass ratio of the solid silica spheres to ammonia in the ammonia solution is 1:8 to 1:20; rinsing the product in hydrochloric acid to obtain pollen-like, rough-surfaced hollow silica; dissolving sucrose in water to form a sucrose aqueous solution; dissolving imidacloprid in acetone, adding the pollen-like, rough-surfaced hollow silica, stirring, and then rotary evaporating; adding the sucrose aqueous solution and rotary evaporating again. Compared with commercially available imidacloprid water-dispersible particles, the pesticide of this invention has 2.1 times the adhesion ability on pakchoi leaves. Under simulated rainfall conditions, the leaf retention rate of the pollen-like nanopesticide is 4.8 times that of commercially available imidacloprid water-dispersible particles. After 8 hours of UV irradiation, the imidacloprid residue rate of the pesticide of this invention is still as high as 76.8%.

This invention belongs to the field of biological tissue separation technology, and particularly relates to a method for separating and collecting rice anther tissue. The method includes: 1) Sampling: After removing the husks from a rice panicle, the anthers are peeled off, using the anthers as a sample; 2) Pollen extraction: The sample is mixed with Hank's incubation solution, and the anther tissue is mechanically broken. After the anther wall is completely ruptured, pollen grains are released to obtain a sample solution; 3) Separation of pollen grains and anther wall: The sample solution is filtered in stages to separate pollen grains and anther wall. The filtrate is centrifuged to separate pollen grains and anther chamber fluid, thus completing the separation and collection of rice anther tissue. This invention allows for highly mechanized operation, saving manpower, improving the efficiency of separating and collecting anther tissue, significantly improving the purity of pollen grains, and controlling the loss rate to a low level.

The application provides a quantitative recovery method of paleoclimate based on spore and pollen data, which comprises the following steps: step 1, establishing a basic database of paleo-pollen; step 2, establishing a comprehensive database of ecological and climatic conditions of paleo-pollen; step 3, reconstructing a paleoclimate distribution model tending to normal distribution; step 4, checking the distribution shape of the average distribution data model; step 5, normalizing the data model; and step 6, recovering the characteristics of the paleoclimate conditions. The quantitative recovery method of paleoclimate based on spore and pollen data has strong adaptability and expandability, can be combined with the discrimination of the source of the early spore and pollen, adjust the scale of the confidence interval based on the proportion of the original and external spore and pollen, and further eliminate the interference of the external spore and pollen with different degrees on the recovery of the paleoclimate. The method has clear and clear principles, quantifies the recovery of the paleoclimate, and has high accuracy and operability.

This invention provides a method for inducing and culturing embryogenic callus from *Pinus kesiya*, relating to the field of biotechnology. The method includes: collecting male cones of *Pinus kesiya* and performing surface disinfection on the collected male cones; immersing the surface-disinfected male cones in a detoxification solution for detoxification; washing the detoxified male flower explants and drying them; removing the dried explants, separating the male gametophytes from the explants, and culturing them in the dark in a culture medium until callus is induced. This method uses male gametophytes of *Pinus kesiya* for explant cultivation. Anthers, as reproductive organs of plants, possess stable genetic information, which helps maintain the superior traits of *Pinus kesiya*. Using *Pinus kesiya* anthers as breeding material allows for early and stable segregation of offspring, improving the reliability and accuracy of selection. Furthermore, the callus induced by pollen can maintain the superior traits of the maternal parent, which is of great significance for subsequent propagation of superior plants.

The present application belongs to the technical field of oil and gas exploration, and particularly relates to a method for quantitatively dividing and contrasting red beds by spore-pollen facies. The method comprises the following steps: collecting samples; adding dyed modern lycopodium spore pieces to the collected samples respectively, and preparing slices; identifying and counting the particle numbers of each organic matter type and the numbers of modern lycopodium spores in the sample slices; calculating the amorphous organic matter abundance and total organic matter abundance of each sample, and drawing the abundance variation curves respectively; finding the maximum division point according to the variation curves of the amorphous organic matter abundance and the total organic matter abundance, and establishing the standard for dividing red beds. The present application realizes the division and isochronal correlation of red beds, effectively solves the problems existing in the division and correlation of red beds by other methods, has the advantages of strong practicability, strong generalizability and the like, and can provide the most basic and most important basis for fine exploration of red beds, and has achieved good effects in practical application.

