156 results about "Transgenic lines" patented technology

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention relates to a salt inducible promoter PagVQ5Pro and application thereof, and relates to the technical field of plant genetic engineering, and the nucleotide sequence of the promoter PagVQ5Pro is as shown in SEQ ID NO. 1. According to a GUS dyeing result, a transgenic line PagVQ5Pro-OE1, 3 for overexpressing the poplar promoter PagVQ5Pro can obviously enhance the GUS dyeing activity under a salt induction condition, so that the salt-induced expression of the poplar promoter PagVQ5Pro is proved, and a theoretical basis is laid for the application of the poplar promoter PagVQ5Pro in stress-resistant breeding.

The invention belongs to the field of molecular biology, and particularly relates to application of OsDOF3 protein or a coding gene thereof to improvement of rice broad-spectrum disease resistance and regulation of agronomic traits. According to the application, an OsDOF3 knockout mutant and an overexpression transgenic line are constructed under the background of susceptible rice, resistance phenotype analysis is performed by inoculating various pathogenic bacteria (including pyricularia oryzae, rhizoctonia solani and bacterial blight), and it is proved that OsDOF3 participates in regulation and control of an immunoreaction pathway of rice to fungal diseases (rice blast and sheath blight) and bacterial diseases (bacterial blight). Meanwhile, the application also finds that after a strong promoter ubi is adopted for driving, an overexpression material of the OsDOF3 is shorter than that of a wild plant, and the OsDOF3 influences normal growth and development of rice while stimulating plant immune response. The invention provides important gene resources and molecular theoretical basis for developing a new variety of broad-spectrum disease-resistant rice.

The invention relates to the technical field of plant genetic breeding, in particular to a rice sheath blight resistance related gene and an encoding protein and application thereof. The invention provides a rice sheath blight resistance related gene as well as an encoding protein and application thereof, the gene is OsSBR4 or a related gene thereof, the resistance of rice to sheath blight can be remarkably enhanced by knocking out the gene, and the resistance of rice to sheath blight can be reduced by overexpressing the gene. After agronomic traits of a transgenic line of the gene are investigated, it is found that the plant height, the ear length, the ear number, the thousand seed weight and the like are not affected, and it is indicated that the gene has important application value in sheath blight resistance molecular breeding. According to the invention, a novel rice sheath blight resistance related gene is screened and excavated, and the identification of the sheath blight resistance gene can provide a theory and a basis for breeding a sheath blight resistance rice variety.

The invention provides a CsERECTA gene-based tea tree leaf shape regulation and control method and a tea tree light utilization rate improving method, which are characterized in that a sequence of a tea tree gene is cloned to obtain a complete sequence; then, the CsERECTA is subjected to bioinformatics analysis, expression of a GUS reporter gene is started by constructing a plant expression vector and connecting a target fragment, and a recombinant plant vector is used for genetic transformation of tobacco, so that the function of the CsERECTA is verified; the character of the CsERECTA transgenic line is analyzed, the leaf shape is determined, the method capable of improving the leaf shape of the tea tree is obtained, and a foundation is laid for cultivating a new variety and improving the light utilization rate of the tea tree by using the CsERECTA gene.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

The invention belongs to the technical field of biology, and particularly relates to application of TaWDR3 protein and a coding gene thereof in regulating drought resistance of wheat. According to the application, a recombinant vector p110UBI-R of a TaWDR3 gene CDS sequence is constructed and a wheat plant is transformed, so that the survival rate of a T3-generation wheat transgenic line obtained through TaWDR3 overexpression under a drought condition is obviously higher than that of wild type wheat; the yield of a single plant is averagely increased by about 18%, and statistically, the TaWDR3 protein and the coding gene thereof have the function of positively regulating the drought resistance of the wheat, the TaWDR3 gene is over-expressed in the wheat, the growth state of the wheat under the drought stress condition can be improved, and the survival rate can be increased; the method has important practical significance for culturing new varieties of drought-resistant crops.

