22 results about "Transgenic lines" patented technology

The application discloses an Arabidopsis thaliana shade-avoidance regulation microprotein gene AtRAD-Like, an encoded protein and application thereof, relates to the technical field of genetic engineering, and the nucleotide sequence of the gene AtRAD-Like is shown in SEQ ID NO.1; and the amino acid sequence of the protein encoded by the gene AtRAD-Like is shown in SEQ ID NO.2. The application is characterized in that the Bar marker gene is used to impart herbicide resistance to a transformation receptor for screening and Western Blot is used to detect the expression of the AtRAD-Like-4MYC fusion protein, so that positive seedlings can be accurately and quickly detected, and phenotype analysis is further conducted on the AtRAD-Like gene overexpression transgenic line, it is found that the AtRAD-Like gene has a negative regulation effect on the hypocotyl length of plants in a shade environment, the finding provides a research basis for developing a new shade-tolerant plant, and has important significance.

The application discloses a use of a wheat TaCIPK19-3D gene and a method for improving salt tolerance of crops. The wheat TaCIPK19-3D gene is taken as the object, and it is found that wild type (WT), a transgenic line overexpressing the TaCIPK19-3D gene and an oscipk19 gene knockout mutant plant all show obvious phenotype differences after salt stress. The transgenic line overexpressing the TaCIPK19-3D gene shows obvious salt tolerance phenotype, and the leaves of the transgenic line overexpressing the TaCIPK19-3D gene are greener and more active than those of the wild type and the mutant after salt treatment. The leaves of the oscipk19 gene knockout mutant line of a rice homologous gene are more withered than those of the wild type after salt treatment. The results show that the TaCIPK19-3D gene can positively regulate the salt tolerance of plants, and can be applied to the breeding of wheat salt-tolerant varieties through molecular breeding and other technologies.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

Recombinant DNA molecules and constructs are provided that are useful for modulating gene expression in plants. One or more expression cassette(s) of a recombinant DNA molecule or construct may be excised from transgenic plants following transformation by the presence of flanking site-specific recombination sites in the recombinant DNA molecule or construct by expression of a recombinase enzyme encoded by the recombinant DNA molecule or construct. Such a recombinase system may be used to remove such expression cassette(s) from plants transformed with the recombinant DNA construct or vector. The recombinase transgene may be operably linked to a tissue-preferred or tissue-specific promoter for autoexcision in transformed plants without crossing to a different transgenic line expressing the recombinase. Methods for causing autoexcision of one or more expression cassette(s) in a transgenic plant, and plants and cells containing or transformed with a recombinant DNA molecule or construct of the present disclosure, are also provided.

This invention discloses a ribonuclease derived from tartary buckwheat. FtRNH Genes, their expression vectors, and applications. This invention isolates ribonuclease from tartary buckwheat through gene cloning. FtRNH The gene, whose CDS sequence is shown in SEQ ID No. 1, and whose encoded protein amino acid sequence is shown in SEQ ID No. 2, is described in this invention. FtRNH Transgenic lines were obtained by genetic transformation into cultivated tartary buckwheat (ZK3) and subjected to ultraviolet stress treatment. Compared with wild-type materials, the transgenic lines showed increased expression. FtRNH The positive lines of tartary buckwheat exhibited stronger tolerance to ultraviolet radiation and less accumulation of UV-B-induced R-loops and cyclobutanepyrimidine dimers. This invention has significant application prospects in improving the tolerance of cultivated tartary buckwheat to ultraviolet stress or in breeding tartary buckwheat varieties resistant to ultraviolet stress.

The application belongs to the field of plant genetic engineering, and specifically discloses application of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance. The application constructs AnWRKY38 overexpression vector, and transforms Arabidopsis thaliana to obtain a transgenic line. Experimental results show that overexpression of AnWRKY38 gene can significantly improve the survival rate of plants under waterlogging stress, enhance photosynthetic activity, cell membrane integrity, antioxidant capacity and proline accumulation, and reduce malondialdehyde and hydrogen peroxide content. The application first excavates the new function of Anemiospermum neriiforme AnWRKY38 gene in improving plant waterlogging stress resistance, provides gene resources and effective technical means for plant waterlogging tolerance breeding, and can be used for waterlogging resistance genetic improvement of forest trees and ecological restoration plants, and has important application prospect.