The invention discloses a pollen trap without hurting bees, and relates to the technical field of bee breeding equipment, the pollen trap comprises a bottom plate, the left and right sides of the top of the bottom plate are clamped with side plates with symmetrical and same structures, the tops of the two side plates are clamped with a top plate, the front sides of the two side plates are rotationally clamped with a pollen trap front plate, and the pollen trap front plate is provided with a front bee channel; a pollen removing rear plate is arranged on the rear side of the pollen removing front plate and provided with a rear bee channel, a mounting plate is arranged on the rear side of the top of the bottom plate, a partition plate is arranged at the position, between the pollen removing rear plate and the mounting plate, of the top of the bottom plate, and a steel wire strip is arranged on the rear side wall of the pollen removing front plate. The surfaces of the front bee channel and the rear bee channel of the pollen-removing front plate and the pollen-removing rear plate are designed in a step shape and a slope shape, the mounting columns and the positioning protruding blocks are arranged on the rear side wall of the pollen-removing front plate, the mounting columns at the two ends position the steel wire strip when the steel wire strip is mounted, friction generated when a bee colony enters and exits the pollen-removing device is reduced, and the pollen-removing effect is improved. The bee body injury to the bee colony due to friction is reduced.

The invention relates to the field of oil and gas exploration and development, and discloses a hydrocarbon source rock sporopollen paleoclimate reconstruction method and device for a neonatozoic basin. According to the method, sporopollen identification can be carried out on a plurality of hydrocarbon source rock samples, sporopollen identification data of each hydrocarbon source rock sample is determined, namely, a plurality of sporopollen categories in each hydrocarbon source rock sample and relative contents corresponding to different sporopollen categories are determined, and sporopollen identification is carried out on the basis of the sporopollen identification data of each hydrocarbon source rock sample and the created sporopollen paleoclimate database. The method comprises the following steps: determining qualitative paleoclimate parameter data and quantitative paleoclimate parameter data of each hydrocarbon source rock sample, and generating a TOC evolution chart based on the qualitative paleoclimate parameter data and the quantitative paleoclimate parameter data of each hydrocarbon source rock sample, thereby realizing intelligent paleoclimate reconstruction, reducing manpower resources consumed by paleoclimate reconstruction in related technologies, and improving the efficiency of paleoclimate reconstruction. And the paleoclimate reconstruction efficiency is improved.

The invention discloses a ludisia discolor crossbreeding method which comprises the following steps: spraying 50.0-80.0 mg / L of paclobutrazol in combination with a close planting treatment mode to delay the delayed flowering of an early flowering variety parent plant for 6-12 days, spraying 60.0-90.0 mg / L of 6-BA, adding 28.0-425.0 mg / L of KH2PO4 and 33.0-124.0 mg / L of H3BO33.0 into a matrix, and combining a thin planting treatment mode to promote the late flowering variety parent plant to flower 6-11 days in advance. Or the improved MS culture medium is combined with blue-red composite light to culture ludisia discolor plants in a culture room, the ludisia discolor plants are induced and cultured to bloom, transplanting is not needed, the ludisia discolor plants are directly cultured to bloom, the blooming period is prolonged, and supply of maternal plants in the pollination period is better guaranteed; according to the method, pollination is completed in a time window when ludisia discolor plant male and female parent pollen and stigma activity are optimal and the pollination state is good, the hybrid pollination rate is increased, sterile sowing of hybrid fruit pods is conducted through an improved Kundson C culture medium, germination can be conducted for 28 days, the germination rate is high, seedlings are strong, the development success rate of hybrid embryos is high, and new germplasm is provided for extraction or planting appreciation of special natural drugs.