The invention discloses application of an MsMYB-ZD gene in regulating and controlling the leaf size and yield of medicago sativa, and belongs to the technical field of plant genetic engineering. According to the invention, an MsMYB-ZD transcription factor for regulating and controlling the leaf size of medicago sativa is cloned by using transcriptome analysis; experimental data show that the MsMYB-ZD gene presents a gradually up-regulated expression mode in a leaf development process; compared with wild alfalfa Gongnong No.1 1-16 single plants, the leaf area of the overexpressed MsMYB-ZD transgenic single plant is averagely enlarged by 77%, and the expression quantity and the leaf area of the transgenic line show a remarkable positive correlation relationship; the fresh grass yield of a transgenic single plant is increased by 158.7%, and the hay yield of a single plant is increased by 207.5%. The results show that the gene has important significance for improving the yield of medicago sativa.

The invention discloses an application of a millet SiPYL3 gene and an encoding protein thereof in regulation and control of plant stress resistance and / or yield. The invention relates to the field of plant genetic engineering, and provides application of SiPYL3 protein or a coding gene thereof in regulation and control of plant salt resistance and / or drought resistance and / or yield. The amino acid sequence of the SiPYL3 protein is as shown in SEQ ID No. 1. According to the invention, the coding gene of the SiPYL3 protein from millet is introduced into rice, so that the salt tolerance of the obtained transgenic line is improved, the drought resistance is reduced, and the yield is increased. Therefore, the SiPYL3 gene can positively regulate the salt tolerance and yield traits of the plant and negatively regulate the drought resistance of the plant. The invention has important significance for cultivating salt-resistant and high-yield plant varieties.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention belongs to the technical field of biotechnology, and relates to a wheat drought-resistant related protein TaELF4 as well as a coding gene and application thereof. A recombinant vector p110UBI-R containing a TaELF4 gene CDS sequence of SEQ ID NO.1 is used for transforming wheat, the survival rate of an obtained T3-generation transgenic line is remarkably higher than that of wild type wheat under the drought condition, the yield is averagely increased by about 15%, and the yield is statistically remarkably higher than that of the wild type wheat. The invention has important significance in molecular breeding for regulating and controlling the drought resistance and yield of wheat.

This invention discloses the application of the GhATG18a gene in accelerating callus induction in cotton. The invention constructs a pCAMBIA2300-GhATG18a overexpression vector from the full-length CDS fragment of this gene, then introduces it into Agrobacterium, and transforms cotton using Agrobacterium-mediated transformation to obtain transgenic lines. qRT-PCR results show that, compared with the CCRI24 control material, the expression level of the GhATG18a gene in the overexpression lines OE-GhATG18a-1 and OE-GhATG18a-2 is significantly upregulated. Phenotypic observation and histological sections both show that the OE-GhATG18a line overexpressing this gene accelerates the process of somatic cell dedifferentiation and callus induction in cotton, thereby improving the genetic transformation efficiency of cotton. Therefore, overexpressing this gene and applying it to commercial cotton varieties that are difficult to genetically transform can help accelerate the callus induction process, thereby improving the genetic transformation efficiency and providing technical support for breeding high-yield and stress-resistant high-quality cotton germplasm.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

The application discloses a use of a wheat TaCIPK19-3D gene and a method for improving salt tolerance of crops. The wheat TaCIPK19-3D gene is taken as the object, and it is found that wild type (WT), a transgenic line overexpressing the TaCIPK19-3D gene and an oscipk19 gene knockout mutant plant all show obvious phenotype differences after salt stress. The transgenic line overexpressing the TaCIPK19-3D gene shows obvious salt tolerance phenotype, and the leaves of the transgenic line overexpressing the TaCIPK19-3D gene are greener and more active than those of the wild type and the mutant after salt treatment. The leaves of the oscipk19 gene knockout mutant line of a rice homologous gene are more withered than those of the wild type after salt treatment. The results show that the TaCIPK19-3D gene can positively regulate the salt tolerance of plants, and can be applied to the breeding of wheat salt-tolerant varieties through molecular breeding and other technologies.