This invention discloses the artificial synthesis of the disease-resistant gene NLP27 and its application, belonging to the field of plant genetic engineering technology. This invention selects a conserved 27-amino acid fragment from Verticillium dahliae NLP1, modifies its nucleotide sequence, and synthesizes the NLP27 gene, which is then overexpressed in potato plants through genetic transformation. Inoculation analysis shows that transgenic plants with the NLP27 gene exhibit high resistance to potato late blight and scab. Quantitative PCR analysis shows that excessively high NLP27 transcript levels significantly inhibit potato plant growth. After screening for expression levels and disease resistance, three transgenic lines with moderate NLP27 expression levels (such as PT1, PT6, and PT7) were obtained. These lines significantly enhanced resistance to potato late blight and scab without sacrificing yield.

This invention discloses a tomato thioesterase gene. SlFATB2 and its application in regulating tomato growth, development, and disease resistance, the aforementioned SlFATB2 The gene has the nucleotide sequence shown in SEQ ID No. 1. Its CDS sequence was cloned using bioinformatics combined with molecular biology techniques, and a strong promoter was constructed. SlFATB2 Overexpression vectors were used to obtain transgenic tomato plants overexpressing the vectors. The CRISPR / Cas9 knockout system was used to obtain... SlFATB2 Gene knockout tomato transgenic lines. Experiments have confirmed that knockout... SlFATB2 This leads to pollen shrinkage, fruit and seed shrinkage, and susceptibility to gray mold; overexpression SlFATB2 It can enhance resistance to gray mold without affecting normal growth. This invention clarifies that... SlFATB2 The gene function can be precisely improved in tomato agronomic traits and disease resistance through gene editing or overexpression, and its application prospects are broad.

The present invention relates to the technical field of plant genetic engineering, and specifically relates to a method for improving the salt tolerance of soybeans. Provided is a GmZF392 protein capable of significantly improving the salt tolerance of a plant. The GmZF392 protein can not only effectively improve the salt tolerance of the plant, but also significantly increase the oil and fatty acid content of plant seeds. The oil and fatty acid content in the seeds of a constructed transgenic line overexpressing GmZF392 is significantly increased. Moreover, the salt tolerance of the transgenic line is significantly higher than that of a control line.

The application belongs to the technical field of biology, and discloses a transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application. The CDS sequence of the transcription factor SlNAC078 is shown as SEQ ID NO. 1, the transcription factor SlNAC078 can positively regulate flavonoid synthesis of tomatoes, overexpression of the gene can activate the promoters of SlCHS1, SlCHS2 and SlFLS genes, and significantly improve the flavonoid content in fruits; and the transcription factor SlNAC078 can also improve the root dry weight and single fruit weight of tomato plants, and realize the synergistic improvement of nutritional quality and yield. The transcription factor SlNAC078 for regulating flavonoid synthesis of tomatoes and application directly targets the regulation mechanism of key genes for flavonoid synthesis, the cultivation method is simple, the obtained transgenic line is genetically stable, and the transcription factor SlNAC078 provides a key gene target and technical scheme for tomato breeding, and has an important application prospect in the industrialized production of high-nutrition and high-yield tomato products.

This invention relates to the field of biotechnology, specifically to... StNF‑ YA9 Application of genes in improving drought resistance in potatoes. This invention involves constructing... StNF-YA9 Gene overexpression vectors, screening and identification of transgenic lines, and improvement of drought stress resistance were used to discover overexpression... StNF-YA9 The gene can significantly enhance the drought resistance of potato plants, providing core functional genes and breeding materials for molecular breeding of drought-resistant potatoes. It can solve the technical problems of poor drought resistance and unstable yield in arid areas of the current main potato varieties, and has important theoretical research value and industrial application prospects.

