103 results about "Citrus plant" patented technology

The invention discloses a method for improving citrus yellow vein disease resistance by using a ClPsaN gene. The method comprises the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the citrus by the VIGS expression vector to obtain a citrus ClPsaN gene silenced VIGS plant. According to the invention, the VIGS vector of the Citrus ClPsaN gene is constructed, and then the Citrus is transformed, so that the obtained Citrus plant can show obvious resistance to the Citrus yellow vein clearing virus, the titer of the Citrus yellow vein clearing virus can be reduced by 22-34%, the accumulation amount of the virus protein is reduced by 35-50%, and the attack degree of the Citrus yellow vein clearing virus is obviously reduced; the TRV silencing of the citrus ClPsaN gene does not influence the phenotype of a citrus plant, and the citrus ClPsaN gene can be used as a candidate gene for citrus yellow vein disease resistance breeding together with a plurality of citrus yellow vein disease-resistant and susceptible genes by utilizing the cooperation of VIGS silencing, RNA interference, gene editing and other technologies.

This invention discloses a wind-solar-storage multi-energy complementary water lifting and rainwater harvesting coordinated irrigation system, relating to the field of intelligent irrigation. The system includes: a power unit, a rainwater harvesting unit, a water lifting unit, sprinkler irrigation equipment, and a drone formation. The drone formation is used to fly carrying the sprinkler heads and water pipes of the sprinkler equipment under the control of the control system, and to adjust the sprinkler head's spray position under the control of the control system, and to maintain a safe distance between the water pipes in the sprinkler equipment and ground obstacles under the control of the control system. This invention can realize intelligent and low-cost irrigation for citrus planting in hillside areas.

The atomization device of the plant protection unmanned aerial vehicle for citrus planting comprises a top mounting base and a connecting arm, two through holes are symmetrically formed in the lower portion of the top mounting base, a first fixing hole is formed in the top of the connecting arm, and a connecting rod slidably penetrates through the first fixing hole and the two through holes jointly; through cooperative use of the suction mechanism and the heat dissipation mechanism, rapid heat dissipation of the servo motor is achieved, sucked air enters the bottom shell through a one-way valve, the interior of the bottom shell is pressurized, centrifugal atomization of the atomization mechanism is matched, pressurized atomization and centrifugal atomization are achieved, the atomization effect is good, and the service life of the servo motor is prolonged. The water flow is preliminarily segmented by the first segmentation fin, the second segmentation is performed by the cutting column, and the third segmentation is performed by the second segmentation fin, so that the multi-stage segmentation of the water flow is realized, the atomization effect is further improved, and the coaxial opposite rotation of the first-stage atomization assembly and the second-stage atomization assembly can be realized by the single servo motor.

The invention discloses a Citrus Huanglongbing resistant protein CsALD1-1 and a method for improving the resistance of citrus plants to Citrus Huanglongbing. The amino acid sequence of the CsALD1-1 protein is as shown in SEQ ID NO: 1, and the nucleotide sequence of the CsALD1-1 gene is as shown in SEQ ID NO: 1. According to the method for improving the resistance of citrus plants to citrus huanglongbing, ALD1 gene is utilized to encode lysine transaminase protein to promote the content of NHP in citrus, so that the whole-body immunity of citrus is activated, and the resistance to huanglongbing is enhanced. The CsALD1-1 overexpressed transgenic plant obtained by the invention has the advantages that the content of CLas is obviously lower than that of a wild type plant, and the attack degree of the citrus huanglongbing can be effectively and obviously reduced, so that the resistance of the citrus to the citrus huanglongbing is obviously improved, the phenotype of the transgenic plant is not influenced, and the CsALD1-1 overexpressed transgenic plant has a great application value for the citrus huanglongbing-resistant breeding.

