125 results about "Transgenic lines" patented technology

The invention discloses application of a GsRZ1A gene in improving alkali resistance of plants, and belongs to the technical field of genetic engineering. The nucleotide sequence of the GsRZ1A gene is as shown in SEQ ID NO: 1, and the coded amino acid sequence of the GsRZ1A gene is as shown in SEQ ID NO: 2. A transgenic line is obtained by constructing an overexpression vector of the GsRZ1A gene and transforming soybeans. A transgenic line is subjected to alkali stress treatment, and a result shows that the overexpressed GsRZ1A soybean line shows a faster relative growth rate, the total leaf area, the leaf chlorophyll content and the relative water content are all higher than those of a control line of an empty vector, the total root length and the total root surface area of a root system are also remarkably larger than those of a control group, and the antioxidant enzyme activity can be improved; the accumulation of superoxide anions and malonaldehyde is reduced, and the accumulation of osmotic regulation substances such as soluble sugar is increased to enhance the tolerance of the soybeans to alkali stress, so that the method has an important application prospect in alkali-resistant plant breeding.

The invention provides a CsERECTA gene-based tea tree leaf shape regulation and control method and a tea tree light utilization rate improving method, which are characterized in that a sequence of a tea tree gene is cloned to obtain a complete sequence; then, the CsERECTA is subjected to bioinformatics analysis, expression of a GUS reporter gene is started by constructing a plant expression vector and connecting a target fragment, and a recombinant plant vector is used for genetic transformation of tobacco, so that the function of the CsERECTA is verified; the character of the CsERECTA transgenic line is analyzed, the leaf shape is determined, the method capable of improving the leaf shape of the tea tree is obtained, and a foundation is laid for cultivating a new variety and improving the light utilization rate of the tea tree by using the CsERECTA gene.

The invention discloses application of a TpGST gene in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. According to the application of the TpGST gene disclosed by the invention in regulating and controlling the ear rot and stem rot resistance of maize fusarium graminearum, the nucleotide sequence of the TpGST gene is shown as SEQ ID NO. 1. Fusarium graminearum is inoculated to ears and stems of an overexpressed TpGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic line is improved; fusarium graminearum is inoculated to ears and stems of the corn hybrid agricultural large 3138 overexpression TpGST transgenic line, and the ear rot and stem rot resistance of the fusarium graminearum of the corn transgenic hybrid is improved.

The invention relates to the field of plant molecular biology and forest genetic engineering, and particularly provides a PagRAP2.3 protein point mutant and application thereof in drought resistance of poplar. The mutant PagRAP2.3 MA is obtained by mutating methionine at the first site and cysteine at the second site of a wild type PagRAP2.3 protein into methionine and alanine. The invention further discloses a preparation method of the mutant PagRAP2.3. An expression vector containing the PagRAP2.3 MA gene is constructed, poplar 84K is transformed through an agrobacterium-mediated method, and a transgenic line with stable expression is obtained. Functional verification results show that overexpression of PagRAP2.3 MA can significantly increase the plant height of the poplar and promote plant growth, but the sensitivity to moisture is enhanced under drought stress. The mutant can be used for regulating and controlling the growth and development of forest trees and evaluating the drought resistance, and has a forestry breeding application prospect.

The invention discloses application of an MsMYB-ZD gene in regulating and controlling the leaf size and yield of medicago sativa, and belongs to the technical field of plant genetic engineering. According to the invention, an MsMYB-ZD transcription factor for regulating and controlling the leaf size of medicago sativa is cloned by using transcriptome analysis; experimental data show that the MsMYB-ZD gene presents a gradually up-regulated expression mode in a leaf development process; compared with wild alfalfa Gongnong No.1 1-16 single plants, the leaf area of the overexpressed MsMYB-ZD transgenic single plant is averagely enlarged by 77%, and the expression quantity and the leaf area of the transgenic line show a remarkable positive correlation relationship; the fresh grass yield of a transgenic single plant is increased by 158.7%, and the hay yield of a single plant is increased by 207.5%. The results show that the gene has important significance for improving the yield of medicago sativa.

