228 results about "Transgenic lines" patented technology

Transgenic plants are more tolerant to environmental stresses than untransformed plants. The pea ABR17 (Abscisic acid responsive 17) is used to enhance germination of plants such as Arabidopsis sp. and Brassica sp. while under multiple abiotic stresses, and to enhance the tolerance of these plants to these stresses. Three independently derived Arabidopsis transgenic lines, containing ABR17, germinated better in the presence of salt, cold temperature or both. The transgenic plants also exhibited enhanced tolerance to freezing temperature or extreme heat. Furthermore, the transgenic plants demonstrated early flowering even under normal, non-stressed conditions.

A method of expressing in vivo heart-specific fluorescence in transgenic line of zebrafish is developed, which provides a research model for studying heart-related gene functions and performing gene therapies in the future. The method comprises the following steps. Firstly, a plasmid is constructed. This plasmid construct includes the upstream regulatory region, the exon 1, the intron 1, and the exon 2 of cmlc2 gene, cDNA of GFP, wherein the cmlc2 gene and GFP cDNA form a cassette, and inverted terminal repeats from adeno-associated virus are flanked at both sides of this cassette. The plasmid construct is linearized and microinjected into one-celled zebrafish fertilized eggs. Lastly, the heart-specific fluorescent expressed zebrafish are selected and the germline-transmitting transgenic strain is generated.

The invention discloses an application of an OsGRF6 protein in regulation of plant height. According to the application of the OsGRF6 protein in the regulation of the plant height provided by the invention, an amino acid sequence of the OsGRF6 protein is sequence 2 in a sequence table. In the above application, a nucleotide sequence of a coding gene of the OsGRF6 protein is sequence 1 in a sequence table. Experiments show that a gene OsGRF6 of GRF family is cloned in japonica rice (ZH10); and positive transgenic rice lines are obtained by transferring rice ZH10 callus by an agrobacterium-mediated method. By analyzing the transgenic lines and corresponding wild type phenotypes, obvious dwarf characteristics are found in the transgenic rice.

The invention relates to an exogenous insert vector flanking sequence for transgenic rice and application of the flanking sequence and belongs to the technical field of biology. According to the invention, a flanking sequence of an exogenous insert fragment of a transgenic rice variety SK-2 is cloned for the first time; the application that the flanking sequence of the exogenous insert fragment of the transgenic rice variety SK-2 can be used as a target DNA (Deoxyribose Nucleic Acid) amplification fragment is defined; and the variety specific qualitative PCR (Polymerase Chain Reaction) detection of a specific transgenic rice variety SK-2 is established. The cloned and separated flanking sequence of the exogenous insert fragment can be further used for establishing a transgenic rice variety SK-2 PCR detection method. The flanking sequence can be used for designing a specific primer for the amplification of target DNA and establishing the variety specific qualitative and quantitative PCR detection of the specific transgenic rice variety SK-2.

The invention relates to molecular biology, and particularly discloses a method for detection of transgenic crops.Inner primers and outer primers are designed according to the endogenous gene, exogenous gene and transgenic strain flanking sequence of a transgenic crop, all the inner primers and outer primers are mixed firstly for the first round of high-pass multi-enrichment quantitative PCR with a small cycle number (15 cycles), then the second round of fluorogenic quantitative PCR is conducted with the inner primers, a result is analyzed according to an amplification curve and a fusion curve, and in this way, the success rate and stability of the method are improved remarkably and the purpose of high-pass detection of transgenic crops can be realized.The sensitivity of the method is higher than that of ordinary fluorogenic quantitative PCR by about one magnitude order; the method has the same quantitative capacity as ordinary fluorogenic quantitative PCR, has high specificity and is a more effective method for detection of transgenic crops.

Provided is an application of an OsmiR156f gene in rice effective tillering increasing. The OsmiR156f gene which has important regulating effect on rice tillering development is selected from a miR156 family, a precursor pri-miR156f sequence is cloned, through a stem specific promoter vector construction method, the OsmiR156f gene which can increase tillering is guided into rice, specific expression in the stem of rice is carried out, and rice tillering development is regulated. The early tillering capacity of transgenic rice can be improved properly, tillering sprouts at a high section and later-period tillering are still restrained, accordingly, the effective tillering quantity of a single rice plant can be increased, ineffective tillering quantity is not increased, a transgenic line rice ear number is increased properly, influence on yield correlated characters such as rice ear length and thousand kernel weight is small, and finally the purpose of single-plant production increasing is achieved.

A process for configuring a transgenic line of early-mature grass includes such steps as sampling the stem tip of early-mature seedling, in vitro culturing to induce its gibbosity, taking its tissue, culturing to induce its bunch sprouts, reproducing the bunch sprouts rooting culture to obtain complete plant, and using gene gun to bombard the sprout tuber to obtain its transgenic plant.

The invention discloses a marker set and composition for comprehensively screening genetically modified ingredients and application. The marker set comprises seven screening elements including PCAMV35S, TCAMV35S, TNOS, PRbcS4, TPINII, TE9 and PAT; the marker set further comprises strain-specific sequences of corn DAS40278, a soybean DP305423 and a soybean CV127. The composition is a reagent for detecting the marker set, preferably primers and probes shown in SEQ ID No.1-33. The marker set and composition can cover 66 commercial genetically modified strains on the market, have the advantages ofgood repeatability, high throughput, sensitivity, accuracy and rapidity, and have good application prospects in the aspect of detecting the genetically modified components.

The invention discloses a construction method of a rice engineering maintainer line. The method comprises the following steps: 1, integrating an exogenous reporter gene to the chromosome of wild type rice in a site-specific integration manner to obtain a transgenic line containing the exogenous reporter gene, and then executing the step 2, wherein the exogenous reporter gene and a wild type fertility regulatory gene can be subjected to close linkage inheritance; 2, hybridizing the transgenic line obtained in the step 1 with a homozygous recessive rice common nuclear male sterile line, and screening a strain containing the exogenous reporter gene as the needed rice engineering maintainer line, wherein the homozygous recessive rice common nuclear male sterile line is a fertility regulatory gene mutant strain. The invention further discloses application of the rice engineering maintainer line to rice breeding. By integrating the exogenous reporter gene to the chromosome of the wild type rice in the site-specific integration manner, the problem of limitation on transformation of a receptor material is solved. The rice engineering maintainer line is available for rice common recessive nuclear male sterile line propagation.

The invention discloses a method for identifying heat resistance of capsicum by the utilization of in vitro leaf disk. The method mainly includes capsicum heatproof line B35 used as a heatproof contrast, capsicum heat-labile line B6 used as a temperature-sensitive contrast, heat resistance identification of capsicum in vitro leaf disk, heat damage grading standard of in vitro leaf disk and the like. The method is characterized in that when the capsicum in vitro leaf disk is processed at specific temperature (high temperature and normal temperature), at specific concentration of a salt solution and within a certain period of time, different heatproof lines have distinct differences in leaf disk phenotype symptom. On this account, through statistics and calculation of heat damage index of some line and according to the established heat damage grading standard, heat resistance lines and heat sensitivity lines of capsicum can be identified. The method is especially suitable for identification and genetic analysis of heat resistance of those rare and transgenic lines of capsicum material, is a method which is simple and rapid to operate and effectively identifies heat resistance of the capsicum germplasm resources, and can be adopted to accelerate the progress of capsicum heatproof breeding.