174results about How to "Reduce plant height" patented technology

The invention discloses a seed coating agent specially used for direct seeding rice, which is characterized in that the seed coating agent mainly contains the following ingredients by weight: 2 to 8 percent of super absorbent resin, 1 to 5 percent of oxygenates, 0.1 to 0.5 percent of sparrow and rat destruction agent , 0.5 to 2 percent of bactericide, 0.5 to 2 percent of pesticide, 0.02 to 0.2 percent of plant growth regulator, 3 to 10 percent of trace element fertilizer and the margin is special type mineral clay. The powder raw materials are crushed to more then 100 meshes and are mixed evenly, the mixture is coated on the surfaces of rice seeds by a seed-coating method for direct sowing, thus clay sacculus integrating 'a small water reservoir, a small oxygen reservoir, a small drug storage and a small fertilizer storage' are formed around the rice seeds and at the roots of seedlings; the seed coating agent has the effects of preserving and providing water, ventilating and providing oxygen, promoting seedling emergency, preventing diseases and killing pests, preventing mice and destructing sparrows, strengthening seedlings and roots and increasing production. The seed coating agent is also characterized by low dosage, low cost, simple and convenient operation, and the like, thereby being applicable to coating rice seeds which are directly sowed both on flooded paddy fields and dry paddy fields.

The invention provides a rice plant height related gene OsHPH, and an application to regulating the height of a rice plant. The gene is edited through a CRISPR-cas system to reduce the height of the rice plant at different degrees, and a T0-generation is subjected to segregation of posterity to breed a plant having no transgenic ingredients. The plant can be produced and applied as a crossing parent, and the undesirable phenomenon that a crossbred F1 is too high to cause lodging in the agricultural production is solved.

The invention belongs to the field of rapeseed genetic engineering and relates to a gene for controlling a rape plant type, a molecular marker and an application thereof. The gene for controlling a rape plant type can control plant height, branching angles and leaf morphology. Through map-based cloning and genetic transformation, a gene BnaA3. IAA7 for controlling a rape plant type is cloned and verified. The gene is located on an A3 chromosome of a rape genome, codes an auxin protein and has a sequence shown in the formula of SEQ ID NO: 1. The invention also discloses a separated mutant geneBnaA3. Iaa7 of the gene BnaA3. IAA7. The mutant gene has a sequence shown in the formula of SEQ ID NO: 4 and can significantly reduce the plant height and branching angles of rape. The mutant gene does not influence the yield per plant when improving the rape variety or the inbred plant type, and increases the yield per plant when improving the rape hybrid plant type. The invention also disclosesa related molecular marker.

The invention provides a wheat crop ridge seeding machine, relating to the technical field of agricultural machinery, in particular to a wheat crop seeding machine. A frame of the wheat crop ridge seeding machine is connected with a traction locomotive by virtue of a suspension traction frame at the front end, a driving wheel is arranged at the rear end of the frame, a seed storage tank is arranged at the upper part of the frame, a seed sowing device is arranged on the seed storage tank, and the driving wheel is connected with the seed sowing device and drives the seed sowing device by virtue of a transmission chain; the lower part of the frame is sequentially provided with a depth limiting wheel, a single-winged ridging plough, a ridging compacting machine, a seed opener, a ditch cleaning shovel and a ridge reshaping device; and the seed opener is connected with the seed sowing device by virtue of a seed drop tube. A suspension traction system, a ridge body moulding system and a precision drilling system of the seeding machine ensure earthing of a seeding trench to be uniform and ridge trench and ridge surface to be uniform, thus multiple processes such as ridging, sowing and the like can be completed at the same time, and agricultural requirements of high ridging quality, uniform seed discharging and consistent sowing depth can be met.