The invention belongs to the technical field of plant genetic transformation, and particularly relates to a method for rapidly obtaining a citrus rootstock genetic transformation root system and application thereof. According to the genetic transformation method, agrobacterium rhizogenes K599 is used as a medium, an infection solution is transfected through a wound of a citrus plant, the infection solution can be quickly transfected into a stem segment of the citrus plant, and finally, the genetically transformed citrus plant can be quickly obtained in a high-pressure propagation mode. The method provided by the invention can effectively shorten the transformation system period, reduce the genetic transformation cost, improve the genetic transformation efficiency and avoid the generation of chimeras, and the materials can be repeatedly utilized.

The present invention relates to a product containing elderberry extract and a potentiator selected from pepper extract, ginger extract, curcuma extract and citrus plant extract.

The invention discloses an intelligent slow-release nano-pesticide, a preparation method thereof and application of the intelligent slow-release nano-pesticide in prevention and control of diaphorina citri. The preparation method comprises the following steps: adding a thiamethoxam methanol solution into a 2-methylimidazole solution, ultrasonically dispersing, adding a zinc nitrate solution while stirring, continuously stirring for 1 hour at room temperature, centrifuging, washing, and removing thiamethoxam to obtain THX ZIF-8 nanoparticles; the preparation method comprises the following steps: adding THX-coated ZIF-8 nano-particles into a Tris-HCl buffer solution, stirring and dispersing, adding dopamine hydrochloride, stirring, centrifuging and washing at room temperature, repeating for three times, and drying overnight to obtain THX-coated ZIF-8 / PDA solid powder, namely the intelligent slow-release nano-pesticide. The nano pesticide not only can effectively prevent and treat diaphorina citri, but also can provide a new strategy for comprehensive prevention and control of the citrus liberobacter asiaticum by reducing the ROS level in plants, enhancing the immunity of the plants and reducing proliferation of the liberobacter asiaticum in the citrus plants.

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention discloses a method for improving citrus liberobacter asiaticum resistance by using a CsZRK1 gene, the CsZRK1 gene is mutated to improve the liberobacter asiaticum resistance of citrus plants, and the nucleotide sequence of the CsZRK1 gene is as shown in SEQ ID NO: 1. The CsZRK1 gene coding sequence is cloned, the CRISPR / Cas9 mutant vector is constructed, and then the citrus is converted, so that the incidence degree of the hairy root huanglongbing of the obtained transgenic plant is obviously reduced, and the CsZRK1 gene has a great application value for huanglongbing-resistant breeding of the citrus, and can be used as a candidate gene for huanglongbing-resistant breeding with a plurality of huanglongbing-resistant and susceptible genes.

The invention discloses a method for improving citrus liberobacter asiaticum resistance by using CsFMO1-1 protein and a disease-resistant citrus, and the citrus liberobacter asiaticum resistance of citrus plants is improved by adjusting the expression level of CsFMO1-1 gene in the citrus plants; the method specifically comprises the following steps: (1) cloning a citrus CsFMO1-1 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic plant of which the expression level of the CsFMO1-1 gene is up-regulated. The citrus CsFMO1-1 gene coding sequence is cloned, an overexpression vector of the citrus CsFMO1-1 gene is constructed, then citrus is converted, the content of CLas in an obtained CsFMO1-1 overexpression transgenic plant is obviously lower than that of a wild type, the incidence degree of the citrus huanglongbing can be effectively and remarkably reduced, and therefore the resistance of the citrus to the citrus huanglongbing is remarkably improved; the phenotype of a citrus plant is not influenced, and the gene has an important application value for citrus liberobacter asiaticum resistance breeding, and can be used as a candidate gene for citrus liberobacter asiaticum resistance breeding with a plurality of citrus liberobacter asiaticum resistance and susceptible genes.

