15results about How to "Reduce plant height" patented technology

The invention discloses an application of a rice OsRNU7 gene. The registration number of the nucleotide sequence of the OsRNU7 gene in Genbank is LOC4337677, and the registration number of the nucleotide sequence of the OsRNU7 gene is LOC4337677. RNA-seq is utilized to discover that the OsRNU7 shows response to the external nitrogen supply level, and fluorescent quantitative PCR (Polymerase Chain Reaction) proves that the OsRNU7 is induced by high nitrogen. The OsRNU7 is knocked out, so that the nitrogen absorption rate, the plant height and the yield of the rice are reduced, and the comprehensive performance is that the nitrogen fertilizer utilization efficiency is reduced. The function of the OsRNU7 gene for positively regulating the utilization efficiency of the rice nitrogen fertilizer is defined, and a technical route for improving the utilization efficiency of the rice nitrogen fertilizer by increasing the expression of the OsRNU7 in rice is provided.

This invention provides a maize plant architecture regulating gene ZmEXO1 and its application, belonging to the field of maize molecular breeding technology. The amino acid sequence of ZmEXO1 is shown in SEQ ID NO.7. Studies have found that ZmEXO1 can regulate maize leaf angle and plant height; mutants with reduced or silenced ZmEXO1 expression lead to smaller leaf angles and reduced plant height. Therefore, gene editing of the ZmEXO1 gene or genetic breeding improvement using Zmexo1 low-expression mutants can obtain maize germplasm with improved plant architecture, which is of great significance for enriching maize germplasm and breeding high-yielding, dense-planting-tolerant varieties. This invention also provides a Kasp molecular marker and Kasp primers closely linked to ZmEXO1, which can be used for marker-assisted selection breeding and the discovery of related functional genes, enabling rapid screening and identification of maize germplasm containing ZmEXO1-modified molecules.

The invention discloses a wild barley HbAUX1 gene and application, belongs to the technical field of plant genetic engineering, the nucleotide sequence of the HbAUX1 gene is shown as a sequence 1, the invention discloses application of the HbAUX1 gene in plant dwarfing and / or plant seed yield increasing, and contributes to cultivation of excellent high-yield dwarfed plants.

The invention discloses medicago truncatula plant type development control protein as well as a gene coded by the medicago truncatula plant type development control protein and application. The method comprises the following steps: firstly, finding a Tnt1 insertion mutant mini-1 with a changed plant type from a medicago truncatula Tnt1 insertion mutant library, cloning a control gene MINI for plant type development by combining whole genome re-sequencing with flanking sequence analysis, and then inversely screening a natural mutant mini-2 of the MINI gene in the mutant library in order to prove that the MINI is the control gene for plant type development. Meanwhile, a MINI gene knockout strain mini-3 is also constructed, and analysis finds that phenotypes of mini-2 and mini-3 are completely the same as those of a mini-1 mutant. The results prove that the MINI gene provided by the invention can regulate and control the plant type. The method lays a foundation for plant type development research of leguminous plants such as alfalfa and other dicotyledonous plants.

The application discloses a wheat plant type gene and application, and belongs to the technical field of genetic engineering breeding. The technical problem to be solved by the application is how to regulate the plant height or ear type of wheat. In order to solve the above technical problem, the application provides the application of a protein or biological material related to the protein in regulating the plant height and / or ear type of a plant, wherein the protein is QIP3 protein, and the QIP3 protein can be three of the three proteins with the amino acid sequences of SEQ ID NO:1, SEQ ID NO:2 and SEQ ID NO:3, any two or any one. The application mines a candidate gene QIP3 gene affecting the plant height and / or ear length of wheat, and the plant height of a knockout strain of the QIP3 gene is significantly reduced and the ear length is significantly increased. Research on the function of the QIP3 gene and application of the QIP3 gene in wheat breeding have important significance for improving the growth traits and yield of wheat.