The invention discloses a method and application of knocking out a BnMAX1 gene in Brassica napus by a CRISPR-Cas9 system, two sgRNAs of specifically targeting Brassica napus BnMAX1 gene are designed and synthesized into oligo dimer, are connected with Cas9 vector, and introduced into hypocotyl callus of Brassica napus by Agrobacterium tumefaciens-mediated transformation to regerated into shoots. Cas9 nuclease was guided by sgRNA to cleave target sequence. Each sgRNA could pass through CRISPR-Cas9 system mediates the cleavage of BnMAX1 gene in A and C genomes, and achieves the goal of gene knockout. Phenotypic identification showed that homozygous mutant lines increased the number of branches and pods per plant, decreased plant height and increased yield.

The invention provides a method for establishing and planting a zoysia and trifolium repens mixed lawn. According to the technical scheme, the technologies that germination is accelerated in warm water, seeds are soaked in liquid medicine, the mixed sowing ratio is scientific, the dry and wet seed sowing quantity conversion method and the layered sowing and layered covering method are adopted, the fertilization amount and the watering amount are controlled, and the temperature, the humidity and light are controlled through agricultural plastic mulching film punching are applied, synchronous germination of the grass seeds of the mixed lawn is guaranteed, and various conditions required from seedling growth to lawn generation are satisfied. The method comprises the steps of seed processing, mulching film punching, field preparation conducted before lawn establishment, sowing, control over the temperature and the humidity in the germination stage, maintenance of seedlings inside a mulching film and the like. The novel method for establishing and planting the zoysia and trifolium repens mixed lawn is capable of solving the problems that a cold-season lawn is high in maintenance cost and prone to degeneration and a warm-season lawn is short in green period, influences the green effect and is difficult to popularize.

A durable-acting fertilizer with growth regulating function for paddy rice is prepared through proportionally mixing the raw fertilizer with the substance for making nutrients durable for 3-5 min, spraying growth regulating substance for coating, and stirring for 3-5 min. Its advantage is high yield.

The invention relates to a plant growth regulator characterized by that, the regulator is a liquid mainly comprising ethrel and butylacetic diethylaminoethanol easter and is prepared by mixing the two ingredients with added solution, stabilizer and surface active agent. The regulator by the invention can effectively prevent corn lodging, its advantages includes better yield increasing effect, appreciable attribute betterment, simple process for manufacturing, and easiness in use.

The invention belongs to the technical field of corn planting and particularly relates to corn planting and land improvement synchronous equal ratio empty cave bunch planting and supporting cultivation. The planting method comprises the following steps of land preparing and ridging, equal ratio empty cave bunch planting and fertilization, harvesting, stalk application and one-year fallow land improving, transferring rotted stalk into an organic fertilizer, and crop rotation planting. The equal ratio empty cave bunch planting method is adopted, corns can be well ventilated with light transmitting in both transverse and vertical directions, therefore, during the growth of corns, light energy absorption and light energy utilization and transformation in high photosynthetic efficiency can be achieved, increases of the corncob row number, the row niblet number and the thousand kernel weight are promoted, the formation of large corncobs is promoted, the lodging resistance of the corns is enhanced, the water content of the corns drops by 2-5%, and the yield increase range of the corns can reach 5-20%. According to the equal ratio empty cave bunch cultivation, 50% of a land can be fallowedand improved, fertilization and land fertility are achieved, the soil is loosened, surface water is preserved, and 40-50% of fertilizer usage amount is reduced. The corns can be planted organically and efficiently in a green and low-carbon mode.

The invention discloses a high-efficiency cultivation method of Job's tears, which comprises the following planting steps, including site selection and preparation, seed treatment, timely sowing, field management, and timely harvesting. Due to the combination of the above technical solutions, compared with the prior art, the present invention optimizes the selection and preparation of the coix coix, pre-sowing seed treatment, sowing methods, post-emergence field management and harvesting, and combines it with mechanization, reducing At the same time as the labor force, it also improves the yield and quality of coix coix, reduces input, and the method is easy to master, easy to implement, and has the characteristics of good implementation effect. Higher economic and social benefits.