The utility model provides a fertilizer proportioning and stirring device for citrus planting, and relates to the technical field of citrus planting, the fertilizer proportioning and stirring device comprises a stirring device main body and a working mechanism, the working mechanism is arranged on one side of a sealing cover, and through the arrangement of the working mechanism, when a stirrer needs to be cleaned, a handle is shifted upwards, and the stirring device is started; when the stirrer is used, the handle moves upwards through the first rotating shaft and the second rotating shaft, meanwhile, the first hasp arm moves downwards through the second rotating shaft and extrudes the spring, the spring generates elastic force after being pressed, then the second hasp arm is driven to move upwards, the clamping roller is separated from the clamping base, and therefore the stirrer can be taken down from the connecting base to be cleaned. After cleaning is completed, the clamping roller is moved to the position above the clamping base according to the method, at the moment, the handle is loosened, the spring releases potential energy to push the first hasp arm back to the original position, the second hasp arm also resets, and therefore the clamping roller is tightly clamped into the clamping base, the stirrer is convenient to disassemble, and the service life of the stirrer is prolonged.

The utility model discloses a citrus is planted and is used fertilizer mixing device, including mixing shell, the opening of mixing shell top movable cover has top cover, the rotatory setting of mixing shell is in the central shaft, the movable insertion of several stirring rods has in the central shaft. The utility model discloses through the mutual cooperation of mixing shell, top cover, central shaft, stirring rod, auxiliary rod, feeding port, discharge cover, drive plate, hydraulic cylinder no.

The invention belongs to the technical field of agricultural planting, and discloses an intelligent citrus planting and picking management system and method.In the citrus growing season, a data acquisition module acquires data in real time and transmits the data to a data analysis processing module, and after analysis, an intelligent decision module generates a fertilization instruction and an irrigation instruction; the execution control module controls the fertilization equipment and the irrigation equipment to perform periodic fertilization and irrigation, and the fertilization and irrigation period is determined according to the soil type, the local meteorological data and the citrus tree growth stage; when the data analysis processing module finds that citrus leaves have disease and pest symptoms through image recognition, the intelligent decision-making module recommends the corresponding prevention and control pesticide amount, the execution control module controls the disease and pest prevention and control equipment to perform pesticide application operation, and the camera continuously shoots fruit images near the picking season. And the data analysis processing module judges the fruit maturity through image analysis, and when the maturity is qualified, the picking robot is controlled to pick.

A seedling transplanting fixed-distance auxiliary device for citrus planting relates to the technical field of citrus planting and comprises a shell which is of a rectangular block structure with a hollow interior; the planting assemblies are linearly arranged at intervals in the length direction of the shell, each planting assembly freely penetrates through the shell in the direction perpendicular to the length direction of the shell and is slidably connected with the shell, each planting assembly is provided with two planting positions, and the two planting positions are located on the two sides of the shell respectively; the adjusting assembly comprises an operation part rotationally arranged at one end of the shell, a transmission part rotationally arranged in the shell in the length direction of the shell and a telescopic part arranged in the shell, the power input end of the transmission part freely penetrates out of the shell and then is connected with the operation part, and the power output end of the transmission part is connected with the telescopic part; the telescopic end of the telescopic piece is connected with the plurality of planting assemblies; the operation part is rotated to drive the telescopic end of the telescopic part to move through the transmission part so as to change the distance between the planting assemblies.

The utility model relates to the technical field of sprinkling irrigation devices, and discloses a citrus planting lifting type sprinkling irrigation device which comprises a supporting rod, a fixing plate is fixedly installed at the upper end of the supporting rod, a first telescopic rod is fixedly installed at the upper end of the fixing plate, a water guide plate is fixedly arranged on the upper surface of the first telescopic rod, and an irrigation spray head is fixedly installed at the upper end of the water guide plate; a water inlet pipe penetrates through the side face of the water guide plate, a second telescopic rod is fixedly installed at the upper end of the water guide plate, a bearing plate is fixedly installed at the lower end of the supporting rod, and a threaded hole is formed in the bearing plate. According to the lifting type sprinkling irrigation device for citrus planting, the irrigation spray head is arranged to be of a lifting structure, the appropriate sprinkling irrigation height can be selected according to the growth height of citrus plants, the sprinkling irrigation effect on the citrus plants is guaranteed, a pressure sensing structure is additionally arranged at the lower end of the supporting rod, and the ground of the placement position can be continuously detected after the citrus plants are placed; and when the ground is scoured to a dangerous situation, alarm processing can be carried out in time, and the situation that the whole sprinkling irrigation device topples over is avoided.