The invention belongs to the technical field of plant breeding in molecular biology, and provides a cotton GhVIP1 gene and application thereof in drought resistance and salt tolerance of plants. The nucleotide sequence of the GhVIP1 gene is as shown in SEQ ID NO: 1, and the amino acid sequence of the GhVIP1 gene is as shown in SEQ ID NO: 2. The gene is obviously up-regulated under the induction of salt and drought stress. An arabidopsis thaliana homozygous line overexpressed with the GhVIP1 gene is obtained through a transgenic technology, under the stress of drought and salt, the seed germination rate of the arabidopsis thaliana overexpressed with the gene is higher than that of a wild type, the growth condition of a transgenic line in the seedling stage is better than that of the wild type, the stem length, the total antioxidant capacity and the chlorophyll content are all superior to those of the wild type, and the MDA content is lower than that of the wild type; further, the drought resistance and the salt resistance of the arabidopsis thaliana are improved. When the gene is applied to drought-resistant and salt-resistant breeding of plants, the drought resistance and salt resistance of the plants can be improved, and an effective alternative scheme is provided for molecular breeding of drought-resistant and salt-resistant crops.

The invention belongs to the technical field of biotechnology, and relates to a wheat drought-resistant related protein TaELF4 as well as a coding gene and application thereof. A recombinant vector p110UBI-R containing a TaELF4 gene CDS sequence of SEQ ID NO.1 is used for transforming wheat, the survival rate of an obtained T3-generation transgenic line is remarkably higher than that of wild type wheat under the drought condition, the yield is averagely increased by about 15%, and the yield is statistically remarkably higher than that of the wild type wheat. The invention has important significance in molecular breeding for regulating and controlling the drought resistance and yield of wheat.

This invention discloses the application of the GhATG18a gene in accelerating callus induction in cotton. The invention constructs a pCAMBIA2300-GhATG18a overexpression vector from the full-length CDS fragment of this gene, then introduces it into Agrobacterium, and transforms cotton using Agrobacterium-mediated transformation to obtain transgenic lines. qRT-PCR results show that, compared with the CCRI24 control material, the expression level of the GhATG18a gene in the overexpression lines OE-GhATG18a-1 and OE-GhATG18a-2 is significantly upregulated. Phenotypic observation and histological sections both show that the OE-GhATG18a line overexpressing this gene accelerates the process of somatic cell dedifferentiation and callus induction in cotton, thereby improving the genetic transformation efficiency of cotton. Therefore, overexpressing this gene and applying it to commercial cotton varieties that are difficult to genetically transform can help accelerate the callus induction process, thereby improving the genetic transformation efficiency and providing technical support for breeding high-yield and stress-resistant high-quality cotton germplasm.

The application of a soybean GmGASA1 gene, the application relates to the application of a gibberellin regulation related protein GmGASA1 coding gene of soybean.The application of the soybean GmGASA1 gene, namely the application of improving the protein content, the seed amino acid content and the grain weight of plants; the application of the soybean GmGASA1 gene in plant breeding; the application of the soybean GmGASA1 gene in cultivating transgenic plants.The experimental results show that three transgenic lines are obtained by overexpressing the GmGASA1 gene in soybean, the protein content and the grain weight of the transformed soybean seeds are significantly improved compared with the seeds of the untransformed receptor plants, which indicates that the GmGASA1 gene can regulate the protein content, the seed amino acid content and the grain weight of seeds.The GmGASA1 gene can be used for improving the quality of soybean and increasing the yield of soybean.The application of the GmGASA1 gene can effectively realize the breeding of new soybean varieties and the creation of new germplasm.

The invention discloses application of a glutathione transferase gene EuGST in regulating and controlling ear rot and stem rot resistance of maize fusarium graminearum, and belongs to the technical field of gene engineering. The invention discloses an application of a glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. A nucleotide sequence of the glutathione transferase gene EuGST is shown as SEQ ID NO.1. The invention also discloses an application of the glutathione transferase gene EuGST in regulation and control of corn fusarium graminearum ear rot and stem rot resistance. Fusarium graminearum is inoculated to ears and stems of an overexpressed EuGST transgenic line, so that the ear rot and stem rot resistance of the fusarium graminearum of the maize inbred line is improved; fusarium graminearum is inoculated to ears and stems of the overexpressed EuGST transgenic hybrid, so that the ear rot and stem rot resistance of the fusarium graminearum of the corn hybrid is improved.

The application discloses a use of a wheat TaCIPK19-3D gene and a method for improving salt tolerance of crops. The wheat TaCIPK19-3D gene is taken as the object, and it is found that wild type (WT), a transgenic line overexpressing the TaCIPK19-3D gene and an oscipk19 gene knockout mutant plant all show obvious phenotype differences after salt stress. The transgenic line overexpressing the TaCIPK19-3D gene shows obvious salt tolerance phenotype, and the leaves of the transgenic line overexpressing the TaCIPK19-3D gene are greener and more active than those of the wild type and the mutant after salt treatment. The leaves of the oscipk19 gene knockout mutant line of a rice homologous gene are more withered than those of the wild type after salt treatment. The results show that the TaCIPK19-3D gene can positively regulate the salt tolerance of plants, and can be applied to the breeding of wheat salt-tolerant varieties through molecular breeding and other technologies.