The invention relates to the technical field of plant gene engineering, in particular to a method for increasing the grain number per ear and reducing plant height by use of rice SNB genes. The nucleotide sequence of SNB genes is shown as SEQ ID NO:1. Artificially mutant rSNB genes are obtained by changing the binding site of microRNA172 in the SNB genes, the nucleotide sequence of the rSNB genes is shown as SEQ ID NO:2, and the sequence of protein encoded by the rSNB genes is shown as SEQ ID NO:3. The grain number per ear of rice is remarkably increased and the plant height of the rice is notably reduced through overexpression of rSNB, and further studies prove that SNB encoded protein has the activity of a transcription suppressor. The functions of the SNB genes on increasing the grain number per ear of the rice and reducing the plant height of the rice are disclosed, and a novel rice genetic improvement method is also provided.

The invention discloses a Malus sieversii (Ledeb.) Roem-derived plant growth-related protein, and a coding gene and application thereof. The protein provided by the invention is: a) a protein of which the amino acid sequence is shown as SEQ ID NO.2; or b) a protein which is obtained from SEQ ID NO. 2 through substitution and / or deletion and / or addition of one or more amino acid residues, is related to plant growth and is derived from a). The protein and the coding gene thereof can be used for regulating and controlling plant growth.

The invention relates to an organic liquid fertilizer for promoting a rhizome increase and methods for preparing and applying the organic liquid fertilizer. The organic liquid fertilizer is characterized by comprising the following raw materials by weight: 0.005-0.15% of A-type fungus, 0.5-5% of brown sugar molasses, 0.15-1.5% of bact-peptone, 0.005-0.15% of B-type fungus, 0.25-0.5% of fish milt, 0.15-1.5% of animal giblets containing a liver and / or a gallbladder, 0.05-1% of seaweeds, and the balance of water; the A-type fungus comprises any one or more of azotobacter, nitrobacteria, phosphorus-solubilizing bacteria, saccharomycetes, lactic acid bacteria, photosynthetic bacteria, actinomyces, and growth bacteria; the organic liquid fertilizer has the benefits that the yield can be increased by using the liquid fertilizer with low concentration. Through spraying and fertilizing of the organic liquid fertilizer, the height of plants can be lowered, and the yield can be increased; moreover, the fertilizing method of the organic liquid fertilizer can directly collocated with either spraying pesticide or sprinkling irrigation and the convenience is brought for application. The organic liquid fertilizer contains a great amount of the beneficial microbes and the metabolites of the microbes after sprayed, so that the disease can be prevented and the yield can be increased by 10-25% or higher.

The invention is suitable for the technical field of molecular biology, and provides a mutant gene and mutant of corn gibberellin oxidase, an expression vector and applications. The nucleotide sequence of the original gene of the corn gibberellin oxidase is shown as SEQ ID NO: 1 in a sequence table, and the nucleotide sequence of the mutant gene is shown as SEQ ID NO: 2 or SEQ ID NO: 3 in the sequence table. The mutant gene of the corn gibberellin oxidase provided by the embodiments can be used for building the plant expression vector to convert into crops such as arabidopsis thaliana; the life states such as phenotype and survival rates of transgenic arabidopsis thaliana can be observed before and after drought stress treatment; and through the indication of results, the mutant gene of the corn gibberellin oxidase can lower the plant height of plants such as the arabidopsis thaliana and can also enhance the drought tolerance of the arabidopsis thaliana and other plants.

The invention discloses application of a phospholipase gene PLDzeta1 in improving salt resistance of a plant. The gene PLDzeta1 is transferred to a plant through a molecular genetic transformation method, and the transformation plant with the overexpression gene PLDzeta1 is obtained. The overexpression gene PLDzeta1 remarkably improves the salt resistance of the plant. The membrane permeability and the malondialdehyde content of a leaf of the plant with overexpression gene PLDzeta1 are remarkably lower than those of a control wide group under the threat of salt, and the survival rate and the plant height are remarkably higher than those of the control wide group. The loss expression of the gene PLDzeta1 leads to remarkable reduction of the salt resistance of the plant and remarkable reduction of biological yield compared with the wild group, and the result further improves that the gene PLDzeta1 has a remarkable positive regulation effect on salt resistance. The application of the phospholipase gene PLDzeta1 in improving salt resistance of the plant provides a novel way for the seed breeding of salt-resistance molecules of the plant, and creates a novel seed material.