The invention relates to the technical field of agricultural biological genes, and discloses a CsCRK10 gene, application of encoded protein and a method for improving citrus canker resistance, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsCRK10 gene in the citrus plants; the method specifically comprises the following steps: (1) cloning a citrus CsCRK10 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsCRK10 gene expression level. By cloning the coding sequence of the CsCRK10 gene of the citrus, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsCRK10 overexpression transgenic plant can be reduced to 66.17% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the method is suitable for industrial production. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The application discloses a DPO RT-PCR primer group for synchronously detecting five citrus viruses, a detection method, a kit and application of the DPO RT-PCR primer group, and sequences of the primer group are shown as SEQ ID NO. 1-SEQ ID NO. 10. The application designs specific DPO RT-PCR primers for citrus tristeza virus, citrus tatter leaf virus, citrus yellow vein clearing virus and citrus leaf mottle virus, and the detection primers can effectively remove primer dimer interference, and the five viruses can be detected from samples at the same time. The detection method is simple and efficient, and only needs to use a common PCR instrument and an electrophoresis instrument to detect, and has the characteristics of rapidness, economy, high accuracy and high sensitivity, and can be used for large-scale screening of field citrus plants and epidemic prevention detection before seedling transportation, and provides technical support for healthy seedling detection and field screening of diseases.

The present invention relates to the field of agricultural biological gene technology, and discloses a method for improving the resistance of citrus to citrus canker by regulating the expression level of CsSERK1 protein in citrus plants. Specifically, it includes the following steps: (1) cloning the CsSERK1 gene sequence of citrus; (2) constructing an overexpression vector; (3) transforming citrus with the overexpression vector to obtain transgenic plants with up-regulated expression level of CsSERK1 gene. By cloning the coding sequence of the citrus CsSERK1 gene, constructing an overexpression vector, and then transforming citrus, the incidence of citrus canker in the obtained CsSERK1 overexpression transgenic plants can be reduced to 51.74% of that of existing citrus at most, which can effectively and significantly reduce the incidence of citrus canker, reduce the lesion area, and does not affect the phenotype of transgenic plants. It has great application value for citrus canker resistance breeding and can be used as a candidate gene for citrus canker resistance breeding with multiple canker-resistant and -susceptible genes.

The application discloses a method for improving the resistance of citrus to Huangyanbing by using ClPsaN gene, comprising the following steps: (1) cloning a VIGS fragment of the citrus ClPsaN gene; (2) constructing two VIGS expression vectors of TRV and CLBV; and (3) transforming the VIGS expression vector into citrus to obtain VIGS plants in which the citrus ClPsaN gene is silenced. According to the method, the VIGS vector of the citrus ClPsaN gene is constructed, then the vector is transformed into citrus, and the obtained citrus plants can obviously show resistance to Huangyanbing. The titer of Huangyanbing, i.e., Huangyanbing yellow vein-illness virus, can be reduced by 22-34%, and the accumulation amount of virus protein can be reduced by 35-50%, so that the incidence of Huangyanbing is significantly reduced. In addition, the TRV silencing of the citrus ClPsaN gene does not affect the phenotype of the citrus plants, and the citrus ClPsaN gene can be used as a candidate gene to be used in the breeding of citrus plants resistant to Huangyanbing by using VIGS silencing, RNA interference and gene editing technologies in cooperation with multiple genes of the citrus plants resistant to or susceptible to Huangyanbing.