This invention belongs to the field of gene breeding technology, specifically relating to the application of the cascade signal kinase OsMAPKKK5 in improving rice resistance to bacterial blight. This invention provides the protein OsMAPKKK5 or substances regulating the activity and / or content of said protein, and the application of related biological materials. This invention constructs... OsMAPKKK5 Transgenic lines overexpressing the pathogen were inoculated with bacterial blight pathogens in the field for resistance phenotypic analysis, and the results showed that... OsMAPKKK5 Compared to wild-type rice varieties, the overexpressed transgenic lines showed shorter lesions and exhibited a resistance phenotype against bacterial blight, thus demonstrating improved immunity. OsMAPKKK5 The expression level of this gene can promote the resistance to bacterial blight in rice. This invention provides important genetic resources and molecular theoretical basis for the development of new rice varieties resistant to bacterial blight.

This invention discloses proteins and related biological materials, as well as the application of these proteins, or substances regulating the expression of the protein-coding gene, or substances regulating the activity or content of the protein, in regulating plant seed size and / or seed weight; the protein is a protein with the amino acid sequence SEQ ID No. 1. Knockout experiments based on the CRISPR-Cas9 system successfully obtained knockout transgenic lines of this protein-coding gene. Phenotypic identification and comparison of seeds obtained from self-pollination of transgenic positive plants with wild-type results showed that knocking out the target gene increased the seed size and / or seed weight of maize inbred lines; while overexpression of the protein-coding gene in Arabidopsis resulted in smaller and lighter seeds, thus demonstrating that gene Zm00001d025992 has an important biological function in seed size and seed weight phenotypes.

The application discloses a rice stigma width regulation gene qSTB-8 and application of an encoded protein thereof, a nucleotide sequence of the rice stigma width regulation gene qSTB-8 is shown as SEQ ID NO. 1, and the application is selected from any one or two or more of the following: (1) application in regulation of rice stigma width; (2) application in improvement of rice stigma exposure rate breeding; (3) application in improvement of rice cross-pollination rate breeding. The application is configured by hybridization of a wide-stigma indica rice maintainer line Chuan 345B and a narrow-stigma high-quality indica rice maintainer line Chuan 106B into a BC4F5 positioning population, then a method of map-based cloning is used, SSR molecular markers are used for preliminary positioning, and finally qSTB-8 is finely positioned in a 11.2 kb segment between markers SG930 and SG950. Gene function analysis of qSTB-8 shows that a transgenic line Chuan 106B has a wide-stigma trait.

This invention provides a method based on upland cotton GhMP This research relates to the field of genetic engineering technology and describes a method for the coordinated regulation of cotton fiber elongation and Verticillium wilt resistance using genes, as well as transgenic cotton plants. The gene encodes a membrane protein, and experiments using overexpression and RNA interference transgenic lines have demonstrated its effectiveness. GhMP The expression level of this compound is positively correlated with fiber development and disease resistance: increased expression significantly increases fiber length and improves resistance to Verticillium wilt, while decreased expression leads to fiber shortening and weakened resistance. Further studies have shown that... GhMP Proteins are located on the cell membrane and can interact with... GhPIP2.7 and GhTIP1.1 Aquaporins interact and jointly participate in the transmembrane transport of hydrogen peroxide, forming a molecular complex that regulates fiber elongation and disease resistance. This invention provides a key target gene that combines quality improvement and disease resistance enhancement, offering a new strategy and gene resource for multi-trait molecular breeding of cotton, and has significant application value and promising prospects.

This invention discloses a TaSMT-5B gene for enhancing wheat resistance to Fusarium head blight and its applications. The TaSMT-5B gene belongs to the selenocysteine ​​methyltransferase family, catalyzing the methylation of SeCys to MeSeCys. It is stably and highly expressed in the high-MeSeCys-rich and Fusarium head blight-resistant variety Zheng 9023, and a "GTCGCCG" insertion site exists in the promoter region of this gene in the Zheng 9023 variety. The TaSMT-5B gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in five stable transgenic lines. After basal application of inorganic selenium, the severity of Fusarium head blight in the transgenic plants was alleviated. This invention has significant production implications for achieving selenium biofortification in wheat and enhancing its resistance to Fusarium head blight, providing a new gene resource for breeding stable-yielding and high-quality wheat varieties.