The invention relates to a plant growth regulator characterized by that, the regulator uses mepiquat chloride and butylacetic diethylaminoethanol ester as the predominating constituents with added carrying agent, stabilizing agent and surface-active agent after mixing. The advantages of the invention are, effective prevention of soybean lodging, better yield increasing effect, appreciable attribute betterment, simple process for manufacturing, and easiness in application.

The invention discloses application of paclobutrazol to controlling the plant type of Chinese cymbidium or preparing preparations for controlling the plant type of Chinese cymbidium, and the optimal method for controlling the plant type of Chinese cymbidium through paclobutrazol is explored and optimized, that is, paclobutrazol is sprayed to leaves of Chinese cymbidium in a leaf spraying mode so that the surfaces of the processed leaves can be wet. Preferably, when plants grow to 5-15 cm, 1000-1500 mg / L paclobutrazol is used for processing, the plants are processed every 10-20 days, and processing is conducted for three to four times. The method greatly improves the function of paclobutrazol on Chinese cymbidium, can remarkably reduce the plant height of Chinese cymbidium, and guarantees that the width of plant leaves is not affected, the plant type is more compact, the processed plant type of Chinese cymbidium is more attractive, and the method can be applied to cultivation and production on a large scale.

A gene OsHT1 controlling rice tillering is disclosed. The gene is derived from oryza sativa L. cv. Zhonghua 11. The nucleotide sequence of the gene is SEQ ID NO.1, the nucleotide sequence of the open reading frame of the gene is SEQ ID NO.2, and an amino acid sequence encoded by cDNA of the gene is SEQ ID NO.3. Phenotypic analysis shows that after overexpression of the gene OsHT1, the tiller number of rice is increased from about 20-27 to 135-145, plant height is reduced and the setting rate is not changed. The gene OsHT1 is a critical regulating gene for rice tillering, and therefore rice tillering characters can be regulated by utilizing a genetic engineering technology, and the gene has important application value in the field of regulating and controlling plant shape characters on purpose by utilizing a bioengineering technology, and adjusting a plant population structure to achieve a high yield and good quality. The gene can be used for cultivating high-yield varieties of rice, wheat, and other cereal crops.

The invention discloses a compound high-efficiency wheat seed coating agent. By grinding and mixing, the compound high-efficiency wheat seed coating agent is prepared from a pesticide, a bactericide, a plant-growth regulator and trace elements as effective components, and a wetting agent, a dispersant, an emulsifier, a thickening agent, a film forming agent, an antifreezing agent, an antifoaming agent, a mildew inhibitor, a pH conditioning agent and a warning colouration agent as assistants. The compound high-efficiency wheat seed coating agent can effectively prevent and treat diseases and insect pests and nutrient deficiency symptom of wheat in a seedling stage and can strengthen seedlings and promote rooting thereby improving wheat lodging resistance.

The invention discloses a method for cultivating a transgenic gramineous plant with increased tiller number and a biological material related with the method. The method comprises the following steps: guiding a DNA (Deoxyribonucleic Acid) molecule PGH3-IPT into a receptor-gramineous plant to obtain the transgenic gramineous plant with increased tiller number, increased spike number and / or reduced plant height in comparison with the receptor-gramineous plant. Compared with the receptor-gramineous plant, the transgenic gramineous plant obtained by guiding the DNA molecule PGH3-IPT into the receptor-gramineous plant has the characteristics that malformation caused by excess change of plant morphology can not be caused when the effective tiller number and plant height of the transgenic gramineous plant are properly reduced; the spike number and grain weight of the transgenic gramineous plant are not remarkably changed, so that the yield of a single gramineous plant is increased; other morphology and growth and development characteristics of the plant are not remarkably changed.