The invention belongs to the technical field of gene engineering, and particularly relates to application of PaVAMP711-a protein in regulating and controlling potassium stress resistance of plants. The PaVAMP711-a protein disclosed by the invention is a vesicle transport related protein and belongs to a class of SNARE proteins, and the potassium stress resistance of plants can be improved by knocking out the PaVAMP711-a gene. Through a gene editing technology, the PaVAMP711-a gene in the poplar is knocked out, two obtained transgenic lines have high potassium stress resistance phenotypes, and the fresh weight and the plant height of the transgenic lines are remarkably increased compared with those of wild types. Theoretical basis and technical support are provided for reasonable fertilization, soil improvement and cultivation of high-potassium-stress-resistant forest tree varieties in production, so that the growth performance and yield of crops in an adverse soil environment are improved, and sustainable development of forestry is guaranteed.

The invention discloses a wheat stripe rust adult plant resistance gene Yr54, a specific functional molecular marker and application of the wheat stripe rust adult plant resistance gene Yr54 in wheat stripe rust resistance breeding, and belongs to the technical field of biology. A wheat stripe rust adult-plant resistance gene TaNLR2DL transgenic line is subjected to adult-plant-stage stripe rust resistance identification, and a result shows that compared with a non-TaNLR2DL gene line, the stripe rust resistance of the TaNLR2DL transgenic line is obviously improved, while a TaNLR2DL gene knockout line is contrary to the TaNLR2DL gene knockout line, so that the TaNLR2DL is a potential stripe rust resistance gene, and the TaNLR2DL gene knockout line can be used for identifying the stripe rust resistance in the adult-plant stage. The method can be used for genetic improvement of wheat stripe rust resistance. The invention further develops a functional specific molecular marker of TaNLR2DL, and the marker can quickly screen wheat germplasm containing TaNLR2DL, so that the time cost is effectively saved, and the progress of wheat resistance germplasm genetic background detection and wheat disease-resistant gene-assisted selective breeding is accelerated.

The invention belongs to the technical field of plant genetic engineering and biology, and particularly relates to application of a muskmelon CmEXPB1 gene to improvement of fruit crack resistance. The nucleotide sequence of the gene is as shown in SEQ ID NO. 1. According to the invention, the gene CmEXPB1 is cloned from muskmelon cDNA, and a real-time fluorescence quantification technology is utilized to find that the expression quantity of the gene in anti-cracking sweet melon peel is obviously higher than that of easily-cracking peel. A plant expression vector is constructed and transformed into tomatoes, and it is found that peel epidermis hardness, cortical layer toughness and compactness of a transgenic line are remarkably higher than those of a wild type, and brittleness is remarkably reduced; cell wall component determination finds that the contents of cellulose, hemicellulose and protopectin of the transgenic tomato are increased, and the contents of soluble pectin and lignin are reduced, so that the CmEXPB1 is proved to enhance the crack resistance by adjusting cell wall components. The invention lays a theoretical foundation for researching the fruit crack resistance, and also provides a gene resource for culturing horticultural plants with high-crack-resistance fruits.

The application discloses a red raspberry RuSA1 gene, an expression protein thereof and application, and relates to the technical field of plant genetic engineering.The red raspberry RuSA1 gene disclosed by the application has a nucleotide sequence as shown in SEQ ID NO.1, and the amino acid sequence of the expression protein thereof is as shown in SEQ ID NO.2.The application constructs an expression vector of the red raspberry RuSA1 gene, and then transforms the expression vector into tomatoes;transgenic tomato plants with significantly increased anthocyanin content are obtained through cultivation and screening.The RuSA1 gene promotes the synthesis of anthocyanins in the fruits of the transgenic lines.The expression amounts of the genes SlABA, SlNCED1, SlACS2 and SlACO1 related to the secretion of ABA hormone, the gene SlIAA36 related to the secretion of IAA hormone, and the genes Sl2ox4 and Sl20x2 related to the secretion of GA hormone are all significantly higher than those of CK.