The utility model relates to the technical field of insect prevention devices, and discloses an insect prevention device for a citrus planting greenhouse, which comprises an outer shell and an insect prevention box, the upper end of the outer shell is fixedly provided with a fixed column, the lower end of the outer shell is movably provided with a material conveying port, one end of the material conveying port is movably connected with an insect killing box, and the other end of the material conveying port is movably connected with a water pump. A flame projector is fixedly installed on one side of the inner wall of the deinsectization box, a material conveying opening is movably sleeved with a hose, the other end of the hose is movably sleeved with a connecting pipe, the lower end of the connecting pipe is movably connected with a two-way air suction pump, and a sliding way is formed in a groove in the lower end of the outer shell. The sliding head and the sliding way are used in cooperation, the sliding head can be driven to move on the sliding way by means of free movement of the sliding wheel of the sliding head on the sliding way, the sliding head is fixedly connected with the insect prevention box through the connecting rod, the insect prevention box can move within a certain range below the outer shell, the insect killing range can be widened, and therefore the insect killing rate of the device is improved.

The invention provides a citrus fruit yield prediction and adjustment method and system, and relates to the technical field of yield prediction, and the method comprises the following steps: obtaining the yield demand of citrus, a citrus fruit remote sensing image and citrus planting parameters; processing the citrus fruit remote sensing image to obtain the predicted yield of the citrus and the growth period of the citrus; adjusting the citrus planting parameters according to the predicted yield and the yield demand; according to the invention, multi-source information fusion processing from citrus planting to picking is carried out and is combined with related planting requirements, and a yield adjustment strategy is provided.

The application provides a method for improving the resistance of citrus to canker disease based on gene interference, which comprises the following steps: introducing a long-chain fatty alcohol oxidase gene in a citrus plant into an interference vector as an interference sequence, and integrating the interference sequence into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. CsFAO3 The part of the coding sequence of the gene, i.e., the sequence as shown in SEQ ID NO: 3, is used as an interference sequence, introduced into an interference vector, and integrated into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. The method can directly inhibit most of the pathogenic bacteria causing the canker disease of the citrus, the gene to be interfered with can greatly improve the resistance of the citrus to the canker disease while reducing the adverse effects on the known quality and performance of the citrus to the greatest extent, a new way for the breeding of the citrus resistant to the canker disease is opened up, and the development of the breeding of the citrus resistant to the canker disease is promoted.

This invention discloses a butterfly adult directional attractant based on volatile chemical substances, along with its preparation and application methods. The invention uses a combination of readily available chemical reagents, such as linalool, as volatile signaling substances to efficiently induce various butterfly adults, including the Common Mormon and the Citrus Swallowtail, which use citrus plants (Rutaceae family) as hosts, to complete forward directional movements through flight or walking. This method can induce the synchronized directional movements of multiple butterflies of various species at a relatively low cost, and has significant practical value for human-butterfly interaction projects in ecological landscape construction and recreational experience settings.

The invention discloses a nerve calming and sleep promoting incense, which has treatment and health care potential, and citrus plants such as citrus, bergamot, grapefruit and the like are considered to have soothing and relaxing effects; plants such as pepper, geranium and lavender are considered to have the characteristics of calming and promoting sleep; pogostemon cablin, cedar, vetiver grass and other plants may help relieve anxiety and pressure.

The invention relates to the technical field of plant squeezing, and discloses plant squeezing equipment for essence refining, which comprises a support and a screen drum rotatably arranged on the support, and a feeding auger for extracting citrus plant essence, a driving part and a cleaning part arranged on the support are arranged in the screen drum. A plurality of through holes are formed in the circumferential outer wall of the screen drum, frustums are irregularly arranged on the circumferential inner wall of the screen drum, the feeding auger is divided into a shaft rod and spiral blades, the diameter of the shaft rod is gradually increased from front to back, a cavity is formed in the shaft rod, and a puncturing piece is arranged in the cavity. The plant squeezing equipment for essence refining can effectively solve the problems that in the prior art, due to the fact that the squeezing equipment completely stops working in the residue removing period, the production takt is obviously stopped, the smoothness of the whole squeezing process is directly damaged due to a discontinuous working mode, and the production efficiency is greatly improved. The problems of low equipment output efficiency and unstable essential oil extraction are further caused.