The invention belongs to the technical field of plant propagation and discloses a plant growth regulator composition for ryegrasses. The plant growth regulator composition is prepared from uniconazole and an auxin plant growth regulator with the mass ratio of (2-8) to (6-10). When being used for treating ryegrass seeds, the plant growth regulator composition can remarkably increase the rooting rate of the ryegrasses, and the interaction effect of the uniconazole and the auxin plant growth regulator on the growth of ryegrass seedlings is remarkable, the quality of the ryegrass seedlings is remarkably improved, the sprouting and the growth of the ryegrasses are facilitated, the lodging resistance and the severe environment resistance of the ryegrasses are improved and the plant growth regulator composition is beneficial to the promotion and the application of the ryegrasses.

The invention discloses a greenhouse dwarfing and potting method of an eustoma grandiflorum cut flower variety. Through gardening measures and ecological factor adjustment, the method includes the steps of conducting processing for 10-11 hours at low night temperature of 10-13 DEG C in short daylight, conducting micro pinching 1-2 times and spraying cytokinin in the early period of eustoma grandiflorum production, conducting processing at high night temperature of 18-22 DEG C for long illumination duration of 14-16 hours in the later period of production, and cooperatively taking measures like applying of dwarfing agent in the bolting and flowering period and semi-arid management. By means of the method, the greenhouse dwarfing and potting of the eustoma grandiflorum cut flower variety are achieved, and the defect that eustoma grandiflorum potted varieties mainly include single-petal small flowers and are few in variety number and simplex in flower color and flower shape are overcome. By means of the method, the eustoma grandiflorum potted product which is rich in flower color, diversified in type, long in ornamental period, large in flower and heavy in petal can be obtained.

The invention relates to a rice B-type reaction regulation and control factor gene ORR2 and application of the gene. An ORR2 gene cDNA reading frame is in a nucleotide sequence shown in SEQ ID NO:1 ora nucleotide sequence completely in complementary pairing with SEQ ID NO:1 or a coding amino acid sequence, such as a nucleotide sequence shown in SEQ ID NO:2. An ORR2 gene overexpression vector is established, and the height of a rice overexpression plant with the established ORR2 gene is obviously lowered. The rice B-type reaction regulation and control factor gene ORR2 can be applied to regulation and control over rice plant-type dwarfing and dwarfing cultivation.

The invention provides an ecological system controlling method, and particularly relates to a method of determining pasture-hybridized chinese pennisetum and / or jerusalem artichoke through screening to control cenchrus paciflorus. The pasture-hybridized chinese pennisetum and / or the jerusalem artichoke are / is used for controlling the cenchrus paciflorus, by means of reasonably collocating hybridized chinese pennisetum and / or the jerusalem artichoke, scientific seeding and management are matched, the growth advantages of the hybridized chinese pennisetum and / or the jerusalem artichoke are used for contending against the cenchrus paciflorus, the intrusion behavior of the cenchrus paciflorus is restrained continuously and effectively, and a guarantee is provided for controlling the alien invasive species cenchrus paciflorus.

The invention discloses a method for shortening the juvenile span by grafting hybrid seedling rootstocks, and the method comprises the following steps of: (1) scion preparation: collecting full buds of biennial hybrid seedlings as scions, wherein the buds are located at places 20-30cm downward from the tops of the trunks of the biennial hybrid seedlings; (2) rootstock preparation: horizontally shearing the lower ends of the roots of the trunks of the biennial hybrid seedlings to serve as rootstocks, wherein the shearing places are 10-15cm upward from the ground; (3) grafting: directly grafting the collected scions on the rootstocks by using a cleft graft; and (4) wrapping: tightly wrapping all the sheared edges by using plastic films. According to the method, the heights (namely, the juvenile span) of the fruiting parts of the hybrid seedlings can be decreased by more than 1.2m and then the whole heights of the trees are decreased, so that the wind resistance of the seedlings is strengthened and the problems of tree lodging, branch broking, fruit falling and the like are prevented, convenience is brought to the field operations such as fruit thinning, bagging, pruning and the like, and the field management workers of the hybrid seedlings are decreased.