This invention discloses the application of the maize ZmMKK1 gene in regulating plant height and ear height, relating to the field of genetic engineering technology. The nucleotide sequence of the ZmMKK1 gene is shown in SEQ ID NO.1, and the amino acid sequence of the protein encoded by the ZmMKK1 gene is shown in SEQ ID NO.2. This invention is the first to discover that inhibiting the expression of the ZmMKK1 gene, which encodes the ZmMKK1 protein, can significantly reduce maize plant height and ear height. This provides a sound theoretical basis for the genetic improvement of plant height and the cultivation of lodging resistance and high yield in maize, and also provides a scientific basis for further fully exploring the production potential of maize and increasing maize yield, showing promising application prospects.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to application of a PcWVD2 gene in enhancing the dwarfing ability of pear plants. The CDS (Coding Sequence) of the PcWVD2 gene is as shown in SEQ ID NO. 1. The amino acid sequence coded by the PcWVD2 gene is as shown in SEQ ID NO. 2. The invention reveals that the PcWVD2 gene has the function of regulating and controlling the dwarf character of the pear plant, the dwarf ability of the pear can be obviously enhanced by over-expressing the gene, the dwarf ability shows that the plant height is reduced, the internode is shortened and the root length is shortened, and an important candidate gene is provided for dwarf close planting cultivation of the pear tree. According to the method for enhancing the dwarfing ability of the pear plant, the endogenous dwarfing gene PcWVD2 is directly overexpressed by the pear plant through genetic transformation, the stably inherited dwarfing character can be realized, the dependence on dwarfing rootstock can be gotten rid of, and the cultivation management cost can be reduced.

The application belongs to the technical field of molecular biology and crop breeding, and particularly relates to a STS marker completely linked with wheat heading stage and plant height, a primer, a distinguishing method and application thereof in breeding. The STS marker completely linked with wheat heading stage and plant height is G base insertion at the 68416769 base of wheat 7D chromosome. The application further provides a primer capable of amplifying the STS marker, and the primer comprises sequences as shown in SEQ ID NO:1 and SEQ ID NO:2 respectively. The STS marker is a functional molecular marker of wheat heading stage and plant height mutation gene, and can be used for molecular marker assisted selection and screening of wheat breeding materials. The site identified by the functional STS marker in the wild type and mutant can be used for improving the heading stage and plant height of wheat through backcross selection.

The invention discloses a method for creating specific germplasm of ultra-short-stem multi-branch sesame, and belongs to the technical field of plant breeding. The method comprises the following steps: pretreating Zhongzhi 14 seeds, mutagenizing the seeds by using an ethyl methanesulfonate solution as a mutagenic agent, performing conventional sowing to obtain a group M1, performing selfing and seed reserving to obtain a group M2, selecting excellent single plants from the group M2, performing selfing to obtain a group M3, performing continuous selfing to obtain a group M6, and obtaining a strain EM102 with stable characters from the group M6, obtaining the specific germplasm of the ultra-short-stem multi-branch sesame. The ultra-short-stem multi-branch sesame specific germplasm EM102 cultivated by the method has many and short branches, so that the lodging risk is obviously reduced; flowers are clustered, lip-shaped and pink white; the method has the advantages that the leaves are many and narrow, the stems are tender, cluster buds easily appear after the stems are broken, and the method can be used as a parent material for breeding the white oil and vegetable dual-purpose sesame and can also be used as a horticultural plant for cultivation and planting.

The invention belongs to the technical field of gene engineering, and particularly discloses a rice plant height regulation gene OsPH4 and application thereof, the nucleotide sequence of the gene OsPH4 is shown as SEQ ID NO.1, and the amino acid sequence of protein encoded by the gene OsPH4 is shown as SEQ ID NO.2; a gene OsPH4 of Liao 93B is mutated into an OsPH4 mutant gene through a chemical mutagenic agent, and then multiple generations of selfing are performed in a field to obtain the rice dwarf homozygous mutant osph4. Compared with the prior art, the gene OsPH4 for regulating and controlling the plant height is cloned in a rice dwarf mutant by utilizing a map-based cloning technology, and a functional complementation experiment proves that the OsPH4 is the gene for controlling the rice plant height. The OsPH4 gene affects the change of the plant height of the rice, and the whole growth period shows a dwarf phenotype; after the OsPH4 gene is mutated, the plant height of the OsPH4 gene in the whole rice growth period is obviously reduced, so that the OsPH4 gene can be used for cultivating dwarf rice varieties.