The invention provides application of an arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of plants. The nucleotide sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.1, and the CDS sequence of the arabidopsis thaliana CPK12 gene is as shown in SEQ ID NO.3. The invention also provides application of the arabidopsis thaliana CPK12 gene in improvement of lead stress resistance of the plants. The arabidopsis thaliana CPK12 gene is used for negative regulation of the lead stress tolerance of plants. According to the application, an arabidopsis thaliana CPK12 gene is adopted as a negative regulatory factor to participate in a new function of responding to lead stress of a plant, an RNA interference vector RNAi targeting the arabidopsis thaliana CPK12 gene is constructed and is transformed into wild-type arabidopsis thaliana by utilizing a genetic engineering technology, and a homozygous transgenic line with a silent CPK12 gene expression is obtained through resistance screening. Experiments prove that the resistance of a transgenic line to lead stress is remarkably enhanced, which indicates that the CPK12 gene plays a negative regulation role in a plant lead stress response pathway. The invention provides a new molecular target for regulating and controlling the heavy metal stress resistance of plants by using the CPK12 gene, and has important application value in the field of phytoremediation of heavy metal contaminated soil and in the aspect of molecular breeding of lead-resistant crop varieties.

The invention relates to application of an StEtu1 gene in promoting tuberization of potatoes and cultivating early-maturing varieties of potatoes, and belongs to the technical field of gene engineering. In order to excavate genes capable of promoting early tufting of potatoes and further cultivate early-maturing varieties of the potatoes, the day and night expression modes of the StEtu1 genes of the potatoes are analyzed, and it is found that the expression modes of the StEtu1 genes are basically the same under the long-day or short-day condition; the potato StEtu1 gene is successfully cloned, and by constructing a StEtu1 gene overexpression transgenic line, it is found for the first time that the potato StEtu1 gene can promote early tuturization of potatoes, and then the potato StEtu1 gene can be used for cultivation of early-maturing varieties of potatoes. Theoretical support is provided for rapid cultivation of new varieties of breakthrough precocious potatoes, and the method has great and profound strategic significance in optimization of potato variety structures in China, breakthrough of planting area limitation, balance of market supply and improvement of overall benefits of an industrial chain.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

The invention discloses an anti-clubroot gene BolC6. MYB122 and application thereof, and relates to the technical field of plant biology. The NDA sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 1, and the CDS sequence of the BolC6. MYB122 provided by the invention is as shown in SEQ ID NO. 2. A constructed 35S: BolC6. MYB122-eGFP overexpression vector is transformed into wild type arabidopsis thaliana, an arabidopsis thaliana myb122 mutant is obtained, namely an overexpression transgenic line is obtained, the resistance of overexpression positive regulation of the BolC6. MYB122 gene to plasmodiophora brassicae is clarified through disease resistance identification, and the transgenic line is a transgenic line. And a theoretical basis and gene resources are provided for clubroot-resistant molecular breeding of plants such as brassica napus.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

This invention discloses a ribonuclease derived from tartary buckwheat. FtRNH Genes, their expression vectors, and applications. This invention isolates ribonuclease from tartary buckwheat through gene cloning. FtRNH The gene, whose CDS sequence is shown in SEQ ID No. 1, and whose encoded protein amino acid sequence is shown in SEQ ID No. 2, is described in this invention. FtRNH Transgenic lines were obtained by genetic transformation into cultivated tartary buckwheat (ZK3) and subjected to ultraviolet stress treatment. Compared with wild-type materials, the transgenic lines showed increased expression. FtRNH The positive lines of tartary buckwheat exhibited stronger tolerance to ultraviolet radiation and less accumulation of UV-B-induced R-loops and cyclobutanepyrimidine dimers. This invention has significant application prospects in improving the tolerance of cultivated tartary buckwheat to ultraviolet stress or in breeding tartary buckwheat varieties resistant to ultraviolet stress.

The invention relates to the technical field of gene engineering, in particular to application of an AaTPS gene in regulation and control of plant seed sizes. The invention provides an application of an AaTPS gene in regulating and controlling the size of a plant seed. According to the invention, the wormwood AaTPS gene is cloned from Artemisia vulgaris, and an overexpression vector driven by a 35S constitutive promoter is successfully constructed and transformed into arabidopsis thaliana. Compared with a wild control plant, the expression quantity of the three transgenic Arabidopsis thaliana T3-generation strains is obviously increased. Compared with a wild type control, the thousand seed weights of the three transgenic arabidopsis pure line seeds are increased by 40.58%, 46.12% and 53.74% respectively and reach a remarkable level; meanwhile, the length of the seeds of the transgenic line is obviously increased and reaches a remarkable level, and the width of the seeds of the OE7 line is also obviously increased. The invention can provide a new gene resource for improving the size of plant seeds, and has important application value in the aspect of plant breeding.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