The invention provides miR1134 for regulating and controlling the wax content of citrus epidermis and the stress resistance of citrus as well as a short tandem target mimic molecule and application thereof, and relates to the technical field of biology. The invention provides miR1134 derived from Neuron navel orange, and the nucleotide sequence of the miR1134 is shown as SEQ ID NO: 1. The invention also provides a short tandem target mimic molecule for inhibiting the expression level of the miR1134, and the nucleotide sequence of the short tandem target mimic molecule is as shown in SEQ ID NO: 3. The short tandem target mimic molecule is introduced into a target plant through an STTM technology, and the citrus epidermis wax content is increased by inhibiting the expression level of miR1134, so that the drought tolerance and / or salt tolerance of citrus plants are / is enhanced. The invention lays a theoretical foundation and provides high-quality gene resources for cultivating new stress-tolerant citrus varieties.

The application discloses a primer and a method for identifying whether citrus fruits have low-temperature color promoting potential. CitHB7 After the specific primer designed for the promoter is used for PCR amplification reaction on the DNA of the citrus plant, whether the fruit has the low-temperature color promoting potential after harvesting is judged according to the target band after amplification. The citrus plant with the 750 bp target band has the low-temperature color promoting potential after harvesting; the citrus plant with the 100 bp target band does not have the low-temperature color promoting potential. The method provided by the application can quickly identify whether the citrus fruit has the low-temperature color promoting potential, and provides guarantee for accurate application of the low-temperature color promoting technology in postharvest treatment of the citrus fruit.

This invention discloses a method for enhancing the high-temperature resistance of citrus through exogenous spraying of naringin, belonging to the field of agricultural technology. The method involves preparing naringin into a formulation and spraying it exogenously onto the leaves of citrus plants. Experiments show that after treatment with naringin, phenotypic damage such as wilting and curling of citrus seedling leaves under high-temperature stress is significantly alleviated; the maximum photochemical efficiency of photosystem II (Fv / Fm) is significantly increased; electrolyte leakage rate and malondialdehyde content are significantly reduced; and the accumulation of hydrogen peroxide and superoxide anions is effectively inhibited. This invention utilizes naturally sourced naringin to significantly enhance the tolerance of citrus to high-temperature stress through multiple mechanisms, including maintaining photosynthetic system function, protecting cell membrane integrity, inhibiting membrane lipid peroxidation, and scavenging reactive oxygen species. Furthermore, the method is simple to operate, environmentally friendly, and low-cost, making it suitable for stress-resistant citrus cultivation.

The present disclosure relates generally to methods and systems for controlling citrus greening disease in citrus plants, including for example orange trees.

The application discloses a kind of citrus psylla intelligent detection method and device based on deep learning, method includes the following steps: S1, acquisition citrus psylla color image under natural orchard scene, pre-treatment is carried out to acquisition image, and citrus psylla dataset is made;S2, improvement YOLOv5-s network model, using citrus psylla dataset is iteratively trained to improved YOLOv5-s network model, and optimal model is obtained and used as psyllid detection model;S3, using 2 pairs of servo steering engine is formed rotatable holder and carries variable zoom camera and forms image collector;Psyllid detection model is deployed to embedded device, and using the equipment control image collector;S4, the image obtained by image collector is input into psyllid detection model and is identified, determines whether there is citrus psylla in the image photographed and its position.The method and device of the application can be deployed in natural orchard, and the citrus plants in a certain distance range are efficiently and intelligently monitored to determine whether there is citrus psylla.