The invention discloses a method for planting dry-land Brassica napus by using mulching film corn residual films, which is characterized in that the residual films are reserved after mulching film corns are harvested, and the Brassica napus is dibbled by using broken films according to the original specifications, i.e. the ridge width being 1.0m to 1.2m and the ridge spacing being 0.3m to 0.5m, the row spacing being 20cm to 23cm, the plant spacing being 25cm to 30cm, 0.2kg to 0.3kg of seeds per mu land and 5 to 8 grains per pit; when 3 to 5 main leaves grow from the Brassica napus, final singling of seedlings is completed at one step, two seedlings per pit are left and the density is controlled to be 18000 to 20000 plants per mu; and when 6 to 8 main leaves grow from the Brassica napus, the residual films are uncovered, intertillage soil loosing is conducted in combination with application of 8kg to 10kg of urea to promote the seedlings and the soil among ridges is lifted up to the ridges for planting the Brassica napus. By reusing the residual films, the method effectively solves the problems of insufficient soil moisture, low ground temperature, uneven emerged seedlings, poor seedling growth and high field management labor input from the sowing to the stage of the dry-land Brassica napus with 8 main leaves, realizes the tillage-free and drought-resistant cultivation of the Brassica napus, and achieves the goals of cultivating strong seedlings, saving the cost and increasing the efficiency during Brassica napus production.

The invention discloses application of a ZmSBP12 gene in regulation and control of drought resistance, plant height and ear height of corn. After the ZmSBP12 gene is overexpressed in corn, a corn mutant plant has the characteristics of increased drought resistance, reduced plant height, reduced ear height and the like. Overexpression of ZmSBP12 leads to increase of drought resistance and reductionof plant height and ear height, indicating that the ZmSBP12 gene plays a crucial role in drought resistance, plant type (plant height) and the like of corn. By improving the expression abundance of the ZmSBP12 gene, the effects of improving the drought resistance of the corn, reducing the plant height and ear height of the corn and the like are achieved, and the ZmSBP12 gene can be applied to auxiliary breeding of new drought-resistant and lodging-resistant corn varieties and can also be applied to cultivation of excellent selfing lines and hybrids of the corn.

The invention discloses a wheat temperature-light-sensitive sterile line trans-breeding method. Aiming at unique natural weather of Huainan wheat areas of Jiangsu Province, a wheat sterile line material high in resistance to red peel, dwarf and scab is trans-bred; according to genetics laws, excellent characters are transferred into two different line materials through sexual mating and gene recombination. A novel sterile line material is obtained through improvement, resistance to ear sprouting and scab, resistance to ear sprouting and scab, plant height is reduced, and the method has favorable effect on lodging resistance of matched offspring variety. Through tissue cultivation technology, homozygosis time is reduced greatly, and breeding life is shortened.

The invention discloses plant strain associated proteins PROG 2 as well as an encoding gene and application thereof. The plant strain associated proteins PROG 2 provided by the invention are as follow a1) or a2) or a3), wherein a1) is a protein with an amino acid sequence shown in a sequence 2 in a sequence table; a2) is a fusion protein obtained by connecting a label to a N terminal and / or a C terminal of the protein shown in the sequence 2 in the sequence table; a3) is a plant strain associated protein obtained by substitution and / or deletion and / or addition of one or more amino acid residues of the protein shown by a1) or a2). Experiments prove that the plant strain associated protein PROG 2 has a very important role in regulating and controlling the rice plant type and the yield, and has a wide prospect in cultivation of new varieties of high-yield rice.