The invention provides an HIS mutant protein and application thereof in plant breeding, and particularly provides an HIS mutant protein, after the HIS mutant protein is overexpressed in plants, the plants can be endowed with resistance to herbicides, the plant height of the plants can be reduced, the yield can be increased, and the HIS mutant protein has very wide application prospects in biological breeding.

The application discloses application of a wheat TaSmD3 gene in improving lodging resistance of wheat and belongs to the technical field of plant genetic engineering.The application clones a wheat TaSmD3 gene from wheat, and finds through research on the gene that a mutant obtained by knocking out the TaSmD3 gene through CRISPR / Cas9 technology can reduce the plant height of the wheat and improve the lodging resistance of the wheat, and the ear length, the number of spikelets per ear, the number of fertile florets per ear, the number of grains per ear and the hundred-grain weight and other yield characters of the mutant have no significant difference from those of a wild type.Therefore, the wheat TaSmD3 gene can be used as a new dwarf gene, and under the premise that the yield characters of the wheat are not affected, the lodging resistance of the wheat is improved, which has important significance for improving the harvest index of the wheat and promoting high yield and stable yield.

The invention discloses an OsEME1 protein capable of changing leaf color and plant height of rice as well as a coding gene and application of the OsEME1 protein. The protein is A1) or A2) or A3) or A4) as follows: A1) a protein with an amino acid sequence as shown in SEQ ID NO: 2; a2) a protein which is obtained by substituting and / or deleting and / or adding one or more amino acid residues of the amino acid sequence as shown in SEQ ID NO: 2 and has the same function; a3) a protein which has 99% or more, 95% or more, 90% or more, 85% or more, 80% or more or 75% or more of identity with the amino acid sequence limited by any one of A1)-A2) and has the same function; and A4) a fusion protein obtained by connecting a label to the N end and / or C end of any protein defined in A1)-A3). Tests prove that the OsEME1 gene deleted rice plant shows obvious dwarfing and leaf color difference.

The invention relates to the technical field of ecological protection, in particular to a growth regulation and control method for potamogeton crispus. The growth regulation and control method comprises the combination of the following two means: 1) water surface shading; (2) planting submerged plants; the submerged plants comprise one or more of elodea nuttallii, waterweed, vallisneria natans and hydrilla varticillata. According to the growth regulation and control method for the potamogeton crispus, after implementation, the plant height of the potamogeton crispus can be reduced by 30% or above, the growth rate is reduced by 55% or above, and meanwhile removal of nitrogen-containing pollutants in a water body is facilitated. According to the submerged plant planting device, submerged plant planting is input at a time, the shading floating ball can be repeatedly used and recycled, operation is easy, cost is low, no chemical agent needs to be used, and the environment is clean and free of pollution. Compared with manual potamogeton crispus salvage, the workload of the potamogeton crispus salvage device lies in throwing and recycling the floating balls in spring every year, manpower and equipment expenses are greatly reduced, and water plant residues do not need to be cleared, transported and treated.

The application discloses application of a zinc-dependent DNA binding protein ZmPLATZ9 in regulating corn plant height, and belongs to the field of plant genetic engineering and corn molecular breeding.The application first finds that the ZmPLATZ9 gene has a biological function of regulating corn plant height and lodging resistance capacity.In corn, a ZmPLATZ9 gene CRISPR / Cas9 knockout vector is constructed, and a homozygous strain of a ZmPLATZ9 knockout transgenic plant obtained by the vector has a plant height and ear position height obviously lower than those of a wild type, that is, the plant height and ear position height of corn can be reduced by knocking out the ZmPLATZ9 gene, and the lodging resistance capacity of corn is improved.The application provides direct evidence for the ZmPLATZ9 gene regulating corn plant height and ear position height, and can be used for cultivating a semi-dwarf corn new variety and providing a train of thought for creating a high-yield and lodging-resistant corn new variety.