This invention discloses lncRNA ABAC1 This invention relates to the application of improving the drought resistance of oats and falls under the field of plant genetic engineering technology. It is the first time that long non-coding RNA has been identified from oats. ABAC1 The nucleotide sequence of this lncRNA, as shown in SEQ ID NO.5, confirms that it is a negative regulator of drought resistance in oats, and its expression is significantly inhibited by drought stress. This was achieved by constructing the lncRNA... ABAC1 Oat transgenic lines with significantly downregulated expression of the gene were obtained by transforming oats with an RNAi silencing vector. Drought phenotype, water loss rate, and survival rate assays showed that silencing lncRNA... ABAC1 This invention can significantly reduce the rate of water loss in oat plants, improve survival rate under drought stress, and alleviate wilting damage, thereby significantly enhancing drought resistance. It provides novel gene targets and regulatory strategies for molecular breeding of drought-resistant oat varieties, and can be used for the breeding of new drought-resistant oat varieties, possessing significant theoretical value and application prospects.

The application provides application of BnaA09.ARGOS gene in regulation of seed development, and belongs to the technical field of biotechnology; a full-length BnaA09.ARGOS coding sequence is cloned, a 35S promoter driven overexpression vector and a CRISPR vector are constructed, and two kinds of vectors are used to transform Brassica napus to obtain stable transgenic plants respectively; through research, it is found that the seed quantity of the overexpression transgenic line is increased, the seed volume is increased, and the seed weight is increased, while the seed quantity of the CRISPR transgenic line is reduced, the seed volume is reduced, and the seed weight is reduced, the application of BnaA09.ARGOS gene in influencing seed volume, seed quantity and seed weight is verified, the BnaA09.ARGOS gene can be used for increasing the yield of Brassica napus, and has important guiding and reference significance for production and breeding of economic crops such as Brassica napus.

This invention discloses a gene, TaLAC58, capable of enhancing wheat resistance to Fusarium head blight and its applications. The TaLAC58 gene belongs to the laccase family and is a gene specifically expressed in the resistant wheat variety Sumai 3. This gene was overexpressed using a constructed overexpression vector and introduced into the popular variety Fielder via Agrobacterium infection, resulting in stable transgenic plants. In the three transgenic lines obtained, resistance to Fusarium head blight was significantly enhanced. This invention has significant production implications for improving wheat resistance to Fusarium head blight and provides a new gene resource for breeding high-yielding, stable-yielding, and widely adaptable new wheat varieties.

This invention relates to the fields of plant genetic engineering and potato breeding technology, and particularly to the application of a heat-inducible promoter in potato breeding regulation. This invention provides transgenic lines expressing SP6A and SP6AM induced by pHSP101 and pHSP70. Compared with the wild type, these transgenic lines exhibit improved tuber formation ability at both normal and high temperatures. This invention evaluated the tuber formation phenotype of the induced transgenic lines using two methods (in vitro high-sugar tuber induction system and conventional soil-cultured potatoes). The results showed that the transgenic lines had tuber formation ability under high temperatures compared to the wild type. This invention used qRT-PCR and laser confocal microscopy to detect the transcriptional and protein expression levels of the SP6A gene in the heat-induced lines, demonstrating that the promoters pHSP70 and pHSP101 respond to high temperatures to promote potato tuber formation, providing genetic materials and a theoretical basis for elucidating the mechanism of heat-resistant tuber formation in potatoes and for heat-resistant improvement breeding.

This invention discloses an integrated CRE / LOX system, comprising a binary vector backbone plasmid, a Cre-encoding gene for the cyclization recombinase, a LOX site specifically recognizable by Cre, and a reporter gene. As a biomarker gene expression platform, this integrated CRE / LOX system can generate transgenic lines for lineage tracing in specific cells in a single step by cloning the target gene promoter, without the need for secondary transformation or hybridization. This significantly reduces the experimental cycle and workload of cell lineage tracing in plants, providing an important tool for studying the development and regeneration of plant meristems and stem cells.