StEtu1 The application of genes in promoting potato tuber formation and breeding early-maturing potato varieties belongs to the field of genetic engineering technology. To discover genes that can promote early tuber formation in potatoes and thus breed early-maturing potato varieties, this invention involves the use of genes related to potatoes... StEtu1 Analysis of gene expression patterns during the day and night revealed that, regardless of whether the day or night conditions were long-day or short-day, StEtu1 The gene expression patterns are basically the same; then the potato was successfully cloned. StEtu1 Genes, through construction StEtu1 Gene overexpression transgenic lines were discovered for the first time in potatoes. StEtu1 The gene can promote early tuber formation in potatoes, which can then be used to breed early-maturing potato varieties. This invention provides theoretical support for the rapid breeding of breakthrough early-maturing potato varieties, and has significant and far-reaching strategic importance for optimizing my country's potato variety structure, overcoming planting area limitations, balancing market supply, and improving the overall efficiency of the industry chain.

The application belongs to the field of plant genetic engineering, and specifically discloses application of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance. The application constructs AnWRKY38 overexpression vector, and transforms Arabidopsis thaliana to obtain a transgenic line. Experimental results show that overexpression of AnWRKY38 gene can significantly improve the survival rate of plants under waterlogging stress, enhance photosynthetic activity, cell membrane integrity, antioxidant capacity and proline accumulation, and reduce malondialdehyde and hydrogen peroxide content. The application first excavates the new function of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance, provides gene resources and effective technical means for plant waterlogging tolerance breeding, and can be used for waterlogging resistance genetic improvement of forest trees and ecological restoration plants, and has important application prospect.

The invention belongs to the technical field of bioengineering and analytical chemistry, and provides a probe eZinCh-2 modified plasmid based on FRET (Fluorescence Resonance Energy Transfer) and application of the probe eZinCh-2 modified plasmid in zinc ion detection. According to the invention, the plasmid is constructed through molecular cloning and homologous recombination technologies, and is microinjected into a zebrafish embryo, so that a transgenic strain capable of stably inheriting and expressing the probe protein is successfully obtained; as a living body biological tool, the zebrafish of the strain is combined with a confocal microscope spectrum FRET technology, and real-time and quantitative monitoring and visual imaging of dynamic changes of zinc ions at living body, cell and tissue levels are achieved. The method solves the problem that real-time dynamic visual monitoring of the zinc ions cannot be realized in a living body model in the prior art, and is suitable for real-time microscopic quantitative analysis of the content of the zinc ions in model organisms such as zebra fish and the like.

The invention discloses application of an MdVQ22 gene in salt-tolerant genetic improvement of apples, and relates to the technical field of plant genetic engineering. The key salt stress response gene MdVQ22 is identified, and the salt tolerance of apple plants can be improved by silencing the gene. The analysis of phenotypes and related physiological indexes of the MdVQ22 transgenic line before and after salt treatment shows that compared with a non-transgenic line, MdVQ22 overexpression transgenic apple callus has obvious salt sensitivity, and compared with a contrast, a silent line of a silent MdVQ22 gene has an obvious salt tolerance advantage; therefore, the MdVQ22 gene is a potential salt-tolerant breeding gene and can be used for salt-tolerant genetic improvement of plants. The invention provides a new gene resource and technical path for cultivating salt-tolerant apple varieties, can promote the development of the apple planting industry in saline-alkali soil, and breaks through the limitation of land salinization on the high-quality development of the apple industry.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

The application discloses a plant coding gene and protein with improved cadmium resistance and promoted biomass and a transgenic strain. The application carries out RNA-seq sequencing on switchgrass under cadmium stress treatment, screens a differential expression gene with the highest up-regulation fold, and genome annotation shows that the gene codes a member of a heat shock protein 70 family (Hsp70), a chaperone BiP (Binding Protein) protein, and the gene is named as PvBiP2. An overexpression vector of the gene is constructed and is transferred into a cadmium-sensitive yeast mutant strain (ycf1), and the growth condition of the transgenic yeast on a cadmium-containing culture medium is obviously better than that of a control group. Overexpression of the PvBiP2 gene in switchgrass is found to significantly improve the cadmium resistance of the transgenic switchgrass and promote the biomass of the switchgrass.