The invention relates to a salt induced expression promoter PagVQ1Pro and application thereof, and relates to the technical field of plant genetic engineering, and the nucleotide sequence of the promoter PagVQ1Pro is shown as SEQ ID NO.8. The invention also relates to a preparation method of the salt induced expression promoter PagVQ1Pro. A salt stress induction experiment is carried out on a poplar transgenic line PagVQ1Pro-OE4, 6 and 10 of the overexpression promoter PagVQ1Pro, it is known that under the salt induction condition, the GUS dyeing activity in leaves of the transgenic line of the overexpression poplar promoter PagVQ1Pro is remarkably enhanced, and the result shows that the promoter PagVQ1Pro is subjected to salt induction expression.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PaVAMP711-a protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-a protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-a gene. Through a gene editing technology, the PaVAMP711-a gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention belongs to the technical field of plant genetic engineering and biology, and particularly relates to application of a muskmelon CmEXPB1 gene to improvement of fruit crack resistance. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, the gene CmEXPB1 is cloned from muskmelon cDNA, and a real-time fluorescence quantification technology is utilized to find that the expression quantity of the gene in anti-cracking sweet melon peel is obviously higher than that of easily-cracking peel. A plant expression vector is constructed and transformed into tomatoes, and it is found that peel epidermis hardness, cortical layer toughness and compactness of a transgenic line are remarkably higher than those of a wild type, and brittleness is remarkably reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, so that the CmEXPB1 is proved to enhance the crack resistance by adjusting cell wall components. The invention lays a theoretical foundation for researching the fruit crack resistance, and also provides a gene resource for culturing horticultural plants with high-crack-resistance fruits.

The application discloses a red raspberry RuSA1 gene, an expression protein thereof and application, and relates to the technical field of plant genetic engineering.The red raspberry RuSA1 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein thereof is as shown in SEQ ID NO.2.The application constructs an expression vector of the red raspberry RuSA1 gene, and then transforms the expression vector into tomatoes;transgenic tomato plants with significantly increased anthocyanin content are obtained through cultivation and screening.The RuSA1 gene promotes the synthesis of anthocyanins in the fruits of the transgenic lines.The expression amounts of the genes SlABA, SlNCED1, SlACS2 and SlACO1 related to the secretion of ABA hormone, the gene SlIAA36 related to the secretion of IAA hormone, and the genes Sl2ox4 and Sl20x2 related to the secretion of GA hormone are all significantly higher than those of CK.

The invention discloses an application of an OsmiR396 / GRF-MAPK6 signal channel in regulation and control of rice grain shape and thousand grain weight, and particularly relates to an application of interaction of a target gene OsGRF4 (GS2) encoding protein of OsmiR396 and an OsMAPK6 gene encoding protein in regulation and control of rice grain shape and thousand grain weight. By hybridizing an OsGRF4 gene overexpressed transgenic line and an OsMAPK6 gene phosphorylated overexpressed transgenic plant, the grain shape of a progeny is further increased compared with that of a parent on the basis that the grain shape of the parent is increased, and the breeding method obtained based on the research has the effect of remarkably increasing the rice yield.

The invention provides application of an arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of plants. The nucleotide sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.1, and the CDS sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.3. The invention also provides application of the arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of the plants. The arabidopsis thaliana CPK12 gene is used for negative regulation of the lead stress tolerance of plants. According to the application, an arabidopsis thaliana CPK12 gene is adopted as a negative regulatory factor to participate in a new function of responding to lead stress of a plant, an RNA interference vector RNAi targeting the arabidopsis thaliana CPK12 gene is constructed and is transformed into wild-type arabidopsis thaliana by utilizing a genetic engineering technology, and a homozygous transgenic line with a silent CPK12 gene expression is obtained through resistance screening. Experiments prove that the resistance of a transgenic line to lead stress is remarkably enhanced, which indicates that the CPK12 gene plays a negative regulation role in a plant lead stress response pathway. The invention provides a new molecular target for regulating and controlling the heavy metal stress resistance of plants by using the CPK12 gene, and has important application value in the field of phytoremediation of heavy metal contaminated soil and in the aspect of molecular breeding of lead-resistant crop varieties.

The invention discloses application of a sorghum SbPGK1 gene in improving plant disease resistance, and belongs to the technical field of biology. The nucleotide sequence of the sorghum SbPGK1 gene is as shown in SEQ ID NO.9. By analyzing phenotypes and related physiological indexes of SbPGK1 rice transgenic lines before and after inoculation of different pathogenic bacteria for infection and sorghum, the results show that compared with non-SbPGK1 gene rice lines, the SbPGK1 transgenic lines have obviously better resistance to magnaporthe oryzae and bacterial blight germs than wild types, silence SbPGK1 in sorghum has better resistance to rice blast germs and bacterial blight germs, and the SbPGK1 gene has better resistance to rice blast germs and bacterial blight germs. The SbPGK1 gene has the advantages that the SbPGK1 silent strain has reduced disease resistance to colletotrichum gloeosporioides, the SbPGK1 is overexpressed in sorghum, and the SbPGK1 overexpressed strain has enhanced disease resistance to colletotrichum gloeosporioides, so that the SbPGK1 gene is a potential disease-resistant breeding gene and can be used for plant disease-resistant genetic improvement.

The invention relates to application of an StEtu1 gene in promoting tuberization of potatoes and cultivating early-maturing varieties of potatoes, and belongs to the technical field of gene engineering. In order to excavate genes capable of promoting early tufting of potatoes and further cultivate early-maturing varieties of the potatoes, the day and night expression modes of the StEtu1 genes of the potatoes are analyzed, and it is found that the expression modes of the StEtu1 genes are basically the same under the long-day or short-day condition; the potato StEtu1 gene is successfully cloned, and by constructing a StEtu1 gene overexpression transgenic line, it is found for the first time that the potato StEtu1 gene can promote early tuturization of potatoes, and then the potato StEtu1 gene can be used for cultivation of early-maturing varieties of potatoes. Theoretical support is provided for rapid cultivation of new varieties of breakthrough precocious potatoes, and the method has great and profound strategic significance in optimization of potato variety structures in China, breakthrough of planting area limitation, balance of market supply and improvement of overall benefits of an industrial chain.

The invention belongs to the technical field of molecular biotechnology and plant genetic engineering, and particularly relates to a grape MYB transcription factor VvMYB108 and application thereof. The invention provides an MYB transcription factor VvMYB108 for regulating and controlling biological stress in a postharvest storage process of grapes. An overexpression strain of the VvMYB108 in arabidopsis thaliana is utilized to find that the sensitivity of a VvMYB108 transgenic strain to botrytis cinerea is enhanced. By using an overexpression strain of the VvMYB108 in arabidopsis thaliana, it is found that the disease resistance of the VvMYB108 transgenic strain to Pst DC3000 is enhanced.

The invention discloses an application of a silkworm silk glue protein sericin 4 repetitive motif in improvement of silk and sericin hydrogel performance and a method thereof, a second repetitive region of a sericin 4 gene is completely expressed in a silkworm cocoon sericin layer through cloning and micro-injection, a bred transgenic strain improves the silk mechanical property, the sericin hydrogel performance is remarkably improved, and the sericin protein sericin 4 repetitive motif can be used as a sericin protein sericin 4 repetitive motif. The material is expected to be applied to biological materials and tissue medical engineering.

The invention discloses a gingko GbC4H1 gene as well as an encoding protein and application thereof, and relates to the technical field of plant genetic engineering. The nucleotide sequence of the gingko GbC4H1 gene disclosed by the invention is as shown in SEQ ID NO. 1, and the amino acid sequence of the encoded protein of the gingko GbC4H1 gene is as shown in SEQ ID NO. 2. The method comprises the following steps: constructing an expression vector of the gingko GbC4H1 gene, and transforming the expression vector into the nicotiana benthamiana; and cultivating and screening to obtain a transgenic plant with increased flavonoid metabolic substance content. Compared with a control group, the expression level of regulation and control transcription factors of flavone synthesis pathway related genes and flavone synthesis pathway structural genes in a transgenic line is remarkably improved. The content of flavonoid metabolites in a transgenic strain is increased, and compared with a control group, 14 kinds of differential metabolites are up-regulated and 20 kinds of differential metabolites are down-regulated.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.